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Journal : Grimsa Journal of Science Engineering and Technology

Evaluation of Antibacterial Properties from Endophytic Fungi of Chrysanthemum indicum (L.) Flowers against Escherichia coli and Staphylococcus aureus Titah Amelia Ratte; Fatimawali Fatimawali; Trina Ekawati Tallei; Elly Juliana Suoth; Irma Antasionasti; Paulina Yamlean
Grimsa Journal of Science Engineering and Technology Vol. 1 No. 2 (2023): December 2023
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v1i2.15

Abstract

Uncovering the therapeutic potential of secondary metabolites produced by plants, animals, and microbes constitutes the foundation for the development of novel medications. The objective of this investigation is to discern the classes of secondary metabolites and assess the antibacterial properties of endophytic fungal extracts obtained from Chrysanthemum indicum L. flowers. Through the isolation process, five isolates designated as JEC1, JEC2, JEC3, JEC4, and JEC5 were identified. The cultivation of endophytic fungal isolates spanned a three-week period before undergoing extraction with ethyl acetate. The phytochemical tests revealed the presence of alkaloids, flavonoids, steroids, terpenoids, saponins, and tannins in the ethyl acetate extract. Antibacterial activity was determined using the agar well diffusion method, with ciprofloxacin serving as a positive control. Notably, all ethyl acetate extracts from endophytic fungi exhibited antibacterial activity. The most substantial inhibitory diameter against Staphylococcus aureus was recorded as 19.1±0.8 mm for the JEC3 endophytic fungi, while Escherichia coli exhibited an inhibitory diameter of 16±1.1 mm for the JEC2 endophytic fungi.
Evaluation of Antibacterial Properties from Endophytic Fungi of Chrysanthemum indicum (L.) Flowers against Escherichia coli and Staphylococcus aureus Ratte, Titah Amelia; Fatimawali, Fatimawali; Tallei, Trina Ekawati; Suoth, Elly Juliana; Antasionasti, Irma; Yamlean, Paulina
Grimsa Journal of Science Engineering and Technology Vol. 1 No. 2 (2023): December 2023
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v1i2.15

Abstract

Uncovering the therapeutic potential of secondary metabolites produced by plants, animals, and microbes constitutes the foundation for the development of novel medications. The objective of this investigation is to discern the classes of secondary metabolites and assess the antibacterial properties of endophytic fungal extracts obtained from Chrysanthemum indicum L. flowers. Through the isolation process, five isolates designated as JEC1, JEC2, JEC3, JEC4, and JEC5 were identified. The cultivation of endophytic fungal isolates spanned a three-week period before undergoing extraction with ethyl acetate. The phytochemical tests revealed the presence of alkaloids, flavonoids, steroids, terpenoids, saponins, and tannins in the ethyl acetate extract. Antibacterial activity was determined using the agar well diffusion method, with ciprofloxacin serving as a positive control. Notably, all ethyl acetate extracts from endophytic fungi exhibited antibacterial activity. The most substantial inhibitory diameter against Staphylococcus aureus was recorded as 19.1±0.8 mm for the JEC3 endophytic fungi, while Escherichia coli exhibited an inhibitory diameter of 16±1.1 mm for the JEC2 endophytic fungi.
From Nature to Laboratory: The Impact of Leilem Leaves’ Ethanol Extract on Pancreatic Lipase Enzyme Activity Siringo-Ringo, Aurian Fricilia; Fatimawali, Fatimawali; Bodhi, Widdhi; Manampiring, Aaltje E.; Kepel, Billy J.; Budiarso, Fone D.H.
Grimsa Journal of Science Engineering and Technology Vol. 2 No. 1 (2024): April 2024
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v2i1.23

Abstract

For many years, there have been theories on the possibility of preventing or delaying type 2 Diabetes Mellitus by altering some of its risk factors. Drugs that combat obesity have recently been researched concerning the prevention of type 2 Diabetes Mellitus. Inhibition of the digestive enzyme pancreatic lipase is a potential therapeutic strategy in treating and managing chronic diseases such as diabetes and obesity. Plants containing bioactive compounds are identified as potential sources of pancreatic lipase enzyme inhibitors. The use of natural compounds in inhibiting pancreatic lipase enzyme activity is considered to have more potential due to low toxicity and side effects. This study aims to determine the potential and activity effect of ethanol extract of leilem leaves on inhibiting pancreatic lipase enzyme. This study is a laboratory experimental study, the method of measuring lipase inhibition potential was performed using porcine lipase and PNPB with several modifications and using Orlistat as a positive control. Readings were taken using an ELISA reader at a wavelength of 405 nm. The data were then processed to obtain the IC50 value and relative potency. The results of in vitro studies have shown the potential of leilem leaf extract to inhibit pancreatic lipase enzyme activity. Qualitatively, the results showed that leilem leaf extract contains secondary metabolite compounds such as Alkaloids, Flavonoids, Tannins, Saponins, Steroids, and Terpenoids. Quantitatively, the results showed that the ethanol extract of leilem leaves had an absorbance value at the lowest concentration of 1.346 ± 0.53 and 0.709 ± 0.29 for the highest concentration. The IC50 result obtained was 137.89 μg/mL while the IC50 value of the positive control Orlistat was 77.022 μg/mL. Ethanol extract of leilem leaves (Clerodendrum minahassae Teijsm. & Binn) has a potential value of 0.558.
Efficacy of Purple Chrysanthemum Ethanol Extract in Inhibiting Staphylococcus aureus and Escherichia coli Growth Paukiran, Degol; Budiarso, Fona; Manampiring, Aaltje; Fatimawali, Fatimawali; Kepel, Billy Johnson; Bodhi , Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.63

Abstract

Staphylococcus aureus, a Gram-positive bacterium, and Escherichia coli, a Gram-negative bacterium, are known to cause both enteric and systemic infections in humans. The improper use of antibiotics has often been associated with the emergence of antibiotic resistance. Purple chrysanthemum (Chrysanthemum morifolium) has shown significant potential as an alternative treatment, owing to its strong antioxidant, anti-inflammatory, and antibacterial properties. These properties are attributed to the presence of bioactive compounds such as polyphenols, flavones, essential oils, and other phytochemicals. This study aimed to identify secondary metabolites and evaluate the antibacterial activity of ethanol extracts from purple chrysanthemum (Chrysanthemum morifolium). The extraction process was conducted using Ultrasonic-Assisted Extraction (UAE), and the antibacterial activity was assessed using the disc diffusion method at extract concentrations of 12.5%, 25%, and 50%, with ciprofloxacin serving as the positive control. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, phenolics, and steroids in the ethanol extract of purple chrysanthemum. Importantly, all ethanol extracts demonstrated antibacterial activity. The highest inhibition zones were observed at a 50% concentration, with S. aureus showing an inhibition zone of 12.35 ± 0.4 mm, while E. coli exhibited an inhibition zone of 15.08 ± 0.22 mm.
In Vitro Analysis of Anti-Inflammatory Effects of Purple Chrysanthemum (Chrysanthemum morifolium) Leaf Extract Nastiti, Gemi; Fatimawali, Fatimawali; Budiarso, Fona Dwiana Hermina; Manampiring, Aaltje Ellen; Kepel, Billy Johnson; Bodhi, Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.70

Abstract

Chronic inflammatory diseases are the most significant cause of death in the world and pose the greatest threat to human health. Treatment of inflammation is usually done by taking anti-inflammatory drugs that have dangerous side effects in long-term use. Chrysanthemum is the largest horticultural commodity in North Sulawesi that also holds great potential in the world of health. Based on its phytochemical content, purple chrysanthemum leaves have potential as an anti-inflammatory agent. This study aimed to analyze the anti-inflammatory activity of ethanol extract of purple chrysanthemum leaves using the red blood cell membrane stabilization method. This study used an in vitro laboratory experimental method using rat blood. The anti-inflammatory activity of the extract was tested using the red blood cell membrane stabilization method at 4 concentration series, namely 50 ppm, 100 ppm, 200 ppm, and 400 ppm, then measured on a UV-Vis spectrophotometer. The results of phytochemical screening showed that the ethanol extract of purple chrysanthemum (Chrysanthemum morifolium) leaves contains active compounds such as flavonoids, alkaloids, tannins, steroids, and phenolics. The ethanol extract of purple chrysanthemum leaves exhibits anti-inflammatory activity, with higher extract concentrations demonstrating stronger anti-inflammatory effects. The stability percentage values obtained at 50, 100, 200, and 400 ppm were 28.87%, 41.85%, 47.41%, and 64.15% with an IC50 of 235.88 ppm and a correlation coefficient of 0.94.
Mechanistic Exploration of Moringa oleifera Leaf Bioactives in Prostate Cancer Using Network Pharmacology Tengke, Feniea Wulan Agaype; Tallei, Trina Ekawati; Turalaki, Grace Lendawati Amelia; Tendean, Lydia Estelina Naomi; Kaseke, Martha Marie; Niode, Nurdjannah Jane; Fatimawali, Fatimawali
Grimsa Journal of Science Engineering and Technology Vol. 4 No. 1 (2026): April 2026
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v4i1.105

Abstract

Prostate cancer remains one of the most prevalent malignancies in men worldwide, while current therapeutic strategies are often associated with adverse effects and the emergence of drug resistance, highlighting the need for safer and more effective alternatives. This study aimed to elucidate the multitarget molecular mechanisms of bioactive compounds derived from Moringa oleifera leaves against prostate cancer using an integrated in silico network pharmacology approach. A total of 137 bioactive compounds were collected from LC–MS/MS profiling data and phytochemical databases, of which 20 compounds with high predicted biological activity were prioritized based on Way2Drug PASS analysis. Potential protein targets were predicted and intersected with prostate cancer–associated genes to identify shared targets. Protein–protein interaction network analysis revealed 536 overlapping targets, with ten hub proteins, ESR1, AKT1, SRC, EGFR, TP53, HSP90AA1, PIK3CA, HSP90AB1, PIK3R1, and MAPK1, identified as central nodes. Pathway enrichment analysis demonstrated that these targets were predominantly involved in cancer-related signaling pathways, including pathways associated with EGFR tyrosine kinase inhibitor resistance. Pharmacokinetic and toxicity assessments indicated that several compounds, such as isorhamnetin, kaempferol, tocopherol, and afzelin, exhibited favorable drug-likeness properties and low predicted toxicity. Overall, these findings suggest that bioactive compounds from Moringa oleifera leaves exert anticancer effects through a multitarget and multi pathway mode of action rather than single-protein modulation. This study provides systematic insight into the molecular mechanisms underlying the anti–prostate cancer potential of Moringa oleifera leaves and supports their relevance as candidates for further experimental validation and drug development.
Co-Authors Aaltje E. Manampiring Aaltje E. Manampiring Aaltje E. Manampiring Aaltje Ellen Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Abas, Abdul Hawil Abdul Jalil Abubakar, Poetry M.S. Abubakar, Poetry Melinda Adeanne Wullur Adikila, Gregorius Giani Adithya Yudistira Adrian Umboh Ahmad Haekal Alamri, Firdaus Alexander S. L. Bolang Andini Andrew Pangemanan, Andrew Angelique Baya, Gloria Prisca Susana Angelique C. M. Tanan Anggelia Nelisa Kapantow Angle Sorisi Anita Linda Yohana Pratasik Anita Mamuaya Anita Rahel Meilina Paendong Anjely Jessica Makatempuge Annisa Hamka Antasionasti, Irma Apolonia Amelintje Gusmão Awoah Asriyana S Kaseng Astrid Noviera Iksan Axl L. Windah Ayu Fauzia Malinda B H. R. Kairupan Baharuddin, Fatmawaty Bahter, Julia V.F. Barasarathi , Jayanthi Basalamah, Rizqah BEIVY JONATHAN KOLONDAM Bernadus, Zefanya Bernadus, Zefanya G Billy J Kepel Billy J Kepel Billy J. Kepel Billy J. Kepel Billy Johnson Kepe Billy Kepel Billy Kepel Billy Kepel Bodhi , Widdhi Budiarso, Fone D.H. Budiarso, Hermina Butue, Leobernard Catur Riany Celik, Ismail Changiz Mohiyeddini Cheisy Anastasya Gratia Lengkong Chintya G. Derek Damaris, Damaris Datu, Olvie S. Datu, Olvie Syenni Debbie Retnoningrum Debora Tandi DEBY AFRIANI MPILA Defny Silvia Wewengkang Deliyana Lanti Desye Nurmalita Tanan Devianitta Sarapi Dian Pratiwi Dian Pratiwi Dina Rombot Dolongtelide, Jeclin Inebel Donald Andreas Chandra Dos D. L. E. Br Siagian Edward Nangoy, Edward Eka Mulya Muthalib Ekawati Tallei, Trina Ellen Manampiring, Aaltje Elly Suoth Endang Triastuti Engka, Joice Nancy ERLADYS MELINDAH RUMONDOR, ERLADYS MELINDAH Eva M. Mantjoro Farhan A. Bidullah Fatima Fatima Fatmawaty Badaruddin Feti Fatimah Feybe Ireine Kumayas Fona Budiarso Frans S. M. Mintardjo Frederika Filly Toad Frenly Wehantouw Frisky S. Badi, Frisky S. Friyan Criscanus Manopo Gabriela Clementine Ranti Gani Jumaat Gani, Maria A Gani, Maria A. Gayatri Citraningtyas Gazali Gazali Gemi Nastiti Gerungan, Yizreel Y. GONI, BRIGITA CHRISTANIA AURORA Gosal, Leonardo Delvin Grace Debbie Kandou Grenshannya Anasthasya Pua Greta J. P. Wahongan Gustaaf A. E. Ratag Hamdiyah Hamzah Hamidah Sri Supriati Hanna Elga Sulu Hariyanto, Yuanita Amalia Hasan, Puput Herawati Hasan, Puput Herawati Said Hasanuddin, Israyati R. Hebber, Tri Heedy M Tjitrosantoso Henki Rotinsulu, Henki Hermina Budiarso, Fona Dwiana Herny E.I. Simbala Hosea Jaya Edy I Dewa Ayu Accyuta Kirana I Dewa Ayu Prabawati I Putu Andika Arianta Inda V. M. Rawung, Inda V. M. Intan Baiduri Irawan Yusuf Irawan Yusuf Irma Antasionasti Irma Antasionasti Irma Antasionasti Irma Febrianti Wahongan Irma Tristanti Iswanto Toling Jainer P Siampa Jainer Pasca Siampa Jane Tahulending Jayanto, Imam Jeane Mongi Jeanette I. Ch. Manoppo Jimmy Posangi Jimmy Posangi Jimmy Posangi Jimmy Posangi John Socrates Kekenusa John Socrates Kekenusa Johnson Kepel, Billy Jootje M. L. Umboh Josepin P Konda Julianri Lebang Julianri Sari Lebang Julianri Sari Lebang Jurike Kaunang Kairupan, Tara S. Kalalo, Marko Jeremia Kalalo, Tekla Kamaruddin Kamaruddin Kambey, Debora F. Kamu, Priska Eliasa Kapondo, Gwendolyn L. Karamoy, Eunike M. Karauwan, Indri G Kawengian, Shirley ES. Keintjem, Brigieta Kepel, Billy Kepel, Billy Johnson Kepel, Regina E.M. Kezia Pangemanan Kissi Parengkuan Konda, Josepin P Kuliahana, Ana Kumala Dewi, Citra Kumowal, Selphina Lady zha-zha Luntungan Lampus, Christania Landy A. Ch Lolaen LAPIAN, ALDA JULISTY GABRIELA Lasut, Misella R. C. Lasut, Misella Regina Laurencia Diva Bernadette Artantyo Lengkong, Cheisy Anastasya Gratia Lidya Togelang Lidya Valda Mamoto Lisa Kepel M Fadhel Nurmidin M. Pertiwi, Junita Ma'ruf, Nurul Qalbiyyah Makatambah, Venila Malino, Angeline Priscillia Mambo, Christi Diana Manampiring, Aaltje Ellen Mansur, Nurfauziah Mantjoro, Eva M. Maria Immaculata Tangkau Marina Mamarimbing Marko Jeremia Kalalo Marko Jeremia Kalalo Martha Marie Kaseke Maulydia, Nur B. Max R.J. Runtuwene Mega Fatimah Rosana Meilani Jayanti Melkianus, Brando Miranti Aike Wemay Mohtar, Khumairah Momuat, Heski Deswan Monalisa Karinda Morgen Ekaputra Pangaila Muh. Rifaldi Latarang Muharli Qadri Kanon Mutiara Cantika Mutiara Ramadani Mutmainah Mutmainah Nainggolan, Ivana C. Naray, Gueen L. G. Natasya Ester Rebeca Tamahiwu Natsir, Rosdiana Ni Wayan Dianti Niode, Nurdjannah Jane Nova Hellen Kapantow Novelina Irianti Damanik Nurdjannah J. Niode Nurfitriani Nurfitriani, Nurfitriani Nurhamidin, Anastasia P.R. Oksfriani Jufri Sumampouw Olivia C. Mulalinda Olivia G. Mokolensang, Olivia G. Olvie Datu Olvie Syenni Datu Olvie Syenni Datu Olvie Syennie Datu Omega Agral Owu, Nadya M. PANI, PUTRI MARGARETHA GLAUDY Pantow, Natalia M. Patrick Muljono Patrick Muljono, Patrick Paukiran, Degol Paulina Yamlean Paulina yamlean Paulina Yamlean Pehino, Albrita Pendong, Christa Hana Angle Pertiwi, Junita Maja Poluakan, Ricko Johanes Prasetio, Nathanael F. Prayoga, Deshanda Kurniawan Priska F. Umboh Puasa, Novanda S. Purwanto, Diana Shintawati Putri, Ida A. R. Putri, Prisna Aswarita Rahadian, Rifky Aditya Rahmad Dian Rambi, Christani I J Ratte, Titah Amelia Ratu, Belinda D. P. M. Ratulangi, Andrew Johanes Refa Riski Aulia Regina Masengi, Angelina Stevany Rengur, Zul Aini Rifqi Adithya Rika Wulandari Rimporok, Aprilia Rinaldi Idroes RINI Rio Windy Sondakh Ristanti Pratiwi Rizya Marchilia Mamahit Rotinsulu, Sarah Rotty, Linda WA. Rufi'i Rumagit, Tjandra A. RUMALUTUR, CHRISTHALIA IEWANDA Rumangu, Chrisa P. Rundengan, Gerald Sahi, Makbul Renaldi Salsabila, Febrisa Saragih, Nathin Loria , Sarah Mariana Pattuju Saranita V. G. Polii sari sakinah Sherly Dawile Shintia, Arini Siampa, Jainer P Siampa, Jainer Pasca Siboantua Broolin Simanjuntak Siringo-Ringo, Aurian Fricilia Siti Suhartini Sitti Romlah Solang, Jonathan Adrian Natanael Sri Sudewi Sri Sudewi, Sri Sriwaningsih S Nahe Stevana F.A. Paat Sugeha, Inti Rizcy Sari Sumual, Acika Syifa Katili Talla, Tamrin Tallei, Trina Ekawati Tania, Adinda Dwi Tendean, Lydia Estelina Naomi Tengke, Feniea Wulan Agaype Terence Kanzil Titah Amelia Ratte Tjandra A. Rumagit Tjandra, Regina F. Trina E Tallei Trina E Tallei Trina E. Tallei, Trina E. TRINA EKAWATI TALLEI Trina Ekawati Tallei Trina Ekawati Tallei Trina Tallei Trina Tallei Trina Tallei Turalaki, Grace Lendawati Amelia V. Lomotu, Dinda Vanessa J. T. Seran, Vanessa J. T. VERY LONDA Vibrila Berliana Djanas Watania, Marcela Wawo, Arsianita Ester Welong Seftian Surya Weny I Wiyono Weny Wiyono Widdhi Bodhi Widdhi Bodhi Widdhi Bodhi Widdhi Bodhi widdhi Bodhi Widdhi Bodhi Widhi Bodhi Widya Hana Putri Gerung Wijaya, Puspita Windy Sumarauw Wulan P. J. Kaunang Wulan Palilingan, Wulan Wulan Tuerah Wungouw, Herlina Ineke Surjane Wungouw, Herlina IS. Yamani, Gasim Yohanes Adithya Koirewoa Yulia Maria Jarut Yulianty Sanggelorang Yuyun Andriani Agus Zahra Zahra Zainal Abidin Zulfikri Zulfikri, Zulfikri