Claim Missing Document
Check
Articles

Polymorphism of ADIPOQ and EDG1 genes in Indonesian beef cattle Sutikno, S.; Priyanto, R.; Sumantri, C.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.323-332

Abstract

The ADIPOQ and EDG1 genes were responsible in intramuscular fat deposition and marbling scores. This study was aimed to identify polymorphism of indel g.81966364D>I in promoter region of ADIPOQ gene and SNP c.-312A>G in 5' UTR of EDG1 gene in Indonesian beef cattle. Blood samples were collected from 211 cattle, including Bali (44), Madura (20), Pesisir (18), Katingan (20), PO (22), Pasundan (20), SO (12), Brahman (20), Simmental (15) and Limousin (18). Polymorphism of ADIPOQ gene was analyzed using PCR and direct sequencingmethods, whereas EDG1 gene was analyzed using PCR-RFLP (MscI enzyme) and direct sequencingmethods. Results of genotyping indel g.81966364D>I was monomorphic (DD genotype). The SNP c.-312A>G was polymorphic (AA and AG genotype) in Madura, Pesisir, Pasundan, Brahman, and Limousine. The Frequencies of allele A and G were 0.95, 0.92, 0.98, 0.95, 0.94 and 0.05, 0.08, 0.02, 0.05, 0.06 respectively. The values of Ho and He were 0.05-0.17 and 0.05-0.15 respectively and in Hardy-Weinberg equilibrium (P>0.05). In Bali, Katingan, PO, SO and Simmental were monomorphic (GG genotype). In Bali cattle, two novel SNP candidates were found in position of c.-399C>T and c.-273C>G which were potential to be used as genetic markers of marbling score for Bali cattle. As result this study, it can be concluded that ADIPOQ gene was similar while EDG1 gene was different in Indonesian beef cattle. in addition, found two candidates potential SNP in Bali cattle.
ANALYSIS ON Alu-I GROWTH HORMONE (GHAlu-I) GENE IN BALI CATTLE Jakaria, J.; Noor, R.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.77-82

Abstract

The research was conducted to identify Alu-I locus of growth hormone (GH) gene in Bali cattle byusing 232 blood samples collected from Bali and Lombok islands. PCR-RFLP and sequencing methodswere used to detect the polymorphism and nucleotide sequence at Alu-I locus of GH gene. The resultshowed that Bali cattle from Bali island has one genotype (LL genotype), whereas Bali cattle originatingfrom Lombok island has two genotypes, namely LL and VV genotypes, respectively. The L and V allelefrequencies from Bali and Lombok islands were 1.00 and 0.00; 0.99 and 0.01, respectively. Thesequencing result of Bali cattle LL genotype showed an AGCT sequence of enzyme Alu-I restriction site.Based on polymorphic informative content (PIC) value, it can be concluded that Alu-I locus of Balicattle from Bali and Lombok islands were monomorphic and polymorphic, respectively.
Prediction of meat quality in Bali cattle using ultrasound imaging Jakaria, J.; Khasanah, H.; Priyanto, R.; Baihaqi, M.; Ulum, M. F.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 2 (2017): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.2.59-65

Abstract

The objective of this study were to predict carcass quality characteristics in Bali cattle using ultrasound imagery. The Number of samples were 81 heads of Bali cattle consist of bulls (62 heads) and cows (19 heads) with various age ranging from 1 to 6 years were collected their body weight and carcass qualities including backfat thickness (BF), longissmus dorsi thickness (LD), rump fat thickness (RF), rump thickness (RT), marbling score (MS) and the percentage of intramuscular fat (PIF). Those were estimated using ultrasound performed on 4.5-6,5 MHz frequency with depth of 8.8-13 cm. The BF, LD, MS and PIF measurement were applied on 12th-13th ribs, while the RT and RF measurement were conducted between ischium and illium. MS determination was calculated using Aus-Meat standard, while PIF was analysis based on Deaton and Rouse (2000). Body weight and carcass quality among traits were analyzed using descriptive and correlation procedures. The results showed that performance of body weight and carcass quality differs between Bali bulls and Bali cows, as well as among the age variations. Correlation analyses among traits (body weight and carcass quality) showed strong positive correlation (P<0.05) ranging from 0.291 to 0.938. In conclusion, ultrasound imaging method could be used to estimate carcass quality characteristics in Bali cattle.
Asosiasi Gen Myostatin Ekson 3 dengan Sifat Pertumbuhan pada Sapi Bali Wenny Ladhunka Nur Aliyya; Jakaria
International Journal of Animal Science Vol. 5 No. 01 (2022): International Journal of Animal Science
Publisher : Litbang PEMAS Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/asj.v5i01.86

