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Identifikasi Bakteri Escherichia coli (E.coli) pada Air Minum di Rumah Makan dan Cafe di Kelurahan Jati serta Jati Baru Kota Padang Wahyu Zikra; Arni Amir; Andani Eka Putra
Jurnal Kesehatan Andalas Vol 7, No 2 (2018)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v7i2.804

Abstract

Air merupakan komponen yang sangat penting dalam kehidupan, seperti penggunanaan air sebagai air minum. Air minum juga digunakan oleh pedagang makanan. Tingginya kasus diare di Kelurahan Jati dan Jati Baru Kota Padang dapat dipicu penggunaan air minum yang tidak bersih. Tujuan penelitian ini adalah menentukan adanya kontaminasi oleh bakteri Escherichia coli dalam air minum pada rumah makan dan cafe di Kelurahan Jati dan Jati baru kota Padang. Penelitian ini bersifat deskriptif dengan perlakuan eksperimental terhadap 16 sampel air minum di Kelurahan Jati dan Jati Baru Kota Padang. Pengambilan sampel secara langsung dengan botol steril. Analisis data dengan menggunakan tabel (Most Probable Number) MPN 5-1-1 dan ditemukannya koloni bakteri Escherichia coli pada sampel air minum. Hasil penelitian ini didapatkan 13 sampel adanya kontaminasi air terhadap bakteri Coliform dengan indeks MPN didapatkan 3 sampel menunjukkan nilai indeks MPN yang tinggi yaitu: 2 sampel dengan indeks MPN 240/100 ml dan 1 sampel dengan indeks MPN 98/100 ml. Berdasarkan pemeriksaan Mac Conkey Agar ditemukan nya 13 sampel terdapat koloni bakteri Escherichia coli.
Kadar Interferon Gamma Kultur Sel Limfosit pada Anak yang Mendapat Vaksinasi BCG Liza Fitria; Andani Eka Putra; Finny Fitry Yani; Darfioes Basir
Sari Pediatri Vol 18, No 1 (2016)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp18.1.2016.21-26

Abstract

Latar belakang. Vaksinasi BCG mempunyai efek proteksi yang bervariasi, menurun dengan bertambahnya umur anak. Perkembangan pengetahuan di bidang biologi molekuler telah mengembangkan pemeriksaan IFN-γ untuk mendeteksi respon vaksinasi.Tujuan. Mengetahui lamanya efek proteksi BCG dan hubungan kadar IFN-γ dengan umur, status gizi, umur mendapatkan vaksinasi BCG dan sarana kesehatan tempat vaksinasi.Metode. Suatu studi cross sectional, stratified random sampling, pada anak yang telah mendapat vaksinasi BCG. Kadar IFN-γ diukur dari sel limfosit yang telah dikultur. Analisis statistik menggunakan uji Anova dan Independent sample t tes.Hasil. Kadar IFN-γ tertinggi pada kelompok umur 4-11 bulan. Terdapat perbedaan bermakna kadar IFN-γ antara kelompok umur 4-11 bulan dengan 1-4 tahun dan 1-4 tahun dengan 5-9 tahun. Tidak terdapat perbedaan bermakna antara status gizi, umur mendapatkan vaksinasi BCG, sarana kesehatan tempat vaksinasi BCG dengan kadar IFN-γ.Kesimpulan. Kadar IFN-γ yang tinggi menunjukkan vaksinasi BCG efektif pada anak, tetapi efektifitasnya menurun seiring dengan pertambahan umur. Sari
Uji Diagnostik Pemeriksaan Antigen Nonstruktural 1 untuk Deteksi Dini Infeksi Virus Dengue pada Anak Megariani Megariani; Rinang Mariko; Amrin Alkamar; Andani Eka Putra
Sari Pediatri Vol 16, No 2 (2014)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp16.2.2014.121-7

Abstract

Latar belakang. Di negara tropis dan subtropis, dengue adalah masalah kesehatan utama. Diagnosis dini sangat penting untuk manajemen infeksi dengue. Nonstruktural 1 merupakan pendekatan baru terhadap diagnosis dengue. Pemeriksaan rapid NS1 dilakukan untuk deteksi dini infeksi virus dengue pada anak.Tujuan. Menentukan nilai diagnostik NS1 mencakup sensitivitas, spesifisitas, nilai duga positif, nilai duga negatif, dan keakuratan NS1 untuk deteksi dini infeksi virus dengue.Metode. Dilakukan penelitian cross sectional terhadap 50 orang anak demam hari ke-1, ke-2 atau ke-3 dengan tes tourniquet positif pada bulan April sampai Desember 2012. Rapid test NS1 dilakukan untuk dibandingkan dengan RT PCR sebagai gold standard.Hasil. Didapatkan 50 orang anak dengan demam dan tes tourniquet positif. Duapuluh lima anak NS1 positif dan 26 RT PCR positif. Rapid test NS1 memiliki sensitivitas 92,3%, spesifisistas 95,8%, nilai duga positif 96 %, nilai duga negatif 92 %, dan keakuratan 94%.Kesimpulan. Rapid test NS1 mempunyai sensitivitas dan spesifisitas yang tinggi untuk deteksi dini infeksi virus dengue pada anak.
Faktor yang Berhubungan dengan Terjadinya Infeksi Clostridium Difficile pada Penderita Diare akibat Antibiotik Delfican Delfican; Yusri Dianne Jurnalis; Hafni Bachtiar; Andani Eka Putra
Sari Pediatri Vol 15, No 6 (2014)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp15.6.2014.408-14

Abstract

Latar belakang. Pemakaian antibiotik yang tidak rasional dapat menyebabkan Clostridium Difficile Associated Diarrhea (CDAD).Tujuan. Mengetahui proporsi CDAD, faktor yang berhubungan, dan antibiotik yang paling banyak menimbulkan CDAD.Metode. Penelitian potong lintang terhadap anak yang mendapat antibiotik pada bulan Maret-September 2013. Clostridium Difficile Associated Diarrhea adalah ditemukannya gen tcdB C. difficile dari pemeriksaan PCR. Faktor risiko adalah umur, status gizi, jumlah, dosis, cara dan lama mendapat antibiotik. Analisis dilakukan secara bivariat dan multivariat.Hasil. Terdapat 82 orang anak dengan proporsi CDAD 39%. Persentasenya lebih tinggi pada usia 1 bulan-5 tahun, mendapat lebih dari 1 jenis antibiotik, dosis biasa, pemberian oral dan mendapat antibiotik kurang dari 7 hari dengan nilai p >0,05. Persentase CDAD hampir sama pada anak gizi baik dan gizi kurang. Persentase CDAD tertinggi didapatkan pada anak yang mendapat amoksisilin oral.Kesimpulan. Proporsi CDAD cukup tinggi. Usia muda, jumlah antibiotik, pemberian dosis biasa secara oral dan waktu pemberian yang singkat menunjukkan kecenderungan menderita CDAD lebih besar, walaupun secara statistik tidak bermakna.Usia merupakan faktor paling dominan. Persentase CDAD tertinggi pada anak yang mendapat amoksisilin oral.
KLONING DAN OVEREKSPRESI PROTEIN P24-GAG HIV (Cloning and Overexpression P24-Gag of HIV) Efrida Efrida; Andani Eka Putra
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i1.1218

Abstract

HIV diagnosis is confirmed by viral culture, but this process takes a long time. Another method used to detect HIV-specific antigensor antibodies is by immunoassay. Generally, antigen-antibody based methods are used as a screening test. Based on the stability ofthe sequences found in the first (1) study year, the researchers designed this study for the production of p24 recombinant protein.These proteins will be developed as diagnostic markers based on sero-immunology technique. The aim of this study was to know theconstruction and over expression of protein p24gag from local isolates and analysis of the diagnostic potential of doing design specificprimers against p24gag protein, cloning and over expression of the gene, as well as to obtain a p24 protein that has been purified.This research results will be applied later to develop a method based on local isolates of HIV diagnosis. This research was a descriptivestudy, conducted over seven (7) months in the Biomedical Laboratory of the Faculty of Medicine, Andalas University and Departmentof Clinical Pathology, Dr. M. Djamil Hospital, Padang. This study was carried out by using samples of local isolates originating fromthe first year of research. Stages of the research were: 1) the design of primers for cloning, amplification and sequencing, 2) cloninginto pDEST and pENT, 3) transformation of the target gene, 4) detection of fragment insertions, 5) protein expression and proteinanalysis by SDS-PAGE, immunoblotting and 6) purification. The conclusions of this study were: the design of specific primers againstp24gag protein used fragments attb1, attb2, Shine Delgano and Kozac effective for protein expression. The results showed that thepresence of protein 24kDa expression was identical to HIV p24gag protein. Further research needs to be conducted to identify potentialimmunological target protein.
SEKUENS TERAWETKAN TERKAIT HIV-1 Efrida Efrida; Andani Eka Putra
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1081

Abstract

Human Immunodeficiency Virus-1 (HIV-1) have the high levels of genetic variability (molecular variation) related to the mechanismof deletions, insertions, and especially as a recombination. The recombination between subgroups and their inter subtype HIV-1 producesa new virus strain causing a certain infection. Until now there were about 40 circulating recombinant form (CRF) and 100 uniquerecombinant forms (URF) worldwide. Various HIV-1 molecular variation cause pitfalls HIV detection due to the difficulty test designed todetect all HIV-1 strains. The purpose of this study was to know the determination of the molecular variation that can identify the HIV-1genome sequences which are conserved from local isolates of West Sumatera. The method used is a descriptive study on samples of patientssuspected of being infected by HIV-1 based on the antibody screening results using rapid HIV-1 test in the laboratory of Clinical Pathologyat Dr. M. Djamil Hospital Padang. The RNA isolation and amplification by RT-PCR was performed at the Microbiology Department,Faculty of Medicine of UGM. The amplicons were sequenced using the Sanger method and analyzed by Clustal W with sequence datafrom the HIV its database. The major subtypes of HIV-1 isolates derived from local West Sumatra is AE/B with four (4) isolates (57.1%),followed by subtype AE, 2 isolates (28.6%) and B, 1 isolates (14.3%). Most of the molecular variation in this study related to thesubstitution of one base followed by deletions and insertions. The protease showed a wide variation, which consists of 20–37 substitutionsand 1–3 deletions. p24 is a highly conserved gene, followed by gp120 and vpu. Based on this study, it can be concluded that the isolatesof HIV-1 recombination is the biggest proportion with conserved region found at p24 of protein group of the gag.
Penyuluhan dan Pemeriksaan Leptospirosis terhadap Petani di Nagari Alahan Panjang Kabupaten Solok Elizabeth Bahar; Netti Suharti; Roslaili Rasyid; Andani Eka Putra; Linosefa Linosefa; Muhammad Reza; Arni Amir
Warta Pengabdian Andalas Vol 26 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.26.2.88-96.2019

Abstract

Leptospirosis is an infectious disease caused by Leptospira interogans (L interogans). The urine of rats is a carrier of L interrogans bacteria that pollutes stagnant water such as flooding both in rice fields and fields. Bacteria enter through the mucosa and sores or blisters when in contact with the polluted water. The mortality rate due to leptospirosis in Indonesia is reported to reach 2.5 - 16.45%, because it is a tropical country with quite high rainfall followed by flooding. Nagari Alahan Panjang Solok Regency is located at an altitude of 1400 - 1600 meters above sea level (masl) with rainfall of 2600 mm which is a vast agricultural area and the community is 90% farmers. Based on the problem above, the Department of Microbiology and Biology, Faculty of Medicine, Andalas University has held community service in Nagari Alahan Panjang, Solok Regency, for farmers, because farmers are at risk of being infected by Leptospira. The counseling method uses a power point slide and poster about leptospira followed by blood tests using the Standard Q rapid test for Leptospira IGM / IgG. About 48 farmers carried alcohol swabs at the tips of their fingers to take blood samples with fingersticks. As much as 10 ul of blood add 1 drop of standard buffer solution Q rapid test Leptospira IgM / IgG put in the rapid test tool well while stirring and wait 5 minutes. Positive results appear in the red line in the IgM / IgG area according to the control indicated on the rapid test tool. The conclusion that counseling can increase the knowledge of farmers to prevent leptospirosis with the discovery of 6.25% of farmers suspected of leptospirosis.
Peningkatan Komitmen Penanggulangan Covid-19 Mahasiswa melalui Webinar dan Deklarasi SIAGA SAKATO Liganda Endo Mahata; Fitrisia Amelin; Fathiyyatul Khaira; Denada Florencia Leona; Andani Eka Putra; Rizanda Machmud; Irvan Medison; Hardisman Hardisman; Yefri Zulfiqar; Fenty Anggraini; Linosefa Linosefa; Desy Nofita Sari; Rahmat Syawqi
Warta Pengabdian Andalas Vol 28 No 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.28.3.225-231.2021

Abstract

The Covid-19 virus is transmitted from human to human through close contact with infected people through their respiratory droplets. A person can also be infected by touching an object contaminated with SARS-CoV-2 and then followed by reaching the eyes, nose, or mouth. Implementing the health protocol is necessary to reduce the number of virus transmissions. In addition, to increase the community's immune against Covid-19, WHO and the government have been developing a vaccination program. However, the recommendation from the latest research stated that people still have to obey the health protocol even after being vaccinated. The purpose of this community service was to help the government to increase people's understanding of the importance of implying health protocols, even after vaccination, to suppress Covid-19 cases. The method used is by holding an online event called a Webinar. It followed by a statement with audiences to commit to implementing the health protocol led by the Rector of Andalas University, then called a SIAGA SAKATO declaration. The activity began with a pre-test questionnaire for initial information collection, followed by education about Covid-19 prevention from the experts to the 512 students. The activity ended with the final data collection using a posttest questionnaire to test the student knowledge after the education. Paired t-test analysis showed a significant increase in student commitment after attending the Webinar (P≤0.05). Based on these results, the Webinar increases students' understanding of the importance of the health protocol in reducing the Covid-19 cases.
Hubungan Usia, Jenis Kelamin Dan Gejala Dengan Kejadian COVID-19 di Sumatera Barat Nia Ayuni Putri; Andani Eka Putra; Rinang Mariko
Majalah Kedokteran Andalas Vol 44, No 2 (2021): Online July 2021
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v44.i2.p104-111.2021

Abstract

Latar Belakang : Pandemi COVID-19 menjadi masalah global. Di Indonesia angka prevalensi COVID-19 terus meningkat. COVID-19 merupakan penyakit yang disebabkan oleh virus SARS-CoV-2. SARS-CoV-2 menggunakan ACE2 sebagai reseptornya untuk masuk ke dalam sel. Maka dari itu, penelitian ini bertujuan untuk melihat hubungan karakteristik berupa usia, jenis kelamin dan gejala dengan kejadian COVID-19 di Sumatera Barat Metode : Case Control Study dengan sampel berupa spesimen swab nasofaring Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi (LPDRPI) yang diisolasi dan diperoleh isolat RNA virus. Isolat yang diperoleh kemudian diamplifikasi dengan metode qRT-PCR. Hasil : Tidak ada hubungan antara karakteristik jenis kelamin dengan kejadian COVID-19 dimana nilai P value yaitu 0,485 dan lebih besar dari 0,05 (Ho ditolak). Sedangkan terdapat hubungan antara karakteristik usia dan gejala terhadap kejadian COVID-19 dimana nilai P value yaitu 0,000 dan 0,036 yang lebih kecil dari 0,05 (Ho diterima). Kesimpulan : Laki-laki maupun perempuan memiliki probabilitas yang sama untuk terinfeksi COVID-19. Kelompok usia <50 tahun lebih berisiko terinfeksi COVID-19 daripada kelompok usia ≥50 tahun. Lebih banyak orang tanpa gejala daripada orang dengan gejala yang terinfeksi COVID-19. 
PENGARUH PEMBERIAN VAKSIN BCG SECARA ORAL DAN SUBKUTAN TERHADAP KOMPONEN SELULER DAN HUMORAL PADA Rattus norvegicus GALUR WISTAR Netti Suharti; Andani Eka Putra
Majalah Kedokteran Andalas Vol 35, No 2 (2011): Published in August 2011
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.203 KB) | DOI: 10.22338/mka.v35.i2.p117-125.2011

Abstract

AbstrakVaksinasi BCG merupakan metoda pencegahan infeksi M. tuberculosis dengan rentang efektivitas antara 0 – 80%. Vaksin ini bekerja dengan meng-induksi sistem imunitas seluler, terutama sel limfosit T. Beberapa faktor yang mempengaruhi efektivitas vaksin, seperti bahan dasar, rute pemberian, penyimpanan dan lain sebagainya.penelitian ini bertujuan untuk menilai pengaruh rute vaksinasi BCG, per oral dan subkutan terhadap imunitas seluler dan humoral, yang meliputi pembentukan antibodi, kosentrasi sel limfosit B, T dan penilaian kemampuan makrofag.Penelitian eksperimental yang dilaksanakan dengan mengunakan desain post test only control group design. Jumlah sampel 10 ekor Rattus norvegicus galur Wistar. Antibodi diperiksa dengan menggunakan ELISA, serta dilakukan uji statistik mengunakan t-test.Didapatkan kadar antibodi kelompok subkutan pada hari ke-10 adalah OD 1.714 ± 0.124, sedangkan kelompok oral adalah 1.256 ± 0.243 dan pada kontrol kadar antibodi adalah 0.975 ± 0.253 (p < 0.05). Jumlah limfosit B pada kelompok subkutan adalah 4.5 x 103 sel/ml, kelompok oral 2.6 x 103 sel/ml dan kontrol 2.2. x 103 sel/ml (p < 0.05). Jumlah limfosit T kelompok subkutan adalah 3.8 x 104 sel/ml sedangkan pada kelompok oral dan kontrol, masing-masing adalah 1.1 x 104 sel/ml dan 0.8 x 104 sel/ml (p < 0.05). Kemampuan fagositosis makrofag subkutan adalah 62.5 ± 6.8 lebih tinggi dibanding kelompok oral 48.7 ± 5.8 dan kontrol 35.6 ± 4.4 (p < 0.05). Fenomena yang sama ditemukan pada hari ke-20. Namun demikian tidak ditemukan perbedaan konsentrasi seluruh variabel seluler dan humoral antara hari ke-10 dan 20 (p > 0.05).Vaksinasi BCG akan meningkatkan respon imunitas seluler dan humoral dan rute vaksinasi subkutan lebih baik dibanding per oral ditinjau dari aspek respon imun.Kata kunci : Vaksinasi BCG, seluler, humoral, makrofagAbstractBCG vaccine is a method to prevent the infection of M tuberculosis with effectiveness range between 0 – 80%. This vaccine works with inducing cellular immunity system, mainly in cell of Lymphocyte T. There are many factors thatARTIKEL PENELITIAN118influence the vaccines affectivity, such as basic substance, giving route,and storage.This experiment is conducted in to Rattus norvegicus Wistar Furrow in order to examine the effect of the route of BCG vaccination’s in oral and sub cutaneous to cellular and humoral immunity, which covers the formation of antibody, lymphocyte B cell concentration, and the evaluation of macrophage capability.This experiment’s result shows that the antibody of sub cutaneous groups on day 10th is OD 1.714 ± 0.124, and 1.256 ± 0.243 in oral, while in antibody control is 0.975 ± 0.253 (p < 0.05 ). The amount of Lymphocyte B in sub cutaneous group is 4.5 x 103 cell/ml, 2.6 x 103 cell/ml in oral and 2.2 x 103 cell/ml (p < 0.05) in control. The amount of Lymphocyte T in sub cutaneous is 3.8 x 104 cell/ml, while in oral and control is 1.1 x 104 cell/ml and 0.8 x 104 cell/ml (p < 0.05). The phagocytosis ability of sub cutaneous’s macrophage is 62.5 ± 6.8 which is higher than oral group 48.7 ± 5.8 and control 35.6 ± 4.4 (p < 0.05). The same phenomenon also found in day 20th. However, the difference concentrations of the entire cellular and humeral variable between day 10th and day 20th ( p < 0.05 ) has not been found.Based on the result of this experiment’s it can be concluded that BCG vaccination will increase the cellular and humeral immunity response and sub cutaneous’s vaccination route is better that oral, from immunes response aspect side.Key word : BCG Vaccination, cellular, humeral, macrophage.
Co-Authors - Johan Abdiana Abdiana, Abdiana Adang Bachtiar Ade Asyari Afida Razuna Ave Afrizal Agustian, Dede Rahman Aisyah Elliyanti Akmal Djamaan Alioes , Yustini Amelin, Fitrisia Amirah Zatil Izzah Amrin Alkamar Anavelda, Aura Putri Annisa Widi Rizkia Antonius, Puja Agung Arfai, Arfai Arfiandi Arfiandi Ariani, Tutty Arni Amir Arni Amir arniamir, Arni Amir Asri, Ennesta Bari'ah, Puti Asma Basyir, Vaulinne Besri, Hanifa Zahra Bobby Indra Utama Cimi Ilmiawati, Cimi Darfioes Basir Deddy Saputra Dede Agustian Rahman Defni, - Defrin, Defrin Delfican Delfican Delfilaura, Asyifa Delmi Sulastri Densi Selpia Sopianti Densi Selpia Sopianti Desmawati Desmawati Dessy Arisanty Desy Nofita Sari Dewi Gulyla Hari Djanas, Dovy Edison, Ebill Fuji Efrida Efrida Eka Fithra Elfi, Eka Fithra Elizabeth Bahar Elizabeth Bahar Elmatris Sy Eryati Darwin Eti Yerizel Fahma, Siskalil Farashanda, Meysha Farras, Umar Al Fathiyyatul Khaira Fauzul Azhim Febrianti, Ika Kurnia Feby Resicha Fenty Anggraini Finny Fitry Yani Fiona Dewanti Firdawati, Firdawati Fitri Anggraini, Fitri Fitri Nova, Fitri Fitri, Azdiana Fitrina, Dewi Wahyu Gardenia Akhyar Gestina Aliska Hafni Bachtiar Hanif, Monica Mulnia Hanif, Rayhendra Hardisman Hasmiwati Haviz Yuad Haviz Yuad Helmizar Hidayanti, Rahmi Husna Yetti Husna, Annisa Tamara Husni Husni Ida Parwati Ika Kurnia Febrianti Ilmiawati, Ilmiawati Irrahmah, Miftah Irvan Medison Jamsari Jamsari Kaharudin Kaharudin Kaisar , Kevin Florentino Kosno Suprianto Leona, Denada Florencia Linosefa Linosefa Liza Fitria M. Alfian Agustian Mahata, Liganda Endo Malinda Meinapuri Marlina . Megariani Megariani Menkher Manjas Mentari Faisal Putri Mila Permata Sari Muhammad Rayhan Firdaus Muhammad Reza Nelmi Silvia Netti Suharti Netti Suharti, Netti Nia Ayuni Putri Nila Kasuma Novita Ariani Nur Indrawaty Lipoeto Nur, Yuliati Shafan Nurhayati, Nurhayati Oky Masir Prima Indra Putri Rizki Fitriani Putri, Mentari Faisal Rahmadian, Rizki Rahmat Syawqi Rahmi Lestari Rahmi, Wahida Ramadhan, Mario Arya Ranne Balqis RAVEINAL RAVEINAL Rheta Elkhaira Rinang Mariko Rinang Mariko Rita Hamdani Rizanda Machmud Rosfita Rasyid Roslaili Rasyid Roza Sri Yanti Russilawati, Russilawati Rustini Rustini Salmiah Agus Salmiah Agus Saptino Miro Sef Zani Meria silviayolanda, Silvia Yolanda Sinai, Nadiva Kezia Sosmiarti, Sosmiarti Sriyanti, Roza Suharti , Netti Suzana Devi Syamel Muhammad Tarigan, Herri Novita Tresnaningtyas, Sekar Asri Tri Anggraini, Fika Tyas, Syanindhita Wikanthining Utama, Bobby Indra Utami, Refi Amalia Wahyu Zikra Wahyuni, Yosha Putri Wenny Nursa Octarina Wiwik Andriani Yefri Zulfiqar Yessi Widya Putri Yuad, Haviz Yufri Aldi Yufri Aldy Yusri Dianne Jurnalis