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Variation Symptoms and Resistance Response of Different Types on Orchids (Orchidaceae) Against Odontoglossum ringspot virus (ORSV) Infection Mahfut, Mahfut; Daryono, Budi Setiadi
International Series on Interdisciplinary Science and Technology Vol. 4 No. 2 (2019)
Publisher : Universitas Lampung

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Abstract

Odontoglossum ringspot virus (ORSV) is one of the viruses that are reported to infect the most orchids in Indonesia. Viral infections cause economic losses. This research was conducted to study the introduction of symptoms of infection that appear as the initial stages of disease control. The source of the virus is orchid leaves Phalaenopsis amabilis positively infected by ORSV collected from Magelang. Virus isolation was carried out by mechanical inoculation to indicator plants (Chenopodium amaranticolor and Nicotiana tabaccum). Furthermore, the results of inoculation were multiplied using various types of orchids (Dendrobium, Cymbidium, Chattleya, Phalaenopsis, Spatthoglotis, Liparis, and Pecteilis). Virus detection on orchid tissue was done trough the DAS-ELISA and observation of virus structure using TEM. The results showed that Pectelis sussanae (L.) Raf. was the only orchid plant that was immune against ORSV approached. Dendrobium sp., Cymbidium sp., Chattleya sp., Phalaenopsis sp., and Spathoglotis sp. showed a response of susceptible of ORSV severe infection symptoms, while Liparis sp. were resistant. The variation of resistance from several orchid plant against infection ORSV showed severe symptoms with the incubation periode was seen earlier. The observation of virus structure using TEM showed rigid road shape particle, 300 x 18 nm in size, which is general characteristic of Tobamovirus. This indicate an infection of ORSV is a dangerous disease and require serious control
Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica Arimarsetiowati, Rina; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Semiarti, Endang
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 38 No. 2 (2022)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.510

Abstract

Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7Ëš55'' ' S, 113Ëš41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Genetic Diversity and Phylogeny of Alocasia Longiloba Miq. Indonesia Accessions based on trnL-F Intergenic Spacer Region Asih, Ni Putu Sri; Purnomo; Daryono, Budi Setiadi
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 2 (2023): July
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i2.51022

Abstract

Alocasia longiloba Miq. is a member of the Araceae family, which was complex and has diverse leaf shapes, color, and venation. Studies on the diversity of A. longiloba focused mostly on morphological characteristics, while the genetic diversity of this species has never been published. This research aims to determine the genetic diversity and phylogeny of the Indonesian accession of A. longiloba based on molecular approaches using trnL-F IGS region. A total of 20 accesses of A. longiloba from Indonesia were sequenced then together with 11 DNA sequences of A. longiloba registered at the NCBI, were aligned with the Mesquite software. Genetic diversity was examined using DnaSP ver. 6.12.03. The phylogenetic tree was reconstructed by MEGA 11 using the Maximum Parsimony method and the Tree-Bisection-Regrafting (TBR) algorithm. Among 389 sites recorded, 356 site were invariable, 5 site were variable and 28 site were gaps or missing data. This genetic variation in the form of insertion, deletion, transition, and transversion and the deletion event has received the most contributions. Reconstruction of the phylogenetic tree resulted in A. longiloba being polyphyletic. The use of cpDNA with the trnL-F IGS region could not show the relationship between the Indonesian accession of A. longiloba, so further research is needed in other regions of chloroplast and nuclear DNA.
Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles Erfianti, Tia; Daryono, Budi Setiadi; Budiman, Arief; Suyono, Eko Agus
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.46193

Abstract

Abstract Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing  the  level  of  liquid waste pollutants  can be  done  biologically  by  using  microalgal  organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO­2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9'-cis-neoxanthin, dino xanthin, and fucoxanthin. Highlight Research The growth rate was higher when Euglena was cultivated under continuous illumination. The biomass productivity of Euglena increased significantly under continuous illumination. The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination.
Pemanfaatan pekarangan dan limbah rumah tangga untuk budidaya lele sebagai upaya memenuhi pangan keluarga selama pandemi covid-19 Subiastuti, Aprilia Sufi; Daryono, Budi Setiadi; Sukirno, Sukirno
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5 No 1 (2022)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i1.11323

Abstract

Pandemi Covid-19 menyebabkan perubahan kondisi sosial ekonomi masyarakat Indonesia. Sekitar 40% pekerja atau pelaku usaha mengalami perubahan pendapatan. Hal ini sedikit banyak mempengaruhi kemampuan masyarakat dalam memenuhi kebutuhan pangan keluarga. Pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada masyarakat untuk mengoptimalkan pemanfaatan pekarangan dan limbah rumah tangga untuk memenuhi kebutuhan pangan keluarga melalui budidaya lele. Pelaksanaan program dibagi ke dalam tiga tahap, yaitu pelatihan warga, pendampingan, dan evaluasi.Pelatihan warga meliputi pelatihan budidaya lele dan kangkung secara hidroponik menggunakan pot dan pelatihan pembuatan pakan lele dari limbah rumah tangga. Pendampingan dilakukan secara berkala setiap minggu hingga lele bisa dipanen. Evaluasi dilakukan di akhir kegiatan untuk mengetahui efektivitas dan potensi teknologi untuk dikomersilkan. Hasil dari program ini menunjukkan bahwa budidaya lele dan kangkung menggunakan pot dapat digunakan untuk memenuhi kebutuhan pangan keluarga dengan harga yang murah. Desain teknologi kolam portabel dari pot ini juga berpotensi untuk dikembangkan sebagai industri rumah tangga. Pakan lele dari limbah rumah tangga mampu menekan biaya pakan dalam budidaya lele hingga 20% dengan nilai rasio konversi pakan (FCR) sebesar 1.11 atau efisiens pakan hingga  90%. Pakan lele ini membantu pengurangan sampah sisa makanan dan sampah dapur hingga 100%.
Pembuatan sediaan lotion dari ekstrak melon Kultivar Gama Melon Parfum (GMP) di Laboratorium Farmasi Universitas Gadjah Mada Sunusi, Fitriani Auriga; Makmur, Kurnia; Sulaiman‬, ‪Teuku Nanda Saifullah; Sartika, Dian; Daryono, Budi Setiadi; Hajrah, Hajrah
Filogeni: Jurnal Mahasiswa Biologi Vol 3 No 2 (2023): Mei-Agustus
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v3i2.34885

Abstract

Indonesia memiliki beragam tumbuhan yang kaya akan manfaat salah satunya sebagai bahan dalam industri kosmetik. Bahan alami dipilih karena memiliki keuntungan yang ramah lingkungan dan rendah iritasi. Salah satu bahan alami yang saat ini sedang dikembangkan untuk dijadikan sebagai bahan kosmetik adalah dengan menggunakan ekstrak melon kultivar Gama Melon Parfum (GMP) dari Universitas Gadjah Mada. Tujuan penelitian ini untuk mengetahui proses pembuatan sediaan lotion dari ekstrak melon kultivar Gama Melon Parfum di Laboratorium Farmasi, Universitas Gadjah Mada. Metode yang digunakan adalah penelitian eksperimental, yaitu penelitian yang terfokus pada percobaan pembuatan sediaan lotion dengan ekstrak melon diperoleh melalui metode decoct (pemanasan) dengan beeswax dan cetyl alcohol dimasukkan ke dalam paraffin liquid dan diaduk hingga membentuk lotion. Hasil yang diperoleh yaitu tidak didapatkan konsistensi yang sesuai pada lotion atau mengalami kegagalan disebabkan oleh basis yang tidak cocok dan beberapa faktor lainnya.
Pembuatan sediaan gel dari ekstrak melon (Cucumis melo L. “Gama Melon Parfum”) di Laboratorium Farmasi Universitas Gadjah Mada Nurdilfa, Nurdilfa; Meriem, Selis; Sulaiman, ‪Teuku Nanda Saifullah; Yusuf, Adib Fakhruddin; Daryono, Budi Setiadi
Filogeni: Jurnal Mahasiswa Biologi Vol 4 No 1 (2024): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v4i1.34887

Abstract

Kosmetika bahan alam saat ini menjadi penggunaan yang sangat esensial misalnya penggunaan Gama Melon Parfum sebagai bahan baku pembuatan gel, karena penggunaan tumbuhan sebagai bahan dasar kosmetika memiliki bahan baku yang aman untuk digunakan dan memiliki efek samping yang lebih kecil. Gama Melon Parfum merupakan melon yang saat ini dikembangkan oleh Universitas Gadjah Mada yang dapat dijadikan sebagai bahan baku kosmetik alami karena memiliki metabolit sekunder berupa flavonoid, saponin, terpenoid dan cucurbitacin. Penelitian ini bertujuan untuk mengetahui proses pembuatan gel dari ekstrak Gama Melon Parfum. Metode yang digunakan yaitu metode eksperimental, yang terdiri atas pembuatan ekstrak melon melalui metode dekokta (pemanasan) dan pembuatan gel. Adapun hasil yang didapatkan yaitu sediaan gel dengan banyak gelembung udara di dalamnya disebabkan karena proses pengadukan yang terlalu cepat dan banyaknya udara yang masuk pada saat pembuatan sediaan gel. Hal ini menunjukkan proses pembuatan gel harus diperhatikan agar mendapatkan sediaan gel yang sesuai.
Pembuatan sediaan serum dari ekstrak Gama Melon Parfum (GMP) di Laboratorium Farmasi Universitas Gadjah Mada Masita, Masita; Masriany, Masriany; Sulaiman, ‪Teuku Nanda Saifulllah; Wibowo, Wiko Arif; Daryono, Budi Setiadi
Filogeni: Jurnal Mahasiswa Biologi Vol 4 No 1 (2024): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v4i1.34997

Abstract

Kosmetik menurut Food and Drug Administration merupakan produk yang digunakan pada kulit dengan tujuan untuk membersihkan, mempercantik, memperbaiki diri ataupun untuk meningkatkan daya tarik. Berdasarkan Peraturan Kepala BPOM nomor 18 tahun 2015 tentang persyaratan teknis bahan kosmetik, bahan kimia pada kosmetik dibedakan menjadi dua, yaitu bahan kimia berbahaya yang dilarang dan bahan kimia berbahaya yang diperbolehkan digunakan dengan pembatasan dalam penggunaannya pada produk kosmetik. Namun pada dasarnya, kosmetik tidak hanya terbuat dari bahan kimia melainkan dapat pula terbuat dari bahan alami seperti ekstrak buah yang dimanfaatkan. Salah satu tumbuhan yang dapat dijadikan bahan alami dalam pembuatan kosmetik yaitu buah melon. Jenis penelitian merupakan penelitian kualitatif yang terfokus pada proses pembuatan sediaan serum dari ekstrak melon kultivar Gama Melon Parfum yang diperoleh melalui metode dekokta (pemanasan). Hasil yang diperoleh yaitu diketahui adanya batasan-batasan dalam pembuatan kosmetik terkhusus pada penambahan bahan dan perlakuan yang membantu keberhasilan pembuatan serum, seperti pengadukan campuran harus dilakukan secara konstan, jika tidak maka akan menimbulkan buih atau gelembung yang menyebabkan pH serum tidak stabil.
PECTORALIS THORACICUS MUSCLE PERFORMANCE OF HYBRID CHICKEN (F1) DERIVED FROM CROSSBREED BETWEEN BROILER AND PELUNG (Gallus gallus gallus) Saragih, Hendry Tri Sakti; Roosdianto, Iwan; Daryono, Budi Setiadi
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.2282

Abstract

This research purpose was to study the phenotype characters of body weight and pectoralis thoracicus muscle performance of hybrid chicken (F1) derived from crossbreed between parent stock broiler strain Cobb-500 and Pelung (Gallus gallus gallus). The chicks were divided into 3 groups and each group consists of 5 days-old chicks (DOC). Group 1 (T1) was used as control involving DOC derived from crossbreed between female and male of broiler Cobb-500 strain. Group 2 (T2) involving DOC derived from crossbreed between female and male Pelung, while Group 3 (T3) was DOC derived from crossbreed between female broiler strain Cobb-500 and male Pelung. The chicken was grown up to 49 days to evaluate the body weight, the pectoralis thoracicus weight, the pectoralis thoracicus area, and myofiber area. Data were statistically analyzed using one way Anova. The results showed that the average of body weight in T1; T2; and T3 were 2,17086.02; 506.220.02; and 1,23868.25 g, respectively (P0.05). The average of pectoralis thoracicus muscle weight in T1; T2; and T3 were 165.0411.97; 21.262.44; and 68.741.94 g, respectively (P0.05). The average of pectoralis thoracicus muscle area in T1; T2; and T3 were 97.09,7; 616,4; and 63,41,8 cm2 , respectively (P0,05). The average of pectoralis thoracicus myofiber area in T1; T2; and T3 were 14.721.04; 4.662.1; and 13.131.3 m2 , respectively (P0.05). It was concluded that crossbreed between broiler strain Cobb-500 and Pelung improved the body weight and pectoralis thoracicus muscle performance of hybrid chicken (F1).
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Aries Bagus Sasongko, Aries Bagus Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Budy Wiryawan Catur Ahda Darojatun Damayanti, Putu Ayu Darmastuti Deris Trian Rahmandhias Desi Oktaviani DIAH RACHMAWATI Diah Rachmawati Diah Rachmawati DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Tarwaca Susila Putra Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol Husnun, Fadilah I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Irma Nofitahesti Ishak, Muhammad Alif Ismail, Ismail Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kurniawan, Febri Yuda Kusnanda, Prima Sekti Kyswantoro, Yunita Firdha Latifah, Vida Rahma Lily Arsanti Lestari Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahardhika, I Wayan Swarautama Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Mumpuni, Nurpuji Narzassi, Angga Bintang Nia Fararid Askar Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurdilfa, Nurdilfa Ovami, Debbi Chyntia Pabendon , Marcia Bunga Parazulfa, Anisa Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putro, Karso Suryo RABBANI, ANNAS Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ramlah Ramlah Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Roberdi, Roberdi Rohman, Randi Abdur Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Siti Subandiyah Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, ‪Teuku Nanda Saifullah Sulaiman, ‪Teuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaiman‬, ‪Teuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supadmi, Francisca Romana Sri Supriyadi Supriyadi Susamto Somowiyarjo Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tony Liwang Tri Rini Nuringtyas Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Ummah, Isna Mustafiatul Utin Elsya Puspita Utin Elsya Puspita Utomo, Condro Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yekti Asih Purwestri Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yuli Astuti Yuliara, I Made Yusuf, Adib Fakhruddin Zolekhah, Dewi