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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Perikanan dan Kelautan BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL BIOLOGI INDONESIA Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Jurnal Akuakultur Sungai dan Danau Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Buletin Jalanidhitah Sarva Jivitam Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan JURNAL ILMIAH SATYA MINABAHARI

Mia Setiawati

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University, Bogor, West Java, Indonesia

Author-ID : 377250

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Library & Information Science Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary Other

Published : 119 Documents Claim Missing Document

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Utilization of garlic and meniran flour by repelleting method in feed for preventive and curative efficacy of Aeromonas hydrophila in catfish Clarias sp. Wahjuningrum, Dinamella; Kurniawan, Dadang; Setyotomo, Karno; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This research was purposed to determine the effective dose of meniran and garlic flour in feed by repelleting method for preventive and curative efficacy to the infection of Aeromonas hydrophila in catfish. The treatment tested were eight different dosages of garlic and meniran meals (2:1). Those four dosage used to preventive treatment namely A (0.1%), B (1.1%), C (2.1%), and D (3.1%), the other four used to curative treatment namely E (0.2%), F (2.2%), G (4.2%), and H (6.2%). The preventive treatments were given for two weeks before challenging test. The curative treatment was performed on 2nd–8th day after challenging test. Challenging test was carried out by intramuscularly injecting of 0.1 mL A. hydrophila (108cfu/mL) into the fish. Parameters observed were feeding response, growth rate, survival rate, clinical symptoms, wound healing, and organs morphology. The result showed that the survival rate of preventive treatment was 40±40% for A and B, 60±20% for C, and 20±20% for D. The survival rate of curative treatment was 33.33±11.55% for E, 46.67±11.55% for F, 33.33±11.55% for G, and 26.67±11.55% for H. The results indicated that the preventive treatment was more effective than the curative treatment, presumably because the immunostimulatory activities on preventive was better than curative. Keywords: garlic, meniran, repelleting, Aeromonas hydrophila, catfish

The use of Spirulina platensis as tilapiaOreochromis niloticusdiet supplementation Utomo, Nur Bambang Priyo; Rahmatia, Firsty; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Spirulina is blue green algae that have high nutrient content like protein and vitamin so itcan beused as source ofsingle cell protein (SCP). This research was conductedto know minimum doses of Spirulina in different protein feed on growth performance of tilapia. Experimental diets devided by two different protein level (25% of protein and 28% of protein) and three levels supplementation of Spirulina 0% (25A), protein level 25% supplementedSpirulina 3% (25B), protein level 25% supplementedSpirulina 6% (25C), protein level 28% supplementedSpirulina 0% (28A), protein level 28% suplemented Spirulina 3% (28B), and protein level 28% supplementedSpirulina 6% (28C). Testeds feedwere tilapia with average initial weight 17,24±0,29 g and density five fish/aquarium. Tilapia rearedin aquarium sized 50×40×35 cm3 filledwith 30 cm waterand provided altrecirculation system. Fish were cultured for 40 days and fed at satiation with three times feeding frequency. All data analyzed statistically by one-way ANOVA.Result demonstrated that various dosesof Spirulina in different protein level of feed influencedspecific growth rate, feeding efficiency, protein deposition, and fat deposition. Fish were fed by feed containing Spirulina 3% in protein level 25% showed growth performance as same as fed of protein level 28%. Keywords: feed protein, Spirulina, growth performance, tilapia

Dietary vitamin E on the reproductive performance of the fantail goldfish Carassius auratus auratus Arfah, Harton; Melati, .; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This research was conducted to examine the different dose of vitamin E in the diet on female broodstock reproduction performance of the fantail goldfish Carassius auratus auratus. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and was air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56.00±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish Keywords: female broodstock, fantail goldfish, vitamin E, reproduction quality ABSTRAK Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet Carassius auratus auratus. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, gonadosomatic index 8,86±4,62%, fekunditas 56,00±29,18 butir/g ikan, dan germinal vesicle breakdown 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian, suplementasi vitamin E sebesar 375 mg/kg pada pakan adalah dosis terbaik dalam meningkatkan produktivitas induk betina ikan komet. Kata kunci: induk betina, ikan komet, vitamin E, kualitas reproduksi

The growth of red tilapia fed on organic-selenium supplemented diet Suprayudi, Muhammad Agus; Faisal, Burhanudin; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The experiment was conducted to evaluate the level of organic selenium in the diet on the growth performance of red tilapia Oreochromis sp. Four levels of organic selenium namely 0, 1, 2, and 4 g Se/kg diet were used as a treatment, and the selenium content on the diets are 0.12, 0.19, 1.05, and 1.42 mg Se/kg respectively. This experiment were used randomized design with four treatments and two replications. All the diet was formulated to have similar protein and energy. Juvenile red tilapia with average body weight of 9.49±0.94 g were reared in the 80×40×40 cm3 aquarium with density of 10 fish/aquarium. Fish were reared for 40 days and feed four times daily at satiation levels. The result of this study showed that fish fed diet containing 4 g organic Se/kg diet (1,42 mg Se/kg) had the best growth performance. Keywords: organic selenium, growth, red tilapia ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dosis selenium organik pada pakan terhadap kinerja pertumbuhan ikan nila merah Oreochromis sp. Empat macam dosis selenium organik yang digunakan adalah 0, 1, 2, dan 4 g Se/kg pakan. Kandungan selenium pada setiap pakan berturut-turut adalah 0,12; 0,19; 1,05; dan 1,42 mg Se/kg pakan. Penelitian ini menggunakan rancangan acak dengan empat perlakuan dan dua ulangan. Semua pakan diformulasikan memiliki protein dan energi yang sama. Ikan yang digunakan adalah juvenil nila merah dengan bobot rata-rata 9,49±0,95 g yang dipelihara dalam akuarium berukuran 80×40×40 cm3 dengan kepadatan 10 ekor/akuarium. Ikan tersebut dipelihara selama 40 hari dengan frekuensi pemberian pakan empat kali sehari secara at satiation. Hasil penelitian ini menunjukkan bahwa ikan yang diberi pakan yang mengandung 4 g Se organik/kg pakan (1,42 mg Se/kg) memperlihatkan kinerja pertumbuhan terbaik. Kata kunci: selenium organik, pertumbuhan, nila merah

Prevention of Aeromonas hydrophila on catfish juvenile using garlic and shatterstone herb Wahjuningrum, Dinamella; Astrini, Retno; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to determine the dose of garlic and shatterstone herb mixed into the feed in 11-day old catfish juvenile (body length 1.53±0.26 cm, body weight of 40±16 mg). The study was divided into two steps, the first step was dose determination and the second step was the dose testing. The treatment was carried out for 21 days, then the challenge test was conducted by immersion method for 60 minutes in A. hydrophila density of 104 cfu/mL. Parameters of observation were survival, feed intake, relative growth rate, morphology of the liver, and water quality. The result showed that garlic at 25 g/kg and shatterstone herb at 5 g/kg provided higher fish viability (81.11±3.85%) compared with the control (23.00±5.77%). Feed intake and relative growth rate were not different among the treatments. Whereas the liver condition in positive control was paler than other treatments. Thus, prevention of A. hydrophila infection on catfish juvenile was effectively achieved by feeding with diet supplemented by garlic 25 g/kg and shatterstone herb 5 g/kg. Keywords: garlic, shatterstone herb, Aeromonas hydrophila, juvenile, catfish ABSTRAK Penelitian ini bertujuan untuk menentukan dosis bawang putih dan meniran yang dicampur ke pakan dalam bentuk tepung pada benih lele berumur 11 hari (berukuran panjang 1,53±0,26 cm dan bobot 40±16 mg). Penelitian ini terbagi menjadi dua tahap, pertama adalah tahap penentuan dosis (dosis 20 ppt bawang putih+5 ppt meniran dan 25 ppt bawang putih+5 ppt meniran) dan kedua adalah tahap pengujian dosis. Perlakuan diberikan selama 21 hari, kemudian uji tantang dilakukan dengan metode perendaman selama 60 menit dengan kepadatan bakteri A. hydrophila 104 cfu/mL. Parameter yang diamati adalah kelangsungan hidup, jumlah konsumsi pakan, pertumbuhan relatif, morfologi organ hati, dan kualitas air. Hasil penelitian membuktikan bahwa perlakuan bawang putih 25 g/kg dan meniran 5 g/kg memberikan kelangsungan hidup benih lele sebesar 81,11±3,85%, sedangkan kontrol positif 23,00±5,77%. Jumlah konsumsi pakan dan pertumbuhan relatif tidak berbeda antarperlakuan, sedangkan organ hati berwarna pucat pada kontrol positif. Pencegahan infeksi bakteri A. hydrophila dengan pakan campuran bawang putih 25 g/kg dan meniran 5 g/kg efektif dilakukan pada benih ikan lele. Kata kunci: bawang putih, meniran, Aeromonas hydrophila, benih, ikan lele

Pengembangan pemakaian hasil samping agroindustri berbahan dasar jagung sebagai alternatif bahan baku pakan ikan kerapu tikus Cromileptes altivelis Suprayudi, Muhammad Agus; Yaniharto, Dedy; Abidin, Hasan; Priyo Utomo, Nur Bambang; Jusadi, Dedi; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis experiment was conducted to evaluate the use of by product of corn ethanol industry, DDGS (distiller dried grain with soluble) and hominy as feed raw material on digestibility and the growth performance of humpback grouper Cromileptes altivelis juvenile. Three experimental diets containing isoprotein (48%) but different in DDGS contain were used. Diet A containing 0% of DDGS and hominy while diet B and C containing 10.13% (6.13% of DDGS and 4% of hominy) and 19.05% (15.05% of DDGS and 4% of hominy). Reduce of fish meal level in the diet was happen as a consequence of balancing amino acid. For digestibility experiment and Cr2O3 at level of 0.05% was used as a tracer. The feces were daily collected 30‒60 minutes after feeding for 14 days. Dry matter and protein were used as digestibility parameters. Completely randomized design with three treatments and two replicates was used in this experiment. Humpback grouper with an average weight of 11‒14 g were reared at floating net cages (FNG) of 3×2×1.5 m3. The stocking density for each cage was 167 fish/FNG and reared for six months. For digestibility measurement 30 fish were held in cylindrical fiber tank filled with 800 L of sea water. Fish were fed three times daily at satiation level. For maintaining net in a clean condition net was changed in every ten days. Feed consumption, digestibility of protein and total, growth performance indicator i,e specific growth rate, feed efficiency protein and lipid retention were used as evaluating parameters. The results showed that the fish fed with diet containing DDGS (6.13%) dan hominy (4%), at the level of 10.13% (diet B), shows similar growth performance indicators with the fish fed diet A (0% of DDGS and hominy) and the growth performance indicator was higher when being compared to those fish fed diet C (containing 19.05% of DDGS (15.05%) and hominy (4%)) (p<0.05). More over the inclusion of DDGS and hominy at the level of 10,13% give no negatif impact to feed palatability (shown by total feed consumption value) and digestibility of protein. The increasing level of DDGS and hominy more than 10% reduced palatability and protein digestibility. Based on this research, DDGS and hominy can be used up to 10% in the feed formulation of humpback grouperKeywords: digestibility, growth performance, DDGS, hominy, humpback grouper, Cromileptes altivelisABSTRAKPenelitian ini dilakukan untuk mengevaluasi pemanfaatan limbah jagung dari industri etanol dalam bentuk distillate dried grain with soluble (DDGS) dan homini sebagai bahan baku pakan terhadap kinerja pertumbuhan ikan kerapu bebek Cromiliptes altivelis. Ikan yang digunakan dalam penelitian ini adalah juvenil ikan kerapu bebek berukuran 11‒14 g. Ikan dipelihara dalam karamba jaring apung yang berukuran 3×1,5×2 m3 dengan kepadatan 167 ekor/jaring. Ikan diberi pakan sampai kenyang sebanyak dua kali sehari. Untuk menjaga agar jaring tetap bersih maka dilakukan pergantian jaring setiap satu sampai dua minggu sekali. Ikan dipelihara selama lima bulan dan setiap bulan dilakukan sampling. Tiga macam pakan digunakan dalam penelitian ini dengan kadar protein 47% dan rasio energi protein 10 kkal/g protein. Pakan A adalah pakan yang tidak mengandung DDGS dan homini, pakan B mengandung DDGS 6,13% dan 4% (total 10,13%), dan pakan C mengandung DDGS 15,05% dan Homini 4% (total 19,5%). Untuk mengevaluasi ketercernaan nutrien maka kromium oksida (Cr2O3) digunakan sebagai penelusur. Penelitian ketercernaan dilakukan di laboratorium dengan menggunakan fiber dengan volume 800 L. Rancangan acak lengkap dengan tiga perlakuan dan dua ulangan digunakan dalam penelitian ini. Hasil penelitian menunjukkan bahwa nilai ketercernaan nutrien tidak berbeda sampai total kadar DDGS dan homini 10,13% (pakan B) dan peningkatan DDGS serta homini sampai 19,05% (pakan C) menurunkan nilai ketercernaan. Ikan yang diberi pakan dengan DDGS sebesar 10,13% (pakan B) memiliki nilai efisiensi pakan, sintasan, laju pertumbuhan spesifik, konsumsi pakan dan retensi protein yang sama dengan pakan tanpa DDGS (pakan A), akan tetapi nilai-nilai tersebut lebih tinggi jika dibandingkan dengan ikan yang diberi pakan B (p<0,05). Retensi lemak ikan yang diberi pakan B memiliki nilai yang lebih tinggi jika dibandingkan dengan ikan yang diberi pakan A dan C. Dari penelitian ini dapat disimpulkan bahwa DDGS dan homini dapat digunakan sebagai bahan baku pakan ikan kerapu bebek sampai level 10,13% (DDGS 6,13% dan homini 4%).Kata kunci: pertumbuhan, ketercernaan, DDGS, homini, ikan kerapu bebek, Cromileptes altivelis

Survival and growth of catfish Pangasionodon sp. larvae fed on vitamin C-enriched Artemia Setiawati, Mia; Putri, Darina; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The research was conducted to evaluate effect of the administration of Artemia enriched with vitamin C on the survival, total body length, and average body weight of Pangasionodon sp. larvae. Artemia were enriched with either 0, 50, 100, or 150 mg/L vitamin C immersed in the culture medium for 13 hours. Two days old larvae were fed on enriched Artemia 12 times/day for seven days. At the end of feeding experiment, it was found that fish fed on Artemia enriched with 100 mg/L culture medium had the highest survival, total length and body weight, respectively 76.17±12.78%, 1.63±0.34 cm, and 20.6±3.8 mg. Keywords: survival, growth, Artemia, vitamin C, catfish, Pangasionodon sp. ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh Artemia yang diperkaya dengan vitamin C dosis berbeda terhadap kelangsungan hidup, panjang total, dan bobot tubuh larva ikan patin Pangasionodon sp. Pada penelitian ini, Artemia diperkaya dengan vitamin C dosis 0, 50, 100, atau 150 mg/L media pengayaan. Larva yang berumur dua hari diberi pakan Artemia hasil pengayaan sebanyak 12 kali sehari selama tujuh hari masa pemeliharaan. Pada akhir percobaan, didapatkan bahwa larva ikan yang diberi Artemia diperkaya vitamin C dosis 100 mg/L media pengayaan memberikan kelangsungan hidup 76,17±12,78%, panjang total 1,63±0,34 cm, dan bobot tubuh paling tinggi 20,6±3,8 mg. Kata kunci: kelangsungan hidup, pertumbuhan, Artemia, vitamin C, ikan patin, Pangasionodon sp.

Role of various fishmeal ingredients on sangkuriang catfish Clarias sp. growth Utomo, Nur Bambang Priyo; Susan, ,; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This research was aimed to evaluate the role of fish meal from different raw materials in improving growth performance of sangkuriang catfish Clarias sp. The source of raw materials used were derived from trash fish, salted fish, and head fish eal. Fish reared for 44 days and were given feed test three times per day at 08.00, 12.00, and 16.00. Biomass weight of the experimental fish used were 38.49±1.61 g. The fish reared on the aquarium of 60x50x40 cm3 and by using recirculation system. The results showed that fish meal derived from trash fish provide the best result for feed consumption, daily growth rate, absolute length, biomass, retention of protein, retention of fat, feed efficiency, feed conversion (P>0.05). However, it does not significantly different on the survival of fish (P>0.05). The data showed that the use of trash fish meal resulted on the best growth performance of sangkuriang catfish. It was indicated that the trash-fish meal might be used as the main protein source of sangkuriang catfish feed. Keywords: catfish, growth, at satiation, fish meal ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pakan uji yang menggunakan sumber tepung ikan dari bahan baku berbeda dalam meningkatkan kinerja pertumbuhan lele sangkuriang Clarias sp. Sumber tepung ikan yang digunakan berasal dari ikan rucah, ikan asin, dan kepala ikan, yang memiliki umum digunakan sebagai salah satu bahan baku pakan lokal. Ikan diberi pakan at satiation selama 44 hari dengan frekuensi tiga kali sehari, yaitu pada pukul 08.00, 12.00, dan 16.00. Biomassa rata-rata ikan uji yang digunakan adalah 38,49±1,61 g. Ikan dipelihara dalam akuarium berukuran 60x50x40 cm3 dan dengan menerapkan sistem resirkulasi. Hasil penelitian menunjukkan bahwa tepung ikan yang berasal dari ikan rucah mampu memberikan hasil terbaik dalam meningkatkan kinerja pertumbuhan ikan uji. Hal ini ditunjukkan oleh nilai kinerja pertumbuhan seperti jumlah konsumsi pakan, laju pertumbuhan harian, panjang mutlak, biomassa, retensi protein, retensi lemak, efisiensi pemberian pakan, dan konversi pakan yang menunjukkan perbedaan nyata (P<0,05). Namun, tidak terjadi perbedaan pada kelangsungan hidup ikan (P>0,05). Hasil penelitian menunjukkan bahwa penggunaan tepung ikan rucah menghasilkan pertumbuhan terbaik sebagai sumber protein utama pada pakan ikan lele sangkuriang. Kata kunci : ikan lele, pertumbuhan, at satiation, tepung ikan

Digestibility of pre-treated cassava peel as feed ingredient for Nile tilapia Mulyasari, ,; Kurniawati, Feri; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This research aimed at determining digestibility coefficient of cassava peel(Manihot utilissima) after immersion in 3% (w/v) NaOH for three days, fermentation using combined fungi of 10% Trichoderma viride and Phanerochaete chrysosporium for seven days, and fermentation using 15% (w/w) Bacillus megaterium for five days as feed ingredients for Nile tilapia Oreochromis niloticus. Total digestibility test was conducted by mixing 30% of cassava peel and 70% of reference diet. Nile tilapia at the average weight of 16.6 g were used as experimental fish. Fish was held for 28 days in aquarium (50x50x50 cm3) at the density of 10 fish/aquarium. Fish were fed twice daily to satiation. Feces collection started after five days of adaptation to chromium oxide diets. The results showed that the three treatments had significant effects compared to control (P<0.05), protein digestibility of were improved 5%, 15%, and 10%, energy digestibilitiy were 20%, 18%, 16%, and total digestibility of test cassava peel were 174%, 151%, and 164%, respectively. Cassava peel fermented with combined 10% mold showed the highest protein digestibility impliying it potency as feed ingredient for Nile tilapia diet. Keywords: Nile tilapia, cassava peel, NaOH, mold, bacteria, digestibility ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kecernaan kulit ubi kayu (Manihot utilissima) setelah perendaman dengan NaOH 3% (w/v) selama tiga hari, fermentasi kapang Trichoderma viride dan Phanerochaete chrysosporium 10% (w/w) selama tujuh hari, dan fermentasi bakteri Bacillus megaterium 15% (w/w) selama lima hari sebagai bahan baku pakan ikan nila. Uji kecernaan total bahan dilakukan dengan mencampurkan 30% kulit ubi kayu dengan 70% pakan acuan. Ikan uji yang digunakan adalah ikan nila dengan bobot rata-rata 16,6 g. Ikan dipelihara selama 28 hari dengan kepadatan 10 ekor/akuarium berukuran 50x50x50 cm3. Pemberian pakan dilakukan dua kali sehari secara at satiation. Pengumpulan feses dimulai setelah lima hari adaptasi pakan uji yang diberi indikator kromium oksida. Hasil penelitian menunjukkan bahwa ketiga perlakuan memberikan pengaruh yang berbeda nyata terhadap kontrol (P<0,05) dan meningkatkan nilai kecernaan protein pakan berturut-turut sebesar 5%, 15%, dan 10%, nilai kecernaan energi sebesar 20%, 18%, dan 16%, serta nilai kecernaan total bahan sebesar 174%, 151%, dan 164%. Perlakuan kulit ubi kayu yang difermentasi dengan kapang menunjukkan nilai kecernaan protein pakan yang tertinggi sehingga berpotensi sebagai bahan baku pakan ikan nila. Kata kunci: ikan nila, kulit ubi kayu, NaOH, kapang, bakteri, kecernaan

Acute and sublethal toxicity of copper in Siam-catfish juvenile Pangasianodon hypophthalmus Sihono, Dody; Supriyono\, Eddy; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was aimed to determine the level of acute toxicity (LC50) and to analyze the effect of Cu on the survival, growth, haematological, and bioaccumulation of Cu in Pangasianodon hypophthalmus juvenile at sublethal conditions. The experiment trial used completely randomized design with five concentration treatments and two replications to determine LC50. The Cu concentration used were 0, 0.2, 0.7, 1.2, and 1.7 ppm. Sublethal treatment used four Cu concentrations with three replicates. The concentrations were 0, 0.167, 0.334 and 0.667 ppm. Experimental fish used had an average total length 11.0±1.7 cm and weight 13.00±1.72 g. Results showed that Cu was highly toxic to P. hypophthalmus with a LC50-96 hours value of 0.667 ppm (0.539–0.805 ppm). At sublethal concentrations, Cu significantly decreased survival and growth, and increased Cu accumulation started from concentration of 0.167 ppm. Bioaccumulation of Cu started from the highest were in liver, gills, skin and flesh, respectively. Decreased of erythrocytes, haemoglobin and hematocrit indicated anemia, while increased of leukocytes indicated infection and physical stress on the body tissues. Keywords: copper toxicity, haematological, bioaccumulation, Siam-catfish juvenile ABSTRAK Penelitian ini bertujuan untuk menentukan tingkat toksisitas akut (LC50) dan menganalisis pengaruh Cu terhadap sintasan, pertumbuhan, kondisi hematologi, dan akumulasi Cu pada juvenil ikan patin siam (Pangasianodon hypophthalmus) pada kondisi subletal. Rancangan penelitian yang digunakan adalah lima perlakuan konsentrasi dengan dua ulangan untuk menentukan LC50. Konsentrasi Cu yang digunakan adalah 0; 0,2; 0,7; 1,2; dan 1,7 ppm. Pemeliharaan subletal menggunakan empat variasi konsentrasi dengan tiga ulangan. Konsentrasi Cu yang digunakan adalah 0; 0,167; 0,334; dan 0,667 ppm. Ikan uji yang digunakan memiliki panjang total rata-rata 11,0±1,7 cm dan berat 13,00±1,72 g. Hasil menunjukkan bahwa Cu sangat toksik terhadap juvenil P. hypophthalmus dengan nilai LC50-96 jam sebesar 0,667 ppm (0,539–0,805 ppm). Pada konsentrasi subletal, Cu berpengaruh nyata terhadap penurunan sintasan dan pertumbuhan serta menyebabkan peningkatan akumulasi Cu mulai pada konsentrasi 0,167 ppm. Bioakumulasi Cu secara berurutan mulai dari yang tertinggi yaitu pada hati, insang, kulit dan daging. Penurunan eritrosit, hemoglobin dan hematokrit menunjukkan terjadinya anemia, sementara peningkatan jumlah leukosit menunjukkan infeksi dan stres fisik pada jaringan tubuh. Kata kunci: toksisitas tembaga, hematologi, bioakumulasi, bioakumulasi, sintasan, juvenil patin siam

Co-Authors , Alimuddin , Mulyasari , Susan . Kurdianto . Melati . Yuniarti A.D. Akbar Ade Dwi Sasanti Afiff , Usamah Agus Oman Sudrajat Alimuddin Alimuddin A alimuddin alimuddin Aliyah Sakinah, Aliyah Amelia Oktaviani, Amelia Apriana Vinasyiam Arini Resti Fauzi Aris Tri Wahyudi Arlita, Kriswidya Artin Indrayati Asda Laining Asda Laining Atma Jaya Salman Muin Azmi Afriansyah Bambang Priyo Utomo Bianingrum Bianingrum Burhanudin Faisal, Burhanudin C. Nuraeni D. Jusadi D. Shafruddin Dadang Kurniawan Dadang Syafruddin Dairun, Suclyadi Darina Putri Darsiani Darsiani Darsiani Darsiani DEDI JUSADI Dedi Jusadi Dedi Jusadi dedy yaniharto Dewi Yuniati Dewi Yuniati Diamahesa, Wastu Ayu Dian Hardiantho Dian Hardianto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dody Sihono Dwinda Pangentasari Eddy Supriyono Eka Hidayatus Solikhah Eko Harianto, Eko Enang Harris Erni Susanti Fahmi Hasan, Fahmi Fahmi Rajab Fardila Putri, Rizqiyatul Fauzan, Agung Luthfi Fauzi, Arini Resti Febrina Rolin Ferdinand Hukama Taqwa Feri Kurniawati Firsty Rahmatia Gamel Koncara Goro Yoshizaki Grenda Audia Wuryas Pradita Negara Hany Handajani Harton Arfah Hasan Abidin Hendriana, Andri I Mokoginta I Nyoman Adi Asmara Giri I. Mokoginta I. Tepu Ichsan Achmad Fauzi Iis Diatin Ika Wahyuni Putri Imlani, Ainulyakin Hasan Imron Imron, Imron Inem Ode Ing Mokoginta Iqbal Kurniawinata, Mohamad Irzal Effendi Iskandar, Andri Ismail Rahmat Ismarica, Ismarica Istifarini, Mita Ita Apriani Jefry Jefry Jufri, Fatahillah Maulana Juli Ekasari Julia Eka Astarini Julie Ekasari Kaliky, Nunun Ainun Putri Sari Banun Karno Setyotomo Ketut Sugama Ketut Sugama Khasanah, Noviati Rohmatul Kukuh Nirmala Kusriyati Kusriyati L. Indriastuti La Muhamad, Idul Laifa Farihah M. Zairin Junior M.A. Suprayudi MA Suprayudi Mala Nurilmala Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mohammad Aghistni Rahman Mohammad Mukhlis Kamal Muhamad Yamin MUHAMMAD AGUS SUPRAYUDI Muhammad Safir Muhammad Zairin Jr. Muhammad Zairin Jr. MUNTI YUHANA N. Nurjanah N.B.P Utomo N.R. Azwar Nadisa Theresia Putri Naufal, Muhammad Restya nFN Safratilofa Niagara, Niagara Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyoutomo Nur Hikma Mahasu Nur, Abidin Nuraini Annisa, Nuraini Nurhayati Nurhayati Nuri Kamaliah, Syarifah Nurly Faridah Odang Carman Ode, Inem P. Purnama Pangentasari, Dwinda Pattipeilohy, Christian Ernsz Putri Pratamaningrum Arifin Putri Utami, Putri R. Affandi Rahma Dini Arbajayanti Rahma Dini Arbajayanti Rahma, Balqis Aulia Rahmadani Rahmadani Rakhmawati, Rakhmawati, Rasidi Rasidi Rasidi Rasidi Retno Astrini Reza Samsudin RIDWAN AFFANDI Riska Diana Rizkan Fahmi Ronny I. Wahju Shella Marlinda Sheny Permatasari Shidik, Taufik Shidik Adi Nugroho SITI KHODIJAH Siti Khodijah Siti Murniasih Sri Nuryati Sri Nuryati Suardi Laheng Suci antoro Suclyadi Dairun Sujono Sujono Sukenda, Sukenda, Sumantri, Iwan Sumiana, I Kadek Suryadi Saputra Syefti Palmi, Revita T.M. Haja Almuqaramah Talita Shofa Adestia Tatag Budiardi Thoy Batun Citra Rahmadani TI Winarno Toshiro Masumoto Triana Retno Palupi Upmal Deswira Uttari Dewi W Manalu W. Manalu Wahyu Pamungkas Wahyudi, Imam Tri WAODE MUNAENI Wasjan WIDANARNI WIDANARNI Widya Puspitasari Wildan Nurussalam Windu Sukenda Wiwik Hildayanti Wiyoto Wiyoto Y. Hadiroseyani Yonvitner - Yuli Andriani Yuni Puji Hastuti

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