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All Journal Jurnal Veteriner Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Medik Veteriner Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology GUYUB: Journal of Community Engagement Mitra Mahajana: Jurnal Pengabdian Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat

Tri Wahyu Suprayogi

Study Program Of Veterinary Paramedic, Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia

Author-ID : 2866056

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Pengaruh Perbedaan Waktu Ekuilibrasi Sebelum Pembekuan Terhadap Kualitas Spermatozoa Sapi Rambon Banyuwangi Menggunakan Pengencer Tris Kuning Telur Setyawan, Firman; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Restiadi, Tjuk Imam; Saputro, Amung Logam; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan waktu ekuilibrasi 2 jam, 3 jam, 4 jam dan 5 jam terhadap motilitas, viabilitas, dan abnormalitas spermatozoa Sapi Rambon sebelum pembekuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan yaitu waktu ekuilibrasi P1 (2 jam), P2 (3 jam), P3 (4 jam) dan P4 (5 jam), satu ekor pejantan sapi rambon, dan lima kali pengulangan. Hasil analisis statistik Anova satu arah dan dilanjut uji Duncan mendapatkan hasil motilitas (P1) 76.00±2.2/3; (P2) 73.00±2.7/3; (P3) 67.00±2.7/3; dan (P4) 52.00±2.7/2. Hasil viabilitas (P1) 86.00±1.41; (P2) 78.00±2.73; (P3) 72.00±5.70 dan (P4) 57.00±2.73. Hasil abnormalitas (P1) 3.7±0.75; (P2) 3.8±0.75; (P3) 4.4±1.08 dan (P4) 5.2±0.57. Kesimpulan dari penelitian ini menunjukan hasil yang berbeda nyata (p<0.05) antara waktu ekuilibrasi dengan kualitas spermatozoa Sapi Rambon sebelum pembekuan dan hasil dari penelitian ini menunjukan persentase motilititas tertinggi terdapat pada waktu ekuilibrasi 2 jam, persentase viabilitas tertinggi terdapat pada waktu ekuilibrasi 2 jam dan persentase abnormalitas terendah terdapat pada waktu ekuilibrasi 2 jam.

Perbandingan Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Terhadap Persentase Motilitas, Viabilitas dan Integritas Membran Plasma Spermatozoa Kambing Sapera pada Penyimpanan Suhu 5ºC Iskandari, Novia Nur; Madyawati, Sri Pantja; Wibawati, Prima Ayu; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.196-202

Penelitian ini bertujuan untuk mengetahui perbedaan pengencer tris kuning telur dan susu skim kuning telur terhadap persentase motilitas, viabilitas dan integritas membran plasma spermatozoa kambing Sapera. Metode penelitian dengan mengumpulkan semen, pemeriksaan kelayakan semen segar, pengenceran semen, penyimpanan suhu 5ËšC dan pemeriksaan setiap 24 jam. Data diolah menggunakan anova faktorial dengan membandingkan antara dua bahan pengencer terhadap waktu pemeriksaan. Data menunjukkan tris kuning telur signifikan (p<0.05) terhadap susu skim kuning telur. Lama penyimpanan menunjukkan hasil signifikan pada hari ke-1 aspek motilitas 84.44% : 80%, viabilitas 89.67% : 86% dan integritas membran plasma 83.67% : 79.33%. Tris kuning telur dan susu skim kuning telur menunjukkan adanya interaksi (p<0.05 ) terhadap waktu pemeriksaan motilitas namun tidak terdapat interaksi (p>0.05) terhadap waktu pemeriksaan viabilitas dan integritas membran plasma. Disimpulkan pengencer tris kuning telur dan susu skim kuning telur berpengaruh pada persentase motilitas, viabilitas dan integritas membran plasma spermatozoa.

Honey Can Increase The Length of The Small Intestinal Villi in Malnourished Albino Rats Listyorini, Linda; Mustofa, Imam; Hernawati, Tatik; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Safitri, Erma -
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.175-179

The aim of this study was to determine the potential of honey to the length of small intestine villi of albino rats due to nutritional deficiencies. The experimental animals were used 24 male albino rats with a body weight of 250-300 grams, divided into four groups and each group contained six replication. Negative control (K-) was a group of rat without fasting and without honey. Positive control (K +) was a group of rat with fasting conditions for 5 days and without honey. The treatment group was the rat with fasting conditions for 5 days and given honey therapy at a dose of 30% (P1) and 50% (P2). Data analysis used ANOVA (Analysis of Variants) then continued with the Duncan Multiple Range test with a significance level of 5% it can be seen that the length of small intestine villi was obtained at (K-) which is 531,321 ± 0,64 which is not significantly different (p>0,05) from (P2), which is 519,919 ± 0,49, but is significantly different (p<0,05) with other treatments (K+) 360,564 ± 0,87 and (P1) 434,275 ± 0,40. The conclusion of this study is that giving honey has the potential to increase the length of the intestinal villi of albino rats due nutritional deficiencies.

Suppementation of Kelor Leaf (Moringa oleifera) Aqueous Extract Increase on Post-Thawed Limousin Bull Sperm Quality Wajdi, Syuhuud Arumbinang; Utomo, Budi; Rimayanti, Rimayanti; Safitri, Erma; Suprayogi, Tri Wahyu; Wurlina, Wurlina
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.249-255

The purpose of this research was to determine the best dosage of Moringa oleifera Aqueous extract in egg yolk skim milk extender for post thawed Limousin Bull sperm quality on aspect viability, and the level of. The treatment was divided into five groups: egg yolk and skim milk diluter (P0), 2,5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P1), 5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P2), 10% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P3), 20% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P4). The sperm quality was observed post thawing. The data were analyzed using ANOVA and Duncant Test. The best sperm motility showed on P2 with 43b ± 5,70, the best sperm viability showed on P3 with 58,20b ± 8,72 and than the lowest level of malondialdehyde showed on P4 with 5,434a ± 1,034. In conclusion addition of M. oleifera on dose 10% can increase quality of Limousin Sperm Post Thawed.

Medical Treatment for Pyometra in Cat Palupi, Tantri Dyah Whidi; Suprayogi, Tri Wahyu; Ismudiono, Ismudiono
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.124-130

The Pyometra case is a case that is often found in Female Cat. This case report will discuss the handling of a case of pyometra in a 7-year-old mixed breed cat who is being treated at Harmoni Pet Care, Menanggal, Gayungan, Surabaya, Indonesia. Reports from the owner that these cats are still in good appetite but sanguino-purulent discharge from the vagina was found. In clinically diagnosed with a pyometra, the treatment used to treat pyometra case was ovariohysterectomy. Postoperative care was carried out by administering antibiotics (Clanexi; Amoxicillin-Clavulanic Acid, 1 cc/kg, IM) given twice a day for 14 days, with the first 3 days being treated in the Harmoni Pet Care inpatient room and the 4th day to the 14th day of care is done at the owner's house, local antiseptic dressing at the incision site is continued for up to 14 days until the sutures in the skin sutures were absorbed by the cat's body.

Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing Wijayanti, Ani; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Hernawati, Tatik; Sardjito, Trilas; Saputro, Amung Logam; Amaliya, Anny; Sulistyowati, Deny
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.66-74

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle.

Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences Amrullah, Muhammad Fajar; Utomo, Budi; Utama, Suzanita; Suprayogi, Tri Wahyu; Lestari, Tita Damayanti; Restiadi, Tjuk Imam; Belgania, Ristaqul Husna
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.125-131

The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.

POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 Î¼g/kg BW, P1 group of groups exposed to TCDD doses of 7 Î¼g / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7Î¼g/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7Î¼g/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.

Prevalensi Parasit Saluran Pencernaan pada Kerbau (Bubalus bubalis) Melalui Pemeriksaan Feses di Kabupaten Agam Sumatra Barat Ridwan, Muhammad; Suwanti, Lucia Tri; Suprayogi, Tri Wahyu; Mufasirin, Mufasirin; Kusnoto, Kusnoto; Hastutiek, Poedji
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

