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Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda Widya Aulia Ristiani; Muhammad Yunus; Tri Wahyu Suprayogi; Pudji Srianto; Imam Mustofa; Rimayanti Rimayanti
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.153 KB) | DOI: 10.20473/ovz.v9i1.2020.12-16

Abstract

This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center.  Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.
The effect of red dragon fruit skin extract (hylocereus polyrhizus) on the number of leydig cells, diameter of seminiferous tubules, and testicular weight of male mice (mus musculus) exposured with hot temperatures Kukuh Prastyaningtyas; Rochmah Kurnijasanti; Rahmi Sugihartuti; Suherni Susilowati; Tri Wahyu Suprayogi; Hana Eliyani
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.18-24

Abstract

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.
Potensi madu pada peningkatan jumlah sel spermatogenik tikus (rattus norvegicus) yang kekurangan nutrisi Linda Listyorini; Tatik Hernawati; tri wahyu suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.12-17

Abstract

This study aimed to determine honey's potential in increasing the number of spermatogenic cells (spermatogonium, spermatocyte and spermatid) of rats (Rattus norvegicus) with nutritional deficiencies. Twenty four male rats (Rattus norvegicus) were randomly divided into four groups. Negative control (K-) mice was not fasted nor given honey. Positive control (K+) mice was fasted for 5 days without the administration of honey. The treatment group rats were fasted for 5 days followed by administration of honey 30% (P1) and 50% (P2) in drinking water for 10 days. On the 76th day all rats were sacrificed and their testes were collected for histological slides using Hematoxylin-Eosin staining. The results showed that fasting treatment for five days (group K +) caused a decrease (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to those of the normal mice (group K–). Administration of 50% honey (group P2) for 10 days caused an increase (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to the K + group mice. In P2 group, the number of spermatogonia cells was lower (p <0.05), while the number of spermatocytes and spermatids was not significantly different than in the K– group mice. It could be concluded that the administration of honey was able to regenerate the testicular tissue of rats with nutritional deficiency by increasing the number of spermatogenic cells in the seminiferous tubules.
The effect of watermelon (citrullus lanatus) rind ethanolic extract on the number of leydig, sertoli, and spermatogenic cells of rat (rattus novergicus) exposed to heat Abrian Panggalih Indra Pratama; Suherni Susilowati; Lilik Maslachah; Hermin Ratnani; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.7-11

Abstract

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p >0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p <0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.
Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas Arinda octavia; Budi Utomo; Sri Pantja Madyawati; Tri Wahyu Suprayogi; Agus Sunarso; Iwan Sahrial Hamid
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.65-71

Abstract

 This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.
Kualitas semen sapi pejantan berdasarkan umur, suhu, dan kelembaban di Taman Ternak Pendidikan Universitas Airlangga Febby Fairy Brillianti; Pudji Srianto; Trilas Sardjito; Tri Wahyu Suprayogi; Indah Norma Triana; Dadik Rahardjo
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.81-89

Abstract

This research was conducted in the teaching farm of the Faculty of Veterinary Medicine, Universitas Airlangga. The present study aimed to determine the effect of age, temperature, and humidity on bull semen quality which was processed into frozen semen. Three age groups of bulls (young 3-4 years, middle age 5-6 years, and old 7-8 years) were collected for the temperature and humidity of semen examination. Fresh semen was collected using an artificial vagina, followed by macroscopic and microscopic examination. In addition, post-thawing motility was also evaluated. The results showed that there was no effect of temperature and humidity during semen collection process. Fresh semen quality was affected by age. Moreover, the post-thawing motility of fresh semen of spermatozoa was lower than those of the fresh semen; however, it still qualifies for artificial insemination. It could be concluded that the semen of bulls at the Teaching Farm of Universitas Airlangga has good quality, and is suitable for Artificial Insemination.
Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows Norma Amalia Ulfah; Abdul Samik; Mas’ud Hariadi; Tri Wahyu Suprayogi; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.9-14

Abstract

Small farmers dominate dairy farming in Indonesia, and the average productivity is low. The contribution of domestic production only reaches about 23% of the total national milk consumption needs as a source of human nutrition. This study was aimed to identify the relationship between cow's parity and  age to services per conception (S/C), conception rate (CR), and gestation length (GL) of Holstein Friesian (HF) cross dairy cows. Data of this study consisted of primary data obtained from farmers interview, and secondary data from the Cooperative Unit of Argopuro village, Krucil, Probolinggo. Sample size was 200 cows, which was 10% of the total population. Data were analyzed for correlation and linear regression using the Statistic Package and Service Solution (SPSS) software version 20 (IBM Corp., USA) at a 95% confidence level. The average S/C, CR, and GL were 2.55 ± 1.57, 21%, and 278.19 ± 11.92 days, respectively. There was no significant correlation (p >0.05) between the parity with S/C, CR, and GL and the age with CR and GL. The only significant correlation (p <0.05) was between the age and S/C. Regression of S/C based on age followed the equation S/C = 0.104 + 0.407*age, with a correlation coefficient and determination coefficient of 0.733 and 0.538, respectively. It could be concluded that S/C could be predicted based on the age of the cows.
The Potency of Giving Turmeric (Curcuma domestica Val.) Flour to The Quality of Quail (Coturnix coturnix japonica) Eggs Astrid Annisya Adhy Putri; Agus Widodo; Ratna Damayanti; Tri Wahyu Suprayogi
Journal of Applied Veterinary Science And Technology Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I1.2020.1-5

Abstract

Background: Supplementation of good feed on quail can improve its egg quality. One of the excellent supplement comes from herbs. Turmeric is one of the herbs which good for livestock because of its content, curcuminoid, and essential oil. Curcuminoid can increase quail egg yolk color, increase the appetite so that it can increase the weight of poultry. Purpose: To know the potential of giving turmeric flour to the quality of quail’s egg. Methods: The methods used 40 quails, divide into two groups. Groups P0: quail fed without turmeric flour, and group P1: quails fed with 1,07% turmeric fluor. Variables observed were egg weight, haugh unit, egg yolk index, white egg index, and yolk color. Result: The Egg weight, haugh unit, yolk index, and yolk color of P1 was higher than quails fed without turmeric. However, there was no difference between the egg whites index of both groups. Conclusion: The addition of turmeric on feed has the potential to increase the egg weight, haugh unit, yolk index, and yolk color. However, turmeric was not directly affected the formation of egg whites. 
The Incidence of Gastrointestinal Helminthiasis in Etawa Crossbred Goat in Etawa Farm Jombang Dwi Novita Sari; Tri Wahyu Suprayogi; Djoko Legowo; Siti Eliana Rochmi
Journal of Applied Veterinary Science And Technology Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I1.2020.24-28

Abstract

Background: Goat is one type of small ruminant that has a contribution to supporting the development of livestock and is an alternative to meet the needs of animal protein in Indonesian society. The current goat raising system is still carried out by being caged or semi-grazed. In this maintenance system, goats are very susceptible to parasitic diseases, especially those caused by the digestive tract worms. Gastrointestinal worms are a significant problem in livestock, especially small ruminants that cause health problems, such as diarrhea, weight loss, and decreased appetite. Purpose: To determine the percentage and types of digestive tract helminths in Etawa crossbred goats by age. Method: 30 feces samples from Etawa crossbred goats were collected for laboratory examination using native, sedimentation, and flotation methods. Result: From the microscopic examination, it was found that the prevalence of gastrointestinal helminthiasis was 56.6%. Also, the type of worm eggs found in the feces samples from Etawa crossbred goats were Cotylophoron cotylophorum, Moniezia benedeni, Oesophagostomum columbianum, Strongyloides papillosus, Haemonchus contortus, Trichostrongylus sp., and Trichuris globulosa.
Weight Gain and Feed Conversion of Broiler Chickens in Reviewed from Cage Temperature and Humidity Himatul Oktavia; Siti Eliana Rochmi; Tri Wahyu Suprayogi; Djoko Legowo
Journal of Applied Veterinary Science And Technology Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V2.I1.2021.5-9

Abstract

Background: Broiler chicken production is influenced by several factors, namely genetic, management, and environmental factors. Genetically superior chickens will not produce maximum results if not supported by a comfortable environment for chickens (comfort zone) because they are animals that will grow optimally at a certain temperature range. in addition, humidity also affects the production and health of chickens. So that the selection of the cage becomes important to create comfortable environmental conditions for chickens. Cage with a relatively stable temperature will greatly affect the production results, including body weight and feed conversion. Purpose: To determine the effect of temperature and humidity of semi-enclosed cages on weight gain and feed conversion of broiler chicken. Method: Observation was carried out during a period of maintenance of broiler chickens on farms and data collected in the form of the recording of chicken weight and feed conversion. Result: Temperature and humidity of semi-enclosed cages have a good effect on production results, with chicken harvest weight of 2197.3 grams and feed conversion of 1.52 kg. Conclusion: this study indicate that temperature and humidity have a good influence on weight gain of chickens and feed conversion value.
Co-Authors A.T Soelih Estoepangestie AA Sudharmawan, AA Abdul Samik Abrian Panggalih Indra Pratama Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Al Arif, M Anam Aldin Akbar Rahmatullah Alfiutha Propha Bhaskara Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Andrika Indra Pratomo Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Arinda octavia Asih Deskayanti Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Benjamin Chr. Tehupuring Boedi Setiawan Brahmantya, Chandra BUDI UTOMO Budi Utomo Budi Utomo Candika, Yossy Imam Dadik Rahardjo Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Dinda Reisinta Djoko Legowo Djoko Legowo Dwi Novita Sari Eduardus Bimo Aksono Herupradoto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestianah Epy M. Luqman Erma Safitri Erma Safitri Fajar Ramadhan Subiyantoro Febby Fairy Brillianti Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidayatik, Nanik Himatul Oktavia I. Ismudiono IMAM MUSTOFA Imam Mustofa Indah Norma Triana Indah Norma Triana Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Itrah Khoirunnisa Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kadek Rachmawati Khuliyah Candraning Diyanah Kukuh Prastyaningtyas Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lilik Maslachah Linda Listyorini Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Mas’ud Hariadi Mas’ud Hariadi Meles, Dewa Ketut Melsa Netika Mirni Lamid Mirza Asafaizh Arini Mufasirin Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nidom, Chairul Anwar Norma Amalia Ulfah Nove Hidajati Nusdianto Triakoso Oky Susandani Palupi, Tantri Dyah Whidi Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Pristafania, Triana Pudji Srianto R.T. Santanu Adikara Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Restiadi, Tjuk Imam Retno Sri Wahjuni Ricko Nurdianto S. Rifky Nugroho Rimayanti Rimayanti Rochmah Kurnijasanti Rochmi, Siti Eliana Roesno Darsono Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Sena Sangga Renata Septiana Alafia Yusi Ardiyansyah Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suherni Susilowati Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Sunaryo Hadi Warsito Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Aulia Ristiani Widya Paramita L Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yossy Imam Candika Yustisiane Ruth Rahadi