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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos Biotika: Jurnal Ilmiah Biologi Bioeducation Journal JURNAL SAINS PERTANIAN EQUATOR Ilmu Administrasi Publik BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengajaran MIPA EDUSAINS ENGINEERING Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati TELKOMNIKA (Telecommunication Computing Electronics and Control) Biogenesis: Jurnal Ilmiah Biologi Jurnal AgroBiogen Jurnal Pengajaran Matematika dan Ilmu Pengetahuan Alam Formica Online Jurnal Matematika & Sains Techno: Jurnal Penelitian Jurnal Bioterdidik: Wahana Ekspresi Ilmiah Bioedukasi: Jurnal Pendidikan Biologi Journal of Mathematical and Fundamental Sciences JURNAL INTEGRASI PROSES Jurnal Pendidikan Biologi Indonesia Jurnal Inovasi Pendidikan IPA QUANTUM: Jurnal Inovasi Pendidikan Sains Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan REINWARDTIA BERITA BIOLOGI E-Dimas: Jurnal Pengabdian kepada Masyarakat Pedagogi Hayati International Journal of Science and Applied Science: Conference Series Jurnal DIDIKA: Wahana Ilmiah Pendidikan Dasar Titian Ilmu: Jurnal Ilmiah Multi Sciences Jurnal Biodjati Knowledge Engineering and Data Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika BIOEDUKASI Publik (Jurnal Ilmu Administrasi) Jurnal IPA Terpadu Journal of Natural Science and Integration Biosfer: Jurnal Pendidikan Biologi EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Bioma : Jurnal Biologi Indonesia Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Assimilation: Indonesian Journal of Biology Education Bio Educatio : The Journal of Science and Biology Education Jurnal BIOEDUIN : Program Studi Pendidikan Biologi Bioedukasi: Jurnal Pendidikan Biologi ABDIMAS SILIWANGI Lectura : Jurnal Pendidikan Paspalum: Jurnal Ilmiah Pertanian BIOSFER : Jurnal Biologi dan Pendidikan Biologi Jurnal Mangifera Edu JURNAL PENDIDIKAN MIPA Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Journal of Welding Technology Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Jurnal Pendidikan Teknik Mesin Undiksha BERNAS: Jurnal Pengabdian Kepada Masyarakat 3BIO: Journal of Biological Science, Technology and Management SIMBIOSA Indonesian Journal of Social Research (IJSR) Yumary: Jurnal Pengabdian kepada Masyarakat Jurnal Riset Teknologi Pencegahan Pencemaran Industri Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran JIPB (Jurnal Inovasi Pembelajaran Biologi) Proceeding Biology Education Conference Bioed : Jurnal Pendidikan Biologi El-Jughrafiyah : Jurnal Geografi dan Terapannya Jurnal Pendidikan Dasar dan Sosial Humaniora Journal of Comprehensive Science Jurnal Ilmiah Ilmu Terapan Universitas Jambi Biology and Education Journal (BaEJ) Acta Pedagogia Asiana Jurnal Penelitian Ekonomi Manajemen dan Bisnis Gunung Djati Conference Series Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) JIPI (Jurnal IPA dan Pembelajaran IPA) IBERS : Jurnal Pendidikan Indonesia Bermutu Biodik: Jurnal Ilmiah Pendidikan Biologi Jurnal Pendidikan & Pengajaran Reinwardtia Journal of Computers for Society Jurnal Pendidikan MIPA Jurnal Mahasiswa Sistem Informasi Galuh (JMSIG) IJIS Edu : Indonesian Journal of Integrated Science Education
Topik Hidayat
Universitas Pendidikan Indonesia
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PEMANFAATAN PENANDA trnL-F DALAM ANALISIS MOLEKULER UNTUK IDENTIFIKASI TUMBUHAN LANGKA SECARA IN SILICO Kocha, Santana; Hidayat, Topik; Hernawati, Hernawati
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 2 (2025): BIOTIKA DESEMBER 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i2.64272

