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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Ilmu dan Teknologi Kelautan Tropis Hemera Zoa Media Veteriner Majalah Ilmiah Peternakan Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Reproduksi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology ZOO INDONESIA Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal ARSHI Veterinary Letters Jurnal Kedokteran Hewan Current Biomedicine

Arief Boediono

Bogor Agricultural University

Author-ID : 232094

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium ARIEF BOEDIONO; YULNAWATI YULNAWATI; MOHAMAD AGUS SETIADI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa

Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender . Yulnawati; Muhammad Gunawan; Hera Maheshwari; Muhammad Rizal; . Herdis; Arief Boediono
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied. The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms. The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively. And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.

Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes . WIDJIATI; ARIEF BOEDIONO; SUTIMAN BAMBANG SUMITRO; AUCKY HINTING; . AULANI’AM; TRINIL SUSILOWATI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.

The Side Effect of the Melastoma malabathricum L Ethanol Extract on the Gonad Maturation of Female Orange Mud Crab (Scylla olivacea) Nuril Farizah; Muhammad Zairin JR; Latifah Kosim Darusman; Arief Boediono; Muhammad Agus Suprayudi
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

The medicinal plants in aquaculture are currently being studied quite extensively by researchers. Medicinal plants can act as an inducer or aphrodisiac and an antifertility agent in gonad maturation. The aimed of the experiment was to known side effects of the herb M. malabathricum L. on maturation process in female mud crab (S. olivacea). This study used 40 crabs with an average weight of 200-250g. The experiment consists of two treatment groups. The controls group (A) that received 100 μl of aquadest and group treatment (B) received 100 μl of 2 mg/g of ethanol extract M. malabathricum L. were studied. For 5, 10, 15, and 20 days of experiment, crabs were anesthetized and sacrified. The ovary was collect for GSI and histology. The end of experiment days (20 day), the hemolymph was collect to used ELISA assay. The result showed, significant differences in treatment group and control groups (p<0.05) in terms the macroscopic morphology of ovary, the mean GSI and the diameter of oocytes. Histological showed the influence of extract M. malabathricum L. on inhibited the ovary development compared with the control groups. Based on this results, it is concluded that ethanol extract of M. malabathricum L. leaves at a dose of 2 mg/g act as an antifertility agent for ovary maturation of S. olivacea.

Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.

Differentiation of Cardiomyocytes and Identification of Cardiac Conduction System Connexins Derived from Bone Marrow Mesenchymal Stem Cells of Macaca nemestrina Agus Harsoyo; Irma Herawati Suparto; Yoga Yuniadi; Arief Boediono; Dondin Sajuthi
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.337

Bone marrow mesenchymal stem cells have been widely used, because plasticity, specific surface markers, self-renewal to transform into various lineages including cardiomyocytes. Information about the connexin (Cx) cardiac conduction systems of the pigtail macaque (Macaca nemestrina) is limited. This study aimed to evaluate cardiomyocyte differentiation from bone marrow mesenchymal stem cells of pigtail macaques and to clarify the Cx cardiac conduction system. Bone marrow aspirates were obtained from the proximal humerus of four adult male pigtail macaques, collected into heparinized tubes, then centrifuged to obtain mononuclear cells that were isolated and cultured in an incubator. After these cells reached 70–80% monolayer confluency as homogeneous fibroblast-like cells, they were subcultured. On the second subculture passage, the cells were pelleted to extract the mRNA, which was analysed by reverse transcription–polymerase chain reaction, and then cultured for a third passage. Cells were positive for CD73 and CD105 and the reference gene glyceraldehyde-3-phosphate dehydrogenase, and negative for CD34 and CD45. Osteogenic, chondrogenic, adipogenic, and cardiomyocyte differentiation was confirmed based on specific staining. The pigtail macaque bone marrow mesenchymal stem cells can be isolated and subcultured. The transcription of genes and translation of proteins of the connexin cardiac conduction systems was successfully identified.

Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.

Keasaman Cairan Tubuh dan Rasio Kelamin Anak Domba Garut (Ovis aries) yang Diberi Kation-Anion Ransum yang Berbeda F Fathul; T Toharmat; I G Permana; A Boediono
Media Peternakan Vol. 31 No. 2 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.385 KB)

The objective of this research was to determine the effects of dietary cation-anion difference (DCAD) on acidity of ration, blood, vaginal mucous, and urine, number of fetus, number of offspring, and offspring sex ratio. The treatments were rations with DCAD -28, -18, 0, +14, and +32 meq which were offered to 15 ewes. This research used randomized complete block design. There were three groups, (I) ewes previously had twin female offsprings; (II) ewes previously had twin male offsprings; and (III) ewes previously had twin male and female offsprings. Results of this study showed that there were no differences observed for blood's pH, vaginal liquid's pH, offspring number, and offspring sex ratio. Increasing DCAD affected ration's pH, urine's pH, and number of fetus for each ewe. Blood's acidity was not affected by DCAD, but DCAD -28 meq reduced acid of blood, metabolic acidosis, and urine; the number of fetus and offspring. DCAD -28 meq might be anions excessive in the ration. DCAD 0 meq was relatively the best of ration because gave the normality of blood, metabolic, and urine, also relatively the highest of fetus and offspring. The offspring sex ratio related to vaginal mucose pH when artificial insemination was applied (r= 0.75) and ration's pH (r=0.47). Ewes previously had twin female lambs gave the number of fetus and offspring sex ratio were 2.00+0.71 and 0%, respectively. Ewes previously had twin male lambs gave the number of fetus and offspring sex ratio were 2.80+1.30 and 15.00+8.50%, respectively. Ewes previously had twin male and female lambs gave the number of fetus and offspring sex ratio were 1.60+1.14 and 61.75+31.50%, respectively.Key words: DCAD, pH, offspring sex ratio, lamb, ewe

Mineral Utilization in Rams Fed Ration Supplemented with Different Levels of Chromium, Calcium, and Cation-Anion Balances D Sudrajat; T Toharmat; A Boediono; I G Permana; R I Arifiantini; F Amir
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Chromium (Cr) is an essential mineral for ruminants. Its metabolism and interactions with other minerals has not been widely known. This experiment was designed to evaluate the utilization of minerals and growth of Garut ram fed ration supplemented with Cr and different Dietary Cation Anion Balance (DCAB) and Ca level. Dietary treatments, namely: R0 (Ration with DCAB+14); R1 (Ration with DCAB+14 + Cr 3ppm,); R2 (Ration with DCAB 0 + Ca); R3 (Ration with DCAB 0 + Cr 3 ppm + Ca), were allocated in twenty four of 1.5-2 years old Garut rams in a randomized block design. The results showed that Cr supplementation in rations containing different levels of Ca did not affect feed intake, body weight gain, and dry matter digestibility, but reduced the absorption of Cr and Ca of the low Ca diet. Supplementation of Cr had no effect on Cr, Ca, Zn, and Mg status in blood and semen of the rams. Level of Cr intake had negative correlation with Ca absorption and positive correlation with blood Cr levels. There is a positive relationship between level of Ca intake with Ca and Mg absorption and blood Ca and Zn levels. Intake of Cr and Ca was not related to the semen Cr and Ca levels.

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm Y Yulnawati; H Maheshwari; M Rizal; A Boediono
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo’, as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity.

Co-Authors . AULANI’AM . Herdis . Yulnawati A Winarto A.S. Satyaningtijas Abinawanto Adi Winarto Adisti Dwijayanti Adkhilni Utami Adkhilni Utami Adrian Situmeang Adrian Situmeang Adrian Situmeang Adrien Jems Akiles Unitly Adrien Jems Akiles Unitly, Adrien Agus Harsoyo Agus Harsoyo Agus Oman Sudrajat Agus Setiadi Ahmed, Ifty Al Azhar Al Mukhlas Fikri AL-AZHAR AL-AZHAR Alfred O. M. Dima Alif Iman Fitrianto Alif Iman Fitrianto Alimuddin Alkaustariyah Lubis Amrozi Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andri Maruli Tua Lubis Andri Maruli Tua Lubis Andriyanto A Andriyanto Andriyanto ANOM BOWOLAKSONO Arie Adrianus Polim AS Aku, AS Aucky Hinting Aulia Miftakhur Rahman Ayu Mulia Sundari Bambang Kiranadi Batara Sirait Bayu Rosadi Berry Juliandi BIBIANA W LAY Bibiana W Lay Boenjamin Setiawan Boenjamin Setiawan Budiariati, Vista Cahayadi, Sigit Daru Cece Sumantri Chairun Nisa Citra Noviana Cutnya’ Shaliran Nazlie (Alm) D Sudrajat Dedy D. Solihin Diah Nugrahani Pristihadi Dian Anggraini Djaswadi Dasuki Djoko Walujo Dody Dharmawan Trijuno Dondin Sajuthi Dwi Budiono Dwierra Evvyernie Dwiranti, Astari Elpita Tarigan Eni Kusrini EVY AYU ARIDA F Amir F Fathul Farid A. Moeloek Ferry Sandra Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Funahashi, Hiroaki H Maheshwari Hadi, Restu S. Handina Rakhmawati Handina Rakhmawati Harry Murti Harry Murti Harry Murti Harry Murti Hartanti Kusumaningtyas HERA MAHESHWARI Herdis . herdis herdis Heri Sujoko Heru Setijanto I G Permana I Ketut Mudite Adnyana I Ketut Suatha I Supriatna I Wayan Batan I.G. Permana Ichsan Ichsan Iis Diatin Iman Supriatna Indra Bachtiar Indra Kusuma Irma H Suparto Irma Herawati Suparto Irma Suryani Ita Djuwita Ita Djuwita ITA DJUWITA Ita Fauzia Hanoum, Ita Fauzia Ivan Sini Karisma Mardatillah Karisma Mardatillah Kartini Eriani KARTINI ERIANI KARTINI ERIANI Kartiwa, R. Angga Kelvin Yaprianto Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Krido Brahmo Putro Kusdiantoro Mohamad Kusumaningtyas, Hartanti Latifah Kosim Darusman Lea Tarliyah Lindiawati, Riris M Agus Setiadi M Fakhrudin M Imron M Rizal M. Haviz M.A Setiadi Madihah Madihah Maheshwari H Maman Surachman Mas Rizky A.A. Syamsunarno Maula, Yogi Nikmatul Miraprahesti, Retti N. Mohamad Fakhrudin Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Gunawan Muhammad Gunawan Muhammad Gunawan MUHAMMAD RIZAL Muhammad Rizal Muhammad Rizal Muhammad Rosyid Ridlo Muhammad Zairin JR Muhammad Zairin Jr. MULYOTO PANGESTU MULYOTO PANGESTU Muslim Muslim N M D Haq NASTITI KUSUMORINI Nastiti Kusumorini Nastiti Kusumorini Nining Handayani Nining Handayani Nining Handhayani Noer Muhammad Dliyaul Haq Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Noor RR Nurhayati, Retno Wahyu Nurhidayat - Nurhidayat Nurhidayat Nuril Farizah Nursanti, Risa Nuzulia, Nur Aisyah Prakoso, Nurul Muhammad Prasetyaningtyas, Wahono Puspitasari, Riris R I Arifiantini R Sumarmin, R Rachmat Herman Rahmaniyah, Wiwit Ridhani Rahmat Hidayat Rahminiwati, Min Ramadhan Sumarmin Rangga Setiawan Rangga Setiawan Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Resti Rahma Dianti Ridi Arif Rimayanti - Rini Widyastuti Riris L. Puspitasari Riris L. Puspitasari Rizal M Ronny Rachman Noor Salsabila, Cyntia Bella Sandy Qlintang SATRIYAS ILYAS Satya Gunawan Seiichi Watanabe Shofwal Widad Sigit Prastowo Siti Darodjah Rasad Sony H. Sumarsono SONY HERU SUMARSONO SONY HERU SUMARSONO Sony Heru Sumarsono Sri Catur Setyawatiningsih Srihadi Agungpriyono Subangkit, Mawar Sulistiono Sumantri C Sumarsono, Sony H. Sumarsono, Sony Heru Sundari, Ayu Mulia Supar - Supar . Sutarya Enus Sutiman Bambang Sumitro T adelina T R Wiradarya T Toharmat T. Suzuki T. Toharmat T. Toharmat TAKDIR SAILI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Jamaan Thomas Mata Hine TL Yusuf, TL Trevino A. Pakasi Tri Aprilliana Wulandari TRINIL SUSILOWATI Tutik Wrediati Tutik Wresdiyati Tutty Laswardi Yusuf Tutty Laswardi Yusuf Tuty Laswardi Yusuf Uswatun Hasanah Vincentia Maria Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin Wasmen Manalu Widjiati Widjiati, Widjiati Wildan Mubarok Wining Astini Wining Astini Wiwit Ridhani Rahmaniyah Y Yulnawati Yessie Widya Sari Yoga Yuniadi Yuhara Sukra Yuhara Sukra Yulnawati . Yulnawati Y YULNAWATI YULNAWATI Yundari, Yundari Yushinta Fujaya

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