Claim Missing Document
Check
Articles

Genetic Diversity Analysis and Determination of Specific Alleles of Kuantan Cattle Using Microsatellite Markers R. Misrianti; S. H. Wijaya; C. Sumantri; J. Jakaria
Tropical Animal Science Journal Vol. 45 No. 2 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.2.134

Abstract

Kuantan cattle have an important role in Riau Province, Indonesia. Identification of the genetic diversity of these cattle is important to get the basic information for breeding and conservation strategies. The aim of this research was to identify the genetic diversity of Kuantan cattle using microsatellite markers. A total of thirty-nine DNA samples from three breeds were used in this study. The polymerase chain reaction was conducted using four labeled primers of microsatellite (INRA035, ILSTS06, HEL9 and ETH225). The data were analyzed using GenAlEx 6.41, Cervus 3.0, POPTREE, and STRUCTURE Software. A total of thirty-two alleles were found from microsatellite loci. Two alleles in INRA035 locus 112 and 118 occurred as specific allele candidates for Kuantan cattle. The mean of observed heterozygosity value of the Kuantan-2 population (0.602) was higher than Kuantan-1 (0.471) but lower than Madura (0.688) and Pesisir cattle (0.625). PIC value was higher in HEL9 loci. The dendrogram showed that Kuantan cattle existed at different clusters with Pesisir and Madura cattle. This finding indicated that microsatellite markers successfully distinguished clusters of the cattle and could serve as information for conducting conservation and breeding program.
Teknik pengekangan sapi bali dan pola sonogram jaringan untuk pendugaan kualitas karkas hidup secara ultrasonografi Mokhamad Fakhrul Ulum; Dairoh Dairoh; Winni Liani Daulay; Prastya Idihastuti; Amin Wahyudiono; Jakaria Jakaria; Annang Dwijatmiko
ARSHI Veterinary Letters Vol. 5 No. 4 (2021): ARSHI Veterinary Letters - November 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.4.79-80

Abstract

Pengekangan merupakan langkah awal dan utama dalam menentukan keberhasilan untuk pemeriksaan hewan, seperti pada pencitraan ultrasonografi. Tulisan ini mendeskripsikan beberapa metode pengekangan sapi bali untuk pencitraan ultrasonografi di kandang penampungan dan pola sonogram yang didapatkan. Sapi bali jantan dewasa sebanyak 101 ekor dilakukan pendugaan kualitas karkas sebelum dipotong. Pengekangan fisik dilakukan dengan pilihan yaitu: mengikat sapi pada kandang komunal, mengikat sapi kandang dengan pembatas dinding, dan menempatkan sapi pada kandang jepit. Proses pengekangan berlangsung ±5-10 menit atau lebih lama tergantung temperamen sapi. Sedangkan proses pencitraan ultrasonografi berlangsung ±30-60 detik pada sudut pandang transversal dan longitudinal. Citra ultrasonografi punggung daerah rusuk 12-13 tampak ekogenitas lapisan jaringan penyusun berupa kulit, lemak subkutan, otot dan tulang punggung yang terbedakan dengan baik. Pola marbling pada sonogram otot longissimus dorsi sudut pandang transversal terlihat seperti ranting, sarang lebah, bercak-bercak, dan tanpa pola. Sedangkan citra pada sudut pandang longitudinal terlihat garis diagonal dan tanpa garis.
Identifikasi Keragaman SNP Gen MSTN Pada Sapi Limousin Wenny Ladhunka Nur Aliyya; Jakaria Jakaria; Ronny Rachman Noor
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.596

Abstract

Abstract Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP
Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep A. Gunawan; F. W. Pramukti; K. Listyarini; M. A. Abuzahra; J. Jakaria; C. Sumantri; I. Inounu; M. J. Uddin
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.1-9

Abstract

The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.
Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens A. Gunawan; S. Y. Basril; K. Listyarini; A. Furqon; W. Bilyaro; J. Jakaria; M. J. Uddin; C. Sumantri
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.201-210

Abstract

SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.
ANALISIS KORELASI GENETIK ANTARA BOBOT LAHIR, BOBOT SAPIH, DAN BOBOT YEARLING PADA SAPI BRAHMAN CROSS (BX): Genetic Correlation Analysis between Birth Weight, Weaning Weight, and Yearling Weight of Brahman Cross (BX) Cattle Fariz Am Kurniawan; Jakaria; Rudy Priyanto
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 12 No. Khusus (2022): Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian, Vol
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jstsv.12.Khusus.148-153

Abstract

Genetic correlations between productive traits have important role in beef cattle selection. This study aimed to estimate genetic correlation between birth, weaning, and yearling weight of Brahman Cross (BX) cattle. The experiment was carried out at PT Lembu Jantan Perkasa (PT LJP) Serang, Banten. There were 31 heads of BX offspring, which were obtained from 3 sire and 31 dams. The average birth weight was 23.1 ± 2.21 Kg, weaning weight 73.5 ± 13.02 Kg, and yearling weight was 257.9 ± 61.15 Kg. Genetic correlation was analyzed by covariance analysis. The genetic correlation between birth weight and weaning weight was 0.12, birth weight and yearling weight 0.31, and weaning weight and yearling weight was 0.47. The results indicated that the selection based on birth and weaning weight could be conducted in order to increase yearling weight in BX cattle, since these variables have high and positive genetic correlation to yearling weight. ABSTRAKKorelasi genetik diantara sifat-sifat produksi memiliki peranan penting dalam kegiatan seleksi sapi pedaging. Penelitian ini bertujuan untuk menduga nilai korelasi genetik antara bobot lahir, sapih, dan bobot yearling pada Sapi Brahman Cross (BX). Penelitian dilaksanakan di PT Lembu Jantan Perkasa, Serang Banten. Materi penelitian meliputi 31 ekor sapi BX yang berasal dari 3 pejantan dan 31 ekor induk betina. Rataan bobot lahir yang didapatkan sebesar 23,1 ± 2,21 Kg, bobot sapih sebesar 73,5 ± 13,02 Kg, dan bobot yearling sebesar 257,9 ± 61,15 Kg. Korelasi genetik dianalisis dengan analisis peragam antar saudara tiri. Nilai korelasi genetik antara bobot lahir dengan sapih sebesar 0,12, bobot lahir dengan bobot yearling sebesar 0,31, sedangkan bobot sapih dan bobot yearling sebesar 0,47. Hasil menunjukan bahwa pelaksanaan seleksi dapat dilakukan berdasarkan bobot lahir dan bobot sapih dalam rangka peningkatan peforma sapi BX, dikarenakan kedua peubah memiliki korelasi genetik yang positif dan tinggi terhadap bobot yearling.
Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle Adefia Fahira; Ronny Rachman Noor; Jakaria Jakaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 3 (2022): September 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i3.3045

Abstract

PLAG1 gene is one of those that regulate growth and body size.  This study aimedto look at the PLAG1 gene polymorphism and its relationship to birth weight in Bali cattle using PCR-RFLP. The total sample used was 104 samples consisting of 66 Bali cattle from BPTU-HPT Denpasar and 38 Bali cattle from BPT-HMT Serading, each of which had birth weight data. PLAG1 gene polymorphism was analyzed using PCR-RFLP and the Sac1 restriction enzyme. The genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were all examined using Popgen32. General Linear Model was used to analyze the association of SNP 795AG PLAG1 gene with birth weight in Bali cattle. Amplification of the PLAG1 gene resulted in 776 bp fragments and two alleles. The PLAG1 gene had three genotypes: AA (562 bp and 182 bp), AG (562 bp, 182 bp, and 104 bp), and GG (562 bp, 182 bp, and 104 bp). Based on the results, the PLAG1 gene in Bali cattle was polymorphic. The alleles frequency of Bali cattle wasin Hardy-Weinberg equilibrium. The SNP c.795AG PLAG1 gene genotype were associated with birth weight in Bali cattle. The A allele is a determinant of high birth weight in Bali cattle where the AG genotype has the highest birth weight.
Determination of Complete Sequence Mutation of Myostatin Gene in Fast- and Slow-Growing Chicken Isyana Khaerunnisa; Ahmad Furqon; Saiful Anwar; Jakaria Jakaria; Cahyo Budiman; Irma Isnafia Arief; Cece Sumantri; Yong Soo Kim
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.148-158

Abstract

Myostatin plays a role in inhibiting skeletal muscle growth in vertebrates. This study aimed to investigate the full sequence of the myostatin gene in fast-growing and slow-growing chickens. Fast- and slow-growing chicken models were produced from F2 Kampung x broiler. The full sequence of the myostatin gene was identified using 24 pairs of primers covering about 8,000 bp. mRNA expression analysis of muscle tissue was performed to examine whether the expression levels of myostatin are affected by chicken lines, sex, or muscle type. The results showed 170 mutations in fast- and slow-growing chickens. One hundred and sixty-one of them are novel mutations. A total of five and twenty-two alleles were specific alleles found only in the fast-growing and slow-growing groups of chickens, respectively. There were no differences in amino acids and gene expression levels of myostatin between the fast- and slow-growing chickens. In summary, the results of this study showed that specific alleles for the fast-growing or slow-growing chicken groups were found, suggesting that these specific alleles potentially be used as genetic markers for muscle growth in chickens.
The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance I. Suhendro; J. Jakaria; R. Priyanto; W. Manalu; R. R. Noor
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.429

Abstract

Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T>G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T>G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp & 498 bp; GG with 144, 236, & 262 bp; and TG with 144, 236, 262, & 498 bp. Bali cattle with the GG genotype had lower (p<0.001) Rr and HTC compared to the other genotypes. It could be concluded that physiological performances were lower at high altitudes, and the SNP -69T>G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T>G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.
DIVERSITAS GENETIK DAN HAPLOGROUP KAMBING GEMBRONG BERSTATUS KRITIS DI KABUPATEN KARANGASEM, BALI (Genetic Diversity and Haplogroup of Endangered Gembrong Goat In Karangasem, Bali) M. Syamsul Arifin Zein; Sri Sulandari; Jakaria Jakaria; I Made Londra; Suprio Guntoro; Ida Bagus Gaga Partama
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.941 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5134

Abstract

This study was conducted to evaluate the genetic diversity and phylogeny of Gembrong goat. For this purpose, 21 goats from endangered breed in Karangasem Bali were used. Molecular analysis of genetic diversity and phylogeography used hypervariable segment 1 of mitochondrial DNA control region. The result showed that genetic variability of Gembrong goat was homogeneous with only one different sites, namely the substitution pyrimidines of C ↔ T (transitional). Phylogeny analysis results showed maternal origin of Gembrong goat is lineage (subhaplogroup) B1 with frequency of 100%. Haplogroup B were known has been domesticated from wild goat in western Asia, then headed to south Asia and infiltrated to southeast Asia, including Gembrong goat in Bali, Indonesia. As a conclusion, genetic diversity of Gembrong goat from remaining population in Karangasem very low and originate from lineages/haplogroup B1 with a frequency of 100%.
Co-Authors . Aryogi . Aryogi, . . hartati . Hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. Gunawan A. Gunawan A. GUNAWAN A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Abdul Rahman Saleh Acep R Jayaprawira Achmad Fariz Adefia Fahira Ahmad Furqon alim, iqbal Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Anggreini, Heny Annang Dwijatmiko Asep Gunawan Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bonawati, Y. G. M. Bramada Winiar Putra Budiman, C. C Sumantri C. Budiman C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. SUMANTRI C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Dairoh, D. DEWI APRI ASTUTI E Andreas E Sari E. Andreas E. Handiwirawan Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah Eryk Andreas F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti Fariz Am Kurniawan Fauzan Habibi Lubis Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Ghossani, Muhammad Nico Gunawan Sitanggang H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hikmawaty, Hikmawaty Himmatul Khasanah I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro Ida Bagus Gaga Partama Ila Rosmilawati Irma Isnafia Arief Ishak, Andi Baso Lompengeng Istiqlal, R. F. Isyana Khaerunnisa Isyana Khaerunnisa Jessy Mainidar K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kania Larisa Dwiyani Karimah, S. N. Khasanah, H. Kholijah Kholijah, K. Kocu, N. Komang Alit Paramitasari Listyarini, K. Londra, I. M. M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Mahmud, Rois Mahmudi Mahmudi Maria Ulfah Maryani Maryani Maskur Maskur Mohamad Yamin Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi MUHARRINA HARAHAP, MUHARRINA Mulyono, R. H. Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Ni Made Paramita Setyani Niken Ulupi Nur Faiz Habibah P. P. Agung, P. P. Pertiwi, E. A. Prastya Idihastuti Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putra, I. G. R. Putri Indah Ningtias R R Noor R Sari R. Afnan R. H. Mulyono R. Iis Arifiantini R. Ismail R. K. Rusli R. Misrianti R. Mutia R. Mutia R. OKTAVIANI R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor Rachmawati, S. M. Restu Misrianti Reza, Muhammad Aulia Richard Wijaya Putra Rini Fitriani Rini Herlina Mulyono Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rusli, Ridho Kurniawan S S Mansjoer S. Darwati S. Elieser S. H. Wijaya S. Hartono S. Rahayu, S. S. Subandriyo S. Suharti S. Sulandari S. Y. Basril Saiful Anwar Salundik Saputra, E. A. Sari, D. A. Sarwi, Annisa Sony Hartono Wijaya Sri Darwati Sri Rahayu SRI SULANDARI Sri Sulandari Sri Wahyuni Siswanti Stevie Claudya Putri Nober Suganda Suhendro, I. Suhendro, Ikhsan Suparman, A. B. P. Suprio Guntoro Suprio Guntoro Sutikno Madnasri Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syakif Ahmad Syamsul Arifin T I Amalianingsih T Suryati T. Rafian T. Suselowati T. Toharmat TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Setyaudin Teguh Rafian Toto Toharmat Tya I Amalianingsih Ulum, M. F. W. Manalu W. Manalu Wasmen Manalu Wenny Ladhunka Nur Aliyya Wike andre Septian Winni Liani Daulay Winni Liani Daulay Wiwit Yuliesie Rasyid Woki Bilyaro Y. Parwoto Yong Soo Kim Yureni Sahril Dedi Z Wulandari