Abstract

Penelitian ini bertujuan mengembangkan primer diagnostik berbasis penanda trnL-F secara in silico untuk mendeteksi tumbuhan langka. Biodiversitas mencakup variasi kehidupan di bumi, dari gen, spesies, hingga ekosistem. Semakin tinggi keanekaragaman hayati, semakin stabil dan produktif suatu ekosistem. Selain itu, variasi genetik membantu organisme beradaptasi terhadap perubahan lingkungan dan iklim. Menurut IUCN, lebih dari 40% spesies tumbuhan di dunia terancam punah akibat perubahan iklim, ketidakstabilan genom, deforestasi, dan aktivitas manusia. Gangguan ini dapat merusak fungsi gen penting dan mengurangi kemampuan adaptasi, membuat tumbuhan langka semakin rentan terhadap kepunahan. Maka dari itu upaya konservasi sangat dibutuhkan, namun identifikasi dan karakterisasi tumbuhan langka masih menjadi tantangan karena metode tradisional karena sering kurang akurat. Kelemahan penanda morfologi mendorong penggunaan penanda molekuler DNA. DNA barcode berbasis trnL-F memungkinkan identifikasi in silico secara cepat, akurat, dan non-destruktif. Teknik in silico PCR juga memungkinkan perancangan eksperimen secara efisien, murah, dan tanpa laboratorium. Sebanyak 30 sekuens DNA tumbuhan langka dari data IUCN dianalisis menggunakan perangkat lunak ClustalX, BioEdit, dan FastPCR. Hasil analisis ini dihasilkan 12 kandidat primer (6 forward dan 6 reverse). Uji in silico PCR terhadap 12 spesies langka menunjukkan satu pasangan primer terbaik, yaitu forward (1:F_255–277) dan reverse (1:R_355–375). Uji efektivitas terhadap 12 spesies non-langka menunjukkan spesifisitas 100% dengan hasil PCR negatif. Berdasarkan hal tersebut dapat diartikan bahwa temuan ini menunjukkan pasangan primer tersebut sangat potensial untuk deteksi dan konservasi tumbuhan langka secara efisien dan akurat.
In Silico Phylogenetic Analysis of Lamiaceae Based on ITS, matK, and rbcL DNA Barcodes Endlessa, Chayra; Hidayat , Topik; Sriyati , Siti
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 1 (2026): Vol 8 No 1 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.1.4

Abstract

Lamiaceae, widely used as herbal medicine, is increasingly vulnerable to adulteration driven by market demand, compromising product safety and efficacy. Prevention is challenging due to morphological similarities; thus, DNA-based phylogenetics offer an alternative for accurate species authentication. However, Lamiaceae phylogenetics remain complicated by inconsistencies between morphological and DNA data. This study reconstructed Lamiaceae phylogeny using partial ITS, matK, and rbcL barcodes to evaluate their potential application in species authentication and adulteration prevention. Sequences for 52 species across 11 genera (Spathodea campanulata: outgroup) were obtained from NCBI GenBank, aligned, and trimmed. Four maximum parsimony (MP) trees were constructed in MEGA 11 (three single-barcode, one concatenated). The concatenated dataset was also analyzed by maximum likelihood (ML). Tree robustness was evaluated with bootstrapping, consistency index (CI), and retention index (RI). matK had the longest mean sequence (785.6 bp), rbcL the highest homology (83.5%), and ITS the most parsimony-informative sites (40.3%). MP trees exhibited moderate homoplasy (mean CI = 0.63) but strong synapomorphic signal (mean RI = 0.83). Individual barcodes produced similar genus groupings, yet misplaced several species. Concatenation corrected these positions across MP and ML trees, resolving six robust monophyletic clades (bootstrap >70%), broadly consistent with earlier phylogenies: Callicarpa; Scutellaria; Clerodendrum, Lamium, and Stachys; Salvia; Thymus, Origanum, and Mentha; Orthosiphon and Ocimum. Topological discrepancies with prior studies likely reflect differences in barcode choice and taxon sampling. Concatenated barcodes improved phylogenetic resolution in Lamiaceae, producing clades that identify potential adulterants and guide DNA marker development for species authentication and adulterant detection.
Potential of The Citizen Science Project (CSP) to Support Biodiversity Learning in Indonesia Gusti, Utari Akhir; Hidayat, Topik; Sriyati, Siti; Ananda, Gheri Febri
Bioeducation Journal Vol 9 No 2 (2025): Bioeducation Journal
Publisher : Universitas Negeri Padang Address: Biology Education Study Program Faculty Mathematics and Natural Science (FMIPA) Jl. Prof. Dr. Hamka Air Tawar Barat, Padang-West Sumatera-Indonesia Telp. +62751-7057420 - Fax.+62751-7058772 - Ph. +6281363229286

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bioedu.v9i2.527

Abstract

Conservation of biodiversity in Indonesia is very important. This condition is supported by the high diversity owned but very minimal conservation efforts. Collaborative action between local communities and scientists in learning is needed to support conservation through education. Therefore, it is important to analyze the potential of citizen science projects in biodiversity lessons. This study aims to investigate the potential of citizen science projects and the condition of biodiversity learning in schools. This research is classified as descriptive qualitative. The sampling technique used random sampling with data collection using interviews, questionnaire distribution, and literature study. The results obtained show that citizen science projects have great potential in supporting biodiversity learning in Indonesia with local knowledge owned by indigenous peoples. The results are reinforced by data from students and teachers who agree to support citizen science project innovations in conscious, meaningful, and fun learning. This research reveals learning innovations that become alternatives in learning biodiversity in the success of learning outcomes in the form of conservation through education.
Analysis of students' conceptions of vertebrate diversity using concept maps Rifki Risma Munandar; Topik Hidayat; Yayan Sanjaya
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 6, No 3 (2024): October 2024
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v6i3.19347

Abstract

The concept of the characteristics of biodiversity is vital to be understood by students as a first step in managing and preserving the biodiversity. This study aimed to describe students' conceptions in vertebrate diversity and classification courses using concept maps. This research was conducted at one of the Biology Education Study Program Universities in Bogor, carried out in the even semester of 2023/2024, involving 42 participants. Data were collected through observation techniques with concept maps. The analysis process consisted of collecting information, simplifying information, presenting information, and drawing conclusions. The analysis results illustrate that students' conceptions of classification systematics, variability, phylogenetics, and preserving diversity and the environment show that they know the concept by 40.83%. The concept of the role of diversity in human life experienced the highest conception understanding of 54.61%. Students experienced many misconceptions about the concepts of characteristics, anatomy and morphology, genomes, and variability of biodiversity by 36.16%. Then 52.77% of students not knowing the concept of preserving diversity and the environment. The results of the concept map illustrate that most students still experience misconceptions and not knowing the concept of vertebrate diversity in detail. Therefore, training and strengthening concepts for students in classifying vertebrate animals are needed.Abstrak. Konsep mengenai karakteristik makhluk hidup penting untuk dipahami oleh mahasiswa sebagai langkah awal dalam mengelola dan melestarikan keanekaragaman makhluk hidup. Tujuan penelitian ini untuk mendeskripsikan konsepsi mahasiswa pada mata kuliah keanekaragaman dan klasifikasi vertebrata menggunakan peta konsep. Penelitian ini dilakukan di salah satu Perguruan Tinggi Prodi Pendidikan Biologi di Bogor, dilaksanakan pada semester genap tahun 2023/2024 dengan melibatkan 42 orang partisipan. Data dikumpulkan melalui teknik observasi dengan peta konsep. Proses analisis terdiri dari pengumpulan informasi, penyederhanaan informasi, penyajian informasi, dan penarikan kesimpulan. Hasil analisis menggambarkan bahwa konsepsi mahasiswa tentang sistematika klasifikasi, variabilitas, filogenetik dan konsep pelestarian keanekaragaman makhluk hidup dan lingkungan menunjukkan tahu konsep sebesar 40,83%. Konsep mengenai peranan keanekaragaman makhluk hidup bagi kehidupan manusia mengalami pemahaman konsepsi paling tinggi sebesar 54.61%. Mahasiswa banyak mengalami miskonsepsi pada konsep karakteristik, anatomi dan morfologi, genom, dan variabilitas makhluk hidup sebesar 36.16%. Kemudian 52.77% mahasiswa tidak tahu konsep pelestarian keanekaragaman makhluk hidup dan lingkungan. Hasil peta konsep menggambarkanan bahwa sebagian besar mahasiswa masih mengalami miskonsepsi dan tidak tahu konsep mengenai konsep keanekaragaman vertebrata secara detail. Oleh karenanya diperlukan latihan dan penguatan konsep bagi mahasiswa dalam klasifikasi hewan vertebrata.
Triple Helix Collaboration in the Creation and Development of Regional Excellent Products by the Hand-Painted Batik Craftsmen Group Handoko, Pryo; Kurnia, Nina; Tania, Reni; Bahtiar, Rizal; Hidayat, Topik
Publik : (Jurnal Ilmu Administrasi) Vol 14, No 2 (2025): December 2025
Publisher : Universitas Muhammadiyah Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31314/pjia.14.2.457-469.2025

Abstract

Banten Province ranks 7th out of 10 provinces with the most batik industries in Indonesia. This position indicates that Banten batik has significant economic and cultural potential. However, the development of hand-drawn batik still faces various obstacles, including fluctuations in raw materials, limited marketing, a decline in the number of artisans, low skills in motif and dye development, and limited capital. However, Banten batik has historical uniqueness because its motifs are inspired by cultural artifacts and the history of the Banten Sultanate. The complexity of these issues and potentials demands the involvement of various actors and sectors. This research aims to identify triple-helix collaboration patterns among government, academic, and business actors in the creation and development of regional batik flagship products. The method used is a qualitative approach with descriptive analysis. The research results show that inter-sectoral synergy plays an important role in enhancing production capacity, design innovation, access to capital, and market expansion, thereby strengthening the competitiveness of Banten batik as a regional flagship product.
Co-Authors A. Ch. Likadja, Jeffry A.A. Ketut Agung Cahyawan W Aa Juhanda Abd. Rasyid Syamsuri Abdull Rahim Mohd Yusoff Abdur Rasyid, Abdur Adelia Aryani Putri Adhi Yuniarto Adi Pancoro Adi Pancoro Adi Rahmat Aditya Suganda Aditya Suganda, Aditya Adriana Hiariej, Adriana Agus Sutanto Aini, Via Amalianneisha Rafadewi Andhanatami Putri Ana Ratna Wulan Ananda Yhuto Wibisono Putra Ananda, Gheri Febri Annisa Salsyabila Rahmi Any Fitriani Ari Widodo Aris Sunandar Aryanti, Fitri Astry Agusthina Muchtar Azmi Aris Bahtiar, Rizal Bambang Supriatno Cahyaningrum, Mahmudah Nur Cristanti, Widya Dadang Sudirno Damayanti, Anggia Fitri Damayanti, Elok Dede Salim Nahdi Della Frisca Damayanti Denia Dwi Citra Resmi Dhiyassalam Imam Anshori Ismanto Diah - Kusumawaty Dian Din Yati Diardy Shauman Rachmatan Didik Priyandoko Dina Karina Islami Dina Mariana Dindin Nasrudin Donna Karolina Br Surbakti Dwi Susilaningsih DWI SUSILANINGSIH Dwicahya Supriatman, Rian E. Derozari, Petrus E. Ermayanti Ekajaya, Renandy Kristianlie Ekaputri, Rendi Zulni Eko Budi Raharjo Eko Budi Raharjo, Eko Budi Endlessa, Chayra Eni Nuraeni Euis Erlin Fauzi Akbar Anugrah Fawzy Muhammad Bayfurqon Feni Oktaviani FENNY MARTHA DWIVANY Fidela Zhafirah Fitri Aryanti Fitri Aryanti Fitriani Nurpratiwi Susanto Fransisca Sudargo Fransisca Sudargo Tapilouw Fransisca Sudargo Tapilouw Fransisca Sudargo Tapilouw Fransisca Sudarto Tapilouw Ghullam Hamdu Giasintha Stefani Gusti, Utari Akhir Hafsah Hafsah Hana Gardenia Mahbubah Handoko, Pryo Hani Susanti Hani Susanti, Hani Haris Abizar Hayat, Muhammad Syaipul Helmi Helmia Tasti Adri Hernawati Hernawati Homdijah, Oom Siti Husna Nugrahapraja I Gusti Bagus Wiksuana I NYOMAN RAI Ika Adhani Sholihah Ilhami, Aldeva Imam Nugroho Indri Rachmitasuci Ipin Aripin Ipin Aripin Ipin Aripin Ipin Aripin, Ipin Iqbal Syaichurrozi Irmayanti Irmayanti, Irmayanti Jayawarsa, A.A. Ketut Karlia Meitha Kelana, Himalaya Wana Ketut Wikantika Khamidun, Mohd Hairul Kocha, Santana Koosbandiah Surtikanti, Hertien Kurnia, Nina Kurniawan, Iwan Setia Kusdianti Kusdianti Kusdianti Kusdianti Kusnadi Kusnadi Kusnadi Kusnadi Kusnadi Kusnadi Kusnadi Kusumawaty, Diah - Kwabena, James Lala Septem Riza Lia Lutianasari Lia Lutianasari Listia Andriani Maftuha, Mahda Rizqina Mahbubah, Hana Gardena Mahmoud Fahsi Manullang, Wahyu Efendi Meilia Gemilawati Miftakhul Bakhrir Rozaq Khamid Motomi - Ito Motomi - Ito, Motomi - Mr Amprasto Mr Sjaeful Anwar Mrs Sariwulan Diana Mu'aziyah, Siti Eneng Sururiyatul Muhammad Fakhri Basyir Muhammad Syaipul Hayat Muhammad Syaipul Hayat Nahadi Nahdiyati, Kartika Najira Natadiwijaya, Ismail Fikri nengsih juanengsih Nengsih Juanengsih Ningrum, Siti Ratu Rahayu Nisrina Sukriandi Nissa Rachmawati Nono Sutarno Nono Sutarno Novi Sofia Fitriasari Novita Evelyn, Teressa Nur Hamidah Nur Hamidah, Nur Nur'aeni, Epon Nurani Rahmawati, Dini Nurfauziah, Ade Nurhayati, Ai Siti Nurlela Nurlela Nurul Fadhilah, Nadia Nururrahmani, Azmah Nuryani Rustaman Nuryani Rustaman Nuryani Rustaman Nuryani Rustaman Nuryani Y Rustaman Nuryani Y Rustaman Nuryani Y. Rustaman Nuryani Y. Rustaman Nuryani Y. Rustaman Nuryani Yogipratama Rustaman Ono Rokhadhitomo Pancoro, Adi - Pangsuma, Nisa Pisca Hana Marsenda Purnamaulida Pratiwi Putra, Armansyah Putri Yunitha Wardiny Putri, Meirin Dwiningtyas R. Riandi, R. Rafi Muhammad F Rahma Widyanti, Nadya Rahman, M Ammar Fadhlur Rahmawati, Rima Aulia Ratni Purwasih Reni Tania, Reni Resmi, Denia Dwi Citra Riandi Riandi Riandi Ridwan - Rifki Risma Munandar Rifki Risma Munandar Rini Marwati Rini Nuroni Awaliyah Rini Nuroni Awaliyah Rini Solihat Risky Ayu Kristanti Risma Munandar, Rifki Rizky Nadhif Nandana Rosinta Septiana, Rosinta Rosyda, Miftahurrahma Sa'diatul Fuadiyah Salsabila, Amalia Putri Samah, Khyrina Airin Fariza Abu Santi Sri Rahayu Prajayanti sarbino . Sariani, Novita Setiasih Setiasih, Setiasih Setiono Sholihah, Ika Adhani Sidiq, Maulana Sihombing, Maria Engzelita Siregar, Herbert Siti Sriyati Soesy Asiah Soesilowaty Sofi Rahmania Solihat, Syifa Sri Anggraeni Sri Redjeki Sri Redjeki Sri Redjeki Sri Redjeki Sriyati , Siti Stevia Ladisa Suandana, Nana SUHIRMAN SUHIRMAN Sumiyati Sa'adah Sungkono Sungkono Supandi, Achmad Surtikanti Hertien Koosbandiah, Surtikanti Sururi, Zaki Fahreza Susbiyanto, Susbiyanto Syamsiar, Syamsiar Sylva Sagita Taufik Rahman Taufik Rahman Tengku Idris, Tengku Tiara Putri Hendriani Tomi Hidayat Tomohisa - Yukawa Tomohisa - Yukawa, Tomohisa - Tony Hadibarata, Tony Tony Hadibrata Tuszie Widhiyanti Tyas Farrah Dhiba Utari Akhir Gusti Visi Tinta Manik Vitta Yaumul Hikmawati Wahyu Surakusumah Wawan Setiawan Wida Herlina Widi Purwianingsih Win Heri Sarfudin Yayan Sanjaya Yayan Sanjaya YAYAN SANJAYA Yogi Yogi Yudi Prasetyo Yuyun Maryuningsih Yuyun Maryuningsih Zain, Muhammad Iqbal Zainal Arifin, Muhammad