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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Sain Veteriner Jurnal Ilmu Pertanian Indonesian Journal of Electronics and Instrumentation Systems IJCCS (Indonesian Journal of Computing and Cybernetics Systems) Jurnal Pangan dan Gizi Dinamik Media Informatika Seminar Nasional Aplikasi Teknologi Informasi (SNATI) Jurnal Bahtera - Jurnal Pendidikan Bahasa dan Sastra Indonesia Jurnal Ilmu Komputer Jurnal Veteriner Jurnal Sosial Ekonomi Pesisir Jurnal EMPATI Journal of Tropical Soils Cropsaver : Journal of Plant Protection Otopro Sains Tanah JUTI: Jurnal Ilmiah Teknologi Informasi Explore: Jurnal Sistem Informasi dan Telematika (Telekomunikasi, Multimedia dan Informatika) Jurnal Penelitian Pascapanen Pertanian Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Pro Bisnis Indonesian Journal of Biotechnology Jurnal Varidika Biopropal Industri Jurnal Didascein Bahasa JITEK (Jurnal Ilmiah Teknosains) BIOEDUSCIENCE JURNAL BAHASA, SASTRA DAN PEMBELAJARANNYA Jurnal Kimia Terapan Indonesia Pendas : Jurnah Ilmiah Pendidikan Dasar JURNAL TEKNIK INFORMATIKA DAN SISTEM INFORMASI ADMINISTRASI PUBLIK AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Pembahsi (Pembelajaran Bahasa Dan Sastra Indonesia) Jurnal ACSY : Jurnal Accounting Politeknik Sekayu JURNAL PENDIDIKAN TAMBUSAI Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) IJID (International Journal on Informatics for Development) Andragogi: Jurnal Diklat Teknis Pendidikan dan Keagamaan Jurnal Cendikia jurnal Ilmu Pertanian (Agricultural Science) Jurnal Ilmiah Inovasi Menara Ilmu Golden Age: Jurnal Ilmiah Tumbuh Kembang Anak Usia Dini Social, Humanities, and Educational Studies (SHEs): Conference Series Al-Insyirah Midwifery : Jurnal Ilmu Kebidanan (Journal of Midwifery Sciences) Jurnal Teknik Mesin Cakram Jurnal Equation: Teori dan Penelitian Pendidikan Matematika Cendekiawan PRIMA: Journal of Community Empowering and Services Jurnal Ilmu Pemerintahan Suara Khatulistiwa Jurnal EK dan BI Planta Simbiosa : Jurnal Tanaman Pangan dan Hortikultura JURNAL ILMIAH AGRINECA Edudikara: Jurnal Pendidikan dan Pembelajaran Al-Ulum: Jurnal Pendidikan Islam PrimEarly : Jurnal Kajian Pendidikan Dasar dan Anak Usia Dini (Journal of Primary and Early Childhood Education Studies Jurnal Minda Teknik: Jurnal Ilmu Teknik dan Informatika An-Nahdlah: Jurnal Pendidikan Islam Majalah Ilmiah Manajemen dan Bisnis Kresna: Jurnal Riset dan Pengabdian Masyarakat Jurnal Kolaboratif Sains Jurnal Media Akuntansi (MEDIASI) SRIWIJAYA JOURNAL OF ENVIRONMENT Idarah Tarbawiyah: Journal of Management in Islamic Education Al-Ulum: Jurnal Pendidikan Islam Lentera Perawat Edu Society: Jurnal Pendidikan, Ilmu Sosial dan Pengabdian Kepada Masyarakat Jurnal Riset sosial humaniora, dan Pendidikan (Soshumdik) NUSANTARA: Jurnal Pengabdian Kepada Masyarakat Jurnal Manajerial Bisnis Diadik: Jurnal Ilmiah Teknologi Pendidikan Jurnal Psikologi Universitas Gadjah Mada Jurnal Pengabdian Masyarakat Waradin Jurnal Manajemen Bisnis Kewirausahaan International Journal of Educational Research Excellence (IJERE) Dewantara: Jurnal Pendidikan Sosial Humaniora Jurnal Pengabdian Kepada Masyarakat Jurnal Sistem Informasi Mahakarya (JSIM) Gunung Djati Conference Series Jurnal Kedokteran Hewan Jurnal Bintang Pendidikan Indonesia Civitas Consecratio: Journal of Community Service and Empowerment Jurnal Riset Manajemen dan Ekonomi Gudang Jurnal Multidisiplin Ilmu Jurnal Kampus STIKes YPIB Majalengka GEMBIRA (Pengabdian Kepada Masyarakat) AmaNU: Jurnal Manajemen dan Ekonomi Ardhi: Jurnal Pengabdian dalam Negri Oshada Jurnal Ilmiah Kesehatan Pernus Ulumul Syar’i : Jurnal Ilmu-Ilmu Hukum dan Syariah JABI: Jurnal Abdimas Bhakti Indonesia Jurnal Pengabdian Mitra Persada PESHUM Journal for Quality in Women's Health Early Childhood Islamic Education Journal Artificial Intelligence and Computing
Sri Hartati
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Journal : Jurnal Veteriner

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Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.315 KB)

Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.
Kejadian Leptospirosis pada Anjing di Daerah Istimewa Yogyakarta (CASE OF CANINE LEPTOSPIROSIS IN THE CITY OF YOGYAKARTA) Guntari Titik Mulyani; Sri Hartati; Yuli Santoso; Kurnia Kurnia; Agung Budi Pramono; Dyah Kunti Wirapratiwi
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.099 KB) | DOI: 10.19087/jveteriner.2017.18.3.403

Abstract

Leptospirosis is a zoonotic disease, which is caused by Leptospira interrogans. The incidence of leptospirosis in dogs varies according to region and season, and is considered as emerging infectious diseases in humans. Clinical symptoms of leptospirosis in dogs vary greatly, some dogs are asymptomatic, with mild symptoms, and others progress to severe illness until death. The study aims to determine cases of canine leptospirosis in Yogyakarta, and identify the serovar that infect them. A total of 20 dogs without symptoms, with mild symptoms, to severe symptoms of leptospirosis were collected their serum and further tested using Microscopic Agglutination Test (MAT) conducted at the Center for Veterinary Research (BBLitvet) Bogor. History of vaccination is recorded as a consideration in interpreting the MAT results. The results showed that three out of the 20 samples were positive leptospirosis. Of the three positive samples, one was identified as serovar bataviae, another one as serovar bataviae and tarrasovi, and the last as serovar bataviae, tarrasovi, ichterohaemorrhagiae, canicola, celledoni, pyrogenes, cynopteri, and rachmati, respectively. The three dogs with leptospirosis showed similar clinical symptoms i.e. Anorexia, lethargy, and fever. It can be concluded that there are cases of canine leptospirosis in Yogyakarta which is predominantly caused by Leptospira interrogans serovar bataviae. ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh Leptospira interrogans. Kejadian leptospirosis pada anjing bervariasi menurut wilayah dan musim, dan dianggap sebagai penyakit menular yang muncul pada manusia. Gejala klinis leptospirosis pada anjing sangat bervariasi, beberapa anjing tanpa menunjukkan gejala, dengan gejala ringan, dan yang lain melanjut menjadi penyakit yang parah sampai kematian. Penelitian ini bertujuan untuk mengetahui kasus leptospirosis pada anjing di Propinsi Daerah Istimewa Yogyakarta, dan mengidentifikasi serovar yang menginfeksinya. Sebanyak 20 ekor anjing tanpa gejala, dengan gejala ringan, sampai gejala parah leptospirosis diambil serumnya guna pemeriksaan Microscopic Agglutination Test (MAT) yang dilaksanakan di Balai Besar Penelitian Veteriner (BBLitvet), Bogor. Sejarah vaksinasi dicatat sebagai bahan pertimbangan dalam menginterpretasi hasil pemeriksaan MAT. Hasil penelitian menunjukkan bahwa tiga dari 20 sampel positif leptospirosis. Satu sampel positif terhadap serovar Bataviae, satu sampel positif terhadap serovar Bataviae dan Tarrasovi, dan satu sampel lagi positif terhadap serovar Bataviae, Tarrasovi, Ichterohaemorrhagiae, Canicola, Celledoni, Pyrogenes, Cynopteri, dan Rachmati. Anjing yang positif leptospirosis menunjukkan gejala klinis yang sama berupa anoreksia, kelemahan, dan demam. Dari hasil penelitian ini dapat disimpulkan bahwa terdapat kasus leptospirosis pada anjing di Yogyakarta yang penyebabnya dominan oleh Leptospira interrogans serovar Bataviae.
Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton Asmarani Kusumawati; Atik Ratnawati; Ida Arlita Wulandari; Sri Hartati; Tri Untari
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.127 KB)

Abstract

Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.11

Abstract

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.
Co-Authors A. Intan Niken Tari Abdiansah, Abdiansah Abdurrakhman WKA Ade Andriani Adi Prasetia Nanda Afifah Khairiyyah Afrida, Dede Agung Budi Pramono Agus Budiyono Agus Harjoko Agus Wartapa, Agus Agustina Intan Niken Tari Ahlijati Nuraminah Aisyah Citra Febrianti Akhmad Vikry AKTAVIA HERAWATI Al Jabbar, Brama Aldri Frinaldi Alfarisa Nururrozi Amin Budiastuti Aminuyati Ana Maria Gadi Djou Andriyani . Angelina Anastasha Annisa Nurilahi Aridhanyati Arifin Arif Nursetyo Aris Budianto Arjoni Arjoni Asmarani Kusumawati Asmarani Kusumawati Asmoro, Novian Wely Asrianty Mas’ud ati sulianty Atik Ratnawati Ayu Meilani Ayu, Yusmaini Azhari Azhari Azhari Subanar Bambang Sugiantoro Bambang Sumarwoto Bernard Renaldy Suteja Budi Hariono Catur Gunawan Podo Winarno Chiekal Mulia Danar Dono Daniel Happy Putra Danur Azissah Daulat Saragi Dedik Budianta DEFI WULANDARI Dewanto Harjunowibowo Dewi Susanti Dhinny Novryanthi Dwi Priyo Ariyanto Dwi Priyowidodo Dwi Susilo Utami Dyah Kunti Wirapratiwi Edduar Hendri, Edduar Eilen Ardi Estiningtyas El Thaariq Is'ad Elen Erniasita Elisabet Yunaeti Anggraeni Elyandari, Wiwik Septerian Ema Utami Emma Lilianti, Emma Endah Yulia Endang S Rahayu Endro Yunianto Eni Harmayani Enny Sudarmonowati Enny Sudarmonowati Eri Zuliarso Ernawati Hamidah Ersam Mahendrawan Fanessa Febryan Ruswandi Fauzy Rachman Finta Amalinda Fitri Nurbaiti Fitria Meilina Fitria Roviqowati Guntari Titik Mulyani Guntoro Guntoro Guntoro Guntoro Hadiman Hadiman Handoyo, Alfandy Warih Hani Fitriani Hargiani, Fransisca Xaveria Haries Anom Susetyo Aji Nugroho Hary Purnamaningsih Hastari Wuryastuti Hence Beedwel Lumentut Hendro Pramono, Doni Eko Heni Pujiastuti Heri Budianto Herlina Lidiyawati Hermansyah Hermansyah Herry Cahyana Hery Widijanto Hidayani Syam I Usuman Ida Arlita Wulandari Ida Damayanti Ida Fitriana Ida Suryani Idarianty Irkham Widiyono Irma Julita Izzati J.Sri Widada Jati Sasongko Wibowo Jauhari Syamsiah Jauhari Syamsiyah Jauhari Syamsiyah Jazi Eko Istiyanto Joannes Sri Widada Joko Prastowo Julius Hammer jurnalpps pps Jusuf Luturmas Kamesyworo Kamiso H.N. Kanaya Yose Putri Karolus Charlaes Bego Komariah Komariah Krisdyanto Ariwibowo Kurnia Kurnia Kusai Kusrini Kusrini Kuswarini Kuwat Triyana Laksono Trisnantoro Lela aini Leonardus B.S.Kardono lia saputri Linayanti Linayanti Luciana Djaya Luh Putu Vera Astri Luqyana Dhiya Ulhaq Lutiyah, Lutiyah M Fatoni Kurnianto M Islam Mahdi M. Arif M.Isnando Tamrin MA Barokatul Qodiri Sidomulyo Made Slamet Mahardika, Klara Vamiga Permata Mahasir, Mahasir mar'atus solikha Masrukhan Masrukhan Masythah, Irma Maulan Irwadi Maya Zefriani Lubis Meci Yuniastuti Rahma Meity Suradji Sinaga Mestika Pudan Purba Meylina Diah Ekasari Mia Sukma Dewi Mitri Febyana Moh. Arief Bonis S Mohamad Zaki Taufik Muhammad Alfin Vajriansyah Muhammad Haikal Fuady Muhammad Hanafiah Muhammad lhsan Sarita Muhammad Rifaldi Mujiyo Mujiyo Mukhamad Agung Yusuf Mulud Suhardjo Mustar Musthofa Musthofa Mutiara Rachmawati Suseno Mutiasari Mutiasari Nadhrota Na’imi Nurul Hayati Nafratilova Septiana Nanang Taryana Nanda Rizki Wildani Nanik Rahmani Nanik Rahmani Natasya Wahyu Ningsih Nawawi Nawawi nFN Yopi Niken Anggreani Nisa Sholehah Pangsuma Nita Ashari Noor Istifadah Nurul Hasanah Nurul Ihsani Nurwijayanti Oga Sandria Ongko Cahyono Partomuan Simanjuntak Pebrin Gultom Pipin Sri Sudewi Pitrawana Pitrawana Prasetyo, Indra Pratiwi Sapani Tanjung Purwanto Purwanto Putri Fauziah Putri Nilam Sari Reh Bungana Br Perangin-Angin Retantyo Wardoyo Retni Rifka Dhiya Ulfa Rika Meliansyah Rindia Septa Kurnia Rio Adi Pradana Rio Kurniawan Romaito Gultom Rosalina Anindia Sari Kartika Rosalina Anindya Kartika Rosalina Anindya Sari Kartika RR. Ella Evrita Hestiandari Rugayah Rugayah Rury Rizhardi Rusdi Efendi Sabrina Salamudin Sarah Azzukhruf Sari, Venta 'Yulia Setiawan, Mukhammad Andri Shinta Maharani Shofwatul ‘Uyun Sibarani, Asni Silvia Adimiharta SITI FATIMAH Siti Helmyati Siti Isnijah S.P. Siti Kurniawati Siti Mayang Sari Siti Nurhalisah Siti Safitri Siti Um-um Andriyani Bahroen Slamet Minardi Sonhaji Sonhaji *), Ferra Ika Wardani Solechan Sri Gustina Lubis SRI HENDRASTUTI HIDAYAT SRI KURNIA DEWI Sri Kusumadewi SRI LESTARI Sri Nurdiati Sri Pujiyati Sri Wahyuni Sri Widada Sri Yunita subanar subanar Sumani Sumani Sumarno Sumarno Sumartono . Supriyadi Supriyadi Suryo Wiyono Susanti Budiastuti SUSENO SUSENO SUYONO Suyono . Syawaluddin Syawaluddin Taufik Taufik Teuku Afzal Livandi tisrin maulina dewi Titien Ng Praptosuwiryo Tri Susilowati Tri Untari Tri Widayatsih Triana, Heny Triyanto Triyanto Tuti Rohani, Tuti Tyas Utami Ukit Ulumuddin Ulumuddin Vera Marettianada Widayanti Widia Tirta Sari Wijo Priyono Wisnu Nurcahyo Woro Wulandari WULANDARI Yakobus Ndona Yani Cahyani Yanwar Habibul Muttaqin Yasminingrum Yeni Afrida Yeni Agus Nurhuda Yohan Yohan Yova Atika Yuli Santoso Yunaeti Anggraeni, Elisabet Yusria Zulkarnain Zulkarnain