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All Journal Majalah Kedokteran Bandung Jurnal Kesehatan Andalas Sari Pediatri Jurnal Kesehatan Komunitas Journal of Maternal and Child Health JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Katalisator B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Buletin Ilmiah Nagari Membangun Oto Rhino Laryngologica Indonesiana Majalah Kedokteran Andalas Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Andalas obstetrics and gynecology journal Best Journal (Biology Education, Sains and Technology) Indonesian Journal of Health Sciences Research and Development (IJHSRD) Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks International Journal of Science and Society (IJSOC) Indonesian Journal of Multidisciplinary Science Jurnal Ilmu Kesehatan Indonesia (JIKSI) Andalas Dental Journal The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy International Journal of Advanced Science Computing and Engineering Jurnal Penyakit Dalam Indonesia Medika: Jurnal Ilmiah Kesehatan Jurnal Riset Ilmiah Vitalitas Medis : Jurnal Kesehatan Dan Kedokteran JIDAN (Jurnal Ilmiah Bidan) Stikes Pelita Ilmu Depok
Andani Eka Putra
Fakultas Farmasi Universitas Andalas
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The qRT-PCR in A Nasal Swab Leprosy Patient’s at Dr. M. Djamil Padang Hospital Ariani, Tutty; Asri, Ennesta; Putra, Andani Eka
International Journal of Science and Society Vol 6 No 3 (2024): International Journal of Science and Society (IJSOC)
Publisher : GoAcademica Research & Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54783/ijsoc.v6i3.1222

Abstract

Leprosy, caused by Mycobacterium leprae, in 2016, Indonesia reported 16,826 new leprosy cases, with a prevalence rate of 0.71 per 10,000 individuals and a new case detection rate of 6.5 per 10,000 individuals. This study describes the result of a quantitative real-time polymerase chain reaction (qRT-PCR) in the nasal mucosa smear of a leprosy patient at the Dermatology and Venereology Polyclinic of Dr. M. Djamil Padang Hospital. This study adopts an observational approach with a width cut design, specifically employing a cross-sectional study methodology on leprosy patient at the Dermatology and Venereology Polyclinic of Dr. M. Djamil Padang Hospital, Indonesia between February 2023 until February 2024. The qRT-PCR quantity value of nasal mucous discharge is analyzed by a real-time PCR machine. The provided data presents characteristics of a sample population (N = 36) with leprosy. The majority of the leprosy-affected individuals in this study are between 8 and 68 years old, with a median age of 34. According to the World Health Organization (WHO) classification indicates that 83.33% of cases are multibacillary (MB). A combination RLEP/ qRT-PCR assay was developed to enable laboratory-based care and follow-up of leprosy patients attending our outpatient polyclinic in M. Djamil, Padang. The assay offers a sensitive and precise method for assessing the bacterial load and viability of M. leprae from nasal swab samples. It can be used for treatment response monitoring and early diagnosis.
PROFIL KLINIS DAN BAKTERIOLOGIS INFEKSI SALURAN KEMIH PADA ANAK DI RSUP DR. M. DJAMIL PADANG TAHUN 2017 – 2021 Bari'ah, Puti Asma; Amelin, Fitrisia; Putra, Andani Eka; Izzah, Amirah Zatil; Welan, Rahmani
SINERGI : Jurnal Riset Ilmiah Vol. 2 No. 5 (2025): SINERGI : Jurnal Riset Ilmiah, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/sinergi.v2i5.1214

Abstract

Urinary Tract Infection (UTI) is the most common bacterial infection in children which is characterized by an increase in bacteria in the urine. The purpose of this study was to determine the clinical and bacteriological profiles of urinary tract infection patients in children at Dr. M. Djamil Padang in 2017-2021. This type of research is descriptive with a total sample method of 52 children with a retrospective approach on patient medical record data and urine culture results. The variables studied included age, gender, nutritional status, urinary tract abnormalities, clinical symptoms, physical examination, supporting examinations, and types of bacteria in pediatric urinary tract infection patients. The results of this study showed that most cases of UTI occurred in children aged 0-1 years (59,2%) with more male cases than females, good nutritional status (56,8%), with the most congenital abnormality in males is phimosis (37,9%). Based on physical examination, it was found that most of the patients did not have a fever (50%). The results of investigations showed that the patient had leukocyturia (61,5%) on urinalysis, leukocytosis (61,5%) on blood scan, cystitis (47.8%) on ultrasound, and nephrolithiasis (33%) on CT scan. Bacterial culture results show that the most common cause of UTIs in boys is Klebsiella pneumoniae (37.9%) and in girls Escherichia coli (87.0%). Based on this study, most results were obtained in the form of uncomplicated UTI. Gram-negative bacteria are the most common type of bacteria that cause UTIs.
Analysis of bile acid in saliva of patients with laryngopharyngeal reflux and non-laryngopharyngeal reflux Asyari, Ade; Utami, Refi Amalia; Yerizel, Eti; Putra, Andani Eka; Firdawati, Firdawati
Oto Rhino Laryngologica Indonesiana Vol. 53 No. 2 (2023): VOLUME 53, NO. 2 JULY - DECEMBER 2023
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v53i2.561

Abstract

ABSTRACT — Background: Laryngopharyngeal reflux (LPR) is the backflow of gastric and or duodenal fluid into the larynx, pharynx, trachea, and bronchi. The prevalence of LPR is difficult to determine due to the limited gold standard and the large variety of LPR symptoms. Damage can occur due to the decrease in pH value and also because of exposure to harmful enzymes in reflux, including bile acid. Purpose : This study was conducted to analyze bile acid levels in the saliva of LPR patient and non LPR subject. Methods:  This study is an observational study with a case-control design. The study was conducted in the ORL-HNS Department of Dr. M. Djamil Hospital, Padang, West Sumatra, Indonesia. The total sample size was 44 people. We enrolled 22 healthy subjects as the control group and 22 patients suspected of having LPR. Result: LPR patients are more common in women than in men, with 12 women and 10 men. Bile acid in the LPR group means of is 25.08±7.67µM, meanwhile, in the healthy group, the mean was 18.99±8.26 µM. There is a statistically significant in the incidence of LPR with the bile acids (p = 0.015) based on t- independent test. Conclusion: Our study confirmed that bile acids in saliva play a major role in diagnosing LPR. 
Perbandingan Metode Boiling Dan Kit Komersial Untuk Ekstraksi DNA TB, Tinjauan Dari Aspek Kualitas, Kuantitas, Dan Integritas DNA. Taufiqulhakim, Muhammad Rafi; Linosefa; Syahrul, Muhammad Zulfadli; Putra, Andani Eka
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 4 (2025): Desember 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i4.1399

Abstract

Latar Belakang: Tuberculosis (TB) merupakan suatu penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis. Prevalensi penyakit tuberkulosis pada tahun 2023 meningkat dari tahun 2022, hal ini juga disebabkan karena lamanya diagnosis dari penyakit tuberkulosis. Polymerase Chain Reaction (PCR) merupakan salah satu teknologi molekular yang berkembang untuk mendiagnosis penyakit tuberkulosis. Objektif: Penelitian ini bertujuan untuk menganalisis perbedaan ekstraksi DNA TB dengan menggunakan metode boiling dan kit komersial, yang ditinjau dari aspek kualitas, kunatitas, dan integritas DNA. Metode: Penelitian ini merupakan penelitian analitik. Penelitian ini dilakukan di Pusat Diagnostik dan Riset Penyakit Infeksi Fakultas Kedokteran Universitas Andalas Padang pada periode bulan Juli 2023 - Maret 2024. Hasil: Pada aspek kualitas DNA, ekstraksi DNA dengan menggunakan metode boiling memiliki rata-rata sekitar 1.47 dan kit komersial memiliki rata-rata sekitar 1.79. Pada aspek kuantitas DNA, ekstraksi DNA dengan menggunakan metode boiling memiliki rata-rata 7.1 ng/µl dan kit komersial memiliki rata-rata sekitar 17.1 ng/µl. Pada aspek integritas DNA metode boiling dapat memvisualisasikan 28 sampel positif  dan kit komersial 22 sampel positif. Kesimpulan: pada penelitian ini terdapat perbedaan yang signifikan antara metode boiling dan kit komersial pada aspek kualitas dan kuantitas DNA, dan tidak terdapat perbedaan yang signifikan antara metode boiling dan kit komersial pada aspek integritas DNA. Kata kunci:  Mycobacterium tuberculosis, Ekstraksi, DNA, Boiling, PCR  
CORRELATION BETWEEN FOOD QUALITY AND MICROBIOTA PROFILE OF PREGNANT WOMEN IN THE CITY OF PADANG : FOOD QUALITY AND MICROBIOTA PROFILE Sary, Rizka Yulia; Helmizar, Helmizar; Putra, Andani Eka
INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD) Vol. 7 No. 2 (2025): INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT
Publisher : STIKes Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36566/ijhsrd/Vol7.Iss2/316

Abstract

Background: Pregnancy is a complex physiological period that requires systemic adaptation of the body. During pregnancy, a balanced blood microbiota plays an important role in maintaining the mother's immune system and supporting fetal development. This study aims to determine the correlation between food quality and the microbiota profile of pregnant women in Padang City. Methods: This is an observational study with a cross-sectional design. The study was conducted from January to March 2025 at the Centre for Diagnostic and Research on Infectious Diseases (PDRPI) Laboratory, Andalas University. The sample size was 20 participants, selected using total sampling. Microbiota profiling was performed using 16S rRNA metagenomic sequencing. The results of the microbiota profile analysis were analyzed using Microbiome Analysist software. Results: Statistical analysis showed a p-value of 0.14702 and a t-statistic of -1.5182, indicating that there was no statistically significant difference between food quality and microbiota profile. The comparison of the Firmicutes/Bacteroidetes ratio (F/B ratio) was 1.06, indicating a balanced microbiota in pregnant women. Conclusion: Although not statistically significant, these differences in microbiota composition are biologically important and suggest a potential association between dietary patterns and systemic health in pregnant women.
Identification of Gender in Early Pregnancy Using Cell-Free Fetal Dna Hermawan, Budi Junio; Utama, Bobby Indra; Putra, Andani Eka
Andalas Obstetrics And Gynecology Journal Vol. 10 No. 1 (2026)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.10.1.135-144.2026

Abstract

Introduction: Traditionally, chorionic villus sampling and amniocentesis can be used to help identify fetal gender around 11 weeks of pregnancy. However, the risk of miscarriage with these procedures is approximately 1 to 3%. The discovery of cell-free fetal DNA (cffDNA) fragments in the plasma and serum of pregnant women carrying male fetuses has made reliable and accurate diagnosis using non-invasive procedures an option for determining fetal gender. To determine the success rate of fetal gender identification in early pregnancy using Cell Free Fetal DNA. Methods: An analytical observational research method to identify fetal gender in early pregnancy through Cell Free Fetal DNA examination in maternal blood. A total of 70 samples were collected using quota sampling technique. Data analysis was performed using sensitivity and specificity tests, as well as cross-sectional analysis to determine the relationship between PCR test results and gender at birth.      Results: The PCR test has low sensitivity (14.29%), meaning the test is less effective in correctly detecting male gender, resulting in many false negatives. However, it has high specificity (98.11%), indicating its excellent ability to detect female gender with very few false positives. Additionally, the Pearson Chi-Square statistical test resulted in a p-value of 0.033 (p < 0.05), indicating a statistically significant relationship between PCR test results and gender at birth.   Conclusion: Statistically, this test is better at identifying females than males, with a lower error rate for females.  
PENGEMBANGAN PETERNAKAN AYAM BROILER SEBAGAI ALTERNATIF PENINGKATAN EKONOMI DAN KETAHANAN PANGAN MASYARAKAT DI NAGARI SAKO PASIA TALANG KABUPATEN SOLOK SELATAN Sosmiarti Sosmiarti; Yuliati Shafan Nur; Arfai Arfai; Andani Eka Putra
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 8 No. 2 (2025)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v8i2.754

Abstract

In Nagari Sako Pasia Talang, Sungai Pagu District, South Solok Regency, the scarcity of accessible animal protein sources coupled with limited employment opportunities poses a considerable socio-economic challenge. This programme was designed to enhance household incomes and fortify food security through the structured development of broiler poultry farming. The implementation strategy encompassed community outreach via structured training sessions and hands-on workshops, alongside expert consultations facilitated by academics from Andalas University. An integrated educational and participatory approach was employed, incorporating technical training in broiler husbandry, the construction of standardized poultry housing, feed formulation utilizing locally available ingredients, livestock health management, and farm business financial analysis. Tangible support was also provided in the form of poultry housing units, day-old chicks (DOC), feed supplies, and essential farming equipment. The intervention yielded marked improvements in both the knowledge base and practical skills of participating community members, enabling the adoption of efficient, locally adaptive broiler farming techniques. A pilot, collectively managed poultry enterprise was successfully established, producing 970 market-ready broilers from an initial stock of 1,020 DOC, while achieving a competitive feed conversion ratio (FCR). Financial analysis revealed a potential net profit of IDR 9,131,160 per 35-day production cycle, underscoring the venture’s economic viability. Beyond income generation, the programme broadened community access to affordable and nutritious animal protein, and catalyzed the emergence of new, livestock-based entrepreneurial models. With its demonstrable success, the initiative holds significant potential for replication as a sustainable, village-centered strategy for economic resilience and food security. 
Co-Authors - Johan Abdiana Abdiana, Abdiana Adang Bachtiar Ade Asyari Afida Razuna Ave Afrizal Agustian, Dede Rahman Aisyah Elliyanti Akmal Djamaan Alioes , Yustini Amelin, Fitrisia Amirah Zatil Izzah Amrin Alkamar Anavelda, Aura Putri Annisa Widi Rizkia Antonius, Puja Agung Arfai Arfai Arfiandi Arfiandi Ariani, Tutty Arni Amir Arni Amir arniamir, Arni Amir Asri, Ennesta Bari'ah, Puti Asma Basyir, Vaulinne Besri, Hanifa Zahra Bobby Indra Utama Cimi Ilmiawati, Cimi Darfioes Basir Deddy Saputra Dede Agustian Rahman Defni, - Defrin, Defrin Delfican Delfican Delfilaura, Asyifa Delmi Sulastri Densi Selpia Sopianti Densi Selpia Sopianti Desmawati Desmawati Dessy Arisanty Desy Nofita Sari Djanas, Dovy Edison, Ebill Fuji Efrida Efrida Eka Fithra Elfi, Eka Fithra Elizabeth Bahar Elizabeth Bahar Elmatris Sy Eryati Darwin Eti Yerizel Fahma, Siskalil Farashanda, Meysha Farras, Umar Al Fathiyyatul Khaira Fauzul Azhim Febrianti Ika Kurnia Feby Resicha Fenty Anggraini Finny Fitry Yani Fiona Dewanti Firdawati, Firdawati Fitri Anggraini, Fitri Fitri Nova, Fitri Fitri, Azdiana Fitrina, Dewi Wahyu Gardenia Akhyar Gestina Aliska Hafni Bachtiar Hanif, Monica Mulnia Hanif, Rayhendra Hardisman Hari, Dewi Gulyla Hasmiwati Haviz Yuad Haviz Yuad Helmizar Hermawan, Budi Junio Hidayanti, Rahmi Husna Yetti Husna, Annisa Tamara Husni Husni Ida Parwati Ilmiawati, Ilmiawati Irrahmah, Miftah Irvan Medison Jamsari Jamsari Kaharudin Kaharudin Kaisar , Kevin Florentino Kosno Suprianto Leona, Denada Florencia Linosefa Linosefa Liza Fitria M. Alfian Agustian Mahata, Liganda Endo Malinda Meinapuri Marlina . Megariani Megariani Menkher Manjas Mentari Faisal Putri Mila Permata Sari Muhammad Rayhan Firdaus Muhammad Reza Nelmi Silvia Netti Suharti Netti Suharti, Netti Nia Ayuni Putri Nila Kasuma Novita Ariani Nur Indrawaty Lipoeto Nurhayati, Nurhayati Oky Masir Prima Indra Putri Rizki Fitriani Putri, Mentari Faisal Rahmadian, Rizki Rahmat Syawqi Rahmi Lestari Rahmi, Wahida Ramadhan, Mario Arya Ranne Balqis RAVEINAL RAVEINAL Rheta Elkhaira Rinang Mariko Rinang Mariko Rita Hamdani Rizanda Machmud Rosfita Rasyid Roslaili Rasyid Roza Sri Yanti Russilawati, Russilawati Rustini Rustini Salmiah Agus Salmiah Agus Saptino Miro Sary, Rizka Yulia Sef Zani Meria silviayolanda, Silvia Yolanda Sinai, Nadiva Kezia Sosmiarti Sosmiarti Sriyanti, Roza Suharti , Netti Suzana Devi Syahrul, Muhammad Zulfadli Syamel Muhammad Tarigan, Herri Novita Taufiqulhakim, Muhammad Rafi Tresnaningtyas, Sekar Asri Tri Anggraini, Fika Tyas, Syanindhita Wikanthining Utama, Bobby Indra Utami, Refi Amalia Wahyu Zikra Wahyuni, Yosha Putri Wenny Nursa Octarina Wiwik Andriani Yefri Zulfiqar Yessi Widya Putri Yuad, Haviz Yufri Aldi Yufri Aldy Yuliati Shafan Nur Yusri Dianne Jurnalis