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Perbandingan Metode Boiling Dan Kit Komersial Untuk Ekstraksi DNA TB, Tinjauan Dari Aspek Kualitas, Kuantitas, Dan Integritas DNA. Taufiqulhakim, Muhammad Rafi; Linosefa; Syahrul, Muhammad Zulfadli; Putra, Andani Eka
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 4 (2025): Desember 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i4.1399

Abstract

Latar Belakang: Tuberculosis (TB) merupakan suatu penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis. Prevalensi penyakit tuberkulosis pada tahun 2023 meningkat dari tahun 2022, hal ini juga disebabkan karena lamanya diagnosis dari penyakit tuberkulosis. Polymerase Chain Reaction (PCR) merupakan salah satu teknologi molekular yang berkembang untuk mendiagnosis penyakit tuberkulosis. Objektif: Penelitian ini bertujuan untuk menganalisis perbedaan ekstraksi DNA TB dengan menggunakan metode boiling dan kit komersial, yang ditinjau dari aspek kualitas, kunatitas, dan integritas DNA. Metode: Penelitian ini merupakan penelitian analitik. Penelitian ini dilakukan di Pusat Diagnostik dan Riset Penyakit Infeksi Fakultas Kedokteran Universitas Andalas Padang pada periode bulan Juli 2023 - Maret 2024. Hasil: Pada aspek kualitas DNA, ekstraksi DNA dengan menggunakan metode boiling memiliki rata-rata sekitar 1.47 dan kit komersial memiliki rata-rata sekitar 1.79. Pada aspek kuantitas DNA, ekstraksi DNA dengan menggunakan metode boiling memiliki rata-rata 7.1 ng/µl dan kit komersial memiliki rata-rata sekitar 17.1 ng/µl. Pada aspek integritas DNA metode boiling dapat memvisualisasikan 28 sampel positif  dan kit komersial 22 sampel positif. Kesimpulan: pada penelitian ini terdapat perbedaan yang signifikan antara metode boiling dan kit komersial pada aspek kualitas dan kuantitas DNA, dan tidak terdapat perbedaan yang signifikan antara metode boiling dan kit komersial pada aspek integritas DNA. Kata kunci:  Mycobacterium tuberculosis, Ekstraksi, DNA, Boiling, PCR  
CORRELATION BETWEEN FOOD QUALITY AND MICROBIOTA PROFILE OF PREGNANT WOMEN IN THE CITY OF PADANG : FOOD QUALITY AND MICROBIOTA PROFILE Sary, Rizka Yulia; Helmizar, Helmizar; Putra, Andani Eka
INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD) Vol. 7 No. 2 (2025): INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT
Publisher : STIKes Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36566/ijhsrd/Vol7.Iss2/316

Abstract

Background: Pregnancy is a complex physiological period that requires systemic adaptation of the body. During pregnancy, a balanced blood microbiota plays an important role in maintaining the mother's immune system and supporting fetal development. This study aims to determine the correlation between food quality and the microbiota profile of pregnant women in Padang City. Methods: This is an observational study with a cross-sectional design. The study was conducted from January to March 2025 at the Centre for Diagnostic and Research on Infectious Diseases (PDRPI) Laboratory, Andalas University. The sample size was 20 participants, selected using total sampling. Microbiota profiling was performed using 16S rRNA metagenomic sequencing. The results of the microbiota profile analysis were analyzed using Microbiome Analysist software. Results: Statistical analysis showed a p-value of 0.14702 and a t-statistic of -1.5182, indicating that there was no statistically significant difference between food quality and microbiota profile. The comparison of the Firmicutes/Bacteroidetes ratio (F/B ratio) was 1.06, indicating a balanced microbiota in pregnant women. Conclusion: Although not statistically significant, these differences in microbiota composition are biologically important and suggest a potential association between dietary patterns and systemic health in pregnant women.
PENGEMBANGAN PETERNAKAN AYAM BROILER SEBAGAI ALTERNATIF PENINGKATAN EKONOMI DAN KETAHANAN PANGAN MASYARAKAT DI NAGARI SAKO PASIA TALANG KABUPATEN SOLOK SELATAN Sosmiarti Sosmiarti; Yuliati Shafan Nur; Arfai Arfai; Andani Eka Putra
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 8 No. 2 (2025)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v8i2.754

Abstract

In Nagari Sako Pasia Talang, Sungai Pagu District, South Solok Regency, the scarcity of accessible animal protein sources coupled with limited employment opportunities poses a considerable socio-economic challenge. This programme was designed to enhance household incomes and fortify food security through the structured development of broiler poultry farming. The implementation strategy encompassed community outreach via structured training sessions and hands-on workshops, alongside expert consultations facilitated by academics from Andalas University. An integrated educational and participatory approach was employed, incorporating technical training in broiler husbandry, the construction of standardized poultry housing, feed formulation utilizing locally available ingredients, livestock health management, and farm business financial analysis. Tangible support was also provided in the form of poultry housing units, day-old chicks (DOC), feed supplies, and essential farming equipment. The intervention yielded marked improvements in both the knowledge base and practical skills of participating community members, enabling the adoption of efficient, locally adaptive broiler farming techniques. A pilot, collectively managed poultry enterprise was successfully established, producing 970 market-ready broilers from an initial stock of 1,020 DOC, while achieving a competitive feed conversion ratio (FCR). Financial analysis revealed a potential net profit of IDR 9,131,160 per 35-day production cycle, underscoring the venture’s economic viability. Beyond income generation, the programme broadened community access to affordable and nutritious animal protein, and catalyzed the emergence of new, livestock-based entrepreneurial models. With its demonstrable success, the initiative holds significant potential for replication as a sustainable, village-centered strategy for economic resilience and food security. 
Prevalensi Infeksi Cytomegalovirus (CMV) Berdasarkan Jenis Sampel dan Karakteristik Pasien Balita di Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi Universitas Andalas Tahun 2023–2024 Sekar Asri Tresnaningtyas; Andani Eka Putra; Meysha Farashanda; Siskalil Fahma; Monica Mulnia Hanif
Medika: Jurnal Ilmiah Kesehatan Vol. 5 No. 2 (2025): Medika: Jurnal Ilmiah Kesehatan
Publisher : Universitas Nahdlatul Ulama Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69503/medika.v5i2.1126

Abstract

Infeksi Cytomegalovirus (CMV) merupakan salah satu infeksi virus yang berpotensi serius pada anak balita, terutama karena potensi komplikasi jangka panjang. Penelitian ini bertujuan untuk mengetahui prevalensi CMV berdasarkan jenis sampel (urin dan darah) serta karakteristik pasien balita yang diperiksa di Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi Fakultas Kedokteran Universitas Andalas selama tahun 2023 hingga 2024. Penelitian dilakukan secara deskriptif analitik terhadap 87 data pasien anak usia 0–28 bulan. Analisis statistik meliputi uji Kruskal-Wallis, chi-square, dan McNemar. Hasil menunjukkan bahwa sebagian besar pasien memiliki hasil positif CMV pada kedua spesimen, yaitu urin dan darah (42,5%), diikuti oleh positif hanya pada urin (18,4%), hanya pada darah (14,9%), dan negatif pada keduanya (24,1%). Tidak terdapat hubungan signifikan antara jenis kelamin atau usia dengan hasil CMV. Uji McNemar menunjukkan tidak ada perbedaan bermakna antara hasil pemeriksaan urin dan darah (p = 0,710). Dapat disimpulkan bahwa baik urin maupun darah memberikan hasil yang sebanding, meskipun dengan konsistensi terbatas.
P PENGUATAN PERAN BUMNAG SEBAGAI UNTUK MENINGKATKAN PENDAPATAN NAGARI DAN MASYARAKAT SAKO PASIA TALANG KECAMATAN SUNGAI PAGU KABUPATEN SOLOK SELATAN: Participatory Institutional Strengthening of Village-Owned Enterprises to Promote Community Economic Development Sosmiarti Sosmiarti; Fadhil Aptana Sadjiana; Ferry Lismanto Syaiful; Yuliati Shafan Nur; Andani Eka Putra
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 9 No. 2 (2026)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v9i2.756

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Village-Owned Enterprises (VOEs) play a strategic role in promoting local economic development and improving community welfare in rural Indonesia. However, many VOEs, including VOEs Sako Jaya in Sako Pasia Talang Village, South Solok Regency, face persistent challenges, including weak institutional governance, limited human resource capacity, inadequate access to capital, and weak stakeholder collaboration. This study aimed to identify the institutional potential and challenges of VOEs Sako Jaya and strengthen its institutional capacity through a participatory, multi-stakeholder approach. A community-based participatory approach was implemented through preliminary surveys, field observations, focus group discussions, participatory workshops, mentoring, and monitoring and evaluation. Data were analyzed using descriptive qualitative methods based on observations, documentation, and group discussions. The study involved VOEs managers, village government officials, MSME actors, academics, local government representatives, and private-sector partners. A total of 56 participants from nine villages, nine VOEs, and twelve MSMEs were involved. The findings identified three major institutional challenges: limited human resource capacity, inadequate business capital, and weak organizational governance and coordination. An institutional strengthening plan was subsequently developed, broiler chicken farming was selected as the priority business based on local potential and market demand, and a tripartite partnership involving the South Solok Regency Government, Universitas Andalas, and Supreme Energy was established to support financing, capacity building, and technical assistance. Overall, the participatory approach effectively strengthened institutional capacity, expanded strategic partnerships, and enhanced organizational readiness for sustainable rural enterprise development, providing a replicable model for strengthening village-owned enterprises and promoting community economic development.
Hubungan Infeksi Gardnerella vaginalis Dengan Manifestasi Klinis Keputian Penyebab Vaginosis Bakterialis Pada Ibu Hamil Ketuban Pecah Dini Di RSUP Dr. M. Djamil Padang Fitri Anggraini; Andani Eka Putra; Vaulinne Basyir
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.11911

Abstract

Premature rupture of membrane (PROM) is one of the abnormalities found in pregnancy. The results find out that Lactobacillus is associated with the reduced risk of premature rupture of membrane (PROM), while Gardnerella, Prevotella, Megasphaera, Ureaplasma and Dialister are associated with the increase risk of PROM. Gardnerella vaginalis, a facultative anaerobic bacterium, is the most common microbe associated with bacterial vaginosis (BV).The research used cross sectional research design with retrospective data collection method. The research included all vaginal swab samples from pregnant women with premature rupture of membranes at RSUP Dr. M. Djamil, Padang. The sampling technique used was consecutive sampling, with 31 samples from pregnant women who experienced premature rupture of membranes, complemented with data from electronic/manual medical records. Clinical data collected included complaints such as vaginal discharge. The microbes that cause bacterial vaginosis were examined using Real-Time PCR at the PDRPI Laboratory, Faculty of Medicine, Universitas Andalas. Data then were analyzed using the chi-square test, followed by calculation of the Odds Ratio (OR).he results showed no significant association between the presence of G. Vaginalis and vaginal discharge (p = 0.384; OR = 2.67), Based on the analysis, most bacterial vaginosis infections occurred without the evident of clinical manifestations (asymptomatic) which caused to delayed diagnosis and an increased likelihood of cases being inadequately managed.The conclusion of this research shows that there is no significant association between microbes of bacterial vaginosis and manifestation of vaginal discharge in pregnant women. Antenatal care (ANC) interventions should include the development of a routine vaginal infection screening system within ANC services. Keywords: Gardnerella vaginalis, Bacterial Vaginosis, Pregnant Women
Identification of Gender in Early Pregnancy Using Cell-Free Fetal Dna Budi Junio Hermawan; Bobby Indra Utama; Andani Eka Putra
Andalas Obstetrics And Gynecology Journal Vol. 10 No. 1 (2026)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.10.1.135-144.2026

Abstract

Introduction: Traditionally, chorionic villus sampling and amniocentesis can be used to help identify fetal gender around 11 weeks of pregnancy. However, the risk of miscarriage with these procedures is approximately 1 to 3%. The discovery of cell-free fetal DNA (cffDNA) fragments in the plasma and serum of pregnant women carrying male fetuses has made reliable and accurate diagnosis using non-invasive procedures an option for determining fetal gender. To determine the success rate of fetal gender identification in early pregnancy using Cell Free Fetal DNA. Methods: An analytical observational research method to identify fetal gender in early pregnancy through Cell Free Fetal DNA examination in maternal blood. A total of 70 samples were collected using quota sampling technique. Data analysis was performed using sensitivity and specificity tests, as well as cross-sectional analysis to determine the relationship between PCR test results and gender at birth.      Results: The PCR test has low sensitivity (14.29%), meaning the test is less effective in correctly detecting male gender, resulting in many false negatives. However, it has high specificity (98.11%), indicating its excellent ability to detect female gender with very few false positives. Additionally, the Pearson Chi-Square statistical test resulted in a p-value of 0.033 (p < 0.05), indicating a statistically significant relationship between PCR test results and gender at birth.   Conclusion: Statistically, this test is better at identifying females than males, with a lower error rate for females.  
Analysis of bile acid in saliva of patients with laryngopharyngeal reflux and non-laryngopharyngeal reflux Ade Asyari; Refi Amalia Utami; Eti Yerizel; Andani Eka Putra; Firdawati Firdawati
Oto Rhino Laryngologica Indonesiana Vol. 53 No. 2 (2023): VOLUME 53, NO. 2 JULY - DECEMBER 2023
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v53i2.561

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ABSTRACT — Background: Laryngopharyngeal reflux (LPR) is the backflow of gastric and or duodenal fluid into the larynx, pharynx, trachea, and bronchi. The prevalence of LPR is difficult to determine due to the limited gold standard and the large variety of LPR symptoms. Damage can occur due to the decrease in pH value and also because of exposure to harmful enzymes in reflux, including bile acid. Purpose : This study was conducted to analyze bile acid levels in the saliva of LPR patient and non LPR subject. Methods:  This study is an observational study with a case-control design. The study was conducted in the ORL-HNS Department of Dr. M. Djamil Hospital, Padang, West Sumatra, Indonesia. The total sample size was 44 people. We enrolled 22 healthy subjects as the control group and 22 patients suspected of having LPR. Result: LPR patients are more common in women than in men, with 12 women and 10 men. Bile acid in the LPR group means of is 25.08±7.67µM, meanwhile, in the healthy group, the mean was 18.99±8.26 µM. There is a statistically significant in the incidence of LPR with the bile acids (p = 0.015) based on t- independent test. Conclusion: Our study confirmed that bile acids in saliva play a major role in diagnosing LPR. 
Codon Optimized MMLV Reverse Transcriptase Expression and Functional Evaluation in Escherichia coli Elsa Alfiyanti; Andani Eka Putra; Rita Maliza; Alponsin Alponsin
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12798

Abstract

Dependence on imported reverse transcriptase (RT) enzymes remains a challenge for the development of molecular diagnostic reagents, particularly in developing countries. This study aimed to design, express, purify, and functionally evaluate a recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) as a candidate locally produced molecular biology reagent. A codon-optimized synthetic MMLV-RT gene was cloned into the pET-21a(+) expression vector and transformed into Escherichia coli BL21 (DE3). Recombinant protein expression was induced using IPTG, followed by purification using Ni²⁺-NTA affinity chromatography and characterization using SDS-PAGE. Functional activity of the recombinant enzyme was evaluated through reverse transcription of RNA templates followed by conventional PCR amplification. The optimized MMLV-RT gene was successfully expressed in E. coli BL21 (DE3), producing a recombinant protein band at approximately 55.4 kDa. The highest protein yield was obtained after overnight induction, reaching 3.385 mg/mL. Purification resulted in a dominant protein band corresponding to the expected molecular weight of MMLV-RT. Functional evaluation demonstrated that recombinant MMLV-RT successfully generated specific PCR amplicons with performance comparable to commercial reverse transcriptase without detectable non-specific amplification. These findings indicate that recombinant MMLV-RT produced in E. coli BL21 (DE3) retains functional reverse transcriptase activity and has potential for further development as an alternative locally produced reagent for molecular diagnostic applications.  
Analisis Variasi Genetik Gen L1 HPV (Human Papillomavirus) TIPE 16 Asal Sumatera Barat Andre Prima Rahmadhana; Marlina Marlina; Akmal Djamaan; Andani Eka Putra; Ifan Aulia Candra
Menara Ilmu : Jurnal Penelitian dan Kajian Ilmiah Vol 20, No 2 (2026): Vol 20 No. 02 APRIL 2026
Publisher : LPPM Universitas Muhammadiyah Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/mi.v20i2.7925

Abstract

Variasi nukleotida gen L1 terhadap data terkait di Gene Bank dilakukan dengan menggunakan 5 isolat DNA tipe 16 asal sumbar dimana setelah diamplifikasi hanya 2 yang berhasil di amplifikasi dan dilanjutkan dengan tahap sekuensing yakni sampel 3 dan sampel 4. Analisis dilakukan  menggunakan data sekuensing untuk kemudian dilakukan blast, konstruksi pohon filogenetik. Hasil analisis data sekuensing menunjukkan  sampel 3 memiliki kekerabatan dengan sekuen L1 di Gene Bank KU.9551191.1 dan sampel 4 memiliki kekerabatan dengan sekuen L1 JX313706.1. Kata Kunci: Gen L1, pohon filogenetik, primer BLAST, HPV 16, sekuensing.
Co-Authors - Johan Abdiana Abdiana, Abdiana Adang Bachtiar Ade Asyari Afida Razuna Ave Afrizal Agustian, Dede Rahman Aisyah Elliyanti Akmal Djamaan Alioes , Yustini Alponsin Alponsin Amelin, Fitrisia Amirah Zatil Izzah Amrin Alkamar Anavelda, Aura Putri Andre Prima Rahmadhana Annisa Widi Rizkia Antonius, Puja Agung Arfai Arfai Arfiandi Arfiandi Ariani, Tutty Arni Amir Arni Amir arniamir, Arni Amir Asri, Ennesta Bari'ah, Puti Asma Basyir, Vaulinne Besri, Hanifa Zahra Bobby Indra Utama Budi Junio Hermawan Cimi Ilmiawati, Cimi Darfioes Basir Deddy Saputra Dede Agustian Rahman Defni, - Defrin, Defrin Delfican Delfican Delfilaura, Asyifa Delmi Sulastri Densi Selpia Sopianti Densi Selpia Sopianti Desmawati Desmawati Dessy Arisanty Desy Nofita Sari Dia Rofinda, Zelly Dia Djanas, Dovy Dwitya Elvira Edison, Ebill Fuji Efrida Efrida Eka Fithra Elfi, Eka Fithra Elizabeth Bahar Elizabeth Bahar Elmatris Sy Elsa Alfiyanti Eryati Darwin Eti Yerizel Fadhil Aptana Sadjiana Fahma, Siskalil Farashanda, Meysha Farras, Umar Al Fathiyyatul Khaira Fauzul Azhim Febrianti Ika Kurnia Feby Resicha Fenty Anggraini Ferry Lismanto Syaiful Fika Tri Anggraini Finny Fitry Yani Fiona Dewanti Firdawati Firdawati Fitri Anggraini Fitri Anggraini, Fitri Fitri Nova, Fitri Fitri, Azdiana Fitrina, Dewi Wahyu Gardenia Akhyar Gestina Aliska Gestina Aliska Hafni Bachtiar Hanif, Monica Mulnia Hanif, Rayhendra Hardisman Hari, Dewi Gulyla Hasmiwati Haviz Yuad Haviz Yuad Helmizar Hidayanti, Rahmi Husna Yetti Husna, Annisa Tamara Husni Husni Ida Parwati Ifan Aulia Candra Ilmiawati, Ilmiawati Irrahmah, Miftah Irvan Medison Jamsari Jamsari Kaharudin Kaharudin Kaisar , Kevin Florentino Kosno Suprianto Leona, Denada Florencia Linosefa Linosefa Liza Fitria M. Alfian Agustian Mahata, Liganda Endo Malinda Meinapuri Maliza, Rita Marlina . Marlina Marlina Megariani Megariani Menkher Manjas Mentari Faisal Putri Meysha Farashanda Mila Permata Sari Monica Mulnia Hanif Muhammad Rayhan Firdaus Muhammad Reza Nelmi Silvia Netti Suharti Netti Suharti, Netti Nia Ayuni Putri Nila Kasuma Novita Ariani Nur Indrawaty Lipoeto Nurhayati, Nurhayati Oky Masir Prima Indra Puji Oktavia Putri Rizki Fitriani Putri, Mentari Faisal Rahmadian, Rizki Rahmat Syawqi Rahmi Lestari Rahmi, Wahida Ramadhan, Mario Arya Ranne Balqis RAVEINAL RAVEINAL Refi Amalia Utami Rheta Elkhaira Rinang Mariko Rinang Mariko Rita Hamdani Rizanda Machmud Rosfita Rasyid Roslaili Rasyid Roza Sri Yanti Russilawati, Russilawati Rustini Rustini Salmiah Agus Salmiah Agus Saptino Miro Sary, Rizka Yulia Sef Zani Meria Sekar Asri Tresnaningtyas silviayolanda, Silvia Yolanda Sinai, Nadiva Kezia Siskalil Fahma Sosmiarti Sosmiarti Sriyanti, Roza Suharti , Netti Suzana Devi Syahrul, Muhammad Zulfadli Syamel Muhammad Tarigan, Herri Novita Taufiqulhakim, Muhammad Rafi Tresnaningtyas, Sekar Asri Tyas, Syanindhita Wikanthining Utama, Bobby Indra Utami, Refi Amalia Vaulinne Basyir Wahyu Zikra Wenny Nursa Octarina Wiwik Andriani Yefri Zulfiqar Yessi Widya Putri Yosha Putri Wahyuni Yuad, Haviz Yufri Aldi Yufri Aldy Yuliati Shafan Nur Yuliati Shafan Nur Yusri Dianne Jurnalis