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Keberhasilan IB menggunakan semen beku hasil sexing dengan metode sentrifugasi gradien densitas percoll (SGDP) pada sapi Peranakan Ongole (PO) Moh Thoriq Fernanda; Trinil Susilawati; Nurul Isnaini
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 24, No 3 (2014)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study was to evaluate the implementation of Artificial Insemination using sexed frozen semen processed with percol density gradient sentrifugation method on service per conception (S/C), Non Return Rate (NRR) and Conception Rate (CR) of Ongole crossbred cattle. The material used in this study were 54 Ongole crossbred cattle which were purposively selected with criteria had conditions of estrus 2A+. Fifty five cattle were divided into 2 treatments such as P0 (non sexed semen) with Post Thawing Motility (PTM) quality 40% and P1 (sexed semen) with PTM quality 5-10%. The study was conducted in the village Boro Bunut and Boro Kemantren Pakis subdistrict Malang. The study showed that P0 (non sexed semen) had S/C 1.3, NRR0-21 74.07%, NRR22-42 74.07%, NRR43-63 74.07% and CR 74.07%, while P1 (sexed semen) had S/C 1.5, NRR0-21 74.07%, NRR22-42 59.25%, NRR43-63 59.25% and CR 55.56%. These results indicated that P0 (non-sexed semen) had better results than P1 (sexed semen), but statistically the two treatments were not significantly different (P>0.05). The highest percentage of cattle pregnancy (74.07%) was found in P0 (non sexed semen) as compared to P1 (sexed semen) which was 59.25%.   Keywords: Sexing, estrus, cattle
Pengaruh lama gliserolisasi terhadap keberhasilan produksi semen beku Sapi Simmental Sudarmanto Sudarmanto; Trinil Susilawati; Nurul Isnaini
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 25, No 2 (2015)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2015.025.02.06

Abstract

The purpose of this research was to determine duration effect of different glicerolitation time on the quality and frozen semen production of Simmental bulls in Ungaran Artificial Insemination Center. The materials of the research used four Simmental bulls. The method of the study used experimental laboratory. There were two treatments in the study (P0 = duration of glicerolitation for > 3 hours, P1 = duration of gliserolitation for 15 minutes) and four Simmental bulls. The research parameter included semen volume, pH, concentration of spermatozoa, frozen semen production and post thawing motility from 8 treatments. Data were analyzed using t-test. The quality of fresh semen had average percentage of motility 70% and 2+. The research showed that the frozen semen production during 15 minutes of glicerolitation was better than that of >3 hour duration. Moreover, the motile sperms produced during 15 minutes of glicerolitation was higher than those which produced during >3 hours of glicerolitation. Keywords: motility, glicerolitation, frozen semen
Keberhasilan inseminasi buatan menggunakan semen beku dan semen cair pada sapi Peranakan Ongole Trinil Susilawati; Nurul Isnaini; Aulia Puspita Anugra Yekti; Ika Nurjannah; Errico Errico; Nolasco da costa
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 26, No 3 (2016)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2016.026.03.03

Abstract

The purpose of this research was to know about the successfully artificial insemination (AI) using frozen and liquid semen of Ongole cross (PO) cattle indicated by Service per Conception (S/C), Days Open (DO) dan Conception Rate (CR). The materials used were 60 heads of Ongole crossbred cattle which were selected purposively using some criteria, such as having calving experience, healthy, and free from any reproduction disorder. This field experiment consisted of direct observation and interview to acquire primary and secondary data. Datas were analyzed descriptively and continued by a paired t-test. The results showed that the value of (S/C) was very significantly different (P<0.01),  DO was not significantly different (P>0.05). CR for P1, P2, P3, and P4 was found 63.33% , 86.67%, 16.33%, 83.33% respectively. The conclusion of this research was AI using frozen and liquid semen with only 5 days preservation had a difference on the value of service per conception. The most successfull AI was found higher in the liquid semen which was stored for 1 and 5 days than that of frozen semen. Key words : semen, service per conception, days open, calving interval
The effect of age on semen freezability of Swamp Buffalo bull in a commercial artificial insemination center Nurul Isnaini; Tri Harsi; Wahyu Riza Zamani
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 3 (2019): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.03.09

Abstract

This study aimed to assess the effect of age on semen freezability (sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate) of swamp buffalo bull kept in Lembang Artificial Insemination Center. A total of 115 ejaculates collected from one swamp buffalo bull at 5, 6 and 7 years old were included in the study. The results showed that the overall mean values of sperms motility before freezing, post-thawing, recovery rate, and straws production per ejaculate of swamp buffalo bull were 62.13%, 43.61%, 62.16%, and 114.16 doses, respectively. The age of bull did not show significant (P>0.05) effect on sperms motility before freezing, however the sperms motility post-thawing, recovery rate, and straws production per ejaculate was influenced (P<0.01) by bull age. At seven years old, the poorest (P<0.01) post-thawing sperms motility and recovery rate were noted in comparison to 5 and 6 years old, nevertheless the difference in post-thawing sperms motility was only less than 3% point, which was not biologically important. On the other hand, the semen collection at seven years old had a higher (P<0.01) straws production per ejaculate than those at 5 and 6 years old (133.70 vs. 106.39 and 90.61 doses, respectively). In conclusion, this study provides evidence that the semen freezability of swamp buffalo bull is varied among the age with the peak frozen semen production was achieved at seven years old.
Kualitas semen segar dan recovery rate sapi bali pada musim yang berbeda Siti Aisah; Nurul Isnaini; Sri Wahyuningsih
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 27, No 1 (2017): April
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2017.027.01.06

Abstract

This research was conducted at the Center  of Artificial Insemination (BBIB)Singosari , Malang, East Java. The purpose of this research was to determinethe parameters of fresh semen quality including volume, pH, individual motilityand concentration of frozen semen production and  recovery rate of  Bali bullsin different seasons. The material used were fresh semen of 14 heads of Bali’sbull in 3, 5, 6, 10, and 11 years and secondary data from November 2015 toSeptember 2016 at BBIB Singosari. The method used in this research was casestudy and the data were analyzed descriptively. The results showed that thehighest volume semen Bali bull in summer was 6.05±1.50 ml. The pH in rainyseason and summer were 6.5±0.07. Semen of Bali’s bull dominated by the white-milk color. The highest of color (white-milk), individual motility, concentrationand total of frozen semen production were at summer in September. The average ofcolor, individual  motility, concentration  and total of fozen semen productionwere 89%±0.10, 66.98 ± 0.05%, 1159.25 ± 211.28 million/ml and 22682 ± 707.95straw. Post thawing Motility (PTM) and RR value were 45.28 ± 0.02%; 66.79± 0.05semen of Bali’s bull highest at summer in August. The conclusion  showed thedifferences of season will affect to volume, color, individual motility,concentration, PTM, the value of RR and production of frozen semen Bali bull,but it is not effect for pH semen.
PENGGANTIAN BOVINE SERUM ALBUMIN PADA CEP-2 DENGAN SERUM DARAH SAPI TERHADAP KUALITAS SEMEN SAPI LIMOUSIN PADA SUHU PENYIMPANAN 3-5oC Feri Eka Wahyudi; Trinil Susilawati; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 2 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.939 KB) | DOI: 10.21776/ub.jtapro.2016.017.02.2

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggantian Bovine Serum Albumin (BSA) menggunakan serum darah sapi pada pengencer dasar Cauda Epididymal Plasma 2 (CEP-2) terhadap kualitas semen sapi Limousin selama pendinginan pada suhu 3-5oC. Materi penelitian yang digunakan yaitu semen segar dari sapi Limousin yang dipelihara di Balai Besar Inseminasi Buatan Singosari Malang. Serum darah didapatkan dari darah sapi yang diambil dari Laboratorium Lapang Sumber Sekar Fakultas Peternakan Universitas Brawijaya. Metode yang digunakan dalam penelitian menggunakan percobaan laboratorium dengan Rancangan Acak Kelompok yang terdiri dari lima perlakuan, yaitu P0 sebagai kontrol (90% CEP-2 (dengan BSA) + 10% KT); P1 (83,84% CEP-2 + 6,16% serum + 10% KT); P2 (81,84% CEP-2 + 8,16% serum + 10% KT); P3 (79,84% CEP-2 + 10,16% serum + 10% KT) dan P4 (90% CEP-2 (tanpa BSA) + 10% KT). Parameter yang diamati adalah motilitas, viabilitas dan abnormalitas. Hasil penelitian, persentase motilitas yang tertinggi yaitu P0 (37,6±17,3%). Persentase viabilitas yang tertinggi yaitu P3 (63,4±3,5). Persentase abnormalitas yang tertinggi yaitu P0 (16,4±4,0%). Total spermatozoa motil pada semua perlakuan lebih besar daripada kriteria Standar Nasional Indonesia (SNI) yaitu 40% spermatozoa motil dari total konsentrasi. Disimpulkan bahwa serum darah belum mampu menggantikan BSA pada CEP-2, namun pada pengencer dengan serum 6,16% cukup mampu mempertahankan motilitas spermatozoa walaupun tidak sebaik pengencer dengan BSA. Kata kunci : semen cair, penyimpanan dingin, motilitas, viabilitas, abnormalitas
PENGARUH SUPLEMENTASI FILTRAT KECAMBAH KACANG HIJAU (Phaseolus radiatus L.) TERHADAP KUALITAS SEMEN SAPI SIMMENTAL DALAM PENGENCER SKIM MILK PADA SUHU DINGIN Ayu Melia Sades; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.169 KB) | DOI: 10.21776/ub.jtapro.2016.017.01.1

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi filtrat kecambah kacang hijau dalam pengencer skim milk terhadap kualitas semen sapi Simmental pada suhu dingin. Materi penelitian yang digunakan adalah semen pejantan sapi Simmental dengan kriteria umur 5-7 tahun dan memiliki motilitas individu ≥50%. Metode penelitian yang digunakan adalah pola Rancangan Acak Kelompok (RAK) dengan 4 perlakuan dan 6 kali ulangan. Semen diencerkan dalam pengencer skim milk dengan 4 perlakuan (0%, 2%, 4% dan 6% filtrat kecambah kacang hijau). Hasil penelitian menunjukkan bahwa penambahan filtrat kecambah kacang hijau ke dalam pengencer skim milk memberikan perbedaan yang tidak nyata (P>0,05) terhadap motilitas, viabilitas dan abnormalitas spermatozoa. Kesimpulan dari penelitian ini adalah penambahan filtrat kecambah kacang hijau dalam pengencer skim milk tidak dapat mempertahankan kualitas semen cair (motilitas, viabilitas dan abnormalitas) sapi Simmental yang disimpan pada suhu dingin. Saran untuk penelitian selanjutnya adalah perlu studi lebih lanjut mengenai pengaruh suplementasi filtrat kecambah kacang hijau dalam pengencer skim milk terhadap kualitas semen cair sapi Simmental dengan menggunakan semen segar yang memiliki motilitas awal sesuai standart yaitu ≥70%.Kata kunci: kualitas spermatozoa, semen cair, penyimpanan suhu dingin.
TAMPILAN REPRODUKSI HASIL INSEMINASI BUATAN MENGGUNAKAN SEMEN BEKU HASIL SEXING PADA SAPI PERSILANGAN ONGOLE DI PETERNAKAN RAKYAT Lieyo Wahyudi; Trinil Susilawati; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.415 KB)

Abstract

The purpose of this research was to determine the success of AI using sexed semen results based on NRR, S/C, CR, and rectal palpation were also determine.  The total number of 81 acceptors divided into 3 treatment groups, such as P0 (non- sexed semen) ; P1 (Frozen semen Sexed using White Yolk Sedimentation),  P2 (Frozen semen sexed using Percoll Gradient Centrifugation) . The results showed the NRR percentage of P0, P1 and P2 were between the treatments are P0 of 74.07 ± 12:00%; 65.43 ± 5.66% and 64.19 ±  8:56% respectively. Furthermore the highest of CR was in P0 44%,   P2 25.91% and P1 18.51%. Conclution of this research was the highest percentage of pregnancy was 59.25 %(P0) ,  P1 51.85% and the lowest was in 44.44%. The S/C was in (P0) 2.31 follow by 3.00 in P2 and 3.33 in P1.   Key words : artificial insemination , frozen semen sexed, the percentage of Gestation and feed
PENGARUH PENGGUNAAN PENGENCER SKIM MILK DENGAN BERBAGAI LEVEL FILTRAT KECAMBAH KACANG HIJAU (Phaseolus radiates L.) TERHADAP KUALITAS SEMEN CAIR SAPI SIMMENTAL PADA SUHU RUANG Inna Yatusholikhah; Nurul Isnaini; Muhammad Nur Ihsan
TERNAK TROPIKA Journal of Tropical Animal Production Vol 16, No 2 (2015): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.374 KB) | DOI: 10.21776/ub.jtapro.2015.016.02.3

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan pengencer susu skim  dengan berbagai level filtrat kecambah kacang hijau (Phaseolus radiatus L.) terhadap kualitas semen cair yaitu motilitas individu, viabilitas dan abnormalitas spermatozoa sapi Simmental yang di simpan papda suhu ruang. Manfaat pada penelitian menjadi studi ilmiah sebagai sumber daya untuk akademisi dan untuk pelaksanaan teknis IB. Materi penelitian yang digunakan yaitu semen segar dari sapi Simmental yang dipelihara di Balai Besar Inseminasi Buatan Singosari Malang, umur 5-7 tahun, motilitas massa ++ dan motilitas individu ≥ 50%.. Metode yang digunakan dalam penelitian menggunakan percobaan laboratorium dengan Rancangan Acak Kelompok yang terdiri dari empat perlakuan, yaitu P0 sebagai kontrol (1 ml susu skim + 0,1 semen), P1 (0,02 ml filtrat (Phaseolus radiatus L.) + 0,98 ml susu skim + 0,1 semen), P2 (0,04 ml filtrat (Phaseolus radiatus L.) + 0,96 ml susu skim + 0,1 semen) Dan P3 (0,06 ml filtrat (Phaseolus radiatus L.) + 0.94 ml susu skim + 0,1 semen). Data dianalisis dengan menggunakan ANOVA dalam Rancangan Acak, adanya pengaruh yang signifikan akan diuji oleh Uji Jarak Berganda Duncan. Hasil penelitian menunjukkan bahwa motilitas individu dan abnormalitas spermatozoa tidak berbeda nyata (P> 0,05) untuk semua penyimpanan pada 0, 2, 4 dan 6 jam, sedangkan viabilitas spermatozoa memberikan perbedaan yang nyata (P <0,05) untuk P3 (69,34 ± 11,01) pada penyimpanan 4 jam. Kesimpulan dari penelitian ini adalah pengaruh pengencer susu skim dengan berbagai level filtrat kecambah kacang hijau terhadap kualitas semen cair sapi Simmental pada suhu ruang hanya memberikan pengaruh pada vaibilitas spermatozoa dengan level 4% yang di simpan pada 4 jam. Kata Kunci : semen cair, motilitas, viabilitas, abnormalitas
PENGARUH PENGHILANGAN RAFINOSA DALAM PENGENCER TRIS AMINOMETHANE KUNING TELUR TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA SIMPAN DINGIN Abdul Rochim; Muhammad Ade Salim; Nurul Isnaini; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.049 KB) | DOI: 10.21776/ub.jtapro.2017.018.01.5

Abstract

The objective of this research was to examine the effect of removal raffinose in tris aminomethane egg yolk diluents to semen quality of Boer goat during chilled preservation. This research was carried out at Animal Reproduction Laboratory and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University from December 11th 2016 to  January 31th 2017. Semen diluent was divided into two groups, there were P0 (80% Tris Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose) + 20% Egg Yolk). Parameter of this research was motility percentage, viability percentage, and abnormality percentage. Data of this research was analyzed using paired design t test. The result showed that after five days chilled preservation, was different (P<0,05) on the average motility percentage between P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different (P<0,01) on the average viability percentage between P0 (45,86±15,66%) and P1 (46,38±15,32%). There was significantly different (P<0,01) on the average abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total motile sperm count after 5 days chilled preservation was different (P<0,05) in P0 treatment and not different (P>0,05) in P1 treatment compared to the expectation value of 40 million sperm/ml. The conclution of this research was the tris aminomethane egg yolk diluent without raffinose could protect of Boer goat semen quality during chilled preservation. Keywords: boer goat semen, tris aminomethane, raffinose, chilled preservation
Co-Authors A.S, Veronica Dyah Abdul Rochim Ade Tiara Adelina Ari Hamiyanti Adnan Reza, Faisal Akhmad Kurniawan Alfa Fajarrofa Alfana, Ratu Ali Mahfud Amran, Rovita Andi Thahir Anggit Damaratri Lapoliwa Anggraini Anggraini Annisa Nyuwita Ardyah Ramadhina Irsanti Putri Ariesty, Ghina Ayu Arif, Ani Atul Arrahman, Rudi Arrow Sakti Darren Leksono Artharini Irsyammawati Artharini Irsyammawati Asri Nurul Huda Astuti, Ni Made Marnita Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Aviliani, Dita Awang Tri Satria Ayssa, Gusti Al Ayu Melia Sades Ayu Sulvi Istanty Aziz, Achmad Fadhli Bastianus Doddy Riyadi Budjana, Diajeng Wulandari Cahyana, Dian carmidah carmidah Choirun Ni'mah Choirun Nisak Darsono, Susilo Nur Aji Cokro Debrina Chandra Wiyanti Dedes Amertaningtyas Dedi Muhammad Devi Maharani Dewi Yuliasari Dian Cahyana Durinda Puspasari Eliwarti Eliwarti, Eliwarti Elpiana Elpiana Ermasari, Anissa Eros Sukmawati Errico Errico Ervina Martiana Evayanti, Yulistiana Evrianasari, Nita Faisal Adnan Reza Faizal Andri Fajar Amaliah Fatmah, Maryani Fazrien, Wahyu Alif Febrianto, Nanang Feri Eka Wahyudi Feri Eka Wahyudi Firman, Afrizal Fitria, Puput Gagas Erdi Saputro Genovicha, Bendsia Hadisuyitno, Juin Hanief Eko Sulistyo Hanief Eko Sulistyo Hartandi, Nugroho Hermanto Herwijanti, Enniek Ika Fitri Puspitasari Ika Nurjannah Inna Anggraeni Inna Yatusholikhah Intan Islamia Islamy, Chairunnisa Putri Ismail Hasan, Ismail iswati iswati Iswati Iswati Junaedi Junaedi Junaedi Junaedi Juniandri . Komalyna, I Nengah Tanu Kurniaesa, Titah Ulya Kurniawati Kurniawati Kusumawati, Enik Dwi Kuswati Kuswati Kuswati Kuswati Lantarno Lely Ika Mariyati Leondro, Henny Lieyo Wahyudi Lukman Affandhy Sunarto M Ade Salim M Fajar Agustian M Nur Ihsan M Nur Ihsan M. Adhyatma Maghfiroh, Noni Ashri Marcella, Fanna Mardiana, Riril Mariza, Ana Mashudi Mashudi Maternity, Dainty Meilia Syafitri Miftakhul Jannah Mira Agustina Moch Saifudin Mochammad Saifur Moh Nur Ihsan Moh Thoriq Fernanda Moh. Miftahul Falah Mohammad Efendi Mokhamad To'ib Muchamad Lutfhi Muchamad Luthfi Mugiyati Mugiyati Muhammad Ade Salim Muhammad Halim Natsir Muhammad Halim Natsir Muhammad Hatta Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Nur Ihsan, Muhammad Nur Muhammad Sabri Muhammad Yayang Eka Kurniawan Murcahyana Murcahyana Nafis, Fardha Ad Durrun Naida, Firgiani Puspita Neneng Siti Latifah Ningsih, Astri Cahya Nisa Umrotul Mila Nisa’us Sholikah Nolasco da costa Nugraha, Pazri Nurfaqillah, Nurfaqillah Nurhasanah Nurhasanah Nurul Huda Nuryadi Nuryadi Pawestri, Hartini Prasetyaning Pradana, Satria Adi Pratiwi Trisunuwati Purnaningsih, Widarti Tri Puspita, Gita Anggara Putri Utami, Putri Rahayu Lestari Rahman Maulana Rahmawati, Nanda Ayu Rama Diyanti Ramadhan, Bintang Djodi Rara Putri Audia Renfiana, Lilis Revangga, Yanda Rico Reza Refiani Rina Novita Sari Indah Rizky Maulana, Rizky Rohayati, Lilis Rosi Novita sari Rosmiyati Rosmiyati Rotua Aruan, Rumiri Rumiri Rotua Aruan, Rumiri Rotua rusadi, paramita Rusno Rusno Samino Samino Sari, Fenny Refika Sari, Nita Evriana Septania, Sovi Septianingsih, Triana Siti Aisah Siti Sunami Sitiadira Aulia Aldini Soviska Nur Aini Sri Minarti Sri Wahjuningsih Sri Wahyuningsih Sudarmanto Sudarmanto Suhandi Suhandi, Suhandi Sulistyo, Hanief Eko Sumardi, Lalu supriadin supriadin Syah, Habib Asshidiq Syamsul Bahri Tibar, Sri Linda Azzahra Tomy Suganda Tri Agus Sulistya Tri Harsi Tri Harsi Trinil Susilawati Trinil Susilawati Try Puji Lestari Umam, Fatihul Utami, Vida Wira Wahjuningsih , Sri Wahyu Riza Zamani Wahyudi, Aditiya Waras Harianto Wenas, Karl Wihatyani, Ketut Yahya, Fizian Yuli Yantina Yulia Indri Anastasia Yulistiana eva yanti Zenni Nur Fadilah