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Maskur Maskur
STMIK AMIKOM Purwokerto
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White Blood Cell Differentiation Based on Toll-Like Receptor 4 (TLR4) Gene Polymorphism in Local Indonesian Chickens Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, L.; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6518

Abstract

Toll-like Receptors (TLRs) genes are a group of genes that transcribe TLRs proteins which act as receptors on the surface of phagocytic cells to recognize molecular patterns of pathogenic microbial components. This research aims to identify the variability of the TLR4 gene and its relationship with blood profiles in local Indonesian chickens. The samples used to identify TLR4 gene diversity in local Indonesian chickens were 135 samples. Research methods include DNA extraction, PCR amplification, RFLP analysis, and genotype determination. The genotyping results in the research were associated with a complete blood profile so that a genotype was obtained that could be used as a marker for resistance traits based on the blood profile. The results of this study showed that the TLR4 gene was polymorphic with three genotype types and an allele frequency of more than 0.01 in each KUB, Sensi, and Broiler CP 707 chicken. The frequency of the GG genotype is the highest compared to the AG and AA genotypes. White blood cell concentration values showed variations in the three genotypes of TLR4 gene and were still within normal standards, although statistically the differences were not significant.
Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens Maskur, Maskur; Muhsinin, Muhammad; Rozi, Tapaul; Jan, Rahma; Kasip, L.
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6682

Abstract

Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) is a gene that regulates the body's resistance to illness. The genetic diversity of Kampung and KUB chicken population in Lombok island West Nusa Tenggara province was investigated based on g.3768T>C loci of NRAMP1 gene. Experimental animal consisted of 105 KUB and 67 Kampung chickens. The NRAMP1 gene polymorphisms in both population were identified using PCR-RFLP method. Genetic parameters of g.3768T>C loci were calculated using PopGene32. In this study, the locus g.3768T>C of the NRAMP1gene found three genotypes (TT, CT, CC) with the frequency of the C allele was higher than the T allele, respectively 0.709 and 0.210 in Kampung chicken, and 0.738 and 0.262 in KUB chicken. The mean value of observed heterozygosity (Ho) and expected heterozygosity (He) of g.3768T>C loci in both populations revealed low to medium genetic diversity, with the values 0.222 and 0.400, respectively. The mean value of observed heterozygosity is lower than expected, indicating that the population is heading towards a heterozygosity deficit and proving that the two populations are not in Hardy-Weinberg equilibrium. The mean PIC value of the NRAMP1 gene (g.3768T>C) in both chicken population was 0.319 and classified as fairly informative (0.50 > PIC > 0.25). Meanwhile, the value of the genetic differentiation index (Fst) for Kampung and KUB chicken population were 0.385 and 0.433, indicating that there is differentiation between two chicken population at the observed loci.
Body Weight and Morphometric Characteristics of KUB Chicken at 60 Days of Age Al Farizi, Muhammad Salman; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7319

Abstract

Kampung Unggul Balitnak Chicken (KUB) is a highly favored Indonesian native chicken breed among breeders because of its rapid development rate and ability to thrive in many situations. This study aimed to investigate KUB chickens' growth performance and morphometric parameters at 60 days of age, a crucial phase for determining their suitability as commercial broilers. Using an intensive rearing system, 100 KUB chickens were reared for 60 days in group cages. Body weight measurements were recorded weekly, and morphometric traits were measured after the study period. The study's findings indicated that the mean body weight of KUB chickens at 60 days of age was 710.53±16.76 grams, suggesting a favorable growth rate. In addition, the morphometric analysis revealed favorable physical characteristics, including an average chest length of 100.55±3.70 mm, chest width of 47.47±3.12 mm, wing length of 165.93±4.34 mm, back length of 164.9±4.26 mm, back height of 205.51±3.99 mm, shank length of 63.23±3.69 mm, and shank circumference of 35.76±1.48 mm. The results indicate that KUB chickens exhibit desirable body weight and morphometric traits at 60 days old, proving their suitability in commercial broiler farming. To summarize, this study offers significant information regarding the growth performance and physical characteristics of KUB chickens during a critical phase of their development. The findings highlight the breed's capacity to serve as a practical choice for commercial broiler production, enhancing the variety and long-term viability of the poultry sector in Indonesia.
Genetic Diversity of Bali Cattle Base on Two Microsatellite Loci - INRA032 and BM2113 Maskur, Maskur; Rozi, Tapaul; Rahmajan, Rahmajan; Kasip, Lalu; Muhsinin, M.
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8076

Abstract

Microsatellites are short tandem repeat (STR) sequences that consist of simple repeats and exhibit a high number of alleles at each genomic locus. The aim of this study was to examine genetic variation in Bali cattle and the population dynamics using microsatellite markers. Two microsatellite loci, INRA032 and BM2113 were amplified using PCR Total DNA samples from the genome of 60 Bali cattle, then The PCR products were analyzed through agarose gel electrophoresis, followed by staining with Ethidium Bromide (EtBr). The number and size of alleles that appeared on the gel, while the diversity and population dynamics were analyzed using Popgene version 1.31 and Gene Calc. In this study, The effective number of alleles for the two microsatellite loci in the Bali cattle population analyzed was 6.53, with an average of 3.27. The average PIC of the two microsatellite loci was 0.70, while the observed heterozygosity (Ho) exceeded the expected heterozygosity (He), with values of 0.975 and 0.695, respectively. This suggests an excess of heterozygosity in the population and indicates that the populations are not in Hardy-Weinberg equilibrium. The inbreeding coefficient was high, with negative Fis values of -0.38 for INRA032 and -0.43 for BM2113.
Association of Inducible Nitric Oxide Synthase (iNOS) Gene Diversity with Immunity Characteristics in Kampung Chicken Muhsinin, Muhammad; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8296

Abstract

The immune system of Kampung chickens is vital for maintaining health and productivity, especially in combating bacterial infections. Among the genetic components involved, the inducible nitric oxide synthase (iNOS) gene is crucial for producing nitric oxide (NO), a molecule with strong antimicrobial properties. This study investigated the association between iNOS (AluI│g.15056T>C) gene polymorphism and immune traits in Kampung chickens to enhance disease resistance. Blood samples from 100 Kampung chickens were genotyped using PCR-RFLP, and immune parameters such as leukocyte count, macrophage activity, and bacterial resistance were evaluated. Statistical analysis revealed significant differences among TT, TC, and CC genotypes. The CC genotype exhibited superior performance, including the highest macrophage activity (91.74±1.92%), activated macrophage capacity (2279.49), and bacterial death rate (60.81±3.54%). These findings suggest that the CC genotype enhances NO production, strengthening the immune response to bacterial pathogens like Staphylococcus aureus and Salmonella Pullorum. Additionally, the polymorphism contributes to improved genetic diversity and immune efficiency in Kampung chickens. The study highlights the potential of incorporating the iNOS CC genotype in selective breeding programs to produce chickens with enhanced resilience. Future research should focus on interacting environmental factors with iNOS expression to optimize its application in chicken production.
Identification of Diversity and Genetic Distance of Indonesian Local Chicken Strains Based on Myostatin Gene Muhsinin, Muhammad; Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8297

Abstract

Indonesian local chickens are essential genetic resources with significant potential for advancing the national poultry industry due to their high genetic diversity, reflecting adaptation to tropical environments and traditional farming systems. This study analyzed the genetic diversity of Kampung, Sentul, KUB, SenSi, and Broiler chickens based on the MSTN g.4842T>G locus. Blood samples from 150 chickens underwent PCR-RFLP analysis using the BsrI restriction enzyme targeting exon 2 of the MSTN gene. Parameters such as observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and Hardy-Weinberg equilibrium were assessed. Genetic distances were calculated using PHYLIP, and a dendrogram was constructed with UPGMA. The study identified two alleles (G and T) and three genotypes (GG, GT, TT), with the T allele being dominant, particularly in Broiler chickens (78.3%). Ho was lower than He in all populations, indicating a heterozygosity deficit. PIC values ranged from 0.282 (Broiler) to 0.367 (SenSi), classifying the MSTN locus as moderately polymorphic. Kampung and KUB chickens showed the closest genetic relationship, while Broiler chickens were the most distinct. These findings underscore the potential of local chickens in breeding programs for adaptability and productivity. Further research with additional markers is recommended to enhance sustainable poultry farming.
Identification of Single Nucleotide Polymorphism (SNP) in Exon 5 of the Prolactin Gene in Native Chickens Rozi, Tapaul; Maskur, Maskur; Jan, Rahma; Kasip, Lalu Muhammad; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8342

Abstract

The prolactin gene (PRL) in chickens is one of the genes that regulates broodiness and egg production in laying hens. Single Nucleotide Polymorphism (SNP) is a form of single nucleotide variation in the DNA base sequence at specific locations in the genome that can influence differences in quantitative traits in livestock. This study aimed to identify SNPs in the prolactin gene of native chickens. The research methods included DNA extraction, PCR amplification, and DNA sequence analysis for genotype determination of individuals. The results revealed four mutation points at positions g.8052 bp T>C, g.7886 T>A, g.7823 A>G, and g.8069 T>C. All mutation points in exon 5 were substitution mutations. Statistical analysis showed that all loci were polymorphic and the population was in Hardy–Weinberg equilibrium.
Sistem Informasi Usulan Pelaksanaan Anggaran Dan Pengelola Keuangan Di Bagian Perencanaan Ditjen PKTrans Maskur, Maskur
IJCIT (Indonesian Journal on Computer and Information Technology) Vol 5, No 2 (2020): November 2020
Publisher : LPPM Universitas Bina Sarana Informatika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.096 KB) | DOI: 10.31294/ijcit.v5i2.7788

Abstract

Pengelolaan usulan pelaksanaan anggaran dan pengelola keuangan yang berjalan saat ini pada Direktorat Jenderal Pengembangan Kawasan Transmigrasi (Ditjen PKTrans) masih dilakukan secara konvensional. Hal ini tentu membutuhkan biaya yang tidak sedikit serta memperlambat proses administrasinya. Berdasarkan permasalah tersebut, peneliti bermaksud membangun sistem informasi yang dapat memberikan kemudahan bagi Ditjen PKTrans dalam pengolahan data usulan, pencarian data dan pembuatan laporannya. Metode penelitian yang digunakan untuk mengumpulkan data dilakukan melalui studi kepustakaan, observasi dan wawancara dengan pihak-pihak terkait. Aplikasi dibangun menggunakan bahasa pemrogram JAVA, NetBeans, XAMPP. Penelitian ini menghasilkan sistem informasi berbasis online yang memberikan kemudahan dalam pengelolaan data dan pelaporan ke satuan kerja perangkat daerah tanpa batasan waktu dan jarak.The management of budget implementation proposals and financial managers currently running at the Directorate General of Transmigration Area Development (Ditjen PKTrans) is still carried out conventionally. This of course requires a lot of money and slows down the administrative process. Based on these problems, the researcher intends to build an information system that can make it easier for the Directorate General of PKTrans in processing the proposed data, searching for data and making reports. The research method used to collect data was carried out through literature study, observation and interviews with related parties. Applications built using the JAVA programming language, NetBeans, XAMPP. This study produces an online-based information system that provides convenience in managing data and reporting to regional work units without time and distance limitations.
TRAINING PENGGUNAAN “MENDELEY REFERENCE MANAGER” SEBAGAI ALAT MANAJEMEN REFERENSI Utami, Rachma Bhakti; Amalia, Yessy Yuliana; Permatasari, Faudina; Maskur, Maskur
Jurnal Pengabdian kepada Masyarakat Vol. 11 No. 1 (2024): JURNAL PENGABDIAN KEPADA MASYARAKAT 2024
Publisher : P3M Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/abdimas.v11i1.5320

Abstract

Kemajuan teknologi saat ini dapat dimanfaatkan oleh masyarakat luas, termasuk peneliti maupun mahasiswa. Salah satu kemajuan teknologi yang dapat dimanfaatkan khususnya dalam membantu membuat daftar pustaka adalah “Mendeley Reference Manager”. Mendeley merupakan salah satu dari beberapa alat manajemen referensi yang direkomendasikan baik jurnal nasional maupun jurnal bereputasi internasional. Pengabdian Kepada Masyarakat ini bertujuan untuk memberikan training penggunaan “Mendeley Reference Manager” untuk memudahkan pengguna dalam membuat daftar pustaka otomatis. Metode pengabdian dilaksanakan melalui ceramah online, instruksi tentang penggunaan Mendeley reference manager, praktik penulisan sitasi dalam artikel ilmiah, dan tanya jawab. Peserta dalam pengabdian kepada masyarakat ini berjumlah 18 orang dengan berbagai latar belakang. Hasil dari pengabdian kepada masyarakat ini adalah para peserta dapat melakukan install “Mendeley Reference Manager” , membuat collection, membuat group kolaborasi hingga menyusun daftar pustaka otomatis. Kedepannya diharapkan para pengguna khususnya penelit dan mahasiswa dapat membuat daftar pustaka otomatis, dengan lebih baik, efektif, efisien dan meminimalisir kesalahan.
PENDAMPINGAN MAHASISWA PROGRAM KAMPUS MENGAJAR ANGKATAN 7 DALAM UPAYA PENINGKATAN KEMAMPUAN LITERASI DAN NUMERASI DI SDN 3 REJOTANGAN KABUPATEN TULUNGAGUNG Maskur, Maskur; Prastika, Aprilia Indi; Noormasari, Audrey Berlian; Saputri, Lina Gustaria Fillinanti Diah; Ammalyah, Puput
Jurnal Pengabdian kepada Masyarakat Vol. 11 No. 1 (2024): JURNAL PENGABDIAN KEPADA MASYARAKAT 2024
Publisher : P3M Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/abdimas.v11i1.5443

Abstract

The Campus Teaching Batch 7 program aims to improve students' literacy and numeracy skills through student mentoring at SDN 3 Rejotangan, Tulungagung Regency. This activity examines the effectiveness of the mentoring program in overcoming the problem of low student literacy and numeracy skills. The methods used are observation, interviews, and pre- and post-assistance tests to measure changes in students' abilities. The results show that there is a significant improvement in students' literacy and numeracy skills after receiving intensive assistance from students. This program also had a positive impact on students' learning motivation, as seen from the increased participation and enthusiasm of students in learning activities. The mentoring students acted as facilitators, providing interesting and relevant materials, and using interactive and fun learning methods. In addition, the collaboration between student assistants, teachers, and parents also supports the success of this program. Student assistance in the Teaching Campus Program Batch 7 is effective in improving students' literacy and numeracy skills at SDN 3 Rejotangan. Recommendations are given to develop similar programs in other schools to improve the quality of basic education in Indonesia.
Co-Authors AA Sudharmawan, AA Abdul Hakim Abdul Mutalib Abubakar, Asnandar Adang Hardi Gunawan Adji Santoso Dradjat Adolong, Nixon Afandi, Yosi Ahmad Imam Abdullah Ahmad Mustamir Waris Al Farizi, Muhammad Salman Alexander Kurniawan Ali Sofyan Kholimi Ali, Moh Mudassir Alvian, Muhammad Andri Amalia, Yessy Yuliana Ammalyah, Puput Amrullah Amrullah Ane Fany Novitasari Anis Nismayanti Annisa Utami Anwar Anwar Anwar, M. Khorul Ariana, Tirta Ariska, Sindy Dwi Armayrishtya, Zhulvardyan Arpan, Arpan Arpin, Arpin Arshy Prodyanatasari Askha, Muhammad Baitul Asnawi Asnawi Asrori Asrori Az zuhri, Fahmi Muhammad Azahraty, Azahraty Bachtiar Bachtiar, Mochammad Yusuf Bakri, Mubarak Baroroh Lestari Basalamah, Salim Basuki , Beauty Kartikasari, Prima Boedi Rachman Budiman Budiman Bulkaini (Bulkaini) Cahya Nova Ardianto Cecep T. Rustendi Chaeruman Chaeruman Chalista, Igna Aquila Choliq, Arifana Nur Dede Kurniasih Dekawati, Ani Didik Dwi Prasetya Djajanto, Ludfi Djoko Kisworo Edi Yanto Edi Yanto, Edi Efendi, A. Irfan Ekaningsih, Nuri Jannati Wahyu Elvi Susanti Syam Endang Sarmini Enny Lestari Evi Dwi Wahyuni Fachry Abda El Rahman Fairusya, Nuha Farid , Muhammad Farida Akbarina Farman, Eka Fatimah Fatimah Fatkhuronji, Moch Fatkhuronji, Moh. Fauzi Ahmad Muda Ferdinand Salomo Leuwol Fiernaningsih, Nilawati Fithriyah Fithriyah Fitri S Kasim Fitria Martanti, Fitria Furqan Furqan, Furqan Gustiani, Gustiani Gustiyana, Gustiyana H., M. Rusydi Hadi Susilo, Sugeng Hadiono, Abdi Fauji Hadisurya, Sukma Putri Yoshanti Halimah, Euis Teti Haryono Haryono Hasran Hayatudin Hayatudin Hendrawan, Muhammad Afif Herijanto, Pudji Heru Utomo, Heru Heryanto Eryk, Irwan Hidayat, Deden Hidayatulloh, M. Arief Himma, Mahmudatul Hindasah, Ida Husnurrofiq, Husnurrofiq I Ketut Sumadi Ibni Hadjar Irfan Noor, Irfan Irfin, zakiyah Isdaryanti, Isdaryanti Iskandar, Hasyim Isti Hidayah Iwan Setiawan Jafar N, Muhammad Kamil Jarjani Usman K. Hashimoto Kamaruddin Kamaruddin Kamaruddin Kamaruddin Kamase, Jeni KARIMAH, ULA MASYRIFATUL Kasip, L. Kasip, Lalu Kasman Kasman Khalik, Muh Fihris Khayanti, Eka khuzaini, Khuzaini Kusumawardani, Mutiara Laekkeng, Mursalim Laksmi Andri Astuti Lalu Muhammad Kasip Lama, Yuyu Lamrens Leuwol, Ferdinand Salomo Lina Budiarti, Lina Listiyani, Iis Listiyani Lugendo, Dorine Lukito Prasetyo Mamlukhah, Mamlukhah Maskuri Maskuri Masnida Masnida Mayastika, Kania Moch. Fatkhuronji, Moch. Moh Dahlan Th Musa Moh. Harun Al Rosid Mohammad Noor Hidayat Mokodongan, Rusliy Muhammad Aminuddin, Muhammad Muhammad Arif Nur Huda Muhammad Syahrul Ulum Muhlis, M. Abdul Muhsinin, M. Muhsinin, Muhammad Mujib Mujib, Mujib Murni Murni Musmuliyadin, Musmuliyadin Nabila, Nabila Narasuci, Win Noormasari, Audrey Berlian Nur Aini, Lilies Nur Hayatin Nurfauziah, Suci Nurhadi Nurhalisa, Nurhalisa Nurkaenah , Nurkaenah Nurtjahjani, Fullchis Nurul Inayah Obianto, Agung Oktora, Yekie Senja Permatasari, Faudina Pertiwi, Ligwina Dita Petrus Demon Sili Pongoh, Arianti Prastika, Aprilia Indi Prayitno, Mustofa Aji Pribadi, Joni Purwoko Purwoko Putra, Amal Rezka Putra, Deny Qutara Rachma Bhakti Utami Rahma Jan Rahmajan, Rahmajan Rahman Rahman, Abd. Rahman Ramadhan, Bangga Prastya Rasyid, Muhammad Rusdi Ratlan Pardede Ridwan, Ridwan Riska Rahayu Riska Riska Risnandah, Yuan Rizki, Moh Rohmatulloh, Puri Rosdiana Rosdiana Ruslan Ruslan Rustan Efendi Safitri, Arnadya Amira Safrul Muluk Saldi Saldi, Saldi Sandra Kasim, Sandra Sandra Sandra Sanita Dhakirah Sanjaya, Muhammad Fahyu Saputri, Lina Gustaria Fillinanti Diah Septian Enggar Sukmana Siska Andriani Siti Aminah Soebana, Heri Solly Aryza Sri Setyowati Sri Wahyuni Suherman, Suherman Sukarne, Sukarne Sukmajaya, Yade Sukmajaya, Yade Sulaiman, Muntasir Sulastini, Sulastini Surahman Amin, Surahman Suropaty, Rifki Pujiansyah Surya, Adhi Sutaryono, Yusuf Syaad Patmanthara Syahrul Syahrul Syamsuddin Syamsuddin Syamsuhaidi, Syamsuhaidi Syamsul, Zulfiani Talo, Joni Loe Tamam, M. Badrut Tapaul Rozi Tjahjo Winanto, Tjahjo Toiba, Yesberlin Triningsih Triningsih Umi Khabibah Wahyu Widyananda Wahyudin Wahyudin Widjanarko Widjanarko, Widjanarko Widyanti, Rahmi . Widyastuti Widjaksana YANTO YANTO Yayan Tahyan Yono Sugiarto Yufis Azhar Yuliana Yuliana Yuningsih, Noneng Zamzami Zainuddin, Zamzami Zulkifli Zulkifli ‘Aini, Yulis Nurul