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HISTOLOGI PERKEMBANGAN ORGAN LARVA YELLOWFIN TUNA (Thunnus albacares) Yuliana Asri; Kukuh Nirmala; Tri Heru Prihadi; John Harianto Hutapea; Muh Azril
Jurnal Perikanan Unram Vol 13 No 4 (2023): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v13i4.717

Abstract

Yellowfin tuna merupakan komoditas perikanan yang memiliki nilai ekonomis tinggi. Kendala utama dalam budidaya yaitu terjadi kematian massal pada stadia awal pemeliharaan larva. Pengamatan terkait perkembangan awal organ larva yellowfin tuna secara histologi perlu dilakukan untuk mendapat informasi dasar dalam pengembangan teknik pemeliharaan larva. Tujuan dari penelitian ini adalah untuk mengetahui perkembangan organ penglihatan, penciuman, gelembung renang dan sistem pencernaan larva secara histologi. Sampel larva diambil sebanyak 15 ekor pada umur satu hari (D1), D3, D5, D7, D10 dan D13. Metode histologi yang digunakan adalah metode Bouins. Sampel direndam selama 4–6 jam pada larutan bouins, dipindahkan ke dalam larutan berisi alkohol 70% untuk didehidrasi dengan konsentrasi bertingkat hingga 100%. Sampel dijernihkan dalam xylene dan diresapkan dalam paraffin, dipotong dengan microtom dan pewarnaan dengan haematoxylin dan eosin. Hasil menunjukkan pada D1 sistem pencernaan berbentuk tabung lurus sederhana, sistem penglihatan, penciuman dan gelembung renang belum terbentuk sempurna. Hari ke 3 saluran pencernaan mulai terbentuk, organ telinga dalam, penciuman, mata, esofagus, lambung dan ginjal juga sudah mulai terbentuk. Retina dan pigmen mata sudah berkembang lebih baik, gelembung renang juga sudah terbentuk dan organ penciuman semakin jelas pada umur D5. Organ-organ larva terus berkembang seiring dengan pertumbuhan dan pertambahan umur larva. Berdasarkan perkembangan penglihatan, penciuman, pencernaan larva yellowfin tuna sudah bisa diberikan pakan pertama pada hari ke 3 setelah menetas.
Monitoring the water quality of Belawan Sea for rearing asian seabass Lates calcarifer in a floating net cage system Iman Sari Lubis, Vina; Nirmala, Kukuh; Supriyono, Eddy; Hastuti, Yuni Puji
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.269-280

Abstract

Belawan is a coastal area located in Medan Belawan District, North Sumatra Province. The aim of the research is to determine the feasibility status of the study location if it is developed as a location for floating net cages (KJA). The research was carried out in the sea waters of Belawan in August–September 2022. The research parameters observed during the research were hydrooceanographic data and water quality data including physical parameters, chemical parameters, biological parameters. Determination of water quality status using the STORET Index, CCME WQI Index and principal component analysis (PCA). Observation parameters during the research were clinical symptoms, histological observations and growth performance of Asian sea bass. The research results show that tides, COD and turbidity are parameters that do not comply with water quality standards. The types of phytoplankton consist of 15 species and 4 classes, namely Cyanophyceae, Chlorophyceae, Bacyllariophyceae and Dynophyceae. Observation total vibrio count (TVC) shows that in all water samples it is Log 105 CFU/mL and in snapper organs (gills, liver, kidneys) there are Log 104-105 CFU/g. The results of the research can be concluded that for each zone in Belawan waters there is no difference in the level of pollution between zones in terms of the STORET index (moderately polluted) and the CCME WQI index (marginal) with limiting factors namely COD and turbidity. Belawan waters are in less than suitable condition in August–September for snapper cultivation activities in floating net cages (KJA) characterized by low SR and ADG values. Keywords: Belawan Waters, CCME WQI index, STORET index, principal component analysis (PCA), water quality monitoring ABSTRAK Belawan termasuk kawasan pesisir yang terletak di Kecamatan Medan Belawan, Provinsi Sumatera Utara. Tujuan dari penelitian adalah untuk menentukan status kelayakan lokasi kajian jika dikembangkan sebagai lokasi keramba jaring apung (KJA). Penelitian dilaksanakan di perairan laut Belawan, Kota Medan yaitu pada bulan Agustus–September 2022. Metode pengambilan sampel dilakukan secara purposive sampling. Adapun parameter penelitian yang diamati selama penelitian yaitu data hidro oseanografi dan data kualitas air meliputi parameter fisika (konduktivitas, TDS, salinitas, suhu, kecerahan, kekeruhan), parameter kimia (DO, BOD, COD, amonia, pH), parameter biologi (plankton, TVC). Penilaian kualitas perairan menggunakan Indeks STORET, Indeks CCME WQI dan principal component analysis (PCA). Parameter pengamatan saat penelitian yaitu gejala klinis, pengamatan histologi dan kinerja pertumbuhan ikan kakap putih. Hasil penelitian menunjukkan bahwa pasang surut, COD dan kekeruhan merupakan parameter yang tidak sesuai dengan baku mutu kualitas air. Adapun jenis fitoplankton terdiri dari 15 spesies dan 4 kelas yaitu Cyanophyceae, Chlorophyceae, Bacyllariophyceae dan Dynophyceae. Pengamatan total vibrio count (TVC) menunjukkan bahwa pada seluruh sampel air yaitu Log 105 CFU/mL dan pada organ ikan kakap (insang, hati, ginjal) yaitu Log 104-105 CFU/g. Hasil penelitian dapat disimpulkan bahwa setiap zona dalam perairan Belawan tergolong tercemar sedang dan tidak terdapat perbedaan tingkat pencemaran ditinjau dari indeks STORET dan indeks CCME WQI dengan faktor pembatas yaitu COD dan kekeruhan. Berdasarkan hal tersebut, Perairan Belawan dalam kondisi kurang layak untuk kegiatan budidaya ikan kakap di keramba jaring apung (KJA) ditandai dengan nilai SR dan ADG yang rendah. Kata Kunci: indeks CCME WQI, indeks STORET, monitoring kualitas air, Perairan Belawan, principal component analysis (PCA)
Effect of Light Intensity on Ammonium Removal and Biomass Growth in Different Levels of Aquaculture Effluent Using Duckweed (Lemna perpusilla) Agus Waluyo; Nirmala, Kukuh; Satya, Awalina; Puji Hastuti, Yuni; Chrismadha, Tjandra; Susanti, Evi; Sumi Lestari, Fajar; Nafisyah, Eva; Sugiarti; Nasrul, Muit
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 30 No. 2 (2024)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2024.6420

Abstract

Cultivating duckweed in aquaculture effluent offers a viable approach to eliminating contaminants. The duckweed biomass obtained can be utilized for the generation of bioenergy. However, elevated levels of ammonium (NH4+) in aquaculture effluent, combined with variations in light intensity, can hinder biomass formation. The precise mechanisms underlying this inhibition remain incompletely elucidated. The study assessed the efficacy of duckweed (Lemna perpusilla) as a treatment agent for wastewater from catfish farms. The objective was to evaluate the growth response of duckweed and its efficacy in reducing ammonium levels. The research demonstrated that daily light intensity fluctuated using shade nets and that the ammonium concentration of aquaculture wastewater varied according to the age of the fish. The shade nets, which blocked 25% of the sunlight and had an average daily light intensity of 3433.34–15199.56 lux, demonstrated a slightly elevated NH4+ removal efficiency and duckweed productivity of 69.34% and 0.050 kg/m²/day, respectively. However, these values were not statistically significant when compared to conditions without shade nets, which had a removal efficiency of 63.97% and duckweed productivity of 0.042kg/m2/day (P<0.05). The implementation of shade structures that effectively decrease solar exposure by 25% shows promise for enhancing duckweed productivity and optimizing nutrient reduction in wastewater from fish cultivation systems. This approach contributes to the promotion of sustainable integrated aquaculture.
The Role of Bakau Snail, Telescopium telescopium L., as Biofilter in Waste Water Management of Intensive Shrimp Culture Hamsiah, ,; Djokosetiyanto, Daniel; Adiwilaga, E. M.; Nirmala, Kukuh
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.587 KB) | DOI: 10.19027/jai.1.57-64

Abstract

ABSTRACTThe objective the experiment is to know the role of bakau snail, Telescopium telescopium L., as biofilter for improving waste water quality in shrimp culture.  The experiment was carried out at laboratory scale.  The parameters that observed in this experiment are physical, chemical and biological of waste water.  Growth and survival rate of snail were also observed. Waste water quality measurement was carried out during a week, while the growth and survival rate were measured during two months.  The aquarium of 30x40x40 cm were filled with 30 l of waste water from intensive shrimp culture.  Bakau snail were stocked to the aquarium with density of 0 (control), 6, 9 and 12 snail/aquarium, and these treatment were replicated 3 times.  The result shown that total organic matter (TOM), total ammonia, dissolved oxygen (DO) of waste water, and growth and survival rate of snail were not different between treatment of stocking density, while the biological oxygen demand (BOD), total suspended solid (TSS), nitrite and nitrate were significantly different (pKey words :  Bakau snail, Telescopium telescopium L.,  biofilter, shrimp culture waste water. ABSTRAKPercobaan ini bertujuan untuk mengkaji peranan keong bakau, Telescopium telescopium L., sebagai biofilter terhadap perbaikan mutu air limbah budidaya tambak udang intesif.  Pertumbuhan dan kelangsungan hidup keong bakau juga dikaji.  Percobaan dilakukan dalam skala laboratorium.  Pengamatan kualitas air fisika, kimia dan biologi air limbah budidaya tambak dilakukan selama seminggu, sedangkan pertumbuhan keong dilakukan selama 2 bulan.  Wadah percobaan yang digunakan adalah akuarium ukuran 30x40x40 cm dan diisi air sebanyak 30 liter yang berasal dari buangan budidaya udang intensif di tambak.  Perlakuan percoban berupa padat tebar keong bakau dalam akuarium yaitu: 0 (tanpa keong), 6, 9 dan 13 ekor/akuarium, dan setiap perlakuan diulang 3 kali. Hasil percobaan menunjukkan bahwa respon peubah kadar bahan organik total (TOM), amoniak total, oksigen terlarut (DO) dalam air limbah, serta pertumbuhan dan kelangsungan hidup keong bakau tidak berbeda antar perlakuan kepadatan, sedangkan BOD5, padatan tersuspensi total (TSS), nitrit dan nitrat berbeda nyata (pKata kunci :  Keong bakau, Telescopium telescopium L., biofilter, air limbah budidaya udang.
Productivity and Chemical Parameters in the Bottom Soil of 1 and 3 years operated pond of Black Tiger Prawn Penaeus monodon Fab. Culture Nirmala, Kukuh; Yuniar, E.; Budiardi, T.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.721 KB) | DOI: 10.19027/jai.4.5-11

Abstract

This study was carried out to observe the productivity and bottom soil chemical aspects of 1 year  and 3 years operated pond of black tiger prawn Penaeus monodon Fab. culture. The results of study depicted that pond operated for 3 years contained higher (P
Effect of Hardness in 3 ppt of Water Salinity on growth and survival rates of Barb Fish (Barbus conhonius Hamilton-Buchanan) Nirmala, Kukuh; Wulandari, R.; Djokosetiyanto, D.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.63 KB) | DOI: 10.19027/jai.4.17-24

Abstract

This study was conducted to determine the effect of hardness on growth and survival rate of barb fish (Barbus conchonius Hamilton-Buchanan) reared in the 3 ppt of water salinity.  Fish were reared in three different hardnesses, i.e., 54, 72, and 90 mg/L CaCO3 for 30 days.  The results of study showed that fish reared in water hardness of 72 mg/L CaCO3 had higher daily growth rate (1.15%) compared with other treatments.  Daily growth rate of fish reared in water hardness of 54 and 90 mg/L CaCO3 were 0.76 and 0.37%, respectively.  There was no effect of hardness on survival rate of barb fish. Keywords: barb fish, Barbus conchonius,  hardness, salinity   ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh kesadahan yang berbeda terhadap laju pertumbuhan dan tingkat kelangsungan hidup ikan barbir (Barbus conchonius Hamilton-Buchanan) yang dipelihara pada air bersalinitas 3 ppt.  Ikan dipelihara dalam 3 tingkat kesadahan yang berbeda, yaitu 54 mg/L CaCO3, 72 mg/L CaCO3, dan 90 mg/L CaCO3 selama 30 hari.  Hasil penelitian menunjukkan bahwa laju perumbuhan harian tertinggi diperoleh pada ikan yang dipelihara pada kesadahan 72 mg/L CaCO3 (1,15%) dibandingkan dengan perlakuan lainnya. Laju pertumbuhan harian ikan yang dipelihara pada kesadahan 54 dan  90 mg/L CaCO3 masing-masing mencapai 0,76% dan 0,37%.  Kelangsungan hidup ikan barb tidak dipengaruhi oleh kesadahan. Kata kunci: ikan barbir, Barbus conchonius, kesadahan, salinitas
The growth performance of Osphronemus goramy reared in saline water with electrical field exposure Nirmala, Kukuh; Rasmawan, .
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.392 KB) | DOI: 10.19027/jai.9.46-55

Abstract

The aim of this study was to know the optimal salinity level on growth rates of giant gouramy Osphronemus goramy  reared in different salinity with electrical field exposure. Four different salinities tested were 0; 3; 6 and 9‰ with the electrical field exposure of 10 Volt. The experiment design was arranged in completely randoumizes design with four treatments and three replications. Stock density was 3 fish/l with mean initial total body length of 7.18±0.30 cm and initial body weight of 5.68±0.67 g. Result of study showed that the treatment of 3‰ shows the best growth performance with specific growth rates of 1.02±0.10% and growth of absolute length of 0.56±0.18 cm. Key words: Salinity, electrical field, growth rate, Osphronemus goramy   ABSTRAK Tujuan penelitian ini adalah untuk mengetahui salinitas optimal untuk pertumbuhan ikan gurame Osphronemus goramy yang dipelihara pada media bersalinitas berbeda dengan paparan medan listrik. Perlakuan meliputi empat salinitas media yang berbeda: 0, 3, 6, dan 9‰ dengan paparan medan listrik 10 Volt. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan dan 3 ulangan. Padat penebaran ikan adalah 3 ekor/l dengan rata-rata panjang total 7,18±0,30 cm dan bobot rata-rata awal 5,68±0,67 g. Hasil penelitian menunjukkan bahwa pertumbuhan ikan gurame terbaik pada penelitian ini dicapai pada salinitas 3‰ dengan pertumbuhan bobot 1,02±0,10% dan pertumbuhan panjang mutlak 0,56±0,18 cm.   Kata-kata kunci: Salinitas, medan listrik, laju pertumbuhan, Osphronemus goramy
Toxicity of mercury (Hg) on survival and growth rate, hemato- and histopathological parameters ofOreochromis niloticus Nirmala, Kukuh; Hastuti, Yuni Puji; Yuniar, Vika
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1170.716 KB) | DOI: 10.19027/jai.11.38-48

Abstract

Heavy metals are serious pollutants of the aquatic environment because of their environmental persistence and ability to be accumulated by aquatic organisms. Oreochromis niloticus exposed to 0, 0.16, 0.5, and 1.0 ppm Hg for 30 days. The aim of this study was to determine the influence of mercury in water on survival rate, growth rates, hematological, and histological parameters of Oreochromis niloticus. This study was conducted from Mei to June 2009. The experimental design was arranged in completely randomized design with four treatments and three replications. Stock density was 8 fish/aquarium with mean initial body weight was 15.70±1.13 g. Growth and survival rates of test fish were decreased with increasing the Hg concentration. Red blood cell (RBC) count, haematocrit content, and haemoglobin content decreased when compared to the control. The number of white blood cells (WBC) increased in mercuric treated fish. The results are statistically significant at p<0.05 level. Keywords:mercury, survival and growth rate, hematology, histopathology, Oreochromis niloticus
Nitrogen and phosphorus absorption capability in environmental culture by Taiwan gravestone Anadonta woodiana Lea Hastuti, Yuni Puji; Nirmala, Kukuh; Setioaji, Tyas
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1165.056 KB) | DOI: 10.19027/jai.11.86-95

Abstract

Nitrogen and phosphorus in a water culture are derived from the metabolism of the farmed organism, feed residue and microbial activity in the water. One way of controlling water quality due to waste in the form of nitrogen and phosphorus from aquaculture is to utilize filter feeder organisms of Anadonta woodiana Lea. This study aimed to analysis the effectiveness of the absorption of nitrogen and phosphorus in waters by A. woodiana as filter feeder. A total of 0 (control), five, ten, and 15 gravestone were putted into a tank of 35 L of water derived from Cirata reservoir. Each treatment was given three times repetition. Measurement of total nitrogen and phosphorus in gravestones were performed at baseline and end of treatment. In addition, measurement of total nitrogen and phosphorus in the water tank was measured every ten days. Another measurement is the length and weight of gravestone. Direct observations made on gravestones barnacle survival, pH, dissolved oxygen, temperature, and turbidity every day. Aeration was provided in each aquarium. Based on the results obtained, the survival of gravestone in the density of five gravestones was 93.33%, in ten gravestones density was 90%, while in 15 gravestones was 95%. The percentage reduction in total nitrogen in the water at the end of treatment was 57% for the five gravestones density, and for ten gravestones was 88.2%. The decrease of total phosphorus in water in ten gravestones density was 37.4%, whereas the increase in density of 15 gravestones and total phosphorus water percentage was 26.9%. On the density of ten gravestones, the body weight decreased until to 3.3%, while in the density of 15 gravestones decreased until to 2.5% on day 30. Results of water quality analysis showed that the DO values decreased from baseline to end of treatment (from 5.08 to 4.86 mg/L), the average water temperature from 26.5 to 27.4 °C, pH values 7‒8, and highest turbidity values was obtained in the density of five gravestone. Decrease in the percentage of total nitrogen in the water suggested nitrogen and phosphorus have been absorbted for barnacle growth. Keywords: total phosphorus, total nitrogen,waste,Anadonta woodiana Lea,cultivation
The addition of salt in the water media containing zeolite and active charcoal on closed system transportation of gourami fish fry Osphronemus goramy Lac. Nirmala, Kukuh; Hadiroseyani, Yani; Widiasto, Riza Purbo
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1321.792 KB) | DOI: 10.19027/jai.11.190-201

Abstract

Transportation of fish fry with high density in closed system will reduce levels of O2, increasing CO2 and NH3, will also elevate the fish stress so that increase fish mortality. To reduce the effects of increased CO2 and NH3 can be applied by using zeolite and activated charcoal, while to reduce the fish stress is through the addition of salt. This study aims to determine the dose of salt added into the water containing zeolite and activated charcoal in a closed transportation system with a high fry density for 72 hours. The study was conducted two stages, namely the preliminary study and the primary study. The preliminary study involved the observation of the survival rate of fish fry during fasting, oxygen consumption rate of fish fry, the rate of total ammonia nitrogen (TAN) excretion of fish fry, and the adsorption capacity of TAN by zeolite and activated charcoal. In the primary study, fry transport simulations was carried out for 72 hours in the laboratory. Gourami fry (body length of 4 cm and body weight of 1.7 g) with the fry density of 50 fish/L were placed in the packing bag which has been filled with zeolite as much as 20 g/L and activated charcoal as much as 10 g/L. The study used a completely randomized design with five treatments and two replications: A: blank (without zeolite, activated charcoal, and salt), B: control (20 g/L zeolite+10 g/L activated charcoal), C: 20 g/L zeolite+10 g/L activated charcoal and 1 g/L salt, D: 20 g/L zeolite+10 g/L activated charcoal and 3 g/L of salt, and E: 20 g/ L zeolite+10 g/L activated charcoal and 5 g/L salt. The results of preliminary study showed that the survival rate of fish fry was 100% and active swimming for five days without food, the level of oxygen consumption as much as 1340.28 mgO2, produce NH3 as much as 22.64 mg/L, while zeolite and activated charcoal adsorbs >50% of TAN in time of 120 seconds. In the primary study, the survival rate of fish fry during the 72-hour transportation for treatments A, B, C, D, and E, were 0%, 57%, 59%, 65% and 74%, respectively. Treatment E with survival rate of 74% was obtained at NH3 concentration of 0.016±0.069 mg/L and CO2 as high as 89.89 mg/L while the DO of 4.26 mg/L. The highest growth and survival rate of fry for 17 days post-transportation was treatment E, accounting to 2.95% and 100%, respectively.Keywords: zeolite, active charcoal, closed system transportation, survival rate, gourami fish
Co-Authors . Rasmawan Adianto, Asep Agus Priyadi Agus Waluyo Ahmad Habibie, Ahmad Alexander Burhani Marda Ali Aminulloh Alimuddin Anang Hari Kristanto Ani Widiyati Ani Widiyati Ani Widiyati Ani Widiyati Anwar, Rifky Alwafi Any Widiyati Apriana Vinasyiam Aras, Annisa Khairani Arfan Afandi Arif Faisal Siburian Arlina Ratnasari Asep Rachmat Pratama Asri, Yuliana Astari, Belinda Atma Jaya Salman Muin Aziz, Haikal Azril, Muh Berlianti . Berry Juliandi Bianingrum Bianingrum Cecep Kusmana Chandra Yudistira, Chandra Chrismadha, Tjandra D. Djokosetiyanto D. Djokosetyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEDI JUSADI Dewi Puspaningsih Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Islama Dodi Hermawan E. M. Adiwilaga E. Yuniar Eddy Supriyono Enang Haris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja, Enang Harris Erlania Erlania Erna Yuniarsih Ernik Yuliana Eva Prasetiyono Evi Susanti Farah Diana Farida Farida farida farida Febrina Amalia Fernando Jongguran Simanjuntak Gunawan, Bambang Kusmayadi Hamim Hamim Hamsiah, , Hamzah, Aris Sando Haris luthfi Harton Arfah Hastiadi Hasan Henry Kasmanhadi Saputra Humairani, Humairani Hutagaol, Manuntun Paruliah Hutapea, John Harianto I Nyoman Radiarta Idil Ardi Iis Diatin Ilyas, Anita Prihatini Imam Taufik Iman Rusmana Iman Sari Lubis, Vina Imron Imron Imron Imron, Imron Irzal Effendi Izhar Amirul Haq Jannesa Nasmi John Harianto Hutapea Joni Haryadi Julie Ekasari Kukuh Adiyana Kukuh Adiyana Kuntari, Wahyu Budi Kurnia Faturochman, Kurnia Kurnia Faturrohman Lilis Nurjanah M. Faisol Riza Ghozali M. Zairin Junior Melati, Aulia Firda Mia Setiawati Mochamad Nurdin Moh. Burhanuddin Mahmud Mohammad Nurdin Muh Azril Muhamad Yamin Muhammad Amien H Muhammad Ammar Muhammad Firmawan Muhammad Hanif Azhar Muhammad Subhan Hamka Muhammad Zairin Jr. Munawar Khalil MUNTI YUHANA Nafisyah, Eva Nasrul, Muit Novi Susianti Nur Bambang Priyo Utomo Nur Fauziyah Nuradzani, Daffa Nurhidayat Nurhidayat Nurul Taufiqu Rochman Nurul Taufiqu Taufiqu Rochman Petrus Rani Pong-Masak Prama, Ega Aditya Pratama, Asep Rachmat Priyo Handoyo Wicaksono Purnamawati, , Puspaningsih, Dewi Putri, Shofii Amaliah Qorie Astria R. Wulandari Rahmadi Azis, Rahmadi Retnosari Retnosari Revfvi Al Ghaney Rizal Riani Rahmawati Ridwan Affandi RIDWAN AFFANDI Rina Hesti Utami Riri Ezraneti Riri Ezraneti Ris Dewi Novita Riza Purbo Widiasto Rizkiya, Iffi Rizky Armansyah Robin Robin Robin, , Ruku Ratu Borut Ruspindo Syahputra Sabilu, Kadir Sabilu, Murni Sandra Sukmaning Adji Santosa Koesoemadinata Satya, Awalina Setyo Pertiwi Sheny Permatasari Sri Nuryati Sri Nuryati Sri Wahyuni Firman Sudarto Sudarto Sugeng Budiharsono Sugiarti Suhaiba Djai Suharyanto Sukenda, Sukenda, Sumi Lestari, Fajar Syarif Budiman T. Budiardi Tanjung, Tanjung Tatag Budiardi Tri Heru Prihadi Tri Heru Prihadi Tri Widiyanto Tridesianti, Siska Triheru Prihadi, Triheru Tyas Setioaji Vika Yuniar Wa Iba, Wa Iba Wahyu Wahyu Wardani, Sulistia WIDANARNI WIDANARNI Widiatmaka Widiatmaka widiatmaka Widiyati, Any Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam, Wildan Wildan Nurussalam Wirantari, Ayu Puspa Wisriati Lasima Wiwin Ambarwulan Wiyoto Wiyoto Y. Hadiroseyani yogi Himawan Yogi Himawan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yuli Siti Fatma Yuli Siti Fatma Yuliana Asri Yuni Puji Hastuti Zulfana Fikru Sifa