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Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas octavia, Arinda; Utomo, Budi; Madyawati, Sri Pantja; Suprayogi, Tri Wahyu; Sunarso, Agus; Hamid, Iwan Sahrial
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.65-71

Abstract

 This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.
Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature Retta, Ayun Tria Marga; Suherni Susilowati; Sri Pantja Madyawati; Tatik Hernawati; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.49-53

Abstract

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.
Combination of 5% Dextrose Ringer's solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature Ahmad Hanif Azzam; Dadik Rahardjo; Tatik Hernawati; Sri Pantja Madyawati; Budi Utomo
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.109-114

Abstract

This study aims to determine the motility and viability of kampung rooster (Gallus domesticus) spermatozoa in 5% Dextrose Ringer's solution as an extender with or without the addition of various egg yolk concentrations at 5ºC during storage. Semen was collected by massage in the morning. In this study, four types of extenders were used, i.e., 5% Dextrose Ringer's extender without egg yolk, 5% Dextrose Ringer's extender with 5, 7.5, and 10% egg yolk for T0, T1, T2, and T3, respectively. Then, 1000 IU Penicillin and 1 mg Streptomycin were added per mL extender. Fresh semen from each rooster was evenly divided into four volumes to be diluted 11 times in each group extender. Extended semen from all groups was stored at 5°C. Data were analyzed using ANOVA and Duncan's new multiple range test. The result showed a significant difference (p <0.05) between each treatment group for spermatozoa motility and viability. In conclusion, this study revealed that the addition of various concentrations of egg yolk into 5% Dextrose Ringer's solution as an extender maintained the motility and viability of kampung rooster spermatozoa.
Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke Maryana Siska Silviani; Moh. Sukmanadi; Rochmah Kurnijasanti; Sri Pantja Madyawati; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.130-136

Abstract

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.
Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

Abstract

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p  <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.
EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Pantja Madyawati, Sri; R, Rimayanti; W, Widjiati; Achmad, Agung Budianto
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2774

Abstract

Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF- dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.
EMPOWERMENT OF ELDERLY POSYANDU VOLUNTERS IN EARLY DETECTION OF HYPERTENSION DISEASE Hariyono, Hariyono; Sri Pantja Madyawati; Syifa Fauziyah; Candra Panji Asmoro; Teguh Hari Sucipto; Leo Yosdimyati Romli; Risanti Handayani
Frontiers in Community Service and Empowerment Vol. 2 No. 4 (2023): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v2i4.53

Abstract

The number of elderly populations in various countries continues to increase along with increasing life expectancy, so it is important to pay special attention to the health of the elderly, including early detection of hypertension. Posyandu volunters have good access to the elderly population because they are actively involved in community health activities. The aim of empowering elderly Posyandu volunters is to increase volunters' knowledge and skills in early detection of hypertension in the elderly. With adequate knowledge, volunters can provide better care and prevent more severe complications. The results of this research were that more than half had poor knowledge about early detection of hypertension before being given training (56%) and most of them had good knowledge about early detection of hypertension after being given training (63%). Empowering elderly Posyandu volunters has significant benefits. Skilled volunters can help increase elderly awareness of the importance of early detection of hypertension. Apart from that, volunters can provide assistance in regular health monitoring so that elderly people can take preventive measures in a timely manner..
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
Comprehensive analysis of economic, sociocultural, and environmental impacts on community well-being in tourist areas Saputra, Hijrah; Madyawati, Sri Pantja; Wijoyo, Suparto; Megasari, Ni Luh Ayu
Central Community Development Journal Vol. 5 No. 2 (2025): December 2025
Publisher : Privietlab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55942/ccdj.v5i2.527

Abstract

Tourism exerts a multidimensional influence on the well-being of communities in destination areas, encompassing economic, sociocultural, and environmental dimensions. This study aims to identify the key factors contributing to local community well-being and evaluate whether dependence on tourism has a measurable effect. A quantitative approach was employed using structured surveys involving 398 respondents from Karang Sidemen Village, Bali. Data were analyzed using descriptive statistics, Pearson’s correlation, multiple linear regression, and independent t-tests. The findings reveal that the economic impact is perceived as the most substantial contributor to well-being, followed by sociocultural and environmental aspects. The regression results indicate that economic, sociocultural, and environmental factors exert an equally significant influence (β = 0.333), whereas community attitudes, behavioral involvement, and tourism dependency show no statistical significance. Additionally, no significant difference in well-being was found between individuals reliant on tourism and those with alternative livelihoods (p = 0.506). These results underscore the importance of equitable and sustainable tourism development policies, emphasizing inclusive economic benefit distribution, cultural preservation, and responsible environmental management to ensure long-term community well-being and sustainability.
Workforce and Performance: Mastering Property Sales and Enhancing Marketing Skills During the COVID-19 Pandemic Feri Hendriyanto; Rudi Purwono; Sri Pantja Madyawati; Fendy Suhariadi
Dinasti International Journal of Education Management And Social Science Vol. 5 No. 6 (2024): Dinasti International Journal of Education Management and Social Science (Augus
Publisher : Dinasti Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/dijemss.v5i6.3131

Abstract

The property sector contributed 2.77% to Indonesia's Gross Domestic Product in 2019,. However, 2020 proved to be a challenging year globally due to the outbreak of COVID-19, which significantly disrupted various sectors, including the global economy. The extent of financial losses worldwide due to the pandemic has not been precisely quantified. This study explores the impact of the pandemic on the property market, specifically focusing on workforce dynamics, performance metrics, effective property sales strategies during the pandemic, and the enhancement of marketing competencies. Employing a qualitative, descriptive-analytical approach, this research concludes that exceptional property sales during the pandemic require rigorous effort, high performance, and advanced marketing skills. Competence, diligence, and performance are crucial in enhancing the capabilities of individual salespersons and, consequently, boosting organizational profits. This study highlights that the diligent and performance-oriented behaviors of salespersons are pivotal for acquiring and applying knowledge in sales scenarios, which are essential for navigating the complexities of the pandemic-affected market. The resilience shown by property salespersons in terms of time dedication and sustained effort amidst failures underscores their critical role in sustaining business operations during unprecedented times.
Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abdullah Hasib Ach. Resa Fachrizi Aditya Irawan Aftabuddin Rz, Muhammad Agung Budianto Achmad, Agung Budianto Agustono, Bodhi Ahmad Hanif Azzam Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Anas Bad’rosana Prawindi Arimbi Aryaloka, Suhita Azwani, Nabila Badri Munir Sukoco Benjamin Christoffel Tehupuring, Benjamin Christoffel BUDI UTOMO Budi Utomo Candra Panji Asmoro, Candra Panji Cesaria, Mayrena Ayu Chrismanto, Dony Chrisnahutama, Adrian Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo Dikman, Dicky Mohammad Dimas Kunto Satrio Dito Herrys Dika Putra Djoko Legowo Emy Koestanti Sabdoningrum Endang Suprihati Epy Muhammad Luqman Erick Fajar Subhekti ERMA SAFITRI Erma Safitri Estoepangestie, A.T.Soelih Fachry Abda El Rahman Fajar Ramadhan Subiyantoro Fajarwati, Rini Fedik Abdul Rantam Fendy Suhariadi Feri Hendriyanto Frida Aulya Arningdiah Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hardaningtyas, Enggar Hariyono Hariyono Hendriyanto, Feri Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Hidanah, Sri Ika WAHYUNI IMAM MUSTOFA Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra Ira Sari Yudaniayanti Irawan, Adinda Riezki Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Jihan Pangestuningrum Kadek Rachmawati Kevin Mardotillah Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto, Kusnoto Lailiyah, Fahlevi Legowo, Djoko Leo Yosdimyati Romli Linda Sutanto Lucia Tri Suwanti, Lucia Tri Maryana Siska Silviani Masruro, Novia Alfi Masyitoh, Herlina Meles, Dewa Ketut Mirni Lamid Moh. Sukmanadi Mufasirin Muhammad Al-Syafiq bin Abdul Halim Muhammad Aulia Rahman Muhammad Ikmal Robih Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nawangwulan, Tantri Nenny Harijani Ni Luh Ayu Megasari Ni Made Sukartini Nidom, Chairul Anwar Nove Hidajati Nurdianto S., Ricko Nuurin Ajrin Karim octavia, Arinda Paramita L, Widya Paramita, Chici Ayu Pradana, Giffari Danindra Praja, Ratih Novita Prasetyo, Ivo Febrina Prastiya, Ragil Angga Pudji Srianto Purwono, Rudi Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Ratna Damayanti Reisinta, Dinda Renaldi, Nogi Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti . Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti Risanti Handayani Rochmah Kurnijasanti Rochmi, Siti Eliana Rudi Purwono Saputra, Hijrah Saputro, Amung Logam Sarmanu, Sarmanu Sayi Datur Rohmah Seagames Waluyo, Seagames Shifa Fauziyah Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mulyati Suherni Susilowati Sukma, Puspa Permata Sukmanadi, Mohammad Sunarso, Agus Suparto Wijoyo Supriyadi Supriyadi Suzanita Utama Tan, Chin Xuan TARZAN PURNOMO Tasya Apritalia Putri Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Ummaisyah, Winadya Reika Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wenny Nur Azizah Wibawati, Prima Ayu Widiantoro, Krisna Widiyatno, Thomas Valentinus Widjiati . Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yolifia Rahma Milenia Zahrorona, Amira Halimah