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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Fitopatologi Indonesia Vegetalika Jurnal Sain Veteriner Jurnal Ilmu Pertanian Jurnal Penelitian Saintek Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Natur Indonesia Jurnal Pendidikan Matematika dan IPA BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Jurnal Sains dan Teknologi BUNGAMPUTI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Hutan Tanaman Jurnal Riset Ekonomi & Bisnis Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Biotropika Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Biotechnology Biota Biology, Medicine, & Natural Product Chemistry Planta Tropika Bioeksperimen: Jurnal Penelitian Biologi Majalah Obat Tradisional Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Scientiae Educatia: Jurnal Pendidikan Sains BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Ilmiah KOMPUTASI Jurnal Biodjati PROCEEDING ICBS Biosaintifika: Journal of Biology & Biology Education Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik Bioedukasi: Jurnal Pendidikan Biologi Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) jurnal Ilmu Pertanian (Agricultural Science) INSIST (International Series on Interdisciplinary Research) Jurnal Teknologi Pertanian Andalas Jurnal Riset Pendidikan Kimia (JRPK) Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal JKFT Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Akuntansi Food ScienTech Journal Sains dan Matematika International Journal of Environment, Engineering, and Education Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal of Environment, Engineering & Education Pasundan International of Community Services Journal (PICS-J) ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Berkala Penelitian Hayati Journal of Innovation in Community Empowerment (JICE) Comsep : Jurnal Pengabdian Kepada Masyarakat Jurnal Jarlit Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Journal of Biodiversity and Biotechnology Filogeni: Jurnal Mahasiswa Biologi Jurnal Kedokteran Hewan Berkala Ilmiah Biologi Journal of Fisheries & Marine BIO-SAINS | Jurnal Ilmiah Biologi Indonesian Journal of Jamu BIOTROPIC Scientiae Educatia: Jurnal Pendidikan Sains
BUDI SETIADI DARYONO
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
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Genetic Diversity of Mudskipper Species (Periophthalmus spp.) from the Southern Coast of Java, Indonesia, Based on the Mitochondrial 16S Gene Arisuryanti, Tuty; Nuraliyah, Tasya; Dwijayanti, Vindi; Aji, Katon Waskito; Priyono, Dwi Sendi; Daryono, Budi Setiadi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1352-1362

Abstract

The genus Periophthalmus (Bloch and Schneider, 1801) comprises a diverse group of mudskippers within the family Oxudercidae and subfamily Oxudercinae. It includes 20 verified species globally, with 11 of these species originating from Indonesia. Among them, P. kalolo and P. argentilineatus are prevalent across seven major islands. However, P. novemradiatus is less documented, with limited distribution records. The morphological similarities among Periophthalmus spp. complicate recognition, often leading to misidentification. To address this challenge, this study investigated the phylogenetic relationships and genetic diversity among these three species, utilizing the 16S rRNA gene as a marker. PCR amplification of the DNA from samples collected from seven regions along Java's southern coast employed primers 16Sar and 16Sbr. Phylogenetic analysis revealed three monophyletic clades corresponding to each species, with genetic divergences ranging from 3.66 to 5.40%. P. argentilineatus displayed a relatively high intraspecific divergence of 0.21-2.26%, suggesting the potential identification of cryptic species within this lineage, even with a conserved gene like 16S rRNA. We identified 47 variable sites within the 552-bp 16S rRNA sequence, including four singleton sites and 43 parsimony-informative sites. We discovered a unique marker at position 172 that could serve as a genetic identifier for distinguishing these species. These findings suggest that the 16S rRNA gene has potential as an alternative marker for species identification while also revealing genetic diversity, thereby complementing or even serving as an alternative to the commonly used COI gene in Periophthalmus spp.
Systematic Review: Recent Developments in Magnetic-Nanoparticle-Based DNA Extraction Tjoa, Stanley Evander Emeltan; Mudasir, Mudasir; Suharyadi, Edi; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

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Abstract

Deoxyribonucleic acid (DNA) is a research object for various molecular fields of study, and DNA extraction is a basic procedure in molecular biology laboratories. DNA extraction methods are evolving, from several steps using organic solvents to recent technology using magnetic nanoparticles as adsorbents for the purification step. The use of magnetic nanoparticles for DNA extraction is magnetizing because it is simple without heavy machinery, such as centrifugation and organic solvents. The application of this method is extensive and diverse. This systematic review summarizes current development trends for some aspects of this DNA extraction system from the magnetic nanoparticle synthesis method, extraction protocol, and different types of samples used. The main purpose of this review is to provide an overall perspective of the use of magnetic nanoparticles in DNA extraction for researchers to start their exploration of this safe, simple, and rapid technique. In general, the most well-known magnetic nanoparticle preparation uses the coprecipitation method. The preferred particle is a plain and silica-coated magnetic nanoparticle using tetraethyl orthosilicate. The most common buffer systems for binding, washing, and elution are Polyethylene Glycol (PEG) and NaCl, 70% ethanol, and Tris–EDTA, respectively. The extraction system with magnetite can be used for various types of samples, ranging from human specimens to animal, plant, and bacterial specimens. The extraction yields using the magnetic-nanoparticle-based method were between 0.03 µg and 116.63 µg with a DNA purity ratio between 1.09 and 1.99.
BENTUK DAN UKURAN STOMATA PADA TIGA JENIS KELAMIN PEPAYA GUNUNG (Vasconcellea pubescens A.DC.) MENGGUNAKAN SCANNING ELECTRON MICROSCOPE (SEM) Laily, Ainun Nikmati; Purnomo, Purnomo; Daryono, Budi Setiadi; Purwantoro, Aziz
BIO-SAINS : Jurnal Ilmiah Biologi Vol 5 No 1 (2025): Bio-Sains - Jurnal Ilmiah Biologi | Maret 2025
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v5i1.4829

Abstract

Pepaya gunung (Vasconcellea pubescens A.DC.) merupakan salah satu spesies anggota Suku Caricaceae yang memiliki nilai ekonomi dan agronomi, terutama karena kemampuannya tumbuh di dataran tinggi dan potensinya sebagai bahan baku obat. Tanaman ini diketahui memiliki tiga jenis kelamin, yaitu jantan dioecious, betina dioecious, dan monoecious. Stomata berperan dalam proses fisiologis seperti fotosintesis, transpirasi, dan pertukaran gas. Variasi dalam karakter stomata, seperti bentuk dan ukurannya menunjukkan diferensiasi fisiologis antar individu. Penelitian ini bertujuan untuk mengkaji karakteristik stomata pada tiga jenis kelamin pepaya gunung menggunakan Scanning Electron Microscope (SEM). Analisis dilakukan untuk mengidentifikasi perbedaan karakter stomata yang meliputi bentuk dan ukuran pada permukaan daun tiap-tiap jenis kelamin. Sampel daun diambil dari tanaman yang telah teridentifikasi jenis kelaminnya, kemudian dipreparasi dan diamati menggunakan SEM untuk memperoleh citra dengan resolusi tinggi. Hasil pengamatan menunjukkan adanya variasi pada karakteristik stomata antar jenis kelamin. Tanaman jantan dioecious cenderung memiliki stomata dengan ukuran paling besar dengan rerata 41,09 ± 18,50 x 29,41 ± 16,34 µm sedangkan tanaman betina dioecious menunjukkan ukuran stomata yang lebih kecil dengan rerata 28,08 ± 39,16 x 19,44 ± 26,57 µm. Adapun tanaman monoecious menampilkan ciri-ciri morfologi stomata yang bersifat intermediat dengan rerata 24,06 ± 8,22 x 20,30 ± 8,81 µm. Temuan tersebut memberikan pemahaman mikromorfologis terkait diferensiasi kelamin pada V. pubescens serta potensi penggunaan karakter stomata sebagai penanda awal identifikasi jenis kelamin tanaman
Variation Symptoms and Resistance Response of Different Types on Orchids (Orchidaceae) Against Odontoglossum ringspot virus (ORSV) Infection Mahfut, Mahfut; Daryono, Budi Setiadi
International Series on Interdisciplinary Science and Technology Vol. 4 No. 2 (2019)
Publisher : Universitas Lampung

