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Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle Winni Liani Daulay; Putri Indah Ningtias; Cece Sumantri; Jakaria Jakaria
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.69784

Abstract

Investigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 genotype was found in BB x PO F1 cattle and BB x PO F2 cattle. The +/+ genotype, which showed a normal phenotype was only detected in PO cattle. There was a significant difference of the MSTN gene expression in the sampled animals among genotypes and between phenotypes (normal-muscled vs double muscled). The MSTN expression in animals with del.11/del.11 genotype was higher than that in animals with +/del11 and +/+ genotypes (P<0.05). Animals with +/+ genotype showed the lowest MSTN expression. It was concluded that double-muscled animals showed higher MSTN expression than normal-muscled animals.
Performa Pertumbuhan dan Produksi Karkas Mandalung Hasil Hibridisasi antara Entok Jantan (Cairina moschata) dengan Itik Betina (Anas plathyrynchos) (The Growth Performance and Production of Carcass Mandalung, Hybridized from Male Muscovy Ducks (Cairina moschata) and Female Ducks (Anas plathyrynchos)) Danang Priyambodo; Jakaria Jakaria; Rukmiasih Rukmiasih
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 5 No. 1 (2015): Jurnal Sains Terapan, Volume 5, Nomor 1, Tahun 2015
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.491 KB) | DOI: 10.29244/jstsv.5.1.8-16

Abstract

Crossbreeding between species (hybridization) was conducted to obtain higher average value of offspring trait than the parent trait. The aim of this study was to determine the best type of mule duck and to evaluate the heterosis effect appearing on mule duck obtained. The experimental design used was a randomized block design with two levels of treatment and eight groups based on the hatching period. The treatments were the type of mule duck crossed between muscovy duck male with female cihateup duck (EC) and mule duck crossed between male muscovy duck with female alabio duck (EA). The results showed the growth performance and carcass production of EC and EA mule duck were not significantly different, but mule duck EC tended to show better results than mule duck EA. The percentage of heterosis effect produced by EC and EA mule duck in growth performance and carcass production showed advantages over the parent. EC mule duck had a higher level of heterosis than EA mule duck for some trait observed. EC mule duck can be kept as best crossbreed to produce mule duck with good performance and carcass production.Keywords: mule duck, performance, carcass production, heterosis
Sensori dan Karakteristik Asam Lemak Daging Itik Cihateup, Alabio dan Silangannya (Sensory and Fatty Acids Characteristics of Cihateup Duck Meats, Alabio and Cross-bred) Fitriani Eka Puji Lestari; Jakaria Jakaria; Rukmiasih Rukmiasih
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 5 No. 1 (2015): Jurnal Sains Terapan, Volume 5, Nomor 1, Tahun 2015
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.256 KB) | DOI: 10.29244/jstsv.5.1.17-25

Abstract

The purpose of this study was to evaluate the off odor in the meat of Alabio, Cihateup and cross-bred of both on the second generation. There were 36 ducks consisting 8 Cihateup Ducks, 9 alabio ducks and 19 cross-bred ducks. These ducks were reared for 10 weeks and fed by commercial feed containing 21-23% protein and 2900-3000 kcal/kg metabolic energy as much as 125 g/head/day. Ducks were put in colonies in box sized 1m x1 m. The off odor intensity, degree of liking and fatty acid composition of chest and thighs meat from Cihateup Duck, Alabio Duck and their crossbreeding were determined. Therefore, off odor intensity was analyzed using the rating intensity. Degree of liking was performed by hedonic ratio fatty acid composition analysis which was conducted by Gas Chromatography (GC). The off odor intensity of commercial meet of Alabio, Cihateup and their crossbreeding were not significantly different. Degree of aroma liking among Alabio, Cihateup and their crossbreeding meat was significantly different in breast commercial cutting where their cross breeding meat had the highest degree of liking. Ratio of unsaturated fatty acid to saturated fatty acid of meat from crossbreeding duck was lower than the one from the ancestor.Keyword: sensory, duck meat, Alabio, Cihateup, cross-breed
Keragaman Fenotipe Sifat Kualitatif Ayam Burgo di Provinsi Bengkulu T. Rafian; Jakaria Jakaria; N. Ulupi
Jurnal Sain Peternakan Indonesia Vol 12, No 1 (2017)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.12.1.47-54

Abstract

Penelitian ini bertujuan untuk mengidentifikasi keragaman sifat kualitatif ayam burgo ayam hutan merah (Gallus gallus gallus), dan ayam kampung (Gallus gallus domesticus). Jumlah ayam ayam menggunakan adalah 74 ekor yang terdiri atas ayam burgo 47 ekor, ayam hutan merah (Gallus gallus gallus) 9 ekor, dan ayam kampung (Gallus gallus domestica)18 ekor yang dikoleksi di Bengkulu Selatan, Kota Bengkulu, Bengkulu Utara, dan Muko-muko, Provinsi Bengkulu. Karakteristik sifat kualitatif yang diamati adalah warna bulu, corak bulu, pola bulu, kerlip bulu, warna shank, tipe jengger, warna cuping, dan warna mata. Data sifat kualitatif berupa frekuensi fenotipe, frekuensi gen, nilai heterozigositas, nilai introgresi, dan gen asli. Ayam burgo dan ayam hutan merah memiliki keragaman yang rendah pada sifat kualitatifnya, sedangkan ayam kampung memiliki keragaman yang tinggi pada sifat kualitatifnya. Berdasarkan hasil penelitian, ayam burgo perlu dipertahankan dan dikembangkan sebagai sumber daya genetik ayam lokal Indonesia di Bengkulu.Kata kunci: Ayam burgo, ayam hutan merah, ayam kampung, sifat kualitatif
Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep A. Gunawan; D. Anggrela; K. Listyarini; M. A. Abuzahra; Jakaria Jakaria; M. Yamin; I. Inounu; C. Sumantri
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.335 KB) | DOI: 10.5398/tasj.2018.41.3.165

Abstract

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.
The Prediction of Prolificacy Using Linear Body Parameters and Craniometric Analysis in Etawah-Grade Does R. H. Mulyono; C. Sumantri; R. R. Noor; Jakaria Jakaria; D. A. Astuti
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.281 KB) | DOI: 10.5398/tasj.2018.41.2.77

Abstract

Etawah-grade goat is a graded  up line between Kacang and Etawah goats, which  well adapted to Indonesia’s humid tropical climate. In order to fulfill national meat requirement, it is necessary to increase the  population by increasing prolificacy. This study was aimed to determine whether the body and head measurements of the Etawah-grade does can be used as selection criteria for  indirect selection of the litter size. The use of does at 3-4 years old (I3 dental condition) from BPTU-HPT Pelaihari and Cordero farms (51 and 55 does, respectively) were based on their specific geographical location  characteristics. The body variables were withers height, hip height, body length, chest width, chest depth, thurl width, rump length, chest girth, and cannon circumference, whereas for head variables were acrocranion–prosthion, basion–prosthion, lower jaw length, head height, tuber facial left-right, nasion–rhinion, entorbitale left-right, euryon left-right, supraorbitale left-right. Bergmann methods  were applied for measuring the differences of body and head parameters followed by the principal component, Fisher’s discriminant, and principal component regression analyzes. All regression coefficients of linear body measurementss were highly correlated to the litter size (P<0.01), with the equation was  Y= 0.015X1b + 0.011X2b + 0.025X3b - 0.002X4b + 0.022X5b + 0.010X6b + 0.022X7b + 0.030X8b + 0.026X9b.  The chest girth was the most elastic body size measurement to the litter size. The increasing of 1 cm of doe’s chest girth would result in the increase of litter size, each of 0.0545 and 0.0417 heads born-1 at  BPTU-HPT Pelaihari and Cordero farms.  Differences in head size and head shape due to the morphometric adaptations cannot be used to predict litter size.
Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour K. Listyarini; Jakaria Jakaria; M. J. Uddin; C. Sumantri; A. Gunawan
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.549 KB) | DOI: 10.5398/tasj.2018.41.2.100

Abstract

Cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) and kinesin-like protein KIF12 (KIF12) genes are predicted as candidate genes which play important roles in lamb flavour and odour. The aim of this study was to analyse the genotype polymorphism of CYP2A6 and KIF12 genes, to study association and expression of these genes with lamb flavour and odour. Identification of genes polymorphism and associations of CYP2A6 and KIF12 genes were performed using PCR-RFLP method and GLM analysis. The PCR-RFLP products of CYP2A6 and KIF12 were digested by restriction enzyme BsmAI and BfaI, respectively. The expression of CYP2A6 gene was performed using qRT-PCR. The results showed that the CYP2A6 and KIF12 genes were polymorphics. The CYP2A6 gene found to have two genotypes (TT and GT), whereas the KIF12 gene found to have three genotypes (CC, CT, and TT). The CYP2A6 and KIF12 genes were in Hardy Weinberg Equilibrium (HWE). Association analysis showed that CYP2A6 (g.49170107 G>T) was significantly (P<0.05) associated with 3-methylindole (MI) or skatole, while KIF12 (g.9617965 C>T) was not significantly associated with lamb flavour and odour. The GT genotype exhibited a greater 3-methylindole (MI) or skatole than the TT genotype (P<0.05). The mRNA expression analysis showed that CYP2A6 mRNA expression was higher (P<0.01) in animals with the TT genotype. These results will improve the understanding of the functions of the CYP2A6 in lamb flavour and odour, especially in term of 3-methylindole (MI) or skatole compound within the liver and will shed light on CYP2A6 as a candidate in the selection of sheep with low lamb flavour and odour.
Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP D. E. W. T. Sihite; R. Priyanto; Jakaria Jakaria
Tropical Animal Science Journal Vol. 42 No. 3 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.901 KB) | DOI: 10.5398/tasj.2019.42.3.175

Abstract

The aim of this study was to identify the variation of 5’UTR CAPN1 gene and its association to growth traits in Bali cattle. DNA samples were obtained from 80 heads of Bali cattle originated from BPTU-HPT Denpasar. The average of Bali cattle age was 784 days (631 days-1098 days). Bali cattle were divided into 3 age groups namely, the first group (1.5 years to 2 years), the second group (2 years to 2.5 years), and the third group (2.5 years to 3 years). The observed growth traits were birth weight (kg), live weights (kg), average daily gain (kg), body length (cm), chest depth (cm), withers height (cm), hip height (cm), and heart girth (cm). Polymorphism identification of 5’UTR CAPN1 gene was conducted by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with BglII as the restriction enzyme. Growth traits data association were analyzed using the General Linear Model (GLM) analysis. The 5’UTR CAPN1 gene|BglII was polymorphic in Bali cattle (GG, GT, and TT). Genotype frequencies for Bali cattle were 0.30 (GG), 0.66 (GT), and 0.04 (TT). The allele frequencies of G and T allele were 0.63 and 0.37, respectively. The G allele was the most frequent allele and GT genotype was the most frequent genotype among the cattle. The CAPN1|BglII had a significant effect (p<0.05) on growth traits in Bali cattle. Animal carrier of GG genotype had higher live weight and average daily gain than those with GT genotype, while the lowest values were associated with TT genotype.
Genetic Diversity of Indonesian Swamp Buffalo Based on Microsatellite Markers F. Saputra; Jakaria Jakaria; A. Anggraeni; C. Sumantri
Tropical Animal Science Journal Vol. 43 No. 3 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.3.191

Abstract

Indonesia has high genetic resources of local swamp buffalo with good adaptation across regions. However, these animals decline in both population and genetic quality. This research was conducted to study the genetic diversity of Indonesian swamp buffalo. A total of 199 DNA samples (swamp buffalo) from seven provincial populations were used in this study. Genetics identification used three microsatellite markers (CSSM66, ILSTS61, and ILSTS17). Microsatellites were visualized by Polyacrylamide Gel Electrophoresis (PAGE) 10% with silver staining method. Microsatellite data were analyzed using GenAlEx 6.41, Cervus 3.0, and POPTREE2 software. The results showed that a total of 9 alleles were found from the three loci. ILSTS61 had a high PIC (Polymorphism Information Content) compared to the other loci. The high observed heterozygosity of ILSTS61 was found in swamp buffalo from Riau Province, while the Ho value of ILSTS17 ranged from 0.000 to 0.170. This study identified two clusters for Indonesian swamp buffalo, i.e., cluster I (Aceh, North Sumatra, and Riau) and cluster II (Banten, Central Java, West Nusa Tenggara, and South Sulawesi). The two major divergent directions are considered in Indonesia swamp buffaloes across the observed provinces.
Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method R. Azis; Jakaria; A. Anggraeni; A. Gunawan
Tropical Animal Science Journal Vol. 43 No. 4 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.4.306

Abstract

Milk fatty acids are largely affected by genetic factors. Acetyl-CoA Carboxylase Alpha (ACACA) gene is one of the important genes in regulation and metabolic function of milk fatty acids in dairy cattle. The objective of this study was to find out the relationship of single nucleotide polymorphism (SNP) ACACA gene with milk fatty acid trait in local dairy cattle. A total of 277 samples of Holstein Friesian (HF) were collected from Indonesian Research Institute for Animal Production (IRIAP), Animal Breeding Center and Forage Feed of Baturraden (ABCFFB), Central Java Province, Animal Husbandry Training Center of Cikole (AHTCC), West Java Province, Singosari Artificial Insemination Station (Singosari AIS), East Java Province, and Lembang Artificial Insemination (Lembang AIS), West Java Province, Indonesia. Genotyping of this SNP marker (g.2203G>T) was analyzed using the real-time Polymerase Chain Reaction (PCR) based on the hybridization TaqMan probe as the method for allelic discrimination. Milk samples were analyzed using Gas Chromatography and Mass Spectrometry (GCMS). The results of this study revealed the GG and GT genotypes. The proportion of the GG genotype frequency (0.88) was higher than the GT genotype (0.11) and the G Allele frequency was shown higher than the T allele in all locations, i.e., 0.942 and 0.08, respectively. The ACACA gene g.2203G>T SNP was significant (p<0.05) for lauric (C12:0) and dodecanoic (C17:1) acids. It was concluded that the ACACA gene g.2203G>T SNP could be useful as a marker selection for milk fatty acid such as lauric and dodecanoic fatty acids.
Co-Authors . Aryogi . Aryogi, . . Hartati . hartati A Gunawan A Saefuddin A. Alwiyah, A. A. Anggraeni A. Anggraeni A. B. L. Ishak, A. B. L. A. Furqon A. GUNAWAN A. Gunawan A. Gunawan A. Gunawan A. Hubur A.A. Ketut Agung Cahyawan W Acep R Jayaprawira Achmad, Fariz Adefia Fahira Agung, Anak Agung Gde Ahmad Furqon Aina Zahra Parinduri alim, iqbal Amanda, Nadine Amin Wahyudiono Aminurrahman Aminurrahman Aminurrahman Andi Baso Lompengeng Ishak Andriya, Nadya Nurafifah Anggreini, Heny Anis Fauzi Annang Dwijatmiko Asep Gunawan Asep Muhyidin Aulia, Ameytia Rizka Azis, Rosidi B Brahmantiyo B Tappa B. Brahmantiyo Bagus Gaga Partama, Ida Bagus Ramadi Bastanta Ginting, Jeremy Bina, Rasyidin Bonawati, Y. G. M. Bramada Winiar Putra Budiman, C. Burhanuddin C Sumantri C. Budiman C. SUMANTRI C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri Cahyo Budiman Cece Sumantri Chalid Talib Chalid Talib Christabel, Anastasya Devina Cristovao, Adelaide Jose Pereira Cungfinius Finnata Husin D Duryadi D. A. Astuti D. A. Astuti D. Anggrela D. E. W. T. Sihite Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh Dairoh, Dairoh Dairoh, D. Damanik, Nurul Fazira David David Dennisa Amalia Syaka DEWI APRI ASTUTI Dini Hariyanti E Andreas E Sari E. Andreas E. Handiwirawan Edwar Edwar EDWAR EDWAR Eka Haryati Yuliany Eka Putri Budiman, Shelly Eka Ulfiani Elih Sudiapermana, Elih Elizabeth Celine Vega ENDANG TRI MARGAWATI Ervina Mukharomah, Ervina Eryk Andreas F Hasan F Saputra F. Saputra F. Saputra, F. F. W. Pramukti Fahmi Abdillah Fang, Hellen Fang Fauzan Habibi Lubis Fauzi, Rizki Fitra Aji Pamungkas Fitriani Eka Puji Lestari Fuadi Zulkipli G. Ayuningtyas Ghossani, Muhammad Nico Gunadi, Jevan Immanuel Gunawan Sitanggang Gunawan, Cornelia Lisia H Martojo H. Naraini, H. H. Nuraini Hadi S Alikodra Handayani , Handayani Hasmar Husein Nasution Hasrul Bani Asharudin Henny Nuraini Hermansah Hermansah, Hermansah Hermawan, Bambang Hesti Akila Jahra Hikmawaty, Hikmawaty Himmatul Khasanah Holisoh, Iis I I Arief I Khaerunnisa I Komang Gede Wiryawan I Made Londra I. I. Arief I. Inounu I. Inounu I. Khaerunnisa I. Khaerunnisa I. Suhendro Ida Ayu Putu Sri Widnyani Ida Bagus Gaga Partama Iip Saripah, Iip Ilham, Muhammad Ilham Rohman Ima Maisaroh Irma Isnafia Arief Ishak, Andi Baso Lompengeng Isnawati, Endang Istianah Setyaningsih Istiqlal, R. F. Isyana Khaerunnisa Isyana Khaerunnisa Ita Khairani Iwan Ridwan Jessy Mainidar Jollie, Catherine Junitha, Katherine Justine Tanuwijaya K Suzuki K. A. Paramitasari, K. A. K. G. Wiryawan K. Listyarini K. Listyarini K. Listyarini K. Listyarini Kamil, Mustafa Kania Larisa Dwiyani Karimah, S. N. Kevin Alexander, Kevin Khasanah, H. Kholijah Kholijah, K. Kocu, N. Komalasari, Rena Komang Alit Paramitasari Krisnandi, Antonius Rizki Listyarini, K. Londra, I. M. M Kaomini M. A. Abuzahra M. A. Abuzahra M. Baihaqi M. F. Ulum M. J. Uddin M. J. Uddin M. J. Uddin M. Syamsul Arifin Zein M. Yamin M. Yamin M.S.A. Zein Made Londra, I Maesaroh, Iis Mahmud, Rois Mahmudi Mahmudi Maisaroh, Ima Maldini, Dino Manuela, Nancy Manurung, Ruth Geraldine Maria Ulfah Maringka, Tracy Rachel Marwah, Putri Ainun Maryani Maryani Maskur Maskur Masriana Masriana, Masriana Mohamad Yamin Mokhamad Fakhrul Ulum, Mokhamad Mokhmad Fakhrul Ulum Muammar Kadafi Muhammad Afif MUHARRINA HARAHAP, MUHARRINA Muhyidin , Asep Mulyono, R. H. Muslimiah, Muslimiah Musyaddad, T. N. Pratiwi, N. N. Ulupi Najmudin Nanda, Riska Dwi Nashar Utamajaya, Joy Natasha Christania Ananta Nathania Alanta Neyrine, Varlonia Ni Made Paramita Setyani Ni Made Yulia Dewati Ayu, Ni Made Yulia Dewati Ayu Niken Ulupi Noer Ali Tedi Pranata, Moch Nur Faiz Habibah Nuraini Chaniago, Nuraini Nurhayati Nurhayati P. P. Agung, P. P. Padang, Brent Hizkia Pambudi, Bondan Parwanti, Sri Permana, Elih Sudia Pertiwi, E. A. Prastya Idihastuti Prastyono, Bramantio Prisetiadi, Alif Priyambodo, Danang Puji Rianti Putra, I. G. R. Putri Indah Ningtias Putri Yudilestari, Eka Putri, Luh Diva Regina R R Noor R Sari R. Afnan R. H. Mulyono R. Iis Arifiantini R. Ismail R. K. Rusli R. Misrianti R. Mutia R. Mutia R. OKTAVIANI R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Priyanto R. Putri R. R. Noor R. R. Noor R. R. Noor, R. R. R.R. Noor Rachmawati, S. M. Rahma Purba, Abdur Rani Ramdayani Renny Risqiani Restu Misrianti Reza, Muhammad Aulia Riani, Valencia Yosephine Richard Wijaya Putra Ridwan, Habibi Rini Fitriani Rini Herlina Mulyono Ristaviasha, Clarisya Rita Mutia Rizqullah Thohuri Robi Amizar Ronny R Noor Ronny Rachman Noor Rudy Priyanto Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rukmiasih Rulah Assegaf, Sayid Nazir Rusli, Ridho Kurniawan S S Mansjoer S. Darwati S. Elieser S. H. Wijaya S. Hartono S. 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