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All Journal Jurnal Veteriner Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Medik Veteriner Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology GUYUB: Journal of Community Engagement Mitra Mahajana: Jurnal Pengabdian Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat
Tri Wahyu Suprayogi
Study Program Of Veterinary Paramedic, Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia
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The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

Abstract

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.
Spermatozoa motility and viability of turkey (Meleagris gallopavo) semen in egg yolk nanoparticles-phosphate buffered saline extender at 3-5°C storage Nabila Ayu Saraswati; Herry Agoes Hermadi; Tri Wahyu Suprayogi; Tatik Hernawati; Eka Pramyrtha Hestianah
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.129-138

Abstract

This study aimed to evaluate the spermatozoa motility and viability of turkey semen extended with egg yolk nanoparticles-PBS extender at various concentrations, stored at 3-5°C. Semen was collected from two turkeys and divided into four treatment groups. Group T0 consisted of fresh turkey semen extended with 20% (v/v) fresh egg yolk in phosphate-buffered saline (PBS). Groups T1, T2, and T3 consisted of fresh turkey semen extended with 5, 10, and 20% (v/v) egg yolk nanoparticles in PBS, respectively. Extended semen was stored in a refrigerator (3-5°C), and progressive spermatozoa motility and viability were evaluated every two hours until spermatozoa motility decreased to 40%. The results showed that turkey semen in T3 extender containing 20% ​​egg yolk nanoparticles in PBS was able to maintain spermatozoa motility for up to eight hours, better than T0 and T2 extenders which supported spermatozoa motility for six hours. In contrast, T1 extenders only support spermatozoa motility for four hours. T3 extenders were also able to maintain spermatozoa viability for up to eight hours, while T0, T1 and T2 extenders could only support viability for 6 hours. The extender with 20% egg yolk nanoparticles in PBS was significantly better (p <0.05) in maintaining spermatozoa quality compared to those with 5 and 10% egg yolk nanoparticles, as well as the extender with 20% fresh egg yolk. In conclusion, 20% egg yolk nanoparticles in PBS semen extender was effective in maintaining the motility and viability of turkey (Meleagris gallopavo) spermatozoa stored at 3-5°C for up to eight hours.
Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Ethanolic extract of Dayak onion (Eleutherine palmifolia) prevented sperm membran damage in mice exposed to monosodium glutamate Syahraini Adhiya Lathifah; Aldin Akbar Rahmatullah; Boedi Setiawan; Chairul Anwar Nidom; Nove Hidajati; Sri Mulyati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.153-160

Abstract

Monosodium glutamate (MSG) could cause increased production of reactive oxygen species (ROS), causing oxidative stress in the testicles, which adversely affected sperm quality. Dayak onion extract which is known for its high antioxidant content, could help alleviate oxidative stress caused by MSG. This research examined the effect of Dayak onion (Eleutherine palmifolia) on sperm abnormalities and plasma membrane integrity in mice (Mus musculus) exposed to MSG. Twenty-five male mice, 8 weeks old with a body weight approximately 20g, were divided into five groups, C– (received 0.5% Na-CMC), C+ (received 4 mg/g bw MSG), and three treatment groups (T1, T2, T3) which were given 4 mg/g bw MSG accompanied by Dayak onion extract at doses of 30, 60, and 120 mg/kg bw respectively. All treatments lasted for 52 days. Significant differences (p <0.05) in sperm abnormalities and plasma membrane integrity were observed among the groups. Sperm abnormalities found were (12.3 ± 1.92), (61.1 ± 3.10), (41.6 ± 2.87), (30.4± 1.91), and (18.2 ± 2.10) % respectively for C–, C+, T1, T2 and T3. Meanwhile, sperm plasma membrane integrity found were (33.0 ± 3.24), (69.3 ± 2.32), (41.8 ± 2.42), (55.4 ± 3.11), and (64.2 ± 1.27) % respectively for C–, C+, T1, T2 and T3. These results indicate that Dayak onion extract could help reduce sperm abnormalities and maintain plasma membrane integrity in mice exposed to MSG.
PEMBERDAYAAAN PETERNAKAN KAMBING POTE MELALUI TEKNIK KAWIN SUNTIK UNTUK PENINGKATAN EKONOMI MASYARAKAT BANGKALAN Sosilowati, Suherni; Hernawati, Tatik; Suprayogi, Tri Wahyu; Candika, Yossy Imam; Supriyadi, Supriyadi
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 5 No. 3 (2024): Volume 5 Nomor 3 Tahun 2024
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v5i3.4556

Abstract

Community Service was conducted to increase local people’s economy by Poté goat population using Artificial Insemination in Alaskokon, Modung, and Bangkalan. The partner in this Community Service are local Poté goat farmers in Alaskokon village, Modung, Bangkalan. The analysis of this situation shows a problem that the Partner has been facing. Even though the Poté population is not lacking, their rearing is conventional where it is not focused on selling but more on saving for when there is no money (Rojokoyo). To tackle this problem, Artificial Insemination was introduced to the Poté. Moreover, socialisation was done by covering various aspects. Sosialisation was given on caring for pregnant does, semen collection using an artificial vagina, and buck semen dilution using different types of extenders such as coconut water, milk, and egg yolk-citrate. Socialisation of the marketing strategy for goats was also given. Artificial Insemination Technology is a solution for improving the goat population. Goats are fast-bred livestock with a relatively short gestation time and can utilise forage like grasses. Artificial Insemination technique can be administered three times in two years to increase the goat population, thus, the local people’s economy may follow. After the programme was completed, farmers were enthusiastic about collecting goat semen and inseminating livestock.
Efisiensi Produksi Melalui Penerapan Teknologi Reproduksi Inseminasi Buatan Pada Kawin Silang Kambing Poteh Di Desa Baipajung Kecamatan Tanah Merah Kabupaten Bangkalan Susilowati, Suherni; Mustofa, Imam; Wahyu Suprayogi, Tri; Supriyadi; Mulyati, Sri
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2024): Vol. 6 No. 2 (2024) : Desember 2024
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v6i2.372

Abstract

Baipajung is a village with quite good potential value and needs to be developed in the agriculture, plantation and livestock sectors. Generally, in the village goats are mated naturally, namely one cage is used for 1 male goat and 3 female goats. The population of goats in Baipajung village is 488 male goats, 878 female goats and 587 goat kids, so with the livestock capital it can be used as a source of community income. The problem that occurs in Baipajung village, although the goat population is quite large, their maintenance is only traditional, just a side business as rojokoyo or as savings if they don't have money. Goat farmers want their goats after giving birth to have superior characteristics such as increased percentage of offspring, heavier weaning weight, higher quality goats or more efficient use of feed resources. The efficiency of livestock production can be increased through the application of Artificial Insemination reproductive technology in crossbreeding Poteh or local goats with Boar goat studs.
COUNSELING ON REPRODUCTIVE DISORDERS MANAGEMENT IN FEMALE BEEF CATTLE LIVESTOCK GROUPS IN DALEGAN VILLAGE, PANCENG DISTRICT, GRESIK REGENCY Suprayogi, Tri Wahyu; Susilowati, Suherni; Hermadi, Herry Agoes; Al Arif, M Anam; Hernawati, Tatik; Mulyati, Sri; Suwanti, Lucia Tri; Maslachah, Lilik; Kurnijasanti, Rochmah; Lestari, Tita Damayanti; Effendi, Mustofa Helmi; Hernanda, Ary Setya; Supriyadi; Saputra, Rakan Mahiid; Dewi, Aprilia Kurnia
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.026-038

Abstract

Management of reproductive disorders in female beef cattle (Bos taurus) is a vital step in enhancing livestock productivity and animal welfare. This educational activity was conducted in Dalegan Village, Panceng District, Gresik Regency, aiming to assist farmers in improving cattle reproductive performance through feed technology and supporting the development of modern farming practices. The methods applied included counseling, practical field demonstrations, and interactive discussions with participants, presented in a qualitative descriptive format. The results indicated an improvement in farmers' knowledge of the causes, clinical signs, and management steps for reproductive disorders such as anestrus, dystocia, and endometritis, as evaluated through pre-test and post-test assessments. Additionally, the activity provided guidance on the importance of feed management, cattle reproductive health, and modern farm management. In conclusion, this counseling successfully increased farmers' awareness of cattle reproductive health, which is expected to support the productivity of beef cattle farming in the region.
Prevalence of Feline Scabies at Puskeswan Bangsalsari, Jember During 2021-2023 Rifky Nugroho; Epy M. Luqman; Tri Wahyu Suprayogi; Suharti Aryanata; Suhita Aryaloka; Aswin Rafif Khairullah
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.282-289

Abstract

Feline Scabies is a highly contagious and zoonotic skin disease. It can be transmitted from animals to humans and from person to person. This research was carried out at Puskeswan Bangsalsari, Jember, East Java. Data were collected regarding the number of patients from 2021 to 2023 and organized monthly by the occurrence of scabies in cats. The prevalence of feline scabies at Puskeswan Bangsalsari, Jember, in 2021 was 31.29%, based on 133 scabies cases out of 425 total patients. In 2022, the prevalence of feline scabies was 33.21%, based on 186 cases out of 560 total patients. In 2023, the prevalence increased to 38.53%, with 210 cases recorded from a total of 545 patients. The cumulative prevalence of feline scabies at Puskeswan Bangsalsari, Jember, from 2021 to 2023, was 34.74%, based on 529 scabies cases out of a total of 1,520 patients. The highest prevalence of feline scabies was observed in August 2023, reaching 56.71%, with 21 cases out of 37 total patients. This may have been caused by high humidity, which could affect cage hygiene and create favorable conditions for Sarcoptes scabiei to complete its life cycle.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Co-Authors A.T Soelih Estoepangestie AA Sudharmawan, AA Abdul Samik Abrian Panggalih Indra Pratama Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Al Arif, M Anam Aldin Akbar Rahmatullah Alfiutha Propha Bhaskara Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Andrika Indra Pratomo Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Arinda octavia Asih Deskayanti Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Benjamin Chr. Tehupuring Boedi Setiawan Brahmantya, Chandra BUDI UTOMO Budi Utomo Budi Utomo Candika, Yossy Imam Dadik Rahardjo Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Dinda Reisinta Djoko Legowo Djoko Legowo Dwi Novita Sari Eduardus Bimo Aksono Herupradoto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestianah Epy M. Luqman Erma Safitri Erma Safitri Fajar Ramadhan Subiyantoro Febby Fairy Brillianti Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidayatik, Nanik Himatul Oktavia I. Ismudiono IMAM MUSTOFA Imam Mustofa Indah Norma Triana Indah Norma Triana Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Itrah Khoirunnisa Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kadek Rachmawati Khuliyah Candraning Diyanah Kukuh Prastyaningtyas Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lilik Maslachah Linda Listyorini Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Mas’ud Hariadi Mas’ud Hariadi Meles, Dewa Ketut Melsa Netika Mirni Lamid Mirza Asafaizh Arini Mufasirin Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nidom, Chairul Anwar Norma Amalia Ulfah Nove Hidajati Nusdianto Triakoso Oky Susandani Palupi, Tantri Dyah Whidi Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Pristafania, Triana Pudji Srianto R.T. Santanu Adikara Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Restiadi, Tjuk Imam Retno Sri Wahjuni Ricko Nurdianto S. Rifky Nugroho Rimayanti Rimayanti Rochmah Kurnijasanti Rochmi, Siti Eliana Roesno Darsono Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Sena Sangga Renata Septiana Alafia Yusi Ardiyansyah Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suherni Susilowati Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Sunaryo Hadi Warsito Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Aulia Ristiani Widya Paramita L Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yossy Imam Candika Yustisiane Ruth Rahadi