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Sunarto Sunarto
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Journal : Paediatrica Indonesiana

 1 
Translocation ETS leukemia-acute myeloid leukemia 1 (TEL-AML1) gene fusion in childhood acute lymphoblastic leukemia Sri Mulatsih; Yeow Liang; Allen Yeoh; Sutaryo Sutaryo; Sunarto Sunarto
Paediatrica Indonesiana Vol 49 No 5 (2009): September 2009
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.364 KB) | DOI: 10.14238/pi49.5.2009.270-5

Abstract

Background Acute lymphoblastic leukemia (ALL) in childrenis a heterogeneous disease with different subtypes based on their cellular and molecular characteristics. This condition wouldinfluence the treatment outcome and subsequent risk for relapse. Accurate assignment of individual patients to risk groups is a critical issue for better outcome. TEL-AML1 gene fusion is themost frequent in childhood ALL.Objective The aim of this study was to investigate the incidenceofTEL-AML1 children with ALL in Sardjito Hospital.Methods This was a cross sectional study. In this preliminarystudy, we used nested reverse-transcriptase polymerase chainreaction (RT-PCR) to analyze the present of TEL-AML1 genefusion in bone marrow sample of childhood ALL patients.Results We analyzed 41 samples. Out of these, 30 (73%) wereamplified. Twenry three out of 30 ALL patients with good medicalrecord were analyzed for this gene fusion. Out of 30 patients, there were five patients (17%) with TEL-AML1-positive gene fusion and 25 (83%) were TEL-AML1-negative. Among five patients with TEL-AML1-positive gene fusion, four patients (80%) were one year to less than 10 year old. All of the patients (100%) were with leukocyte < 50x109/L.Conclusions TEL-AML1 gene fusion was found in 17 % ofsamples. This gene fusion was more frequent in standard risk group (based on age and leukocyte). These data must be clarified with more samples. RT-PCR must be apply in all center as one part of improving diagnostic quality, especially in managing leukemia patients.
Variable Severity of B-Thalassemia/Hemoglobin E Disease - the Genetic Factors Sunarto Sunarto
Paediatrica Indonesiana Vol 37 No 1-2 (1997): January - February 1997
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi37.1-2.1997.6-12

Abstract

Hemoglobin E (Hb E) is prevalent in Southeast Asia. Heterozygotes give no manifestation, even homozygotes show no manifestation or only slight anemia. However, compound heterozygote with 6-thalassemia gives anemia with variable severity. The severely affected individuals show anemia similar to homozygous 6-thalassemia. Many factors play a role in determining the severity. SThnl/6E patients have increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. The increased GSH-Px is thought to be needed for the elimination of hydrogen peroxide produced by SOD decomposition of peroxide. The content of antioxidants vitamine E and C is reduced, whereas MDA, the final product of lipid peroxidation increases significantly. The genotype of the Xmn 1 polymorphism, -158 bases upstream from the transcription site of the n-globin and the level of Hb F are associated with clinical severity, but the extent of the S' -globin mRNA cryptic splicing is more associated with the severity of tire manifestation than does the pattern of the Xmn I polymorphism.
Co-Authors A A Musyaffa A.A Musyaffa Abdul Rachman Abdul Rozak Ali Maftuhin Achmad Asrori Addi Mardi Harnanto Adelina Adelina Adi Martono Adriyani Adam Afrilia Miratul Ulfa Aga Ardhianto Feryyanshah Aga Dwi Sintha Agung Budi Raharjo Agus Naryoso Agus Rahmad Hidayat Agustianto, Wahyu Ainul Maftuchah Alamsyah Alamsyah Aldhila Gusta H. Yoga Alhadi Alhadi Allen Yeoh Alya Dinia Asyfiqi Masykur Alya Khoirun Nisa Andre Lukita Andrean Widiyantoro Angga Dwi Pratama Angga Widhi Saputro Anindya Ratna Pratiwi Anisatul Luthfia Annisa Arum Putri Anwar Anwar Ari Sugeng Arimby Palera Armeina Nur Rachmawati Asikin, Hijrah Asmawati Asmawati Asri Nugraheningtyas Ayi Yustiati Bagus Sembodo Bagus Susetyo Bambang Subali Bambang Sudarmanto Bambang Wasito Adi Baskoro Adi Prayitno Bilqisti Bilqisti Binti Khoirur Rofiah Chairunnisa Chairunnisa Chandra Wijaya Charles Parningotan Haratua Simanjuntak Dandi Wahyu Agung Darusman Darusman David David Dedy Sugianto Dendy Kurniawan Devi Ayu Kusuma Putri Dewi Hasanah Dilla Mardianty Dona Kristiawan Dowes Ardi Nugroho Dwi Safrudin Dwi Septiani Dwiky Erlangga Edhi Martono Eka Prasetyanta Endang D Endang Djuana Erlinda Erlinda Erni Murniarti, Erni Ervin Devi Pratiwi Esti Dyah Utami Evi Gravitiani Fahad Nuraini Farah Azizzah Faridah Laeli Syarifah Fatma Lestari Ferdiansyah Ferdiansyah Feri Setyowibowo Ferrianto G Ferrianto Gozali Fige Arief Kusumo Fitri Sagantha Florenchia Yohana Tellu Fredi Ganda Putra Fuad Abdul Fattah Furi styo Siskawati Hanif Nasiatul Baroroh Hari Wahyudi Harini Harini Harliyandri Iqbal Nugroho Harry Supriyono Heti Herawati I Made Sukanadi Igviro Yurki Lailina Iin Triyanti Ilham Futaki Ilyas Ilyas Imam Sufardi Inas Khairani Indra Suharman Intan Sonia Apriani Irfanda Rizki Harmono Sejati Iskandar Iskandar Iskandar Suhardi Isna Putri Indayani Iva Yuniasih Jennifer Jennifer Kandar Kandar Katisya Abrina Prastyanti Ketut Ima Ismara Khaeriya Megauleng Kidung Sukma Asmarani Kristiani Kristiani Kuat Rahardjo Kuat Rahardjo T.S Kuat Rahardjo TS Kurnia Roossenda Langgeng Wahyu Santosa Latifatul Azizah Leidena Sekar Negari Leny Noviani Lia Faiqoh Luthfi Anis Muadzin M. Mudrik H. Rohman M. Ngainul Malawani M. Rudyansyah Ismail Marina Silalahi Mariska Diah Kusumasari Mega Laksmini Syamsudin Mega Nugraha Mintasih Indriayu Moch Adhitya Rifka Wibowo Mochamad Candra Wirawan Arief Muchamad Yulianto Muhammad Abduh Muhammad Andi Auliya Hakim MUHAMMAD INDRAWAN Muhammad Rizki Romadhon Muhammad Sabandi Mustamin Nafik Salafiyah Nanang Supriadi Naris Dyah Prasetyawati Neazar Astina Prabawani Niken Ambar Pratiwi Niko Sangaji Noor Sudiyati Nugroho Wahyu Sumartono Nur Amalia Choirani Nur Amalia Choironi Nur Dzaedzatul H Nur Faizin Nursetya Danusaputra Nurul Faizatus Sholikah Nuryanti Nuryanti Pandansari Kusumo Ponendi Hidayat Putri Oktaviana Rahmi Lubis Rani Rakhmaputri Wiranto Rapiko Rapiko Ratnapuri, Adisti Regina Tutik Padmaningrum Retno Dewi Kurniasari Ridhah Taqwa Rihadini Rihadini Rizkan Faif Fasani Robert Adriaan Philipus Rosalia H S Rosalia H Subrata S. Jazilah Sahora Dina Pangesty Salman Alfarisy Totalia Sandy Kristiara Sari Rosiati Nur Khasanah Sigid Sudaryanto Sisca Indriyani Sischa Wahyu Dwiningsih Siti Alliyah Siti Asiah Siti Kartika Sari Siti Marwati Siti Ubaidah Sitinjak, Rivaldi Slamet Haryono Slamet Mardiyanto Rahayu Sri Budi Lestari Sri Mulyani Sri Widowati Herieningsih Sri Woro Asih Sudarno Sudarno Sulton Sulton Sumardino Sumardino Suparmi Suparmi Supriyanto Supriyanto Suriani Rauf Sutaryo Sutaryo Syahrun Syahrun Syahrun, Syahrun Syahrun Syaiko Rosyidi Tandiyo Pradekso Testian Yushli Ana Tiara Handayani Titi Yuli Astuti Titik Kuntari Titin Nurfiatin Toto Tohir Totok Trisaputro Tri Melisa Selviani Trisnawiyana Trisnawiyana Triyono Lukmantoro Tuti Sri Suhesti Tutik Fitri Wijayanti Ubad Badrudin Umil Muhsinin Utami Utami Vania Reani Valmai Wahyu Lestari Wahyu Yudha Pranoto Wahyu Yun Santoso Wahyuningtyas, Riska Septia Widha Sunarno Wijayanti Puji Lestari Winda Saputri Wiryanto Wiryanto Wiwid Noor Rakhmad Yasarah Diswari Ditiya Yeow Liang Yoseph Santosa Yudi Prana Hikmat Yuli Andriani Yuliyati Yuliyati Yun Ismi Wulandari Yuyuk Liana Zahidah Hasan Zaky Mubarok, Zaky Zulfikar Zulfikar Zulfikri Suleman