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All Journal Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Jurnal Kesehatan Andalas Jurnal Riset Kimia Jurnal Kreativitas PKM Acta Biochimica Indonesiana Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Majalah Kedokteran Andalas Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Ilmu Kesehatan Indonesia (JIKSI) Indonesian Journal of Cancer Jurnal Riset Ilmiah Jurnal Sains, Teknologi dan Kesehatan Bioma : Jurnal Ilmiah Biologi
Elmatris Sy
Department Of Biochemistry Faculty Of Medicine Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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ISOLATION OF AnsB GENE FRAGMENT ENCODING L-ASPARAGINASE 2 ENZYME FROM Serratia plymuthica UBCF_13 AND IT’S IN-SILICO DOMAIN CHARACTERISTIC Ananda, Abi Awfa Rahman; Nursyafi, Fauzan Syarif; Aliya, Lisana Shidiqqin; Hasibuan, Imron Martua; Alioes, Yustini; Endrinaldi , Endrinaldi; Adrial, Adrial; Elmatris , Elmatris; Jamsari, Jamsari; Lily Syukriani
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/n5mfhy69

Abstract

Acute lymphoblastic leukemia(ALL) is one of the cancer diseases often occurs in children and causes high mortality in children. One of the chemotherapy treatment suggested is using L-Asparaginase 2. However due to its difficult production process making this approach expensive for the public. Therefore production technology of this enzyme is crucial enabling cheaper for ALL treatment. This study aimed to isolate the AnsB gene sequence from Serratia plymuthica UBCF_13 and perform its further in-silico analysis. The research was started by designing specific primers for the AnsB gene, isolating the AnsB gene fragment using PCR-based approach, sequencing the AnsB gene fragment, cloning the fragment to the plasmid vector and further transformed into E. coli DH5α cell. Further data analysis was carried out using some bioinformatics tools such as BLAST, MEGA X, I-TASSER InterPro. Sequence data result successfully verified that the full length of AnsB gene is 1047 bp. InterPro analysis indicated that the L-Asparaginase 2 from S. plymuthica UBCF_13 has 2 domains, namely L-Asparaginase N-terminal spanning from amino acid 26 to 216, while its C-terminal spanned from amino acid 235 to 345. The physical fragment of the gene was also successfully cloned to the pGEM-T Easy vector and subsequently transformed into E. coli DH5α cell. This result provided information for alternative sources of L-Asparaginase 2 and it’s possible engineering.
SYNERGISTIC COMBINATION OF JAVA PLUM (SYZYGIUMCUMINI(L.) SKEELS) LEAF EXTRACT AND VITAMIN C ON ASPARTATE AMINOTRANSFERASE AND ALANINE AMINOTRANSFERASE LEVELS IN LEAD ACETATE-INDUCED RAT Ikhsan, M; Rita, Rauza Sukma; Elmatris, Elmatris; Alioes, Yustini; Mahata, Liganda Endo; Almurdi, Almurdi
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 3 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, September 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i3.1708

Abstract

Lead (Pb) is a toxic metal that is used to trigger oxidative stress. Oxidative stress causes damage to organs, especially the liver increasing aspartate aminotransferase (AST) and alanine aminotransferase (ALT). This study aims to determine the synergistic effect of antioxidant as Java plum leaf extract and vitamin C together on lead-induced levels of AST and ALT in rats.This type of research is true experimental research with a randomized posttest-only-control group design. The study used 20 male white rats which were divided into 4 groups. The treatment was carried out for 30 days. The examination for AST and ALT levels using the Micro Lab 300 with the Sample Star method. Data analysis was performed by using One-way Anova and Post Hoc-Turkey HSD.In this study, The results were obtained in the form of a decrease in AST and ALT levels in rats that had been induced lead acetate 40 mg/KgBW by administration of Java plum leaf extract dose 75 mg/KgBW and vitamin C dose 35 mg/KgBW as well as the administration of Java plum leaf extract at a dose of 150 mg / KgBB and vitamin C dose of 75 mg / KgBB with significant influence at the lower dose (p=0,01).It can be concluded that administrating of Java plum leaf extract dose 75 mg/KgBB and vitamin C dose 35 mg/KgBB was effective to protect the liver based on the decrease of AST and ALT levels in rats that had been induced lead acetate dose 40 mg/KgBB
KORELASI KONSUMSI MAKRONUTRIEN DENGAN PROFIL LIPID PADA PETUGAS KESEHATAN YANG OBESITAS DI RSUP M. DJAMIL PADANG TAHUN 2022 Juana, Aulia Luthfi Putri; Sy, Elmatris; Lipoeto, Nur Indrawati
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 3 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, September 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i3.1796

Abstract

Dyslipidemia is a health problem that needs attention because the incidence of dyslipidemia in Indonesia continues to increase from year to year. Macronutrient intake in a diet can influence a person's lipid profile. This study aims to determine the correlation between macronutrient consumption in obese health workers at RSUP Dr. M. Djamil Padang in 2022.  This research is an analytical research with a cross sectional study design on secondary data from diet development research on health workers at RSUP Dr. M. Djamil Padang who is obese in 2022 with research subjects of 40 respondents taken using total sampling technique. Data analysis used univariate and bivariate analysis with the Pearson Spearman correlation test. Correlation is declared significant if p<0,05.  The research results showed that the highest mean macronutrient consumption was carbohydrates (219.92 ± 74.21), then fat (64.30 ± 28.35) and the lowest was protein (62.56 ± 18.74). Respondents also had lipid profile values that were in the normal range for total cholesterol (157.75 ± 51.58) and triglycerides (125.33 ± 33.21) and there was an increase in LDL (106.46 ± 40.13) and HDL ( 76.35 ± 27.54).  From this study it was concluded that there was a significant correlation between carbohydrate consumption and total cholesterol (p value = 0.026; r = 0.351) and LDL (p value = 0.008; r = 0.415), but there was no correlation between carbohydrates, protein and fat with the profile other lipids in obese health workers at RSUP Dr. M. Djamil Padang.
Hubungan Karakteristik Ibu dan Dukungan Suami dengan Penggunaan MKJP di Puskesmas Lubuk Kilangan Efandra, Rizky; Masrul, Masrul; Aprilia, Dinda; Aladin, Aladin; Elmatris, Elmatris; Silvia, Nelmi
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 3 (2025): September 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i3.1367

Abstract

Latar Belakang: Metode kontrasepsi jangka panjang (MKJP) merupakan metode untuk mencegah kehamilan lebih dari tiga tahun. Prevalensi MKJP nasional tahun 2022 sebesar 22,41 % dari target RPJMN 28,39% pada tahun 2024. Penggunaan kontrasepsi masih didominasi oleh non-MKJP.  Penggunaan MKJP di Kecamatan Lubuk Kilangan tahun 2022 sebesar 15,3%. Objektif: Penelitian ini bertujuan untuk mengetahui hubungan karakteristik ibu dan dukungan suami dengan penggunaan MKJP pada akseptor KB di Puskesmas Lubuk Kilangan. Metode: Penelitian ini merupakan penelitian analitik dengan desain cross sectional. Penelitian ini dilakukan di Puskesmas Lubuk Kilangan dengan jumlah sampel 200 akseptor KB. Pengambilan sampel dengan consecutive sampling menggunakan kuesioner. Analisis data dilakukan menggunakan uji chi square dan multivariat regresi logistik. Hasil: Hasil penelitian menunjukkan lebih dari separuh ibu dengan usia tidak berisiko (51,0%) dan paritas tinggi (55,5%); sebagian besar ibu memiliki tingkat pendidikan tinggi (71,0%), pengetahuan baik (80,0%), dan mendapatkan dukungan suami (73,0%). Kesimpulan: Terdapat hubungan antara karakteristik ibu (usia, paritas, dan pengetahuan) dan dukungan suami dengan penggunaan MKJP di Puskesmas Lubuk Kilangan. Faktor paling dominan mempengaruhi penggunaan MKJP adalah dukungan suami.
Hubungan Status Imunisasi dengan Kejadian Stunting pada Balita di Wilayah Kerja Puskesmas Andalas Kota Padang Riona, Vira Harisa; Masnadi, Nice Rachmawati; Firdawati, Firdawati; Harmen, Anggia Perdana; Elmatris, Elmatris; Fadila, Zurayya
Jurnal Kesehatan Andalas Vol. 14 No. 1 (2025): March 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Stunting is a linear growth failure caused by chronic malnutrition and recurrent infections that mainly occur in the first 1000 days of life. Infectious diseases can be prevented by providing immunizations. Immunization is one way to increase one's immunity against disease exposure actively. Objective: To determined the relation of immunization status with the incidence of stunting in children. Methods: Analytical research with a case-control design on 98 under-five children in the working area of Andalas Health Center was conducted from June to November 2024. Sampling was done by simple random sampling. Anthropometry was measured using body length or height measurements according to standards and data were collected by questionnaire. The data analysis used was Chi-square. Results: The incidence of stunting in the working area of Andalas Health Center was mainly (89.8%) experienced by children aged 24 - 59 months and more than half (59.2%) were female and most (81.6%) had an incomplete basic immunization status. The study also found that more than half (59.2%) of mothers' education in stunted children was high school and most (81.6%) occurred in mothers who did not work. There is a statistically significant relation between immunization status and the incidence of stunting in children, with p = 0.027 (p value <0.05) and OR value = 3.065. Conclusion: This means that under-five children who do not have complete basic immunization status have a 3.065 times higher chance of stunting than under-five children with complete basic immunization status. Keywords:  immunization status, under-five children, stunting
Hubungan tingkat aktivitas fisik dengan status gizi mahasiswa Fakultas Kedokteran Unand pada pandemi COVID-19 Nurullah, Afi Putra Perdana; Desmawati, Desmawati; Abdiana, Abdiana; Rita, Rauza Sukma; Khaira, Fathiyyatul; Elmatris, Elmatris
Majalah Kedokteran Andalas Vol. 48 No. 3 (2025): MKA July 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v48.i3.p266-277.2025

Abstract

Objective: To determine the relationship between levels of physical activity and the nutritional status of the year 2019 undergraduate medical students at Andalas University during the COVID-19 pandemic; Method: This study was a quantitative study using a cross-sectional method with 151 respondents held in September 2022 at Medical Faculty Andalas University. The study used an IPAQ-short form questionnaire to ensure the levels of physical activities and direct anthropometry to measure the nutritional status. Analysis between the physical activity data and the nutritional status data was tested using the Pearson chi-square test; Result: The result of the study showed that 42,4% of respondents have low physical activity, 44,4% of respondents have normal nutritional status, the chi-square test between physical activity and nutritional status of was p=0,349; Conclusion: there was no significant relationship between levels of physical activity and the nutritional status of undergraduate medical students at Andalas University during the COVID-19 pandemic. It is suggested that improving physical activity amongst undergraduate students is highly encouraged as it prevents non-communicable diseases.
Efek Pemberian Mesenchymal Stem Cell Wharton's Jelly (MSC-WJ) terhadap Gambaran Histologi Pankreas Hewan Model Diabetes Melitus Tipe 1 Endrinaldi, Endrinaldi; Tofrizal, Tofrizal; Elmatris, Elmatris; Yarni, Sisca Dwi
Majalah Kedokteran Andalas Vol. 47 No. 2 (2024): MKA April 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i2.p148-155.2024

Abstract

Objective: This study was conducted to determine the effect of administering Mesenchymal Stem Cell Wharton Jelly (MSC-WJ) on the histological appearance of the pancreas of rat in a type 1 diabetes mellitus model. Methods: This research is an experimental research with a Posttest-Only Control Group Design. The sample consisted of 3 groups (Normal, T1DM, T1DM + MSC-WJ). The treatment group was treated with MSC-WJ for 4 weeks. Data analysis used One Way ANOVA and Post Hoc Bonferroni tests. Results: WJ-MSC can increase the mean diameter and area of the islets of Langerhans in the T1DM rat group from 98.08 mm – 126.85 mm and from 4911.95 mm2 – 8995.35 mm2, but this difference is not yet significant (p > 0.05). Conclusion This research shows that there is improvement in pancreatic damage by administering WJ-MSC to diabetic rat due to alloxan induction.
HUBUNGAN PENGGUNAAN ALAT KONTRASEPSI HORMONAL DENGAN STABILITAS TEAR FILM : SEBUAH TINJAUAN NARATIF Praja, Fathira Luthfi Nuresa; Hendriati, Hendriati; Elmatris, Elmatris; Vitresia, Havriza; Irawati, Lili; Mahata, Liganda Endo
SINERGI : Jurnal Riset Ilmiah Vol. 2 No. 6 (2025): SINERGI : Jurnal Riset Ilmiah, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/sinergi.v2i6.1329

Abstract

Background : Tear film is a fluid-filled layer that protects the eyes from the external environment. This layer consists of three layers, namely lipid, aqueous and mucin. Tear film stability is seen as one of the main characteristics of eye health. failure to maintain balance Tear film can cause dry eyes. One of the causes of dry eyes is an imbalance of steroid sex hormones such as estrogen, progesterone and androgens. These hormones have mRNA receptors on the surface tissues of the eye that are susceptible to hormonal contraceptives. Objective : This literature study aims to explore various original research literature on the relationship between the use of hormonal contraceptives and the stability of Tear film.  Methods : Narrative reviews of literature searched through the Pubmed and Google Scholar databases, then selected based on established inclusion and exclusion criteria. Results : There are 6 literatures with 444 women of childbearing age with an age range of 20-45 years who use the TBUT and Schirmer examinations. Conclusion : There is no relationship between the use of hormonal contraceptives and the stability of tear film
EVALUASI PENEGAKAN DIAGNOSIS TUBERKULOSIS ANAK BERDASARKAN PETUNJUK TEKNIS TUBERKULOSIS ANAK 2016 DI RSU BMC PADANG Resti Ayu Safitri; Finny Fitry Yani; Al Hafiz; Rinang Mariko; Rima Semiarty; Elmatris Elmatris
SINERGI : Jurnal Riset Ilmiah Vol. 3 No. 6 (2026): SINERGI : Jurnal Riset Ilmiah, Juni 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/sinergi.v3i6.2639

Abstract

Background: Tuberculosis (TB) is a contagious infectious disease that is still the main cause of health problems and one of the main causes of death in children in the world. The main problem in diagnosing TB in children is that it has not been properly socialized, understood, and implemented by most general practitioners and pediatric specialists, according to the 2016 Technical Guidelines for the Management and Management of Childhood Tuberculosis. Objective: The diagnosis of TB is mostly based on clinical considerations without making efforts to search for bacteriological confirmation, so evaluation is needed towards the implementation of the diagnosis of TB in children based on the 2016 Technical Guidelines for the Management and Management of Childhood Tuberculosis algorithm at RSU BMC Padang. Methods: This type of research is a descriptive study with a cross-sectional retrospective study design using medical record data from pediatric TB patients recorded at the children's clinic at RSU BMC Padang in 2022. The research was conducted at RSU BMC Padang for six months. The sampling technique used was total sampling with a sample size of 137 data. Results: Most patients had symptoms of chronic cough (76.6%), more than half had fever for ≥ 2 weeks (62.8%), weight loss (67.2%), and general malaise (90.5%). Only a small portion (8%) of pediatric TB patients underwent TCM examination, and the majority (89.8%) underwent chest x-rays, and generally (97.1%) underwent tuberculin. All patients were diagnosed with clinical pediatric TB. Conclusion: From the research that has been carried out, it can be concluded that almost all children's TB is diagnosed positively, without implementing the complete diagnosis algorithm in the 2016 Technical Guidelines for the Management and Management of Childhood Tuberculosis. Completeness of supporting examinations, especially TCM, must be paid attention to by the clinician so that all algorithms are fulfilled.
IDENTIFIKASI MASALAH DAN PENYULUHAN KESEHATAN PADA LANJUT USIA DI NAGARI SUMANIAK Aisyah Elliyanti; Dian Pertiwi; Arina Widya Murni; Zelly Dia Rofinda; Yulistini Yulistini; Afriwardi Afriwardi; Eti Yerizel; Siti Nurhajjah; Elmatris Sy; Elizabeth Bahar
Jurnal Hilirisasi IPTEKS Vol. 2 No. 1 (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v2i1.354

Abstract

Pelayanan kesehatan terhadap lanjut usia (lansia) perlu mendapatkan perhatian khusus, karena lansia rentan terhadap penyakit Tujuan: Pengabdian masyarakat ini dilakukan dengan tujuan untuk mengidentifikasi faktor risiko penyakit dan memberikan penyuluhan pada kelompok lanjut usia di Kenagarian Sumanik. Metode: Kegiatan yang dilakukan adalah pemeriksaan kesehatan lanjut usia dan pengkajian psikologis dengan menggunakan kuisioner DASS 42. Selanjutnya dilakukan penyuluhan kesehatan dengan harapan meningkatkan pengetahuan para lansia sehingga mampu mengidentifikasi tanda bahaya dan mendapat pertolongan lebih awal. Hasil: Kegiatan dihadiri oleh 63 orang warga, 31 orang diantaranya di atas usia 60 tahun. Hasil pemeriksaan ditemukan 22 orang mengalami hipertensi stadium 2, dan 5 orang menderita diabetes yang sebelumnya sudah diketahui akan tetapi kontrol tidak teratur. Pada pemeriksaan mata terdapat 25 orang menderita gangguan refraksi, 5 orang ekstensi lensa dan dirujuk ke RSUD melalui Puskesmas. Dari kajian psikologis didapatkan 5 orang memiliki gejala depresi, 15 orang dengan ansietas dan 48 orang mengalami stress-psikologis. Kesimpulan: Penyuluhan kesehatan sangat diperlukan terutama kelompok lanjut usia supaya lansia dapat mengenal tanda-tanda penyakit sehingga dapat pelayanan kesehatan lebih dini. Aktivitas fisik seperti berolahraga diperlukan namun lansia perlu mengetahui jenis olah raga yang tepat sesuai dengan usia.
Co-Authors Abdiana Abdiana, Abdiana Adrial Adrial, Adrial Afi Putra Perdana Nurullah Afifah Aqilatul Faridah Putri Wirza Afriani, Nita Afriwardi Afriwardi Aisyah Elliyanti Al Hafiz ALADIN ALADIN Alhakim, Hafidh Taufiq Aliya, Lisana Shidiqqin Almurdi Almurdi Ananda, Abi Awfa Rahman Anavelda, Aura Putri Andani Eka Putra Angga Putra Perdana Annisa Rusdi Antonius, Puja Agung Aprilia, Dinda Ariadi Ariadi Arifuddin Arifuddin Arina Widya Murni Ashal, Taufik Asterina Asterina Astiena, Adila Kasni Aswiyanti Asri Aswiyanti Asri Aulia Ulfa Azharudina, Adiba Azura Darmawan Beby Rahim Beni Indra, Beni Bobby Indra Utama Dara Afifah Azmi Deddy Saputra Defrin, Defrin Delmi Sulastri Desmawati Desmawati Dessy Arisanti Dessy Arisanti Desy Nofita Sari Dia Rofinda, Zelly Dia Dian Pertiwi Dian Pertiwi Dwi Yulia Efandra, Rizky Efrida Efrida Eka Nofita Elisda Yusra Elizabeth Bahar Elizabeth Bahar Elly Usman Endrinaldi Endrinaldi , Endrinaldi Erly Erly Esy Afrianti Eti Yerizel Etriyel Etriyel Fachzi Fitri Fathiyyatul Khaira Fathiyyatul Khaira, Fathiyyatul Feni Andriani Finny Fitry Yani Firdawati Firdawati Fitria, Henni Fortuna, Fory Hanum, Fathiya Juwita Harmen, Anggia Perdana Hasibuan, Imron Martua Hasmiwati Hendriati, Hendriati Husni Husni Husnil Kadri Ida Rahmah Burhan Ida Rahmah Burhan Iffah, Uliy Ilahi, Fitratul Ilmiawati, Ilmiawati Ivan Maulana Fakh Jamsari Jamsari Juana, Aulia Luthfi Putri Julizar Julizar Julizar Nazar Kemala Sayuti Laila Isrona Lili Irawati Lili Irawati Lily Syukriani M Ikhsan M. Setia Budi Mahata, Liganda Endo Masnadi, Nice Rachmawati Masrul Djalal Mhicya Utami R Miftah Irramah Mohamad Reza Mohamad Reza Muhammad Alhardi Nurdin Nelmi Silvia Nelwati Nelwati Netti Suharti Netti Suharti, Netti Nora Harminati Noverika Windasari Novialdi . Nur Afrainin Syah Nur Indrawati Lipoeto Nur Indrawaty Lipoeto Nurhajjah, Sitti Nursyafi, Fauzan Syarif Nurullah, Afi Putra Perdana Nuzulia Irawati Praja, Fathira Luthfi Nuresa Rahmadini Faricha Hakim Rahmi Ramadhani Raihan Eksa Dwi Tarisa Rana Zara Athaya Rauza Sukma Rita Resti Ayu Safitri Rikarni Rikarni Rima Semiarty Rina Gustia Rinang Mariko Riona, Vira Harisa Rony Rustam Roza Mulyana Roza Silvia Roza Silvia Saptino Miro Sari, Anisha Purnama Selfi Renita Rusjdi Sisca Dwi Yarni Siti Nurhajjah Sukri Rahman Syahrul, Muhammad Zulfadli Tessa Tamara Novhadi Titi Isnaini Tofrizal Trisno, Assyifa Azzahra Dwi Vetty Priscilla Vitresia, Havriza Wati, Westi Permata Yarni, Sisca Dwi Yohanna Eclesia Lumban Gaol Yulistini, Yulistini Yunia Habsari Yuniar Lestari Yuniarti Yuniarti Yusrawati Yusrawati Yustini Alioes Zaki Farhan Zakiya Zar'a Zelly Dia Rofinda Zurayya Fadila