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Gut Microbiota Profile in Endometriosis Patients at Dr. M. Djamil Padang, West Sumatra, Indonesia Haviz Yuad; Utama, Bobby Indra; Lipoeto, Nur Indrawaty; Putra, Andani Eka
Andalas Obstetrics And Gynecology Journal Vol. 9 No. 1 (2025)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.9.1.134-145.2025

Abstract

Objective: Endometriosis (EMS) is a chronic gynecological condition affecting up to 10% of women worldwide. The pathogenesis associated with hormonal imbalance, immune dysregulation, and changes in the gut microbiota. Gut microbiota dysbiosis may drive chronic inflammation that contributes to the progression of EMS. This study hypothesizes that certain gut microbiota profiles are associated with these factors in EMS patients, potentially developing non-invasive diagnostics and more targeted therapies. Methods: This is an observational analytical study with a case-control design conducted at Dr. M. Djamil Padang General Hospital in May 2024 with a sample size of 44 people. Case samples were women aged 18–40 years who were diagnosed with endometriosis based on the inclusion criteria, while the control group were women of reproductive age without a diagnosis of endometriosis. Data testing used the Next Generation Sequencing method, microbiome analysis test, and SPSS test. Results: The majority of EMS patients were aged 30-37 years, married, and had Nullipara obstetric status. In addition, the gut microbiota was dominated by the phyla Bacteroidetes and Firmicutes. Conclusion: There is a relationship between gut microbiota dysbiosis and the incidence of endometriosis, namely an increase in Bacteroidetes and a decrease in Firmicutes Keywords: endometriosis, dysbiosis, bacteroides, firmicutes, gut microbiota
The Efficacy of Dequalinium Chloride Against Vulvovaginitis in Pregnancy Mila Permata Sari; Djanas, Dovy; Putra, Andani Eka
Andalas Obstetrics And Gynecology Journal Vol. 9 No. 1 (2025)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.9.1.49-62.2025

Abstract

Introduction: Vulvovaginitis affects pregnancy outcomes and managing it involves the use of metronidazole or nystatin, which can cause local or systemic side effects. Dequalinium chloride (DQC) is suggested as a new treatment option for vulvovaginitis during pregnancy with minimal side effects. Objective: To compare the effectiveness of dequalinium chloride (DQC) versus metronidazole + nystatin (MN) in treating vulvovaginitis in pregnant women during their second and third trimesters. Method: A single-blind randomized controlled trial was conducted at the Andalas Public Health Center, Padang, from January to May 2024. Pregnant women in their second and third trimesters, meeting the inclusion and exclusion criteria, were enrolled. The participants were randomly assigned to either the DQC or MN group. Vaginal swabs were taken before and after a 6-day treatment, and PCR analysis was performed. Results: Eighteen patients were treated with DQC and MN. Most participants had bacterial vaginosis (DQC 78%, MN 83%), with half having candidiasis (DQC and MN 50%) and fewer cases of trichomoniasis (28% in both groups). Significant improvements were seen in the DQC group for symptoms (p=0.000), Gardnerella vaginalis (p=0.035), and Candida albicans (p=0.021). In the MN group, significant improvements were noted for symptoms (p=0.000) and Gardnerella vaginalis (p=0.002). No significant differences were observed between the groups for symptom resolution or microbial reduction. Conclusion: DQC is as effective as metronidazole + nystatin in treating bacterial vaginosis and candidiasis in pregnancy. Keywords: Vulvovaginitis, pregnancy, dequalinium chloride, metronidazole, nystatin
Lactobacillus species and Clamydia trachomatis characterisation in premature rupture of membranes and normal pregnancy Prima Indra; Roza Sri Yanti; Putra, Andani Eka
Andalas Obstetrics And Gynecology Journal Vol. 9 No. 1 (2025)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.9.1.95-104.2025

Abstract

The cause of premature rupture of membranes (PROM) remains uncertain. The vaginal microbiota might possess a significant influence on the vaginal environment and can impact the overall health of the female reproductive system. Objective: This study aims to determine the difference in the concentration of lactobacillus crispatus, lactobacillus vaginalis, and chlamydia trachomatis between patients with premature rupture of membranes and normal pregnancies; Methods: This study is an analytic study with a case-control design that was conducted at Dr. M. Djamil Hospital Padang during the period January 2024 to June 2024. This study performed DNA isolation using conventional PCR; Results: Lactobacillus crispatus, Lactobacillus vaginalis, and Chlamydia trachomatis reached 42,124,873.86/mL, 179.41/mL, and 287,062.30/mL, respectively in preterm rupture of membranes patients. In normal pregnancy, Lactobacillus crispatus, Lactobacillus vaginalis, and Chlamydia trachomatis reached 75,717,348.80/mL, 704,147.66/mL, and 0/mL, respectively. A significant difference in Lactobacillus vaginalis concentrations was found between the case and control groups, with the control group having a greater average concentration (28.88) compared to the case group (36.13), with a p-value <0.018. The case group had a significantly greater concentration of Chlamydia trachomatis (37.50) compared to the control group (27.50), with a p-value <0.001. The case and control groups had significantly different lactobacillus crispatus concentrations. The case group had 24.88 times less lactobacillus crispatus than the control group, which had 40.13 times more, with p-value <0.001; Conclusion:  In pregnant women, an increase in Chlamydia trachomatis and a reduction in Lactobacillus crispatus and Lactobacillus vaginalis can be an indicator of probable PROM. Keywords:  Lactobacillus vaginalis, chlamydia trachomatis, Lactobacillus crispatus, PROM, normal pregnancy.
Comparison of Vaginal Microbiota Profiles in Patients With Endometriosis and Without Endometriosis Sef Zani Meria; Yuad, Haviz; Putra, Andani Eka
Andalas Obstetrics And Gynecology Journal Vol. 9 No. 1 (2025)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.9.1.74-85.2025

Abstract

Introduction: Endometriosis is a complex gynecological disease affect 10% of women in reproductive age, involving inflammation and immune regulation. Diversity of vaginal microbiota is an integral part of vaginal microecology with vaginal anatomy, endocrine system and local vaginal immunity. The disruption in microecology, dysbiosis, is known to trigger inflammatory diseases. The relationship between vaginal dysbiosis and endometriosis remains controversial Objective: To determine the comparison of vaginal microbiota profiles in patients with and without endometriosis Method: The study was case-control design on patients with and without endometriosis at Dr. M. Djamil Hospital, conducted from December 2023 to June 2024 at Dr. M. Djamil Hospital Polyclinic and the Center for Infectious Disease Diagnostic and Research Laboratory. The diagnosis of endometriosis was confirmed via ultrasonography and histology. Microbiota were measured with miSeq Illumina Next Generation Sequencing. A comparison of microbiota profiles was carried out using the Mann-Whitney test. Results: There were 40 respondents, with endometriosis (n=20) and without endometriosis (n=20). At the phylum level, the vaginal microbiota profile in the endometriosis group was dominated by Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, while in without endometriosis group dominated by Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. At the genus level, the endometriosis group was dominated by Lactobacillus sp., Desulfovibrio sp., Flavobacterium sp., and Gardnerella sp., while without endometriosis group were Lactobacillus sp., Prevotella sp., Desulfovibrio sp., and Bifidobacterium sp. There was no difference in the abundance of microbiota (p>0.05) Conclusion: There was no difference in the abundance of microbiota between endometriosis group and without endometriosis group.   Keywords: Endometriosis, dysbiosis, vaginal microbiota, Lactobacillus, Flavobacterium, Firmicutes, Proteobacteria
The Enhancement of COVID-19 Severity on Coagulation Markers: a Cross-sectional Study in West Sumatra, Indonesia Febrianti, Ika Kurnia; Putra, Andani Eka; Raveinal, Raveinal; Elliyanti, Aisyah
Majalah Kedokteran Andalas Vol. 47 No. 2 (2024): MKA April 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i2.p139-147.2024

Abstract

Objective: This study aimed to determine the description of the coagulation marker and how it implicates COVID-19 patients based on clinical outcomes in the form of enhancement severity and death. Method: The subjects were collected from patients with positive COVID-19 RT-PCR with medical records including demographic data, and laboratory results such as hemoglobin, hematocrit, leukocytes, platelets, coagulation markers (D-dimer, PT, and APTT), and patients outcomes post-treatment. Statistical analysis is performed by using chi-square and Mann-Whitney tests were considered statistically difference level of significant between variables. Result: The COVID-19 patients is classified into mild group with 27 subjects  (44.2%) and moderate-severe group 34 subjects (55.8%). Leukocytes, D-dimer, and PT levels significantly differed between mild and moderate-severe groups. All coagulation markers had a symptomatic effect on Covid-19 patients, increased D-dimer and prolonged prothrombin time in adult COVID-19 patients tend to be followed by the enhancement of severe symptoms and death
Distribusi Mutasi Asp614Gly pada Spike Protein SARS-CoV-2 di Sumatera Barat Agustian, Dede Rahman; Putra, Andani Eka; Arisanty, Dessy
Majalah Kedokteran Andalas Vol. 47 No. 2 (2024): MKA April 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i2.p163-172.2024

Abstract

Objective: This study aims to map the distribution of the Aspartate 614 to Glycine (Asp614Gly) mutation in the spike protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) circulating in West Sumatra, to understand the extent of its spread within the local population. Methods: RNA samples were taken from 98 confirmed COVID-19 cases across various area in West Sumatra. Genetic amplification and next-generation sequencing (NGS) were performed to identify the presence of the Asp614Gly mutation. The frequency of mutations was analyzed using bioinformatics software. Results: Out of the 98 samples analyzed, approximately 97% exhibited the Asp614Gly mutation. The geographic distribution of this mutation showed significant variation among the districts of West Sumatra, with the highest prevalence in urban areas. Conclusion: These findings indicate that the Asp614Gly mutation is highly prevalent in West Sumatra. Associated with increased virus transmissibility, this mutation dominates the circulating virus strains in the region, highlighting the need for ongoing genetic surveillance to guide public health interventions and vaccination strategies.
Prevalensi Infeksi Cytomegalovirus (CMV) Berdasarkan Jenis Sampel dan Karakteristik Pasien Balita di Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi Universitas Andalas Tahun 2023–2024 Tresnaningtyas, Sekar Asri; Putra, Andani Eka; Farashanda, Meysha; Fahma, Siskalil; Hanif, Monica Mulnia
Medika: Jurnal Ilmiah Kesehatan Vol 5 No 2 (2025): Medika: Jurnal Ilmiah Kesehatan
Publisher : Universitas Nahdlatul Ulama Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69503/medika.v5i2.1126

Abstract

Infeksi Cytomegalovirus (CMV) merupakan salah satu infeksi virus yang berpotensi serius pada anak balita, terutama karena potensi komplikasi jangka panjang. Penelitian ini bertujuan untuk mengetahui prevalensi CMV berdasarkan jenis sampel (urin dan darah) serta karakteristik pasien balita yang diperiksa di Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi Fakultas Kedokteran Universitas Andalas selama tahun 2023 hingga 2024. Penelitian dilakukan secara deskriptif analitik terhadap 87 data pasien anak usia 0–28 bulan. Analisis statistik meliputi uji Kruskal-Wallis, chi-square, dan McNemar. Hasil menunjukkan bahwa sebagian besar pasien memiliki hasil positif CMV pada kedua spesimen, yaitu urin dan darah (42,5%), diikuti oleh positif hanya pada urin (18,4%), hanya pada darah (14,9%), dan negatif pada keduanya (24,1%). Tidak terdapat hubungan signifikan antara jenis kelamin atau usia dengan hasil CMV. Uji McNemar menunjukkan tidak ada perbedaan bermakna antara hasil pemeriksaan urin dan darah (p = 0,710). Dapat disimpulkan bahwa baik urin maupun darah memberikan hasil yang sebanding, meskipun dengan konsistensi terbatas.
Pengaruh Pemberian Es Krim Dadih Terhadap Perubahan Jumlah Lactobacillus Fermentum Pada Feses Anak Obesitas Wahyuni, Yosha Putri; Sulastri, Delmi; Eka Putra, Andani
Majalah Kedokteran Andalas Vol. 47 No. 3 (2024): MKA July 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i3.p321-329.2024

Abstract

Tujuan: Tujuan penelitian ini untuk melihat pengaruh pemberian es krim dadih terhadap jumlah Lactobacillus fermentum anak obesitas. Metode: Penelitian ini merupakan penelitian ekperimental dengan desain pre-post test with control group. Responden penelitian adalah 24 orang anak obesitas yang masuk kriteria inklusi dan ekslusi yang dibagi menjadi dua kelompok yaitu kelompok perlakuan adalah yang diberikan es krim dadih dan kelompok kontrol adalah yang diberikan es krim tanpa dadih. Jumlah Lactobacillus fermentum diperiksa di laboratorium mikrobiologi Fakultas Kedokteran Universitas Andalas Padang dengan metode real- time PCR. Data dianalisis dengan menggunakan uji Dependent sample t-test. Hasil: Hasil penelitian didapatkan rata-rata jumlah Lactobacillus fermentum sebelum intervensi pada kelompok perlakuan 2,59 ± 1,18 log cfu/gr dan setelah intervensi 3,74 ± 0,75 log cfu/gr, pada kelompok kontrol sebelum intervensi 3,47 ± 1,27 log cfu/gr dan setelah intervensi 3,21 ± 0,41 log cfu/gr. Terdapat perbedaan yang signifikan jumlah Lactobacillus fermentum sebelum dan setelah diberikan intervensi pada kelompok perlakuan dan tidak terdapat perbedaan pada kelompok kontrol. Kesimpulan: Pada penelitian ini dapat disimpulkan terdapat pengaruh pemberian es krim dadih terhadap jumlah Lactobacillus fermentum pada feses anak obesitas.
Hubungan infeksi Human papillomavirus dengan histopatologi serviks wanita : sebuah narrative review Kaisar , Kevin Florentino; Rahmi, Wahida; Delfilaura, Asyifa; Putra, Andani Eka
Majalah Kedokteran Andalas Vol. 48 No. 4 (2025): MKA October 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v48.i4.p466-478.2025

Abstract

Latar Belakang: Kanker serviks menempati urutan keempat terbanyak secara global dan kedua pada wanita, dengan Human papillomavirus (HPV) sebagai faktor risiko utama. Infeksi HPV, terutama tipe onkogenik seperti HPV16 dan HPV18, dapat menyebabkan perubahan histopatologi serviks dari lesi intraepitel skuamosa rendah hingga kanker invasif. Tujuan: Mengetahui hubungan infeksi HPV dengan gambaran histopatologi serviks menggunakan pemeriksaan sitologi, khususnya pap smear. Metode: Pendekatan narrative review, database PubMed, ProQuest, Cochrane, dan Google Scholar periode 2015–2020 dengan metode PRISMA dan kriteria inklusi spesifik. Hasil: Menunjukkan sebagian besar kanker serviks berasosiasi dengan infeksi HPV, dengan variasi tipe dan prevalensi pada tiap studi. Pemeriksaan kombinasi sitologi dan deteksi genom HPV memiliki sensitivitas tinggi dalam skrining kanker serviks. Kesimpulan: Temuan ini menegaskan pentingnya deteksi dini HPV untuk pencegahan progresi displasia menjadi kanker invasif.
The Efficacy of Application of Oral Antibiotic Loaded Polymethyl Methacrylate Against Staphylococcus aureus Rahmadian, Rizki; Edison, Ebill Fuji; Kaharudin, Kaharudin; Irrahmah, Miftah; Putra, Andani Eka
Indonesian Journal of Multidisciplinary Science Vol. 2 No. 9 (2023): Indonesian Journal of Multidisciplinary Science
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/ijoms.v2i9.543

Abstract

Chronic osteomyelitis is still common in the developing world and presents a continuing therapeutic challenge. Local antibiotic therapy is performed as the first stage treatment algorithm for 4 to 8 weeks. Given the high patient morbidity, mortality, and economic burden caused by osteomyelitis and its treatment, it is important to find another effective and efficient treatment.  Intravenous antibiotics and polymethylmethacrylate (PMMA) combination have shown effectiveness, but the potential of oral antibiotics and PMMA combination is still rare and is debatable. It aimed to compare efficacy among oral antibiotic-loaded PMMA in many concentrations (5%, 10%, 20%) and controls (0%) against Staphylococcus aureus. This experimental study was conducted in the Microbiology Department of Andalas University. The object was S. aureus. We used ten oral antibiotics and compared among its many concentrations (5%, 10%, 20%) and controls (0%).  Data were analyzed by analysis of variance (ANOVA) test. The lowest counts of S. aureus were found in ciprofloxacin 20%, followed by tetracycline 10%, erythromycin 5%, rifampicin 10%, ofloxacin 20%, clindamycin 20%, amoxiclav 20%, cefixime 5%, cotrimoxazole 20%, and chloramphenicol 20% consecutively. All oral antibiotics loaded PMMA in each concentration might reduce the number of colonies up to 90%. A study in 2013 revealed that a combination of several antibiotics and PMMA was proven to be effective. The oral antibiotics loaded PMMA in various concentrations have a significant bactericidal effect on the growth of S. aureus and able to reduce the number of S. aureus.
Co-Authors - Johan Abdiana Abdiana, Abdiana Adang Bachtiar Ade Asyari Afida Razuna Ave Afrizal Agustian, Dede Rahman Aisyah Elliyanti Akmal Djamaan Alioes , Yustini Amelin, Fitrisia Amirah Zatil Izzah Amrin Alkamar Anavelda, Aura Putri Annisa Widi Rizkia Antonius, Puja Agung Arfai Arfai Arfiandi Arfiandi Ariani, Tutty Arni Amir Arni Amir arniamir, Arni Amir Asri, Ennesta Bari'ah, Puti Asma Basyir, Vaulinne Besri, Hanifa Zahra Bobby Indra Utama Cimi Ilmiawati, Cimi Darfioes Basir Deddy Saputra Dede Agustian Rahman Defni, - Defrin, Defrin Delfican Delfican Delfilaura, Asyifa Delmi Sulastri Densi Selpia Sopianti Densi Selpia Sopianti Desmawati Desmawati Dessy Arisanty Desy Nofita Sari Djanas, Dovy Edison, Ebill Fuji Efrida Efrida Eka Fithra Elfi, Eka Fithra Elizabeth Bahar Elizabeth Bahar Elmatris Sy Eryati Darwin Eti Yerizel Fahma, Siskalil Farashanda, Meysha Farras, Umar Al Fathiyyatul Khaira Fauzul Azhim Febrianti Ika Kurnia Feby Resicha Fenty Anggraini Finny Fitry Yani Fiona Dewanti Firdawati, Firdawati Fitri Anggraini, Fitri Fitri Nova, Fitri Fitri, Azdiana Fitrina, Dewi Wahyu Gardenia Akhyar Gestina Aliska Hafni Bachtiar Hanif, Monica Mulnia Hanif, Rayhendra Hardisman Hari, Dewi Gulyla Hasmiwati Haviz Yuad Haviz Yuad Helmizar Hermawan, Budi Junio Hidayanti, Rahmi Husna Yetti Husna, Annisa Tamara Husni Husni Ida Parwati Ilmiawati, Ilmiawati Irrahmah, Miftah Irvan Medison Jamsari Jamsari Kaharudin Kaharudin Kaisar , Kevin Florentino Kosno Suprianto Leona, Denada Florencia Linosefa Linosefa Liza Fitria M. Alfian Agustian Mahata, Liganda Endo Malinda Meinapuri Marlina . Megariani Megariani Menkher Manjas Mentari Faisal Putri Mila Permata Sari Muhammad Rayhan Firdaus Muhammad Reza Nelmi Silvia Netti Suharti Netti Suharti, Netti Nia Ayuni Putri Nila Kasuma Novita Ariani Nur Indrawaty Lipoeto Nurhayati, Nurhayati Oky Masir Prima Indra Putri Rizki Fitriani Putri, Mentari Faisal Rahmadian, Rizki Rahmat Syawqi Rahmi Lestari Rahmi, Wahida Ramadhan, Mario Arya Ranne Balqis RAVEINAL RAVEINAL Rheta Elkhaira Rinang Mariko Rinang Mariko Rita Hamdani Rizanda Machmud Rosfita Rasyid Roslaili Rasyid Roza Sri Yanti Russilawati, Russilawati Rustini Rustini Salmiah Agus Salmiah Agus Saptino Miro Sary, Rizka Yulia Sef Zani Meria silviayolanda, Silvia Yolanda Sinai, Nadiva Kezia Sosmiarti Sosmiarti Sriyanti, Roza Suharti , Netti Suzana Devi Syahrul, Muhammad Zulfadli Syamel Muhammad Tarigan, Herri Novita Taufiqulhakim, Muhammad Rafi Tresnaningtyas, Sekar Asri Tri Anggraini, Fika Tyas, Syanindhita Wikanthining Utama, Bobby Indra Utami, Refi Amalia Wahyu Zikra Wahyuni, Yosha Putri Wenny Nursa Octarina Wiwik Andriani Yefri Zulfiqar Yessi Widya Putri Yuad, Haviz Yufri Aldi Yufri Aldy Yuliati Shafan Nur Yusri Dianne Jurnalis