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All Journal Media Kedokteran Hewan Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Andrology and Biomedical Journal Jurnal Kedokteran Hewan Smart Dedication: Jurnal Pengabdian Masyarakat
Suherni Susilowati
Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya 60115
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PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Susilowati, Suherni; Hernawati, Tatik
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.363

Abstract

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untukmeningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukanaplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.
Potential of Basil (Ocimum basilicum) leaf extract in Tris-egg yolk extender on sperm motility and plasma membrane integrity of Sapudi ram at room temperature Pratama Putra Banusiandana; Suherni Susilowati; Sri Mulyati; Tri Wahyu Suprayogi; Emy Koestanti Sabdoningrum; Nove Hidajati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.119-126

Abstract

This study aimed to determine the effect of basil (Ocimum basilicum) leaf extract in a Tris-egg yolk extender on the motility, and plasma membrane integrity of Sapudi ram spermatozoa at room temperature. Fresh semen samples were collected from Sapudi ram using an artificial vagina. A completely randomized design was used, consisting of four treatment groups with five replicates each. Data were analyzed using SPPS with factorial analysis of variance (ANOVA) and Duncan’s test. The control group (T0) received Tris-egg yolk extender without basil leaf extract. Treatment groups T1, T2, and T3 received 1%, 2%, and 3% basil leaf extract, respectively, in the extender. Results showed that sperm motility and plasma membrane integrity (Table 4) tended to decrease with increased storage time. Among the treatment groups, the addition of 1% basil leaf extract in the Tris-egg yolk extender (T1) resulted in significantly higher (p <0.05) both sperm motility and plasma membrane integrity compared to the control (T0). However, higher concentrations of basil leaf extract (T2 and T3) did not result in significant differences (p >0.05) in sperm motility after three hours of storage compared to the 1% treatment (T1). In conclusion, the addition of 1% basil leaf extract to a Tris-egg yolk extender was effective in maintaining sperm motility and plasma membrane integrity of Sapudi ram spermatozoa stored at room temperature for up to three hours.
Supplementation with mineral mix or concentrate booster restored fertility in cows following foot-and-mouth disease infection Aris Puji Nugroho; Ali Agus; Moh. Sofiul Anam; Suherni Susilowati; Herry Agoes Hermadi; Dian Ayu Permatasari; Mohammad Anam Al Arif
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.127-137

Abstract

This study aimed to evaluate the effects of supplementing mineral mix and concentrate booster on the fertility of cows recovering from foot-and-mouth disease (FMD). Eighteen post-FMD cows were assigned to three treatment groups. Cows in the T0 group received a standard feed consisting of elephant grass and a local concentrate. In the T1 group, cows were provided the standard feed supplemented with 40 g/day of mineral mix, while cows in the T2 group received the standard feed supplemented with 600 g/day of concentrate booster. The feeding treatments were applied for 42 days, and fresh drinking water was provided ad libitum. The results showed that the onset of estrus occurred significantly earlier (p <0.05) compared to the anestrus period experienced during FMD infection. Both supplemented groups exhibited higher (p <0.05) estrus rates, pregnancy rates, and calving rates than the T0 group. The interval between the last estrus and the initiation of additional feeding was considerably longer than the onset of estrus observed after treatment. The pH and ferning scores of cervical mucus in cows from the T0 group were lower than those in the T1 and T2 groups. However, no significant differences (p >0.05) were found in cervical mucus pH or ferning scores between the T1 and T2 groups, between pregnant and non-pregnant cows, or between offspring sex categories. In conclusion, supplementation with either a mineral mix or a concentrate booster improved fertility parameter, including estrus rate, pregnancy rate, and calving rate, in cows recovering from FMD.
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
The effect of epigallocatechin 3-gallate (EGCG) on Sertoli and Leydig cells counts and spermatozoa quality of rats (Rattus norvegicus) exposed to monosodium glutamate Indira, Sitti Az-Zahra; A Ashiila Tsabita; susilowati, Suherni; Sri Pantja Madyawati; Yudaniayanti, Ira Sari; kusnoto, kusnoto; Legowo, Djoko; Hidajati, Nove; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.56-64

Abstract

This study aimed to determine the preventive effect of epigallocatechin 3-gallate (EGCG) on the number of Sertoli and Leydig cells in white rats (Rattus norvegicus) exposed to monosodium glutamate (MSG). This study used 25 male rats which were divided into five groups. The C– group was given only 1% Na-CMC, while the C+ group received 1% Na-CMC and 120 mg/kg BW of MSG. The T1, T2, and T3 groups were administered EGCG at doses of 4, 8, and 16 mg/kg BW, respectively, along with 120 mg/kg BW of MSG. All treatments were administered orally for 28 days. After the treatment period, the rats were sacrificed on day 29. Their testes were collected and processed into histological specimens using hematoxylin eosin (H&E) staining. The results showed that MSG exposure significantly decreased (p <0.05) the number of Sertoli and Leydig cells in the C+ group compared to the C– group. In contrast, the T1, T2, and T3 groups showed increased cell counts following EGCG administration. Epigalocathecin-3-gallate at a dose of 8 mg/kg BW showed the best potential in maintaining spermatozoa motility, viability, and plasma membrane integrity in rats exposed to MSG. EGCG, as an antioxidant, can reduce lipid peroxidation in the spermatozoa plasma membrane, thereby improving spermatozoa viability, motility, and plasma membrane integrity. In conclusion, the administration of EGCG mitigates the harmful effects of MSG on Sertoli and Leydig cell counts in white rats, with an optimal dose of 8 mg/kg BW.
Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra Budi Utomo BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri ERMA SAFITRI Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas’ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti