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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Ilmu dan Teknologi Kelautan Tropis Hemera Zoa Media Veteriner Majalah Ilmiah Peternakan Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Reproduksi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology ZOO INDONESIA Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal ARSHI Veterinary Letters Jurnal Kedokteran Hewan Current Biomedicine

Arief Boediono

Bogor Agricultural University

Author-ID : 232094

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

Published : 83 Documents Claim Missing Document

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The use of CR1aa for ovine in vitro embryo production Yulnawati .; M.A Setiadi; A Boediono
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this study was to investigate the capacity of CR1aa as a simple medium for maturation, fertilization and culture of ovine embryo in vitro. Oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 as control or CR1aa as treatment medium. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 μg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Oocytes were incubated in 5% CO2 incubator, 38˚C for 24 h. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg /ml heparin. After 18 h in vitro fertilization, oocytes were cultured in TCM-199 or CR1aa medium, both supplemented with 5% FBS, 5 mg/ml insulin and 100 IU/ml penicillin streptomycin. Results showed that the highest maturation rate was found in TCM-199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 and CR1aa medium (39.45% vs 50.94%). In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM-199 as maturation medium and CR1aa as fertilization and culture medium. Key Words: CR1aa, TCM-199, Embryo, Ovine

Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production M Imron; A Boediono; I Supriatna
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation. The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number

Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams Rahmat Hidayat; T. Toharmat; A. Boediono; I.G. Permana
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Contribution of Garut breed sheep as protein resources is not optimal yet. Acceleration of population growth such as increasing of female offspring is neccesary. This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) – (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows: RN0= basal ration (PKAR +14) without fish oil, RNI= basal ration (PKAR +14) with 3% fish oil, RB0= base ration (PKAR +40) without fish oil, RBI= base ration (PKAR +40) with 3% fish oil, RA0= acid ration (PKAR -40) without fish oil, and RAI= acid ration (PKAR -40) with 3% fish oil. The rations contained 150 ppm of zinc and were offered to 18 of Garut rams. The result indicated that blood pH, pCO2, and pO2 were not affected by PKAR and fish oil supplementation, but cHCO3 and cBase were affected (P<0.05). PKAR and fish oil supplementation affected (P<0.01) plasma Mg and S, but did not affect K, Na, Zn and Cl. Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern. In conclution, PKAR affected cBase, cHCO3, concentration of Mg and S plasma and semen pH of Garut rams. The result suggested that PKAR could be applied to manipulate physiological condition and semen pH. Key Words: PKAR, Physiological Condition, Semen, Garut Ram

Spatial learning and memory of young and aging rats following injection with human Wharton’s jelly‐mesenchymal stem cells Berry Juliandi; Wildan Mubarok; Dian Anggraini; Arief Boediono; Mawar Subangkit; Indra Bachtiar; Harry Murti; Kelvin Yaprianto; Boenjamin Setiawan
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64734

Human Wharton’s jelly‐mesenchymal stem cells (hWJ‐MSC) are an emerging potential source of stem cells derived from the umbilical cord. Previous studies have shown their potential as treatment for traumatic brain injury and Parkinson’s disease. However, no study has yet investigated the effect of hWJ‐MSC injections in countering spatial learning and memory impairment in aging rats. The effect of hWJ‐MSC injection on young rats is also unknown. The objective of this research was to analyze the effect of an hWJ‐MSC injection on spatial learning, memory, density of putative neural progenitor cells (pNPC), and neuronal apoptosis in the dentate gyrus (DG) of young and aging rats. Injection of hWJ‐MSC did not change spatial learning and memory in young rats until two months post‐injection. This might be due to retained pNPC density and neuronal apoptosis in the DG of young rats after injection of hWJ‐MSC. In contrast, injection of hWJ‐MSC promoted both spatial learning and memory in aging rats, a finding that might be attributable to the increased pNPC density and attenuated neuronal apoptosis in DG of aging rats during the two months post‐injection. Our study suggests that a single injection of hWJ‐MSC might be sufficient to promote improvement in long‐term learning and memory in aging rats.

EXPRESSION PROFILE OF SEX DETERMINATION GENE, BIOREPRODUCTION, PHENOTYPE, AND LOCOMOTORY PERFORMANCES OF OLIVE RIDLEY, Lepidochelys olivacea INDUCED BY DIFFERENT INCUBATION TEMPERATURE Alfred O. M. Dima; Dedy D. Solihin; Wasmen Manalu; Arief Boediono
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 7 No. 1 (2015): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Sex determination in turtle species is not only based on genotype, but also rely on the incubation temperature. In addition, sexual differentiation takes place during the thermo-sensitive period (TSP). This study was conducted to determine the effects of incubation temperature on sex expression profile of determination gene, bioreproduction, phenotype, and locomotory performances of olive ridley turtle hatchlings. Fertile eggs incubated at two temperatures, namely feminine temperature (30-33°C), and masculine temperature (26-27°C). Value of cycle threshold (CT) measured during TSP, i.e 23-25 embryonic development stage, and after TSP, i.e 26-27 embryonic development stage using real time PCR techniques. Comparison of gene expression at both incubation temperatures were analyzed by ANOVA, and Student’s t test. Hatchling bioreproduction and phenotype measurement consist of the incubation period, embryo growth, morphometrics, and locomotori performances hatchlings were analyzed with regression analysis and Student’s t test. The results showed expression of both aromatase and Rspond 1 genes (which plays a role in ovarian differentiation) after the TSP that incubated at feminine temperature higher and different with masculine temperature. In conjunction with the bioreproduction and phenotype, the incubation period of feminine temperature shorter than that of masculine. Likewise, growth of the embryo of feminine temperature was faster than that of masculine. Incubation at feminine temperature significantly affect to carapace width, length and width of the plastron, long flippers and rear arms, long neck, and the frequency of the swing flippers. Keywords: thermo-sensitive period (TSP), gene expression, phenotype, Lepidochelys olivacea

Kualitas, Kemampuan Implantasi dan Viabilitas in-vivo Embrio Mencit (Mus muculus) Galur Swiss Webster Setelah Pembekuan Dengan Metode Vitrifikasi Madihah Madihah; Hartanti Kusumaningtyas; Arief Boediono; Sony H. Sumarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2618

Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.

Konsentrasi dan Kualitas Spermatozoa Kucing Domestik (Felis catus) yang diambil dari Epididymis dan Ductus deferens setelah Preservasi pada Suhu 4oC Cutnya’ Shaliran Nazlie (Alm); Iman Supriatna; Srihadi Agungpriyono; Arief Boediono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.2820

The aim of this study was to evaluate the concentration, progressively motile, and percent live sperm in the various regions of epididymis and ductus deferens after preservation at 4oC. Epididymis and ductus deferens were collected from 21 epididymis and ductus deferens of domesticated cat (Felis catus) by castration. One testicle of pair (control testicle) was analyzed at the day of castration, while the other testicle of the pair was stored at 4oC up to 7 days. The sperm concentration, percentage of sperm motility and live sperm were examined daily until day-7 of preservation. The sperm concentration was higher (p<0.05) in cauda epididymis (23.99x106 sperm/ml) and ductus deferens (25.42x106 sperm/ml) than caput (11.51x106 sperm/ml) and corpus epididymis (14.82x106 sperm/ml). The percentage of sperm motility and live sperm decreased (p<0.05) during preservation period. However, the percentage of motile (11.33 to 16.00 %) and live (15.05 to 20.20 %) sperm could be found in preserved epididymis and ductus deferens up to day-7. These results show that motile and live sperm can be collected from cat’s epididymis and ductus deferens up to day 7 after preservation at 4oC.

Follicular Development of Aged Rats Ovarian Injected Human Umbilical Cord Mesenchymal Stem Cells Resti Rahma Dianti; Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; Aryani Sismin Satyaningtijas; Adi Winarto; Arief Boediono
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.6889

Female reproductive system showing the fastest signs of aging. The ovarian aging characterized by a decrease in follicular development. Stem cells are undifferentiated cells and can form a variety of different cells as the foundation of tissues and organs. Previous studies reported that Bone Marrow Mesenchymal Stem Cells (BM-MSCs) transplantation can restore follicular development in damaged ovarian rats. This study aimed to analyze the number of follicular development in aged rats and to analyze the capability of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) to improving follicular development in aged rats. This study used 3 mature rats (4 months old), and 9 nine aged rats (22-24 months old), Spraque Dawley (SD) strain. They were divided into four groups. The first and the second group was mature rats and aged rats without injection. The third and the fourth group was aged rats injected hUC-MSCs dose 106 cells/kgBW and hUC-MSCs dose 107 cells/kgBW. The injection carried out 4 times at 3-month intervals. The parameters observed were follicular development and homing image of hUC-MSCs in ovarian tissue. The results showed that the number of follicular developments in aged rats 22-24 months decreased significantly compared to mature rats 4 months old. Injection of hUC-MSCs at dose 106 cells/kgBW and 107 cells/kgBW did not increase follicular development in aged rats. hUC-MSCs did not found in ovarian tissue. It could be concluded that aged rats 22-24 months old no longer productive indicated from the number of follicular developments and corpus luteum decreased. The injection of hUC-MSCs intravenously did not indicate an improvement of follicular development in aged rats 22-24 months old.

OVARY DEVELOPMENT, FSH AND LH GENES EXPRESSION OF INDONESIAN LEAFFISH, Pristolepis grootii (Bleeker, 1852), INJECTED WITH LUTEINIZING HORMONE-RELEASING HORMONE ANALOG Muslim Muslim; Agus Oman Sudrajat; Muhammad Zairin Jr.; Muhammad Agus Suprayudi; Arief Boediono; Iis Diatin; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.69-77

Indonesian leaffish, Pristolepis grootii (Bleeker, 1852), is an undomesticated freshwater fish species native to the rivers, flooded swamps, and tributaries of Indonesia. The fish is mainly captured for consumption. In order to prevent its extinction and supply its growing demands, the artificial breeding of the fish should be developed. The purpose of this study was to determine the optimum dose of luteinizing hormone-releasing hormone analog (LHRHa) for stimulating the female P. grootii gonadal development at a dosage of 0, 1, 10, and 50 µg kg-1 of fish. Female fish (20.0 ± 0.6 g) were adapted for 30 days in the rearing environment and then separated into 12 aquariums with six fish per aquarium. Fish were then reared for another 21 days and fed with Tubifex sp. The LHRHa injection was conducted twice on day-7 and 14. Fish bodyweight, gonadosomatic index, gonad histology, blood estradiol-17â, and FSH-â and LH-â gene expression were evaluated at day 0, 7, 14, and 21. The results showed that the injection of the LHRHa hormone stimulated the development of fish gonads and was better achieved with a higher concentration of LHRHa. The best treatment was observed by the administration of 50 µg kg-1 of LHRHa that produced the fastest development among all treatments. This study demonstrated that the LHRHa induction could potentially stimulate the gonadal development of the newly domesticated fish. To our knowledge, this is the first study that reported the success of the induction of female gonad development in the Indonesian leaffish P. grooti.KEYWORDS:

Sonometri fetus kambing kacang usia 7 minggu hasil superovulasi menggunakan hormon PMSG Ridi Arif; . Andriyanto; Arief Boediono; Adi Winarto; Fadjar Satrija; Wasmen Manalu
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Teknologi superovulasi dapat dimanfaatkan untuk meningkatkan sekresi endogen hormon kebuntingan. Salah satu manfaat dari peningkatan sekresi endogen hormon kebuntingan adalah perbaikan perkembangan lingkungan uterus selama kebuntingan. Kambing Kacang betina sebanyak 8 ekor dan telah dewasa kelamin dengan bobot rataan 22 kg dibagi ke dalam dua kelompok yaitu kelompok kontrol (tidak disuperovulasi) dan kelompok superovulasi menggunakan hormon Pregnant Mare Serum Gonadotropin (PMSG) dengan dosis 15 IU/kgBB. Kambing Kacang percobaan diserentakkan berahinya menggunakan PGF2α sebanyak 2 kali dengan selang 11 hari. Penyuntikan PMSG dilakukan bersamaan dengan penyuntikan PGF2α kedua pada kelompok superovulasi. Setelah berahi, semua kambing dikawinkan secara alami dengan pejantan pilihan. Fetus kemudian diukur secara ultrasonografi (USG) pada usia kebuntingan 7 minggu. Hasil pengukuran menunjukkan kelompok Kambing Kacang hasil superovulasi memiliki ukuran fetus yang lebih panjang daripada kelompok kontrol (P<0.05). Ukuran diameter amnion terpanjang dan terpendek serta tebal dinding uterus terlihat cenderung meningkat pada kelompok superovulasi dibandingkan dengan kelompok kontrol (P>0.05). Kesimpulan penelitian ini adalah superovulasi pada induk Kambing Kacang mampu meningkatkan pertumbuhan fetus sampai dengan usia kebuntingan 7 minggu.

Co-Authors . AULANI’AM . Herdis . Yulnawati A Winarto A.S. Satyaningtijas Abinawanto Adi Winarto Adisti Dwijayanti Adkhilni Utami Adkhilni Utami Adrian Situmeang Adrian Situmeang Adrian Situmeang Adrien Jems Akiles Unitly Adrien Jems Akiles Unitly, Adrien Agus Harsoyo Agus Harsoyo Agus Oman Sudrajat Agus Setiadi Ahmed, Ifty Al Azhar Al Mukhlas Fikri AL-AZHAR AL-AZHAR Alfred O. M. Dima Alif Iman Fitrianto Alif Iman Fitrianto Alimuddin Alkaustariyah Lubis Amrozi Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andri Maruli Tua Lubis Andri Maruli Tua Lubis Andriyanto A Andriyanto Andriyanto ANOM BOWOLAKSONO Arie Adrianus Polim AS Aku, AS Aucky Hinting Aulia Miftakhur Rahman Ayu Mulia Sundari Bambang Kiranadi Batara Sirait Bayu Rosadi Berry Juliandi Bibiana W Lay BIBIANA W LAY Boenjamin Setiawan Boenjamin Setiawan Budiariati, Vista Cahayadi, Sigit Daru Cece Sumantri Chairun Nisa Citra Noviana Cutnya’ Shaliran Nazlie (Alm) D Sudrajat Dedy D. Solihin Diah Nugrahani Pristihadi Dian Anggraini Djaswadi Dasuki Djoko Walujo Dody Dharmawan Trijuno Dondin Sajuthi Dwi Budiono Dwierra Evvyernie Dwiranti, Astari Elpita Tarigan Eni Kusrini EVY AYU ARIDA F Amir F Fathul Farid A. Moeloek Ferry Sandra Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Funahashi, Hiroaki H Maheshwari Hadi, Restu S. Handina Rakhmawati Handina Rakhmawati Harry Murti Harry Murti Harry Murti Harry Murti Hartanti Kusumaningtyas HERA MAHESHWARI Herdis . herdis herdis Heri Sujoko Heru Setijanto I G Permana I Ketut Mudite Adnyana I Ketut Suatha I Supriatna I Wayan Batan I.G. Permana Ichsan Ichsan Iis Diatin Iman Supriatna Indra Bachtiar Indra Kusuma Irma H Suparto Irma Herawati Suparto Irma Suryani Ita Djuwita Ita Djuwita ITA DJUWITA Ita Fauzia Hanoum, Ita Fauzia Ivan Sini Karisma Mardatillah Karisma Mardatillah Kartini Eriani KARTINI ERIANI KARTINI ERIANI Kartiwa, R. Angga Kelvin Yaprianto Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Krido Brahmo Putro Kusdiantoro Mohamad Kusumaningtyas, Hartanti Latifah Kosim Darusman Lea Tarliyah Lindiawati, Riris M Agus Setiadi M Fakhrudin M Imron M Rizal M. Haviz M.A Setiadi Madihah Madihah Maheshwari H Maman Surachman Mas Rizky A.A. Syamsunarno Maula, Yogi Nikmatul Miraprahesti, Retti N. Mohamad Fakhrudin Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Gunawan Muhammad Gunawan Muhammad Gunawan MUHAMMAD RIZAL Muhammad Rizal Muhammad Rizal Muhammad Rosyid Ridlo Muhammad Zairin JR Muhammad Zairin Jr. MULYOTO PANGESTU MULYOTO PANGESTU Muslim Muslim N M D Haq Nastiti Kusumorini Nastiti Kusumorini NASTITI KUSUMORINI Nining Handayani Nining Handayani Nining Handhayani Noer Muhammad Dliyaul Haq Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Noor RR Nurhayati, Retno Wahyu Nurhidayat - Nurhidayat Nurhidayat Nuril Farizah Nursanti, Risa Nuzulia, Nur Aisyah Prakoso, Nurul Muhammad Prasetyaningtyas, Wahono Puspitasari, Riris R I Arifiantini R Sumarmin, R Rachmat Herman Rahmaniyah, Wiwit Ridhani Rahmat Hidayat Rahminiwati, Min Ramadhan Sumarmin Rangga Setiawan Rangga Setiawan Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Resti Rahma Dianti Ridi Arif Rimayanti - Rini Widyastuti Riris L. Puspitasari Riris L. Puspitasari Rizal M Ronny Rachman Noor Salsabila, Cyntia Bella Sandy Qlintang SATRIYAS ILYAS Satya Gunawan Seiichi Watanabe Shofwal Widad Sigit Prastowo Siti Darodjah Rasad Sony H. Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO SONY HERU SUMARSONO Sri Catur Setyawatiningsih Srihadi Agungpriyono Subangkit, Mawar Sulistiono Sumantri C Sumarsono, Sony H. Sumarsono, Sony Heru Sundari, Ayu Mulia Supar - Supar . Sutarya Enus Sutiman Bambang Sumitro T adelina T R Wiradarya T Toharmat T. Suzuki T. Toharmat T. Toharmat TAKDIR SAILI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Jamaan Thomas Mata Hine TL Yusuf, TL Trevino A. Pakasi Tri Aprilliana Wulandari TRINIL SUSILOWATI Tutik Wrediati Tutik Wresdiyati Tutty Laswardi Yusuf Tutty Laswardi Yusuf Tuty Laswardi Yusuf Uswatun Hasanah Vincentia Maria Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin Wasmen Manalu Widjiati Widjiati, Widjiati Wildan Mubarok Wining Astini Wining Astini Wiwit Ridhani Rahmaniyah Y Yulnawati Yessie Widya Sari Yoga Yuniadi Yuhara Sukra Yuhara Sukra Yulnawati . Yulnawati Y YULNAWATI YULNAWATI Yundari, Yundari Yushinta Fujaya

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