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PREVALENCE OF TREMATODES IN JAVAN RHINOCROS AND BANTENG IN UJUNG KULON NATIONAL PARK Risa Tiuria; Jimmy Pangihutan; Ripta Mustafa Nugraha; Bambang Pontjo Priosoeryanto; Adhi Rahmat Hariyadi
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Javan rhinoceros (Rhinoceros sondaicus) and Javan bull (Bos javanicus) in National Park Ujung Kulon are protected wild animals. A study to examine the presence of trematode eggs in their fecal samples was carried out. The fecal samples were collected based on the transec route in National Park Ujung Kulon. The presence of eggs in fecal samples was examined by filtration method. Trematode eggs were found in 56% of Javan rhinoceros fecal samples and 73,91% of Javan bull fecal samples.. Base on the size of the eggs it was determined that Javan bulls in National Park of Ujung Kulon were infected by Fasciola spp (17,39%) and Paramphistomum spp (56,52%). Javan rhinoceros in National Park of Ujung Kulon was infected with Fasciola spp (44%) and Schistosoma spp (12%). This study clearly showed that tramatode infection is commom among wild ruminants in Ujung Kulon National Park and attention should be taken to improve the health status of the animals especially Javanese rhinoceros and Javanese bull
Tepung Tempe Kaya Isoflavon Meningkatkan Kadar Kalsium, Posfor dan Estrogen Plasma Tikus Betina Normal I Nyoman Suarsana; I Nyoman Sadra Dharmawan; I Wayan Gorda; Bambang Pontjo Priosoeryanto4
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to study the effect of isoflavon-riched tempe flour on calcium (Ca, phosphate(P), and estrogen levels in plasma et normal female rats during their growth period. A level oftwenty-five 2 months old female Sprague Dawley rats with an avarage body weight of 200 g wasrandomly divided into into 5 groups: one with control group (KO: without treatment) and fourtreatment groups (K1, K2, K3, K4 : animals were given tempe flour with isoflavon at 1; 2; 4; and 6 mg/200 g/bw, respectively). The treatment was conducted for two months, following this blood plasmawas collected to analyse the level of calcium, phosphor, and estrogen, respectively. The resultshowed that although the plasma level of Ca, P, and estrogen was higher in the treatment groupcompare to the control group, this was not significantly different (P>0,05). The highest plasmalevel of Ca, P, and estrogen was seen in anmal receiving tempe flour with 4 mg/ 200 g bw/dayisoflavon.
Antiproliferation activity of water and ethyl acetate red yeast rice fraction against MCM-B2 tumor cells Hasim Hasim; Surya Pangidoan Nasution; Bambang Pontjo Priosoeryanto; Eka Nurul Qomaliyah
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.395 KB) | DOI: 10.12928/pharmaciana.v10i3.16951

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Red yeast rice (RYR), commonly known as angkak, is a functional food fermented by rice using Monascus mushrooms. It has a pigment and flavonoid content that is useful as an antioxidant and plays a role in preventing cancer or tumors. This analysis aims to test the antiproliferation activity of commercial RYR fractions of water and ethyl acetate against Miyazaki Canine Mammary Gland Tumor-Bambang 2 (MCM-B2) breast tumor cells. Research on RYR antiproliferation of cancer-sustaining MCM-B2 cells has not been previously released. In the preliminary study, the antiproliferation bioactivity was measured using the brine shrimp lethality test. The outcome of the brine shrimp lethality test showed that ethyl acetate and water fraction cytotoxicity were more than 1000 ppm and 337.07 ppm, respectively, at a lethal concentration of 50. Antiproliferative activity analyses were performed using direct hemocytometer counting. The antiproliferation activity data collected were analyzed using one-way ANOVA and Duncan continuous testing. The outcome showed that the water and ethyl acetate RYR antiproliferation activity against MCM B2 cancer cells correlated positively with the increasing concentration of each fraction. Ethyl acetate and water fractions at concentrations of 350 ppm may inhibit the growth of MCM-B2 cancer cells in vitro, reaching 42.63 percent and 39.84 percent, respectively, not significantly different (P < 0.05) with a positive doxorubicin control of 41.24 percent. In conclusion, the ethyl acetate and water fraction of RYR have potent antiproliferation activity against MCM-B2 breast tumor cells.