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Abstract

Odontoglossum ringspot virus (ORSV) is one of the viruses that are reported to infect the most orchids in Indonesia. Viral infections cause economic losses. This research was conducted to study the introduction of symptoms of infection that appear as the initial stages of disease control. The source of the virus is orchid leaves Phalaenopsis amabilis positively infected by ORSV collected from Magelang. Virus isolation was carried out by mechanical inoculation to indicator plants (Chenopodium amaranticolor and Nicotiana tabaccum). Furthermore, the results of inoculation were multiplied using various types of orchids (Dendrobium, Cymbidium, Chattleya, Phalaenopsis, Spatthoglotis, Liparis, and Pecteilis). Virus detection on orchid tissue was done trough the DAS-ELISA and observation of virus structure using TEM. The results showed that Pectelis sussanae (L.) Raf. was the only orchid plant that was immune against ORSV approached. Dendrobium sp., Cymbidium sp., Chattleya sp., Phalaenopsis sp., and Spathoglotis sp. showed a response of susceptible of ORSV severe infection symptoms, while Liparis sp. were resistant. The variation of resistance from several orchid plant against infection ORSV showed severe symptoms with the incubation periode was seen earlier. The observation of virus structure using TEM showed rigid road shape particle, 300 x 18 nm in size, which is general characteristic of Tobamovirus. This indicate an infection of ORSV is a dangerous disease and require serious control
INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Yusuf, Adib Fakhruddin; Latifah, Vida Rahma; Nurcahyati, Vivi Indah; Nurhasanah, Anggun Diyan; Widyasari, Adristi Shafa; Nainggolan, Ananto Puradi; Hasibuan, Aldy Riau Wansyah; Hidayat, Madyan Akmal; Karmilah, Karmilah; Pratiwi, Arini Dian; Aurantika, Rindu; Hasanah, Muslifah; Aristya, Ganies Riza; Handayani, Niken Satuti Nur; Arisuryanti, Tuty; Lesmana, Indra; Daryono, Budi Setiadi
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica Arimarsetiowati, Rina; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Semiarti, Endang
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 38 No. 2 (2022)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.510

Abstract

Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7Ëš55'' ' S, 113Ëš41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.
PROXIMATE, TANNIN, AND AMYLOSE IN SIX SORGHUM VARIETIES ACROSS TWO PLANTING SEASONS Muazam, Arif; Kristamtini, Kristamtini; Widyayanti, Setyorini; Nugraha, Yudhistira; Kasiamdari, Rina Sri; Setiadi Daryono, Budi
BIOTROPIA Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.2.2461

Abstract

ARTICLE HIGLIGHTS- Sorghum nutrition varies across varieties and growing seasons- Dry season increases starch and amylose content in some varieties- Rainy season enhances protein levels in sorghum grains- Genotype-environment interaction affects sorghum nutritional quality- Sorghum adaptation supports food diversification and climate resilienceABSTRACTSorghum (Sorghum bicolor (L.) Moench) is a potential staple food in Indonesia, offering an alternative to rice and corn due to its adaptability to marginal lands and drought tolerance. However, research on the impact of environmental factors, particularly different growing seasons, to the nutritional composition of sorghum varieties are still lacking. This study aimed to analyze the proximate contents of tannin and amylose in six sorghum varieties cultivated in two different planting seasons (wet and dry seasons). A Completely Randomized Design with three replications was applied in this experiment. The tested varieties included three newly developed superior varieties (Bioguma, Samurai, and Kawali) and three local varieties (Black Bonteb, Red Glutinous Sorghum, and KD4). The results showed that proximate composition varied across varieties and seasons. Starch content was generally higher in the dry season, with the highest observed in Kawali (64.16%) and Bioguma (63.11%), while the lowest was in Black Wareng (11.99%). Similarly, protein content peaked in Black Wareng (7.68%) and Red Glutinous Sorghum (7.37%), but was the lowest in Samurai (4.48%). Tannin levels also fluctuated by season, where Bioguma, KD4, and Kawali exhibited higher tannin content in the dry season, whereas Samurai, Red Glutinous Sorghum, and Black Bonteb had lower tannin levels. Notably, amylose content was significantly higher in Red Glutinous Sorghum (4.72%) and Black Bonteb (4.62%) across both seasons, contributing to their fluffier texture when cooked. These findings highlight the influence of environmental conditions on sorghum nutritional quality, reinforcing its potential for food diversification, including processed sorghum flour as a substitute for wheat flour.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Genetic Diversity and Phylogeny of Alocasia Longiloba Miq. Indonesia Accessions based on trnL-F Intergenic Spacer Region Asih, Ni Putu Sri; Purnomo; Daryono, Budi Setiadi
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 2 (2023): July
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i2.51022

Abstract

Alocasia longiloba Miq. is a member of the Araceae family, which was complex and has diverse leaf shapes, color, and venation. Studies on the diversity of A. longiloba focused mostly on morphological characteristics, while the genetic diversity of this species has never been published. This research aims to determine the genetic diversity and phylogeny of the Indonesian accession of A. longiloba based on molecular approaches using trnL-F IGS region. A total of 20 accesses of A. longiloba from Indonesia were sequenced then together with 11 DNA sequences of A. longiloba registered at the NCBI, were aligned with the Mesquite software. Genetic diversity was examined using DnaSP ver. 6.12.03. The phylogenetic tree was reconstructed by MEGA 11 using the Maximum Parsimony method and the Tree-Bisection-Regrafting (TBR) algorithm. Among 389 sites recorded, 356 site were invariable, 5 site were variable and 28 site were gaps or missing data. This genetic variation in the form of insertion, deletion, transition, and transversion and the deletion event has received the most contributions. Reconstruction of the phylogenetic tree resulted in A. longiloba being polyphyletic. The use of cpDNA with the trnL-F IGS region could not show the relationship between the Indonesian accession of A. longiloba, so further research is needed in other regions of chloroplast and nuclear DNA.
Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles Erfianti, Tia; Daryono, Budi Setiadi; Budiman, Arief; Suyono, Eko Agus
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.46193

Abstract

Abstract Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing  the  level  of  liquid waste pollutants  can be  done  biologically  by  using  microalgal  organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO­2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9'-cis-neoxanthin, dino xanthin, and fucoxanthin. Highlight Research The growth rate was higher when Euglena was cultivated under continuous illumination. The biomass productivity of Euglena increased significantly under continuous illumination. The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination.
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anisa Parazulfa Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Aries Bagus Sasongko, Aries Bagus Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Aurantika, Rindu Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Budy Wiryawan Catur Ahda Darojatun Darmastuti Deris Trian Rahmandhias Desi Oktaviani DIAH RACHMAWATI Diah Rachmawati Diah Rachmawati DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Tarwaca Susila Putra Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Fadilah Husnun Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidayat, Madyan Akmal Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Indra Lesmana Irma Nofitahesti Ishak, Muhammad Alif Ismail, Ismail Isna Mustafiatul Ummah Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kristamtini Kristamtini, Kristamtini Kurniawan, Febri Yuda Kusnanda, Prima Sekti Kyswantoro, Yunita Firdha Latifah, Vida Rahma Lily Arsanti Lestari Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Muazam, Arif Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Mumpuni, Nurpuji Nainggolan, Ananto Puradi Narzassi, Angga Bintang Nia Fararid Askar Niken Satuti Nur Handayani Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurcahyati, Vivi Indah Nurdilfa, Nurdilfa Nurhasanah, Anggun Diyan Ovami, Debbi Chyntia Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Pratama, Bernardinus Pratiwi, Arini Dian Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putri, Tiara Amelia Putro, Karso Suryo Putu Ayu Damayanti RABBANI, ANNAS Rachmawati, Novia Noor Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Roberdi, Roberdi Rohman, Randi Abdur Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Setyorini Widyayanti, Setyorini Shafura, Nabila Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Siti Subandiyah Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudaryadi, Ignatius Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, ‪Teuku Nanda Saifullah Sulaiman, ‪Teuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaiman‬, ‪Teuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supadmi, Francisca Romana Sri Supriyadi Supriyadi Susamto Somowiyarjo Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tony Liwang Tri Joko Tri Rini Nuringtyas Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Utin Elsya Puspita Utin Elsya Puspita Utomo, Condro Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Widyasari, Adristi Shafa Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yekti Asih Purwestri Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yudhistira Nugraha Yuli Astuti Yuliara, I Made Yusuf, Adib Fakhruddin Zolekhah, Dewi