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Success rate of artificial insemination using sexed semen in dairy cows at the Setia Kawan dairy farmers cooperative, Pasuruan, Indonesia Tsaniya Atikahani Shabirah; Tri Wahyu Suprayogi; Tita Damayanti Lestari; Tatik Hernawati; Rimayanti Rimayanti; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.112-118

Abstract

This study aimed to evaluate the success rate of artificial insemination (AI) using sexed semen in dairy cows, based on the parameters of services per conception (S/C), conception rate (CR), and calf sex ratio. A descriptive method was employed, utilizing both primary and secondary data. Primary data were collected through structured interviews with farmers using questionnaires, while secondary data were obtained from institutional records of the Setia Kawan dairy farmers cooperative, located in Tutur District, Pasuruan Regency, East Java, Indonesia. A total sample of 250 dairy cows was selected, representing 15% of the 1,669 cows that had undergone AI with sexed semen. All sampled cows had at least one prior calving and were owned by members of the cooperative, with AI performed between 2021 and 2022. Artificial insemination was conducted by certified inseminators with over five years of experience, within 6-12 hours of estrus detection. The results showed an S/C value of 1.308 (327 inseminations for 250 cows) and a CR of 81.60% (204 pregnancies from 250 inseminations). Of the 250 calves born, 85.2% (213) were female and 14.8% (37) were male, resulting in a female-to-male sex ratio of 5.76:1. These findings suggest that AI using sexed semen can significantly improve both reproductive efficiency and the production of female offspring in dairy herds.
Potential of Basil (Ocimum basilicum) leaf extract in Tris-egg yolk extender on sperm motility and plasma membrane integrity of Sapudi ram at room temperature Pratama Putra Banusiandana; Suherni Susilowati; Sri Mulyati; Tri Wahyu Suprayogi; Emy Koestanti Sabdoningrum; Nove Hidajati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.119-126

Abstract

This study aimed to determine the effect of basil (Ocimum basilicum) leaf extract in a Tris-egg yolk extender on the motility, and plasma membrane integrity of Sapudi ram spermatozoa at room temperature. Fresh semen samples were collected from Sapudi ram using an artificial vagina. A completely randomized design was used, consisting of four treatment groups with five replicates each. Data were analyzed using SPPS with factorial analysis of variance (ANOVA) and Duncan’s test. The control group (T0) received Tris-egg yolk extender without basil leaf extract. Treatment groups T1, T2, and T3 received 1%, 2%, and 3% basil leaf extract, respectively, in the extender. Results showed that sperm motility and plasma membrane integrity (Table 4) tended to decrease with increased storage time. Among the treatment groups, the addition of 1% basil leaf extract in the Tris-egg yolk extender (T1) resulted in significantly higher (p <0.05) both sperm motility and plasma membrane integrity compared to the control (T0). However, higher concentrations of basil leaf extract (T2 and T3) did not result in significant differences (p >0.05) in sperm motility after three hours of storage compared to the 1% treatment (T1). In conclusion, the addition of 1% basil leaf extract to a Tris-egg yolk extender was effective in maintaining sperm motility and plasma membrane integrity of Sapudi ram spermatozoa stored at room temperature for up to three hours.
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
PROTECTIVE EFFECTS OF COMBINED VITAMIN E AND ZINC SULFATE ON OVARIAN AND UTERUS WEIGHT OF ALBINO RATS (Rattus norvegicus) EXPOSED TO LEAD ACETATE Fitriani Sultan; Tri Wahyu Suprayogi; Rimayanti; Ragil Angga Prastiya; Bodhi Agustono; Amung Logam Saputro
VITEK : Bidang Kedokteran Hewan Vol. 16 No. 1 (2026): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/594dbr52

Abstract

Lead exposure exerts toxic effects on the female reproductive system, particularly on the ovaries and uterus, resulting in hypofunction and organ atrophy. Oxidative stress induced by lead accumulation may be attenuated by antioxidants such as vitamin E and zinc sulfate. This study aimed to evaluate the protective effects of vitamin E, zinc sulfate, and their combination on ovarian and uterus weights in albino rats (Rattus norvegicus) exposed to lead acetate. Twenty-five female rats were randomly divided into five groups (K, T0, T1, T2, and T3) and treated orally for 21 days. On day 22, the rats were euthanized, and the ovaries and uterus were collected and weighed. The average ovarian weights for K, T0, T1, T2, and T3 were 25.80 ± 2.49; 18.62 ± 4.36; 28.40 ± 5.67; 33.78 ± 6.09; and 36.84 ± 5.15 mg, respectively. The average uterus weights for K, T0, T1, T2, and T3 were 292.48 ± 36.81; 249.96 ± 25.97; 315.86 ± 26.56; 325.54 ± 31.53; and 375.00 ± 36.55 mg, respectively. In conclusion, vitamin E and zinc sulfate demonstrated protective effects against lead-induced reproductive toxicity, with the combined treatment showing the greatest improvement in ovarian and uterus weights.
Determination of puberty based on morphometric measurements and the gonadosomatic index of lovebird (Agapornis fischeri) testes Nabila Chusnannada; Yeni Dhamayanti; Boedi Setiawan; Sunaryo Hadi Warsito; Tri Wahyu Suprayogi; Kuncoro Puguh Santoso
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.34-43

Abstract

This study aimed to determine the relationship between age and testicular morphometrics in lovebird (Agapornis fischeri), as well as the relationship between age and the gonadosomatic index (GSI) of the testes, and to evaluate the effect of body weight on testicular weight in determining the onset of puberty. This study used 30 lovebirds divided into three age groups: 4, 5, and 6 months, with ten birds in each group. The variables measured were testicular length, width, volume, weight and body weight. Data were analyzed using correlation analysis, simple linier regression, and paired sample t-test. The results showed a positive relationship between age and testicular length, width and volume. The GSI values of lovebirds aged 4, 5 and 6 months were 0.015 ± 0.002, 0.058 ± 0.011, and 0.400 ± 0.067, respectively, indicating an increase with age. The significance coefficient at 4 months of age was 0.000 (p <0.05), indicating that body weight had a significant influence on testicular weight at this age. In contrast, lovebirds aged 5 and 6 months had significance coefficients of 0.079 and 0.089 (p >0.05), indicating that testicular weight was not significantly influenced by body weight at these ages. It can be concluded that lovebirds at 6 months of age are likely to have reached puberty.
Pengembangan Seni Hidangan Alternatif dan Terinspirasi pada Kader Posyandu Balita Sesuai Kandungan Gizi Isi Piringku di Desa Karangsono Kabupaten Jember Saskia Nadhira Ayudani; Khuliyah Candraning Diyanah; Aditya Sukma Sukma Pawitra; Nur Rahma Febriani; F. Himmatul Aliyah; Ardan Saputra; Na&#039;ilah Putri Az&#039;zahroh; Muhammad Ilham Rofiudin; Triana Pristafania; Akbar Maulana Azhari; Nabila Berlianny Nur Anisa; Ria Lusiandari; Tri Wahyu Suprayogi
GUYUB: Journal of Community Engagement Vol 5, No 1 (2024)
Publisher : Universitas Nurul Jadid

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33650/guyub.v5i1.8012

Abstract

Stunting poses a serious health issue in Indonesia. In 2022, the national prevalence of stunting stood at 22.6%, exceeding the World Health Organization's requirement of below 20%. According to the Indonesian Nutrition Status Survey (SSGI) 2022 by the Ministry of Health, Jember Regency has the highest prevalence of stunted toddlers in East Java, reaching 34.9% or approximately 35,000 toddlers. To address the stunting problem, health efforts are being made through the prevention of stunting diseases with the implementation of the "SEHATI" (Seni Hidangan Alternatif dan Terinspirasi or Alternative or Inspired Food Art) program in Karangsono Village, Bangsalsari Subdistrict, Jember Regency. This program involves community health volunteers managing meals according to the "Isi Piringku" guidelines with appropriate nutritional content for toddlers. The aim of this activity is to raise awareness and knowledge among community health volunteers about the importance of managing balanced and nutritious meals according to the "Isi Piringku" program issued by the Ministry of Health. This study uses a Pre-Experimental Design method with a Pretest-Posttest design model to measure the increase in knowledge of community health volunteers after socialization about the "Isi Piringku" program. The research results show that 90% of volunteers experienced an average knowledge increase of 13.5 after the counseling activities. The "SEHATI" program successfully stimulates children's appetite through unique and appealing foods with balanced nutritional value. The "SEHATI" program is expected to serve as an example for the community in preparing balanced and nutritious meals for children. Through accurate and easily understandable nutrition education, it is hoped that awareness of the importance of healthy and nutritious food intake will increase, ultimately aiding in the prevention of stunting and other nutritional problems in Jember Regency. 
Efisiensi Produksi Melalui Penerapan Teknologi Reproduksi Inseminasi Buatan Pada Kawin Silang Kambing Poteh Di Desa Baipajung Kecamatan Tanah Merah Kabupaten Bangkalan Suherni Susilowati; Imam Mustofa; Tri Wahyu Suprayogi; Supriyadi; Sri Mulyati
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2024): Desember 2024
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v6i2.372

Abstract

Baipajung is a village with quite good potential value and needs to be developed in the agriculture, plantation and livestock sectors. Generally, in the village goats are mated naturally, namely one cage is used for 1 male goat and 3 female goats. The population of goats in Baipajung village is 488 male goats, 878 female goats and 587 goat kids, so with the livestock capital it can be used as a source of community income. The problem that occurs in Baipajung village, although the goat population is quite large, their maintenance is only traditional, just a side business as rojokoyo or as savings if they don't have money. Goat farmers want their goats after giving birth to have superior characteristics such as increased percentage of offspring, heavier weaning weight, higher quality goats or more efficient use of feed resources. The efficiency of livestock production can be increased through the application of Artificial Insemination reproductive technology in crossbreeding Poteh or local goats with Boar goat studs.
Co-Authors Abdul Samik Abdul Samik, Abdul Adikara, R.T. Santanu Aditya Irawan Aditya Sukma Sukma Pawitra Agung Budianto Achmad, Agung Budianto Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Akbar Maulana Azhari Aksono HP., Eduardus Bimo Al Arif, M Anam Aldin Akbar Rahmatullah Amaliya, Anny Amrullah, Muhammad Fajar Anas Bad’rosana Prawindi Ani Wijayanti Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardan Saputra Ardina Sahra Miranda Ardiyansyah, Septiana Alafia Yusi Arini, Mirza Asafaizh Astrid Annisya Adhy Putri Aswin Rafif Khairullah Belgania, Ristaqul Husna Bhaskara, Alfiutha Propha Boedi Setiawan Brahmantya, Chandra Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Candika, Yossy Imam Dadik Rahardjo, Dadik Darsono, Roesno Deskayanti, Asih Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Djoko Legowo Djoko Legowo Dwi Novita Sari Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Epy M. Luqman Epy Muhammad Luqman Erma Safitri Estoepangestie, A.T Soelih F. Himmatul Aliyah Fajar Ramadhan Subiyantoro Fitriani Sultan Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Himatul Oktavia Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Khoirunnisa, Itrah Khuliyah Candraning Diyanah Kuncoro Puguh Santoso Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Masyitoh, Herlina Mas’ud Hariadi Meles, Dewa Ketut Mirni Lamid Mufasirin Muhammad Ikmal Robih Muhammad Ilham Rofiudin Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Muhammad Yunus Mustofa Helmi Effendi Na&#039;ilah Putri Az&#039;zahroh Nabila Ayu Saraswati Nabila Berlianny Nur Anisa Nabila Chusnannada Netika, Melsa Nidom, Chairul Anwar Nove Hidajati Nur Rahma Febriani Nurdianto S., Ricko Nusdianto Triakoso octavia, Arinda Oky Susandani Paramita L, Widya Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Pratomo, Andrika Indra Pudji Srianto Rahadi, Yustisiane Ruth Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Reisinta, Dinda Renata, Sena Sangga Retno Sri Wahjuni Ria Lusiandari Rifky Nugroho Rimayanti Rimayanti Rimayanti Ristiani, Widya Aulia Rochmah Kurnijasanti Rochmi, Siti Eliana Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Saskia Nadhira Ayudani Seagames Waluyo, Seagames Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Suharti Aryanata Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Supriyadi Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tantri Dyah Whidi Palupi Tatik Hernawati Tehupuring, Benjamin Chr. Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Triana Pristafania Trilas Sardjito Tsaniya Atikahani Shabirah Ulfah, Norma Amalia Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti