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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Ilmu dan Teknologi Kelautan Tropis Hemera Zoa Media Veteriner Majalah Ilmiah Peternakan Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Reproduksi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology ZOO INDONESIA Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal ARSHI Veterinary Letters Jurnal Kedokteran Hewan Current Biomedicine

Arief Boediono

Bogor Agricultural University

Author-ID : 232094

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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PCS-16 Determaining Surgical Method by Meniscectomy Induction on Garut sheep (Ovis aries) for early stage of Osteoarthritis Handina Rakhmawati; Adrian Situmeang; . Nurhidayat; Andri Maruli Tua Lubis; Harry Murti; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Osteoarthritis (OA) is the most common joint disease that cause of pain and disability by various factors such as advanced age, obesity, trauma, and arthritis disease. These factors affect by degeneration of the cartilage surface, leading to loss of matrix include proteoglycan osteophyte formation, subcondral and synovial membrane affected. In the healthy joint, meniscus, articular cartilage, subchondral bone, and synovial membrane provide support to the joint. The meniscus is an important load bearing structure and has nutritive as well as lubricating properties in the knee joint as well (Little et al. 2010).Animal models are research materials that can be used in studying potential pathogenesis and therapy in various diseases in humans. Sheep are commonly large animal model of OA because of the availability, ease of handling, and have a similarities with humans in size and structure of joint. In the development of science, sheep can be used as an animal model in studying the pathogenesis of diseases in human orthopedics studies such as joints, ligaments, and bones. Garut sheep is an Indonesian germplasm indigenous that has the structure, density, and size of joint anatomy that are similar in human joints rather than other small animals. This is the basis of the utilization of Garut sheep as an animal model in human orthopaedic. (Little et al. 2010; Gregory et al. 2012).The aim of this study was to identify and analyze the determining surgical method by meniscectomy induction on Garut sheep with 8 weeks post meniscectomy observation for early stage of OA.

In Vitro Development Of Holstein And Japanese Black Breeds Embryo A. Boediono; T. Suzuki
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Two experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulatedcow serum (SCS) and fetal calf serum (FCS) supplementation into medium for in vjtro development (experiment I) and the effect of different source of oocytes (experiment II). Theendpoint was development to cleavage on day 2 and to blastocyst up to day 9 after msermnabon. In experiment I, oocytes were matured and cultured m vltro (after fertilization)in TCM-199 supplemented with 5% SCS or 5% FCS. The cleavage rate of zygotes cultured in medium supplemented with 5% SCS was higher (P

Morphology and Histomorphometry of Testis And Epididymis of Kacang Goat (Capra sp.) and Local Sheep (Ovis sp.) Citra Noviana; Arief Boediono; Tutik Wresdiyati
Media Veteriner Vol. 7 No. 2 (2000): Media Veteriner
Publisher : Media Veteriner

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Penelitian ini bertujuan untuk mempelajari morfologi dan histomorfometri testis dan epididymis kambing kacang (Capra sp.) dan domba lokal (Ovis sp.) dengan umur 1-1,setahun. Secara makroskopik, keliling, berat dan volume testis kambing kacang lebih kecil (P

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Sperm motility is the main problem for honeybee's reproduction both in natural and in artificial insemination. Sperm produced only by sexually mature drone. Drone with high semen volumes can be seen by the external morphology like hairy in back and the existence of yellow stripes on their black abdomen. Semen samples were collected from the drone by the massage technique; these consist of massaging the abdomen of drone. This massage proceeded until penis and semen cream to yellow color, was produced. Semen taken out with spuit (3 ml) that contain a modification dilution media called "Kiev". Semen samples were collected; volume, abnormalities and motility were measured. The sexually mature drones were observed in 99.06% (213/215) drones with rare hair in back and 76.28% (164/215) with existence of the yellow stripes in the black abdomen. The color of sperm is yellowish white, which is different from mucous that is white. The average volume of semen samples from each drone is 1.11 μl. The average length of sperm is 217.57 μm (107.50-412.50 μm). The average length of sperm is 7,52 μm (5-1O μm). It's flipped tails, broken tails, double tails, and double heads with an average of 19.83%, 12.75%, 6.42%, and 2,25% respectively can determine abnormalities of the sperm. To conduct the artificial insemination, drone sperm should always be available. For this reason sperm should be preserved in an optimal condition storage temperature (5°C, 27 °c and 37°C) and glucose concentration (0%, 0,3%, 0,6%, and 0,9%) containing in the dilution media. This condition can be affecting the sperm motility. The highest motility is achieved when the sperm is kept in 0,9% glucose concentration and storage in 5 °C temperature. In this condition 0.19x106 sperm/ml can survive up to 36 hours. Moreover, the higher concentration of sperm motility up to 36 hours is achieved when sperm is kept in 5 Celsius temperature with different leves of glucose in dilution media. However statistically interaction between glucose concentration and temperature levels does not give significant affects.

SL-07 Competency of Diploid Parthenogenesis Embryonic Stem Cell (pESC) for Cell Therapy Arief Boediono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Naturally, mammalian embryo resulting from the fertilized oocytes by sperm and develops to the zygote with diploid chromosome. Through the embryo engineering, mammalian embryo also could be produce by: nuclear transfer and parthenogenesis. Embryo produce by nuclear transfer method could develop to term with very limited results. However, parthenogenetic embryos could not develop to term because of the incorrect expression of imprinted genes (genomic imprinting).........

Microscopic Analysis of The Lung in Male Rat (Rattus Norvegicus) Exposed to Cigarettes Smoke Adrien Jems Akiles Unitly; Nastiti Kusumorini; Srihadi Agungpriyono; Aryani Sismin Satyaningtijas; Arief Boediono
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 10, No 2 (2018): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

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This research aimed to study on the microscopic changes of lungs in male rat that exposured to cigarette smoke. A factorial CRD with periode of treatment and sample collection was applied in this study. An exposure of cigarette smoke was carried out at 10 cigarettes/rat/day for 2.5 hours in an smoking chamber. Group N is untreated animals. Group 20d is exposed animals with cigarette smoke for 20 days consectitively and released from the cigarette smoke exposure for 20 days. Group 40d is exposed animals for 40 days and released from the cigarette smoke for 40 days. Group 60d is exposed animals for 60 days similar as above. Data collection was carried out twice : after exposure and after redeasing the cigarette smoke exposure. The parameters of observation microscopic analysis of lungs included weight wet of lung and change of lung histopathologic.The results show decreased weight wet of lung, increased number of blackish particulate deposits in the cytoplasm of alveoli at 40d and 60d considered as tar deposit, an increased activity of inflammatory cells of male rats after exposing which were unable to recover after releasing of cigarette smoke exposure.

Status of ram spermatozoa DNA after freeze-drying process Takdir Saili; wahono Esthi Prasetyaningtyas; Mohamad Agus Setiadi; Srihadi AgungPriyono; Arief Boediono
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay

Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Rahmat Hidayat; T. Toharmat; A. Boediono; I.G. Permana
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatments and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly. The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD. DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams

Epididymal sperm quality of buffelous with Different Spotted Types Yulnawati Y; Rizal M; Maheshwari H; Noor RR; Sumantri C; Boediono A
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The significant decline of spotted buffalo population nowadays brought this species into an endangered situation. To perform an integrated conservation project, we need some basic information and data related to the reproductive and genetics potency of this buffalo. The purpose of this study was to observe the effect of coat color variation to the sperm quality, in order to get focus on specific candidate gene that allegedly bring the causative mutation(s) and responsible for the different pigmentation expression. In this study, we compare the quality of fresh and frozen-thawed epididymal sperm from 12 spotted bulls (that classified in 3 different spotted types, i.e Saleko, Bonga, and Lotong Boko) with five solid bulls. The results showed that there were no significant differences (P > 0.05) in all parameters of fresh and frozen-thawed epididymal sperm among those groups. The percentage of frozen-thawed progressive motility from Saleko, Bonga, Lotong Boko, and Solid was 44%, 42%, 40% and 42%, respectively. Moreover, the percentage of livability and membrane integrity of frozen-thawed sperm from each groups were 64.9%; 65.2%; 62.6%; 62.7% and 64.6%; 67.1%; 64.5%; 64.1%. In conclusion, it suggested that the coat color/phenotype difference has no effects on the quality of fresh and frozen-thawed epididymal sperm of spotted buffalo. Key Words: Epididymal Sperm, Spotted Buffalo

Role of various sugars in improving frozen semen quality of Garut ram Muhammad Rizal; Herdis .; Arief Boediono; Achmad Selamet Aku; Yulnawati .
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Ram spermatozoa are sensitive to extreme changes in temperature during the freeze-thawed process. The present study was conducted to examine the effects of addition of various sugars in Tris extender on sperm cryosurvival of Garut ram. Semen was collected using an artificial vagina from three mature rams once a week. Immediately after initial evaluation, semen was divided into five parts and diluted with Tris extender (control), Tris extender + 0.4% dextrose, Tris extender + 0.4% raffinose, Tris extender + 0.4% trehalose, and Tris extender + 0.4% sucrose, respectively. Semen was loaded in to 0.25 ml mini straw with the concentration of 200 million or 800 million motile spermatozoa per ml. Semen was equilibrated at 5oC for three hours, then frozen and stored in liquid nitrogen for seven days. Quality of processed-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), intact acrosome cap (IAC), and intact plasma membrane (IPM) were evaluated after dilution, equilibration, and thawing, respectively. Data were analyzed using completely randomized design with five treatments and six replicates. Means were compared significant difference test at 0.05 significant level. Results of this research showed that there was no significantly difference (P>0.05) between treatments for all sperm quality parameters after dilution and equilibration. Mean percentages of post thawing MS, LS, IAC, and IPM for dextrose (54.00; 68.00; 66.60, and 57.83%), raffinose (50.00; 64.33; 61.80, and 61.75%), trehalose (50.83; 65.67; 61.40 and 57.75%), and sucrose (49.00; 66.80; 58.50 and 58.50%) were significantly (P<0.05) higher than control (40.83; 52.67; 54.60, and 49.40%) respectively. In conclusion, addition of 0.4% dextrose, raffinose, trehalose or sucrose in Tris extender are effective in improving frozen semen quality of Garut ram. Key Words: Sugars, Tris extender, Frozen Semen Quality, Garut Ram

Co-Authors . AULANI’AM . Herdis . Yulnawati A Winarto A.S. Satyaningtijas Abinawanto Adi Winarto Adisti Dwijayanti Adkhilni Utami Adkhilni Utami Adrian Situmeang Adrian Situmeang Adrian Situmeang Adrien Jems Akiles Unitly Adrien Jems Akiles Unitly, Adrien Agus Harsoyo Agus Harsoyo Agus Oman Sudrajat Agus Setiadi Ahmed, Ifty Al Azhar Al Mukhlas Fikri AL-AZHAR AL-AZHAR Alfred O. M. Dima Alif Iman Fitrianto Alif Iman Fitrianto Alimuddin Alkaustariyah Lubis Amrozi Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andri Maruli Tua Lubis Andri Maruli Tua Lubis Andriyanto A Andriyanto Andriyanto ANOM BOWOLAKSONO Arie Adrianus Polim AS Aku, AS Aucky Hinting Aulia Miftakhur Rahman Ayu Mulia Sundari Bambang Kiranadi Batara Sirait Bayu Rosadi Berry Juliandi Bibiana W Lay BIBIANA W LAY Boenjamin Setiawan Boenjamin Setiawan Budiariati, Vista Cahayadi, Sigit Daru Cece Sumantri Chairun Nisa Citra Noviana Cutnya’ Shaliran Nazlie (Alm) D Sudrajat Dedy D. Solihin Diah Nugrahani Pristihadi Dian Anggraini Djaswadi Dasuki Djoko Walujo Dody Dharmawan Trijuno Dondin Sajuthi Dwi Budiono Dwierra Evvyernie Dwiranti, Astari Elpita Tarigan Eni Kusrini EVY AYU ARIDA F Amir F Fathul Farid A. Moeloek Ferry Sandra Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Funahashi, Hiroaki H Maheshwari Hadi, Restu S. Handina Rakhmawati Handina Rakhmawati Harry Murti Harry Murti Harry Murti Harry Murti Hartanti Kusumaningtyas HERA MAHESHWARI Herdis . herdis herdis Heri Sujoko Heru Setijanto I G Permana I Ketut Mudite Adnyana I Ketut Suatha I Supriatna I Wayan Batan I.G. Permana Ichsan Ichsan Iis Diatin Iman Supriatna Indra Bachtiar Indra Kusuma Irma H Suparto Irma Herawati Suparto Irma Suryani Ita Djuwita Ita Djuwita ITA DJUWITA Ita Fauzia Hanoum, Ita Fauzia Ivan Sini Karisma Mardatillah Karisma Mardatillah Kartini Eriani KARTINI ERIANI KARTINI ERIANI Kartiwa, R. Angga Kelvin Yaprianto Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Krido Brahmo Putro Kusdiantoro Mohamad Kusumaningtyas, Hartanti Latifah Kosim Darusman Lea Tarliyah Lindiawati, Riris M Agus Setiadi M Fakhrudin M Imron M Rizal M. Haviz M.A Setiadi Madihah Madihah Maheshwari H Maman Surachman Mas Rizky A.A. Syamsunarno Maula, Yogi Nikmatul Miraprahesti, Retti N. Mohamad Fakhrudin Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Gunawan Muhammad Gunawan Muhammad Gunawan MUHAMMAD RIZAL Muhammad Rizal Muhammad Rizal Muhammad Rosyid Ridlo Muhammad Zairin JR Muhammad Zairin Jr. MULYOTO PANGESTU MULYOTO PANGESTU Muslim Muslim N M D Haq Nastiti Kusumorini Nastiti Kusumorini NASTITI KUSUMORINI Nining Handayani Nining Handayani Nining Handhayani Noer Muhammad Dliyaul Haq Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Noor RR Nurhayati, Retno Wahyu Nurhidayat - Nurhidayat Nurhidayat Nuril Farizah Nursanti, Risa Nuzulia, Nur Aisyah Prakoso, Nurul Muhammad Prasetyaningtyas, Wahono Puspitasari, Riris R I Arifiantini R Sumarmin, R Rachmat Herman Rahmaniyah, Wiwit Ridhani Rahmat Hidayat Rahminiwati, Min Ramadhan Sumarmin Rangga Setiawan Rangga Setiawan Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Resti Rahma Dianti Ridi Arif Rimayanti - Rini Widyastuti Riris L. Puspitasari Riris L. Puspitasari Rizal M Ronny Rachman Noor Salsabila, Cyntia Bella Sandy Qlintang SATRIYAS ILYAS Satya Gunawan Seiichi Watanabe Shofwal Widad Sigit Prastowo Siti Darodjah Rasad Sony H. Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO SONY HERU SUMARSONO Sri Catur Setyawatiningsih Srihadi Agungpriyono Subangkit, Mawar Sulistiono Sumantri C Sumarsono, Sony H. Sumarsono, Sony Heru Sundari, Ayu Mulia Supar - Supar . Sutarya Enus Sutiman Bambang Sumitro T adelina T R Wiradarya T Toharmat T. Suzuki T. Toharmat T. Toharmat TAKDIR SAILI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Jamaan Thomas Mata Hine TL Yusuf, TL Trevino A. Pakasi Tri Aprilliana Wulandari TRINIL SUSILOWATI Tutik Wrediati Tutik Wresdiyati Tutty Laswardi Yusuf Tutty Laswardi Yusuf Tuty Laswardi Yusuf Uswatun Hasanah Vincentia Maria Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin Wasmen Manalu Widjiati Widjiati, Widjiati Wildan Mubarok Wining Astini Wining Astini Wiwit Ridhani Rahmaniyah Y Yulnawati Yessie Widya Sari Yoga Yuniadi Yuhara Sukra Yuhara Sukra Yulnawati . Yulnawati Y YULNAWATI YULNAWATI Yundari, Yundari Yushinta Fujaya

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