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Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle Himmatul Khasanah; A. Gunawan; R. Priyanto; M. F. Ulum; Jakaria Jakaria
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.226 KB) | DOI: 10.5398/medpet.2016.39.2.95

Abstract

Myostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P≤0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P≤0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality.
Analisis interaksi genotipe-lingkungan pada beberapa sifat kuantitatif ulat sutra (Bombyx mori L) Jakaria Jakaria; S S Mansjoer; A Saefuddin; M Kaomini
Media Peternakan Vol. 24 No. 2 (2001): Media Peternakan (Supplement Edition: Animal Production)
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Analisis interaksi genotipe-lingkungan pada beberapa sifat kuantitatif ulat sutra (Bombyx mori L)
The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross I. Khaerunnisa; Jakaria Jakaria; I. I. Arief; C. Budiman; C. Sumantri
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1406.765 KB) | DOI: 10.5398/medpet.2017.40.2.78

Abstract

The chicken growth hormone (GH) and its receptor (growth hormone receptor, GHR) play important roles in chicken performances due to their crucial functions in growth. The variations of GH and GHR genes were then thought to be associated with the variations of the performances. This experiment was designed to identify the g.2248G>A GH and the g.565G>A GHR loci polymorphisms and to evaluate their associations with carcass components in Kampung and broiler chicken cross. A total of 215 chickens including 4 chicken populations (Kampung, Cobb broiler, F1, and F2 Kampung x broiler chicken cross) were screened to identify polymorphism using PCR-RFLP technique with EcoRV and Eco72I restriction enzyme for GH and GHR loci, respectively. The carcass components were recorded at 26 weeks of age on F2 Kampung and broiler chicken cross (42 chickens) for association study. Both the g.2248G>A GH and the g.565G>A GHR loci were polymorphic with two alleles (G and A) and three genotypes (GG, AG, and AA). The GG genotype and the G allele of GH locus were predominant in all chicken populations. While in GHR locus, the AA genotype and the A allele were found to be higher in all chicken populations. The association study showed that the g.565G>A GHR locus polymorphism had significant effect on carcass components, including live weight, carcass weight, breast weight, thighs weight, breast muscle weight, and thighs muscle weight. There was no significant association was found between the g.2248G>A GH genotype and carcass components. It could be concluded that the g.2248G>A GH and the g.565G>A GHR loci were polymorphic in Kampung and broiler chicken cross and the g.565G>A GHR locus was accosiated with carcass components. This g.565G>A GHR SNP might be an important candidate marker for chicken growth and muscle mass improvement.
KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL Cece Sumantri; . Jakaria; Mohamad Yamin; Henny Nuraini; Bramada Winiar Putra; Eryk Andreas
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12).
Polimorfisme g.-371T>A Promotor Gen Miostatin pada Sapi Pedaging Indonesia Sutikno Sutikno; Rudy Priyanto; Cece Sumantri; Jakaria Jakaria
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.116 KB) | DOI: 10.18343/jipi.25.2.239

Abstract

Myostatin (MSTN) gene acts as a negative regulator of muscle growth. The aim of the present study was to identify polymorphism of g.-371T>A in promoter region of MSTN gene in Indonesian beef cattle. Blood samples were collected from 191 cattle, including Bali (BL) (42), Madura (MD) (20), Pesisir (PI) (17), Katingan (KT) (16), Ongole grade (PO) (22), Pasundan (PD) (14), Sumba ongole (SO) (10), Brahman (BH) (17), Simmental (SM) (15), and Limousin (LM) (18). Polymorphism of MSTN gene was analyzed using PCR-RFLP (DraI) and direct sequencing methods. Results of genotyping g-371T>A were polymorphic (TT, TA, and AA genotypes) in Simmental, SO, and Katingan. The frequencies of alleles T and A were 0.83; 0.90; 0.97 and 0.17; 0.10; 0.03 respectively. The values of Ho and He were 0.06–0.20 and 0.06–0.28, respectively. The results of this study are in Hardy-Weinberg equilibrium (P> 0.05). Whereas in PD, MD, PI, PO, BH, LM, and BL were monomorphic (TT genotype). The result of sequencing the promoter region of MSTN gene found that mutations transversion was occurred in T to A at g.-371. It was concluded that g.-371T>A of MSTN gene was polymorphic which was potential to be used as genetic markers of muscle growth in SM, SO, and KT cattle. Keywords: SNP g.-371T>A of MSTN gene, Sumba ongole cattle, Katingan cattle, Simmental cattle
Analisis Keterkaitan GEN BMP15, BMPR1B, dan KISS1 dengan Sifat Fekunditas pada Kambing Peranakan Etawah Betina Rini Herlina Mulyono; Cece Sumantri; Ronny Rachman Noor; . Jakaria; Dewi Apri Astuti
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.746 KB) | DOI: 10.18343/jipi.24.2.83

Abstract

Etawah grade goat is a result of grading up line between Kacang and Etawah goats, which well adapted to Indonesia’s humid tropical climate. This goat is a dual-purpose goat breed for milk and meat production. The objectives of this study were to evaluate the polymorphism of fecundity genes through the investigation of four candidate genes for prolificacy, i.e., BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) and their associations with the litter size of Etawah-Grade does. The diversity of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (introns 1) genes were analyzed by the PCR-RFLP technique. A total of 106 of DNA samples were collected from Etawah-Grade does at BPTU-HPT Pelaihari in Pelaihari, South Kalimantan (51 heads) and Cordero Farm in Bogor, West Java (55 heads). Genetic polymorphisms between the two Etawah-Grade groups were calculated as well as their allele and genotype frequencies and the Polymorphic Informative Content (PIC). The Kruskal-Wallis one-factor ranked analysis of variance was used to determine whether there was any effect of genotype of the fecundity genes on litter size. The polymorphic information content (PIC) estimated at BPTU-HPT Pelaihari was 0.313, whereas at Cordero farm was 0.174, and overall PIC was 0.244. There was no association between genotypes of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) genes and litter size. Results indicated that BMP15 (exon 1) and KISS1 (exon 1) genes were monomorphic. However, the other two loci showed polymorphisms. The BMPR1B (exon 1) locus had two genotypes with the frequency for CC (96.23%) and for GG (3.77%), and KISS1 (intron 1) locus had three genotypes with the frequency for TT (80.19%), AT (17.92%), and AA (1.89%).
Association of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattle Dairoh Dairoh; Jakaria Jakaria; Mokhamad Fakhrul Ulum; Andi Baso Lompengeng Ishak; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 2 (2021): June 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i2.2701

Abstract

Bali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that  SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.
Heritability estimation and non-genetic factors affecting production traits of Indonesian Ongole cross . Hartati; . Muladno; . Jakaria; R. Priyanto; A. Gunawan; . Aryogi; Chalid Talib
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.514 KB) | DOI: 10.14334/jitv.v20i3.1183

Abstract

Productivity data from 560 head of PO cattle have been collected for 9 years from 2004 until 2013 for evaluating heritability estimation and environmental factors affecting production traits of Indonesian Ongole cross from birth to yearling old. Heritability estimation were analyzed using varians analysis with data of paternal halfsib correlation. Varians component for to estimate of heritability value were analyzed using completely randomized design one - way classification. While a general linear model was used to analyze the environmental factors (sex, year, parity, season and type of births). The results showed that sex of calves and year of births had significant differences (P<0.05) for all production traits such as weight at birth, weaning and yearling, while the type of birth only presented a significant difference on birth weight. Parity, season and generation exhibited nonsignificantly effect on those production traits. Estimation of heritability on birth weight, weaning weight and yearling weight were 0.28+0.12; 0.47+0.15 and 0.63+0.17 respectively. The highest heritability values obtained in yearling weight, this means that yearling weight will be effective as selection criteria to improve the genetic of PO cattle.
Fluctuating Asymmetry Increases with Heat Stress Disruptions on Bali Cattle (Bos javanicus) at Different Altitude Ikhsan Suhendro; Jakaria Jakaria; Rudy Priyanto; Wasmen Manalu; Ronny Rachman Noor
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2758

