Claim Missing Document
Check
Articles

Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus Susilowati, Suherni; Tri Wahyu Suprayogi; Tatik Hernawati; Yossy Imam Candika; Widya Paramita Lokapirnasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.18-24

Abstract

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.
Reproductive Performance of Crossbreed Cows on Lowland Terrain Ardiyansyah, Septiana Alafia Yusi; Sri Agus Sudjarwo; Nusdianto Triakoso; Tri Wahyu Suprayogi; Ismudiono Ismudiono; Oky Setyo Widodo
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.124-130

Abstract

This study aims to identify the differences in reproduction performance of Ongole cross (PO), Limousine cross (Limpo), and Simmental cross (Simpo) in Kerek district, Tuban regency. Primary data was taken by interviewing inseminators and farmers, while secondary data was taken from the Fisheries and Animal Husbandry Department of Tuban regency. The total sample was 360 cows consisting of 30 PO, Limpo, and Simpo cows aged 3, 4, 5, and 6 years respectively which were calculated based on the Roscoe formula. Data was analyzed using the Simple Linear Regression and ANOVA followed by Duncan's multiple range test at a significance level of 95%. The results showed that the services per conception (S/C), conception rate (CR), and calving rate (CvR) were not significantly different (p >0.05) between PO, Limpo and Simpo cows. Meanwhile, days open (DO) and fertility status (FS) of PO cows were higher (p <0.05) than Limpo and Simpo cows. There was no significant difference (p >0.05) in DO and FS between Limpo and Simpo cows. The linear equation with age as a predictor (X) of S/C, DO, and CI (Y) values were not significant (p >0.05) in PO, Limpo, and Simpo cows. It could be concluded that the reproductive performance of PO cows was better than Limpo and Simpo cows.
TRAINING ON ANIMAL FEED PROCESSING AND TREATMENT OF REPRODUCTIVE DISORDERS IN PALM INTEGRATED CATTLE FARMING AREA IN WEST KALIMANTAN Hidayatik, Nanik; Lamid, Mirni; Hermadi, Herry Agoes; Warsito, Sunaryo Hadi; Suprayogi, Tri Wahyu; Safitri, Erma; Pratomo, Andrika Indra
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 1 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i1.2024.114-120

Abstract

West Kalimantan Province is one of the provinces that has a large area of oil palm plantations which can become integrated cattle farms. Oil palm plantations provide a source of feed that comes from palm oil processing waste and green grass around the oil palm trees. However, this waste contained not sufficient nutrients if given directly, so needed feed processing to increase the nutrition. The community service team from the Faculty of Veterinary Medicine, Universitas Airlangga carried out activities to increase the knowledge and skills of farmers in processing palm oil waste feed and carried out reproductive examination activities on female cattle. The output target to be achieved is increasing the ability of farmers to make feed from palm oil waste that can sufficient for livestock nutritional needs and increased of reproductive health of cows. This activity held on 20 – 23 July 2023 in Tonang Village, District. Sengah Temila, Kab. Landak, West Kalimantan. The activity was attended by 40 participants consisting of breeders, medical officers and veterinarians from the Dinas Ketahanan Pangan, Pertanian dan Perikanan Kabupaten Landak dan Dinas Perkebunan dan Peternakan Provinsi Kalimantan Barat. The first day's activities were carried out by delivering outreach and training materials on processing palm oil waste feed through silage and fermentation methods, making UMB and UMMB, and carrying out reproductive examinations and treatment of female cattle. The outcomes that have been achieved include increasing farmers' understanding and skills regarding how to make silage and palm frond fermentation, as well as UMB and UMMB as additional feed. Health and reproductive disorders examination of female cattle were carried out on 2 female cattle belonging to the Koperasi Produsen Landak Mandiri and 17 female cattle belonging to the Alam Raya Hijau Group. Based on the examination, data was obtained that 7 cows were pregnant, 1 cow had endometritis, and 11 cows were declared to be in normal reproductive condition. The conclusion that can be drawn from this activity is that the participants' knowledge about the given topic increases and the reproductive health of female cattle can be handled with appropriate examination and treatment.
Success rate of artificial insemination using sexed semen in dairy cows at the Setia Kawan dairy farmers cooperative, Pasuruan, Indonesia Tsaniya Atikahani Shabirah; Tri Wahyu Suprayogi; Tita Damayanti Lestari; Tatik Hernawati; Rimayanti Rimayanti; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.112-118

Abstract

This study aimed to evaluate the success rate of artificial insemination (AI) using sexed semen in dairy cows, based on the parameters of services per conception (S/C), conception rate (CR), and calf sex ratio. A descriptive method was employed, utilizing both primary and secondary data. Primary data were collected through structured interviews with farmers using questionnaires, while secondary data were obtained from institutional records of the Setia Kawan dairy farmers cooperative, located in Tutur District, Pasuruan Regency, East Java, Indonesia. A total sample of 250 dairy cows was selected, representing 15% of the 1,669 cows that had undergone AI with sexed semen. All sampled cows had at least one prior calving and were owned by members of the cooperative, with AI performed between 2021 and 2022. Artificial insemination was conducted by certified inseminators with over five years of experience, within 6-12 hours of estrus detection. The results showed an S/C value of 1.308 (327 inseminations for 250 cows) and a CR of 81.60% (204 pregnancies from 250 inseminations). Of the 250 calves born, 85.2% (213) were female and 14.8% (37) were male, resulting in a female-to-male sex ratio of 5.76:1. These findings suggest that AI using sexed semen can significantly improve both reproductive efficiency and the production of female offspring in dairy herds.
Potential of Basil (Ocimum basilicum) leaf extract in Tris-egg yolk extender on sperm motility and plasma membrane integrity of Sapudi ram at room temperature Pratama Putra Banusiandana; Suherni Susilowati; Sri Mulyati; Tri Wahyu Suprayogi; Emy Koestanti Sabdoningrum; Nove Hidajati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.119-126

Abstract

This study aimed to determine the effect of basil (Ocimum basilicum) leaf extract in a Tris-egg yolk extender on the motility, and plasma membrane integrity of Sapudi ram spermatozoa at room temperature. Fresh semen samples were collected from Sapudi ram using an artificial vagina. A completely randomized design was used, consisting of four treatment groups with five replicates each. Data were analyzed using SPPS with factorial analysis of variance (ANOVA) and Duncan’s test. The control group (T0) received Tris-egg yolk extender without basil leaf extract. Treatment groups T1, T2, and T3 received 1%, 2%, and 3% basil leaf extract, respectively, in the extender. Results showed that sperm motility and plasma membrane integrity (Table 4) tended to decrease with increased storage time. Among the treatment groups, the addition of 1% basil leaf extract in the Tris-egg yolk extender (T1) resulted in significantly higher (p <0.05) both sperm motility and plasma membrane integrity compared to the control (T0). However, higher concentrations of basil leaf extract (T2 and T3) did not result in significant differences (p >0.05) in sperm motility after three hours of storage compared to the 1% treatment (T1). In conclusion, the addition of 1% basil leaf extract to a Tris-egg yolk extender was effective in maintaining sperm motility and plasma membrane integrity of Sapudi ram spermatozoa stored at room temperature for up to three hours.
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
Co-Authors Abdul Samik Abdul Samik, Abdul Adikara, R.T. Santanu Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad, Agung Budianto Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Aksono HP., Eduardus Bimo Al Arif, M Anam Aldin Akbar Rahmatullah Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Anas Bad’rosana Prawindi Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Ardiyansyah, Septiana Alafia Yusi Arini, Mirza Asafaizh Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Bhaskara, Alfiutha Propha Boedi Setiawan Brahmantya, Chandra Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Candika, Yossy Imam Dadik Rahardjo, Dadik Darsono, Roesno Deskayanti, Asih Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Djoko Legowo Djoko Legowo Dwi Novita Sari Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Epy M. Luqman Erma Safitri Estoepangestie, A.T Soelih Fajar Ramadhan Subiyantoro Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Himatul Oktavia IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khoirunnisa, Itrah Khuliyah Candraning Diyanah Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Meles, Dewa Ketut Mirni Lamid Mufasirin Muhammad Ikmal Robih Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Muhammad Yunus Mustofa Helmi Effendi Nabila Ayu Saraswati Netika, Melsa Nidom, Chairul Anwar Nove Hidajati Nurdianto S., Ricko Nusdianto Triakoso octavia, Arinda Oky Susandani Palupi, Tantri Dyah Whidi Paramita L, Widya Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Pratomo, Andrika Indra Pristafania, Triana Pudji Srianto Rahadi, Yustisiane Ruth Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Reisinta, Dinda Renata, Sena Sangga Retno Sri Wahjuni Rifky Nugroho Rimayanti Rimayanti Ristiani, Widya Aulia Rochmah Kurnijasanti Rochmi, Siti Eliana Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tehupuring, Benjamin Chr. Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Tsaniya Atikahani Shabirah Ulfah, Norma Amalia Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti