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All Journal Media Kedokteran Hewan Journal Of Vocational Health Studies Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Darmabakti Cendekia: Journal of Community Service and Engagements Dinasti International Journal of Education Management and Social Science Airlangga Development Journal Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Assyarikah : Journal of Islamic Economic Business Jurnal Kedokteran Hewan Frontiers in Community Service and Empowerment Jurnal Penelitian Pendidikan Indonesia Central Community Development Journal Assyarikah: Journal Of Islamic Economic Business Jurnal Kesehatan Masyarakat

Sri Pantja Madyawati

Department Of Veterinary Medicine, Faculty Of Veterinary Medicine, Airlangga University, Surabaya

Author-ID : 3573491

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Prevalence and Risk Factor of Repeat Breeder Syndrome in Dairy Cows in KUD Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province Frida Aulya Arningdiah; Rahmatullah, Aldin Akbar; Cindy Ercha Aulia Putri; Mufasirin; Hermin Ratnani; Rimayanti; Sri Pantja Madyawati; Tita Damayanti Lestari; Muhammad Al-Syafiq bin Abdul Halim; Nuurin Ajrin Karim
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.232-242

This study aims to contribute valuable insights to readers and serve as a reference for dairy farmers looking to enhance their operations. By examining the prevalence and risk factors associated with repeat breeder syndrome in dairy cows within the Village Unit Cooperation (KUD) Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province, this study aims to aid in preventing such occurrences and ultimately improve dairy cow productivity. The survey method was employed, utilizing data sourced from cooperative and farmer records, as well as observations, interviews, and questionnaire responses. Analysis revealed that out of 50 farmers owning a total of 281 cows, 30 farmers possessed a combined total of 58 dairy cows classified as repeat breeders. Consequently, the prevalence rate of repeat breeders among farmers was found to be 20.64%. Descriptive analysis was conducted on the collected data, with additional SPSS Chi-Square (X²) analysis performed to identify risk factors associated with repeat breeders. These risk factors include breeder experience, knowledge of reproduction and estrus phases, communication with artificial inseminators and veterinarians, housing conditions, as well as factors related to livestock feed and water.

PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Nawangwulan, Tantri; Madyawati, Sri Pantja; Plumeriastuti, Hani; Sardjito, Trilas; Susilowati, Suherni; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.

PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Reisinta, Dinda; Paramita L, Widya; Hernawati, Tatik; Meles, Dewa Ketut; Madyawati, Sri Pantja; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aims to determined the effect of watermelon's inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats's spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat's spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.

PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Cesaria, Mayrena Ayu; Estoepangestie, A.T.Soelih; Susilowati, Suherni; Hernawati, Tatik; Madyawati, Sri Pantja; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan's Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.

DAMPAK PEMBERIAN ROTI AFKIR TERHADAP EFISIENSI REPRODUKSI SAPI PERAH Friesian Holstein DI WILAYAH KERJA KPSP SETIA KAWAN NONGKOJAJAR, PASURUAN Nurdianto S., Ricko; Hidanah, Sri; Madyawati, Sri Pantja; Samik, Abdul; wurlina, wurlina; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research is to know the impact of bread waste to efficiency reproduction of Friesian Holstein cow in regional working KPSP Setia Kawan Nongkojajar, Pasuruan by value Service per Conception (S/C), Conception Rate (CR), Days Open (DO). Method in this research is the dependent variable observed is the reproductive efficiency, the dependent variable as a supporter is the cow has given birth, the healthy dairy cow, the feed volume given. The data obtained is processed by using descriptive analysis to get the reproduction efficiency figure. The result of the research indicate that the value of Service per Conception (S/C) = 1.5 ± 0.69 times, Days Open (DO) = 95.6 ± 11.32 days and Conception Rate (CR) = 65% and Fertility Status (FS) = 72,4%. Results of this study can be concluded that bread waste provided by the breeder as an additional feed for dairy cow has no significant effect.

Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Masruro, Novia Alfi; Mulyati, Sri; Madyawati, Sri Pantja; Samik, Abdul; Ratnani, Hermin; Harijani, Nenny
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Efisiensi reproduksi pada sapi perah yang teridentifikasi Bakteri non spesifik dalam saluran reproduksi Qodri, Winda Nurul Lailatil; Mulyati, Sri; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Susilowati, Suherni; Tyasmingsih, Wiwiek
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.41-47

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collected from interview, questionaire, and direct observation at the beginning of the study. Detection of estrus was performed visually, followed by artificial insemination 12-18 hours later. Non-return to estrus was checked after 21 days. Artificial insemination was repeated up to three times on cows returning to estrous. Pregnancy was checked through rectal palpation 60 days after the last insemination. Pregnant cows were followed until calving. The results showed that the estrus rate, non-return rate (NRR), conception rate (CR), calving rate (CvR), services per conception (S/C), and days open (DO), were respectively 100%, 85%, 85%, 85%, 1.3, and 93 days. It could be concluded that the presence of non-specific bacteria in the reproductive tract of dairy cows did not affect the reproductive efficiency so that pregnancy and calving could still occur.

Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Ummaisyah, Winadya Reika; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Wurlina, Wurlina; Restiadi, Tjuk Imam; Wahjuni, Retno Sri
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS â‰¥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.

Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin Azwani, Nabila; Srianto, Pudji; Madyawati, Sri Pantja; Sardjito, Trilas; Suprihati, Endang
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.59-64

This case study aims to determine the effect of a combination of prostaglandin F2α (PGF2α) with Gonadotropin (PG-600), and human chorionic gonadotropin (hCG) on the estrus and pregnancy rates in Holstein Friesian (HF) cows with persistent corpus luteum (PCL). A total of 15 Holstein Friesian (HF) dairy cows aged 3-5 years with body condition score (BCS) 5-7 (scale 1-9) diagnosed with PCL, were injected with 7.5 mg PGF2α intra-vulvar submucosa. Cows were then divided into three treatment groups. Group P0 was not given the combination treatment, while P1 group was injected with a combination of 300 IU PG-600 and 150 IU hCG, meanwhile P2 group was was injected with a combination of 300 IU PG-600 and 300 IU hCG intra-muscularly. The PGF2α and PG-600 injections were carried out on the same day PCL was diagnosed. Meanwhile, the injection of hCG was carried out at the same time as the AI. The Pregnancy examination was performed 90 days after AI through rectal palpation. The results showed that all treated cows were estrus observed with the fastest onset of estrus was the P2 group, that was significantly different (p <0.05) compared to the P0 and P1 groups. All treated cows were diagnosed as pregnant. It can be concluded that the administration of PGF2α or its combination with gonadotropins in HF cows with PCL could result in 100% estrus and pregnancy rates.

Kualitas post-thawing semen domba Merino dalam bahan pengencer berbasis susu skim-kuning telur yang ditambah isolat crude protein Tirosine Kinase Widiantoro, Krisna; Madyawati, Sri Pantja; Sardjito, Trilas; Hernawati, Tatik; Triana, Indah Norma; Warsito, Sunaryo Hadi
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.39-45

This study was conducted to determine the effect of crude protein tyrosine kinase (PTK) isolates supplementation into skim milk-egg yolk based diluent to maintain the quality of Merino ram spermatozoa. Four ejaculates of two Merino rams were divided into two groups for the control group (P0): Merino ram semen was diluted in skimmed milk-egg yolk based diluent, and the treatment group (P1): Merino ram semen was diluted in skim milk-egg yolk based diluent contained crude PTK 1,597 mg/ml diluent. All diluted semen was equilibrated for 2 hours at 5 °C and filled into 0.25 mL French straws. The filled straws were placed on steel racks (Cooltop, Minitube) held in liquid nitrogen vapour for 10 minutes at –140 °C, immersed immediately in liquid nitrogen at –196 °C, and stored for 48 hours for later assessment. Post-thawed semen samples were evaluated for spermatozoa motility, viability, and morphological abnormality. The results showed that the spermatozoa motility of fresh semen of Merino ram was 82.5 ± 2.89, which was qualified for freezing. The post-thawing spermatozoa motility, viability, and morphological abnormalities of Merino ram in the P1 group were 34.11 ± 3.26%, 38.00 ± 3.00%, and 12.89 ± 4.54%, respectively. It were higher (p <0.05) than the control group of 24.44 ± 2.9%, 26.67 ± 3.32%, and 21.11 ± 3.02%. It was concluded that the addition of crude PTK isolates of 1.597 mg/ml skim milk-egg yolk diluent improved the quality of post-thawed spermatozoa of Merino ram.

Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abdullah Hasib Ach. Resa Fachrizi Aditya Irawan Aftabuddin Rz, Muhammad Agung Budianto Achmad, Agung Budianto Agustono, Bodhi Ahmad Hanif Azzam Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Anas Bad’rosana Prawindi Arimbi Aryaloka, Suhita Azwani, Nabila Badri Munir Sukoco Benjamin Christoffel Tehupuring, Benjamin Christoffel BUDI UTOMO Budi Utomo Candra Panji Asmoro, Candra Panji Cesaria, Mayrena Ayu Chrismanto, Dony Chrisnahutama, Adrian Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo Dikman, Dicky Mohammad Dimas Kunto Satrio Dito Herrys Dika Putra Djoko Legowo Emy Koestanti Sabdoningrum Endang Suprihati Epy Muhammad Luqman Erick Fajar Subhekti ERMA SAFITRI Erma Safitri Estoepangestie, A.T.Soelih Fachry Abda El Rahman Fajar Ramadhan Subiyantoro Fajarwati, Rini Fedik Abdul Rantam Fendy Suhariadi Feri Hendriyanto Frida Aulya Arningdiah Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hardaningtyas, Enggar Hariyono Hariyono Hendriyanto, Feri Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Hidanah, Sri Ika WAHYUNI IMAM MUSTOFA Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra Ira Sari Yudaniayanti Irawan, Adinda Riezki Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Jihan Pangestuningrum Kadek Rachmawati Kevin Mardotillah Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto, Kusnoto Lailiyah, Fahlevi Legowo, Djoko Leo Yosdimyati Romli Linda Sutanto Lucia Tri Suwanti, Lucia Tri Maryana Siska Silviani Masruro, Novia Alfi Masyitoh, Herlina Meles, Dewa Ketut Mirni Lamid Moh. Sukmanadi Mufasirin Muhammad Al-Syafiq bin Abdul Halim Muhammad Aulia Rahman Muhammad Ikmal Robih Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nawangwulan, Tantri Nenny Harijani Ni Luh Ayu Megasari Ni Made Sukartini Nidom, Chairul Anwar Nove Hidajati Nurdianto S., Ricko Nuurin Ajrin Karim octavia, Arinda Paramita L, Widya Paramita, Chici Ayu Pradana, Giffari Danindra Praja, Ratih Novita Prasetyo, Ivo Febrina Prastiya, Ragil Angga Pudji Srianto Purwono, Rudi Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Ratna Damayanti Reisinta, Dinda Renaldi, Nogi Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti . Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti Risanti Handayani Rochmah Kurnijasanti Rochmi, Siti Eliana Rudi Purwono Saputra, Hijrah Saputro, Amung Logam Sarmanu, Sarmanu Sayi Datur Rohmah Seagames Waluyo, Seagames Shifa Fauziyah Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mulyati Suherni Susilowati Sukma, Puspa Permata Sukmanadi, Mohammad Sunarso, Agus Suparto Wijoyo Supriyadi Supriyadi Suzanita Utama Tan, Chin Xuan TARZAN PURNOMO Tasya Apritalia Putri Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Ummaisyah, Winadya Reika Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wenny Nur Azizah Wibawati, Prima Ayu Widiantoro, Krisna Widiyatno, Thomas Valentinus Widjiati . Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yolifia Rahma Milenia Zahrorona, Amira Halimah

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