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Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle Adefia Fahira; Ronny Rachman Noor; Jakaria Jakaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 3 (2022): September 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i3.3045

Abstract

PLAG1 gene is one of those that regulate growth and body size.  This study aimedto look at the PLAG1 gene polymorphism and its relationship to birth weight in Bali cattle using PCR-RFLP. The total sample used was 104 samples consisting of 66 Bali cattle from BPTU-HPT Denpasar and 38 Bali cattle from BPT-HMT Serading, each of which had birth weight data. PLAG1 gene polymorphism was analyzed using PCR-RFLP and the Sac1 restriction enzyme. The genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were all examined using Popgen32. General Linear Model was used to analyze the association of SNP 795AG PLAG1 gene with birth weight in Bali cattle. Amplification of the PLAG1 gene resulted in 776 bp fragments and two alleles. The PLAG1 gene had three genotypes: AA (562 bp and 182 bp), AG (562 bp, 182 bp, and 104 bp), and GG (562 bp, 182 bp, and 104 bp). Based on the results, the PLAG1 gene in Bali cattle was polymorphic. The alleles frequency of Bali cattle wasin Hardy-Weinberg equilibrium. The SNP c.795AG PLAG1 gene genotype were associated with birth weight in Bali cattle. The A allele is a determinant of high birth weight in Bali cattle where the AG genotype has the highest birth weight.
Determination of Complete Sequence Mutation of Myostatin Gene in Fast- and Slow-Growing Chicken Isyana Khaerunnisa; Ahmad Furqon; Saiful Anwar; Jakaria Jakaria; Cahyo Budiman; Irma Isnafia Arief; Cece Sumantri; Yong Soo Kim
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.148-158

Abstract

Myostatin plays a role in inhibiting skeletal muscle growth in vertebrates. This study aimed to investigate the full sequence of the myostatin gene in fast-growing and slow-growing chickens. Fast- and slow-growing chicken models were produced from F2 Kampung x broiler. The full sequence of the myostatin gene was identified using 24 pairs of primers covering about 8,000 bp. mRNA expression analysis of muscle tissue was performed to examine whether the expression levels of myostatin are affected by chicken lines, sex, or muscle type. The results showed 170 mutations in fast- and slow-growing chickens. One hundred and sixty-one of them are novel mutations. A total of five and twenty-two alleles were specific alleles found only in the fast-growing and slow-growing groups of chickens, respectively. There were no differences in amino acids and gene expression levels of myostatin between the fast- and slow-growing chickens. In summary, the results of this study showed that specific alleles for the fast-growing or slow-growing chicken groups were found, suggesting that these specific alleles potentially be used as genetic markers for muscle growth in chickens.
The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance I. Suhendro; J. Jakaria; R. Priyanto; W. Manalu; R. R. Noor
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.429

Abstract

Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T>G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T>G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp & 498 bp; GG with 144, 236, & 262 bp; and TG with 144, 236, 262, & 498 bp. Bali cattle with the GG genotype had lower (p<0.001) Rr and HTC compared to the other genotypes. It could be concluded that physiological performances were lower at high altitudes, and the SNP -69T>G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T>G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.
DIVERSITAS GENETIK DAN HAPLOGROUP KAMBING GEMBRONG BERSTATUS KRITIS DI KABUPATEN KARANGASEM, BALI (Genetic Diversity and Haplogroup of Endangered Gembrong Goat In Karangasem, Bali) M. Syamsul Arifin Zein; Sri Sulandari; Jakaria Jakaria; I Made Londra; Suprio Guntoro; Ida Bagus Gaga Partama
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.941 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5134

Abstract

This study was conducted to evaluate the genetic diversity and phylogeny of Gembrong goat. For this purpose, 21 goats from endangered breed in Karangasem Bali were used. Molecular analysis of genetic diversity and phylogeography used hypervariable segment 1 of mitochondrial DNA control region. The result showed that genetic variability of Gembrong goat was homogeneous with only one different sites, namely the substitution pyrimidines of C ↔ T (transitional). Phylogeny analysis results showed maternal origin of Gembrong goat is lineage (subhaplogroup) B1 with frequency of 100%. Haplogroup B were known has been domesticated from wild goat in western Asia, then headed to south Asia and infiltrated to southeast Asia, including Gembrong goat in Bali, Indonesia. As a conclusion, genetic diversity of Gembrong goat from remaining population in Karangasem very low and originate from lineages/haplogroup B1 with a frequency of 100%.
KERAGAMAN FENOTIPE SIFAT KUANTITATIF AYAM BURGO, AYAM HUTAN MERAH, DAN AYAM KAMPUNG DI PROVINSI BENGKULU: DIVERSITY OF PHENOTYPES OF QUANTITATIVE PROPERTIES OF BURGO CHICKEN, RED FOREST CHICKEN, AND NATIVE CHICKEN IN BENGKULU PROVINCE Teguh Rafian; Jakaria Jakaria; Niken Ulupi
Journal Animal Research and Applied Science Vol. 4 No. 1 (2023): Juni 2023
Publisher : Study Program of Animal Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/aras.v4i1.28507

Abstract

Pentingnya pemahaman yang baik tentang karakteristik SDGT sangat diperlukan untuk dijadikan sebagai referensi pengambilan keputusan dalam pengembangan dan program pemuliaan ternak. Penelitian ini bertujuan mengidentifikasi keragaman fenotipe sifat kuantitatif ayam burgo, ayam hutan merah, dan ayam kampung dalam upaya pelestarian dan pemanfaatan SDG ayam lokal khususnya di Bengkulu, dan menganalisis faktor-faktor peubah penentu ukuran dan bentuk ayam burgo, ayam hutan merah, dan ayam kampung. Bahan penelitian yang digunakan adalah 47 ekor ayam burgo, 9 ekor ayam hutan merah (Gallus gallus spadiceus), dan 18 ekor ayam kampung (Gallus gallus domesticus). Sampel diambil menggunakan teknik purposive sampling dan ayam yang diambil merupakan ayam yang sudah dewasa di Kabupaten Mukomuko, Kabupaten Bengkulu Utara, Kabupaten Bengkulu Tengah, Kota Bengkulu, dan Kabupaten Bengkulu Selatan, Provinsi Bengkulu. Data-data sifat kuantitatif berupa berat badan, panjang tubuh, panjang dada, lingkar dada, rentang sayap, panjang paha atas, panjang paha bawah, panjang shank, panjang jari ketiga, dan lingkar shank ayam burgo, ayam hutan merah, dan ayam kampung. Hasil uji-t menunjukkan bahwa karakteristik sifat kuantitatif ayam burgo jantan lebih mendekati karakteristik sifat kuantitatif ayam kampung jantan, sedangkan karakteristik sifat kuantitatif ayam burgo betina lebih mendekati karakteristik sifat kuantitatif ayam hutan merah betina. Berdasarkan hasil penelitian dapat disimpulkan bahwa: (1) ayam burgo dan ayam hutan merah memiliki sifat kuantitatif dengan tingkat keragaman yang rendah (monomorfik), sebaliknya dengan ayam kampung memiliki sifat kuantitatif dengan tingkat keragaman yang tinggi (polimorfik), dan (2) peubah penentu ukuran ayam burgo adalah panjang paha bawah (jantan) dan panjang jari kaki ketiga (betina), ayam hutan merah adalah panjang paha bawah (jantan) dan panjang paha atas (betina), dan ayam kampung adalah panjang shank, sedangkan peubah penentu bentuk ayam burgo adalah panjang paha bawah, ayam hutan merah adalah panjang jari kaki ketiga, dan ayam kampung adalah panjang shank.
Haplotype and phylogenetic diversity using mitochondrial 12S rRNA gene marker in Bali cattle T. Suselowati; R. R. Noor; J. Jakaria
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 3 (2023): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.3.169-181

Abstract

Bali cattle is one of Indonesia's original livestock genetic resources. The 12S rRNA can be used as a marker of genetic diversity, and until now, there has been no report on Bali cattle. The study was aimed to analyze the gene of mtDNA 12S rRNA to determine haplotype diversity and phylogenetics in Bali cattle populations of the eastern region of Indonesia. A total of 95 blood samples used consisted of three different populations which were Bali cattle, Ongole crossbred (PO) and Madura cattle as a comparison. This research was analyzed using PCR and sequencing methods. The data were analyzed using the cluster W method for estimating genetic distances, calculating diversity, and reconstructing phylogenetic trees using the MEGAX, DNAsP and Network. The genetic distance values ranged from 0.00200 to 0.01508, and the Haplotype diversity values ranged from 0.66000 to 0.91111. The nucleo-tide diversity values ranged from 0.00174 to 0.01673. There were 16 haplotypes found. The values of Gst, Nst and Fst were 0.00803, 0.07550, 0.07622; respectively. Based on the analysis, there were dif-ferences between Bali cattle from various populations, there were specific haplotypes. The 12S rRNA gene can be used as a genetic marker for diversity studies in Bali cattle and other cattle breeds although the diversity is low.
Identifikasi Keragaman SNP Gen MSTN Pada Sapi Limousin Wenny Ladhunka Nur Aliyya; Jakaria Jakaria; Ronny Rachman Noor
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.596

Abstract

Abstract Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP
Sustainable Development Goals: Optimalisasi Konservasi Penyu Melalui Peningkatan Pengetahuan Siswa Eka Haryati Yuliany; Mohamad Jakaria; Ervina Mukharomah; Sri Parwanti
Jurnal SOLMA Vol. 12 No. 2 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i2.11469

Abstract

Background: Keberadaan penyu telah lama terancam dari alam maupun kegiatan manusia yang membahayakan populasinya. Upaya pelestarian penyu dapat dilakukan melalui pendidikan dan pendekatan partisipatif pada generasi muda. Tujuan pengabdian masyarakat ini diharapkan dapat menambah pengetahuan siswa/i tentang penyu dan menumbuhkan rasa kepedulian terhadap pelestarian penyu. Metode: Peserta pengabdian masyarakat terdiri dari siswa/i kelas VII sebanyak 37 orang. Metode pengabdian kepada masyarakat yang digunakan metode partisipatif mulai dari ceramah, menonton video, diskusi dan tanya jawab. Teknik pengumpulan data dengan tes dan dokumentasi. Hasil: Hasil pengabdian masyarakat ini ditemukan peningkatan pengetahuan siswa dengan signifikan setelah dilakukan penyampaian materi dan tanya jawab. Di akhir kegiatan pengabdian masyarakat siswa/i memperoleh pengertian terhadap pentingnya menjaga lingkungan hidup dan keberadaan penyu. Kesimpulan: Optimalisasi konservasi penyu menggunakan video dapat meningkatkan pengetahuan siswa. Selanjutnya ditemukan antusias siswa/i menampilkan keinginan siswa/i dalam melestarikan penyu dalam proses tanya jawab.
Perkembangan Penelitian dan Pemetaan Topik pada Riset Sapi Bali berdasarkan Publikasi Jurnal Ilmiah dan Prosiding Abdul Rahman Saleh; Sri Rahayu; Jakaria Jakaria
Media Pustakawan Vol. 30 No. 2 (2023): Agustus
Publisher : Perpustakaan Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37014/medpus.v30i2.4877

Abstract

Animals that have the potential to produce meat that can be developed are Bali cattle. Unlike imported cattle, Bali cattle are superior because they can withstand all weather and adapt easily. Research on Bali cattle is quite a lot. However, we do not yet know the direction and status of the research. For this reason, it is necessary to conduct a study of the research that has been carried out. The research data comes from secondary data, namely scientific journal articles and proceedings articles. Data publication year is limited to June 2023. Data is tracked using Publish or Perish software. The data obtained was processed using Zotero, MS-Excel and VOSviewer software. There were 983 studies found, with the highest number of animal feed and nutrition, with 190 studies (19.31%). The most productive researcher is Jakaria (49 titles). The number of Bali cattle studies each year varies greatly with the highest number occurring in 2020 (169 studies). It is known that the authorship pattern of the Bali cattle research results article is in accordance with Lotka's law with an exponent value of 2.1 and a constant value of 66%. The researcher collaboration map and research topic map were successfully created using VOSviewer. The degree of author collaboration was 0.91. Related research including animal waste, animal welfare, animal farm and conservation can still be developed because there are not many researches in these fields.  
Keberlanjutan Pengelolaan Kawasan Konservasi Penyu di Pantai Kecamatan Paloh, Kalimantan Barat Mohamad Jakaria; Mirza Dikari Kusrini; Mohammad Mukhlis Kamal
Jurnal Ilmu Lingkungan Vol 22, No 5 (2024): September 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.5.1209-1223

Abstract

Keberadaan penting penyu dalam menjaga keseimbagan ekosistem laut dan potensi ekowisata pantai peneluran penyu perlu dijamin keberlanjutannya. Penurunan populasi penyu beberapa tahun tarakhir yang di akibatkan oleh kerusakan habitat pantai peneluran akibat abrasi pantai dan gangguan habitat penyu bertelur oleh kegiatan antropogenik telah terjadi di Kawasan Konservasi Penyu di Kecamatan Paloh, Kalimantan Barat. Agar pengelolaan Kawasan Konservasi Penyu di Kecamatan paloh dapat berlangsung secara berkelanjutan, maka perlu mengimplementasikan konsep pembangunan berkelanjutan melalui pelestarian habitat pantai peneluran penyu. Penelitian ini bertujuan untuk menganalisis status keberlanjutan pengelolaan Kawasan Konservasi Penyu di Kecamatan Paloh, Kalimantan Barat, dan menganalisis faktor-faktor penting yang mempengaruhi keberlanjutan pengelolaan Kawasan Konservasi Penyu Di Kecamatan Paloh, Kalimantan Barat. Metode analisis yang digunakan ialah Multiaspect Sustainability Analysis (MSA). Hasil yang didapatkan dalam bentuk indeks dan status keberlajutan. Hasil analisis terhadap empat dimensi menunjukan bahwa dimensi usaha konservasi sangat berkelanjutan (95.29), dimensi sosial berkelanjutan (75.17), dimensi ekonomi kurang berkelanjutan (49), dan dimensi hukum dan kelembagaan berkelanjutan (68.67). Terdapat empat atribut/faktor yang perlu segera ditangani dan perlu diintervensi karena berpengaruh sensitif terhadap peninkatan indeks dan status keberlanjutan yaitu fasilitas hatchery, sikap dan perliku masyarakat terhadap pengelolaan konservasi penyu, tingkat pendapatan masyarakat disekitar kawasan konservasi penyu serta penyuluhan oleh balai penyuluh.
Co-Authors . Aryogi . Aryogi, . . Hartati . hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. GUNAWAN A. Gunawan A. Gunawan A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Adelaide Jose Pereira Cristovao Adityo Raynaldo Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bambang Hermawan Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Budiman C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. A. Sari D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Darwati, Sri DEWI APRI ASTUTI E Andreas E Sari E. Andreas Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas EVA AMALIA PERTIWI F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti FADHIL MUHARRAM Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitra Aji Pamungkas Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. G. R. Putra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro I. Suhendro I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Khasanah, H. Kholijah Kocu, N. Komang Alit Paramitasari M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur MASKUR MASKUR Mirza Dikari Kusrini Mohamad Yamin Mohammad Mukhlis Kamal Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHAMMAD MUHSININ Muhammad Nico Ghossani MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Mulyono, Rini Herlina Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nadya Nurafifah Andriya Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putri Indah Ningtias R R Noor R Sari R. Afnan R. F. Istiqlal R. H. Mulyono R. Iis Arifiantini R. K. Rusli R. Misrianti R. Mutia R. Mutia R. Oktaviani R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor R.R. Noor Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Riska Dwi Nanda Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. H. Wijaya S. Hartono S. M. Rachmawati S. N. Karimah S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik . Sarwi, Annisa Sony Hartono Wijaya Sri Rahayu Sri Sulandari SRI SULANDARI Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Surya Kusuma Wijaya Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TAPAUL ROZI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. G. M. Bonawati Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari