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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kedokteran Brawijaya Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Narra J Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Journal of Fisheries & Marine Jurnal Agro Veteriner

Poedji Hastutiek

Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 278480

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Identification of Blood Parasite on Sacrifical Cattle Slaughtered during Idul Adha 1438 H in Surabaya City and Sidoarjo Regency Dhimar Maulud Dyahningrum; Mufasirin Mufasirin; Nenny Harijani; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to detect the presence of blood parasite that infects sacrificial cattle slaughtered during idul adha 1438 H in Surabaya City and Sidoarjo Regency. This research used 147 blood samples of sacrificial cattle and used two methods, those are thin blood smear stained with Giemsa 20% and Microhematocrit Centrifugation Technique. Based on the result of the examination using a microscope with 1000x magnification, there are positive samples infected with blood parasite, that consist of Babesia sp., and Anaplasma sp.

Prevalence and Nematode Infection Level on Gastrointestinal Tract at Horse (Equus caballus) in Bangkalan Madura Elok Apriliawati; Mufasirin Mufasirin; Wurlina Wurlina; Poedji Hastutiek; Lucia Tri Suwanti; Benjamin Christoffel Tehupuring
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to determine the prevalence, infection level, sex and age effect on the infection level of GIT Nematode parasite in a horse. The fecal samples analyzed using the methods of native, sedimentation, floatation, and worm eggs count per gram of feces. Sample examination found 54 positive infected gastrointestinal nematode parasite with prevalence rate of 87% (54 from 62). The prevalence of Trichonema sp., Strongylus sp., and Parascaris equorum are 37.1%, 16.1%, and 1.6%. There were also mixed infestation like Strongylus sp. and Trichonema sp; Strongylus sp. and Parascaris equorum; Trichonema sp. and Parascaris equorum with total prevalence 27.4%, 1.6%, and 3.2%. Sex and age of horse had a very significant and significant effect in prevalence and infection level of Nematode parasite.

Prevalence and Identification of Digestive Tract Protozoa in Goats in Labang District Bangkalan Regency Warda Nafalizza Efendi; Lucia Tri Suwanti; Abdul Samik; Poedji Hastutiek; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and to identify gastrointestinal protozoa in goats in Sub-district of Labang, Bangkalan Regency, Madura, Indonesia. The research was conducted in April - July 2019 with 100 samples of goat’s stool. Examination was done in the laboratory of Parasitology in Faculty of Veterinary Medicine, Airlangga University using direct smear, sedimentation and flotation methods. The result showed that goats in Labang, Bangkalan Regency were infected by protozoa Eimeria sp. and Blastocystis sp. and the prevalence was 84%. There were no significant differences in the age and sex of goats (p > 0.05).

Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 Î¼g/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.

Prevalensi Ektoparasit pada Kambing Kacang di Kecamatan Prambon Kabupaten Nganjuk Nining Sari Virgandina Vinola; Agus Sunarso; Nenny Harijani; Endang Suprihati; Poedji Hastutiek; Mufasirin Mufasirin
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research are to identify and prevalence of ectoparasite infestation in bean goats on the sub-district of Prambon, district of Nganjuk. The research was conducted from September until December 2019 with 115 samples of bean goats. The samples were identified in the Entomology and Protozoology Laboratory of Parasitology Department of the Veterinary Faculty of Airlangga University. This research use non-experimental method with survey study. This research found several kind of extoparasites that infestation bean goats, as follows: Bovicola caprae 24.3% the most infestation in dorsal region, Linognatus africanus 31.2% the most infestation in head-neck and dorsal region, Ctenocepallides felis 4.3%. Mix infestation found B. Caprae and L. Africanus 20.8%, B. Caprae and C. Felis 1%, B. Caprae, L. Africanus and C. Felis 1%. This ectoparasite usually found infestatio in head-neck, ventral, dorsal, inguinal, and ectremites. The prevalence of ectoparasites is 82.6% area at sub-district of Prambon, district of Nganjuk. The data analyzed using Chi-square test and Correspondence test. Statistically is no significant association (p>0.05) between the prevalence of ectoparasites that infested bean goats with types of stage and non-stage eclosure. This caused due to environmental conditions are dirty and many other livestock around the cage.

Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2 Toxocara Cati Diyah Ayu Candra; Nunuk Dyah Retno Lastuti; Nove Hidayati; Kusnoto Kusnoto; Poedji Hastutiek; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.

Efektifitas Anthelmintika Ekstrak Etanol Kulit Buah Delima (Punica granatum) terhadap Jumlah Kematian Cacing Ascaridia gallisecara inVitro Nila Murodah; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid; Setiawan Koesdarto; Rochmah Kurniasanti; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

This research was conducted to determine the anthelmintics effect of peel a pomegranate fruit extract etanol against Ascaridia galli worm in vitro. In this research, using 240 samples of A. galli with length 7-11cm without differentiating sex. The concentration of peel a pomegranate fruit extract etanol for immersing the Ascaridia galli were 10 mg/ml, 20 mg/ml, 40 mg/ml, and 80 mg/ml. Negative control was used PBS. Positif control was used piperazin citrate 10 mg/ml. Observation death A. galli worm and analysis of the data at the 6 hour, 12 hour, 18 hour, 24 hour, 30 hour and 36 hour using ANOVA test and Duncan Range Test. ANOVA result showed significant differences between treatments (p<0,05). Duncan Range Test result showed peel a pomegranate extract etanol 40 mg/ml had anthelmintics effects the 36 hour which comparable with piperazin citrate 10 mg/ml.

Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Agus Wijaya Agustono, Bodhi Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldi Hamdani Amelia Dwita Safitri Amirotul Azhimah Amirul Muslim Amrullah Ana Amaliah Anastasya, Jelita Arimbi Arimbi Arimbi Arimbi Arsin, Andi A. Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azzahra, Farah Nabila Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Budiarto Budiarto Choirul Oktavian Setiyadin Nayiron Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dewi Mahartina Dhimar Maulud Dyahningrum Dian Ayu Permatasari Diyah Ayu Candra Djoko Legowo Doohan Mahendra DWI PUTRI RAHMAWATI Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Ellza Agatha Damayanti Elok Apriliawati Emy Koestanti Sabdoningrum Endang Suprihati Endang Suprihati ERMA SAFITRI Fadila Zikri Amanda Fakhryyah Maharani Deviyanti Fania Selfiannisa Febrina Dian Permatasari Firda Shafa Salsabila Firman Hadi Fanani Gandul Atik Yuliani Gemini Alam Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hasanuddin Ishak Hasib, Abdullah Hayuning Nurrodhiya Heni Puspitasari Herry Agoes Hermadi Ihda Hanny, Khurun'In Fadia Indah Norma Triana, Indah Norma Indasari, Elly Nur Ira Sari Yudaniayanti Isra Wahid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Kartika Aditiya Amelia Kasman Kasman Khairullah, Aswin Rafif Kiki Amalia Rama Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Margaretha, Josephira Intan Mega, Toafan Satrio Melani Anggraini Meles, Dewa Ketut Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Moch. Rizky Darmawan Mochamad Lazuardi Moh. Ilham Rizkul Ulum Mohamad Safri Sauqi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Hambal Muhammad Hambal Muhammad Rasyid Ridha Muhammad Ridwan NASHIHATUS SHOLIHAH Nenny Harijani Ngizzah, Nailul Nila Murodah Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nove Hidayati Nunuk Dyah Retno Lastuti Nusdianto Triakoso Panjaitan, Cahaya Permata Sari, Dian Ayu Permatasari, Dian Ayu Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Prawasa, Ramadhiniyanti Putri Alif Primarizky, Hardany Puput Ade Wahyuningtyas Rahaju Ernawati Rahmi Sugihartuti Ramadhanty, Miladhiyah Nabila Ratna Damayanti Ratu Meidiza Retno Bijanti Retno Wulansari Ridwan Amiruddin Ririn Ririn Riwu, Katty Hendriana Priscilia Rochmah Kurniasanti Rochmah Kurnijasanti Rohmah, Miftahur Rohmah, Siti Nur Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sardjana, I Komang Wiarsa Sari, Aulia Puspita Setiawan Koesdarto Sherbina Bai Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sudirman Nasir Suherni Susilowati Sulaeman, Nur Sa'adah Sunarso, Agus Suroiyah, Fifi Anik Suryanie Sarudji Talita Yuanda Reksa Tamaulina Br Sembiring Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Utami, Kinanti Putri Venti Safitri Warda Nafalizza Efendi Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widodo, Oky Setyo Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yustinasari, Lita Rakhma

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