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All Journal Media Kedokteran Hewan Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Andrology and Biomedical Journal Jurnal Kedokteran Hewan Smart Dedication: Jurnal Pengabdian Masyarakat

Suherni Susilowati

Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya 60115

Author-ID : 3693314

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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PENGARUH BAHAN PENGENCER SARI KACANG KEDELAI (Glycine max) TERHADAP VIABILITAS DAN NEKROSIS SPERMATOZOA DOMBA SAPUDI Immelda, Kicky Hanis; Susilowati, Suherni; Yudaniayanti, Ira Sari
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of study was to find out the effect of different concentrations of soya bean extractin sperm diluter, stored at 5°C toward sperm viability and necrosis. The semen was divided into four groups : P0: 0.1 ml sapudi ram + 1 ml of diluent yolk citrate, P1: sapudi ram 0.1 ml + 1 ml diluent soybeans extract konsentration 5%, P2: 0.1 ml sapudi ram + 1 ml diluent soybeans extract konsentration 10%, P3: 0.1 ml sheep semen + 1 ml diluent soybeans konsentration 15%. The result showed that the dose of soya bean extract diluter konsentration of 15% could increase the viability percentage and could decrease the necrosis percentage of sperm.

PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Arini, Mirza Asafaizh; Suprayogi, Tri Wahyu; Warsito, Sunaryo Hadi; Sardjito, Trilas; Susilowati, Suherni; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 Î¼g / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5Î¼g / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 Î¼g / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Vladika, Maulinda Agchirilia; Warsito, Sunaryo Hadi; Susilowati, Suherni; Hernawati, Tatik; Kurnijasanti, Rochmah; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Swari, Wahyu Retno; Sabdoningrum, Emy Koestanti; Wurlina, Wurlina; Susilowati, Suherni; Kurnijasanti, Rochmah; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Khoirunnisa, Itrah; Susilowati, Suherni; Maslachah, Lilik; Suprayogi, Tri Wahyu; Kurnijasanti, Rochmah; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.

PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Cesaria, Mayrena Ayu; Estoepangestie, A.T.Soelih; Susilowati, Suherni; Hernawati, Tatik; Madyawati, Sri Pantja; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan's Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Azura, Sarah; Ratnani, Hermin; Susilowati, Suherni; Hariadi, Mas'ud; Samik, Abdul; Soepranianondo, Koesnoto
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

Pemberian silase, complete feed, dan growth promoter pada sapi perah kawin berulang terhadap Services per Conception dan produksi susu Susilowati, Suherni; Wurlina, Wurlina; Mulyati, Sri; Utama, Suzanita; Meles, Dewa Ketut
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.28-34

Repeat breeding is a reproductive disorder that harms dairy farmers because of the unachieved target to obtain one calf per year and decreased milk production. The repeat breeding syndrome can be caused by malnutrition. This study aimed to determine the effect of feeding silage, complete feed, and growth promoters in repeat breeder cows to restore the services per conception (S/C) and daily milk production. Thirty repeat breeder Holstein Friesian cows were divided randomly into three groups. The control group (P0) was fed 30-40 kg forage and 3-4 kg concentrate/head/day. Group P1 was given 8-10 kg silage or complete feed alternately every day; whereas P2 was given the same as for the P1 group, added with 10 ml growth promoter/head/day. Milk production and S/C were measured after six months of treatment. The results showed that the P1 and P2 groups returned S/C to normal (1.40 ± 0.52 and 1.30 ± 0.48), while the S/C of P0 group was 3.20 ± 0.63 (p <0.05). Daily milk production in the P1 and P2 groups (14.20 ± 1.40 and 14.60 ± 1.43 l/d) was also higher (p <0.05) than the control group (8.30 ± 1.06 l/d). It could be concluded that feeding silage and complete feeds alternately daily, with or without the addition of growth promoter, improved the S/C to the normal range and increased milk production.

Efisiensi reproduksi pada sapi perah yang teridentifikasi Bakteri non spesifik dalam saluran reproduksi Qodri, Winda Nurul Lailatil; Mulyati, Sri; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Susilowati, Suherni; Tyasmingsih, Wiwiek
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.41-47

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collected from interview, questionaire, and direct observation at the beginning of the study. Detection of estrus was performed visually, followed by artificial insemination 12-18 hours later. Non-return to estrus was checked after 21 days. Artificial insemination was repeated up to three times on cows returning to estrous. Pregnancy was checked through rectal palpation 60 days after the last insemination. Pregnant cows were followed until calving. The results showed that the estrus rate, non-return rate (NRR), conception rate (CR), calving rate (CvR), services per conception (S/C), and days open (DO), were respectively 100%, 85%, 85%, 85%, 1.3, and 93 days. It could be concluded that the presence of non-specific bacteria in the reproductive tract of dairy cows did not affect the reproductive efficiency so that pregnancy and calving could still occur.

Interkorelasi antara persentase konsentrat, kadar urea nitrogen susu dan beberapa variabel sapi perah Friesian Holstein Rizal, Syaiful; Utama, Suzanita; Utomo, Budi; Susilowati, Suherni; Mulyati, Sri; Ismudiono, Ismudiono; Soeharsono, Soeharsono
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.53-59

The purpose of this study was to identify the correlation among the percentage of feed concentrate, milk urea nitrogen (MUN), milk production, services per conception (S/C), calving interval (CI), and days open (DO) of Holstein Friesian cows on smallholder farms. Twenty four, 4-6 year old, non-pregnant, lactating cows, with body condition score (BCS) 5-7, having given birth 2-5 times were recorded their daily milk production, S/C, CI, DO, and the percentage of concentrate in feed given. Milk samples were collected to measure MUN. The correlation calculation among the six variables resulted 15 correlation values, ten of which were not significantly different (p >0.05), including the correlation between the percentage of concentrate and milk production on MUN. The other five correlation values were significantly different (p <0.05). The increase in concentrate intake was moderately correlated (r = 0.52), affecting 27.04% of the increase in milk production, and weakly correlated (r = 0.39), affecting 15.21% of the increase in S/C. The increase in milk production had a very strong correlation (r = 0.82), affecting 67.24% of the increase in S/C. The increase in S/C had a strong correlation (r = 0.61), affecting 37.21% of the increase in CI. The increase in CI was also affected 38.44% by the increase in milk production with a strong correlation (r = 0.62). It could be concluded that dairy cows with higher feed concentrate intake would be followed by an increase in milk production, but had a negative effect on reproduction in the form of increased S/C and CI.

Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra BUDI UTOMO Budi Utomo Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum ERMA SAFITRI Erma Safitri Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas’ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti

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