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Hendra Susanto

Universitas Negeri Malang

Author-ID : 2685407

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Novel Self-Nanoemulsifying Drug Delivery System of Single Bulb Garlic: Stability, Toxicity, and Antiinflammation in 3T3-L1 Cells Dewi Sekar Miasih; Yuslinda Annisa; Sri Rahayu Lestari; Hendra Susanto; Sunaryono
Science and Technology Indonesia Vol. 7 No. 4 (2022): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Single bulb garlic (SBG) has the potential as an immunomodulator; however, it has low solubility and bioavailability. A lipid-based delivery system, that is, the self-nanoemulsifying drug delivery system (SNEDDS), offers a novel opportunity in drug delivery. SBG can be a suitable candidate for SNEDDS development. This research aims to describe the physical stability, toxicity test of the SNEDDS, and SNEDDS SBG potential as antiinflammation in 3T3-L1 cells. The SNEDDS is made with various ratios of concentrations of carrier oil, surfactants, and cosurfactants, namely, 0.50: 3.45: 0.96, and added with SBG extract (SBGE) of 20 mg/mL. The results of the response test of SNEDDS SBGE formulation indicate an average and standard deviation of emulsification time of 16.38±3.01 (second), pH 7.21±0.08, and transmittance of 98.40±0.23(%). The average nanoemulsion size is 14.333±0.416 nm, polydispersion index of 0.213±0.056, and zeta potential of -14.67±0.72 mV. The results of the physical stability test indicate no segregation, deposition, cracking, or creaming in all nanoemulsion samples and SNEDDS SBG. The MTT test in a dose of 62.5, 125, 250, 500, 100, 2000, and 4000 µg/mL suggests that the highest viability of the 3T3-L1 cells is at a dose of 2000 µg/mL, which is 97.83%±1.55%. Therefore, SNEDDS SBGE can be a potential candidate for oral preparation by increasing bioavailability and reducing toxicity in the 3T3-L1 cells. An antiinflammatory test on the TNF-???? and IL-1???? expressions influences the 3T3-L1 cells. the SNEDDS of SBGE has the potential to reduce the expression of TNF-???? and IL-1???? and increase IL-10 expression in the methylglyoxal-induced 3T3-L1 cells.

Mutation Analysis and Single Nucleotide Polymorphism of TP53 Gene in Breast Cancer in East Java Rizqa Radhiyah; I Kade Karisma Gita Ardana; Wisnubroto Wisnubroto; Dwi Listyorini; Hendra Susanto
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.12

The Incidence of Indonesia breast cancer case in 2018 was reported at 20.7% or 160,653 in number. The factors that caused breast cancer is TP53 gene mutation and Single Nucleotide Polymorphism (SNP). This study aimed to determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53 gene in breast cancer. Samples of this study were 9 people chosen based on a purposive technique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencing were then analyzed using alignment and blast. The SNP is browsed by the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A) is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exon and altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain. Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also contribute to the development of cancer stage. In addition, Single Nucleotide Polymorphism in the TP53 gene can be used as a predisposition marker of breast cancer that has potential to be inherited

Potential of Single Garlic to Prevent Pro Inflammatory Macrophage and Inflammation in HFD Mice Putri Diyah Anggraini; Miftahul Mufinadiroh; Hendra Susanto; Betty Lukiati; Sri Rahayu Lestari
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.10

A high-fat-enriched diet causes an increase in the level of oxidized LDL (Ox-LDL) in the blood. The presence of Ox-LDL will activate macrophages to secrete pro-inflammatory cytokines and lead to severe inflammation. Single bulb garlic has a potential anti-inflammatory effect due to of high-fat diet. This research aimed to investigate the effect of single bulb garlic extract (SBGE) on the pro-inflammatory cytokines TNF-Î± (CD11b+TNF-Î±+) and IL-1b (CD11b+IL-1b+) in the spleen, spleen weight, and TNF-Î± secretion in HFD mice. Twenty-four mice were divided into six groups: normal (healthy mice); HFD (HFD mice without any treatment); HFD + Simvastatin (HFD mice receiving simvastatin); HFD + SBGE 100; HFD + SBGE 200; and HFD + SBGE 400 (HFD mice receiving 100, 200, and 400 mg/kg BW of SBGE for 4 weeks). Blood serum was collected at the end of treatment, and macrophage was isolated from the spleen. The relative number of CD11b+TNF-Î±+ and CD11b+IL-1b+ were examined using flow cytometry. SBGE treatment significantly (p<0.05) reduced the spleen weight and the relative number of CD11b+TNF-Î±+ and CD11b+IL-1b+ in the spleen of HFD mice. SBGE treatment also prevents the elevation of TNF- Î± levels in the blood serum. The optimal dose of SBGE to diminish the relative number of CD11b+TNF-Î±+, CD11b+IL-1b+ in the spleen, and TNF-Î± in the serum was 100 mg/kg BW.

Differences of alt-ast level and snail gene expression between patients with metastasis and non-metastasis colorectal cancer Chrisna Budi Satriyo; Hendra Susanto; Adeodatus Yuda Handaya; Nurcahya Setiawan; Imam Sofii; Agus Barmawi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 13, No 3, (2022)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol13.Iss3.art5

Background: Colorectal cancer (CRC) is a malignant epithelial tumour in the colon and rectum, and its main cause is distant metastasis. The main process of distant metastasis is the epithelial-mesenchymal transition (EMT) that may cause mesenchymal phenotype and malignant capabilities of tumour cells. Then it is regulated by one of transcription factors, the Snail.Objective: This study aims to determine differences between Snail gene expression in CRC that has not metastasized and Snail gene expression in CRC that has metastasized. Methods: The subjects of this study consisted of two groups of patients. The first group consisted of 20 patients with CRC (tumour), and the second group consisted of 12 patients with CRC with metastasis (metastasis tumour). The Snail gene expression was measured in both groups by using the RT-qPCR method and supported by laboratory examination to measure ALT and AST. Then all obtained data were analysed by using a statistical software analysis.Results: This study indicated that there were differences of the Snail gene expression between both the groups, and the highest expression was in the metastatic tumour group with a significance level of 0.002 (p<0.05). This was also supported by AST and ALT which were significantly correlated between both the groups. The correlation value on the AST was 0.506 with a significance level of 0.003, and the ALT correlation value was 0.532 with a significance level of 0.002. Conclusion: This study suggested that the Snail was a potential marker of metastasis in the CRC. Therefore, further research is needed to determine the role of Snail regulation in the CRC metastasis.

Definition and conceptual model of genetics literacy: a systematic literature review Hidayati Maghfiroh; Siti Zubaidah; Susriyati Mahanal; Hendra Susanto
International Journal of Public Health Science (IJPHS) Vol 12, No 2: June 2023
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v12i2.22679

Genetic literacy is essential for promoting health and well-being in modern society. Although its importance is increasingly recognised, the definition and crucial dimension to construct a conceptual model are unclear, limiting the possibilities for measurement and comparison intervention. The study aims to review definitions and the conceptual models of genetics literacy and develop a new comprehensive definition and conceptual model based on the discovered dimensions that are relevant in the post-genomic era. We performed a systematic literature review using the Crossref, PubMed, and Scopus databases with Publish or Perish software. An automated content analysis was conducted to identify and develop conceptual model genetics literacy using NVivo 12 software. The review resulted in 10 definitions and 12 conceptual models. Automated content analysis showed that genetic literacy is defined as the ability of an individual to comprehend, use, correlate, assess, and propose genetic information to make arguments, reason, and decide on genetic issues in maintaining or improving the quality of personal and social well-being. Genetic literacy was conceptualised as a set of knowledge, a set of skills or interconnected. The conceptual model of genetics literacy covers two dimensions. i) Knowledge dimension: conceptual (nature of genetic material, transmission, genetic expression, genetic regulation, genetic determinism, genetic technology); sociocultural; epistemic. ii) Skills dimension: argumentation, informal reasoning, and decision-making skills. This definition and conceptual model can serve as a basis for developing interventions and measurements to support regulating, preventing, as well as promoting health and well-being.

Pemanfaatan Teknologi Sel Surya untuk Optimalisasi Budidaya Ikan Lele Berbasis Sistem Biofloc-Aquaponic Sunaryono Sunaryono; Markus Diantoro; Ahmad Taufiq; Hendra Susanto; Muhammad Ikhwan Najmi; Futri Yuliana
JPM: Jurnal Pengabdian Masyarakat Vol 5 No 1 (2023): Dedikasi: Jurnal Pengabdian Masyarakat, Januari-Juni 2023
Publisher : Pusat Pengabdian Masyarakat (PPM) Lembaga Penelitian dan Pengabdian Masyarakat (LP2M) Institut Agama Islam Negeri (IAIN) Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32332/d.v5i1.5684

Gadingkasri Village is one of the villages located in the Klojen District of Malang City which is located in the downtown area of Malang. The location of the village surrounded by a business center and several universities makes this village very strategic because it is a densely populated residential area with a combination of residential communities and students as boarding house residents. With this condition, Gadingkasri Village has considerable economic zone potential for its people. Gadingkasri Village, especially residents in RW. 3, there is a community of PKK mothers who are members of the Urban Farming Group who have a vision to green residential areas by gardening around settlements. One of the potentials that can be developed is to develop community activities in the Urban Farming community towards optimizing energy and increasing residents' income by raising catfish. The community service program through funding from the State University of Malang aims to maximize the existing potential through the use of appropriate technology. The method used in this service is a method of empowering catfish farming by utilizing appropriate technology engineering for sustainable alternative energy. The activities that have been carried out are in the form of mentoring, designing, installing aquaponic systems, and biofloc pools, and solar panels which will be alternative energy in supplying aquaponic pump electrical systems and biofloc ponds. The results of this service activity are expected to be an alternative energy source solution that can be used independently by RW residents. 3 Gadingkasri village. The empowerment of catfish with a biofloc system is also expected to be able to increase the economic income of residents

The Development Project Based-Learning Food Microbiology E-Module Based on The Study of Kombucha Fermentation Lianto Lianto; Sri Rahayu Lestari; Hendra Susanto
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 8, No 5: MAY 2023
Publisher : Graduate School of Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/jptpp.v8i5.24401

This research is research and development based on Lee and Owens’ design that aims to develop a valid and practical food microbiology electronic module based on Project-Based Learning (PjBL). The research and development consisted of five steps: analysis, design, development, implementation, and evaluation. The validity of e-module was assessed by content expert, media and teaching material expert, and biology education practitioner. The practicality of e-module was assessed by The State University of Malang students who had joined food microbiology course. The instruments of this research were validation sheets of content expert, media and teaching material expert, biology education practitioners, and student questionnaire sheet responses. The development outcome showed that the validation of the content experts, media and teaching, and biology education practitioner give a percentage of 100%. The outcome of the practicality of e-module showed that the individual trial gets a percentage of 92,44%, small group is 90,96%, and the field is 92,96%. According to the outcomes, it can be inferred that e-module have filled the validity and practicality necessity to be applied as teaching material in food microbiology courses.

Detecting miRNAs expression as the early prognostic factor for patients with colorectal cancer in Dr. Sardjito Hospital Yogyakarta : A preliminary study Adeodatus Yuda Handaya; Didik Setyo Heriyanto; Hendra Susanto; Yudi Susanto; Kamal Agung Yudayana; Ida Ayu Setyawati Sri Krisna Dewi; Aditya Rifqi Fauzi; Joshua Andrew; Kevin Radinal; Azriel Farrel Kresna Aditya
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 14, No 2, (2023)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol14.Iss2.art9

Background: Colorectal cancer (CRC) is the third highest-ranked cancer and causes high mortality in patients with a low survival rate. The lack of sensitivity and specificity of clinical and other diagnostic modalities results in a higher mortality rate. Therefore, the exploration of potential early biomarkers for CRCs is necessary.Objective: We aimed to evaluate the local expressions of potential tumor suppressor and oncogenic miRNAs in CRC patients in Indonesia. Methods: This retrospective cohort study involving thirty-one colorectal carcinoma patients at Dr. Sardjito Hospital Yogyakarta from January 2014-December 2017. Total RNA was isolated, and the expressions of miR-21, miR-92a, miR-96, miR-26b, miR144, and miR-195 were measured by real-time quantitative PCR. The correlation between miRNAs and other predictors was determined by Spearman correlation, and the association of miRNA expression and other clinical parameters used logistic regression.Results: The local expression of miR-195 decreased significantly in the tumor sites. In contrast, miR-21 activity tends to increase in the local tumor. Meanwhile, the expressions for miR-92a, miR-96, miR26b, and miR-144 in the same subjects were non-significant. MiR-195 was also significantly associated to cancer stage (r=-0.570, p=0.001) with significant odds ratio (OR=0.892, 95% CI=0.804–0.990, p=0.031).Conclusion: Our study was the first to report aberrant expressions of miRNA-21, miRNA-195, miRNA-92a, miRNA-26b, miRNA-96, and miRNA-144 in Indonesian CRC patients. The tumor suppressor miRNA-195 expression was superior among others to serve as an early biomarker in detecting and predicting CRC disease progression.

The Development of Electronic Modules Based on Problem Based Learning Integrating Disaster Mitigation in Environmental Change Material to Increase Critical Thinking Skills of Students Sinta Kusuma Dewi; Hadi Suwono; Hendra Susanto
BIOEDUKASI Vol 21 No. 3 (2023)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v21i3.41064

An e-module is a learning media designed to assist students in discovering and solving problems independently and can be used practically and flexibly. The purpose of this paper is to produce a PBL-based integrated disaster mitigation e-module on environmental change material that is valid, practical and effective. The e-module development used the development model by (Lee & Owens, 2004). E-modules were validated by material experts, learning media experts, biology education experts and practitioners. Furthermore, the e-modules were tested on students in individual, small group and field trials. In the field trial, the e-module was applied in biology learning. Based on the N-Gain test score between pretest and posttest, it is known that the experimental class is in the high category and the control class is in the medium-high category. The results prove that the e-module developed is valid, practical and effective, so it can be used in learning activities that can improve students' critical thinking skills.

Revolutionary Biology Education: Development of Advanced Biology Learning Through Websites and Learning Kits Hendra Susanto; Ibrohim; Andi Basuki; Wiedia Carullina Purwanti; Ari Gunawan; Afis Baghiz Syafruddin
International Journal of Science and Society Vol 5 No 5 (2023): International Journal of Science and Society (IJSOC)
Publisher : GoAcademica Research & Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54783/ijsoc.v5i5.882

Biology education has become essential in developing students' scientific understanding and skills in facing global challenges. Advanced biology learning can be introduced to students as an effort to apply science in everyday life and innovative research. This field of biology has opportunities for promising career paths and jobs in science and technology. However, this learning has yet to be widely introduced regarding innovations in the field of biology in various aspects so that it can deepen students' understanding and competence in solving problems. This research aimed to develop websites and learning kits in advanced biology education. The method used was ASSURE, including analysis of student characteristics, setting performance targets, media development, material exploration, evaluation, and revision. The results obtained in the research, covering the validation analysis of biological material, had met the aspects of depth, feasibility, clarity, suitability for purpose, and use of language. Validation analysis of video-based learning media had fulfilled the aspects of video quality, audio clarity, content suitability, text readability, and context connectedness. The learning platform validation analysis had met user experience, content availability, and visual quality. Recommendations for further research include a validation process by experts and field implementation to determine the effectiveness of learning media.

Co-Authors Achmada, Putra Fernanda Dwi Adeodatus Yuda Handaya Adim Firmansah Adinda Marcelliantika Aditya Rifqi Fauzi Afis Baghiz Syafruddin Agung Witjoro Agus Barmawi Agus Subagyo Agusta Rakhmat Taufani Agustina, Rena Silvia Ahmad Hapiz Ahmad Khanali Ahmad Taufiq Ahmad Taufiq Ainun Nikmah Ajeng Daniarsih Alby Aruna Aldri Frinaldi Ali Mustofa Almas Dwi Khairana Amaliyah Firdaus, Sa’diyatul Rizqie Ananda, Oriny Tri Anastya Eka Kharisma Andi Basuki Anggarani, Dinar Arsy Anggarani, Dinar Arsy Arsy Annisa, Yuslinda Arbin Janu Setiyowati, Arbin Ardana, I Kade Karisma Gita Ari Gunawan Ari Gunawan Ari Gunawan Arif Hidayat ARIF HIDAYAT Aripriharta - Arisatya Bharotoyakti Aruna, Alby Aulanni'am, Aulanni'am Aulanni’am, Aulanni’am Azriel Farrel Kresna Aditya Bagus Priambodo Bahrullah Akbar Betty Lukiati cahya, uci dwi Chang, Chun-Yen Choirunisa', Nindiana Chrisna Budi Satriyo Chusna, Nadiya Miftachul Claresia Tsany Kusmayadi Deny Setiawan Deny Setiawan Dewi Murni Dewi Sekar Miasih Dhaniswara K. Harjono Diah Setiawati Didik Dwi Prastyo Didik Setyo Heriyanto Dwi Listyorini Eka Putri Surya El Baroroh, Alif Rosyidah Facthur Rohman Fatchur Rohman Fatchur Rohman Faturrahman Fauzi, M Imam Friska, Renny Futri Yuliana Fuzani, Sri Dinasyah Ratna Hadi Suwono Hadi, Sholihul HARDIANTO - Hari Rudijanto. IW Heru Wahyu Herwanto Hidayati Maghfiroh Hulman Panjaitan, Hulman Husna Nur Wanah I Kade Karisma Gita Ardana I Made Wirawan Ibrohim Ida Ayu Setyawati Sri Krisna Dewi Ida Rohmah Susiani Ika Sasti Ferina, Ika Sasti Ikramina Yusti Amina Imam Sofii Indah Nur Laili Isma, Rusdatul Izza, Jasmine Nurul Janiar Pitulima Jasmine Nurul Izza Joshua Andrew Judhiastuty Februhartanty Kamal Agung Yudayana Kamal, Abu Hena Mostofa Kevin Radinal Kormil Saputra Krisnawati, Dyah Ika Kristianty, Grace Triana Kusmahardhika, Noviansyah Kusmayadi, Claresia Tsany Kusnunnahari Kusnunnahari, Kusnunnahari Kustiariyah Tarman Lianto Lianto Linggasari, M.I. Dewi Lulik Inggarwati Lusttilanang, Pius Lya Rizka Herawati M Iqbal Najib Fahmi M. Ikhsan M. Rasjad Indra Maghfiroh, Hidayati Mariya Sulfa, Devi Markus Diantoro Marshanda Putri, Anggia Marwan Marwan Masruroh MASRUROH Mega Safithri Miasih, Dewi Sekar Miftahul Hasanah Miftahul Mufinadiroh Mimien Henie Irawati Moch Sasmito Djati Moch Sholeh Mochammad Imron Awalludin Moh. Zainul Falah Mohamad Amin Mohammed, Najib Muhammad Adib Amin Muhammad Rifqi Hariri Muhammad Saefi Muharni, Andi Murni Sapta Sari Muslihati Najmi, M. Ikhwan Nik Ahmad Nizam Nik Malek Nik Malek, Nik Ahmad Nizam Nurcahya Setiawan Nurlita Indah Wahyuni, Nurlita Indah Nurul Hidayat Nurul Izza, Jasmine Oktavia, Silvi Tri Oriny Tri Ananda Paidi Paidi Pramono, Agus Joko Prayogi, Moch. Bagus Putri Diyah Anggraini Putri, Kuntum Kinanthi Rachmawati , Hidajah Radhiyah, Rizqa Rajib Muhammad Basthony Rasjad Indra Ratnapuri, Adisti Rawe, Nining Sahara H A Restianto, Yanuar Retna Apsari Ricadonna Raissa Rizqa Radhiyah Robi, Fikri Syahir Rony Irawanto Rui Alfadel Saputra S, Muh Akbar Sabrina, Andiani Salsa Salim, Fitrah Amalia Satia Nur Maharani Satria Adiguna Seha, Siti Nur Setyarini, Ni Luh Putu Shinta Setyohadi, Taat Sholeh , Moch sholeh, moch Sholihah, Nida Anisatus Sholihul Hadi Sinta Kusuma Dewi Siti Maisaroh Siti Zubaidah Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Soenar Soekopitojo Soerjadjanegara, Moermahadi Sri Endah Indriwati Sri Rahayu Lestari Sri Rahmawati ST. Ulfawanti Intan Subadra Subadra, ST. Ulfawanti Intan Sudjari Sudjari Sudjari Sudjari Suharti Sujito . Sukiman . Sulfa, Devi Mariya Sunaryono Sunaryono Sunaryono, Sunaryono Supratman Tabba Susriyati Mahanal Suwarno Suwarno Suwistika, Rima Sya'rawi, Muhammad Akram Syalwa, Mutiara Tjahyono, Seto Tono, E.P.S.B Taman Tri Endharti, Agustina Trijoyo Purnomo, Jerry Dwi Umi Fitriyati Utami Sri Hastuti Verdamil, Ainun Cornelia Wahyu Pratama, Ade Wandreina, Lucky Meisya Wibi Riawan Wiedia Carullina Purwanti Wijiasih, Wijiasih Wira Eka Putra Wisnubroto Wisnubroto Wisnubroto, Wisnubroto Wiwin Apriani Wuragil, Dyah Kinasih Yuda Handaya, Adeodatus Yudi Susanto YULIA SAFTIANA, YULIA Yuliana, Futri Yunita Rakhmawati Yuslinda Annisa Zahra Firdaus

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