Claim Missing Document
Check
Articles

Biological Hazard on Multidrug Resistance (MDR) of Escherichia Coli Collected From Cloacal Swab of Broiler Chicken on Wet Markets Surabaya Nenny Harijani1 , Surya Jovinson Tansil Oetama2 , Koesnoto Soepranianondo3 , Mustofa Helmi Effendi1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12125

Abstract

Objective: The purpose of this study was to determine the multidrug resistance (MDR) profile of Escherichiacoli from broiler chicken from Wonokromo Market and Tambahrejo Market Surabaya to some antibioticgroups, namely streptomycin, levofloxacin, cefotaxime, trimethoprim and chloramphenicol.Materials and Methods: Seventy samples were taken from Wonokromo market and Tambahrejo market,each of which had thirty-five samples. Sampling was used cloaca swab technique. Then inoculated on EosinMethylene Blue Agar (EMBA) media, purification of bacteria on EMBA media, and tested biochemicallywith Sulfide Indol Motility (SIM) and Triple Sugar Iron Agar (TSIA) media.Antibiotic sensitivity test was used the Kirby-Bauer method. Muller-Hinton Agar (MHA) media incubation.Then the inhibition zone was measured according to the Clinical and Labortary Standard Institute (CLSI)standards. Detect positive multidrug resistance (MDR) bacteria characterized by resistance to ?3 types ofantibiotics.Results: Detection results of Escherichia coli multidrug resistance (MDR) at Wonokromo market was85.7%, and at Tambahrejo market was 51.4%. Presumptive Extended Spectrum Beta Lactamase (ESBL)producing E. coli at Wonokromo market was 14.3% and at Tambahrejo market was 2.9%.Conclusion: The high level of MDR of Escherichia coli from cloacal swabs of broiler chicken was a threatto public health and the environment, and is an important concern to reduce the rate of its spread
Profile of Multidrug Resistance (MDR) and Methicillin Resistant Staphylococcus aureus (MRSA) on Dairy Farms in East Java Province, Indonesia Sancaka Chasyer Ramandinianto1 , Aswin Rafif Khairullah2 , Mustofa Helmi Effendi3, 4, Eka Pramyrtha
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12157

Abstract

Staphylococcus aureus is one of the causes in the case of Foodborne Disease, with the resulting toxintriggering the occurrence of Foodborne Intoxication. The purpose of this study was to identify multidrugresistant patterns and detect Methicillin Resistance Staphylococcus aureus (MRSA) in cow’s milk in EastJava, Indonesia. A total of 170 cow’s milk samples were taken from dairy farms on 3 Districs, then thebacteria were cultured and purified using Mannitol Salt Agar media. Resistance tests were carried out with5 antibiotics on Mueller Hinton Agar media and Methicillin Resistant Staphylococcus aureus (MRSA) byOxacillin Resistance Screen Agar Base (ORSAB). The results showed there were 76 (44.7%) S. aureus, thenantibiotic testing showed 13 (17.1%) S. aureus isolates had multidrug resistant (MDR) properties on theantibiotics tested and 84.6% isolates with MDR confirmed MRSA properties by ORSAB. It was concludedthat the raw milk can be a potential reservoir for MDR and MRSA strains to threat public health.
PEMBUKTIAN HORIZONTAL TRANSFER OF RESISTANCE GENES MELALUI UJI SENSITIVITAS ANTIBIOTIKA PADA BAKTERI GENUS Staphylococcus DARI KASUS BOVINE MASTITIS Mustofa Helmi Effendi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/368

Abstract

The experiment was carried out to show the evidence of horizontal transfer of resistance genes by antibiotics sensitivity test on bacteria of genus of Staphylococcus from bovine mastitic cases. The first step of the experiment was to prepare pure culture of Staphylococcus aureus and Coagulase Negative Staphylococci. Milk samples were collected from mastitic cases at the afternoon milking time. Preparation of pure culture of isolates were confirmed by MS agar, hemolytic activity, catalase and coagulase test. Both isolates were tested by using antibiotics sensitivity test. The result showed that the both isolates of Staphylococcus aureus and Coagulase Negative Staphylococci from Surabaya dairy herd were resistant against erythromycin antibiotic. Based on the result, it can be concluded that there was horizontal transfer of resistance genes between both isolates from same udder of dairy cows.
Prevalensi dan Derajat Infeksi Cacing Saluran Pencernaan pada Itik Jawa (Anas javanica) di Dua Daerah Geografis Berbeda Dian Ayu Permatasari; Kusriningrum Rochiman; Tjuk Imam Restiadi; Sri Mumpuni Sosiawati; Endang Suprihati; Mustofa Helmi Effendi
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.647 KB) | DOI: 10.20473/jops.v4i1.20271

Abstract

The aim of this study was to determined the prevalence and infection disease degree of worm gastrointestinal on duck in two different geographical territories. The duck feces examination samples were using natif method, sedimentation, and floatation. The result of this examination of lowlands showed that 20% (12/60) ducks checked that infected by two worm species of nematoda, wich ia Ascaridia galli (11,7%), Heterakis sp. (8,3%), with the range of infection degree classified as low infection (0-500 EPG), while the examination results of highlands showed that 44% (33/75) ducks checked infected by two worm species of nematoda, which is Ascaridia galli (17,3%), Heterakis sp. (10,7%) and one species of trematoda, which is Echinostoma sp. (6,7%), with the range infection degree classified low infection (0-500 EPG). it can get both single or mix infection. The analysis result has shown that there was differences between lowlands and highlands on the prevalence and infection disease degree of worm gastrointestinal on duck, was highly significant (p<0,05).
Prevalence and Severity of Scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur City Veena Vasheene Vijaya Kumar; Mustofa Helmi Effendi; Nunuk Dyah Retno Lastuti; Nusdianto Triakoso; Maudika Pamela Fiorenza
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.653 KB) | DOI: 10.20473/jops.v6i1.34286

Abstract

This study intends to know the prevalence and crusting severity of scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur Rabbit Farm. Sarcoptes scabiei is a mite that has a predilection in the stratum corneum of the skin and caused scabies. Clinical symptoms were characterized by alopecia and crusting in the skin. This study presents a laboratory explorative research with a cross-sectional approach. Samples used rabbits in the farm that showed the clinical symptoms of scabies and the result of scraping skin in a native examination at the laboratory. Rabbits that positive scabies then performed scoring by the severity based on the region of the body and clinical symptoms. Results showed that from 140 rabbits in the population, the positive result is 13 rabbits were infected by scabies and there were 2 grades of crusting severity of scabies consisting of mild and moderate grade with separated 2 regions of the body. The low grade showed the alopecia and crusting in the face region while the medium grade in the face and foot region. The conclusion of this study is the number of prevalence scabies is 9.29% and crusting severity that low grade is 69.32% and medium grade is 30.78%.
Prevalensi dan Analisis Faktor Risiko Multidrug Resistance Bakteri Escherichia coli pada Ayam Komersial di Kabupaten Blitar: Prevalence and Risk Factors Analysis of Multidrug Resistance of Escherichia coli Bacteria in Commercial Chicken, Blitar District Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i1.74

Abstract

Multidrug resistance is a problem that is difficult to overcome in terms of treating infectious diseases. Multidrug resistance is the term used to describe when a bacteria is resistant to three or more different classes of antibiotics. Escherichia coli as a commensal bacterium which has multidrug resistance, this causes more issues because Escherichia coli can transfer its resistant properties to other bacteria within the poultry digestive tract. The observational study is used to determine the risk factors and to estimate the quantitative effects arising from various components that contribute to the emergence of a disease. The sampling in this study was carried out randomly through cloaca swabs from commercial chicken farms in Blitar and 345 samples were collected. Complementary data collection was carried out using questionnaires, interviews, and field observations. The results showed the incidence of multidrug resistance in commercial chickens in the Blitar District was 72.5%. There is a relationship between causative factors with the incidence of multidrug resistance in Escherichia coli bacteria that is significantly associated with positive risk factors. The strength of this relationship can be seen from the value of OR and RR, among others factors of chicken breed (OR = 3.07; RR = 1.34), breeder's education (OR = 2.3; RR = 1.29), type of livestock business (OR = 7.5; RR = 1.43), type of animal feed (OR = 1.91; RR = 1.2), veterinary support for livestock raising management (OR = 3.09; RR = 1.44). The reference variables are whether the antibiotics are administered by non-veterinarians (OR = 2.35) or by the TS (OR = 7.92), and whether there is an antibiotic administration program (OR = 3.16; RR = 1.47). The overseeing function of farm maintenance, management, and implementation of antimicrobial administration in commercial chicken farms needs to be improved, to increase breeders' awareness of the careful usage of antibiotics and controlling the incidence of antibiotic resistance.
Total plate count of commercial pasteurized milk sold by street vendors in Mulyorejo sub-district Surabaya Lailatul Maghfiroh; A. T. Soelih Estoepangestie; Tri Nurhajati; Nenny Harijani; Mustofa Helmi Effendi; Didik Handijatno
Journal of halal product and research (JPHR) Vol. 4 No. 2 (2021): Journal of Halal Product and Research (JHPR)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jhpr.vol.4-issue.2.65-70

Abstract

Food and nutritional quality of society is one of mine focuses of the government. Milk have complete nutrients but also can be the source food borne disease. The Veterinary Public Health itself has the responsibility to provide guarantees of food safety from animal resources which is called ASUH (safe, healthy, wholesome and halal) to be consumed by society. This study aimed to know the Total Plate Count of commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya. The study was conducted from January until February 2018 at the Laboratory of Veterinary Public Health Department, Faculty of Veterinary Medicine, Universitas Airlangga. Thirty samples of commercial pasteurized milk were bought from three street vendors then examined using Pour Plate Method of Total Plate Count. Total Plate Count result showed that the number of bacteria in commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya was 1.0 x 104 CFU/ml, that met the National Standard of Indonesia (SNI) 01-3951-1995.
Effect of Cinnamon (Cinnamomum burmannii) Bark Oil on Testicular Histopathology in Streptozotocin Induced Diabetic Wistar Male Rats Mustofa Helmi Effendi
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.294 KB)

Abstract

The purpose of this study was to determine the effect of cinnamon bark oil (CBO) on testicular histopathology. The variables taken were the diameter of the seminiferous tubules and the number of leydig cells in the testes of white male rats (Rattus norvegicus) which had been induced by streptozotocin. Twenty white male rats adult Wistar strains aged 2-3 months with an average weight of 150-250 grams were used in this study. The rats were divided into five experimental groups (4 rats each group) treated with a combination of streptozotocin and cinnamon bark oil (CBO) which were designed as follows: K(-), K(+), P1, P2, and P3. P is white rat injected with streptozotocin with a single dose of 45 mg / kg body weight intraperitonially, followed by therapy. K(-) without streptozotocin and only given drug solvent, K(+) given drug solvent, P1 treated with CBO 100 mg / kgBB orally, P2 treated with CBO 200 mg / kgBB orally, P3 treated orally with CBO 400 mg / kg orally for 14 days. White rat animals were then mutilated and testicular organs were taken for histopathological preparations using HE staining, then the diameter of the seminiferous tubules and the number of Leydig cells were calculated. Data were analyzed with One Way ANOVA and followed by Duncan's test. The results showed that streptozotocin had the potential to reduce the size of the seminiferous tubule diameter and the number of Leydig cells, while CBO at a dose of 400 mg / kgBW could increase the size of the seminiferous tubule diameter and the number of Leydig cells. It can be concluded that CBO can be used to reduce testicular dysfunction induced in diabetic rats.Key words: Cinnamon bark oil, Seminiferous tubule diameter, Leydig cells, Streptozotocin, Male rat.
Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.571 KB) | DOI: 10.21157/j.ked.hewan.v15i2.4817

Abstract

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.
Uji Resistensi Antibiotik Ampicillin pada Bakteri Escherichia coli yang di Isolasi dari Beberapa Peternakan di Surabaya Khoiru Indana; Mustofa Helmi Effendi; Soeharsono Soeharsono
Jurnal Peternakan Lingkungan Tropis Vol 3, No 1 (2020): Jurnal Peternakan Lingkungan Tropis
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jpltrop.v3i1.5416

Abstract

Resistensi bakteri terhadap antibiotik merupakan suatu yang alami. Bahaya resistensiantibiotik merupakan salah satu masalah yang terjadi juga pada dunia peternakansehingga mengakibatkan kerugian bagi para peternak. Terjadinyanya resistensiantibiotika ini disebabkan pemakaian antibiotika pada hewan sebagai pemacupertumbuhan yang mempunyai kontribusi terjadinya resistensi antibiotika. Ampisilinsalah satu jenis antibiotik golongan penisilin yang dilaporkan resisten terhadapEscherichia coli. Selain harganya murah, ampisilin sangat mudah didapat. Penelitian inibertujuan mengidentifikasi Escherichia coli yang diisolasi dari daging ayam padapeternakan di Surabaya yang resisten terhadap Ampisilin. Uji resistensi Ampicillinterhadap Escherichia coli menggunakan metode Kirby-Baurer. Berdasarkan hasil isolasidan identifikasi dari 40 sampel didapatkan 13 isolat yang dinyatakan resisten Escherichiacoli atau sebanyak 32,5 %.
Co-Authors A. T. Soelih Estoepangestie Adiana Mutamsari Witaningrum Adrenalin, Sruti Listra Agnes Theresia Soelih Estoepangestie Al Arif, M Anam Amri, Indah Amalia Anam Al Arif Anas Bad’rosana Prawindi Annisa, Suwaibatul Annise Proboningrat Arifah, Sitti Asri Rizky Asri Rizky Astuti, Elita Sriwidya Bambang Sektiari Lukiswanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Benjamin Christoffel Tehupuring, Benjamin Christoffel Benny Perdana Boedi Setiawan BUDI UTOMO Budiarto Budiarto Budiarto Bura, Maria Antonia Yersi Dua Chusniati, Sri Dadik Rahardjo Dadik Rahardjo, Dadik Dadik Raharjo Dameanti, Fidi Nur Aini Eka Puji Dewi, Aprilia Kurnia Dian Ayu Permatasari Didik Handijatno Elisa Herina Dimariwu Emy Koestanti Sabdoningrum Endah, Tri Endang Suprihati Eriza Rosalina Rochmah Fadlilah, Shinta Levea Ni'matul Fatmawati, Mira Fatmawati Fauziah, Ima Fedik Abdul Rantam Habibi, Muhammad Afif Hani Plumeriastuti Hasib, Abdullah Hernanda, Ary Setya Herry Agoes Hermadi Istiana, Izzatul Iwan Sahrial Hamid Kadek Rachmawati Kendek, Irfan Alias Khairullah, Aswin Rafif Khoiru Indana Kinasih, Kurnia Nisa Kurniasih, Dea Anita Ariani Kurniawan, Muhammad ‘Ahdi Kusala, Muhammad Khaliim Jati Kusriningrum Rochiman Lailatul Maghfiroh Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luthfiana, Nurul Masdiana C Padaga Maudika Pamela Fiorenza Mirni Lamid Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Ikmal Robih Mustika, Yolla Rona Muzhaffar, Adam Maulana Naseh, Naseh Nenny Harijani Nunuk Dyah Retno Lastuti Nusdianto Triakoso Pradika, Adhitya Yhoga Pratama, Bima Putra Purbowati, Tri Endah Putri, Mariana Febrilianti Resilinda Rahaju Ernawati Rahmahani, Jola Rahmandari, Dina Agylia Raissa, Ricadonna Ramandinianto, Sancaka Chasyer Rehman, Saifur Residiwati, Gretania Riza Zainuddin Ahmad Rochmah Kurnijasanti Safitri, Devi Ayu Saputra, Rakan Mahiid Sari, Fifin Kurnia Setiawan Koesdarto Sevitasari, Adelita Putri Sheila Marty Yanestria Sitti Arifah Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sri Pantja Madyawati Subagyo, Safira Fauziyyah Suherni Susilowati Sukmanadi, Mohammad Supriyadi Suryanie Sarudji Suwarno Suwarno Syah Putra, Akyun Rozaqi Syah, Ali Akram Tacharina, Martia Rani Tang, John Yew Huat Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati Tri Untari Tri Untari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Tyasningsih, Wiwiek Tyasningsih, Wiwik Veena Vasheene Vijaya Kumar Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibisono, Freshinta Jellia Widi Nugroho Wiwiek Tyasningsih Wiwik Misaco Yuniarti Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Zahro, Vida Seanita Zainul Abidin