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Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.) Maryanto, Sigit Dwi; Rohman, Randi Abdur; Roberdi, Roberdi; Nuringtyas, Tri Rini; Purnomo, Purnomo; Rachmawati, Diah; Putra, Eka Tarwaca Susila; Utomo, Condro; Liwang, Tony; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109941

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.
Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant Sasongko, Aries Bagus; Purwestri, Yekti Asih; Daryono, Budi Setiadi; Subandiyah, Siti
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111727

Abstract

The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213). This study evaluates the endophytic bacterium AP3311, isolated from healthy banana roots in direct association with orchid roots. AP3311 exhibited strong antagonism toward FocTR4, hyphal colonization ability, and multiple growth‐promoting activities, including phosphate solubilization, nitrogen fixation and auxin production. 16S rRNA gene sequencing identified that AP3311 belongs to the genus Bacillus, while metabarcoding analysis revealed that Bacillus species dominate the root microbiomes of both bananas and orchids. The bacterial supernatants stimulated root development and leaf growth in vitro. Metabolomic profiling indicated that antimicrobial compounds, together with plant growth regulators, promoted both root and shoot growth. Overall, the research demonstrates that Bacillus sp. AP3311 and its supernatants are valuable components in banana tissue culture, providing the dual benefits of plant growth promotion and effective disease control.
CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition Yuanita Rachmawati; Ganies Rizaa Aristya; Budi Setiadi Daryono
Biotropic : The Journal of Tropical Biology Vol. 1 No. 2 (2017): Biotropic, Volume 1, Nomor 2, 2017
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2017.1.2.1-8

Abstract

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.
Phenotipical Characters of Melon (Cucumis melo L.) in Response to Karst Critical Land Yuanita Rachmawati; Budi Setiadi Daryono; Ganies Riza Ariestya
Biotropic : The Journal of Tropical Biology Vol. 2 No. 1 (2018): Biotropic, Volume 2, Nomor 1, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.1.1-10

Abstract

Yogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference.
KARAKTER FENOTIP KEDELAI (Glycine max (L.) Merr.) HASIL POLIPLOIDISASI DENGAN KOLKISIN Irma Nofitahesti; Budi Setiadi Daryono
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 5 No. 2 (2016)
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v5i2.957

Abstract

Abstract - Soybean (Glycine max (L.) Merr) is one of the most important food commodity to fulfill the protein necessity in Indonesia. Although Indonesia has many prime soybean seeds, it cannot fulfill the whole need of soybean and always rely on soybean import. This problem can be solved by increasing the quality and productivity of prime soybean seed by polyploidization with colchicine. This research aimed to study ploidy level and phenotype characters of Anjasmoro soybean which was induced by colchicine. The phenotype characters in this research were stomata size, plant height, total pod in one plant, total seed in one plant, weight of 100 seeds, flowering time, and ripening time of soybean. The ploidy level was analyzed with flow cytometry methode. The data was analyzed with one way ANOVA and Duncan test in SPSS 22 program. The result of this research showed that Anjasmoro soybean did not successfully induced by colchicine using concentration 0.01%, 0.02%, 0.025%, 0.05%, 0.075%, 0.1%, 0.15%, 0.2%, and 0.25% with duration of treatment 6, 8, 10, 12, 16, 18, and 24 hours. The treatment with colchicine concentration 0.01% and 0.02% with duration of treatment 10 hours effected the increasing of stomata size, the plant height, and the weight of 100 seeds.Key words : Soybean, polyploidization, colchicine, phenotypeAbstrak - Kedelai (Glycine max (L.) Merr) merupakan salah satu komoditas pangan penting sebagai sumber protein nabati yang kebutuhannya selalu mengalami peningkatan di Indonesia. Meskipun saat ini Indonesia memiliki banyak varietas kedelai unggul, namun Indonesia masih belum mampu mencukupi kebutuhan kedelai nasional sehingga terus bergantung pada impor kedelai. Permasalahan ini dapat diatasi dengan meningkatkan kualitas dan produktivitas varietas kedelai unggul yang sudah ada melalui teknik poliploidisasi dengan kolkisin. Penelitian ini bertujuan untuk mengetahui derajat ploidi dan karakter fenotip pada kedelai Anjasmoro yang diinduksi dengan kolkisin. Karakter fenotip yang diamati adalah panjang dan lebar stomata, tinggi tanaman, jumlah polong per tanaman, jumlah biji per tanaman, berat 100 biji, umur berbunga, dan umur masak kedelai. Analisis derajat ploidi kedelai dilakukan dengan metode flow cytometry. Data yang diperoleh dianalisis dengan uji statistik one way ANOVA dan dilanjutkan dengan uji Duncan taraf 5% menggunakan program SPSS 22. Hasil penelitian menunjukkan bahwa kedelai Anjasmoro belum berhasil terinduksi poliploid dengan perlakuan kolkisin konsentrasi 0,01%, 0,02%, 0,025%, 0,05%, 0,075%, 0,1%, 0,15%, 0,2%, dan 0,25% dengan lama waktu perlakuan 6, 8, 10, 12, 16, 18, dan 24 jam. Perlakuan kolkisin konsentrasi 0,01% dan 0,02% dengan lama waktu perlakuan 10 jam mempengaruhi peningkatan ukuran stomata, tinggi tanaman, dan berat 100 biji kedelai Anjasmoro.Kata kunci : Kedelai, poliploidisasi, kolkisin, fenotip
Aplikasi Teknologi Budidaya Kelengkeng Super Sleman di Padukuhan Gejayan DARYONO, BUDI SETIADI; RABBANI, ANNAS; PURNOMO, PURNOMO
Bioedukasi: Jurnal Pendidikan Biologi Vol 9, No 1 (2016): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v9i1.3890

Abstract

Longan (Dimocarpus longan L.) is not an indigenous fruit from Indonesia. Thus, the demands for this fruit were supplied by importing them from nearby countries such as Vietnam, Thailand, and China. Longan has been cultivated and developed in Indonesia in order to supply the local needs and to reduce the rapid flows of importing Longan. Recently, a brand new breed of local Longan has been engineered from Sleman District of D.I.Yogyakarta Province, known as Kelengkeng Super Sleman (KSS). The KSS well adapted to medium-high above sea level- terrain, the most typical terrain in Sleman. Some prosperous features of this Longan cultivar were had a short harvesting time (2 years), thicky flesh, low water content, and able to be induced twice per year for flowering (to produce fruits). All of this goodness thus been introduced to rural communities of Padukuhan Gejayan, Desa Condongcatur, Depok, Sleman, D.I.Yogyakarta by a well designed community service programme. The kind of activites that was going on in this programme were workshop, training, and assistance. Workshop was emphasized on the prospectous consequences of planting Longan trees (KSS) for a long term until could be developed into local industrial product (One Village One Product/OVOP concept), basic knowledge on Longan diversity, KSS cultivation technology, basic knowledge on plant physiology, environmental development based on Education for Sustainable Development (EfSD), and potential local market for KSS. Training was mainly focused on KSS seedling production by both apical stem grafting method and medium preparation method, KSS plantation in some villager houses for a pilot project on how to maintain KSS such as health evaluation, health recovery for infested or unhealthy plant, method on lateral branching development, method on hormone-induced flowering, and application of resting period for after-harvesting plant. Assistance has been actively conducted during training to motivates villager to be get involved. Some positive indicators for implementation of this programme are both the great enthusiasm on KSS Longan plantation in communities and “the shifting paradigm” on KSS Longan maintenance for which has been long considered to be both impractical and arduous.
Sifat Fungsional dan Tingkat Penerimaan Masyarakat Atas Produk Olahan Labu Susu (Cucurbita moscatta ‘Butternut’) sebagai Upaya Pencegahan Anemia dan Stunting Nurpuji Mumpuni; Yuli Astuti; Dewi Zolekhah; Budi Setiadi Daryono; Supriyadi Supriyadi; Lily Arsanti Lestari
The Journal of Innovation in Community Empowerment Vol 7 No 2 (2025): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v7i2.1953

Abstract

Anemia and stunting remain a major challenge in public health issues, especially in pregnant women and toddlers. Cucurbita moscatta ‘Butternut’ (Labu susu), which is rich in essential micronutrients uch as Beta Carotene (provitamin A), folic acid, iron, and zinc, can be processed into highly potent functional foods. Aside from being an excellent source of alternative carbohydrates, it can also meet the micronutrient needs to support the prevention of Anemia and stunting. This activity was carried out at the Community-Friendly Green Open Space (RTH - Ruang Terbuka Hijau) in the Bener sub-district, Tegalrejo district, Yogyakarta City, with the aim of analyzing the taste acceptability of processed bottle gourd products among the target groups. The implementation methods included socialization, taste testing, and acceptability analysis of three processed products based on Bottle Gourd: Nutrient-Rich Baby Porridge, High-Protein Mud Cake (Kue Lumpur), and Compote for Pregnant Women (Kolak Ibu Hamil). The activity involved 50 panelists who assessed the color, aroma, taste, texture, and overall acceptance of the three processed products using a six-level hedonic scale. The results showed that all three products were well-received. For all indicators ( Color, Aroma, Taste, and Texture), all products received scores above 4.6, and the overall acceptance was above 5. The overall acceptance score for the High-Protein Mud Cake (5.22 ± 0.6), the Kolak for Pregnant Women (5.18 ± 0.7), and Nutrient-Rich Baby Porridge (5.00 ± 0.8). The conclusion of this research is that panellists “like” and “accept / receive” all three products. This activity had a positive impact on increasing public knowledge regarding the diversification of local food and served as a strategic rocessed products; step in the effort to prevent anemia and stunting at the family level through the fulfillment of essential micronutrients.
Uji Ketahanan Tujuh Kultivar Jagung (Zea mays L.) Terhadap Penyakit Bulai (Peronosclerospora spp.) Daryono, Budi Setiadi; Parazulfa, Anisa; Purnomo, Purnomo
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 1 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i1.4175

Abstract

Maize (Zea mays L.) is one of important food in Indonesia. Java Downy Mildew is a plant disease caused by Peronosclerospora spp. This specific fungus attacks corn plants, especially the leaves. The purpose of this study was to examine maize cultivars resistance to Java Downy Mildew.  This research was conducted at the land owned by Agrotechnology Innovation Center (PIAT) UGM located in Kalitirto Village, Berbah Subdistrict, Sleman Regency, Special Region of Yogyakarta. The main ingredients used in this study included maize with 7 different cultivars: Gama GS, Gama SG, Bisi 816, Pioneer 21, Lagaligo, Talenta, and Pulut. This research was conducted using Randomized Block Design with 7 treatments and 2 replications. The results showed that Talenta, Lagaligo and Bisi-816 were resistant to downy mildew. Pioneer-21 quite resistant to downy mildew. Cultivars Gama GS, Gama SG and Pulut were not resistant to downy mildew. This study provides new information related to the infection of downy mildew especially in Yogyakarta.
Inheritance and Comparison of Phenotypic Characters from Hybrid Chicken GK-Bro (Gallus gallus Linnaeus, 1758) Damayanti, Putu Ayu; Daryono, Budi Setiadi; Mahardhika, I Wayan Swarautama
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.9493

Abstract

GK-Bro (Gallus gallus Linnaeus, 1758) is a hybrid chicken from crossing between ♀ F2 Broiler ♂ F2 Golden Kamper that have variation of phenotypic character. To meet the market’s demand, GK-Bro must have prime phenotypic character in both quantity and quality. This research aims to examine the phenotype character that occur in GK-Bro by the character of feet morphometry, crest’s shape, feather’s color, and feet’s color. The parental chicken are crossed in Innovation and Agriculture Centre of Gadjah Mada University (Indonesia) and produce 11 chickens with five males and six females filial. The filial are maintained intensively and qualitative data were observed at the end of seven weeks, classified based on the percentage of the phenotypic class. Quantitative characters that observed were tibia and femur length after seven weeks, using control pelung, broiler, and F1 broiler. Quantitative data showed that the average length of the tibia and femur of GK-Bro is lower when compared to the control but the ratio of feet morphometry is closer to pelung. Qualitative data classified crest form in one class, feather’s color in 4 classes and feet’s color in 3 class. 
Morphological Traits, Productive Performance and Genotyping Fat Deposition PPAR Gene in Gama Ayam Crossbreeds of Female F1 Kamper and Male BC1 Kambro Ummah, Isna Mustafiatul; Mahardhika, I Wayan Swarautama; Daryono, Budi Setiadi
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.9993

Abstract

On the recent classification of native Indonesian chicken, there are 31 breeds of chicken. Highly circulated chicken breed in Indonesia is fast-growing broiler that includes several strains such as Cobb 500, Hubbard, Hybro, Isa Hyline, and Hisex. Fast-growing broiler chickens have rapid growth, with excessive fat deposition on chicken carcass which implicates a health problem and unfavorable meat quality. Gama Ayam Research Team conducted selective breeding which produces two new chicken breed Kamper and Kambro. Further selective breeding then crossbred female F1 Kamper and male BC1 (backcross I) Kambro. In this research morphological traits, productive performance and genotyping of PPAR gene related to fat deposition gene and blood lipid content in Gama Ayam were identified. Based on data analysis hybrid chickens morphological traits it concluded that Gama Ayam has a significant variation based on feather color. The productive performance was determined with the feed-conversion ratio (FCR) value which was 3.17. Genotyping of PPAR gene resulted in four polymorphisms that formed 14 haplotypes groups. Based on blood lipid content analysis of cholesterol content, triglycerides content, HDL and LDL, Gama Ayam have significantly lower content of cholesterol (107.05 mg/dl), LDL (44.71 mg/dl) and triglycerides (22.41 mg/dl). PPAR gene polymorphisms are not correlated with blood lipid content in Gama Ayam. A significantly strong correlation between PPAR gene polymorphism on the bodyweight of Gama Ayam at 49-days-old. Triglyceride level, cholesterol level and LDL level in Gama Ayam were lower than broiler chicken. Further research with a larger population size and sex classification of hybrid chicken must be conducted to validate the results.
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Aries Bagus Sasongko, Aries Bagus Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Budy Wiryawan Catur Ahda Darojatun Damayanti, Putu Ayu Darmastuti Deris Trian Rahmandhias Desi Oktaviani Diah Rachmawati Diah Rachmawati DIAH RACHMAWATI DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Tarwaca Susila Putra Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol Husnun, Fadilah I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Irma Nofitahesti Ishak, Muhammad Alif Ismail, Ismail Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kurniawan, Febri Yuda Kusnanda, Prima Sekti Kyswantoro, Yunita Firdha Latifah, Vida Rahma Lily Arsanti Lestari Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahardhika, I Wayan Swarautama Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Mumpuni, Nurpuji Narzassi, Angga Bintang Nia Fararid Askar Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurdilfa, Nurdilfa Ovami, Debbi Chyntia Pabendon , Marcia Bunga Parazulfa, Anisa Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putro, Karso Suryo RABBANI, ANNAS Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ramlah Ramlah Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Roberdi, Roberdi Rohman, Randi Abdur Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Siti Subandiyah Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, ‪Teuku Nanda Saifullah Sulaiman, ‪Teuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaiman‬, ‪Teuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supadmi, Francisca Romana Sri Supriyadi Supriyadi Susamto Somowiyarjo Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tony Liwang Tri Rini Nuringtyas Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Ummah, Isna Mustafiatul Utin Elsya Puspita Utin Elsya Puspita Utomo, Condro Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yekti Asih Purwestri Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yuli Astuti Yuliara, I Made Yusuf, Adib Fakhruddin Zolekhah, Dewi