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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Fitopatologi Indonesia Vegetalika Jurnal Sain Veteriner Jurnal Ilmu Pertanian Jurnal Penelitian Saintek Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Jurnal Natur Indonesia Jurnal Pendidikan Matematika dan IPA Prosiding Seminar Biologi Bioedukasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Jurnal Sains dan Teknologi BUNGAMPUTI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Hutan Tanaman Jurnal Riset Ekonomi & Bisnis Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Biotropika Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Biotechnology Biota Biology, Medicine, & Natural Product Chemistry Planta Tropika Bioeksperimen: Jurnal Penelitian Biologi Majalah Obat Tradisional Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Scientiae Educatia: Jurnal Pendidikan Sains BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Ilmiah KOMPUTASI Jurnal Biodjati PROCEEDING ICBS Biosaintifika: Journal of Biology & Biology Education Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) jurnal Ilmu Pertanian (Agricultural Science) INSIST (International Series on Interdisciplinary Research) Jurnal Teknologi Pertanian Andalas Jurnal Riset Pendidikan Kimia (JRPK) Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal JKFT Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Akuntansi Food ScienTech Journal Sains dan Matematika International Journal of Environment, Engineering, and Education Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal of Environment, Engineering & Education Pasundan International of Community Services Journal (PICS-J) ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Proceeding Biology Education Conference Berkala Penelitian Hayati Journal of Innovation in Community Empowerment (JICE) Comsep : Jurnal Pengabdian Kepada Masyarakat Jurnal Jarlit Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Filogeni: Jurnal Mahasiswa Biologi Jurnal Kedokteran Hewan Berkala Ilmiah Biologi Journal of Fisheries & Marine BIO-SAINS | Jurnal Ilmiah Biologi Indonesian Journal of Jamu BIOTROPIC
BUDI SETIADI DARYONO
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
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Black-Pigmented Marine Pseudomonas aeruginosa Exhibiting Anti-Bacterial Activity against Multidrug-Resistant (MDR) Wound Infection Bacteria Prastiyanto, Muhammad Evy; Darmawati, Sri; Daryono, Budi Setiadi; Retnaningrum, Endah
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.880-890

Abstract

The urgency of multidrug-resistant (MDR) bacterial infections in wounds is a significant concern due to the high prevalence of MDR in healthcare settings. Black pigmented marine bacteria, strain PS1C, were isolated from marine sediment on Awur Beach Jepara, Central Java, Indonesia, and cultured in the laboratory. The aim of this research includes molecular identification of strain PS1C, extracting black pigment from strain PS1C, isolation of MDR bacteria from wounds, and evaluating the antibacterial activity of black pigment from strain PS1C against MDR bacteria isolates of wound infections. We examined the 16S rRNA gene sequences strain PS1C to identify the species. Then, the black pigment from PS1C was extracted using methanol: acetone (7:3) solvent. Antibacterial activity was evaluated against MDR bacteria from wounds with the microdilution method. A black-pigmented bacterium was isolated and identified as Pseudomonas aeruginosa PS1C. We found that the black pigment from P. aeruginosa PS1C can be developed as an antibacterial agent against the MDR bacterial isolate of wounds with MIC and MBC values of 6.25-12.5 mg/ml and 6.25-25 mg/ml, respectively. In conclusion, the study's findings highlight the potential of the extract of black pigment from P. aeruginosa PS1C as an antibacterial agent against wound-causing MDR bacteria and reinforce previous research into P. aeruginosa can be isolated from marine sources. Additional in vivo investigations and the identification of the antibacterial activity's mode of action are required.
Tolerance of Melon (Cucumis melo L.) Cultivar Melodi Gama 3 to Powdery Mildew Infection Rozikin, Rozikin; Daryono, Budi Setiadi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3815

