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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Fitopatologi Indonesia Vegetalika Jurnal Sain Veteriner Jurnal Ilmu Pertanian Jurnal Penelitian Saintek Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Buletin Veteriner Udayana Jurnal Veteriner Jurnal Natur Indonesia Jurnal Pendidikan Matematika dan IPA Prosiding Seminar Biologi Bioedukasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Jurnal Sains dan Teknologi BUNGAMPUTI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Hutan Tanaman Jurnal Riset Ekonomi & Bisnis Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Biotropika Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Biotechnology Biota Biology, Medicine, & Natural Product Chemistry Planta Tropika Bioeksperimen: Jurnal Penelitian Biologi Majalah Obat Tradisional Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Scientiae Educatia: Jurnal Pendidikan Sains BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Ilmiah KOMPUTASI Jurnal Biodjati BIOTROPIC The Journal of Tropical Biology PROCEEDING ICBS Biosaintifika: Journal of Biology & Biology Education Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) jurnal Ilmu Pertanian (Agricultural Science) INSIST (International Series on Interdisciplinary Research) Jurnal Teknologi Pertanian Andalas Jurnal Riset Pendidikan Kimia (JRPK) Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal JKFT Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Akuntansi Food ScienTech Journal Sains dan Matematika International Journal of Environment, Engineering, and Education Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal of Environment, Engineering & Education Pasundan International of Community Services Journal (PICS-J) ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Proceeding Biology Education Conference Berkala Penelitian Hayati Comsep : Jurnal Pengabdian Kepada Masyarakat Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Filogeni: Jurnal Mahasiswa Biologi Jurnal Kedokteran Hewan Berkala Ilmiah Biologi Journal of Fisheries & Marine BIO-SAINS | Jurnal Ilmiah Biologi Indonesian Journal of Jamu

BUDI SETIADI DARYONO

Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia

Author-ID : 250513

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Molecular characters of melon (Cucumis melo L. ‘Kinaya’) using inter simple sequence repeat Budi Setiadi Daryono; Puti Hana Ramadhani; Esty Nidianti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.15997

Cultivar ‘Kinaya’ is a melon produced from breeding between ‘Sonya’ and ‘Kinanti’. This study on phenotypic and molecular characters is carried out to support ‘Kinaya’ as a superior cultivar. Our study aimed to analyze the genetic variation of the melon ‘Kinaya Bulat Kuning’ and ‘Kinaya Kuning Lonjong’ and its parental ‘Sonya’ and ‘Kinanti’. The research was conducted in the Greenhouse of Mutihan, D.I.Yogyakarta, and the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Quantitative characters were determined by morphometric comparisons, fruit weights, and the number of seeds. Qualitative characters included fruit color, fruit skin color, aroma, texture, and taste. The molecular characterization method was inter simple sequence repeat (ISSR) and included DNA isolation, spectrophotometry, amplification of DNA target using PCR, and visualization of DNA target. Molecular characters were analyzed using spectrophotometry and visualization of DNA bands by electrophoresis using the MVSP 3.1 program. PCR used four random primers such as UBC 807, 809, 810, and 812, which obtained 11 polymorphic and 12 monomorphic DNA bands with a polymorphism rate of 47.8%. It is known that the cultivar ‘Kinaya’ has a similarity of 72% with its inductees ‘Sonya’ and ‘Kinanti’.

Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’ Deris Trian Rahmandhias; Wiko Arif Wibowo; Aprilia Sufi Subiastuti; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17719

Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a horticultural plant that belongs to the Cucurbitaceae family with high public demand, however, local markets sometimes have limited supply. The existence of watermelon varieties that are not pest-resistant causes its production to be erratic. Therefore, plant breeding efforts are required to produce superior varieties through the stability test of plant characters. For watermelon to be certified as a new variety, it needs to possess a stable and adaptive character to various conditions. The F1 watermelon from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’ produces inole-shaped fruit, red flesh, and a sweet taste. Therefore, this study aims to determine the stability of the phenotypic character of watermelon F2 from crossing ♀ ‘Maduri’ with cultivar ♂ ‘Putri Delima’ and was conducted in Jamusan, Bokoharjo, Prambanan, Sleman, D.I.Yogyakarta fields from August to December. There are five samples of ripe watermelons that were selected randomly while their phenotypic characters were observed qualitatively and quantitatively. Each F2 watermelon character was compared to F1, and the quantitative analysis was conducted using one-factor ANOVA with a confidence level of 5%. The results of quantitative character analysis between F2 and F1 showed a P (P-value)> 0.05. Meanwhile, the results of qualitative observations of F2 watermelon showed different flesh and skin color, while the harvest time from F1 was caused by the segregation of heterozygous crosses. Therefore, it is necessary to select superior phenotypic characters as desired for the next breeding.

Application of Multiplex RT-PCR for Detection of Cucurbit-infecting Tobamovirus Budi Setiadi Daryono; Keiko T. Natsuaki
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) are seed borne viruses and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. To detect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in Bali-Indonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia and Cucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could be reported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses.Keywords : Cucurbit infecting Tobamovirus, multiplex RT-PCR, seed borne viruses

Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Budi Setiadi Daryono; Ganies Riza Aristya; Rina Sri Kasiamdari
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5

Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplified Polymorphic DNA Ajeng Kusumaningtyas Pramono; Budi Setiadi Daryono
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Magnaporthe oryzae B.Couch is a host-specific fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplified Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), finger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplified fragments.Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, finger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show host-specific specificity.Key words: Magnaporthe oryzae, RAPD, mating type

The genetic variations and relationship of Madura tobacco (Nicotiana tabacum L.) based on molecular characteristics Fitri Nadifah; Budi Setiadi Daryono
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Madura has at least 22 genotypes of local tobaccos (Nicotiana tabacum L.). This diversity could potentially produce new genotype of tobaccos with superior characters. However, information of the genetic diversity of Madura tobaccos is still limited. The aim of this study was to determine the genetic variation and relationship of 24 genotypes of Madura tobaccos with Random Amplified Polymorphic DNA (RAPD) analysis. In this research we were used 6 single primers for amplification: (OPA-18, OPB-12, OPB-14, OPC-1, OPC-8 and OPC-19) and 2 mixture primers ((OPB-12+OPC-8) and (OPC-1+OPC-19)). Genetic similarity and clustering was analyzed with Unweighted Pair Group Method Arithmetic (UPGMA) method with Numerical Taxonomy and Multivariate Analysis System (NTSYS) version 2.10 software. From this research we found that OPA18425, OPB12450, OPC8500, (OPC19+OPC1)550 and OPC8800 can be used as specific markers. Polymorphic bands percentage with mixture primers was relatively equal with single primers (<60%). The dendogram showed that Madura tobacco genotypes consist of 2 main clusters: cluster A (22 genotypes) and cluster B (2 genotypes: Bukabu Sa’ang and Prancak-95). Madura tobaccos had high genetic similarity between genotypes ranging from 0.80-1.00.

Application of Molecular Biology for Identification of Virus Resistance Gene in Melon Budi Setiadi Daryono
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Source of resistance to an Indonesia isolate of Cucumber mosaic virus (CMV-B2) in melon cultivarYamatouri has been reported. Moreover, Creb-2, a locus that confers resistance to CMV-B2 in Yamatouri hasbeen determined as a single dominant gene. To elucidate the resistance mechanism conferred by Creb-2 inmore detail, it is necessary to clone the Creb-2 gene and determine its molecular structure. One approach isby amplification and cloning of melon resistance gene analogs (MRGAs) based on degenerated PCR primersdesigned from conserved amino acids in the NBS-LRR motifs (P-loop, Kinase-2, and the GLPL) and Toll/Interleukin-1 receptor-like region (TIR). This study was aimed to identify and characterize the resistance geneanalogs from Cucumis melo L. cv. Yamatouri by employing polymerase chain reactions (PCR) as a molecularbiology tools with degenerate primers based on conserved motifs of cloned R genes. The application of molecularbiology such as DNA isolation, degenerate primers and PCR condition, cloning, sequencing, linkage analysisand mapping of resistance gene analogs to Creb-2 gene in melon will be widely discussed in this paper

Early detection of the orchid flowering gene PaFT1 in tobacco cells using a GFP reporter Sri Wahyuningsih; Muhammad Dylan Lawrie; Budi Setiadi Daryono; Sukarti Moeljopawiro; Soenghoe Jang; Endang Semiarti
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Here we describe a novel method of using green fluorescence protein (GFP) as a reporter gene for early detection of an integrated TDNA containing the orchid flowering gene, PaFT1 (Phalaenopsis aphrodite Flowering locus T1) in the tobacco genome. Functional assays that report the presence of exogenous DNA early in development are especially useful in plants where the desired phenotype is only apparent after long periods of vegetative growth. The objective of this study is to establish a method for detecting an inserted Phalaenopsis orchid flowering gene and examining its function in tobacco. The p35S::PaFT1 35S::GFP construct was introduced into Agrobacterium tumefaciens strain EHA101. Transformed tobacco leaves were cultured on MS medium with addition of 1 mgL-1 NAA+3 mgL-1 BAP+50 mgL-1 Kanamycin+300 mgL-1 timentin for selection. Results showed bright green GFP fluorescent signals in 11 out of 15 (73%) tobacco leaf cells at a 2month time point after transformation. GFP and PaFT1 fragments were amplified in genomic PCR using GFP and PaFT1 specific primers. The accumulated PaFT1 transcripts were observed in 3 monthold transgenic tobacco plants containing p35S::PaFT135S::GFP. Green florescence was observed only in the transgenic plants at the 5 monthold stage but not in the wild type controls.

ANALISIS FENOTIPE DAN PLOIDI TANAMAN MELON (Cucumis melo L.) HASIL PERLAKUAN EKSTRAK ETANOLIK DAUN TAPAK DARA (Catharanthus roseus [L] G. Don.) Nugroho Nofriarno; Budi Setiadi Daryono; Avia Purnama Saputri; Estiyani Indraningsih
Biota Vol 4 No 2 (2018): Jurnal Biota 2018
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v4i2.2061

Anti-mitotic agents such as colchicine have been used to induce polyploidy in various plants. On the other hand, vincristine and vinblastine are also antimitotic agent extracted from Periwincle (Catharanthus roseus [L] G. Don) were previously studied to produce autotetraploid on shallot tuber (Allium cepa L.). Therefore, in this study phenotype character and ploidy of muskmelon (Cucumis melo L.) produced by etanolic extract of periwikle leaves were determined. The effects of different concentration of etanolic extract of periwinkle leaves on polyploidy induction in muskmelon were examined. Melon seedling of two days old were immersed in 0.5%, 0.1%, and 0.05% for 8 hours. Then seedling was grown on the polybag and a drop of each concentration of periwinkle leaves’s etanolic extract was added into apical shoot. Melon seedling of ten days old were moved and cultivated and harvested on 60 days after cultivation. Phenotypic character such as: plant high, stem diameter, leaf area, fruit weight, fruit area around, flesh fruit thickness, skin fruit thickness, fruit horizontal diameter, fruit, vertical diameter, number of seeds, weight of 100 gram of seed, seed leght, seed width, and seed thickness were examined. The ploidy degree was determined by count of chromosome number root tips of second generation muskmelon sprout. Result of this study revealed that 0.05% etanolic extract of periwinkle leaves for 8 hours immersed is optimum concentration to induce autotetraploid muskmelon (4n=48). Autotetraploid phenotypic character of muskmelon produced by 0.05% etanolic extract of periwinkle leaves were generally bigger than control plants statistically significant in stem diameter, leaf area, and fruit horizontal diameter. The result also showed that the chromosome number of second generation autotetraploid muskmelon sprout was tetraploid (4n=48).

Perbanyakan Cissus quadrangularis L. dengan Stek Batang Siti Fatimah Hanum,Tri Warseno, dan Ema Hendriyani
Vegetalika Vol 2, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.2416

Cissus quadrangularis L. merupakan salah satu anggota suku Vitaceae yang memiliki khasiat obat. Salah satunya di masyarakat Kabupaten Buleleng, Bali tanaman ini dikenal dapat menyembuhkan ambeien dengan cara memakan batangnya. Karena manfaatnya itu maka Kebun Raya „Eka Karya‟ Bali-LIPI mengkoleksinya di Taman Usada (Koleksi Tanaman Obat). Namun ironisnya pertumbuhannya sangat lamban dan tidak sehat. Penelitian ini bertujuan untuk mengetahui pengaruh lokasi penanaman dan media tanam terhadap pertumbuhan stek batang Cissus quadrangularis L.. Penelitian ini dilaksanakan di Kebun Raya „Eka Karya‟ Bali-LIPI pada bulan Juni hingga Oktober 2011. Percobaan menggunakan Rancangan Acak Kelompok faktorial 3 x 5 dengan 3 ulangan. Parameter yang diamati adalah pertumbuhan vegetatif meliputi rata-rata jumlah daun, jumlah cabang, persentase berakar dan awal muncul tunas. Hasil yang diperoleh memperlihatkan pertumbuhan vegetatif C.quadrangularis L.. paling baik pada lokasi Pembibitan. Media tanam tidak memberikan pengaruh terhadap pertumbuhan vegetatif C.quadrangularis L..

Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anisa Parazulfa Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Aurantika, Rindu Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Bernardinus Pratama Budy Wiryawan Catur Ahda Darojatun Darmastuti Deris Trian Rahmandhias Desi Oktaviani Diah Rachmawati Diah Rachmawati DIAH RACHMAWATI DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Fadilah Husnun Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidayat, Madyan Akmal Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Indra Lesmana Irma Nofitahesti Ishak, Muhammad Alif Ismail Ismail Isna Mustafiatul Ummah Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Karmilah, Karmilah Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kristamtini Kristamtini, Kristamtini Kurniawan, Febri Yuda Kusnanda, Prima Sekti Latifah, Vida Rahma Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Muazam, Arif Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Nainggolan, Ananto Puradi Narzassi, Angga Bintang Nia Fararid Askar Niken Satuti Nur Handayani Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurcahyati, Vivi Indah Nurdilfa, Nurdilfa Nurhasanah, Anggun Diyan Ovami, Debbi Chyntia Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Pratiwi, Arini Dian Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo - Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putri Renata, Nellis Nadinda Putro, Karso Suryo Putu Ayu Damayanti Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Setyorini Widyayanti, Setyorini Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, â€ªTeuku Nanda Saifullah Sulaiman, â€ªTeuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaimanâ€¬, â€ªTeuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supriyadi Supriyadi Susamto Somowiyarjo Susilawati Susilawati Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz AsyÃ¢Â€Â™ari Hasbullah, Umar Hafidz AsyÃ¢Â€Â™ari Utin Elsya Puspita Utin Elsya Puspita Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Widyasari, Adristi Shafa Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yudhistira Nugraha Yuliara, I Made Yusuf, Adib Fakhruddin

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