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Muhamad Syukur
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia
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Evaluation of Progress Selection of F2 - F6 Population, A Cross between Two Lowland Tomato Genotypes Sautomo, Mohammad Wahyu; Syukur, Muhamad
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.836 KB) | DOI: 10.29244/jtcs.4.1.32-40

Abstract

One of the parameters that can be measured from the activities of selection is progress selection. The population of a plant which has been selected is expected to be good against a derivative of a crop which was selected. The aim of this research is to study the results of progress selection varieties superior of tomato plants down the generations in the population F2 005001 until F6 005001-4-1-12-3 with a standard tomato cultivar, “Ratna”. The result showed that the selected genotypes have shown improvements over the standard cutivar in terms of weight per fruit, fruit weight per plant, the number of fruits per plant, fruit thickness and the age of harvested plants in the population F2-005001 until F6 005001-4-1-12-3. The mean against the character being observed in the population F2 005001 until F6 005001-4-1-12-3 indicated that the result is better if compared with both parents, P1 (SSH-5 ) and P2 (Intan) and the standard cultivar “Ratna”. The value of heritability a wider sense indicate its value being on each character of being selected, while the value of heritability in a more narrow sense showed a low value on every character. The value of progress against character selection of weights per fruit, the weight of the fruit per plant, the number of fruit per plant, thick flesh fruit and the age of harvest showed increased slow progress in a genotype F2 005001 followed by a period of rapid progress in a genotype F4 005001-4-1 and very slow in a genotype F6 005001-4-1-12-3.
INDUCED MUTATION BY GAMMA RAYS IRRADIATION TO INCREASE CHILLI RESISTANCE TO BEGOMOVIRUS Gaswanto, Redy; Syukur, Muhamad; Purwoko, Bambang Sapta; Hidayat, Sri Hendrastuti
AGRIVITA, Journal of Agricultural Science Vol 38, No 1 (2016): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i1.581

Abstract

Begomovirus infection has a significant impact of lowering chilli yield in Indonesia. A constraint of narrow genetic variability of chilli in Indonesia has made the mutation breeding program as a solution worth-pursuing in increasing the genetic variability. The objective of this study was to determine the LD50 point for each of the five irradiated chilli genotypes and the optimum dose of gamma irradiation in inducing chilli resistance to Begomovirus and other improved agronomical traits. The study was conducted in the Indonesian Vegetable Research Institute (IVEGRI) at Cikole-Lembang, elevation 1,200 m above sea level, from March to December 2013. Split plot design was used with genotype as main factor (Kencana, Lembang-1, SSP, Tanjung 2, Seloka) and irra-diation dosage as sub-factor (0, 200, 400, 600, 800 Gy). All treatments were replicated three times. The results showed that LD50 points of the five irradiated chilli genotypes were in the range of 422.64-629.68 Gy. There were some chilli genotypes in the population of M2 that had high coefficient variance genetic (CVG) and broad sense heritability (h2bs) value for disease incu-bation time. This could be used as resistance parameter to Begomovirus and improvement parameter of several agronomical traits.
INDUKSI KALUS DAN REGENERASI DUA VARIETAS TEBU (Saccharum officinarum L.) SECARA IN VITRO SUHESTI, SRI; KHUMAIDA, NURUL; WATTIMENA, G. A.; SYUKUR, MUHAMAD; HUSNI, ALI; HADIPOENTYANTI, ENDANG; HARTATI, RR. SRI
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n2.2015.77-88

Abstract

ABSTRAKPerbanyakan tebu umumnya dilakukan secara vegetatif mengguna- kan setek. Teknik ini mempunyai keterbatasan memproduksi jumlah bibit dalam skala besar. Dalam rangka mendukung peningkatan produktivitas, maka perlu pemenuhan bibit tebu dalam skala besar. Kultur jaringan merupakan   teknologi   alternatif   yang   dapat   dikembangkan   untuk pemenuhan bibit dalam jumlah yang banyak. Tujuan penelitian ini adalah mendapatkan formulasi media terbaik untuk induksi kalus dan regenerasi tebu varietas Kidang Kencana dan PSJT 941. Penelitian dilakukan di Laboratorium Unit Pengelola Benih Unggul Pertanian, Pusat Penelitian dan  Pengembangan  Perkebunan,  Bogor  dari  bulan  Februari  sampai September 2012. Penelitian terdiri dari tiga tahap, yaitu induksi kalus, regenerasi tunas dan perakaran, serta aklimatisasi. Bahan tanaman tebu yang digunakan adalah daun muda varietas Kidang Kencana dan PSJT 941 yang masih menggulung. Empat formulasi media digunakan pada tahap induksi kalus. Sementara itu, pada tahap regenerasi tunas dan perakaran menggunakan tujuh formulasi media. Aklimatisasi menggunakan media tanah steril dan kompos (2:1). Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial, terdiri atas dua faktor dan diulang sepuluh kali. Faktor pertama adalah varietas dan kedua adalah formulasi media. Hasil penelitian menunjukkan media induksi kalus terbaik untuk varietas  Kidang  Kencana  adalah 2,4-D 9 µM  +  Picloram  4,5  µM, sedangkan PSJT 941 adalah 2,4-D 13,5 µM. Media regenerasi dapat digunakan untuk menginduksi tunas sekaligus perakaran. Media regenerasi terbaik varietas Kidang Kencana dan PSJT 941 adalah IBA 2,46 µM + BAP 1,33 µM. Kedua varietas dapat diaklimatisasi di rumah kaca dengan tingkat keberhasilan tinggi (80-100%).Kata kunci: Saccharum officinarum, tebu, kultur jaringan, organogenesis,                 perbanyakan Callus Induction and Plant Regeneration of Two Sugarcane Varieties (Saccharum officinarum L.) through In VitroABSTRACTGenerally, sugarcane propagation was done by vegetative cuttings. The technique had limitation of producing seeds in a large scale. In order to increase productivity of sugarcane, it is required to provide sugarcane seeds in large scale. Tissue culture is an alternative technique that can be developed to provide the seeds. The objective of this research was toobtain the best formulations for callus induction and regeneration of Kidang Kencana and PSJT 941 varieties. The study was conducted in the Laboratory of Superior Farm Seeds Management Unit, Indonesian Agency for Agricultural Research and Development, Bogor from February until September 2012. The researches were carried out in three steps, name lycallus induction, regeneration of shoots and roots, and acclimatization. Explant  material  used  was  young  rolled  leaves  collected  from  two sugarcane  varieties (Kidang  Kencana  and  PSJT 941).  Four  media formulations used for callus induction, while seven media formulations used for shoots and roots regeneration. Acclimatization used sterile soil and compost (2:1). The experiment arranged in Factorial Completely Randomized Design with two factors and ten replications. The first factor was varieties and second factor was media formulations. The results showed that the best callus induction media for Kidang Kencana was 2.4- D 9 µM + Picloram 4.5 µM, while for PSJT 941 was 2.4-D 13.5 µM. Regeneration  media  could  induce both  shoots  and  roots.  The  best regeneration media for Kidang Kencana and PSJT 941 were IBA 2.46 µM + BAP 1.33 µM. They could be acclimatized successfully in green house with highly percentage (80-100%).Key words:  Saccharum officinarum,  sugarcane, tissue culture, organo- genesis, multiplication
Nonparametric Stability Analysis of Starch Content of Gamma Irradiated Cassava at Three Locations in West Java, Indonesia Agustina, Ferra Anggita; Khumaida, Nurul; Ardie, Sintho Wahyuning; Syukur, Muhamad
Journal of Tropical Crop Science Vol 7 No 02 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.771 KB) | DOI: 10.29244/jtcs.7.02.66-74

Abstract

Cassava is one of the largest starch producing tuber crops in Indonesia. Tapioca from cassava starch can be used as a raw materials for a variety of foods including biscuits, instant porridge, meatball, sausage, nuggets, and flour condiment. Breeding programs to improve cassava yield and starch content by using mutagen gamma rays irradiation was tested in four cassava generations (M1V4). Cassava is propagated by stem cuttings and the new characteristic obtained from gamma irradiation mutation is stable and can be passed from one generation to the next. Cassava mutants were obtained by evaluating the performance in different environmental conditions. The testing of yield adaptability and stability through a series of multi location is an important step before a new variety can be released. The aim of this research was to compare nonparametric stability and to evaluate the stability of the starch content of 16 genotypes (14 mutants and 2 varieties) in three locations in West Java Province i.e. Tapos (Depok), Cikarawang (Bogor), and Ciseeng (Bogor). Experiments were conducted in a completely randomized block design with three replications nested in each environment. Testing of yield stability in this study used four approaches with 10 parameters of nonparametric method. Starch content was estimated using gravimetric method. Based on frequency stability ranking of starch content from gravimetric method the G63142 genotype had the highest starch content estimation (29.99%), and the top five genotypes with high starch content were G63142, G61142, “Manggu”, G62151, and G63124. G61142 was categorized as genotype with static and dynamic stability, therefore this genotype is a very potential mutant to be released, whereas G63124 is categorized as a genotype with a static stability. Genotypes G63142, G62151, and “Manggu” had variable but relatively high starch content.
INDUKSI KALUS DAN REGENERASI DUA VARIETAS TEBU (Saccharum officinarum L.) SECARA IN VITRO SUHESTI, SRI; KHUMAIDA, NURUL; WATTIMENA, G. A.; SYUKUR, MUHAMAD; HUSNI, ALI; HADIPOENTYANTI, ENDANG; HARTATI, RR. SRI
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n2.2015.77-88

Abstract

ABSTRAKPerbanyakan tebu umumnya dilakukan secara vegetatif mengguna- kan setek. Teknik ini mempunyai keterbatasan memproduksi jumlah bibit dalam skala besar. Dalam rangka mendukung peningkatan produktivitas, maka perlu pemenuhan bibit tebu dalam skala besar. Kultur jaringan merupakan   teknologi   alternatif   yang   dapat   dikembangkan   untuk pemenuhan bibit dalam jumlah yang banyak. Tujuan penelitian ini adalah mendapatkan formulasi media terbaik untuk induksi kalus dan regenerasi tebu varietas Kidang Kencana dan PSJT 941. Penelitian dilakukan di Laboratorium Unit Pengelola Benih Unggul Pertanian, Pusat Penelitian dan  Pengembangan  Perkebunan,  Bogor  dari  bulan  Februari  sampai September 2012. Penelitian terdiri dari tiga tahap, yaitu induksi kalus, regenerasi tunas dan perakaran, serta aklimatisasi. Bahan tanaman tebu yang digunakan adalah daun muda varietas Kidang Kencana dan PSJT 941 yang masih menggulung. Empat formulasi media digunakan pada tahap induksi kalus. Sementara itu, pada tahap regenerasi tunas dan perakaran menggunakan tujuh formulasi media. Aklimatisasi menggunakan media tanah steril dan kompos (2:1). Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial, terdiri atas dua faktor dan diulang sepuluh kali. Faktor pertama adalah varietas dan kedua adalah formulasi media. Hasil penelitian menunjukkan media induksi kalus terbaik untuk varietas  Kidang  Kencana  adalah 2,4-D 9 µM  +  Picloram  4,5  µM, sedangkan PSJT 941 adalah 2,4-D 13,5 µM. Media regenerasi dapat digunakan untuk menginduksi tunas sekaligus perakaran. Media regenerasi terbaik varietas Kidang Kencana dan PSJT 941 adalah IBA 2,46 µM + BAP 1,33 µM. Kedua varietas dapat diaklimatisasi di rumah kaca dengan tingkat keberhasilan tinggi (80-100%).Kata kunci: Saccharum officinarum, tebu, kultur jaringan, organogenesis,                 perbanyakan Callus Induction and Plant Regeneration of Two Sugarcane Varieties (Saccharum officinarum L.) through In VitroABSTRACTGenerally, sugarcane propagation was done by vegetative cuttings. The technique had limitation of producing seeds in a large scale. In order to increase productivity of sugarcane, it is required to provide sugarcane seeds in large scale. Tissue culture is an alternative technique that can be developed to provide the seeds. The objective of this research was toobtain the best formulations for callus induction and regeneration of Kidang Kencana and PSJT 941 varieties. The study was conducted in the Laboratory of Superior Farm Seeds Management Unit, Indonesian Agency for Agricultural Research and Development, Bogor from February until September 2012. The researches were carried out in three steps, name lycallus induction, regeneration of shoots and roots, and acclimatization. Explant  material  used  was  young  rolled  leaves  collected  from  two sugarcane  varieties (Kidang  Kencana  and  PSJT 941).  Four  media formulations used for callus induction, while seven media formulations used for shoots and roots regeneration. Acclimatization used sterile soil and compost (2:1). The experiment arranged in Factorial Completely Randomized Design with two factors and ten replications. The first factor was varieties and second factor was media formulations. The results showed that the best callus induction media for Kidang Kencana was 2.4- D 9 µM + Picloram 4.5 µM, while for PSJT 941 was 2.4-D 13.5 µM. Regeneration  media  could  induce both  shoots  and  roots.  The  best regeneration media for Kidang Kencana and PSJT 941 were IBA 2.46 µM + BAP 1.33 µM. They could be acclimatized successfully in green house with highly percentage (80-100%).Key words:  Saccharum officinarum,  sugarcane, tissue culture, organo- genesis, multiplication
Pendugaan Parameter Genetik dan Seleksi Karakter Morfofisiologi Galur Jagung Introduksi di Lingkungan Tanah Masam Lubis, Khairunnisa; Sutjahjo, Surjono Hadi; Syukur, Muhamad; Trikoesoemaningtyas, Trikoesoemaningtyas
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.798 KB) | DOI: 10.21082/jpptp.v33n2.2014.p122-128

Abstract

Morpho-physiological characterization of sixteen inbred lines of maize originated from CIMMYT and from Indonesian Cereals Research Institute Maros, was carried out in Tenjo, Bogor, from January to April 2013. The objectives of this research were to (1) estimate the genetic parameters of morphophysiological characters, (2) obtain morpho-physiological characters that can be used as selection characters and (3) obtain tolerance inbred lines on acid soil condition. The experiment was conducted using a Nested Design with three replications, nested in selection environment. The first factor was environments, the second factor was genotypes. The two environments were acid soil with eAl 0.2 me/100 g (represented the optimum soil) and acid soil with eAl 1.87 me/100 g (represented the medium acid soil). The results showed that environment had significant effect on inbred lines performances. Characters of anthesis silk interval, number of seeds/cob, number of rows/cob, cob dry weight, seed dry weight and cob weight/plot could be used as selection characters for tolerance to medium acid soil condition; the inbred lines tested indicated high genetic variabilities and showed high heritability under such condition. Inbred line CLA 84, CLA 46, CLA 95 and 1042-13 each was found tolerant genotype on medium acid soil condition
Nucleotide Variations of Gamma Ray-Irradiated Chili Pepper Mutant Genotypes Based on Gene-Specific Primers Related to Fruit Size Character Nugroho, Kristianto; Trikoesoemaningtyas, Trikoesoemaningtyas; Syukur, Muhamad; Lestari, Puji
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p83-94

Abstract

Fruit size is a quantitative character controlled by several genes and mutations in those genes could lead to phenotypic changes in fruits. Single nucleotide polymorphism (SNP) is a molecular marker that could be used to detect mutation in genes at the nucleotide level that could not be done by conventional markers. The aim of this study was to analyze nucleotide variations, primarily SNP, in mutant chili pepper genotypes using gene-specific primers related to fruit size characters to measure the genetic changes occured, identify the SNPs that were associated with fruit size character, and analyze the phylogenetic among the mutant genotypes. Sanger sequencing was conducted on six mutant[K21]  chili pepper genotypes from M2 generation and Lingga as origin variety using five pairs of newly designed gene-specific primers corresponding to fruit size characters. A total of 41 base changes were identified in the gamma ray-irradiated chili pepper mutants, consisting of 22 insertions/deletions (indels) and 19 SNPs. As many as 17 nonsynonymous SNPs which caused amino acid changes, in contrast to the other 2 synonymous SNPs, were found in these mutant genotypes. Association analysis between identified SNPs to fruit length and diameter showed nonsignificant association, therefore the functional marker related to targeted gene could not be developed. The genotype L269 showed a separation from Lingga in dendrogram reflecting a large number of genetic changes, showed its potential as a new germplasm in development of improved variety in the future.
One method to obtain genetic information is the diallel cross analysis. The objective of this study was to eavluate the genetic parameters of six inbred pepper (Capsicum annuum L.) using full diallel crosses. The experiment was conducted at IPB Experiment Field, Cikabayan, Darmaga. The design was randomized complete block design (RCBD) using three replications as blocks. Data from generation F1 and parents were analyzed using the Hayman Method. Results indicated that no epistatic effects were si MUHAMAD SYUKUR; SRIANI SUJIPRIHATI; RAHMI YUNIANTI; . UNDANG
HAYATI Journal of Biosciences Vol. 17 No. 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.4.183

Abstract

One method to obtain genetic information is the diallel cross analysis. The objective of this study was to eavluate the genetic parameters of six inbred pepper (Capsicum annuum L.) using full diallel crosses. The experiment was conducted at IPB Experiment Field, Cikabayan, Darmaga. The design was randomized complete block design (RCBD) using three replications as blocks. Data from generation F1 and parents were analyzed using the Hayman Method. Results indicated that no epistatic effects were significant for all the traits assessed. Additive genetic effects were larger than the dominant effects for yield per plant, fruit length, and  diameter fruit traits. Dominant genetic effects were larger than the additive effects for fruit weight traits. Narrow-sense and broad-sense heritability were high for all the traits assessed. The character of the yield per plant, fruit weight and fruit diameter shows that there were more dominant genes in the parents. There were more recessive genes in parents for the fruit length character. IPB C7 parent was the most recessive genes containing control characters in the yield per plant. In the new improved varieties of high yielding, IPB C7 could be crossed with IPB C9. Employing individual or mass selection breeding should be successful in developing high-productivity lines in this population.
Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and G ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI; MUHAMAD SYUKUR; RAGAPADMI PURNAMANINGSIH; ROSSA YUNITA; ROHIM FIRDAUS
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.16

Abstract

Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and Gunung Putri (1540 m asl). Genotype 8 had the latest age of flowering in the three locations than the other genotypes, however, the growth and biomass were the lowest. Vegetative growth of Artemisia in Pacet and Gunung Putri was better than those in Cicurug. Genotype of 15 in Cicurug and 5A genotype in Gunung Putri and Pacet had higher wet and dry weight than that of two other associates. Based on plant biomass, 5 genotypes from Gunung Putri and Pacet i.e. 1D, 3, 5A, 14, and 15 genotypes were selected, as well as 5 genotypes i.e. 1D, 3, 4, 5A, and 15 genotypes from Cicurug. Analisys on artemisin content successfully obtained 5 selected somaclone lines i.e. 1B, 2, 4, 14, and 3 somaclones.
Correlation and Path Analysis Quantitative Character of 20 Genotype Chilli (Capsicum annuum L.) Yunandra Yunandra; Muhamad Syukur; Elza Zuhry; Deviona Deviona
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 2 No 1 (2020): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 2 No.1, 2020
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.608 KB) | DOI: 10.36378/juatika.v2i1.369

Abstract

ABSTRACT Chili high yield is the target of plant breeding. High yielding chili selection can be done through direct selection of yield characters and indirectly through other characters. Indirect selection is more efficient than the selection of one character. This research aims to study the relationship between quantitative characters with yield as well as obtaining the selection criteria. Correlation analysis results showed that the fruit weight, fruit diameter, pericarp thickness, leaf width, and leaf length were positively correlated and were very significant on yield. Based on the path analysis, characters that have a positive direct effect on yield are fruit weight, fruit diameter, leaf width, and leaf length, while pericarp thickness had a negative direct effect but had a large indirect effect on yield.
Co-Authors , Deviona, , , Misnen , Widodo , Yudiwanti , Yustiana ,, Rosminah ,, Sopiana ,, Usman . Kusmanto . Rustam . Sobir . UNDANG . Widodo Abdul Hakim Abdul Hakim Abdul Rahman Fajar Sidiq Abdulah Bin Arif Abdullah B. Arif Abdullah Bin Arif Abdullah bin Arif Abdullah Bin Arif Adriwan Siregar, Heri Ady Daryanto Agustina, Ferra Anggita Ahmad Fahrori Al Hasani Akbar Hidayatullah Zaini Akbar Hidayatullah Zaini Ali Djamhuri Amaliah, Wenny Anas Dinurrohman Susila Andi Takdir Makkulawu Andri Kusmayadi Anung Wahyudi Aprilianti, Devi Kurnia Arief Riza Wijaya, Arief Riza Arif Imam Suroso Arum Sholikhah Arya Yuda Pangestu Asep Setiawan Asril Siregar Awang Maharijaya Azahra, Salsabila Azis Rifianto Azmi, Tubagus Kiki Kawakibi Azmi, Yudia Bagas Akmala Putra Bambang S. Purwoko Bambang Sapta Purwoko Bintang, dan Maria Carolina Evelyn Chesaria, Nanda Constantin, Mondjeli Cucu Gunarsih Darmawan Asta Kusumah Dasumiati Desta Wirnas Dewa Ngurah Suprapta Dewi Sukma Diah Setiowati, Retno Dian Rakhmad Didik Ariyanto Didy Sopandie Dimas Purwo Anggoro Dinar Tri Soelistyowati Diny Dinarti Djayadiningrat, Muhamad Hyar Dwi Guntoro Eka Martha Della Rahayu Elza Zuhry Endah Retno Palupi Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Eny Widajati Erin Puspitarini Evriani Mareza, Evriani Fajar Sidiq, Abdul Rahman Faqih Udin Ghaling Achmad Giyanto Giyanto Giyanto, dan Gustiningsih, Dini HADIPOENTYANTI, ENDANG Hapshoh, Siti Hartati, RR. Sri Helfi Eka Saputra Herry Suhardiyanto Hesti Paramita Sari Hesti Paramita Sari Hidayat, dan Purnama Higuchi, Hirokazu HUSNI, ALI Ilham Dzikrillah Indra Gunawan Irma Isnafia Arief Irman Hermadi Irvansyah Irvansyah Iskandar Lubis Isna Tustiyani Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi JAJAH KOSWARA Jatsiyah, Venti Joko Ridho Witono Juharni Karlin Agustina Ketty Suketi Khaerin Nida Khairunnisa Lubis Kisman Kisman Kondo, Tomohiro KRISTIANTO NUGROHO, KRISTIANTO Kusmanto, . Laksono Trisnantoro Lestari, Puji Lestari, Sari Arie Lia Marliyanti Lia Marliyanti Linda Oktaviana Lisa Sentani Lubis, Khairunnisa Luky Adrianto Luluk Prihastuti Ekowahyuni M A Chozin MADE ASTAWAN Maharijaya, dan Awang MARIA BINTANG Marimin Marimin Marlina Mustafa, Marlina Maya Lestari, Maya Maya Melati Mercy Julinda Zebua Meuthia Rachmaniah Mohamad Rahmad Suhartanto Mu'arif, Mu'arif Muhamad Achmad Chozin Muhammad Hidayat Ginanjar, Muhammad Hidayat Muhammad Ridha Alfarabi Istiqlal Muhammad Ridha Alfarabi Istiqlal Muhammad Ridha Alfarabi Istiqlal Muhammad Rifqi Setio Aji Muhammad Syafi’i Muhammad Zairin Jr. Munarti Muthi'ah Khairun Nisa Muthi’ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Nadila, Dea Nadiyah Mawaddah Ayuningtyas Nanda Chesaria Nangi, Moh. Guntur Nasution, Amal Ma’rup Nazirwan Nazirwan Neng Neni Neni, Neng Ninuk Purnaningsih Nugroho, Kristianto Nugroho, Rizki Anjal Puji Nugroho, Syarul Nur Wulandari Nura ,, Nura Nurbani, Hilmi Nurul Khumaida Odang Carman Okti Syah Isyani Permatasari Peni Lestari Permatasari, Okti Syah Isyani Perwira, Prajna Jaya Prayudani, Ayu Putri Gitanjali PUJI LESTARI Punjung Medaraji Suwarno PURNAMA HIDAYAT Putra, Bagas Akmala Putri, Astrid Aditya Putri, Faradila Danasworo Qadir, Abdul R Neny Iriany RAGAPADMI PURNAMANINGSIH Rahman Rahman Rahmawati, Rika Sri Rahmi Henda Yani Rahmi Yunianti RAHMI YUNIANTI RAHMI YUNIANTI Raisa Baharuddin Ratna Ningsih rawati ,, rawati Redy Gaswanto, Redy Reswari, Helvi Ardana Ria Ismiatun Ria Putri Ridwani, Sobir Riti, Estriana Ritonga, Arya W Ritonga, Arya Widura Ritonga, Arya Widura Rohim Firdaus ROHIM FIRDAUS ROHIM FIRDAUS Rokhana Faizah Rokhana Faizah Rosa Yunita Rosa Yunita Rosminah, Rosminah ROSSA YUNITA RR. Ella Evrita Hestiandari Rtionga, Arya Widura Rudy Hermanto Sadewi Maharani, Sadewi Sahid, Zulfikar Damaralam Sandra Arifin Aziz Sari Dewi, Okky Tiara Sautomo, Mohammad Wahyu Sayekti, Tri Wahono Dyah Ayu Sentani, Lisa Shandra Amarilis Shelvie Nidya Neyman Siahaan, Gretty Febriola Sianturi, Clarissa Yolanda Sintho Wahyuning Ardie Siti Marwiyah Siti Marwiyah Sobir Sobir Sobir Sobir Sobir Sobir, dan SRI HENDRASTUTI HIDAYAT Sri Nurdiati Sri Sujiprihati Sri Wahyuni Sri Wening Sri Wening, Sri Wening Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI SRIANI SUJIPRIHATI Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Subandi Subandi Subekti, Isnani Suhaeb, Firdaus W. Suhartini, dan Tintin Suhesti, Sri Sukma, Dewi Sulassih, . Sundari, Fitria Suci Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suwarto Suwarto Suwarto Syafi’i, Muhammad Syaidatul Rosidah Syaidatul Rosidah Syarifah Iis Aisyah Tatiek Kartika Suharsi Tatiek Kartika Suharsi Tefa, Anna Tefa, Anna Tengku Laila Kamaliah Theresia Dame Angel Lie Manurung Trias Sitaresmi Trikoesoemaningtyas Undang Undang Undang, Undang Verry Riyanto Vicente, Tobias Moniz Vitria Puspitasari Rahadi Wahid Rifan Gustoro Wahyu Widiyono Waras Nurcholis WATTIMENA, G. A. Wenny Amaliah Widodo , Widodo . Widodo Widodo Widodo Widodo Widodo, dan Willy Bayuardi Suwarno Winda Saskia Sijabat Winning Son Ashari Wulan Rahmawati Yesy Mardianawati Yohanes Hendrianto Yudilastari, Tiara Yudiwanti Wahyu E. Kusumo Yuli Rahmadi, Hernawan Yunandra Yundari, Yundari Yursida Yusnita Sari, Yusnita Yusuf Sumaryana Zebua, Mercy Julinda