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All Journal E-Journal of Linguistics E-Journal of Tourism Linguistika Jurnal Penelitian dan Pengembangan Pendidikan RETORIKA: Jurnal Ilmu Bahasa BERKALA SAINSTEK Jurnal Sains dan Teknologi Journal of Indonesian Tourism and Development Studies Jurnal Master Pariwisata (JUMPA) Biology, Medicine, & Natural Product Chemistry Ranah: Jurnal Kajian Bahasa BIOEDUSCIENCE Jurnal Biologi Tropis International Journal of Language and Literature Journal of Education Action Research Journal of Language and Literature Udayana Journal of Social Sciences and Humanities (UJoSSH) ABSYARA: Jurnal Pengabdian Pada Masyarakat Bioscientist : Jurnal Ilmiah Biologi International Journal of English and Applied Linguistics (IJEAL) International Journal of Social Science Berajah Journal Bahasa dan Seni: Jurnal Bahasa, Sastra, Seni, dan Pengajarannya International Journal of English Learning and Applied Linguistics (IJELAL) Gema Wisata Media Bina Ilmiah Journal of Scientific Research, Education, and Technology JURNAL BIOLOGI PAPUA Equator Science Journal (ESJ) Litera : Jurnal Bahasa dan Sastra Jurnal Ragam Pengabdian Journal of Biology Science and Education Jurnal Ekonomi, Manajemen Pariwisata dan Perhotelan Aksara
I Made Budiarsa
Program Studi Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas Tadulako, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract Windarsih, Yulia; Budiarsa, I Made; Trianto, Manap; Akram, Akram; Ashari, Abdul
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.831-841

Abstract

The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency Budiarsa, I Made; Trianto, Manap; Adnan, Bahana Aditya
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
Analisis Filogenetik Cryptic species Apis cerana Fabricius, 1793 Berdasarkan Gen 16S rRNA Puspitasari, Amira; Budiarsa, I Made; Ashari, Abdul; Dhafir, Fatmah; Windarsih, Yulia; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14967

Abstract

Apis cerana is a member of the Insecta class grouped in the order Hymenoptera. Phylogenetic analysis is used to describe the kinship of living things through phylogeny trees. This study aims to describe the phylogenetic relationship of cryptic species in Apis cerana based on the 16S rRNA gene. Sampling use the cruising method in various regions, the DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The result showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationship. A. cerana from Central Sulawesi is more closely related to other A. cerana species in the same clade, while other species are separated. The result of this study show a clear pattern of kinship between A. cerana populations.
Analisis Filogenetik Lebah Madu Endemik Sulawesi Apis nigrocincta Smith, 1860 Berdasarkan Gen 16S rRNA Hidyanisa, Dhea; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Nurdin, Musdalifah; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14968

Abstract

Apis nigrocincta is an endemic honey bee that is only found in two regions in Indonesia, namely the Sangihe Islands and Sulawesi Islands. Phylogenetic analysis is used to describe the phylogenetic relationship of Sulawesi endemic honey bee A. nigrocincta based on the 16S rRNA gene. Sampling using the cruising method in various regions, then DNA isolation, DNA amplification, sequencing, and continued bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationships. A. nigrocincta from Central Sulawesi is more closely related to other A. nigrocincta species in the same clade, while other species are separated. The results of this study show a clear pattern of kinship between A. nigrocincta populations. 
Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1) Rahayu, Irma Septi; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Masrianih, Masrianih; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14969

Abstract

Xylocopa appendiculata  is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Identifikasi Bakteri Escherichia coli dan Salmonella sp. pada Produk Ayam Geprek di Kantin Universitas Tadulako Rofiqoh, Rofiqoh; Kundera, I Nengah; Windarsih, Yulia; Buntu, Amalia; Budiarsa, I Made; Ashari, Abdul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15735

Abstract

This study aims to identify the presence of Escherichia coli and Salmonella sp. in "ayam geprek" products sold at the canteens of Tadulako University. The type of research used is descriptive-exploratory with quantitative examination methods using the Most Probable Number (MPN), Total Plate Count (TPC), and further identification using the Microbact System and Microbact System 2000 Software. Samples were taken from three different faculty canteens using a stratified random sampling technique. The results showed that (1) Test using the Most Probable Number (MPN) method obtained the highest value of 150x102 CFU / ml came from the FMIPA canteen sample, 28x102 CFU / ml samples from the FKIP canteen and the lowest value of 27x102 CFU / ml samples from the FKIP canteen. (2) Test using the Total PlateCount (TPC) method obtained the highest value of 105x103 CFU / ml came from the FMIPA canteen sample, 217x102 CFU / ml FKIP canteen sample, and the lowest value of 120x102 CFU / ml FKIP canteen sample. (3) Validation identification of the percentage of samples showing contamination by Escherichia coli and Salmonella sp., with a validation level above 90%. Thus, all samples in this study were positive for the presence of Escherichia coli and Salmonella sp. bacteria.
Pengaruh Ekstrak Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kadar Kolesterol Pada Tikus Putih (Rattus norvegicus) Yang Diinduksi LDL Zulfikar, Zulfikar; Ramadhan, Achmad; Mardin, Sutrisnawati; Isnainar, Isnainar; Budiarsa, I Made; Masrianih, Masrianih
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16020

Abstract

This study aims to determine the effect of Roselle flower extract (Hibiscus sabdariffa L.) on cholesterol levels in White Rats (Rattus norvegicus) induced by LDL. The research method used is a Laboratory experimental method with a Completely Randomized Design (CRD) pattern. The number of samples used was 24 White Rats consisting of 6 treatment groups and 4 replications. The normal control group (KN) is a group that is only given food and drink, the negative control group (K-) is given LDL induction for 30 days, the positive control group (K+) is given LDL induction for 30 days and simvastatin, groups PI, PII and PIII are given LDL induction for 30 days and Roselle flower extract doses of 50 mg/kg BW, 100 mg/kg BW and 150 mg/kg BW for 21 days. The data obtained were tabulated and analyzed by the one-way ANOVA statistical test using SPSS version-30. The results showed that Roselle flower extract positively contains bioactive compounds such as flavonoids and tannins and has a significant effect (p<0.05) on reducing cholesterol levels in White Rats induced by LDL. The effective dose to reduce cholesterol levels is a dose of 100 mg/kg BW and 150 mg/kg BW.
Identifikasi Jenis Bakteri Pada Layar Handphone Mahasiswa Pendidikan Biologi dan Pemanfaatannya Sebagai Media Pembelajaran Nabila, Putri; Kundera, I Nengah; Windarsih, Yulia; Sutrisnawati, Sutrisnawati; Budiarsa, I Made; Ashari, Abdul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16094

Abstract

This research aimed to isolate and identify the types of bacteria present on the surface of biology education students' mobile phone screens. This was an exploratory study using a bacterial isolation method.Bacteria found on the mobile phone screens were cultivated using MacConkey agar, a selective medium specifically designed to detect and identify Gram-negative bacteria, particularly Enterobacteriaceae like Escherichia coli. The accuracy of the Escherichia coli data from the phone screen samples, after processing with the Microbact System, was 96.39%.Malt Extract Agar (MEA) was also used as a selective medium to detect and identify Gram-positive fungi, such as Candida albicans.Based on these findings, the developed module was deemed highly feasible. A learning medium is considered highly feasible for use if it achieves a presentation rate between 60-100%. With an average score of 82.39%, the module meets this criterion and can be effectively used in learning.
Hubungan Kekerabatan Kepiting Biola (Uca spp.) di Desa Lalombi Kecamatan Banawa Selatan Kabupaten Donggala Berdasarkan Karakter Morfologi Biung, Yelsan Kanan; Budiarsa, I Made; Trianto, Manap; Kundera, I Nengah; Ashari, Abdul; Jayanti, Zulaikhah Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16380

Abstract

This study aims to analyze the phylogenetic relationships among six species of fiddler crabs (Uca spp.) found in Lalombi Village, Banawa Selatan Subdistrict, Donggala Regency, based on morphological characters. A descriptive-exploratory approach was employed, with purposive sampling to select representative specimens. Eighteen morphometric characters were measured from three individuals of each species: U. triangularis, U. perplexa, U. dussumieri, U. annulipes, U. tetragonon, and U. bellator. Data were analyzed using Principal Component Analysis (PCA) and UPGMA clustering with the aid of PAST software version 4.03. The PCA results revealed that carapace length (CL), anterior carapace width (ACW), and posterior carapace width (PCW) contributed significantly to total morphological variation. Cluster analysis indicated a close phylogenetic relationship between U. tetragonon and U. triangularis, as well as between U. perplexa and U. bellator. U. annulipes appeared intermediate between the two groups, suggesting a moderate level of relatedness. In contrast, U. dussumieri formed a distinct branch, likely due to its markedly larger body size compared to the other species. These findings affirm that morphometric characters are valuable in elucidating phylogenetic relationships within the genus Uca and support taxonomy based on morphological traits.
Pengaruh Ekstrak Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kadar Glukosa Darah Pada Tikus Putih (Rattus norvegicus L.) Yang Diinduksi Aloksan Faradila, Ratna; Ramadhan, Achmad; Sutrisnawati, Sutrisnawati; Isnainar, Isnainar; Dhafir, Fatmah; Budiarsa, I Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16643

Abstract

This study aims to determine the effect of rosella flower extract on reducing blood glucose levels in alloxan-induced white rats. The research method used is an experiment conducted in the laboratory with a complete randomized design (RAL) pattern. The number of samples used was 24 white rats consisting of 6 treatment groups and 4 replicates. Normal control group (Kn) is a group that is only given food and drink, negative control group      (K-) is a group given alloxan induction, positive control group (K+) is a group given alloxan induction and metformin synthesized drug, treatment groups I, II, and III are groups given alloxan induction and rosella flower extract at a dose of 50 mg/kg BB, 100 mg/kg BB and 150 mg/kg BB. The data obtained were analyzed by ANOVA statistical test. The results showed that rosella flower extract had an effect on reducing blood glucose levels in white rats induced by alloxan. The most effective dose in reducing blood glucose levels in white rats is a dose of 150 mg/kg.
Co-Authors ., Reskiana Abd Hakim Laenggeng, Abd Hakim Abd Rauf Achmad Ramadhan Achmad, Mestawaty As. Ahmad Ramadhan Akram, Akram Amalia Buntu Amiruddin Kasim Anak Agung Putu Agung Suryawan Wiranatha Anak Agung Putu Putra Aro Meko Mbete Aron Meko Mbete Arya Wahyu Widiantara, I Putu Ashari, Abdul Astawa, I Gede Astija Avita, Cindy Dea Bahana Aditya Adnan Bialangi, Mursito S. Biung, Yelsan Kanan Burhanuddin, Alga Patricia Chatarina Umbul Wahyuni Denok Lestari Dewa Ayu Widiasri Dewi Tureni Dwi Lina Sari Tanjung Elza, Tiara Faradila, Ratna Fardini Sabilah Fatmah Dhafir Febri Aspitasari Gede Primahadi Wijaya Rajeg Hasan, Isharyadi Hasanudin, Rezki Hayyatun Mawaddah Herry Arianto Hidyanisa, Dhea Hj. Musdalifah Nurdin I Gde Pitana, I Gde I Gede Putra Nugraha I Gusti Ayu Gede Sosiowati I Gusti Ayu Krisnawati I Ketut Artawa I Ketut Murdana I Ketut Purwata I Ketut Riana I Ketut Wardana I Made Antara I Made Darsana I Made Netra I Made Rai Jaya Widanta I MADE RAJEG I Made Suastra I Nengah Kundera I Nengah Laba I Nengah Sudipa I Nyoman Sudiarta I Nyoman Sunarta I NYOMAN SUPARWA I Nyoman Udayana I WAYAN PASTIKA I Wayan Simpen I Wayan Sugita Ida Ayu Made Puspani Ida Bagus Putra Yadnya Ilahi, Wahyu Abdul Isninar, Isnainar Jesy Valenia Panggabean Kadek Feni Aryati Kadek Purnata Kusumaningati, Lilis Tri Latompai, Nismawati I. Lestari M.P Alibasyah Lilies Lilies Made Antara Made Antara Made Sri Satyawati Maharani, Mirfadz Manap Trianto Manap Trianto MARIA BINTANG Marisa, Fajri Masriani . Messe, Yunita Mohammad Jamhari Muchlis L. Djirimu Muh Annas Abdillah Muhammad F. Rahman Mursito S. Bialangi Nabila, Putri Nasution, Annio Indah Lestari Nasution, Rahmad Kurnia Abdik Ni Ketut Sri Rahayuni Ni Luh Nyoman Seri Malini Ni Luh Sutjiati Beratha Ni Luh Yuniarti Ni Made Dhanawaty Ni Made Suryati Ni Putu Herni Susanti Ni Wayan Sartini Novita Mulyana NOVITASARI Nur Alam Nurwahda, Nurwahda Nyoman Ariana Nyoman Surya Wijaya Patigu, Rosi Feraningsih Permatasari, Ni Kadek Dewi Prabhaswari, Putu Indry Puspitasari, Amira Putu Dewi Merlyna Yuda Pramesti Putu Prasanthi Vahini Putu Sandra Putri Astariani Rachmad Suwardi Rafiqa RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi rofiqoh rofiqoh Safitri, Gusti Ayu Kadek Yunita Salsabila Husna Ramadhani Amara Samsurizal, M. Sulaeman Saridewi, Uut Shamdas, Hj. Gamar B.N SULFIANTI, SULFIANTI Suprianto Suprianto Suprianto Susanti, Putu Herny Sutrisnawati Mardin Syamsul Alam Paturusi Syech Idrus Syech Zainal Tandi, Jenifa Arisa Thelisa . Tulak, David Pebriyanto Tuna, Anjeli Wayan Darma Sedana Widiastuti . Windarsih, Yulia Yafed Syufi Yafet Syufi Yuni Masyita Dewi Zulaikhah Dwi Jayanti