Abstract

Bali cattle (Bos javanicus) are common species distributed throughout Indonesia to survive in tropical hot, and humid climates. Fluctuating asymmetry (FA) is a random deviation from perfect bilateral symmetry. FA is often used to measure developmental stability in individuals and can be used as a marker for the impact of environmental and genetic stress. This study aims to investigate the pattern of developmental instability in Bali cattle as caused by environmental stress at various altitudes using the FA index. FA indexes in this study were used to compare asymmetrical physical traits of Bali cattle in Sembalun high altitude and Serading low altitude. Sixty-five Bali cattle were used in this study reared at different altitudes, namely Serading, Sumbawa Island (50 m above sea level), and Sembalun, Lombok Island (1,186 m above sea level). The physical traits of Bali cattle measured were horn circumference (horn), a distance of hip to spine bone (pelvic), metatarsal circumference (metatarsal), and metacarpal circumference (metacarpal). The FA1 and FA5 indexes showed significant metacarpal differences between Bali cattle reared at Sembalun and Serading population (P<0.05). FA11 multiple trait index as a composite of all traits revealed a significant difference as well. (P<001). It can be concluded that various heat stress by altitude impacts the asymmetry of Bali cattle.
Genetic and Non-Genetic Effects on Semen Characteristics of Bali Cattle (Bos javanicus) Gunawan Sitanggang; Raden Iis Arifiantini; J Jakaria
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2526

Abstract

The objective of this study was to evaluate effect of genetic and non-genetic factors on semen characteristics including ejaculate volume, sperm concentration, total sperm number and sperm moetility of Bali cattle. Semen data were collected from the National Centre of Artificial Insemination at Singosari, Malang, East Java, Indonesia. A total of 3,847 ejaculates of 17 Bali bulls from 2014 to 2016 were collected and evaluated. Data were analyzed by restricted maximum likelihood (REML) method using mixed models which the bull was a random effect, while age of bull, season of collection, frequency of ejaculation and collection intervals were the fixed effects. Results showed that age significantly affected all semen characteristics (P<0.01). Season affected only on sperm motility (P<0.01). Effect of frequency of ejaculation and collection intervals was significant on all studied variables (P<0.01), except sperm motility. Repeatability of ejaculate volume, sperm concentration, total sperm number and sperm motility was 0.43; 0.35; 0.32 and 0.31, respectively. It is concluded that age, frequency of ejaculation and collection intervals were the most factors affected semen characteristics of Bali cattle. Repeatability estimations of semen characteristics were moderate to high.
Co-Authors . Aryogi . Aryogi, . . hartati . Hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. Gunawan A. Gunawan A. GUNAWAN A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Adelaide Jose Pereira Cristovao Adityo Raynaldo Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bambang Hermawan Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Budiman C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. SUMANTRI C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. A. Sari D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Darwati, Sri DEWI APRI ASTUTI E Andreas E Sari E. Andreas EDWAR EDWAR Edwar Edwar Eka Haryati Yuliany Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas EVA AMALIA PERTIWI F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti FADHIL MUHARRAM Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. G. R. Putra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro I. Suhendro I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Khasanah, H. Kholijah Kocu, N. Komang Alit Paramitasari M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur MASKUR MASKUR Mirza Dikari Kusrini Mohamad Yamin Mohammad Mukhlis Kamal Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHAMMAD MUHSININ Muhammad Nico Ghossani MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Mulyono, Rini Herlina Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nadya Nurafifah Andriya Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putri Indah Ningtias R R Noor R Sari R. Afnan R. F. Istiqlal R. H. Mulyono R. Iis Arifiantini R. K. Rusli R. Misrianti R. Mutia R. Mutia R. Oktaviani R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor R.R. Noor Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Riska Dwi Nanda Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. H. Wijaya S. Hartono S. M. Rachmawati S. N. Karimah S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik . Sarwi, Annisa Sony Hartono Wijaya Sri Rahayu SRI SULANDARI Sri Sulandari Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Surya Kusuma Wijaya Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TAPAUL ROZI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. G. M. Bonawati Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari