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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Ilmu Lingkungan Jurnal Ilmu Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Animal Production : Indonesian Journal of Animal Production BIOEDUSCIENCE Jurnal SOLMA Tropical Animal Science Journal International Journal of Community Service Learning ARSHI Veterinary Letters Hasanuddin Journal of Animal Science (HAJAS) International Journal of Animal Science Wahana Peternakan Jurnal Syntax Fusion : Jurnal Nasional Indonesia Media Pustakawan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Kedokteran Hewan Journal of Animal Research and Applied Science Triwikrama: Jurnal Ilmu Sosial Jurnal Agripet Indenesian Journal of Tropical Biology Indenesian Journal of Tropical Biology Lentera Negeri Biodiversitas Journal of Biological Diversity

Jakaria Jakaria

Departemen Ilmu Produksi Dan Teknologi Peternakan, Fakultas Peternakan, IPB University, Bogor

Author-ID : 290981

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Articles

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The Genetic Variability of Prolactin and Signal Transducers and Activators of Transcription 5A (STAT5A) Genes in Bali Cattle Komang Alit Paramitasari; Cece Sumantri; . Jakaria
Media Peternakan Vol. 38 No. 1 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this study was to identify the genetic variability of PRL and STAT5A genes in Bali cattle from Bali, West Nusa Tenggara (NTB), and South Sulawesi (SS) using PCR-RFLP method. A total of 262 Bali cattle were identified using RsaI (PRL) and AvaI (STAT5A) restriction enzymes. PRL gene exon 3, PRL gene exon 4, and STAT5A gene exon 7 amplifications resulted fragments with the lengths of 156 bp, 294 bp, 215 bp, respectively. Genotyping of PRL gene both at exon 3 and 4 produced three genotypes in Bali population and two genotypes in NTB and SS population. For the PRL gene, frequencies of A allele (exon 3) and G allele (exon 4) were dominant to the B allele (exon 3) and A allele (exon 4) across all populations. The results showed that STAT5A|AvaI loci had monomorphic C allele. Heterozygosity values were found low at both exons 3 and 4 of PRL gene in all population. Sequence analysis results of PRL gene both for exons 3 and 4 showed that there was a mutation between adenine (A) and guanine (G) bases in the RsaI recognized site, whereas in STAT5A gene we can confirm the existence of AvaI restriction site (C|CCGAG).Key words: bali cattle, genetic polymorphism, PCR-RFLP, prolactin gene, STAT5A gene

Detection of Factor XI Deficiency (FXID) and Complex Vertebral Malformation (CVM) in Bali Cattle Sri Wahyuni Siswanti; Cece Sumantri; Jakaria jakaria
Media Peternakan Vol. 37 No. 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Factor XI Deficiency (FXID) is caused by imperfect insertion of poly adenine which is resulted in introduction of premature stop codon in FXI gene. Substitution of guanine into thymine in SLC35A3 gene caused Complex Vertebral Malformation (CVM). The research was aimed to detect the presence or absence of a genetic defect mainly CVM using SLC35A3 gene and FXID using FXI gene in Indonesian Bali cattle. The presence of this genetic defect may have a significant economic impact on the breeding program. The research of genetic defect was done mostly in dairy cattle, but there was no report for screening of genetic defect in Bali cattle. In this study, 303 fresh blood samples and 22 semen samples which were collected from Indonesian Bali cattle breeding center (BPTU HMT Denpasar, BPT HMT Serading West Nusa Tenggara and district Barru South Sulawesi) and artificial insemination centre (BBIB Singosari and BIBD Baturiti) were used for screening of FXID and CVM. The amplicons of FXI gene were obtained by using PCR and that for SLC35A3 gene were obtained by using PCR-RFLP method with PstI restriction enzyme. These PCR products were analyzed by using 2% agarose gels electrophoresis. All genotypes were confirmed by DNA sequencing to determine an allele mutant. The allele mutant was not found in all of the samples. The result of this study showed that CVM and FXID were not detected in Bali cattle from Indonesian Bali cattle breeding and artificial insemination centres.Key words: Bali cattle, genetic defect, SLC35S3 gene, factor XI gene

The Variability of Growth Hormone Gene Associated with Ultrasound Imaging of Longissimus dorsi Muscle and Perirenal Fat in Rabbits T I Amalianingsih; B Brahmantiyo; . Jakaria
Media Peternakan Vol. 37 No. 1 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Identification of genes in rabbits correlated to economic traits were intended to improve and develop their genetic quality. The objective of this research was to analyze the variability of growth hormone gene (GH) in three rabbit breeds, i.e. Rex, Satin, and Reza (Rex and Satin crosses) then was associated with ultrasound imaging of Longissimus dorsi muscle and perirenal fat thickness. Identification of the variability of growth hormone gene was analyzed using PCR RFLP technique from blood samples of 33 mature male rabbits in Indonesian Research Institute for Animal Production (IRIAP). Thickness of Longissimus dorsi muscle and perirenal fat were imaged and measured by using ultrasound unit at 2nd to 3rd lumbar vertebrae in the left body side. PCR product of GH gene fragment (231 base pair /bp) was digested with restriction enzyme Bsh1236I. PCR-RFLP patterns were allele T resulted in an undigested fragment of 231 bp; allele C resulted in fragment of 169 bp and 62 bp. The result showed that Bsh1236I GH gene had three genotypes, i.e. CC, TT, and CT. There were signifficant association of Longissimus dorsi muscle thickness between rabbit breed (P<0.05). There was no significant association between GH Bsh1236I gene polymorphism and imaging ultrasound of Longissimus dorsi muscle and perirenal fat thickness. The association of characteristic genotype of GH|Bsh1236Igene with measurement phenotype was not significant, however it had potency as marker assisted selection (MAS). Key words: growth hormone gene, Longissimus dorsi, perirenal fat, rabbit, ultrasound

Genetic and Phenotypic Parameters of Body Weight in Ettawa Grade Goats F Hasan; . Jakaria; A Gunawan
Media Peternakan Vol. 37 No. 1 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this study was to estimate genetic and phenotypic parameters of growth traits consisting of birth weight (BW), weaning weight (WW), 6 mo body weight (6WM), 12 mo body weight (12WM), and 18 mo body weight (18WM) of Ettawa Grade goats. The number of goat used to determine growth traits of BW, WW, 6WM, 12WM, and 18WM were 316; 316; 259; 259 and 165 heads, respectively. Data were analyzed using General Linear Model (GLM) to identify non-genetic effect. Estimation of genetic and phenotypic parameters including heritability, repeatability, genetic and phenotypic correlation were calculated using Restricted Maximum Likelihood and GLM. Genetic trends were calculated using the regression of mean breeding values on birth year. The results showed that parity and type of birth had significant (P<0.05) influence on all growth traits. Estimated heritability of birth, weaning, 6WM, 12WM, and 18WM were 0.54±0.12; 0.35±0.07; 0.37±0.09; 0.68±0.16 and 0.63±0.19, respectively. Estimated repeatability of WW, 6WM, 12WM and 18WM, WW, 6WM, 12WM and 18WM were 0.98±0.01; 0.97±0.01; 0.94±0.03; 0.71±0.12 and 0.91±0.04, respectively. The genetic trends for traits of BW and 18MW were decreased fluctuatively. However, the WW, 6MW, 12MW were increased fluctuatively. The high and positive genetic correlations between all growth traits and 12WM traits in this study indicated that selection for high 12WM will improve genetic merit in Ettawa Grade goats. Key words: body weight, EBV, Ettawa Grade goat, genetic and phenotypic parameters

Genetic Diversity of Bali Cattle Based on Microsatellite Marker in Indonesian Breeding Centre Wike andre Septian; . Jakaria; Cece Sumantri
Media Peternakan Vol. 38 No. 1 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Genetics characterization in livestock based on microsatellite has been widely implemented including for Bali cattle in three different breeding centres in Indonesia. This study aimed to determine diversity of Bali cattle microsatellites in three breeding centers namely BPTU Bali cattle in Bali, BPT-HMT Serading Sumbawa in West Nusa Tenggara, and Village Breeding Center in South Sulawesi. The number of animals used in this study was 95 head of cattle consisted of 32 heads from BPTU Bali Province, 32 heads from BPT-HMT Serading Sumbawa, and 31 heads from Village Barru Breeding Center Barru distric. Microsatellite loci used to determine the diversity was the locus SPS115, INRA037, MM12, and ETH185 based on flourescently labeled fragment method. Data analysis of microsatellite in Bali cattle at three different locations was performed by using POPGEN 1.2, Cervus, and POPTREE2 programs. The results showed that microsatellite diversity in Bali cattle detected 32 alleles from three different locations, and there were specific alleles at each location. Average values of observed heterozygosity (Ho) and expected heterozygosity (He) were 0.418 and 0.604 respectively, while the average value of polymorphism informative content (PIC) was 0.579. The Hardy-Weinberg equilibrium in general suggested that the loci used in the Bali cattle in three populations were in equilibrium, except INRA037 and ETH185. The genetic diversity between populations of Bali cattle was 0.033 (3%), while the inbreeding coefficient index in all populations was 0.296 (29.6 %). Bali cattle phylogeny tree with three populations showed that the populations of Bali cattle in BPTU Bali and VBC Barru had close genetic distance compared to the population of Bali cattle in BPT-HMT Serading Sumbawa. The results of this study provide information that the characteristics of Bali cattle breeding centers in three locations are different, so we need a directed breeding program in each population.Key words: Bali cattle, genetics diversity, microsatellite

Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle R. Putri; R. Priyanto; A. Gunawan; . Jakaria
Media Peternakan Vol. 38 No. 3 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Calpastatin (CAST) gene is well known as an inhibitor of muscle protein degradation and relates to muscle growth and meat tenderness. The objective of this study was to determine the association of CAST gene with growth traits and carcass characteristics in Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali Cattle to obtain growth traits, carcass characteristics, and blood samples. Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA sequencing. The association of CAST gene with growth traits and carcass characteristics were analyzed by using General Linear Model (GLM). The result showed that there were two genotypes (GG and AG) of CAST gene with allele frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG of CAST gene significantly affected (P<0.05) the back-fat thickness and longissimus dorsi without a significant effect on the growth traits. It could be concluded that CAST gene had a potency as a marker gene for carcass quality in Bali cattle. Key words: Bali cattle, CAST gene, PCR-RFLP, polymorphism

Identification of a Single Nucleotide Polymorphism at Hinf-1 Enzyme Restriction Site of Pit-1 Gene on Indonesian Bali Cattle Population Jakaria Jakaria; Ronny R Noor
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study aimed to determine the Pit-1|Hinf-1 gene polymorphism in Bali cattle (Bos javanicus) as Indonesian native cattle and besides Madura, Pesisir, Aceh, and Katingan cattle breeds as a comparison. DNA samples were extracted from 488 blood samples consisting of Bali (245 heads), Madura (68 heads), Aceh (25 heads), Pesisir (100 heads) and Katingan (50 heads) cattle. The diversity of the Pit-1|Hinf-1 gene wasere analyzed using PCR-RFLP. Whereas the nucleotide base mutations were identified by sequencing. Genotyping data were analyzed by calculating the allele frequency, observed heterozygosity (Ho) and expected heterozygosity (He) values as well as Hardry-Weinberg equilibrium test using POPGENE 1.31 program. Whereas, Tthe sequence data were analyzed by using MEGA6 program. The Pit-1|Hinf-1 gene fragment analysis showed that Bali, Madura, Pesisir, Aceh, and Katingan cattle had high BB genotype, resulting in B allele frequency of 0.982, 0.963, 0.925, 0.960, and 0.960, respectively. Ho and He values were 0.074-0.130 and 0.036-0.139, respectively. Hardy-Weinberg equilibrium test did not significant for all breed populations, except for Aceh cattle population (P<0.05). Mutation from guanine (G) to adenine (A) was found in Pit-1 gene fragment. Therefore, Pit-1|Hinf-1 gene fragment had low genetic diversity in Bali cattle and other breeds population.Key words: Bali cattle, Pit-1|Hinf-1 gene, polymorphism

Supplementation of Mangosteen Pericarp Meal and Vitamin E on Egg Quality and Blood Profile of Laying Hens R. K. Rusli; K. G. Wiryawan; T. Toharmat; . Jakaria; R. Mutia
Media Peternakan Vol. 38 No. 3 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.512 KB)

This research aimed to study the supplementation effects of mangosteen pericarp meal (MPM) and vitamin E (VE) in the diets on the egg quality and blood profile of laying hens. This research used 160 laying hens of Lohman strains 24 weeks of age. The observation was conducted for 11 weeks. A completely randomized design with four treatments and four replications (10 birds each) was used in this experiment. The treatments consisted of R0 (control diet), R1 (R0 + 1 g MPM/kg ration), R2 (R0 + 2 g MPM/kg ration) and R3 (R0 + 200 mg VE/kg ration). Variables measured were egg quality, yolk cholesterol, and blood profiles. The data were analyzed by using analysis of variance (ANOVA) and any significant difference between the treatment means were further tested by Duncan’s Multiple Range Test. The results showed that supplementation of 1 g MPM/kg ration in the diet significantly (P<0.05) decreased blood triglycerides compared with the control, laying hens fed with diet suplemented with of 2 g MPM/kg ration, and laying hens with diet suplemented with 200 mg VE/kg ration. Supplementation of MPM and VE did not affect (P>0.05) egg quality (except shell thickness), blood cholesterol, and HDL, respectively. In conclusion, supplementation of 1 g MPM/kg in the diet of laying hens could decrease blood triglycerides.Key words: blood profile, laying hens, mangosteen pericarp meal, egg quality, vitamin E

Identification of Rabbit Myostatin Gene Polymorphisms Tya I Amalianingsih; B. Brahmantiyo; . Jakaria
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The existence of selection on the rabbits with potential for meat has only been seen from phenotypic aspects including performance and productivity, while the molecular genetic studies are still very rare. One of the candidate genes for meat production traits in rabbit is myostatin. Totally 50 blood samples of male rabbits from Rex, Satin, Reza (crossing from Rex and Satin), Flemish Giant and FZ3 (crossing from Flemish Giant and Reza) breed were used at Indonesian Research Institute for Animal Production (IRIAP). Genetic polymorphism by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) method used FspBI restriction enzyme. PCR-RFLP data were analyzed by calculating allele and genotype frequencies. Sequencing was performed in rabbit with different genotypes which represents each of the samples. Genotype of AT had two cut points of the FspBI restriction enzyme at the base position of 508 bp and 444 bp. The cut point at the base position of 446 bp was site mutation base T became A. Genotype of TT had one cut point at the base position of 508 bp and no mutation site. Allele T had higher frequency than allele A and just Rex and Reza rabbit breeds had two alleles. The other rabbits (Satin, Flemish Giant and FZ3) only had one allele i.e., allele T. PCR - RFLP analysis of the MSTN|FspBI gene segments was polymorphic in Rex and Reza rabbit breeds. All of rabbit breeds in this study did not have AA genotype.Key words: myostatin gene, rabbit

Identifikasi Gen Babi Melalui Runutan Klon cDNA Ovari . Jakaria; K Suzuki
Media Peternakan Vol. 26 No. 1 (2003): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Identifikasi Gen babi melalui runutan klon cDNA ovari

Co-Authors . Aryogi . Aryogi, . . Hartati . hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. GUNAWAN A. Gunawan A. Gunawan A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Adelaide Jose Pereira Cristovao Adityo Raynaldo Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bambang Hermawan Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Budiman C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. A. Sari D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Darwati, Sri DEWI APRI ASTUTI E Andreas E Sari E. Andreas Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas EVA AMALIA PERTIWI F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti FADHIL MUHARRAM Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. G. R. Putra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro I. Suhendro I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Khasanah, H. Kholijah Kocu, N. Komang Alit Paramitasari M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur MASKUR MASKUR Mirza Dikari Kusrini Mohamad Yamin Mohammad Mukhlis Kamal Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHAMMAD MUHSININ Muhammad Nico Ghossani MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Mulyono, Rini Herlina Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nadya Nurafifah Andriya Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putri Indah Ningtias R R Noor R Sari R. Afnan R. F. Istiqlal R. H. Mulyono R. Iis Arifiantini R. K. Rusli R. Misrianti R. Mutia R. Mutia R. Oktaviani R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor R.R. Noor Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Riska Dwi Nanda Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. H. Wijaya S. Hartono S. M. Rachmawati S. N. Karimah S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik . Sarwi, Annisa Sony Hartono Wijaya Sri Rahayu Sri Sulandari SRI SULANDARI Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Surya Kusuma Wijaya Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TAPAUL ROZI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. G. M. Bonawati Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari

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