The research was conducted in the greenhouse of the Faculty of Agriculture, Universitas Islam Sumatera Utara. The aim of this research is to know the effect of chicken manure and N fertilizer on growth and production of mustard pakcoy plant with parallon vertikultur system. The design of this study used a Completely Randomized Design (CRD) Factorial consisting of two factors and repeated three times. The first factor is the provision of chicken coop (A) which consists of 4 levels, namely: A0 = 0 g / parallon (Control); A1 = 1000 g / parallon; A2 = 2000 g / parallon; A3 = 3000 g / parallon. The second factor is N (N) fertilizer consisting of 4 levels, namely: N0 = 0 g / parallon (Control); N1 = 1.8g / plant; N2 = 3.7g / plant; N3 = 5.6g / plant. The results showed that chicken manure gave a very significant effect on all parameters observed. The provision of chicken manure has a very significant effect on the parameters of plant height, the number of leaves, the diameter of the cob. The results showed that N fertilizer gave very significant effect on the parameters of plant height, leaf number, diameter of cob. The interaction of chicken manure and N fertilizer gave a very real effect on the diameter of the hump. However, the effect is not significant on the parameters of plant height, number of leaves.