AKTIVITAS SEDIAAN SALEP EKSTRAK BATANG POHON PISANG AMBON (Musa paradisiaca var sapientum) DALAM PROSES PERSEMBUHAN LUKA PADA MENCIT (Mus musculus albinus) Bayu Febram; Ietje Wientarsih; Bambang Pontjo
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.515 KB) | DOI: 10.22146/tradmedj.8136

Abstract

The objective of the present research is to study the activity of banana stem extract in ointment solutions in the acceleration of wound healing process on mice skins. Totally of 45 mice strain DDY 4-6 weeks old were devided in negativecontrol group (placebo ointment), positive control  group (Betadine® ointment) and Ambon banana stem extract ointment.All mice were aseptically wounded 1-1,5 cm in the anterior region of back skin using a sterile scalpel. The wound wassmeared with the ointment. The pathology anatomy observations was done inday 3, 5, 7, 14 and 21 post wounded. Parameters of the gross lesions (pathology anatomy) observations warecolour of the wound, blood coagulations, scab formations and size of the wound. Parameter for (microscopic lesions) histopathology were infiltrations of inflammatory cells (neutrophils, lymphocytes,macrofages), neo-capillarizations, re-epitelization percentage and the  thickness of fibroblast. All quantitative data were measure using ANOVA and continue with Duncan Test, moreover, the qualitative data were presented descriptively. The result shows that gross lesions observations, the extract ointment group was faster in scab formations and covers the wound without trace compared to the negative control group. The statistical test on the infiltrations of inflammatory cells parameter of the extract ointment group significantly different (P<0.05) compared to the negative control group. Histopatologycal observations shows that Ambon banana stem extract in ointment solutions can increase the infiltrations of inflammatory cells, neo-capillary formations, re-epitelizations percentage andacceleration of fibroblast formations. Base on the result the Ambon banana stems extract in ointment solutions can accelerate the wound healing process and it seems that this solution could be developed and uses for the medical purposes.
Induction of Trichosanthes cucumerina anguina L. var (L. Haines) Hairy Roots Using Agrobacterium rhizogenes ATCC 15834 for Production of Bioactive Protein . CHURIYAH; GUSTAF ADOLF WATTIMENA; BAMBANG PONTJO PRIYOSOERYANTO
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.279 KB) | DOI: 10.5454/mi.5.3.5

Abstract

In the recent years, hairy roots have become a useful model system for the study of bioactive secondary molecule production and protein expression. Hairy roots of Trichosanthes cucumerina var. anguina were obtained by direct inoculation of the plantlet with Agrobacterium rhizogenes ATCC 15834 strain agropine. Six root clones of Trichosanthes cucumerina var. anguina hairy roots TH1, TH2, TH3, TH4, TH6, and TH8 were obtained from the induced hairy roots. The hairy root proteins were extracted with phosphate buffer saline, then fractionated by ammonium sulphate precipitation (80%), dialysis, and gel filtration. The toxicity of the proteins was analyzed using brine shrimp lethality test followed by cytotoxicity test (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay) using HeLa and K-562 cancer cell line. The TH2 root clone showed the highest protein yield (0.83%) and most toxic on BSLT with Lethal Concentration 50 was 5.76 μg mL-1. PCR analysis indicated the integration of rolB gene to the genome of TH2 root clone which was showed by a DNAband of 780 bp on the electrophoretic agarose gel. Protein fractionation of the TH2 root clone resulted in four fractions, one of which the-TH2-3 protein fraction revealed the highest yield (0.29%) and toxicity on BSLT with  LC50 up to 0.92 μg mL-1 . The Cytotoxicity test of the TH2 protein and TH2-3 protein fraction indicated that both of proteins inhibited the proliferation of Hela and K-562 cell withLC up to 49 μg mL-1.
Analysis of osteoblast, osteoclast levels and radiographic patterns in the healing process of bone fractures (preliminary research) Norlaila Sarifah; Lusi Epsilawati; Azhari Azhari; Mieke Hermiawati Satari; Bambang Pontjo Priosoeryanto; Isnur Hatta; Annisa Fitriyana
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 5 No 3 (2021): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v5i3.740

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Objectives: The healing process of a bone fracture goes through many phases. The hard callus phase was critical where the original structure was conducted. The hard callus growth depends on osteoblasts and osteoclasts active, and this condition can be analyzed on the radiograph. This study aimed to examine the analysis of bone fracture healing between osteoblasts and osteoclast numbers and radiographic patterns. Materials and Methods: The study used 12 male Wistar rats with an incomplete fracture in the right femur made by a dental tapered bur with 0.3 mm in length and 0.2 mm in depth. Digital radiographic examinations were carried out on days 0, 5, 10, 17, and 25 after fracturing in a lateral position. Furthermore, a radiographic analysis was performed using Image-J to obtain changes in the value of length and depth in the healing area. The research was conducted to find the radiopaque and radiolucent patterns and the number of osteoblasts and osteoclasts. Results: This study resulted in a change in the radiograph pattern. Callus formation resulted in fracture areas with a smaller distance from day 0 to day 25. The bone healing process begins with granulation tissue formation, followed by the gradual replacement of the connective tissue and bone. This process is comparable to the increase in osteoblasts up to day 25, which blocks bone resorption. Osteoclasts regulate bone resorption, and their number increases after 10 and 17 days to replace bone formation. Osteoclasts decline after 25 days because osteoblasts inhibit them, which control bone formation. Conclusion: The conclusions were obtained there are changes in the radiograph pattern. The radiopaque increased while the radiolucent decreased; the osteoclast pattern tended to be stable and lowered while the osteoblasts increased during the fracture healing process. The correlation of all the factors is very closely related.
Karakteristik Nori dari Campuran Rumput Laut Ulva lactuca dan Eucheuma cottonii Fransiska Rungkat Zakaria; Bambang Pontjo Priosoeryanto; Erniati Erniati; Sajida Sajida
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): Juni 2017
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v12i1.336

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AbstrakRumput laut jenis Ulva lactuca dan Eucheuma cottonii merupakan rumput laut yang dapat dijumpai di perairan Indonesia, akan tetapi pemanfaatannya sebagai produk nori belum dilakukan. Penelitian ini bertujuan untuk memproduksi nori dari rumput laut U. lactuca dan E. cottonii dan menguji karakteristik mutu fisik, kimia dan sensori. Dari hasil penelitian didapatkan bahwa campuran rumput laut U. lactuta dan E. cottonii dapat menghasilkan produk nori yang berkualitas. Hasil uji organoleptik menunjukkan produk nori yang dipanggang lebih dapat diterima dibandingkan nori tidak dipanggang. Karakteristik kimia dan fisik produk berbeda nyata pada taraf 5%. Hasil analisis karakteristik kimia menunjukkan bahwa produk nori rumput laut U. lactuca dan E. cottonii mempunyai kandungan protein yang lebih rendah (18.84%), kandungan karbohidrat yang lebih tinggi (62.31%), kandungan serat pangan yang lebih tinggi (36.76%) dan kapasitas antioksidan yang lebih rendah (43.01%) dibandingkan nori komersial (karbohidrat 41.8%, protein 40%, serat 21.3%, kapasitas antioksidan 51%). Characteristics of the Nori from Mixture of  Ulva  lactuca and Eucheuma cottonii SeaweedsAbstractUlva  lactuca and  Eucheuma  cottonii are seaweed that are available in Indonesia, but their utilization as food product is very limited. This study aimed to produce nori processed from mixture of  U.  lactuca and  E.  cottonii seaweed and evaluate the quality characteristics of the physical, chemical and sensory of the product. The results showed that a mixture of U. lactuca and E.  cottonii seaweed could be processed into the nori. Organoleptic tests showed that roasted nori product were more acceptable than unroasted nori. The chemical and physical characteristics of the product significantly different at the level of 5%. Chemical characteristics showed that the nori product from  U.  lactuca and  E.  cottoniiseaweed contained higher carbohydrate (62.31%), lower protein (18.84%), higher fiber content (36.76%) and slightly lower antioxidant capacity (43.01%) compared to commercial nori (carbohydrate 41.8%, protein 40%, fiber 21.3%, and antioxidant capacity 51%).
Potensi Beras Analog sebagai Alternatif Makanan Pokok untuk Mencegah Penyakit (Degeneratif Potency of Rice Analogue as Staple Food Alternative to Prevent Degenerative Diseases) Slamet Budijanto; Nur Fathonah Sadek; Nancy Dewi Yuliana; Endang Prangdimurt; Bambang Pontjo Priyosoeryanto
JURNAL PANGAN Vol. 25 No. 1 (2016): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v25i1.307

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Perubahan gaya hidup telah menempatkan penyakit degeneratif sebagai penyebab kematian utama, baik di dunia maupun di Indonesia. Salah satu upaya pencegahan penyakit ini dapat dilakukan melalui pengaturan pola makan. Pembuatan beras analog selain bertujuan untuk mendukung program diversifikasi pangan, tetapi juga dapat dirancang dari bahan-bahan tertentu (seperti sorgum, bekatul, dan kedelai) agar memberikan manfaat fungsional khusus bagi kesehatan. Beras analog telah diteliti memiliki aktivitas hipokolesterolemik, nilai indeks glikemik yang rendah, serta menghambat proliferasi sel kanker kolon secara in vitro. Aktivitas tersebut selain berasal dari komponen fitokimia yang terdapat pada bahan-bahan tetapi juga dapat muncul akibat proses pengolahan, seperti terbentuknya pati resisten maupun terlepasnya komponen aktif dari matriks pangan. Hanya saja penambahan komponen fungsional terkadang memberikan dampak yang tidak diinginkan pada atribut sensori. Oleh karena itu, pengembangan beras analog fungsional dari segi sensori harus terus dilakukan supaya dapat diterima dengan baik oleh masyarakat.The changes of lifestyle nowadays bring degenerative diseases as the most leading cause of death, both in worldwide and Indonesia. It has been very well known that incident of the diseases could be prevented through food diet. Not only aimed to support the government’s diversification program, rice analogue development could be designed from certain materials (such as sorghum, rice bran, and soybean) to provide specific functional health benefit. Some reports showed that rice analogue has low glycemic index, as well as its activity in lowering cholesterol level and inhibiting the proliferation of in vitro colon cancer cell. The activity was expected to come from the phytochemical compounds naturally present in the ingredients and might also arise during the processing, such as the formation of resistant starches as well as the release of the active compounds from food matrix. However, the addition of functional ingredients sometime impacted on undesirable sensory attributes. Therefore, all sensory attributes of functional rice analogue should be improved to achieve the increment of public acceptance.
Aktivitas Minyak Ikan dalam Menghambat Preneoplasia Kolon Mencit yang Diinduksi Azoksimetan dan Dextran Sodium Sulfate KUSMARDI KUSMARDI; BAMBANG PONTJO PRIOSOERYANTO; EVA HARLINA; SANTOSO CORNAIN
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Epidemiologic studies of dietary marine n-3 fatty acids and risk of colorectal cancer have been inconsistent. The study was conducted to understand the inhibitory effect of fish oil in mice with colorectal preneoplasia induced by azoxymethane (AOM) and dextran sodium sulfate (DSS). In this study, Balb/c mice was induced by AOM 10 mg/kg body weight followed by administration of 1 % DSS during a week. Fish oil administrated orally at a dose of 1.5, 3, and 6 mg per day. Histopathological examination of the colon tissue (hematoxylin-eosin staining) was done by counting the number of inflammation, and hyperplasia foci, the number of mitosis epithelial cells, and the scores of dysplasia in ten visual fields. In the second month, decreasing in the number of inflammation foci occurred between the control and low dose groups with medium and high dose groups (p<0,05). While in the third and fourth month, decreasing in the number of inflammation foci were observed in all treatment group (p<0.001). The number of mitotic colonic crypt epithelial cells was statistically significant between control group and treatment groups, initially observed since the second month. In the second month to the fourth month, generally increasing of doses of fish oil has decreased the number of hyperplasia foci. Our research indicates that fish oil also inhibits the occurrence of dysplasia. In the third and fourth month, dysplasia was found in the control group. Dysplasia was found only in the fouth month in the treatment group.
Evaluasi Karakter Agro-Morfologi pada 20 Genotipe Hasil Seleksi Temu Hitam (Curcuma aeruginosa Roxb.): Evaluasi Karakter Agro-Morfologi pada 20 Genotipe Hasil Seleksi Temu Hitam (Curcuma aeruginosa Roxb.) Waras Nurcholis; Hartanti Hartanti; Suryani Suryani; Bambang Pontjo Priosoeryanto
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 2 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.273 KB) | DOI: 10.33019/agrosainstek.v3i2.58

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The present study aimed to evaluate different agro-morphological traits among twenty genotypes of Curcuma aeruginosa Roxb. and three varieties of Curcuma zanthorrhiza Roxb. Agro-morphological data traits were investigated based on qualitative and quantitative parameters from PPVFRA descriptors with modification. All the recorded data was analyzed through SPSS 16.0 and R 3.4.2 for ANOVA and similarity analysis, respectively. Significant differences (P <0.05) were observed in the traits studied of the habit of the rhizome, the shape of the rhizome, length of primary rhizome, number of mother rhizome, plant height, pseudostem diameter, number of leaves, leaf length, leaf width, and number of shoots. Hierarchical cluster analysis (HCA) classified the genotypes into three groups. The principal component analysis (PCA) were showed consistent with results of the HCA. These genotypes could be used as novel genotype in agricultural biochemistry for breeding programs.
Co-Authors . CHURIYAH . Darmawi Adhi Rahmat Hariyadi Aditya Primawidyawan Agus Susanto Ahmad Aulia Ahmad Aulia Andra Hendriarto Andri Hardianto Andri Lubis Andri Prasetiyo Anggraeny Putri Sekar Palupi Anggraini, Lutvi Anissa Canintika Anita Esfandiari Annisa Fitriyana Ardi Sandriya Ari Fahrial Syam Ari Fahrial Syam Aria Kekalih Asri, Lia Amelia Tresna Wulan Aulia Andi Mustika Auliya Ilmiawati Az Zahra, Shafiyyah Azhari Azhari Bambang Sunendar Purwasasmita, Bambang Sunendar Bayu Febram Bayu Febram Bayu Febram Prasetyo Bethy Suryawathy Hernowo Betty S.L. J Betty Sri Laksmi Jenie Budi Arifin Chudahman Manan Chudahman Manan Dahlia Sutanto Daldiyono Daldiyono Daldiyono Daldiyono Darmawi D Deddy Muchtadi Dede Robiatul Adawiyah Deni Noviana Denny Widaya Lukman Dewi Fatma Suniarti Dewi Ratih Agungpriyono Djarot Sasongko Hami Seno Dwi Kesuma Sari Dyah Sulistyani Rahayu Edy Djauhari Purwakusumah Eka Nurul Qomaliyah Endang Prangdimurt Endang Prangdimurti Endang Sjamsudin Erniati, Erniati Esti Mumpuni Eva Harlina Evy Damayanthi Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fachrul Razi Fadila Raisyadikara Failasufa, Adzkia Farra Sasmita Franky Oscar, Franky Fransiska R. Zakaria Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria Gatot T. Mulyadi Gatot Trimulyadi Rekso Gunanati Soedjono Gunanti Gunanti . GUSTAF ADOLF WATTIMENA Hamny Sofyan Hartanti Hartanti Hartanti Hartanti, Hartanti Hasim Hasim Hatibie, Mendy Juniaty Hatta, Isnur Herwin Pisestyani Hidayat Syarief Husnawati, . I Made Kardena I Nyoman Suarsana I Nyoman Suartha I Wayan Gorda I wayan Teguh Wibawan IETJE WIENTARSIH Irmanida Batubara Iwan Harjono Utama Jimmy Pangihutan Juliana, Dara Kazuyuki Uchida Koeswinarning Sigit Komariah Komariah Kreshanti, Prasetyanugraheni Kuroki, Yutaka KUSMARDI KUSMARDI Kusumawati, Nina Tri Lanniari, Nafrina Latief, Benny Syariefsjah Latifah Darusman Lilis Nuraida Lina Noviyanti Sutardi Lisni Noraida Lubis, Septina Sabilla Lucky Riawan Lusi Epsilawati Madyastuti, Rini Mahdi Abrar Makmuri Yusuf MARIA BINTANG Maya Shofa Mieke Hemiawati Satari Mieke Hemiawati Satari Mieke Hermiawati Satari Miksusanti Miksusanti Mirnawati Soedarwanto Mirnawati Soedarwanto Mirnawati Soedarwanto Monica Septiani Muharty, Annisya Najmah Najmah Nancy Dewi Yuliana Naoaki Misawa NASRIANTI SYAM, NASRIANTI Nina Kusumawati Nur Fathonah Sadek Nurhidayat - Nurhidayat, Nurhidayat Okabayashi, Tamaki Prangdimurt, Endang Priskila Priskila Putu Angga Wiradana Putu Eka Sudaryatma Rachmi Ridho Rahmadansah Rahmadansah Rahminiwati, Min Rahyussalim Ratna Puspita Rayendra, Raendi Retno Wulansari Rifki Septawendar Riki Siswandi Rimbawan , Rini, Dian Cipta Ripta Mustafa Nugraha Risa Tiuria Riski Rostantinata Riski Rostantinata Rizal Syarief Rizal Syarief Rizki Fitria Rizki Rostantinata Ros Sumarny Rostantinata, Riski Ryoji Yamaguchi Sabri Mustafa Sadek, Nur Fathonah Safrida Safrida Sajida Sajida Samuel Leonardo Silitonga SANTOSO CORNAIN Sarifah, Norlaila Sebayang, Shalina SILMI MARIYA Slamet Budijanto Sri Anna Marliyati Sri Estuningsih Sri Kayati Widyastuti Subangkit, Mawar Sugeng Supriadi, Sugeng Sukasah, Chaula Luthfia Sunardhi Mangundjaja, Sunardhi Surachmi Setiyaningsih Surachmi Setyaninigsih Surya Pangidoan Nasution Suryani Suryani Suryani Suryani Susi Susanah Susumu Tateyama Syamsudin Abdillah Takeshi Katayama Tangkonda, Elisabet Tena Djuartina, Tena Toshisada Suzuki Tri Isyana Tungga Dewi Tri Isyani Tungga Dewi Tungga Dewi, Tri Isyani Tutik Wresdiyati Ummu Balqis Vetnizah Juniantito Wahyuni, Sri Waras Nurcholis Wasmen Manalu Wati, Vivi Septya Weko Adhiarto, Weko Wiranti, Rahayu Woro Yonathan Arderian Mantik Ziad Alaztha