Abstract

Penelitian ini dilaksanakan pada bulan Januari 2020. Tujuan dari penelitian ini adalah untuk mengidentifikasi keragaman SNP (Single Nucleotide Polymorphism) gen myostatin di daerah ekson 3 dan asosiasinya dengan sifat pertumbuhan pada sapi Bali. Materi penelitian menggunakan sampel darah diambil dari 52 ekor sapi bali jantan berasal dari BPTU-HPT Denpasar. Analisis DNA dilakukan secara bertahap yaitu mulai dari mengekstraksi DNA, mengamplifikasi gen MSTN dengan metode PCR dan melakukan sekuensing. Data hasil sekuensing selanjutnya dianalisis dengan menggunakan FinchTV, BioEdit dan MEGA7. Amplifikasi gen MSTN di daerah ekson 3 pada sapi bali berhasil dilakukan pada suhu 60 oC dengan panjang produk 451 pb. Dalam penelitian ini ditemukan 13 SNP dengan Frekuensi alel SNP yang ditemukan berada dalam keadaan equilibrium. Hasil asosiasi SNP Terdapat 3 SNP yaitu c.4900 T>G, c.4905 C>G dan c.4907 C>G yang berpengaruh nyata (P<0.05) terhadap bobot lahir pada sapi bali. Hasil penelitian yang mengungkap hubungan antara beberapa SNP gen MSTN yang berasosiasi dengan bobot lahir diharapkan dapat dijadikan sebagai kandidat penanda genetik bobot lahir sapi bali.
Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP . Jakaria; D Duryadi; R R Noor; B Tappa; H Martojo
Media Peternakan Vol. 30 No. 1 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.296 KB)

Abstract

A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined. The GH MspI gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH MspI and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP GH MspI marker has higher genetic variability compare to PCR-RFLP AluI marker. This finding showed that GH MspI T allele was favorable as a GH marker for Bos indicus breeds. Key words: Pesisir cattle, growth hormone gene, PCR-RFLP, polymorphism
Genetic and Non-Genetics Effect on Birth, Weaning, and Yearling Weight of Bali Cattle A Gunawan; . Jakaria
Media Peternakan Vol. 34 No. 2 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.664 KB) | DOI: 10.5398/medpet.2011.34.2.93

Abstract

The aim of this study was to evaluate the effect of genetic and non-genetic factors on the growth traits including birth weight, weaning, and yearling weight of Bali cattle. Data were analyzed using generalized linear model (SAS) to observe non-genetic effect. To evaluate the genetic effect, the estimation of heritability were done using mixed models analysis with the dam and sire as random effect and sex, parity, year of birth, and season as fixed effect in the model besides the residual. The results showed that sex of calf had no significant influence on birth and weaning weight but had significant influence on yearling weight (P+0.07, 0.33+0.09, and 0.43+0.10 respectively. Heritability value of growth trait weaning and yearling weight in Bali cattle was quite moderate to high, so it was expected that selection in achieving increased gain on growth trait was effective.
Estimates of Genetic and Phenotypic Trends of Growth Traits in Bali Cattle A Gunawan; R Sari; . Jakaria
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.58 KB) | DOI: 10.5398/medpet.2012.35.2.85

Abstract

The aim of this study was to estimate genetic and phenotypic trends for growth traits including birth (BW), weaning (WW) and yearling weight (YW) in Bali cattle. The number of cattle used to determine growth traits of BW, WW, and YW were 235, 215, and 178 heads, respectively. Estimation of breeding value, phenotypic and genetic correlation were calculated by Restricted Maximum Likelihood and General Linier Model (GLM) procedures, respectively. Genetic trends analysis was performed using the regression mean breeding values on birth year. Phenotypic and genetic correlation among BW and WW were 0.10 and 0.08 respectively and 0.90 and 0.70 for WW and YW respectively. The phenotypic trends for traits of birth and weaning weight were constant, whereas yearling weight was fluctuating from 2000 to 2008. Likewise, in the case of genetic trends, the birth and weaning weight were constant from 2000 to 2008 except for WW in 2005, whereas the genetic trends for yearling weight showed a fluctuation of wide range. According to the breeding value estimated for all traits, the best was the sire No. 0565, whose breeding value for BW, WW and YW were +0.07, +2.79, and +10.25 kg, respectively higher than the mean value of the population. The genetic trends showed that there have been a significant and positive genetic improvement in all growth traits and indicate that selection would be effective. Genetic correlation between WW and YW was high (0.70) which indicates that the selection on weaning weight might also increase yearling weight in Bali cattle.
Avian Sex Determination Based on Chromo Helicase DNA-binding (CHD) Genes Using Polymerase Chain Reaction (PCR) I Khaerunnisa; E Sari; M Ulfah; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.753 KB) | DOI: 10.5398/medpet.2013.36.2.85

Abstract

Several bird species are sexually monomorphic. In this case, molecular approach is an efficient method for their sex determination. The sexes of monomorphic birds can be determined by PCR amplification of the CHD genes. CHD genes are preserved within avian Z and W sex chromosomes. The objective of this research was to determine sex of 21 individuals quails, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos based on CHD genes using two pairs of primers: 2550F-2718R and P2-P8. Samples from Kampong chickens and ducks were used as controls. PCR products were screened by agarose gel electrophoresis with ethidium bromide. Results showed that 2550F and 2718R primers could be used to identify sex of Kampong chickens, quails, ducks, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. Individuals showed double (ZW) and single (ZZ) bands were identified as females and males, respectively in Kampong chickens, quails, hill myna, salmon-crested cockatoos and yellow-crested cockatoos. Males and females in ducks and pigeons showed single band in different length of base pairs. P2 and P8 primers could be used to identify the sex of pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. All of those samples showed bouble (ZW) bands for females and single (ZZ) band for males.
Isolation and Characterization of Plantaricin Produced by Lactobacillus plantarum Strains (IIA-1A5, IIA-1B1, IIA-2B2) I I Arief; . Jakaria; T Suryati; Z Wulandari; E Andreas
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.508 KB) | DOI: 10.5398/medpet.2013.36.2.91

Abstract

Bacteriocins produced by Indonesian lactic acid bacteria Lactobacillus plantarum IIA-1A5, IIA-1B1, IIA-2B2 were purified and characterized. Plantaricin W gene had been successfully amplified from all strains. This amplicon showed the expected 200 bp size of plantaricin W gene. This bacteriocins purified from L. plantarum IIA-1A5, IIA-1B1, and IIA-2B2 were named plantaricin IIA-1A5, IIA-1B1, and IIA-2B2. Purification by cation exchange chromatography increased the purity (fold) and activity of plantaricins. Purity of plantaricin IIA-1A5 was increased by 3.13 fold with specific activity 13.40 AU/mg. Plantaricin IIA-1B1 had 2.98 fold purity with specific activity 5.12 AU/mg, while purity of plantaricin IIA-2B2 was 1.37 fold with specific activity 7.70 AU/mg. All plantaricins could inhibit the growth of pathogenic bacteria, such as Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus. Plantaricins could be digested by trypsin. Stability of plantaricins at 80 oC for 30 min and at 121 oC for 15 min were affected by type of plantaricin and species of pathogenic bacteria. Generally, plantaricin IIA-1A5 was better as antimicrobial agent than plantaricin IIA-1B1 and plantaricin IIA-2B2.
Genetic Variation of mtDNA Cytochrome Oxidase Subunit I (COI) in Local Swamp Buffaloes in Indonesia F Saputra; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.336 KB) | DOI: 10.5398/medpet.2013.36.3.165

Abstract

The objective of this research was to identify genetic variation of mitochondria DNA especially in cytochrome oxidase subunit I (COI) among population of Indonesian buffaloes. Samples of swamp buffaloes were collected from Aceh (n= 3), North Sumatra (n= 3), Riau (n= 3), Banten (n= 3), Central Java (n= 3), West Nusa Tenggara (n= 3) and South Sulawesi (n= 3), and riverine buffalo from North Sumatra (n= 1) out of group for comparison. Sequence of COI was analyzed using MEGA 5.10 software with neighbor-joining method kimura 2-parameter model to reconstruct phylogeny tree. The result showed that three haplotypes for swamp buffalo and one haplotype for riverine buffalo in Indonesia resulted from 41 polymorphic sites. This finding showed that the COI gene could be considered as a marker to distinguish among swamp buffaloes in Indonesia.
Co-Authors . Aryogi . Aryogi, . . Hartati . hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. Gunawan A. GUNAWAN A. Gunawan A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Adelaide Jose Pereira Cristovao Adityo Raynaldo Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bambang Hermawan Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Budiman C. Sumantri C. Sumantri C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. A. Sari D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Darwati, Sri DEWI APRI ASTUTI E Andreas E Sari E. Andreas Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas EVA AMALIA PERTIWI F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti FADHIL MUHARRAM Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. G. R. Putra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro I. Suhendro I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Khasanah, H. Kholijah Kocu, N. Komang Alit Paramitasari M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur MASKUR MASKUR Mirza Dikari Kusrini Mohamad Yamin Mohammad Mukhlis Kamal Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHAMMAD MUHSININ Muhammad Nico Ghossani MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Mulyono, Rini Herlina Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nadya Nurafifah Andriya Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putri Indah Ningtias R R Noor R Sari R. Afnan R. F. Istiqlal R. H. Mulyono R. Iis Arifiantini R. K. Rusli R. Misrianti R. Mutia R. Mutia R. Oktaviani R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor R.R. Noor Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Riska Dwi Nanda Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. H. Wijaya S. Hartono S. M. Rachmawati S. N. Karimah S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik . Sarwi, Annisa Sony Hartono Wijaya Sri Rahayu SRI SULANDARI Sri Sulandari Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Surya Kusuma Wijaya Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TAPAUL ROZI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. G. M. Bonawati Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari