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The Quality of Kampung Broiler (KB) Chicken Frozen Semen with DMA Concentrations on Yolk Lactate Ringer Diluent J. Junaedi; Raden Iis Arifiantini; Cece Sumantri
Chalaza Journal of Animal Husbandry Vol 2, No 2 (2017): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (819.231 KB) | DOI: 10.31327/chalaza.v2i2.292

Abstract

Chicken Kampung Broiler (KB) is the result of a cross between a local chicken (chicken) with broiler imported chicken (broiler chicken). Testing the quality of frozen chicken semen from crossing needs to be done to get high productivity. This study aims to obtain a concentration of cryoprotectant Dimethyl Acetamide (DMA) the best of the concentration of 5%, 7% and 9% in chicken semen freezing KB. The semen used in this study came from three chickens of KB and repeated as many as nine times. Semen is collected by the method of message three times a week. Semen is evaluated macroscopically and microscopically. Subsequently spermatozoa were diluted with yolk diluent and the addition of three DMA cryoprotectant concentrations (5%, 7%, and 9%). Semen diluted 0:25 ml is packed into straw. Then equilibrated at a temperature of 5 ° C for two hours.  After equilibration evaluation of motility and viability of spermatozoa. It is then frozen on liquid nitrogen vapor for 10 minutes. Frozen semen is then stored in liquid nitrogen containers at a temperature of -196 ° C. After 24 hours, the thawing semen in 37 ° C for 30 seconds. The results showed that the addition of DMA 9% in Ringer's lactate diluent yolk produces a percentage motility and viability after thawing is greater than 5% and DMA 7%. Neither the addition of DMA 9% concentration can increase recovery rate after thawing
Association of DGAT1 Gene Related to Flavor, Odor, Cholesterol, and Mineral in Indonesian Sheep Fisma Amri; Ratna Sholatia Harahap; Cece Sumantri; Ismeth Inounu; Depison Depison; Yun Alwi; Asep Gunawan
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 2 (2023): June 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i2.3152

Abstract

Diacylglycerol acyltransferase 1 (DGAT1) is a potential candidate gene for improving Indonesian lamb’s quality and nutrient value.  The study aimed to identify the diversity of the DGAT1 gene with SNP g.8539 CT and its relation to Indonesian lamb's flavor and odor, cholesterol, and mineral.  Total of 254 ten to twelve months old sheep consisted of 20 Javanese fat-tail sheep (JFTS), 107 Javanese thin-tail sheep (JTTS), 10 Garut composite sheep (GCS), 10 Compass agrinak sheep (CAS), 10 Barbados cross sheep (BCS), 20 Garut sheep (GS), 27 Jonggol sheep (JS), and 50 Jambi local sheep (JLS).  One hundred sheep were used to analyze the association of the DGAT1 gene with flavor and odor content, cholesterol, and mineral.  The diversity of DGAT1|AluI was analyzed with the PCR-RFLP method.  The association of the DGAT1 gene with the nutritional value of meat was analyzed using the GLM (General Linear Model) method.  The results showed that the DGAT gene was polymorphic in JTTS, GS, and JFTS and monomorphic in CAS, BCS, JLS, and GCS.  CC and CT genotypes were found in JTTS, GS, and JFTS.  SNP g.8539 CT of DGAT1 gene had a significant association (P0.05) with flavor and odor, 4-Ethyloctanoic Acid (EOA).  However, the DGAT1 gene had no significant association (P0.05) with cholesterol and mineral.  The DGAT1 gene might be marker-assisted selection for improving lamb flavor and odor in Indonesian sheep.
Characterization and Association of CFHR5 Gene Polymorphism with Fatty Acid Composition and Cholesterol in Sheep Akbanugra Yudhananda; Kasita Listyarini; Cece Sumantri; Asep Gunawan
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.84177

Abstract

The Complement Factor H Related 5 (CFHR5) gene is speculated to have an important role in regulating fatty acid composition in sheep. The aim of this study was to investigate the polymorphism of the CFHR5 gene and its association with fatty acid composition and cholesterol of sheep in Indonesia. A total of 172 rams from 83 priangan sheep (PS), 20 sapudi sheep (SS), 19 Garut sheep (GS), 20 jonggol sheep (JS), 10 Garut composite sheep (GCS), 10 compass agrinac sheep (CAS), and 10 Barbados cross sheep (BCS) were used for this study. Identification of CFHR5 gene polymorphism were performed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using AciI restriction enzyme. The results showed that the CFHR5 gene in all sheep populations were polymorphic producing three genotypes, e.g. CC, CT and TT, respectively. The polymorphism had a significant effect (p<0.05) on fatty acid composition (miristoleic [C14:1], ginkgolic [C17:1], tridecanoic [C13:0], and heptadecanoic [C17:0] acids) and cholesterol. The breed and polymorphism had a significant effect (p<0.05) on fatty acid composition (JS with tridecanoic acid [C13:0] and BCS with miristoleic acid [C14:1]). The CC genotype is the preferred genotype and as it exhibits reduced levels of saturated fatty acids and cholesterol. The BCS sheep is the preferred genotype and as it exhibits high PUFA/SFA ratio. The CFHR5 gene (SNP c.1011C>T) has the potential to be used as a genetic marker for the selection of low saturated fatty acid composition and cholesterol in sheep
Quality of IPB-D1 Chicken Egg Yolk Powder Using Various Drying Methods Salsabila Ma’shum Imawan; Zakiah Wulandari; Tuti Suryati; Cece Sumantri
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 18 No. 3 (2023)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2023.018.03.2

Abstract

IPB D-1 chickens had good egg productivity. Chicken eggs are often used as functional ingredients in the form of powder because they have many advantages for the food industry. This research aims to produce egg yolk powder with good physicochemical, microbiological, and fatty acid qualities derived from IPB-D1 chicken eggs using various drying methods namely spray drying, oven drying, and vacuum drying. This study also aims to examine the functional properties of IPB-D1 chicken egg yolk powder by application to mayonnaise. It is compared with mayonnaise made from fresh egg yolks and commercial egg yolk powder. The experimental design used in this research was a randomized block design (RBD) based on the manufacturing period. The result showed that different drying methods for egg yolk powder can affect the emulsion activity, total plate count (TPC), aw, pH, water, ash, and fat content. The drying method did not affect the protein content and emulsion stability. Egg yolk powder with oven drying gave the best results for quality.
Assessment of Commercial DNA Extraction Kits for Porcine Gelatin Detection Using RT-PCR and ddPCR Gina, Safira; Rahmagiarti, Cintera; Ummah, Isna Mustafiatul; Sumantri, Cece; Suparto, Irma Herawati; Darmawan, Noviyan
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.605-612

Abstract

Gelatin is extensively applied in various industries, including food, beverages, cosmetics, and pharmaceuticals. Although the determination of gelatin species is essential for religious, health, and consumer preference reasons, a standardized analysis method is absent. The challenge in identifying gelatin through DNA-based methods arises from the low DNA content and extensive DNA denaturation in the gelatin matrix. This study assessed the efficacy of two commercial DNA extraction kits, namely the Processed Food DNA Extraction (PF kit) and the DNeasy Mericon Food Kit (DM kit), for extracting DNA from porcine gelatin powder and commercial products derived from gelatin. Additionally, we evaluate the amplification of the extracted porcine DNA using real time polymerase chain reaction (RT-PCR) and droplet digital polymerase chain reaction (ddPCR) techniques. The PF extraction kit demonstrated successful DNA extraction from porcine gelatin powder and commercial samples of porcine gelatin-based candies with a higher concentration (32.24-286.07 ng/uL) and purity (A260/A280 ratio of 1.82-2.33) compared to the DM kit (3.95-7.30 ng/uL and an A260/A280 ratio of 1.29-2.45). RT-PCR and ddPCR analyses yielded positive results for porcine DNA from gelatin powder for both PF and DM kits, albeit with differing Cq values and copy numbers. The choice of DNA extraction kit significantly impacted the amplification results when analyzing commercial samples of porcine gelatin-based candies. Using RT-PCR, all samples yielded negative results with the DM kit, while the PF kit detected one positive result for porcine DNA. Improved outcomes were observed with more sensitive analysis methods such as ddPCR, where the DM kit identified one positive result for porcine DNA while the PF kit detected positive results for all tested candies.
Evaluasi Keragaman Gen Selenoprotein W1 (SEPW1) dan Kaitannya Terhadap Komposisi Asam Lemak Pada Domba Yusuf, Salman; Harahap, Ratna Sholatia; Listyarini, Kasita; Rahayu, Sri; Sumantri, Cece; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.109 KB) | DOI: 10.33772/jitro.v9i1.17717

Abstract

Kandungan asam lemak dipengaruhi oleh sejumlah gen dan di antaranya ialah selenoprotein W1 (SEPW1). Eksperimen ini bermaksud untuk mengetahui polimorfisme gen SEPW1 serta hubungannya terhadap kandungan asam lemak di domba. Sejumlah 102 ekor domba yang tersusun dari 20 domba ekor gemuk (DEG), 30 domba ekor tipis (DET), 9 domba komposit garut (DKG), 10 domba compass agrinak (DCA), 10 domba barbados cross (DBC), dan 23 domba jonggol (DJ) digunakan dalam penelitian ini. Analisis polimorfisme gen SEPW1 dicari dengan memanfaatkan Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP), enzim BanI, serta produk amplifikasi berukuran 478 bp. Analisis Chi-square memberikan hasil bahwa populasi berada dalam keseimbangan Hardy-Weinberg, kecuali sampel domba jonggol. Polimorfisme gen SEPW1 teramati berada di semua sampel rumpun domba. Single nucleotide polymorphism (SNP) g.53617063 G>A gen SEPW1 berasosiasi (P<0,05) dengan asam laurat (C12:0), asam palmitat (C16:0), asam arakidat (C20:0), asam henekosilat (C21:0), asam miristoleinat (C14:1), asam oleat (C18:1n9c), asam linoelat (C18:2n6c) dan asam lemak total. Genotipe GG memiliki kandungan asam lemak tak jenuh yang paling tinggi dibandingkan genotipe lainnya. Gen SEPW1 memiliki kapasitas untuk dikembangkan sebagai penanda asam lemak di domba.
Selenoprotein W1 (SEPW1) Gene Polymorphism and Association with Mineral Content in Sheep Astira, Adinda Wanda; Harahap, Ratna Sholatia; Sumantri, Cece; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 10, No 1 (2023): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v10i1.27066

Abstract

Selenoprotein W1 (SEPW1) is a protein containing selenium that has an important role in muscle growth and development by protecting myoblasts from oxidative stress. This study was aim to identify the diversity of the SEPW1 gene and its association with mineral content in sheep. The samples used were 100 sheep consisting of 10 samples of compass agrinak sheep (CAS), 15 samples of Javanese fat-tailed sheep (JFT), 30 samples of Javanese thin-tailed sheep (JTT), 25 samples of garut composite sheep (GCS), and 20 samples of jonggol sheep (JS). Selected mineral contents were determined using the IK method LP-04.10-LT-1.0. Identification of SEPW1 gene polymorphism will be conducted using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The size of the amplification product was 455 bp. Chi-square analysis showed that the population was in Hardy-Weinberg equilibrium. Polymorphism of the SEPW1 gene was found in each breed of sheep. The SEPW1 gene’s SNP g.53617063 A>G was significantly (p
Determining the Matrilineal Origin of Indonesian Kerinci Duck Breed (Anas platyrhynchos) Based on MT-ND2 Gene Diversity Depison; Putra, Widya Pintaka Bayu; Gushairiyanto; Gunawan, Asep; Sumantri, Cece
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.581-588

Abstract

The Kerinci duck (Anas platyrhynchos), hailing from Jambi Province, represents one of the local duck breeds of Indonesia. This study aimed to ascertain the matrilineal origin of Kerinci ducks through analysis of the mitochondrial NADH Dehydrogenase subunit 2 (MT-ND2) gene. In this pursuit, forward sequences of the MT-ND2 gene (490 bp) were extracted from unsexed Kerinci ducks, totalling forty-eight (48) sequences, procured from blood samples. The findings demonstrated the identification of fifteen (15) distinct haplotypes within the MT-ND2 gene, with corresponding haplotype diversity (Hd) and nucleotide diversity (pi) values of 0.74 and 0.003, respectively. The resulting phylogenetic tree unveiled that Kerinci ducks exhibit two matrilineal origins: an Asian and an independent Kerinci lineage. Moreover, most Kerinci ducks were categorised within the H4 group (24 birds) of the Asian lineage. Nevertheless, this study also revealed the existence of an independent Kerinci lineage comprising eight (8) duck haplotypes. In conclusion, the analysis of the MT-ND2 gene underscored the genetic introgression of A. zonorhyncha and A. poecilorhyncha in Kerinci ducks.
Cattle weight prediction model using convolutional neural network and artificial neural network Yulianingsih, Yulianingsih; Nurdiati, Sri; Sukoco, Heru; Sumantri, Cece
Indonesian Journal of Electrical Engineering and Computer Science Vol 36, No 1: October 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v36.i1.pp441-449

Abstract

The weight of livestock is a crucial metric for evaluating management efficacy, informing policy decisions, and determining the market value of animals. In certain scenarios, conventional methods such as physical weighing and measurement calculations can prove challenging, including the absence of livestock health records or weighing equipment. This research aims to develop a predictive model for estimating the live weight of cattle through visual assessments and metadata, including age and pixel count, utilizing a combination of convolutional neural network (CNN) and artificial neural network (ANN) methodologies. A total of 223 data were obtained from a local farm before augmentation. The model's predictive capability was successfully demonstrated, with its performance quantified by an average mean absolute percentage error (MAPE) of 10% on test data. This study demonstrates that through the combination of CNN and ANN, as well as optimal parameter tuning, efficient prediction of cattle weight can be achieved.
Performa Pertumbuhan Ayam IPB-D1 pada Perlakuan Pakan dan Manajemen Pemeliharaan yang Berbeda Muhammad Fikri Al Habib; Sri Murtini; Luci Cyrilla; Irma Isnafia Arief; Rita Mutia; Cece Sumantri
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16375

Abstract

ABSTRAK. Pelepasan ayam IPB-D1 sebagai rumpun baru yang disebar di masyarakat maka sistem pemeliharaannya secara umum bersifat ekstensif dan pakan yang diberikan memanfaatkan pakan lokal. Tujuan penelitian ini adalah untuk mengetahui performa ayam IPB-D1 dengan perlakuan pemeliharaan P1 (sistem kandang Closed House dan penggunaan 100% pakan komersil) serta perlakuan P2 (sistem kandang umbaran dan pencampuran pakan lokal hingga 70%). Penelitian ini menggunakan ayam IPB-D1 berjumlah 503 ekor DOC yang dipelihara hingga umur 12 minggu. Performa ayam diukur berdasarkan bobot badan, laju pertumbuhan, konsumsi pakan, konversi pakan serta tingkat kematian ayam pada umur DOC-12 minggu. Data dianalisis secara deskriptif dengan menyajikan rataan, simpangan baku, koefisien keragaman dan analisis statistik menggunakan uji T. Hasil penelitian performa ayam IPB-D1 pada perlakuan P1 diperoleh bobot badan jantan 1378.2694.56 g dan betina 1178.33119.33 g, sedangkan pada perlakuan P2 diperoleh bobot badan jantan 1173.8260.1 g dan betina 957201.7 g. Laju pertumbuhan pada kedua perlakuan bersifat positif, serta konversi pakan pada perlakuan P1 (2.88) dan P2 (3.44) cukup baik. Kesimpulan penelitian ini adalah ayam IPB-D1 diperoleh performa yang sangat baik pada perlakuan P1 dan mampu beradaptasi pada perlakuan kandang sistem umbaran serta mampu memanfaatkan campuran pakan lokal yang baik pada perlakuan P2. (The growth performance of IPB-D1 chickens in different feed treatments and production systems) ABSTRACT. The release of the IPB-D1 chicken as a new breed that is spread in the community, the production system is generally extensive and the feed provided utilizes local feed. The purpose of this study was to determine the performance of IPB-D1 chicken with production treatment P1 (Closed House cage and use of 100% commercial feed) and P2 treatment (outdoor cage and use local feed mixing up to 70%). This study used 503 DOC IPB-D1 chickens which were maintained until 12 weeks of age. Chicken performance was measured based on body weight, growth rate, feed consumption, feed conversion and chicken mortality at 1-12 weeks. Data were statistically analyzed using the T test and descriptive analysis with means, standard deviations, and coefficient of diversity. The results of the study of the performance of IPB-D1 chickens in P1 treatment obtained male body weights 1378.26 94.56 g and females 1178.33 119.33 g, while in P2 treatment obtained male body weights 1173.8 260.1 g and females 957 201.7 g. Growth rates in both treatments were positive, and feed conversion was good in P1 treatments (2.88) and P2 treatments (3.44). The conclusion is that the IPB-D1 chicken obtained a very good performance in the P1 treatment and was able to adapt to the treatment of outdoor cages and was able to utilize the local feed mixture in the P2 treatment.
Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Amri, Fisma Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman TIKE SARTIKA Tike Sartika Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum