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All Journal Media Kedokteran Hewan Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Andrology and Biomedical Journal Jurnal Kedokteran Hewan Smart Dedication: Jurnal Pengabdian Masyarakat

Suherni Susilowati

Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya 60115

Author-ID : 3693314

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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Ameliorative Effects of Eleutherine palmifolia Extract on Monosodium Glutamate-Induced Reproductive Toxicity: Modulation of Oxidative Stress Markers and Spermatogenesis in a Murine Model Rahmatullah, Aldin Akbar; Ratnaningtyas, Nurrohmah; Mustofa, Imam; Mulyati, Sri; Sugihartuti, Rahmi; susilowati, Suherni; Putri, Cindy Ercha Aulia
Indonesian Andrology and Biomedical Journal Vol. 6 No. 1 (2025): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v6i1.72293

Background : Chronic monosodium glutamate (MSG) exposure is linked to testicular toxicity, impacting spermatogenesis and oxidative stress markers. Objective : This study investigated the protective effects of Dayak onion (Eleutherine palmifolia) extract against MSG-induced testicular damage in mice. Methods : Twenty-five male mice (11 weeks, ~20 g) were divided into five groups: negative control (C-, 0.5% CMC-Na), positive control (C+, MSG 4 mg/g BW), and treatment groups (T1, T2, T3) receiving MSG plus Dayak onion extract (30, 60, 120 mg/kg BW, respectively) for 52 days. On day 53, testicular histology and serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were analyzed. One-way ANOVA and Duncan’s post hoc test assessed differences (p<0.05). Results : Group C+ showed significantly reduced spermatogenic cell counts, elevated MDA, and decreased SOD compared to C-. Group T1 exhibited no significant improvement in spermatogonia and spermatocytes but increased spermatids. Groups T2 and T3 significantly improved spermatogenic cell counts, with T3 showing the closest recovery to C-. MDA levels decreased, and SOD activity increased dose-dependently, with T3 nearing C- levels. Conclusion : Dayak onion extract, particularly at 120 mg/kg BW, mitigates MSG-induced testicular toxicity, preserving spermatogenesis and balancing oxidative stress markers.

Pemberdayaan Kelompok Asuhan Mandiri Manggala melalui Pemanfataan Tanaman Toga dengan Pupuk Organik Koestanti S, Emy; Suprihati , Endang; Sukartini, Tintin; Hidanah, Sri; Paramita Lokapirnasari, Widya; Susilowati, Suherni; Maslachah, Lilik; Angelina Hendarti, Gracia
Aksiologiya: Jurnal Pengabdian Kepada Masyarakat Vol 8 No 4 (2024): November
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/aks.v8i4.24107

Tanaman Obat Keluarga (TOGA) merupakan jenis tanaman yang memiliki dampak positif secara farmakologis pada tubuh manusia dan umumnya ditanam baik di lingkungan rumah tangga maupun dalam skala komunal. Penelitian ilmiah terhadap tanaman obat keluarga yang memiliki khasiat pengobatan juga mengungkapkan bahwa tanaman tersebut mengandung zat-zat atau senyawa aktif yang terbukti memberikan manfaat bagi kesehatan. Fokus pengabdian masyarakat, meningkatkan pengetahuan dan ketrampilan pada kelompok asuhan mandiri tentang aplikasi pupuk organik sebagai media untuk Taman Toga yang ramah lingkungan dan aplikasi pembuatan jejamuan metode ekstraksi untuk mengeluarkan zat aktif pada tanaman herbal. Hasil evaluasi menunjukkan 100% peserta menanam temulawak, sebanyak 35 peserta (81%) menanam temulawak dan kunir sebanyak 25 peserta menanam temulawak, kunir dan sambiloto (58%). Pengetahuan tentang pupuk organik dari peserta yaitu tidak tahu sebanyak 4 peserta (9,5%), cukup tahu sebanyak 25 peserta (58%) dan sangat tahu sebanyak 14 peserta (32,5%). Sedangkan pemahaman tentang pemanfaatkan pupuk organic terhadap toga didapatkan hasil 29 peserta cukup paham (67,5%), 8 peserta sangat paham (18,5%) dan 5 peserta masih belum paham (12%). Permasalahan tanaman obat pada kelompok asuhan mandiri dapat diatasi dengan cara aplikasi pupuk organik berasal dari kotoran hewan domba yang telah diolah menjadi pupuk yang ramah lingkungan.

Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Sugihartuti, Rahmi; Susilowati, Suherni; Hamid, Iwan Sahrial; Suprayogi, Tri Wahyu; Rachmawati, Kadek; Safitri, Erma; Mustofa, Imam; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.113-122

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (CMC-Na 0.5%), C+ (4 mg/g BW of MSG), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg BW of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The results showed no significant differences in the motility and concentration of spermatozoa between the C- (81.50 ± 4.18; 7.21 ± 0.37) and T3 (77 ± 2.74; 7.04 ± 0.13) groups (p < 0.05). In addition, the results showed significant differences in the viability of spermatozoa between the C- (83.30 ± 3.40) and T3 (77.20 ± 4.06) groups. Therefore, it can be concluded that the administration of Dayak onion extracts orally to mice induced with MSG can maintain the motility, viability, and concentration of spermatozoa.

Protective Effect of The Extract of Dayak Onions (Eleutherine palmifolia) on Sertoli and Leydig Cell Necrosis in Mice (Mus Musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Ratnaningtyas, Nurrohmah; Sugihartuti, Rahmi; Susilowati, Suherni; Safitri, Erma; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.87-96

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the number of necrotic Sertoli and Leydig cells in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (0.5% CMC-Na), C+ (4 mg/g BW of MSG and 0.5% CMC-Na), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The mice were euthanized on day 53 of the experiment. Their testicles were removed and used to prepare histological specimens with the H&E staining method. The results showed significant differences (p < 0.05) in the number of necrotic Sertoli and Leydig cells between the C+ group and the T1, T2, and T3 groups with gradually decreasing values. The results suggested that the administration of the extract of Dayak onions can prevent Sertoli and Leydig cell necrosis in mice induced with MSG at an optimal dose of 120 mg/kg BW.

Pemulihan Fungsi Reproduksi Pasca Penyakit Mulut dan Kuku pada Sapi Perah di Desa Bringin Kecamatan Wajak Kabupaten Malang Susilowati, Suherni; Suprayogi, Tri Wahyu; Lokapirnasari, Widya Paramita; Brahmantya, Chandra; Supriyadi, Supriyadi
Bima Abdi: Jurnal Pengabdian Masyarakat Vol. 5 No. 4 (2025): Bima Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Pendidikan Bima Berilmu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53299/bajpm.v5i4.2584

Penyakit Mulut dan Kuku (PMK) memberikan dampak serius terhadap kesehatan reproduksi dan produktivitas sapi perah di Indonesia. Pasca wabah PMK, banyak peternak mengalami kesulitan dalam memulihkan fungsi reproduksi ternaknya karenam kurangnya pengetahuan mengenai kesehatan reproduksi dan manajemen pakan. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pemahaman peternak tentang manajemen kesehatan reproduksi sapi perah serta mendukung pemulihan pasca PMK. Metode yang digunakan meliputi penyuluhan dengan pre-test dan post-test, serta pemeriksaan kesehatan reproduksi langsung pada sapi perah. Hasil evaluasi menunjukkan peningkatan pemahaman peternak lebih dari 35% pada aspek penyakit PMK, gangguan reproduksi, manajemen pakan, dan kandang. Selain itu, pemeriksaan lapangan menemukan beberapa kasus gangguan reproduksi seperti anestrus, silent estrus, dan repeat breeder yang ditangani melalui terapi hormonal dan perbaikan manajemen pakan. Kesimpulannya, kegiatan ini efektif dalam meningkatkan pengetahuan peternak dan membantu pemulihan fungsi reproduksi sapi perah pasca PMK sehingga mendukung keberlanjutan usaha peternakan.

PENAMBAHAN L – ARGININ DALAM PENGENCER TRIS KUNING TELUR SETELAH EKUILIBRASI 2 JAM TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH SPERMATOZOA DOMBA SAPUDI Suryaningsih, Hastini; Sabdoningrum, Emy Koestanty; Susilowati, Suherni; Sardjito, Trilas; wurlina, wurlina; Damayanti, Tita
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using Analysis of Variance (ANOVA) then proceed to the Duncan Test to determine significant differences between treatments. The first treatment P0 was no L – Arginine added as control. P1 was treated with 0,004 M L – Arginine. P2 was treated with 0,005 M L – Arginine, P3 was treated with 0,006 M L – Arginine. Result of two hour equilibration time for the sperm motility showed: (P0) 46,67a 5,16, (P1) 49,16ab 5,84, (P2) 55,00bc 7,74, (P3) 58,33c 6,83. Result of two hour equilibration time for the sperm plasm membrane were: (P0) 39,33a 4,92, (P1) 43,33ab 4,22, (P2) 46,50 bc 3,44, (P3) 50,83 c 3,48. The addition of L-arginine with a concentration as much as 0.006 M, shows the highest result in sperm motility and sperm plasm membrane intact of Sapudi semen.

PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Nawangwulan, Tantri; Madyawati, Sri Pantja; Plumeriastuti, Hani; Sardjito, Trilas; Susilowati, Suherni; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.

KUALITAS MOTILITAS DAN VIABILITAS SPERMATOZOA DARI SEMEN AFKIR SAPI LIMOUSIN PADA PENGENCER SUSU SKIM KUNING TELUR SITRAT DENGAN PENAMBAHAN BERBAGAI KADAR GLUKOSA Irvanto, Rudi; Hardijanto, Hardijanto; Paramita, Widya; Susilowati, Suherni; Damayanti L, Tita; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Semen observation was done at 0 hour, 24 hours and 48 hours. Research design used in this study was completely randomized design with faktorial pattern with 5 replicates. Highest result in motility this research was showed at 24 hours with 30% value in glucose 2,5% treatment and 48 hours with 10% value. The lowest result showed in glucose 0% treatment at 24 hours and 0% at 48 hours. Highest result in viability showed on glucose 2,5% treatment with 62,6% value at 24 hours and at 48 hours with 53,4% value. Lowest result in viability showed on glucose 0% treatment with 44,2% value at 24 hours and 31,4% value at 48 hours.

PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Rohmah, Lailatul; Triana, Indah Norma; Sunarso, Agus; Susilowati, Suherni; Hidajati, Nove; Kurnijasanti, Rochmah
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon's rind have an ability for antioxidants effect that can reduce sperm's damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon's rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P1: was exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P2: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 20 mg/rat/day, P3: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 40 mg/rat/day, and P4: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat's sperm (Rattus norvegicus) exposed to heat.

EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS Utama, Dirga Januar Surya; Susilowati, Suherni; Hernawati, Tatik; safitri, Erma; Mulyati, Sri; Nurhajati, Tri
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant difference (p> 0.05).

Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra Budi Utomo BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri ERMA SAFITRI Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas’ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti

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