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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak Zuriat Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Science and Technology Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Jurnal Aplikasi Teknologi Pangan Jurnal Ilmiah Fillia Cendekia Building of Informatics, Technology and Science ZIRAA'AH MAJALAH ILMIAH PERTANIAN Indonesian Journal of Electrical Engineering and Computer Science Jurnal Ilmiah Sains Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Animal Production Annales Bogorienses
Cece Sumantri
Department Of Animal Production And Technology, Faculty Of Animal Science, IPB University, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3) Olfa Mega; Cece Sumantri; Irma Isnafia Arief; Cahyo Budiman
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14904

Abstract

Proteases are one of most important and abundant enzymes produced by the biotechnology industry, for scientific, physiological and industrial application and dominates of the whole enzyme market. Lactobacillus plantarum IIA-1A5 is an Indonesian lactic acid bacteria (LAB) isolated from beef Peranakan Ongole cattle. Preliminary analysis on its whole genome sequence indicated that this strain harbours some genes involved in protein degradation and might be promising to be further applied. This study aims to optimize the gene sequence of a lon-like protease of L. plantarum IIA-1A5 for heterologous expression system. The Lon-like gene expression system is made using genes that have been optimized first in silico. pET-28a(+), E. coli BL21(DE3), Nde1 and BamH1 were used in this study as a expression vector, a host and retriction enzyme, respectively. Molecular weight was validated using SDS-PAGE and expasy.org software. The results showed that optimization increased codon adaptation index value (CAI) and GC content to 0.97 and 56.57%, respectively, which were suitable for the E. coli expression system. The Lon-like IIA gene was successfully expressed in the cell cytoplasm by induction of 1 mM Isopropyl -D-1-thiogalactopyranoside (IPTG) at 37 C. As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. Lon-like IIA was successfully expressed in a host cell induced with 1 mM IPTG at 37oC . IPTG induction was performed at the 3rd hour of incubation with OD600 0.59. In addition, Lon-like IIA molecular weight was detected approximately 43 kDa.
Polymorphism of PPM1K Gene and The Association Related to Retail Indonesian Sheep Meat Cuts Suhendra, Muhammad; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri; Cahyo Budiman; Katrin Roosita; Gunawan, Asep
ANIMAL PRODUCTION Vol. 26 No. 1 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.1.216-1

Abstract

The PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent 1K) gene is assumed to associate with retail meat cuts. This study aims to determine the association of the PPM1K gene polymorphisms with retail meat cuts among various breeds of Indonesian sheep. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach was used to determine the polymorphisms of the PPM1K gene in 130 Indonesian rams aged 10-12 months with body weights ranging from 20-25 kg. The association study between PPM1K and retail meat cuts was analyzed using General Linear Model (GLM). The research showed that the PPM1K gene was polymorphic, with three genotypes: AA (458 bp), GG (259 bp and 199 bp), and AG (458 bp, 259 bp, and 199 bp). The PPM1K gene polymorphisms were significantly (P<0.05) associated with breast cut, which is the breast muscle and bone. The GG genotype of the PPM1K gene had a higher value on breast lamb. This result implies that the PPM1K gene could be a potential candidate for marker-assisted selection for improving the lamb quality of Indonesian sheep.
Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia Purwantara, Bambang; Pardede, Berlin Pandapotan; Agil, Muhammad; Supriatna, Iman; Karja, Ni Wayan Kurniani; Arifiantini, R. Iis; Sumantri, Cece
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0404.367-374

Abstract

Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.
Productivity of IPB-D2 and IPB-D3 Chickens with Repeatability of Newcastle Disease Antibody Titer Ratnawati, Diana; Sri Darwati; Murtini, Sri; Sumantri, Cece
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.140

Abstract

ND virus causes mortality in poultry. Passively acquired maternal antibodies inhibit immunoglobulin formation. Repeatability is a genetic parameter that determines the inheritance of traits from elders to chicks. IPB-D2 chickens were selected for disease resistance, and IPB-D3 chickens were selected for weight gain. This study aimed to evaluate the productivity of IPB-D2 and IPB-D3 chickens and assess the inheritance of Newcastle Disease (ND) antibody traits in 36 IPB-D3 and 21 IPB-D2 chickens. The T-test was used to compare the group means of the two chicken breeds. Antibody titer measurements were based on the Geometric Mean Titer (GMT). Estimation of ND titer repeatability using within-class correlation. Fertility and hatchability differed significantly (p < 0.05). ND titer of IPB-D2 chicken and IPB-D3 chicken DOC LOG 2 GMT 1.61 ± 1.10 and 1.34 ± 0.95. The antibody titer of IPB-D2 and IPB-D3 chickens at 14 days of age was 1.02 ± 1.20 and 1.37 ± 0.95. The ND titer value in the egg yolk of IPB-D2 chicken was 4.02 ± 1.94, and in IPB-D3 chicken was 3.64 ± 2.54. The results showed the repeatability value of l antibodies in IPB D-2 chickens and IPB D-3 chickens in DOC 0.49±0.30, 0.42±0.33 and 0.39±0.28; 0.25±0.15, respectively. Fertility and hatchability of IPB-D3 chickens were better than those of IPB-D2 chickens. The yolk ND titer of IPB-D2 chickens was higher than IPB- D3 chicken. The ND titer reciprocity of IPB-D2 chickens was higher than that of the IPB-D3 chickens. Keywords: IPB-D2 Chicken, IPB-D3 Chicken, Newcastle Disease, productivity, repeatability
Immunocompetence Index Performance of the IPB-D3 Candidate Line under Different Rearing Systems Vianisa, Putri; Murtini, Sri; Furqon, Ahmad; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 2 (2025): August 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.02.11

Abstract

IPB-D3 chickens possess strong adaptive potential that enhances immune system performance and resilience, making them promising candidates for developing superior local breeds. However, their health and welfare are largely influenced by rearing methods, and no data are currently available on this topic. Therefore, this study aimed to assess the immunocompetence index of IPB-D3 chickens under different rearing systems by measuring IgY concentrations. A total of 90 IPB-D3 chickens were reared for 12 weeks; 30 samples were taken from each of the intensive and free-range groups for analysis. The observed variables included the environmental temperature and humidity measured in the morning, afternoon, and evening, as well as the IgY concentrations analysed via indirect ELISA. Blood samples from male and female chickens were collected separately. The data were statistically analysed via an independent t test with SPSS version 26. This study revealed that the daytime temperature and humidity were significantly affected (p < 0.05) by the rearing system but not by the morning or evening. The IgY concentrations did not differ significantly (p > 0.05) between the intensive (10.23 µg/mL) and free-range (9.91 µg/mL) systems. In the intensive system, male chickens had an IgY level of 11.38 µg/mL, and female chickens had an IgY level of 9.16 µg/mL; in the free-range system, male chickens had an IgY level of 9.18 µg/mL, and female chickens had an IgY level of 10.64 µg/mL. These results indicate that neither the rearing system nor sex significantly influences the immune response. This study revealed that both male and female IPB-D3 chickens present high and stable IgY concentrations across different rearing systems, reflecting good immunocompetence and adaptability, thereby supporting their potential as superior lines.
Milk Production Estimation Model for Cattle Based on Image Processing using Random Forest, XGBoost, and LightGBM Niswati, Za'imatun; Nurdiati, Sri; Buono, Agus; Sumantri, Cece
Building of Informatics, Technology and Science (BITS) Vol 7 No 2 (2025): September 2025
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/bits.v7i2.7585

Abstract

Milk is a livestock product consumed by individuals of all ages. Therefore, it is essential to increase milk production in Indonesia to meet domestic demand. The growth of dairy cattle populations and milk production has not been able to keep up with rising consumption, resulting in a reliance on imports for most dairy products and their derivatives, with imports steadily increasing over the years. Therefore, alternative solutions are needed to enhance the milk production. One approach is to develop a milk production estimation model to determine the optimal number of dairy cattle to be cultivated by farmers and livestock companies to meet domestic demand. The objective of this study was to create a dairy milk production estimation model through image analysis using the Random Forest, XGBoost, and LightGBM algorithms. The milk production estimation model used in this study used CLAHE for contrast enhancement and VGG-16 for feature extraction. The results showed that XGBoost provided the best performance, explaining 74% of the data variation in the Y variable with a relatively small estimation error of 0.92. After parameter tuning using Grid Search, an improvement was observed, where XGBoost explained 86% of the data variation in the Y variable, and the estimation error decreased to 0.72. Image processing and machine learning technologies are part of precision agriculture that aims to improve the efficiency, productivity, and sustainability of livestock operations.
Growth Hormone Gene Diversity and Quantitative Characteristics of Thin-Tailed Sheep in Muara Tembesi District Batang Hari Regency Pratama, Bondan; Depison, Depison; Gushairiyanto, Gushairiyanto; Gunawan, Asep; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.13

Abstract

Research on thin-tailed sheep (TTS) via quantitative and molecular characterization is essential for obtaining primary data on genetic diversity. The quantitative description includes body weight, weight gain, and body measurements. Molecular characterization is performed directly on structural genes. One gene that affects livestock growth and metabolism is the growth hormone (GH) gene. The commonly used method is PCR-RFLP. The research was conducted in two stages. Field research was conducted to obtain quantitative characteristic data, including body weight, weight gain, and body measurements, as well as blood samples from 62 TTS (31 males and 31 females). Research in the laboratory includes DNA extraction, PCR amplification, and restriction with the Msp1 enzyme. Data analysis included a t test to calculate the differences in body weight, weight gain, and body size between male and female TTS. T2-hotelling was used to compare the morphometric body sizes of the male and female TTS simultaneously, and principal component analysis was used to determine the body shape and size characteristics of the male and female TTS. The results revealed that the male TTS mean body weight, weight gain, and body size were significantly (P<0.05) greater than those of the female TTS. The results of the molecular analysis of the GH gene with the MspI cutting enzyme revealed that it is monomorphic with the ++ genotype. Conclusion: Body weight, weight gain, and body size were greater in males with TTS than in females with TTS. The body size characteristics of male and female TTS patients were body length, shoulder height, and chest circumference. The body shape characteristic was chest depth. The restriction of the GH|Msp1 TTS gene DNA fragment in exon 5 in the Muara Tembesi district is monomorphic.
Effects of Supplementation with Different Nutrient Contents of Vitamin E Diets on the Performance and Health Status of IPB-D3 Candidate Chicken Strains Romantis, Sara; Sumiati; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.13

Abstract

The purpose of this study was to evaluate the effect of supplementation with 200 ppm vitamin E in a diet with different nutrient contents on the performance and health status of the IPD-D3 candidate chicken strain. This research used 160 IPB-D3 chickens that were reared for 5 to 12 weeks. This experiment used a completely randomized factorial design with 4 treatments and 4 replications. P0 = control diet, P1 = control diet minus 3% ME and 3% PK, P2 = P0 + vitamin E 200 ppm, P3 = P1+ vitamin E 200 ppm. The data were analysed using ANOVA with an interaction’s significant difference test. The results showed that there was an interaction effect between supplementation with 200 ppm vitamin E and different nutrient contents on feed consumption (P<0.05). Supplementation of vitamin E (200 ppm) in the control diet according to Cobb’s recommendation gave the highest feed consumption. A decrease in the metabolizable energy (90,50 kcal kg-1) and crude protein (7.2%) of the diet (P1) significantly decreased (P<0.05) the final body weight, body weight gain and feed efficiency. Supplementation with 200 ppm vitamin E significantly (P<0.05) decreased the leukocyte count. In conclusion, reducing the metabolic energy and protein content of the diet reduced chicken growth by 10.40% and feed efficiency by 7.24%. Supplementation of vitamin E in the diet has a good effect on immunity and reduces leukocytes. According to the Cobb broiler standard (2018), the IPB-D3 chicken strain still responds to a diet that contains nutrients supplemented with 200 ppm vitamin E, which results in good performance and production
Identifikasi Keragaman Gen Myostatin (MSTN|BsrI) Pada Ayam Kampung Dengan Metode PCR-RFLP Shidiq, Fajrin; Yulianto, Yulianto; Gunawan, Asep; Sumantri, Cece
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 24 No. 1 (2023): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/jtapro.2023.024.01.8

Abstract

Myostatin (MSTN) atau Growth and Differentiation Factor 8 (GDF8) adalah anggota dari superfamili Transforming Growth Factor (TGF)-β yang bertindak sebagai pengatur negatif pertumbuhan otot rangka. Mutasi pada ekson 2, di mana basa timin pada gen myostatin berubah menjadi basa guanin, mengubah asam amino leusin menjadi arginin, yang mempengaruhi berat badan ayam. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen myostatin pada 132 ekor ayam kampung yang terdiri dari ayam kampung Ciawi dan kampung Sukabumi. Sebagai perbandingan keragaman pada populasi besar digunakan ayam broiler, nunukan dan petelur. Keragaman gen diidentifikasi menggunakan metode Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi BsrI. Produk amplifikasi sepanjang 247 pb, genotipe yang ditemukan pada keragaman gen MSTN|BsrI adalah GG, GT dan TT. Hasil analisis menunjukkan bahwa frekuensi genotipe GT dan TT lebih tinggi dibandingkan dengan genotipe GG. Pada semua populasi frekuensi alel T lebih besar dari frekuensi alel G yaitu 0.72 dan 0.28. Gen MSTN|BsrI pada populasi ayam kampung Ciawi dan ayam broiler berada dalam kesetimbangan Hardy-Weinberg. Populasi ayam kampung Ciawi dan Sukabumi menunjukkan skor heterozigositas (Ho) lebih tinggi dari skor heterozigositas yang diharapkan (He), sedangkan ayam pedaging menunjukkan skor heterozigositas (Ho) sama dengan skor heterozigositas yang diharapkan (He). Gen MSTN|BsrI pada populasi Kampung Ciawi, Kampung Sukabumi dan ayam broiler bersifat polimorfik
PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation) Hidayat, Numan; Sumantri, Cece; Afnan, Rudi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5091

Abstract

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.081.44% and 80.821.30%) which were significantly higher (P0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.170.56% and 4.650.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.
Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Amri, Fisma Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman TIKE SARTIKA Tike Sartika Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum