Claim Missing Document
Check
Articles

Duodenum Histomorphology of Bacillus subtilis and Bacillus coagulan Isolate in Laying Hen Strain Isa Brown Muzhaffar, Adam Maulana; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Wibawati, Prima Ayu; Sukmanadi, Mohammad; Solfaine, Rondius
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48030

Abstract

Probiotics are live microbes that are used as supplementary feed that work to increase the good bacteria that live in the digestive tract by suppressing pathogenic microorganisms in the intestine so that good bacteria can grow can optimize the feed absorption process. This study aims to determine the effect of adding probiotics as feed additive Against the height and width of the duodenal villi of the laying chicken in early production. Probiotics can nourish the digestive tract and increase nutrient digestibility so that nutrient intake is met for livestock. Bacillus subtilis and Bacillus coagulans as a prophylaxis against bacterial diseases by balancing intestinal microbes, increasing digestibility, and secreting protease, lipase, and amylase enzymes. The purpose of this study was to determine the effect of probiotics Bacillus subtilis and Bacillus coagulans on duodenal histomorphology. Twenty-four layers were randomly divided into 4 groups. The control group was not given probiotics, groups P1, P2, and P3 were given probiotics of 2 ml, 4 ml, and 6 ml. Probiotics were given for 14 days. One-way ANOVA showed a significant difference in each group (P<0.05) and continued with the DUNCAN test. The average length and width of the duodenal villi in each treatment showed that the control group was significantly different from the P1, P2, and P3 groups. The P3 group had the highest average length of duodenal villi. The increase in villi height and villi width is due to the increase in short-chain fatty acids induced by probiotics. An increase in the height and width of villi indicates the absorption capacity of the small intestine, the work of digestive enzymes on the intestinal mucosa is more maximal in the intestine with a wider morphological size, so that absorption and growth are better. The conclusion from the research that has been done is that the use of Bacillus subtilis and Bacillus coagulans as probiotics has an effect on the layer duodenal villi which is characterized by an increase in the average length of the duodenal villi according to the increase in the probiotic dose.
Profile of Antibiotic Residue and Antibiotic Resistance in Broiler Chicken Meat in Indonesia: Public Health Importance Bura, Maria Antonia Yersi Dua; Effendi, Mustofa Helmi; Puspitasari, Yulianna
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.15360

Abstract

Keamanan pangan merupakan isu global dan menjadi perhatian bersama. Di bidang peternakan, penyalahgunaan antibiotik dalam praktik kesehatan hewan berdampak pada bahaya residu dan resistensi antimikroba pada produk pangan asal hewan bagi kesehatan masyarakat. Penelitian ini mengkaji profil residu dan resistensi antibiotik pada daging ayam broiler di Indonesia. Kajian dilakukan melalui tinjauan pustaka terhadap artikel penelitian yang dipublikasikan di jurnal ilmiah nasional dan internasional, prosiding seminar, dan laporan penelitian. Hasil penelitian mengungkapkan, dalam lima tahun terakhir (2018-2023) masih ditemukan residu antibiotik pada daging ayam broiler di beberapa daerah di Indonesia. Pada periode yang sama, resistensi antibiotik juga ditemukan pada sampel daging ayam broiler di beberapa wilayah Indonesia. Resistensi antibiotik yang ditemukan adalah pada satu jenis antibiotik dan beberapa jenis antibiotik (multidrug resistance) sekaligus. Temuan ini dapat dijadikan bahan evaluasi perbaikan manajemen rantai produksi dan distribusi produk daging ayam broiler dalam negeri tentang jaminan keamanan pangan asal hewan.
Effectiveness of the California Mastitis Test (CMT), Reductase Test, and Alcohol Test for Dairy Cows Subclinical Mastitis Detection Eriza Rosalina Rochmah; Dadik Raharjo; Sri Hidanah; Mustofa Helmi Effendi; Adiana Mutamsari Witaningrum; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51443

Abstract

Mastitis is an inflammation of the mammary glands caused by pathogenic bacteria Mastitis divided into 2 types, clinical mastitis which has symptoms of swelling and changes in the shape of the mammary gland, while subclinical mastitis does not indicate clear symptoms. Subclinical mastitis is a very high case in Indonesia, around 97-98%, while clinical mastitis is 2-3%. Effectiveness of the california mastitis test (CMT), reductase test and alcohol test to detect subclinical mastitis in dairy cattle. The number of samples taken was 60 samples of fresh dairy milk from 15 cattles in Medowo Village as much as 50 ml for each sample. The results showed that the test was very effective in identifying subclinical mastitis in dairy cattle. 15% positive results were obtained for subclinical mastitis using CMT, 10% of the milk quality was not good during the reductase test and 18.33% positive with the alcohol test from a total of 60 samples obtained.
COUNSELING ON REPRODUCTIVE DISORDERS MANAGEMENT IN FEMALE BEEF CATTLE LIVESTOCK GROUPS IN DALEGAN VILLAGE, PANCENG DISTRICT, GRESIK REGENCY Suprayogi, Tri Wahyu; Susilowati, Suherni; Hermadi, Herry Agoes; Al Arif, M Anam; Hernawati, Tatik; Mulyati, Sri; Suwanti, Lucia Tri; Maslachah, Lilik; Kurnijasanti, Rochmah; Lestari, Tita Damayanti; Effendi, Mustofa Helmi; Hernanda, Ary Setya; Supriyadi; Saputra, Rakan Mahiid; Dewi, Aprilia Kurnia
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.026-038

Abstract

Management of reproductive disorders in female beef cattle (Bos taurus) is a vital step in enhancing livestock productivity and animal welfare. This educational activity was conducted in Dalegan Village, Panceng District, Gresik Regency, aiming to assist farmers in improving cattle reproductive performance through feed technology and supporting the development of modern farming practices. The methods applied included counseling, practical field demonstrations, and interactive discussions with participants, presented in a qualitative descriptive format. The results indicated an improvement in farmers' knowledge of the causes, clinical signs, and management steps for reproductive disorders such as anestrus, dystocia, and endometritis, as evaluated through pre-test and post-test assessments. Additionally, the activity provided guidance on the importance of feed management, cattle reproductive health, and modern farm management. In conclusion, this counseling successfully increased farmers' awareness of cattle reproductive health, which is expected to support the productivity of beef cattle farming in the region.
Multi-Drug Resistant (MDR) Detection in Escherichia coli in Canary Birds (Serinus canaria) Imported from Malaysia Istiana, Izzatul; Effendi, Mustofa Helmi; Kurniawan, Muhammad ‘Ahdi; Sari, Fifin Kurnia; Purbowati, Tri Endah; Rahmandari, Dina Agylia; Mufasirin; Setiawan, Boedi; Rahardjo, Dadik
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.123-136

Abstract

The frequency of canary imports continues to increase every year. Antibiotic resistance is a global problem that threatens human and animal health worldwide. Human interaction with birds as pets is a public health concern because it has the potential to increase zoonotic diseases. This study was conducted to identify the antibiotic resistance of E. coli using fecal swab samples of imported canaries from Malaysia. Samples were grown on an EMBA medium for the isolation test, Gram staining test, and IMViC test were performed to continue the identification test -Kirby-Bauer diffusion test - to determine antibiotic sensitivity. Based on morphological culture, Gram staining, and biochemical tests, the sample examination results showed 18% (27/150) were positive for E. coli, 16% (24/150) showed the highest resistance to tetracycline, 15,33% (23/150) amoxicillin, 12,66% (19/150) trimethoprim-sulfamethoxazole, 6% (9/150) ciprofloxacin, and 14,66% (22/150) isolates were confirmed MDR because they were resistant to three to four antibiotics. Further efforts are needed to understand and address the factors that lead to antibiotic resistance in the context of animal and public health. Prudent management of antibiotic use and monitoring of antibiotic resistance needs to be improved to maintain animal health and prevent the risk of transmission of resistant bacteria to humans.
Multi-Drug Resistant (MDR) Detection in Klebsiella Pneumoniae in Canary Birds (Serinus canaria) Imported from Malaysia Endah, Tri; Effendi, Mustofa Helmi; Kurniawan, Muhammad ‘Ahdi; Istiana, Izzatul; Sari, Fifin Kurnia; Rahmandari, Dina Agylia
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.173-188

Abstract

Antimicrobial resistance (AMR) is a top priority for the WHO and the EU Commission, considering it a top 10 threat to global public health. This study provides an overview of the potential spread of Klebsiella pneumoniae, which exhibits multi-drug resistance (MDR), as a reservoir for the spread of resistance genes in the community. Samples were incubated on an MCA medium for isolation, followed by a Gram stain test and an IMViC test for further identification. The Kirby-Bauer diffusion test was used to determine antibiotic sensitivity. Based on the morphological characterization of the cultures, Gram stain results, and biochemical tests, it was found that, of the 150 samples isolated, 12 (8%) were positive for K. pneumoniae; 91.66% (11/12) of the isolates showed the highest level of resistance to amoxicillin, 83.33% (10/12) to tetracycline, 66.66% (8/12) to ciprofloxacin, and 66.66% (8/12) to trimethoprim-sulfamethoxazole. As many as 83.33% (10/12) were identified as MDR as they showed resistance to three to four types of antibiotics. Judicious use of antibiotics, including proper selection of antibiotics and monitoring of their usage patterns, is key to maintaining treatment effectiveness. Joint efforts from various parties are needed to optimize the use of antibiotics and minimize the risk of bacterial resistance.
IMPROVEMENT DAIRY CATTLE BARN MANAGEMENT AFTER FOOT AND MOUTH DISEASE (FMD) OUTBREAK IN MEDOWO, KEDIRI Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto, Budiarto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 2 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i2.2025.260-270

Abstract

The Foot and Mouth Disease (FMD) outbreak that began in 2022 caused significant losses for dairy farmers in Medowo Village, Kandangan District, Kediri Regency—impacting both livestock health and farmers' economic stability. To address these impacts, a community service program was carried out to improve farmers' knowledge and skills in dairy cattle housing management and biosecurity implementation. The program included training sessions, educational outreach, and technical assistance for 31 farmers from KUD Kertajaya. Evaluation was conducted through pre-test and post-test instruments to measure the effectiveness of the intervention. Pre-test results showed that only 6.5% of participants were in the "good–very good" category in terms of understanding FMD and livestock management. After the intervention, post-test results showed a significant increase, with 87% of participants reaching the "good–very good" level. Additionally, in terms of practical application of cattle management practices, 100% of participants demonstrated excellent understanding after the program. This intervention proved that an educational approach combining outreach and hands-on practice can significantly enhance farmers' awareness and skills. The improvement is expected to contribute to better livestock productivity and more effective disease control. The activity also involved undergraduate students as part of the Merdeka Belajar Kampus Merdeka (MBKM) program, fostering collaboration between academia and the farming community to strengthen local capacity.
Milk quality test using lactose, specific gravity and fat test approaches at the Kertajaya Village Cooperative Unit, Kediri Astuti, Elita Sriwidya; Effendi, Mustofa Helmi; Rahmahani, Jola; Suwarno, Suwarno; Sabdoningrum, Emy Koestanti; Tacharina, Martia Rani
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74045

Abstract

The quality of fresh milk based on SNI 2011 regarding fresh milk requirements includes physical, chemical and the number of microorganisms. One of the physical properties of milk can be seen from the density test. The chemical properties of milk can be seen from the lactose and fat rate. The lactose test is by means of titration of the filtrate resulting from filtering milk which is free from any content other than lactose. Density test using a lactodensimeter. Fat test using the Gerber method. From the results of research on the quality of cow's milk which was carried out using the lactose test approach, density and fat obtain average results of 3.98% of lactose rate, 1.0259 g/ml of density and 2.7% of the at rate in VUC Kertajaya is good and close to the criteria according to SNI 2011. This can affect the selling price of milk from farmers in VUC Kertajaya.
The Implementation of Hazard Analysis and Critical Control Point (HACCP) in The Frozen Chicken Meat Distribution Chain in Sikka Regency Dua Bura, Maria Antonia Yersi; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Rahardjo, Dadik; Tyasningsih, Wiwiek; Rachmawati, Kadek
VITEK : Bidang Kedokteran Hewan Vol. 14 No. 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.289

Abstract

Food safety has become a global issue. Hazard analysis and critical control points (HACCP) is a food safety assurance system in the world that emphasizes prevention efforts. This research is a qualitative descriptive epidemiological study which aims to examine the application of HACCP in the distribution chain of frozen chicken meat in Sikka Regency on the growth parameters of total bacteria, Salmonella, and antibiotic residues through field observations at supplier companies and retailers, as well as laboratory tests on 30 frozen chicken meat samples, and 30 fresh chicken meat samples as a comparison. Laboratory tests found antibiotic residues in 60% of samples (16.7% in fresh chicken meat), and microbial contamination in 33% of samples without Salmonella contamination (40% in fresh chicken meat without Salmonella contamination). Monitoring of antibiotic residues has not been carried out properly by supplier companies because the number of samples is very small, sales in markets without refrigeration facilities for up to five hours together with fresh chicken or near fresh fish allows contamination of chicken meat. HACCP principles have not been properly applied in the frozen chicken meat distribution chain. Guidance and regular monitoring are needed so that the quality of frozen chicken meat is maintained.
Antimicrobial effect of Methanol and Ethanol Extracts of Kembang Bulan (Tithonia diversifolia) Leaves against Staphylococcus aureus Plumeriastuti, Hani; Habibi, Muhammad Afif; Tehupuring, Benjamin Christoffel; Effendi, Mustofa Helmi; Estoepangestie, Agnes Theresia Soelih; Sarudji, Suryanie; Witaningrum, Adiana Mutamsari
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.97-104

Abstract

The purpose of this study was to determine how Staphylococcus aureus responded to Kembang Bulan (Tithonia diversifolia) leaf extract's antibacterial properties. Eight S. aureus bacterial isolates from three Surabaya dairy farms were used in this study. Tithonia diversifolia leaf extract at concentrations of 10%, 5%, 2.5%, 1.25%, and 0% were employed in the study's samples. The antibacterial activity of Tithonia diversifolia leaf extract was assessed in this work using the disk diffusion method and an inhibition zone estimated in millimeters. Based on the findings of this study, it was determined that Tithonia diversifolia leaf extract at 5% and 10% concentrations had 100% antibacterial activity against S. aureus in eight samples. As with S. aureus, Tithonia diversifolia leaf extract at concentrations of 0%, 1.25%, and 2.5% did not exhibit any antibacterial action.
Co-Authors A. T. Soelih Estoepangestie Adiana Mutamsari Witaningrum Adrenalin, Sruti Listra Al Arif, M Anam Amri, Indah Amalia Anam Al Arif Anas Bad’rosana Prawindi Annisa, Suwaibatul Annise Proboningrat Arifah, Sitti Asri Rizky Asri Rizky Astuti, Elita Sriwidya Bambang Sektiari Lukiswanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan BUDI UTOMO Budiarto Budiarto Budiarto Bura, Maria Antonia Yersi Dua Chusniati, Sri Dadik Rahardjo, Dadik Dadik Raharjo Dameanti, Fidi Nur Aini Eka Puji Dewi, Aprilia Kurnia Dian Ayu Permatasari Dian Ayu Permatasari Didik Handijatno Didik Handijatno Didik Handijatno Elisa Herina Dimariwu Endah, Tri Endang Suprihati Eriza Rosalina Rochmah Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Fatmawati, Mira Fatmawati Fauziah, Ima Fedik Abdul Rantam Habibi, Muhammad Afif Hani Plumeriastuti Harijani, Nenny Hasib, Abdullah Hernanda, Ary Setya Herry Agoes Hermadi Istiana, Izzatul Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kendek, Irfan Alias Khairullah, Aswin Rafif Khoiru Indana Kinasih, Kurnia Nisa Kurniasih, Dea Anita Ariani Kurniawan, Muhammad ‘Ahdi Kusala, Muhammad Khaliim Jati Kusriningrum Rochiman Lailatul Maghfiroh Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luthfiana, Nurul Masdiana C Padaga Maudika Pamela Fiorenza Mirni Lamid Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Ikmal Robih Mustika, Yolla Rona Muzhaffar, Adam Maulana Naseh, Naseh Nenny Harijani Nunuk Dyah Retno Lastuti Nusdianto Triakoso Permatasari, Dian Ayu Pradika, Adhitya Yhoga Pratama, Bima Putra Purbowati, Tri Endah Putri, Mariana Febrilianti Resilinda Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Rahmandari, Dina Agylia Raissa, Ricadonna Ramandinianto, Sancaka Chasyer Rehman, Saifur Residiwati, Gretania Riza Zainuddin Ahmad Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Safitri, Devi Ayu Saputra, Rakan Mahiid Sari, Fifin Kurnia Sarudji, Suryanie Setiawan Koesdarto Sevitasari, Adelita Putri Sheila Marty Yanestria Sitti Arifah Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sri Pantja Madyawati Subagyo, Safira Fauziyyah Suherni Susilowati Sukmanadi, Mohammad Supriyadi Suwarno Suwarno Syah Putra, Akyun Rozaqi Syah, Ali Akram Tacharina, Martia Rani Tang, John Yew Huat Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati Tri Untari Tri Untari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Tyasningsih, Wiwiek Tyasningsih, Wiwik Veena Vasheene Vijaya Kumar Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibisono, Freshinta Jellia Widi Nugroho Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Zahro, Vida Seanita Zainul Abidin