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Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Abstract

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.
Koleksi Sel Telur dengan Teknik Laparoskopi untuk Produksi Embrio dan Transfer Embrio pada Domba Mohamad Agus Setiadi; Iman Supriatna; Arief Boediono
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheep.Keyword: laparoscopic, oocyte, embryo transfer, sheep
Pemaparan Gelombang Elektromagnetik Telepon Genggam pada Mencit (Mus musculus albinus) Periode Awal Kebuntingan Vincentia Maria; Kusdiantoro Mohamad; Arief Boediono
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.16 KB) | DOI: 10.29244/avi.2.1.36-41

Abstract

Peningkatan penggunaan telepon genggam diiringi dengan peningkatan kepedulian masyarakatterhadap bidang kesehatan dan keamanan terkait emisi gelombang elektromagnetik yang berasaldari telepon genggam. Penelitian ini bertujuan mengetahui tingkat keamanan paparan gelombangelektromagnetik telepon genggam melalui pengamatan terhadap jumlah implantasi dan anak lahirdengan menggunakan mencit sebagai hewan model. Dua puluh empat ekor mencit betina disinkronisasidan dikawinkan dengan mencit jantan dengan perbandingan jantan dan betina 1:1 (single mating).Pemaparan dilakukan dengan gelombang berfrekuensi 900 MHz selama 7 hari pertama setelah mencitkawin. Mencit betina dibagi ke dalam empat kelompok berdasarkan lamanya waktu paparan. Waktupaparan adalah satu, dua, dan empat kali per hari dengan masing-masing lama paparan 15 menit untukkelompok pertama, kedua, dan ketiga, sementara kelompok keempat sebagai kelompok kontrol tidakdiberikan paparan. Hasil penelitian menunjukkan tidak adanya pengaruh nyata lama paparan gelombangelektromagnetik telepon genggam terhadap jumlah implantasi dan anak lahir dari induk yang terpapar(P>0,05). Untuk seluruh kelompok, jumlah implantasi berkisar antara 8,66 sampai dengan 10,00 danjumlah anak lahir berkisar antara 10,00 sampai dengan 12,33; tidak berbeda nyata dengan nilai padakelompok kontrol. Berdasarkan hasil penelitian, lama paparan gelombang elektromagnetik yang berasaldari telepon genggam dalam penelitian ini masih berada dalam tingkat aman untuk mencit bunting.
Peripheral Blood Mesenchymal Stem Cells Isolated from Indonesia Long Tailed Monkey (Macaca fascicularis) Agus Harsoyo; Dondin Sajuthi; Arief Boediono; Yoga Yuniadi; Irma H Suparto
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.537 KB) | DOI: 10.29244/avi.6.2.56-69

Abstract

An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal stem cell (MSC) isolate sources and the impact of its concentration on the pheriperal blood mononucleous cells (PBMC) development has been conducted. Twelve male Mf were used in this experiment. Three different age groups (infant (A1), juvenil (A2) and adult (A3)) of the Mfs were compared as treatments. Isolate of pheriperal blood MSC were created by taking 1 ml, 5 ml or 10 ml the Mfs pheriperal blood, processed them into PBMC, counted, isolated, cultured, subcultured, pelleted, extracted for their messenger Ribonucleic Acid (mRNA). Reverse transcriptase - polymerase chain reaction (RT-PCR) were conducted to obtain complentary Deoxyribonucleic Acid (cDNA). PCR amplification were performed to look cluster differentiation (CD) of the MSC gene expression. Incomplete block design was used and the data were analysed using descriptive statistic and T-Test. The results showed that PBMC counted from infant, juvenil and adult were 6.78 – 7.28, 6.18 – 7.30, and 6.01 – 7.34 log cell, respectively. The subculture and pelleting cells were only obtained from A3 with positive 73, 90, 105 and negative 34, 45 CD markers. It is concluded that pheriperal blood of adult Mf can be utilized as MSC source.
Folikel Pre-Ovulatori Sapi PO Dara yang Distimulasi Menggunakan PMSG Dosis Rendah Krido Brahmo Putro; Amrozi .; Adi Winarto; Arief Boediono; Wasmen Manalu
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.1-7

Abstract

Penelitian ini bertujuan untuk mengetahui perkembangan folikel sapi PO dara yang distimulasi menggunakan PMSG dosis rendah (dosis non-superovulasi) dan perolehan folikel dominan pre-ovulatori sebagai informasi dasar untuk penerapan intrauterine programming pada sapi. Sapi PO dara berjumlah sembilan ekor dibagi ke dalam tiga kelompok dosis PMSG yaitu kontrol (NaCl 0.9% sebagai placebo), dosis PMSG 0.5, dan 1.0 IU/kg BB. Injeksi PMSG dilakukan pada awal gelombang folikel ke-2 berdasarkan perubahan dinamika ovari yang dikonfirmasi menggunakan USG diikuti injeksi PGF2α 48 jam kemudian. Perkembangan folikel sejak injeksi PMSG hingga terbentuknya pre-ovulatori folikel (POF) diamati dan dipetakan menggunakan USG. Jumlah POF tertinggi terdapat pada dosis PMSG 1.0 IU/kg BB (4.67+1.67) secara signifikan (P<0.05), sedangkan POF yang terbentuk pada dosis PMSG 0.5 IU/kg BB berjumlah satu, sama dengan kelompok kontrol (P>0.05). Meskipun jumlah POF yang terbentuk berjumlah satu, namun rataan diameter dan volume kelompok sapi dosis 0.5 IU/kg BB lebih besar 16.13% dan 57.14% secara berurutan dibandingkan kelompok kontrol, diikuti oleh dosis 1.0 IU/kg BB dengan nilai tertinggi secara signifikan (P<0.05). Berdasarkan hasil tersebut dapat diketahui bahwa PMSG 0.5 IU/kg BB dapat terkontrol sehingga dapat digunakan sebagai metode pendekatan intrautering programming pada hewan monotokus.
Analisis Proteomik Cairan Sinovial Sendi Domba: Efektivitas Metode dan Profil Protein Fungsional Kusdiantoro Mohamad; Wiwit Ridhani Rahmaniyah; I Ketut Mudite Adnyane; Mokhamad Fahrudin; Arief Boediono
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1046.317 KB) | DOI: 10.29244/avi.8.2.52-64

Abstract

Penelitian ini bertujuan untuk mengevaluasi metode deplesi dan digesi protein, serta menganalisis proteom cairan sinovial (SF) sendi pada domba sehat menggunakan kromatografi cair–tandem spektrometri massa. Cairan SF dikoleksi dari enam ekor domba garut betina, umur ±4 tahun, berat 35-40 kg, dan sehat secara klinis. Deplesi protein berkelimpahan tinggi dilakukan dengan metode spin column menggunakan TOP 12 dan metode proteospin, sementara digesi protein dengan tiga metode, yaitu digesi in solution, in gel, dan in-solution + filter-aided sample preparation (FASP). Metode yang terbaik kemudian dilanjutkan dengan fraksinasi peptida menggunakan ultra performance liquid chromatography. Metode deplesi proteospin dengan digesi protein in-solution + FASP merupakan metode terbaik berdasarkan nilai coverage dan sequest HT. Hasil analisis proteomik terkarakterisasi 52 protein pada database spesies domba. Anotasi gen ontologi menggunakan DAVID analysis menunjukkan bahwa protein-protein SF tersebut merupakan komponen sel terutama sebagai eksosom ekstraseluler, fungsi molekuler sebagai aktivitas inhibitor endopeptidase tipe-serin dan pengikat ion kalsium; serta proses biologis sebagai angiogenesis dan koagulasi darah atau pembentukan fibrin. Analisis KEGG pathway menunjukkan protein SF berperan utama pada jalur kaskade koagulasi dan komplemen.
hCG Priming Before Ovary Collection Increasing The Oocyte Quality In The Domestic Cat Karisma Mardatillah; Rini Widyastuti; Diah Nugrahani Pristihadi; Wahyudin; Sigit Prastowo; Asep Gunawan; Arief Boediono
Acta VETERINARIA Indonesiana 2021: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi...123-126

Abstract

Oocyte competence is a determining factor that influences the embryo development. Embryos produced in vitro have a reduced developmental competence than embryos produced in vivo. Therefore, human Chorionic Gonadotropin (hCG) injection was carried out to improve the quality of the oocytes. The objective of this study was to evaluate the effect of ovarian stimulation with hCG before ovary collection on oocyte quality in the domestic cat. Oocyte donors were either 1) treated with a single dose of 200 IU hCG four days before ovary collection (hCG group), or, 2) no treatment before ovary collection (control group). The oocytes were collected by the slicing method. Immature cumulus oophorus complexes (COCs) from both groups were pooled and matured in vitro for 24-26 hours. Then mature oocytes were fertilized with epididymal sperm and cultured in vitro for seven days. The results study showed that the number of the dominant follicle (DF) and the number of COCs in the hCG group was higher than the control group in right and left ovaries (p<0.05). The morulae and blastocyst rates from cleavage embryos were 88% and 75%, respectively. These results demonstrate that hCG priming of oocytes donors before ovary collection improve oocyte quality.
Aktifitas dan pola makan, minum dan memamah biak kancil (Tragulus javanicus) di kebun binatang Ragunan Jakarta dan Surabaya Nurhidayat Said; Adi Winarto; Arief Boediono; Ita Djuwita; Chairun Nisa; Tutik Wrediati; Heru Setijanto; Mohamad Fakhrudin
Hemera Zoa Vol. 77 No. 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.711 KB)

Abstract

Telah dilakukan penelitian pada kancil (Tmgulus javanicus) di Kebun Binatang Ragunan - Jakarta dan Kebun Binatang Wonocolo - Surabaya untuk mendapatkan jenis makanan yang disukai, dan aktifitas makan, minum dan ruminasi dari kancil yang dikandangkan.Pada penelitian ini, disediakan 10 jenis makanan dan empat jenis diantaranya yaitu pisang, kacang panjang, kangkung dan pepaya lebih disukai. Aktifitas makan dilakukan pada siang dan malam hari, dan pada saat istirahat kancil melakukan aktifitas ruminasi. Perilaku makan dan ruminasi ini berbeda dengan perilakunya di habitas asalnya. Sedangkan aktifitas minum, jarang dilakukan, hal ini mungkin disebabkan oleh tingginya kadar air di dalam makanan yang disediakan. Aktifitas makan dari kancil yang dikandangkan telah berubah menjadi diurnal dan nokturnal.
PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
PCS-16 Determaining Surgical Method by Meniscectomy Induction on Garut sheep (Ovis aries) for early stage of Osteoarthritis Handina Rakhmawati; Adrian Situmeang; . Nurhidayat; Andri Maruli Tua Lubis; Harry Murti; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.044 KB)

Abstract

Osteoarthritis (OA) is the most common joint disease that cause of pain and disability by various factors such as advanced age, obesity, trauma, and arthritis disease. These factors affect by degeneration of the cartilage surface, leading to loss of matrix include proteoglycan osteophyte formation, subcondral and synovial membrane affected. In the healthy joint, meniscus, articular cartilage, subchondral bone, and synovial membrane provide support to the joint. The meniscus is an important load bearing structure and has nutritive as well as lubricating properties in the knee joint as well (Little et al. 2010).Animal models are research materials that can be used in studying potential pathogenesis and therapy in various diseases in humans. Sheep are commonly large animal model of OA because of the availability, ease of handling, and have a similarities with humans in size and structure of joint. In the development of science, sheep can be used as an animal model in studying the pathogenesis of diseases in human orthopedics studies such as joints, ligaments, and bones. Garut sheep is an Indonesian germplasm indigenous that has the structure, density, and size of joint anatomy that are similar in human joints rather than other small animals. This is the basis of the utilization of Garut sheep as an animal model in human orthopaedic. (Little et al. 2010; Gregory et al. 2012).The aim of this study was to identify and analyze the determining surgical method by meniscectomy induction on Garut sheep with 8 weeks post meniscectomy observation for early stage of OA.
Co-Authors . AULANI’AM . Herdis . Yulnawati A.S. Satyaningtijas Abinawanto Abinawanto Abinawanto Adi Winarto Adisti Dwijayanti Adkhilni Utami Adkhilni Utami Adrian Situmeang Adrian Situmeang Adrian Situmeang Adrien Jems Akiles Unitly Adrien Jems Akiles Unitly, Adrien Agus Harsoyo Agus Harsoyo Agus Oman Sudrajat Agus Setiadi Ahmed, Ifty Al Azhar Al Mukhlas Fikri AL-AZHAR AL-AZHAR Alfred O. M. Dima Alif Iman Fitrianto Alif Iman Fitrianto Alimuddin Alimuddin Alkaustariyah Lubis Amrozi Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andri Maruli Tua Lubis Andri Maruli Tua Lubis Andriyanto A Andriyanto Andriyanto ANOM BOWOLAKSONO Arie Adrianus Polim AS Aku, AS Aucky Hinting Aulia Miftakhur Rahman Ayu Mulia Sundari Bambang Kiranadi Batara Sirait Bayu Rosadi Berry Juliandi BIBIANA W LAY Bibiana W Lay Boenjamin Setiawan Boenjamin Setiawan Budiariati, Vista Cahayadi, Sigit Daru Cece Sumantri Chairun Nisa Citra Noviana Cutnya’ Shaliran Nazlie (Alm) Dedy D. Solihin Diah Nugrahani Pristihadi Dian Anggraini Djaswadi Dasuki Djoko Walujo Dody Dharmawan Trijuno, Dody Dharmawan Dondin Sajuthi Dwi Budiono Dwiranti, Astari Elpita Tarigan Eni Kusrini EVY AYU ARIDA Farid A. Moeloek Ferry Sandra Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Funahashi, Hiroaki Hadi, Restu S. Handina Rakhmawati Handina Rakhmawati Harry Murti Harry Murti Harry Murti Harry Murti Harry Murti Hartanti Kusumaningtyas HERA MAHESHWARI HERDIS Herdis . herdis herdis Heri Sujoko Heru Setijanto I Ketut Mudite Adnyana I Ketut Suatha I Wayan Batan Ichsan Ichsan Iis Diatin Iman Supriatna Indra Bachtiar Indra Kusuma Irma H Suparto Irma Herawati Suparto Irma Suryani ITA DJUWITA Ita Djuwita Ita Djuwita Ita Fauzia Hanoum, Ita Fauzia Ivan Sini Karisma Mardatillah Karisma Mardatillah KARTINI ERIANI Kartini Eriani KARTINI ERIANI Kartiwa, R. Angga Kelvin Yaprianto Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Krido Brahmo Putro Kusdiantoro Mohamad Kusumaningtyas, Hartanti Latifah Kosim Darusman Lea Tarliyah Lindiawati, Riris Lubis, Andri Maruli Tua M Agus Setiadi M. Haviz Madihah Madihah Madihah Madihah, Madihah Maman Surachman Mas Rizky A.A. Syamsunarno Maula, Yogi Nikmatul Miraprahesti, Retti N. Mohamad Fakhrudin Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Gunawan Muhammad Gunawan Muhammad Gunawan Muhammad Rizal Muhammad Rizal MUHAMMAD RIZAL MUHAMMAD RIZAL' Muhammad Rosyid Ridlo Muhammad Zairin JR Muhammad Zairin Jr. MULYOTO PANGESTU MULYOTO PANGESTU Muslim Muslim NASTITI KUSUMORINI Nastiti Kusumorini Nastiti Kusumorini Nining Handayani Nining Handayani Nining Handhayani Noer Muhammad Dliyaul Haq Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Nurhayati, Retno Wahyu Nurhidayat - Nurhidayat Nurhidayat Nurhidayat Nurhidayat Nuril Farizah Nursanti, Risa Nuzulia, Nur Aisyah Prakoso, Nurul Muhammad Prasetyaningtyas, Wahono Puspitasari, Riris Rachmat Herman Rahmaniyah, Wiwit Ridhani Rahminiwati, Min Rakhmawati, Handina Ramadhan Sumarmin Rangga Setiawan Rangga Setiawan Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Resti Rahma Dianti Ridi Arif Rimayanti - Rini Widyastuti Riris L. Puspitasari Riris L. Puspitasari Ronny Rachman Noor Salsabila, Cyntia Bella Sandy Qlintang SATRIYAS ILYAS Satya Gunawan Shofwal Widad Sigit Prastowo Siti Darodjah Rasad Situmeang, Adrian Sony H. Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO SONY HERU SUMARSONO Sri Catur Setyawatiningsih Srihadi Agungpriyono Subangkit, Mawar Sulistiono, Sumarsono, Sony H. Sumarsono, Sony Heru Sundari, Ayu Mulia Supar - Supar . Sutarya Enus Sutiman Bambang Sumitro TAKDIR SAILI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Jamaan Thomas Mata Hine Trevino A. Pakasi Tri Aprilliana Wulandari TRINIL SUSILOWATI Tutik Wrediati Tutik Wresdiyati Tutty Laswardi Yusuf Tutty Laswardi Yusuf Tuty Laswardi Yusuf Uswatun Hasanah Vincentia Maria Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin Wasmen Manalu Watanabe, Seiichi Widjiati w Wildan Mubarok Wining Astini Wining Astini Wiwit Ridhani Rahmaniyah Wulandari, Tri Aprilliana Yessie Widya Sari Yoga Yuniadi Yuhara Sukra Yuhara Sukra Yulnawati . YULNAWATI YULNAWATI Yundari, Yundari Yushinta Fujaya