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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Ilmu Ternak Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Science and Technology Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Jurnal Aplikasi Teknologi Pangan Jurnal Ilmiah Fillia Cendekia Building of Informatics, Technology and Science ZIRAA'AH MAJALAH ILMIAH PERTANIAN Indonesian Journal of Electrical Engineering and Computer Science Jurnal Ilmiah Sains Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Animal Production Annales Bogorienses

Cece Sumantri

Department Of Animal Production And Technology, Faculty Of Animal Science, IPB University, Bogor, 16680, Indonesia

Author-ID : 2751185

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Karakterisasi Plantarisin IIA-1A5 sebagai Antimikroba dan Evaluasi Aktivitas Sediaan Kering Beku Terenkapsulasi Mochammad Sriduresta Soenarno; Irma Isnafia Arief; Cece Sumantri; Epi Taufik; Lilis Nuraida
Jurnal Aplikasi Teknologi Pangan Vol 9, No 1 (2020): February 2020
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Bakteriosin adalah peptida dengan aktivitas antibakteri yang diproduksi oleh bakteri asam laktat dan digunakan sebagai pengawet alami. Penelitian sebelumnya menunjukkan bahwa Lactobacillus plantarum IIA-1A5 memproduksi bakteriosin yang diberi nama Plantarisin IIA-1A5 pada medium pertumbuhan yang dibuat dari whey yang diperkaya skim. Untuk aplikasi sebagai pengawet alami dan untuk memperbaiki masa simpan dan aktivitas anti mikrobanya, plantarisin perlu dienkapsulasi dan dikeringbekukan. Tujuan dari penelitian ini adalah untuk mengkarakterisasi dan mengevaluasi aktivitas antimikroba dari sediaan plantarisin IIA-1A5 yang terpurifikasi parsial dan terenkapsulasi kering beku. Ekstraksi dan purifikasi dari bakteriosin dimulai dengan presipitasi dengan ammonium sulfat, yang diikuti dengan dialysis, dan penukar kation kromatografi. Purifikasi parsial dari plantarisin kemudian dimikroenkapsulasi dengan maltodextrin kemudian dilanjutkan dengan proses kering beku. Berdasarkan pada SDS-PAGE, fraksi protein ke-7 (F7) dari plantarisin yang dipurifikasi parsial memiliki pita tunggal dan berat molekul sekitar 9,65 kDa. Konfirmasi lebih lanjut dengan menggunakan MALDI-TOF MS, ternyata pita tunggal tersebut terdiri dari 5 peptida yang diidentifikasi berbobot molekul masing-masing sebagai berikut 5,5, 7,80, 7,96, 9,09, dan 9,27 kDa. Plantarisin kering beku memiliki aktivitas antimikroba terhadap Staphylococcus aureus tiga kali lipat dibandingkan dengan aktivitas antimikroba dari supernatan bebas sel, dan lebih tinggi dibandingkan dengan nisin, namun kurang bila dibandingkan dengan antibiotik ampisilin dan penisilin. Kesimpulannya, aktivitas antimikroba plantarisin kering beku dapat ditentukan dan lebih tinggi dibandingkan dengan nisin, ampisilin dan penisilin.Characterization of Plantarisin IIA-1A5 as Antimicrobial subtances and Evaluation of Acitivity of Freeze-dried Microencapsulated PreparationAbstractBacteriocins are peptides with antibacterial activity produced by lactic acid bacteria and used as natural preservatives. Previous studies showed that Lactobacillus plantarum IIA-1A5 produces bacteriocin named plantaricin IIA-1A5 in the medium consisting whey enriched with skim milk. For application as food preservatives and to improve its shelf-lie and activity, plantaricin was needed to be microencapsulated and freeze dried. The objective of this research was to characterize and evaluate the activity of partially purified freeze dried microencapsulated plantaricin IIA-1A5. Characterisation of partially purified plantaricin IIA-IA5 includes the identification of active fractions and molecular weight, evaluation of activity at different stage of purification and evaluation of antimicrobial activity of freeze dried microencapsulated plantaricin IIA-IA5. Extraction and prificafication of the bacteriocins started with precipitacion with ammonium sulfate, followed by dialysis, and cation exchange chromatography. The partial purified of plantaricin was then microencapsulated in maltodextrin followed by freeze drying. Based on SDS-PAGE, the protein fraction F7 of partially purified plantaricin had a single band and molecular weight about 9.65 kDa. Further analyses using MALDI-TOF, it revealed that five peptides were identified from one single band plantaricin with molecular weight 5.5, 7.80, 7.96, 9.09, and 9.27 kDa, respectively. The freeze dried plantaricin freeze showed antimicrobial activity against Staphylococcus aureus three times stringer as compared to the activity of cell free supernatant, and was higher than nicin, but less than antibiotic ampicilin and penicilin. As concusion, the activity of freeze dried plantaricin could be determined and had a higher value than nicin, ampicilin and penicilin.

Produksi Embrio In Vivo di Balai Embrio Ternak (BET) Cipelang dan Tren Perkembangan Embrio Transfer Global Irma Irma; Siti Darodjah; Nena Hilmia; Oloan Parlindungan; Ani Rosmayanti; Sri Wahyuni Siswanti; Septaria Jodiansyah; Darlian Fachruddin; Laelatul Choiriyah; Sikin Sikin; Weni Kurniati; Cece Sumantri
Jurnal Ilmiah Fillia Cendekia Vol 7 No 1 (2022): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v7i1.2338

The development of the global genetic market has change in strategy, large-scale genetic companies, and artificial insemination has driven the industrialization of embryo market. As a result, the demand for embryo production is increasing. The purpose of this report is to briefly explain the current situation of the in vivo embryo production at the Livestock Embryo Centre (LEC), and review the development trends of global embryo transfer. In vivo embryo production data comes from secondary data for 2014-2020 from the LEC, while world transfer embryo development data comes from embryo transfer association. The average of ova (oocyte and embryo) ranged from 7-10 ova/flushing (averages 8 ova) with transferable embryos ranging from 3-5 embryos/flushing (average 4 embryo). Percentage of transferable embryo ranged from 36%-54% (averages 48%). Estimated numbers of ova and transferable embryos per flush in the world were 10.5 and 6.4 in 2019, respectively. In general, the average production of in vivo embryos and transferable embryos at LEC increases every year. Trend of world embryo production increases while in vivo embryo decreased accompanied by an increase in in vitro embryo production.

Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Cece Sumantri; Nurhayati D; Ahmad Farajallah; A Anggraeni
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Anima Science, Bogor Agricultural University were studied. Research activities were carried out through some step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P R) then genotyped by single strand conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on the exon 7. The electrophoresis pattern revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%) respectively. There were no significant effect polymorphism β-lactoglobulin gene on both milk yield and quality in I cal sheep at JASTRU. Keywords : polymorphism. β-lactoglobulin in gene, PCR-SSCP and local sheep

IDENTIFIKASI SIFAT KUALITATIF DAN KUANTITATIF SAPI RANCAH Nena Hilmia; Ronny Rahman Noor; Cece Sumantri; Dedi Rahmat; Rudi Priyanto
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 44, No 3 (2019)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v44i3.2227

Karakterisasi sifat kualitatif dan kuantitatif diperlukan untuk menggali potensi sapi local sebagai informasi dasar untuk mengembangkan sumber daya genetic sapi tersebut. Penelitian bertujuan untuk mengidentifikasi sifat kualitatif dan kuantitatif sapi Rancah di Kabupaten Ciamis. Sampel yang digunakan adalah 92 ekor sapi Rancah betina, umur > 3 tahun, masing masing 40 ekor dari kecamatan Tambaksari dan 52 ekor dari kecamatan Cijulang. Parameter yang diukur adalah variasi warna bulu, dan ukuran-ukuran tubuh pada kedua daerah penelitian. Data dianalisis secara deskriptif, perbedaan parameter bobot badan dan ukuran tubuh pada kedua populasi penelitian dianalisis dengan Uji T. Hasil penelitian menunjukkan Warna bulu dominan sapi Rancah adalah merah bata. Bobot badan dan ukuran tubuh di Cijulang lebih besar dibandingkan dengan Tambaksari, kecuali panjang dan lebar kepala sama. Bobot badan dan ukuran-ukran tubuh sapi Rancah berada diantara sapi Bali dan PO.

JARAK GENETIK POPULASI KUDA LOKAL SULAWESI UTARA BERDASARKAN ANALISIS MORFOLOGI DAN POLIMORFISME PROTEIN DARAH Ben J. Takaendengan; Ronny R. Noor; Cece Sumantri; Sri Adiani
JURNAL ILMIAH SAINS Volume 11 Nomor 1, April 2011
Publisher : Sam Ratulangi University

Penelitian ini bertujuan untuk menentukan jarak genetik antar subpopulasi kuda lokal di Sulawesi Utara dengan menggunakan analisis morfologi dan polimorfisme protein darah. Subpopulasi kuda local yang diamati meliputi 505 ekor kuda dewasa yang terdiri dari 310 kuda jantan (stallion) dan 193 kuda betina (mare) dengan umur 2-7 tahun. Analisis morfologi digunakan untuk menentukan ukuran tubuh dan bobot badan, sedangkan analisis polimorfisme protein darah dilakukan untuk menduga keragaman genetic berdasarkan polimorfisme protein Albumin (Alb), post-albumin (PAlb), transferin (Tf), post-transferin 1 (PTf-1), post-transferin 2 (PTf-2), alfa-hemoglobin(HbÎ±) dan beta-hemoglobin (HbÎ²). Analisis morfologi menunjukkan bahwa rata-rata ukuran badan dan bobot tubuh subpopulasi kuda Tomohon lebih tinggi (p<0.05) dibandingkan dengan subpopulasi di 3 daerah lainnya (Manado, Minahasa selatan dan Minahasa). Jarak genetik terdekat (2,75) terdapat antara subpopulasi kuda Minahasa dan Minahasa Selatan, sedangakan jarak genetic terjauh (9,02) terdapat antara subpopulasi kuda Tomohon dan Manado. Hasil kedua analisis ini menunjukkan bahwa subpopulasi kuda Tomohon mempunyai hubungan kekerabatan yang jauh dengan ketiga subpopulasi kuda Sulawesi Utara lainnya.Kata kunci: jarak genetik, kuda, morfologi, polimorfisme protein darahDETERMINATION OFÂ GENETIC DISTANCE ON NORTH SULAWESI NATIVE HORSES BASED ON ANALYSIS OF MORPHOLOGYAND BLOOD-PROTEIN POLYMORPHISMABSTRACTThis research aimed to determine genetic distance among subpopulations of North Sulawesi natice horses using analysis of morphology and blood protein polymorphism. The observed subpopulations of native horses included 505 mature horses 2-7 years old and consisted of 310 males (stallion) and 193 females (mare). Morphological analysis was used to determine the size and weight of body. Analysis of blood protein polymorphism was conducted to estimate genetic polymorphism of Albumin (Alb), Post Albumin (PAlb), Transferin (Tf), Post Transferin-1(PTf1), Post Transferin-2 (PTf2), alfa Hemoglobin (HbÎ±) and beta Hemoglobin (HbÎ²). Morphological analysis showed that average size and weight of body in subpopulation of Tomohon horses were bigger (p<0,05) than 3 other ones (Manado, South Minahasa and Minahasa). The nearest genetic distance (viz. 2,75) was between Minahasa and South Minahasa horses, whereas the longest genetic distance was between Tomohon and Manado subpopulations. These analysis indicated that Tomohon horses bad far relationship to 3 other horse subpopulations ini North Sulawesi.

Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line Nensy Tri Putri; Sri Murtini; Niken Ulupi; Isyana Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3029

The Thy1 gene is a member of the immunoglobulin superfamily, which is found in many different animals. This gene encodes a Thy1 protein (CD90) which measures 25–37 kDa. Although the function of the Thy1 gene is unknown, it is thought to play a crucial role in the immunological response, particularly in the activation of T cells. The goal of this study was to find a variation in the Thy1 gene in IPB D2 chickens that might be utilized as candidates for immunoresistance genetic markers. A total of 123 samples from IPB D2 chickens consisting of 38 samples from IPB D2 G0 (basic generation) chickens and 85 samples from IPB D2 G2 (second generation) chickens were used in this study. PCR methods and direct-DNA sequencing were used in this study. Data analysis in this study consisted of genotype frequency, allele frequency, observed heterozigosity, expected heterozygosity, and Hardy-Weinberg equilibrium. The results of the study discovered two SNPs on the intron (g.2139 CT and g.2542 AG). Four haplotypes were created by combining two SNPs. The g.2139 CTand g.2542 AG were polymorphic, but not in the Hardy-Weinberg equilibrium.

Analysis of Meat Mineral Content in Cemani Chicken with Homozygous (Fm/Fm) and Heterozygous (Fm/fm+) Genotypes Rona Saumy Safitry; A B Dharmayanthi; K Kinoshita; T Akiyama; S Darwati; Tatan Kostaman; S Sopiyana; I Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3075

Cemani chicken is an Indonesian native chicken with black hyperpigmentation on feathers, skin, beak, comb, and flesh. Hyperpigmentation in chickens is called Fibromelanosis. Fibromelanosis in Cemani chickens is semi–dominant, producing two genotypes: homozygous (Fm/Fm) and heterozygous (Fm/fm+). Cemani chicken meat’s black color may indicate a higher mineral content than regular chicken meat. The study’s is aims are to detect genotype homozygous (Fm/Fm) and heterozygous (Fm/fm+) mutations and to determine the mineral content of Cemani chickens with homozygous (Fm/Fm) and heterozygous (Fm/fm+) genotypes. In the Cemani chicken population (n = 32), the Fm–specific allele genotype was detected using a Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–RFLP) and the MluI restriction enzyme. The mineral contents of Cemani chicken tested were Fe, Zn, Mn, and Se. The results showed that homozygous Cemani chickens (Fm/Fm) had higher Fe and Zn mineral content. However, the homozygous (Fm/Fm) and heterozygous (Fm/fm+) Cemani chicken were not different in mineral content of Se and Mn. This study found that different genotypes of Cemani chicken had different mineral compositions. In the future, this analysis supports the selection of chicken strains with high antioxidant levels.

Determination of Complete Sequence Mutation of Myostatin Gene in Fast- and Slow-Growing Chicken Isyana Khaerunnisa; Ahmad Furqon; Saiful Anwar; Jakaria Jakaria; Cahyo Budiman; Irma Isnafia Arief; Cece Sumantri; Yong Soo Kim
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.148-158

Myostatin plays a role in inhibiting skeletal muscle growth in vertebrates. This study aimed to investigate the full sequence of the myostatin gene in fast-growing and slow-growing chickens. Fast- and slow-growing chicken models were produced from F2 Kampung x broiler. The full sequence of the myostatin gene was identified using 24 pairs of primers covering about 8,000 bp. mRNA expression analysis of muscle tissue was performed to examine whether the expression levels of myostatin are affected by chicken lines, sex, or muscle type. The results showed 170 mutations in fast- and slow-growing chickens. One hundred and sixty-one of them are novel mutations. A total of five and twenty-two alleles were specific alleles found only in the fast-growing and slow-growing groups of chickens, respectively. There were no differences in amino acids and gene expression levels of myostatin between the fast- and slow-growing chickens. In summary, the results of this study showed that specific alleles for the fast-growing or slow-growing chicken groups were found, suggesting that these specific alleles potentially be used as genetic markers for muscle growth in chickens.

Polymorphisms KIF12 GENE|BfaI and its Association on Carcass Characteristics and Lamb Meat Quality Raudah Putri Ekawati; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri; Asep Gunawan
Buletin Peternakan Vol 46, No 4 (2022): BULETIN PETERNAKAN VOL. 46 (4) NOVEMBER 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i4.77029

One of gene which involves on mediation an antioxidant cascade in beta cells as an intracellular target of excess fat intake is Kinesin Family 12 (KIF12). This study aims to identify the diversity of SNP g. 9617965 C>T KIF12 and association with carcass characteristic and meat quality. The total sample used were 102 ram DNA samples consisted of 10 barbados cross sheep (BCS), 52 javanese thin tail (JTT), 15 jonggol sheep (JS), 15 garut sheep (GS), and 10 compass agrinak sheep (CAS). Identification of the diversity of KIF12|BfaI was conducted using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The results showed that the amplification product was 696 bp. The diversity of the KIF12 gene was found three genotypes namely CC, CT, and TT. The allele frequency in all types of sheep was in Hardy-Weinberg equilibrium. A SNP of the KIF12 was associated (P<0.05) with characteristic of carcass, including length of carcass. The association of KIF12 with meat quality was significant (P<0.05) too associated with pH and water holding capasity (WHC). It could be concluded that KIF12 gene is a potential candidate gene for carcass characteristic and meat quality traits in sheep

Evaluasi Kebutuhan Nutrien dan Suplementasi Zinc untuk Calon Galur Ayam IPB-D2 Fase Pre-layer: Evaluation of Nutritional Requirements and Zinc Supplementation for IPB-D2 Prospective Chicken Lines in the Pre-Layer Phase Fadhilah Dhani Santika Falah; Sumiati; Cece Sumantri; Dwi Margi Suci
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.123-129

This study aimed to evaluate nutrient requirements and zinc supplementation for IPB D-2 pre-layer chicken line candidates on performance, blood profile, and blood malondialdehyde (MDA). This study used 200 of 16 weeks IPB-D2 chickens line candidates reared until 19 weeks of age (pre-layer phase). This study used a completely randomized design (CRD) with 2 treatments and 10 replications. The treatment rations were formulated to fill the chicken nutrient requirements according to Lohman (2020) without the addition of Zinc and with the addition of 120 ppm Zinc. The data were analyzed using the T test. The results showed that the ration supplemented with 120 ppm zinc did not significantly affect performance, but it was able to increase body weight uniformity. The 120-ppm Zinc supplementation significantly (p<0.05) decreased erythrocytes, however it tended to lower blood MDA. In conclusion, 120 ppm Zinc supplementation in the ration is sufficient to meet the nutrient requirement of IPB-D2 chicken line candidates in the pre-layer phase. Key words: IPB-D2 chicken line candidates, Zinc, performance, blood profile, MDA

Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Amri, Fisma Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya Sari Suryanah SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Darodjah Rasad, Siti Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman Tike Sartika TIKE SARTIKA Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum

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