Abstract

Powdery mildew is one of melon disease caused by ascomycetes fungus from genus Sphaerotheca and Erysiphe. Breeding is considered to be the best approach to control Powdery mildew infection especially in melon. MG3 is a melon cultivar derived from ‘MG1’ x ‘Ladika’ and its estimate carry out resistant gene to Powdery mildew infection inherited from MG1. This research is to find out percentage and phenotype characters of Powdery mildew infection in MG3 compared to four commercial melons (Action 434, Glamour, MAI and ladika) that are popular in the market. Five melons are planted in greenhouse B of KP4 UGM. After five weeks, the plants where inoculated with Powdery mildew KP4 UGM in leaves. Scoring in three days after inoculation and the percentage of infections measured every 3 days for eight times using grid line 21 cm x 21 cm with small cubics 1,5 cm x 1,5 cm. The analysis of infections level in leaves, plant and population was done using Fukino protocol. The results show that cultivar MG3 tolerant and stable to Powdery mildew infection compare to cultivar Action 434, MAI and ladika in leaves, plant and population level. The infection of Powdery mildew caused damage to the leaves tissue, decreasing the abilities of photosynthesis resulting in necrotic, chlorotic and dead of the infected leaves. In fruits, infection causes break in the net, immature and rotten flesh, decrease sweetness and scent in cultivar Action434, MAI and Ladika. Base on microscopic analysis, Powdery mildew isolat KP4 UGM is a member of from genus Podosphaera.
Effects of Biocatharantine and Colchicine on Ploidy and Morphological Traits of Orange Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai ‘Citra Laga’) Setiyobudi, Rizal Hermawan; Yusuf, Adib Fakhruddin; Daryono, Budi Setiadi
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.4197

Abstract

Massive production of watermelon is correlated with its nutritional value and attractive appearance. Intensification of plant breeding through polyploidization is of utmost importance in horticulture to improve productivity with desirable morphological traits. Colchicine is a common polyploid inducer with limitations, including effects on plant viability and high costs. Another anti-mitotic agent has been developed from Catharanthus roseus ethanolic extract, but the applicative provisions need further exploration. This research focuses on the efficacy and performance of biocatharantine and colchicine in improving ploidy levels with morphological evaluation on orange watermelon ‘Citra Laga’. Mature seeds were treated with 2% biocatharantine and 0.2% colchicine for 12 and 24 hours. Generally, major morphological traits are unchanged, with significant differences found only in the width of leaves and color characteristics. Flow cytometry is performed to assess the ploidy level of plant cells. Mixoploid plants containing 2C+4C+8C DNA are produced by colchicine, but the lowest germination rate is found to have a negative effect. Biocatharantine increases the peak intensities of tetraploid 4C DNA within mixoploid plants with minimum risk to plant viability. This research discovers the potential of biocatharantine as an anti-mitotic agent, with an evaluation of the octaploid success of orange watermelon ‘Citra Laga’ mediated by colchicine.
Evaluation of Three Cucurbitaceae Cultivars Resistant against Begomoviruses Based on Morphological Symptoms and Molecular Analysis Subiastuti, Aprilia Sufi; Huda, Muhammad Syafi’atol; Daryono, Budi Setiadi
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.87915

Abstract

Cucurbitaceae is a group of annual horticultural crops with high economic prospects and benefits. The Faculty of Biology, Universitas Gadjah Mada has developed several new cultivars within the Cucurbitaceae family, such as the melon cultivar 'Hikapel', watermelon 'Citra Jingga', and butternut squash 'Citra Laga'. However, development of these cultivars has been hindered by yellow disease, typically triggered by Begomovirus infection. This study aimed to determine the morphological symptoms of yellow disease, to detect Begomovirus infection using molecular approach, and assess resistance levels of the three Cucurbitaceae cultivars. The method involved observing morphological symptoms in plants and measuring plant height. The degree of symptom severity was then converted into a vulnerability index (VI). The symptomatic leaf samples were tested using Krusty and Homer primer to confirm the symptoms caused by Begomovirus. The PCR results were sequenced and analyzed using Maximum Likelihood method in MEGA 11. The results showed that symptoms included curling tip of leaves, yellow mosaic on the leaves, and stunted growth in some plants, which were suggested as Begomovirus infection. Based on the Vulnerability Index (VI), the watermelon cultivar 'Hikapel' was considered susceptible to Begomovirus infection (VI = 85%), while the yellow squash 'Citra Laga' was moderately susceptible (VI = 57.5%). However, the watermelon 'Citra Jingga' was considered moderately resistant to this infection (VI = 50%). The presence of DNA the Begomovirus at three species Cucurbitaceae were characterized by a 550 bp band. The 'Hikapel' melon and 'Citra Laga' butternut were infected with Tomato leaf curl New Delhi virus (ToLCNDV), while the ‘Citra Jingga’ watermelon were infected with Squash leaf curl Philippine virus (SLCuPV).
Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia Mahfut, Mahfut; Daryono, Budi Setiadi; Somowiyarjo, Susamto
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.108 KB) | DOI: 10.14692/jfi.13.1.1

Abstract

Native orchid is one of Indonesian natural resources which play important role as parental materials in breeding program. Virus infection is one of the limiting factors in the cultivation of orchid. The purpose of this study was to detect Odontoglossum ringspot virus (ORSV) from native orchid. Symptomatic orchids were collected from 5 botanical gardens, i.e. Bogor, Cibodas, Purwodadi, Balikpapan, and Enrekang Botanical Gardens. Detection and identification was conducted by serological method using ORSV specific antisera, followed by RT-PCR and DNA sequencing. The serological test showed that 5 samples gave positive reaction against ORSV antiserum, i.e. Phalaenopsis amboinensis (KRB2) and Phalaenopsis amabilis (KRB12) from Bogor Botanical Garden, Phalaenopsis amabilis (KRP18) and Dendrobium salacence (KRP20) from Purwodadi Botanical Garden, dan Phalaenopsis modesta J. J. Sm. (KRBp5) from Balikpapan Botanical Garden. RT-PCR of the 5 samples using specific primer of ORSV coat protein gene was successfully amplified fragment DNA with size ± 474 bp. Homology analysis of those 5 ORSV isolates showed the highest index similiarity of 99.8% with corresponding sequences from 14 other ORSV isolates. Phylogenetic analysis indicated that ORSV KRB2 and KRP18 isolates was clustered in a separate group far from ORSV isolates in other countries. This is the first report of ORSV infection on native orchids collection from 5 botanical gardens in Indonesia.
Downy Mildew Infection in Indonesian Melon Cultivar 'Melona' Based on Morphological and Anatomical Characters Angellya, Bunga Finovel; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8597

Abstract

Melons are a horticultural crop of the Cucurbitaceae family with high economic value and worldwide distribution. The 'Melona' variety is the result of breeding selection from commercial melons in Indonesia and has a golden yellow skin color with lobes, crisp flesh, and a high degree of sweetness. Downy mildew is a fungal infection that attacks the leaves of the plant causing brownish-yellow-colored symptoms. Downy mildew can cause crop failure under extreme conditions as the plant loses its productive capacity. Infection levels were observed by scoring and calculating disease index scores. The morphology of healthy and diseased plants was documented and analyzed descriptively. Anatomical features of healthy and diseased leaves were compared using anatomical observations on leaf cross-sections. Quantitative data analysis was carried out using the T-test analysis method with a 5% significance level. Melona's resistance to downy mildew is in the tolerant to susceptible category. Chlorosis and necrotic spots on the leaves and stunted growth are symptoms of downy mildew infection. Healthy leaves had a greater axial and abaxial epidermal thickness, a lower mesophyll thickness, a smaller cell size and a better quantity and quality of trichomes than leaves infected with downy mildew.
Keanekaragaman ektoparasit dan pengaruhnya terhadap produktivitas telur ayam F2 Mahkota Arab Narzassi, Angga Bintang; Nizma, Nata Dwi Annisa; Tsani, Salma Mutiara; Poerwanto, Seonarwan Hery; Daryono, Budi Setiadi
Jurnal Penelitian Saintek Vol 28, No 2 (2023)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i2.66808

Abstract

Penelitian ini bertujuan untuk mengetahui jenis ektoparasit yang ditemukan, mengetahui produktivitas telur, serta mempelajari pengaruh ektoparasit terhadap produktivitas telur ayam F2 Mahkota Arab. Tahapan penelitian yang dilakukan yaitu; pemeliharaan ayam, pengambilan data telur, pengambilan sampel ektoparasit, preparasi ektoparasit, dan identifikasi ektoparasit. Analisis data meliputi jumlah ektoparasit per-inang, dominasi jenis ektoparasit, dan jumlah kumulatif telur (9 minggu). Hubungan jumlah ektoparasit dengan telur diuji dengan analisis korelasi Pearson (IBM SPSS Statistic 25) dengan taraf signifikansi 5%. Hasil penelitian menunjukan adanya 3 jenis ektoparasit yaitu Dermanyssus galliane, Menopon gallinae, dan Lipeurus caponis. Rerata jumlah telur ayam F2 Mahkota Arab (43.5 butir) lebih tinggi dari ayam Pelung (20 butir) sebagai salah satu ayam lokal. Hasil analisis korelasi pearson menunjukkan korelasi negatif antara jumlah total ektoparasit pada setiap pengambilan terhadap jumlah telur ayam F2 Mahkota Arab dengan nilai signifikansi sebesar 0,008 (P<0,05).Kata kunci: ektoparasit, F2 Mahkota Arab, produktivitas telurThe diversity of ectoparasites and its effect  on the egg productivity of F2 Mahkota Arab Abstract: This research aimed to identify the types of ectoparasites found, determine the egg productivity, and study the influence of ectoparasites on the egg productivity of F2 Mahkota Arab chickens. The research stages include chicken maintenance, egg data collection, ectoparasite sampling, ectoparasite preparation, and ectoparasite identification. Data analysis covers the number of ectoparasites per host, dominant ectoparasite species, and cumulative egg count over 9 weeks. The relationship between ectoparasites and egg productivity is tested using Pearson correlation analysis (IBM SPSS Statistics 25) with a significance level of 5%. The research results show the presence of three ectoparasite species: Dermanyssus galliane,Menopon gallinae, and Lipeurus caponis. The average number of F2 Mahkota Arab chicken eggs (43,5 eggs) is higher than that of Pelung chickens (20 eggs), one of the local chicken breeds. The Pearson correlation analysis indicate a negative correlation between the total number of ectoparasites in each collection and the number of F2 Mahkota Arab chicken eggs, with a significance value of 0.008 (P<0.05). Keywords: Ectoparasite, F2 Mahkota Arab, egg productivity
Cucumis melo L. ‘Gama Melon Parfum’ Extract: Volatile Compound Profile, Optimization, Preparation and Perfume Radar Gunawan, Indra; Daryono, Budi Setiadi; Noviana, Eka; Sulaiman, Teuku Nanda Saifullah
Majalah Obat Tradisional Vol 30, No 1 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95553

Abstract

Perfume notes are a sophisticated blend of essential or fragrance oils that give perfumes a distinct scent. Melon notes are highly favored aromatic elements in perfumes. Melon (Cucumis melo L. ‘Gama Melon Parfum’), known by the local name Gama Melon Parfum (GMP), is a melon breed that has unique phenotypic characteristics: bitter taste, batik-like pattern, and fragrant aroma. GMP has a fresh and fruity melon character, typically used as a top note in the perfume pyramid concept. Our study aimed to develop a perfume derived from GMP volatile extract as a fragrance ingredient. The process included collection, identification, extraction, physicochemical characterization, volatile compound analysis, perfume optimization, preparation, and perfumery radar. GMP can serve as the top note in a perfume formulation. From the D-optimal mixture design, an ideal perfume formula could be created from a mixture of 0.95 mL GMP, 0.55 mL rose, and 0.50 mL vanilla. The composition was found to produce a perfume that met sensory standards: a transparent and uniform liquid without impurities. It also had a unique aromatic scent with a relative density of 0.8102 g/cm3 which fell within the specified range of 0.7000-1.200 g/cm3, viscosity of 0.7931 cps within the required range of 0.7830-1.4030 cps, and a hedonic level of 9.72 cm out of a maximum of 15.00 cm. The perfume radar showed fruity-floral-musk characteristics. Results from this research indicate that enhancing GMP as a fragrance ingredient enables the creation of an optimal perfume that meets the required standards, is well-received, and demonstrates an inclination toward femininity.
Genetic Diversity of Mudskipper Species (Periophthalmus spp.) from the Southern Coast of Java, Indonesia, Based on the Mitochondrial 16S Gene Arisuryanti, Tuty; Nuraliyah, Tasya; Dwijayanti, Vindi; Aji, Katon Waskito; Priyono, Dwi Sendi; Daryono, Budi Setiadi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1352-1362

Abstract

The genus Periophthalmus (Bloch and Schneider, 1801) comprises a diverse group of mudskippers within the family Oxudercidae and subfamily Oxudercinae. It includes 20 verified species globally, with 11 of these species originating from Indonesia. Among them, P. kalolo and P. argentilineatus are prevalent across seven major islands. However, P. novemradiatus is less documented, with limited distribution records. The morphological similarities among Periophthalmus spp. complicate recognition, often leading to misidentification. To address this challenge, this study investigated the phylogenetic relationships and genetic diversity among these three species, utilizing the 16S rRNA gene as a marker. PCR amplification of the DNA from samples collected from seven regions along Java's southern coast employed primers 16Sar and 16Sbr. Phylogenetic analysis revealed three monophyletic clades corresponding to each species, with genetic divergences ranging from 3.66 to 5.40%. P. argentilineatus displayed a relatively high intraspecific divergence of 0.21-2.26%, suggesting the potential identification of cryptic species within this lineage, even with a conserved gene like 16S rRNA. We identified 47 variable sites within the 552-bp 16S rRNA sequence, including four singleton sites and 43 parsimony-informative sites. We discovered a unique marker at position 172 that could serve as a genetic identifier for distinguishing these species. These findings suggest that the 16S rRNA gene has potential as an alternative marker for species identification while also revealing genetic diversity, thereby complementing or even serving as an alternative to the commonly used COI gene in Periophthalmus spp.
Systematic Review: Recent Developments in Magnetic-Nanoparticle-Based DNA Extraction Tjoa, Stanley Evander Emeltan; Mudasir, Mudasir; Suharyadi, Edi; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Deoxyribonucleic acid (DNA) is a research object for various molecular fields of study, and DNA extraction is a basic procedure in molecular biology laboratories. DNA extraction methods are evolving, from several steps using organic solvents to recent technology using magnetic nanoparticles as adsorbents for the purification step. The use of magnetic nanoparticles for DNA extraction is magnetizing because it is simple without heavy machinery, such as centrifugation and organic solvents. The application of this method is extensive and diverse. This systematic review summarizes current development trends for some aspects of this DNA extraction system from the magnetic nanoparticle synthesis method, extraction protocol, and different types of samples used. The main purpose of this review is to provide an overall perspective of the use of magnetic nanoparticles in DNA extraction for researchers to start their exploration of this safe, simple, and rapid technique. In general, the most well-known magnetic nanoparticle preparation uses the coprecipitation method. The preferred particle is a plain and silica-coated magnetic nanoparticle using tetraethyl orthosilicate. The most common buffer systems for binding, washing, and elution are Polyethylene Glycol (PEG) and NaCl, 70% ethanol, and Tris–EDTA, respectively. The extraction system with magnetite can be used for various types of samples, ranging from human specimens to animal, plant, and bacterial specimens. The extraction yields using the magnetic-nanoparticle-based method were between 0.03 µg and 116.63 µg with a DNA purity ratio between 1.09 and 1.99.
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anisa Parazulfa Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Aries Bagus Sasongko, Aries Bagus Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Aurantika, Rindu Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Budy Wiryawan Catur Ahda Darojatun Darmastuti Deris Trian Rahmandhias Desi Oktaviani Diah Rachmawati Diah Rachmawati DIAH RACHMAWATI DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Tarwaca Susila Putra Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Fadilah Husnun Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidayat, Madyan Akmal Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Indra Lesmana Irma Nofitahesti Ishak, Muhammad Alif Ismail, Ismail Isna Mustafiatul Ummah Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Karmilah, Karmilah Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kristamtini Kristamtini, Kristamtini Kurniawan, Febri Yuda Kusnanda, Prima Sekti Kyswantoro, Yunita Firdha Latifah, Vida Rahma Lily Arsanti Lestari Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Muazam, Arif Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Mumpuni, Nurpuji Nainggolan, Ananto Puradi Narzassi, Angga Bintang Nia Fararid Askar Niken Satuti Nur Handayani Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurcahyati, Vivi Indah Nurdilfa, Nurdilfa Nurhasanah, Anggun Diyan Ovami, Debbi Chyntia Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Pratama, Bernardinus Pratiwi, Arini Dian Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putri Renata, Nellis Nadinda Putro, Karso Suryo Putu Ayu Damayanti Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Roberdi, Roberdi Rohman, Randi Abdur Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Setyorini Widyayanti, Setyorini Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Siti Subandiyah Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, ‪Teuku Nanda Saifullah Sulaiman, ‪Teuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaiman‬, ‪Teuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supadmi, Francisca Romana Sri Supriyadi Supriyadi Supriyadi Supriyadi Susamto Somowiyarjo Susilawati Susilawati Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tony Liwang Tri Rini Nuringtyas Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Utin Elsya Puspita Utin Elsya Puspita Utomo, Condro Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Widyasari, Adristi Shafa Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yekti Asih Purwestri Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yudhistira Nugraha Yuli Astuti Yuliara, I Made Yusuf, Adib Fakhruddin Zolekhah, Dewi