ABSTRAKPenelitian ini bertujuan untuk mengetahui jenis, prevalensi, dan pengaruh jenis kelamin serta umur terhadap prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Provinsi Sumatra Barat. Sampel yang digunakan dalam penelitian ini adalah feses dari 105 ekor kerbau yang dikumpulkan dari tiga Kecamatan. Pemeriksaan sampel dilakukan dengan menggunakan metode natif, sedimentasi, dan pengapungan. Penelitian ini memberikan hasil bahwa ditemukan infeksi saluran pencernaan pada kerbau berupa cacing Paramphistomum sp. 31,43%, Fasciola sp. 23,81%, Oesophagustomum sp. 1,9%, Bunostomum sp. 0,95%, Strongyloides sp. 0,95%, Trichostrongylus sp. 0,95%, dan protozoa Eimeria sp. 2,86%. Prevalensi yang diperoleh sebesar 45,7% (48/105). Hasil analisis statistik menunjukkan bahwa jenis kelamin dan umur tidak mempengaruhi prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Sumatra Barat.Keywords: cacing dan protozoa, kerbau, prevalensi, saluran pencernaanPrevalence of Gastrointestinal Parasite in Buffalo (Bubalus bubalis) Through Feces Examination in Agam Regency West SumatraABSTRACTThis study aims to determine the type, prevalence, and influence of sex and age on the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra Province. The samples used in this study were feces from 105 buffaloes collected from three Districts. Examination of the sample is carried out using by direct smear methods, sedimentation, and flotation. This study provides results that found gastrointestinal infections in buffalo by helminth Paramphistomum sp. 31.43%, Fasciola sp. 23.81%, Oesophagustomum sp. 1.9%, Bunostomum sp. 0.95%, Strongyloides sp. 0.95%, Trichostrongylus sp. 0.95%, and protozoa Eimeria sp. 2,86%. Prevalence is 45.7% (48/105). The results of statistical analysis showed that sex and age did not affect the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra.Keywords: buffalo, gastrointestinal, helmint and protozoa, prevalence

The addition of duck egg yolk in skim milk – fructose extender maintains sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage Isti Annisa Ramahtia; Tatik Hernawati; Tri Wahyu Suprayogi; Tjuk Imam Restiadi; Djoko Legowo; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.1-8

This study aimed to determine the addition of duck egg yolk in skim milk – fructose extender on sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage. Ejaculates of Muscovy duck were divided into four portions equally. The first portion was for the control group (diluted in skim milk – fructose extender), and the second, third, and fourth portions were for the treatment groups of T1, T2, and T3 (diluted in skim milk – fructose extender added with 2.5, 5.0, and 7.5% v/v duck egg yolk). Sperm motility and viability were evaluated two hourly until the motility percentage reached 40%. The data were analyzed using ANOVA and Duncan test. The result showed that the highest percentage of spermatozoa motility and viability (p<0.05) was in the T2 group compared to the other groups. It could be concluded that the Muscovy duck semen extended in skim milk - fructose with 2.5 to 7.5% egg yolk of duck qualified for artificial insemination at storage up to 10 hours in 5°C storage.

Co-Authors Abdul Samik Abdul Samik, Abdul Adikara, R.T. Santanu Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad, Agung Budianto Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Aksono HP., Eduardus Bimo Al Arif, M Anam Aldin Akbar Rahmatullah Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Anas Bad’rosana Prawindi Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Ardiyansyah, Septiana Alafia Yusi Arini, Mirza Asafaizh Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Bhaskara, Alfiutha Propha Boedi Setiawan Brahmantya, Chandra Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Candika, Yossy Imam Dadik Rahardjo, Dadik Darsono, Roesno Deskayanti, Asih Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Djoko Legowo Djoko Legowo Dwi Novita Sari Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Epy M. Luqman Erma Safitri Estoepangestie, A.T Soelih Fajar Ramadhan Subiyantoro Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Himatul Oktavia Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khoirunnisa, Itrah Khuliyah Candraning Diyanah Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Meles, Dewa Ketut Mirni Lamid Mufasirin Muhammad Ikmal Robih Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Muhammad Yunus Mustofa Helmi Effendi Nabila Ayu Saraswati Netika, Melsa Nidom, Chairul Anwar Nove Hidajati Nurdianto S., Ricko Nusdianto Triakoso octavia, Arinda Oky Susandani Palupi, Tantri Dyah Whidi Paramita L, Widya Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Pratomo, Andrika Indra Pristafania, Triana Pudji Srianto Rahadi, Yustisiane Ruth Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Reisinta, Dinda Renata, Sena Sangga Retno Sri Wahjuni Rifky Nugroho Rimayanti Rimayanti Ristiani, Widya Aulia Rochmah Kurnijasanti Rochmi, Siti Eliana Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tehupuring, Benjamin Chr. Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Tsaniya Atikahani Shabirah Ulfah, Norma Amalia Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti

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