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All Journal HAYATI Journal of Biosciences e-GIGI Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pharmaciana: Jurnal Kefarmasian Zenit Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Global Medical and Health Communication Majalah Obat Tradisional Majalah Kedokteran Science and Technology Indonesia Jurnal Kedokteran Gigi Universitas Padjadjaran Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies JFIOnline Journal Syifa Sciences and Clinical Research (JSSCR) Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Cakradonya Dental Journal International Journal of Electrical, Computer, and Biomedical Engineering (IJECBE) Folia Medica Indonesiana
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Maranatha Christian University Bandung
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Journal : Science and Technology Indonesia

 1 
Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
The Potential of Ethanol Extract of Pasak Bumi Roots (Eurycoma longifolia Jack) as an Anti-Prostate Cancer In Vitro Against PC-3 Cells Kania, Nia; Rahman, Eka Yudha; Priyandoko, Didik; Sabrina, Adilah Hafizha Nur; Widowati, Wahyu; Azis, Rizal; Annaba, Aziz; Hadiprasetyo, Dhanar Septyawan; Alexandro, Garry
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.452-466

Abstract

The prevalence of prostate cancer cases in men is expected to continue increase. In 2040, it is estimated that there will be 2.293.818 new cases and a 1.05% increase in the death rate due to prostate cancer. Eurycoma longifolia Jack roots extract (ELE) has potential as an alternative treatment. This study aims to analyze ELE potential as an anti-prostate cancer agent through in silico assay and in vitro assays on the prostate cancer cell line (PC-3). ELE compounds were docked against Casp-3, Casp-8, HAX-1, p27, and PTEN. In vitro assays on PC-3 cells were used, namely cell viability (WST-8), ROS levels; cell cycle; and cell apoptosis (flow cytometry), PC-3 cell senescence (μ-Galactosidase staining), Casp-3; Casp-8; HAX-1; p27; and PTEN gene expression (qRT-PCR). All proteins target were successfully docked with ELE compounds and presented binding interactions. ELE is known to reduce viability, intracellular ROS levels, live cells, necrosis, and reduce HAX-1 gene expression, and inhibit the cell cycle G0/G1 phase. ELE can also increase inhibition, senescence, late and early apoptosis, and Casp-3, Casp-8, p27, and PTEN gene expression. ELE 100 μg/mL is the most effective concentration. ELE has potential as an anti-prostate cancer agent through apoptosis, cell cycle, and antioxidant pathways
Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Co-Authors , Rizal - Lindayani AA Sudharmawan, AA Afifah Bambang Sutjiatmo, Afifah Bambang Afifah, Ervi Agung Novianto, Agung Agus Susanto Ahmad Faried Alexandro, Garry Annaba, Aziz Arif, Julio Arinta, Yukko Armansyah, Adek Arrahmani, Betharie Cendera Aryenti, Aryenti Ayuni, Vini Azis, Rizal Bangun, Adityana Arta Batubara, Lilian Ch. Retnaningsih, Ch. Chandra Risdian Chiuman, Linda Damayanti, Ndaru Andri Dani Dani Dewi Nurul, Dewi Dewi, Intan Keumala Dewi, Nindia Salsabila Mia Didik Priyandoko Djaja Rusmana Edriayani, Novi Edwin Setiabudi Edy Fachrial Eka Yudha Rahman Elisabeth, Mariska Eltania, The Fransiska Elvira, Cut Endah Purnamasari Esti Mumpuni, Esti Euis Reni Yuslianti, Euis Fahrauk, M. Fanny Rahardja Fauziyah, Nida Firyal Ferdiansyah, Rival Ferdinand, Sahna Ferry Sandra Fibrina, Dewi Fioni, Fioni Geeta, Ginting, Chrismis Novalinda Girsang, Ermi Gosandra, Sherrina O. Gunadi, Michael Sebastian Gunanegara, Rimonta Febby Hadiprasetyo, Dhanar Septyawan Halim, Nathanael HANA RATNAWATI Handayani, Rr. Anisa Siwianti Hanna Sari Widya Kusuma, Hanna Sari Widya Harjanti, Mathelda Weni Harry Murti Hartini Tiono Haryanti, Mathelda Weni Hernawati Hernawati Irfan Syarif, Irfan Irwan, Mulia Jeffrey ., Jeffrey Jo Suherman Johan Lucianus Joko Raharjo, Sentot Juansah, Rahadian Deden Julio Arif Jusri, Ridza Khainir Akbar, Khainir Khie Khiong Ksatrianto, Faris Kusdianti Kusdianti Liana Liana Lindayani, . Lister, I Nyoman Ehrich Lusiana Darsono MADE PHARMAWATI Malau, Reghita Claudia Manurung, Mian Maranata Br Marisca Evalina Gondokesumo, Marisca Evalina Marpaung, Hans Hotma Mars, Nicholas Mawarni, Evi Mulyasari, Tira Endang Muthmainnah, Anis Syabani Nafik, Said Nainggolan, Ita Margaretha Nasution, Ali Napiah Natadilandes, Reyhan Ngurah Intan Wiratmini Nia Kania Nindia Salsabila Mia Dewi Nindya, Faradhina Salfa Ningrum, Siti Ratu Rahayu Novilla, Arina Nunung Ainur Rahmah Nurjamil, Aris Muhammad Nursanti, Okta Obeng, Selonan Philips Onggowidjaja Pradipta, Regina Purwoko, Agus Qamarani, audina Qlintang, Sandy Qodariah, Rismawati Laila Qodriah, Rahmatul R. Sapto Hendri Boedi Soesatyo Rahma Micho Widyanto RAHMAT, DENI Rita Tjokropranoto Rizal Aziz, Rizal Rizal Rizal Sabrina, Adilah Hafizha Nur Sachrowardi, Qomariyah Romadhiyani Salfa Nindya, Faradhina Salsabila, Nindia Santiadi, Sherly Sarwono, Sylvie Saufa, Zahra Qisthi Sholihah, Ika Adhani Sijani Prahastuti Sinaga, Ade Putra Fratama Siregar, Irma Dolsyeria Solihat, Ananda Khairunisa Sormin, Delores Elisabeth Sosiawan, Insan Sri Utami Suci Nar Vikasari, Suci Nar Sugiaman, Vinna Kurniwati Suhantoa, Deviana Lavender Sukandar, Elin Y. Susi Endrini Susy Tjahyani Sutjiatmo, Afifah B Syamsi, Lusi Nursilawati Syamsudin Syamsudin Takasenserang, Oktaviana Teddy Marcus Zakaria Teresa Liliana Wargasetia Tjandrawati Mozef Tri Suciati Triharsiwi, Dwi Nur Udju D. Rusdi Utomo, Herry S Utoyo, Frederick Surya vera vera, vera Vinna K. Sugiaman Vinna Kurniawati Sugiaman, Vinna Kurniawati Vrianty, Dela Wahyu Surakusumah WAHYU WINARNO Wangguai, Vannia Wibowo, Satrio Haryo Benowo Widodo, Wahyu Setia Widya Kusuma, Hanna Sari Widya Kusuma Yuninda, Viranda Andria Yusuf, Lydia Zahira, Nurul Zahra Zahiroh, Fadhilah Haifa