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Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method Sarwi, Annisa; Noor, Ronny Rachman; Jakaria, Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.1

Abstract

This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP method. The investigation involved 40 blood samples from male Bali cattle aged 1.5 to 3 years, sourced from the Banjarmasin Slaughterhouse (RPH) in South Kalimantan. The SNP g.10428C>T diversity in the SCD gene was analyzed using the PCR-RFLP technique with the HinfI restriction enzyme. Genotype frequencies, allele frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium were calculated using Popgen32. The relationship between fatty acid composition and the genotype of the SCD gene, as well as the fatty acids in Bali beef, was analyzed using a t-test. SCD|HinfI was found to have homozygous CC (0.03) and TT (0.85) and heterozygous CT (0.13) genotypes with a C allele frequency of 0.09 and T of 0.91. SNP g.10428C>T in the SCD gene was not significantly associated with fatty acid composition.
Identifikasi Keragaman Gen Pituitary Transcription Factor 1 (Pou1f1) pada Kambing Peranakan Etawah (Pe) di BPTU KDI-HPT Pelaihari Suparman, A. B. P.; Mulyono, R. H.; Jakaria, Jakaria
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 3 No. 3 (2015): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Pituitary Tanscription Factor 1 (POU1F1) has been identified as the pituitary spesific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) in the anterior pituitary. The objective of this research is identification the polymorphism of POU1F1 gene of etawah grade goat in BPTU KDI-HPT Pelaihari South Kalimantan use Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). PCR products of POU1F1 digested with Hinf1 enzyme which restric G|AnTC.  Samples are used as the material of DNA from blood samples of 115 PE goats.  POU1F1 gene amplification was performed with annealing temperature of 62 oC for 20 seconds resulting in a fragment with the length of 601 bp which is located at intron 5 and exon 6. The result of PCR-RFLP analysis indicated that the POU1F1 gene was monomorphic.  B allele had 3 fragments at 275, 243, and 83 bp respectively.
Genetic snd Non-Genetic Effect on Gestation Length and Calf Survival at Weaning in Bali Cattle Gunawan, A.; Jakaria, Jakaria; Parwoto, Y.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 1 No. 3 (2013): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

The aim of this study was to evaluate effect of genetic and non genetic factor on the gestation length (GL) and calf survival to weaning (CS) of Bali cattle. Data were analyzed using Generalized Linier Model (SAS) to observe non-genetic effect. To evaluate the genetic effect, the estimations of heritability were done using Mixed Models analysis with the dam and sire as random effect and parity, year of birth and type of mating as fixed effect in the model besides the residual. The results showed non genetic factors except type of mating influenced the variability of gestation length (GL) and calf survival to weaning (CS) in Bali cattle. The result showed that dam year of birth and parity were found significant source of variation in the gestation length, whereas calf survival to weaning were affected of age of dam and year of birth.  Estimated heritability of  GL and CS were 0.68 ± 0.07  and 0.28 ± 0.09, respectively. With regards to genetic effect, estimated heritability of GL was considered higher (0.68), which means that GL information would be more effective as a selection criteria. Selection for shorter GL might be reduce calving problems with less impact on calf survival to weaning.
Identifikasi Ukuran Tubuh dan Bentuk Tubuh Sapi Bali di Beberapa Pusat Pembibitan Melalui Pendekatan Analisis Komponen Utama Hikmawaty, Hikmawaty; Gunawan, A.; Noor, R. R.; Jakaria, Jakaria
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 2 No. 1 (2014): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Bali cattle is indigenous livestock genetic resources which have distinctive features and ability to grow well in a variety of environments in Indonesia. The aim of this study was to determine the phenotypic profile of Bali cattle in some breeding centers which represented by BPTU Pulukan Bali, VBC Kab. Barru South Sulawesi and BPTHMT Serading NTB. Data were analyzed by using Principal Component Analysis (SAS) to determine the size identifier which was influenced by the environmental factors and genetic factors form  the basic information on the characteristics of Bali cattle. The parameters such as dimensions and body weight of Bali cattle due to the possibility of differences in genetic potential, location of origin, implementation of the mating system and environmental management including maintenance were observed. The result showed Bali cattle body size on BPTHMT Serading NTB  were smaller (P <0.5) compared to the body size of Bali cattle in BPTU Pulukan Bali and VBC Kab. Barru South Sulawesi while Bali cattle in BPTU Pulukan Bali and VBC Kab. Barru South Sulawesi were relatively similar. Chest girth measured on the body has the highest correlation with body weight in Bali cattle both in males and females in different breeding centers.The size score and the shape between Bali cattle breeding centers in the male and female Bali cattle showed its own characteristics due to their respective locations. Identifier the size of Bali cattle at NTB Serading BPTMHT showed relatively smaller than Bali Cattle in BPTU Pulukan Bali and VBC Kab. Barru SouthSulawesi. Identifier the same shape among all three populations showed the breeding centers have the samecharacteristic genetically.
Produktivitas Entok Betina dengan Pemberian Pakan Terbatas Selama Periode Pertumbuhan Ayuningtyas, G.; Jakaria, Jakaria; Rukmiasih, Rukmiasih; Budiman, C.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 4 No. 2 (2016): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

The objective of this research was to study the effect of restricted feeding on muscovy duck performance’s in grower period, the development of reproduction organ, puberty characteristic, egg production, and blood lavel of cholesterol and trigliseride. Total 32 of female muscovy duck , 8-22 weeks age ware evaluated in this research. A completely randomized design with 3 level treatments were used to arrange the treatments. The treatments of restricted feeding were ad libitum consumption ( P1); 70% ad libitum (P2) and 40 % ad libitum (P3). The diet contain 22% crude protein (0-3 weeks age), 18% crude protein (4-7 weeks age), and 14% crude protein (8-22 weeks age). The parameters observed were: performance’s in grower period (feed consumption, body weight, total daily gain), puberty characteristic (puberty age, body weight on puberty, first egg weight), egg production on first cycle, the development of reproduction organ, and blood lavel of cholesterol and trigliseride. The results showed that body weight of muscovy duck P2 and P3 lower then P1. The restricted feeding could delayed development of folicle ovary and  age puberty. The blood level of cholesterol’s muscovy duck P2 and P3 lower then moscovy duck P1.
Produktivitas Sapi Bali Betina dan Hasil Persilangannya dengan Limousin dan Simmental yang di Pelihara Berbasis Pakan Hijauan di Kabupaten Keerom Papua Kocu, N.; Priyanto, R.; Salundik, Salundik; Jakaria, Jakaria
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 7 No. 1 (2019): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Development of cattle in the Papua region still faces many obstacles, particularly the limited number of cattle population and the quality of the animal dominated by bali cattle. This study aimed to evaluate the productivity of bali cattle and their crosses with limousin and simental through artificial insemination. This study was carried out on December 2016 to February 2017  in the Arso and Skanto Districts, Keerom Regency, Papua. Ninty four heads of used in this study, comprising 57 bali catsle, 18 bali x limousin and 19  bali x simmental crosses through artificial insemination programe. Parameters vanahle included reproduction characteristics, body weight and linear body measurement.  The observation were conducted of calf (<12 month), young (13-24 month) and adult animal(>25 month).  The results indicated that the female bali cattle and their crosses with limousin and simmental raised on forage based feed showed different reproduction and production perfomances.  The cross bred cow (bali x limousin and bali x simmental) tended to have longer age at first mating, higher service per conception and lower calving rate.  Based on forage feed, the cross bred cow had significantly (P<0.05) higher body weight and body frame size, particularly body weght, hip height, body lengt and heart girh at adult age.  In general, the crosses between bali and limousin or simmental for cow-calf production could be developed on a forage based feed at Keerom District, Papua.
Karakteristik Morfometrik Sapi Aceh, Sapi PO dan Sapi Bali Berdasarkan Analisis Komponen Utama (AKU) Mahmudi, Mahmudi; Priyanto, R.; Jakaria, Jakaria
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 7 No. 1 (2019): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Aceh cattle  is one of four local cattle breeds (Aceh, Bali, Madura, and pesisir), and Ongole crossbreed is also regarded as the local cattle breed in Indonesia.  They are most likely to be well adapted to the environment, including disease, climate, feed and water availability. This study aims to determine the body size characteristics of Aceh, Bali, and Ongole crossbreed cattles. As many as 20 heads of Aceh, Bali, and Ongole crossbreed cattle respectively were used for measurement of 18 body parameters. Data obtained was then subkecyed to analysis of variance (ANOVA) and Principle Component Analysis (PCA). The results showed that morphometric measurements of Aceh, Ongole crossbreed, and Bali cattle were significantly different (P<0.05). PCA results showed that body measurement was detemined by chest circumference at Bali cattle, neck height at Ongole crossbreed, body length, and chest circumference at Aceh cattle. Meanwhile the shape components were chest widht at Ongole crossbreed and Bali cattle and thoracic part at Aceh cattle. This result should conribute to  phenotype profilinge of Bali cattle, Ongole crossbreed, and Aceh cattle, whic is important for cattle breeding and conservation in Indonesia.
Profil Hormon Progesteron dan Gen Fekunditas terhadap Sifat Kembar Kambing PE Betina Calon Induk Mulyono, R. H.; Sumantri, C.; Noor, R. R.; Jakaria, Jakaria; Astuti, D. A.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 6 No. 1 (2018): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Etawah-grade goat is a graded up line between kacang and etawah goats, which well adapted to Indonesia’s humid tropical climate. The good reproductive trait of Etawah-grade does are inherited from kacang goat (prolific goat). This study used six heads of Etawah-grade does at 1–2 years old (I1 dental condition). IPB University. The body variables were withers height, hip height, body length, chest width, chest depth, thurl width, rump length, chest girth, and cannon circumference, whereas for head variables were acrocranion–prosthion, basion–prosthion, lower jaw length, head height, tuber facial left-right, nasion–rhinion, entorbitale left-right, euryon left-right, supraorbitale left-right. Body score and head score were obtained from Principal Component Analysis (PCA). PCR-RFLP technique was used to determine genotypes of fecundity genes BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1) and KISS1 (intron 1) with restriction enzymes AluI (AG|CT), SduI (GDGCH|C), BsrI (ACTGG|) and MwoI (GCCTAAG|TAGC), respectively. Progesterone leves were measured by EIA (enzyme immuno assay). The results showed that the association between crude fat consumption and progesterone profile was found in this study, but it was not related to twin births with certain genotypes of gene fecundity BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1) and KISS1 (intron 1). The progesterone profile during the pregnancy period did not correlate to their body size and head size.
The TGF-β3 Gene Polymorphisms and Carcass Components in Kampung x Meat Type Chicken Cross I. Khaerunnisa; Jakaria; C. Sumantri
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 8 No. 1 (2020): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.8.1.42-47

Abstract

The TGF-β3 gene is a subtype of TGF-β superfamily that plays a crucial role in skeletal muscle growth. The objectives of this study were to identify the TGF-β3|BslI locus polymorphisms and to evaluate their effects on carcass components in Kampung and broiler chicken cross. A total of 129 chickens used in this study, consisting of: Kampung chickens (44), parent stock broilers Cobb strains (10), F1 Kampung x broilers (35), and F2 Kampung x broilers (40). The PCR-RFLP and direct sequencing techniques were used to obtain mutations in the intron 4 of TGF-β3 gene using the BslI restriction enzyme. The TGF-β3|BslI locus was polymorphic in all populations and three genotypes were found: CC, AA, and CA. No association was found between the three genotypes with the carcass components in F2 Kampung x broiler cross.
Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle Kholijah; Mokhmad Fakhrul Ulum; Sri Darwati; Ronny Rachman Noor; Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.05

Abstract

This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.
Co-Authors . Aryogi . Aryogi, . . Hartati . hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. GUNAWAN A. Gunawan A. Gunawan A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Adelaide Jose Pereira Cristovao Adityo Raynaldo Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bambang Hermawan Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Budiman C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. A. Sari D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Darwati, Sri DEWI APRI ASTUTI E Andreas E Sari E. Andreas Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas EVA AMALIA PERTIWI F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti FADHIL MUHARRAM Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. G. R. Putra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro I. Suhendro I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Khasanah, H. Kholijah Kocu, N. Komang Alit Paramitasari M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur MASKUR MASKUR Mirza Dikari Kusrini Mohamad Yamin Mohammad Mukhlis Kamal Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHAMMAD MUHSININ Muhammad Nico Ghossani MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Mulyono, Rini Herlina Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nadya Nurafifah Andriya Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putri Indah Ningtias R R Noor R Sari R. Afnan R. F. Istiqlal R. H. Mulyono R. Iis Arifiantini R. K. Rusli R. Misrianti R. Mutia R. Mutia R. Oktaviani R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor R.R. Noor Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Riska Dwi Nanda Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. H. Wijaya S. Hartono S. M. Rachmawati S. N. Karimah S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik . Sarwi, Annisa Sony Hartono Wijaya Sri Rahayu Sri Sulandari SRI SULANDARI Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Surya Kusuma Wijaya Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TAPAUL ROZI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. G. M. Bonawati Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari