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All Journal ACTA VETERINARIA INDONESIANA Media Kedokteran Hewan Briliant: Jurnal Riset dan Konseptual Caraka Tani: Journal of Sustainable Agriculture Jurnal Medik Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Ilmu Peternakan Terapan Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Bantara Journal of Animal Science Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Innovation Studies Journal of Fisheries & Marine Jurnal Agro Veteriner Folia Medica Indonesiana
Al arif, Mohammad Anam
Departemen Peternakan, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C UNAIR Mulyorejo, Surabaya, Jawa Timur, Indonesia 60115
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Journal : Journal of Fisheries

 1 
Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.
Co-Authors Agung Prasetyo Agustono, Bodhi Aksono HP., Eduardus Bimo Alfanindya, Evita Aisyah Ali Agus Amal, Mohammad Noor Azmai Amung Logam Saputro Anastasya, Jelita Andreas Berny Yulianto, Andreas Berny Andriani, Asafarid Annas Salleh Annisa Nur Aulia Purwantoro Ardianto Ardianto Aris Puji Nugroho Aryaloka, Suhita Ayuti, Siti Rani Balgis Al Basyarahil Bambang Prajogo E.W Cantika Putri Melyana Caska - Chandra, Evania Haris Chusniati, Sri Dadik Rahardjo Dadik Rahardjo, Dadik Daruti Dinda Nindarwi Devi Sri Rohmani Dewi, Nina Nurmalia Dian Ayu Permatasari Dimas Kunto Satrio Dita Wisudyawati Djoko Legowo Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri ERMA SAFITRI Faiqoh, Berliana Elok Faisal Fikri Fatin Fadhilah Hasib Fauzia, Kartika Afrida Fauziah, Ima Fuddin, Muhammad Nazar Gandul Atik Yuliani Gazali Salim Gesa Cantya Primatara Hadiah Fitriyah Hani Plumeriastuti Hapsari, Tiara Harijani, Nenny Hasib, Abdullah Hendarti, Gracia Angelina HERAWATI, LILIK Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Hussain, Muhammad Asif IMAM MUSTOFA Iqbal Laksana Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Amelia Lintang Putri Koesnoto Soepranianondo Koesnoto Supranianondo Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasih, Dea Anita Ariani Kusala, Muhammad Khaliim Jati Laksana, Iqbal Lilik Maslachah Lovela, Aprinda Ratna Ma'ruf, Muchammad Achsinul Fikri Mafruchati, Maslichah Maghfiroh, Nurutin Tutur Bifatikhatil M Melyana, Cantika Putri Miarsono, Sigit Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Sofiul Anam Moh. Zaky ‘Ubaidillah Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Mohammad Sukmanadi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Agil Gumilang Muhammad Ilyas Wahyudi M’ Izi Kumala Lazuardi Sultoni Nabil Fariz Noorrahman Nove Hidajati Novianti, Arindita Niatazya Nusdianto Triakoso Poedji Hastutiek Pramestya, Ninda Rubi Prasinta, Redilla Prastiya, Ragil Angga Pudji Srianto Putri Desi Wulan Sari Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmaniar Larasati Rahmawati, Kadek Rahmi Sugihartuti Rara Irbah Fitriana Karimah Ratna Damayanti Rehman, Saifur Rejeki, Purwo Sri Riza Zainuddin Ahmad Rizki, Andaru Rosyada, Zulfi Nur Amrina Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Santanumurti, Muhammad Browijoyo Saraswati, Adyayutti Wijang Sari, Putri Desi Wulan Sarmanu, Sarmanu Sheila Marty Yanestria Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soepranianondo, Koesnoto Sony Wibisono Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Pantja Madyawati Suharsono Suharsono Suherni Susilowati Sukmawati Lailatul Jannah Supriyadi Supriyadi Suwarno Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Titik Yuliarini Trilas Sardjito Tyasningsih, Wiwiek Utomo, Gogik Satrio Margo Wardhana, Dhandy Koesoemo Wardhani, Bantari Wisynu Kusuma Wardhani, Indrayuni Lukitra Wardiana, Nurul Ika Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibowo, Syahputra Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Wurlina, W Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zulfanisa, Rizky Asrin