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Keragaman Gen K-Kasein dan Hubungannya dengan Produksi dan Kualitas Susu pada Domba di Unit Pendidikan dan Penelitian Peternakan (UP3) Jonggol Cece Sumantri; Eryk Andreas; Achmad Farajallah; Jarmuji Jarmuji
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 1 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objectives of this study were to identify polymorphisms in ovine K-casein gene exon 4 and its association with milk yield, percentage of protein and milk fat in sheep under extcnsif management at JASTRU (Jonggol Animal Studies Teaching and Research Unit) farm, Fact. of Anim Sci, Bogor Agric. University. The 83 blood sample from ewes which have record of milk production were used to determined polymorphism by using PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) methode. The research activities were carried out through blood collection, DNA isolation, and fragment DNA amplification by PCR and separation in electrophoresis with silver staining. Electrophoretic pattern (PCR-SSCP) revealed th rce types of K-casein gene were dcsignitcd A, Band C type. The highest proportion was A type 69.57% and followed hy B 28.99% and the lowest C 1.45%. The were no significant effect of types of of K-casein gene on milk production and milk quality (percentage of protein and fat) in local sheep from Jonggol.   Keywords: .Jonggol sheep, K-cascin gcn, PCR-SSCP, polymorphism
KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL Cece Sumantri; . Jakaria; Mohamad Yamin; Henny Nuraini; Bramada Winiar Putra; Eryk Andreas
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12).
Polimorfisme g.-371T>A Promotor Gen Miostatin pada Sapi Pedaging Indonesia Sutikno Sutikno; Rudy Priyanto; Cece Sumantri; Jakaria Jakaria
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.116 KB) | DOI: 10.18343/jipi.25.2.239

Abstract

Myostatin (MSTN) gene acts as a negative regulator of muscle growth. The aim of the present study was to identify polymorphism of g.-371T>A in promoter region of MSTN gene in Indonesian beef cattle. Blood samples were collected from 191 cattle, including Bali (BL) (42), Madura (MD) (20), Pesisir (PI) (17), Katingan (KT) (16), Ongole grade (PO) (22), Pasundan (PD) (14), Sumba ongole (SO) (10), Brahman (BH) (17), Simmental (SM) (15), and Limousin (LM) (18). Polymorphism of MSTN gene was analyzed using PCR-RFLP (DraI) and direct sequencing methods. Results of genotyping g-371T>A were polymorphic (TT, TA, and AA genotypes) in Simmental, SO, and Katingan. The frequencies of alleles T and A were 0.83; 0.90; 0.97 and 0.17; 0.10; 0.03 respectively. The values of Ho and He were 0.06–0.20 and 0.06–0.28, respectively. The results of this study are in Hardy-Weinberg equilibrium (P> 0.05). Whereas in PD, MD, PI, PO, BH, LM, and BL were monomorphic (TT genotype). The result of sequencing the promoter region of MSTN gene found that mutations transversion was occurred in T to A at g.-371. It was concluded that g.-371T>A of MSTN gene was polymorphic which was potential to be used as genetic markers of muscle growth in SM, SO, and KT cattle. Keywords: SNP g.-371T>A of MSTN gene, Sumba ongole cattle, Katingan cattle, Simmental cattle
Sifat-Sifat Kuantitatif dan Jarak Genetik Kerbau Lokal Sulawesi Tenggara Berdasarkan Pendekatan Morfologi Muh. Rusdin; Dedi Duryadi Solihin; Asep Gunawan; Chalid Talib; Cece Sumantri
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 3 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.318 KB) | DOI: 10.18343/jipi.23.3.203

Abstract

Information on animal genetic resource is needed in conservation program of Indonesian local buffalo. This study was aimed to analyze the quantitative traits diversity and genetic distance of local buffalo of Southeast Sulawesi local based on morphological approach. The total of 271 heads of swamp buffaloes were used in the study from four sub-populations, namely Bombana island’s, Bombana mainland, Kolaka and Konawe. Duncan Multiple Range test at 5% probability was used to compare the quantitative traits between the buffalo sub-populations. Genetic distance was analyzed by using the simple discriminant function. The results showed that the average body weight of male buffalo between sub-populations was not significantly different (P>0.05), but the average body weight of females buffalo from Bombana Island’s (465.22 ± 103.25) was significantly (P<0.05) higher than those of the other sub-populations. The highest variation of quantitative traits of local buffalo was found in Konawe sub-population, i.e. in body weight of female buffalo (35.36%) and body weight of male buffalo (22.05%), rump length in males buffalo (20.44%), and rump width in females buffalo (14.58%). Southeast Sulawesi local buffalo can be grouped into three clusters based on the phylogeny tree, namely 1) Kolaka and Konawe cluster, 2) Bombana island’s cluster, and 3) Bombana mainland cluster. The distinguishing variables of the group of Southeast Sulawesi local buffalo were rump length, body length, and chest circumference. The highest phenotype similarity value was shown by Bombana island’s buffalo (68.57%), while the lowest was found at Konawe buffalo (26.76%). The farthest genetic distance was found between Bombana Islands and Konawe buffalo (3.71703), while the closest genetics distance was identified between Kolaka and Konawe buffalo (0.86616).
Analisis Keterkaitan GEN BMP15, BMPR1B, dan KISS1 dengan Sifat Fekunditas pada Kambing Peranakan Etawah Betina Rini Herlina Mulyono; Cece Sumantri; Ronny Rachman Noor; . Jakaria; Dewi Apri Astuti
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.746 KB) | DOI: 10.18343/jipi.24.2.83

Abstract

Etawah grade goat is a result of grading up line between Kacang and Etawah goats, which well adapted to Indonesia’s humid tropical climate. This goat is a dual-purpose goat breed for milk and meat production. The objectives of this study were to evaluate the polymorphism of fecundity genes through the investigation of four candidate genes for prolificacy, i.e., BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) and their associations with the litter size of Etawah-Grade does. The diversity of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (introns 1) genes were analyzed by the PCR-RFLP technique. A total of 106 of DNA samples were collected from Etawah-Grade does at BPTU-HPT Pelaihari in Pelaihari, South Kalimantan (51 heads) and Cordero Farm in Bogor, West Java (55 heads). Genetic polymorphisms between the two Etawah-Grade groups were calculated as well as their allele and genotype frequencies and the Polymorphic Informative Content (PIC). The Kruskal-Wallis one-factor ranked analysis of variance was used to determine whether there was any effect of genotype of the fecundity genes on litter size. The polymorphic information content (PIC) estimated at BPTU-HPT Pelaihari was 0.313, whereas at Cordero farm was 0.174, and overall PIC was 0.244. There was no association between genotypes of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) genes and litter size. Results indicated that BMP15 (exon 1) and KISS1 (exon 1) genes were monomorphic. However, the other two loci showed polymorphisms. The BMPR1B (exon 1) locus had two genotypes with the frequency for CC (96.23%) and for GG (3.77%), and KISS1 (intron 1) locus had three genotypes with the frequency for TT (80.19%), AT (17.92%), and AA (1.89%).
Respons Fisiologis Ayam KUB (Kampung Unggul Balitbangtan) dan Ayam Walik dengan Haplotipe Gen HSP70 Berbeda yang Terpapar Cekaman Panas Akut Any Aryani; Dedy Duryadi Solihin; Cece Sumantri; Rudi Afnan; Tike Sartika
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.276

Abstract

This study was conducted to determine the physiological response of acute heat stress in Kampung Unggul Balitbangtan (KUB) and Walik chickens with four different HSP70 gene haplotypes (H1', H1, H2, and H3). Acute heat stress is carried out at 35°C for one hour. The data was analyzed based on rectal temperature measurements, distribution of body surface temperature (head, neck, body, and leg area), hormone levels (corticosterone and triiodothyronine), and time of panting. Body surface temperature is detected using infrared thermography. The results showed that haplotype H1 Walik chicken is more susceptible to heat stress based on the rectal temperature. In contrast, H1' haplotype Walik chicken released more heat through the body surface in the leg area. Keywords: heat stress, hormone, KUB chicken, infrared thermography, walik chicken
KARAKTERISTIK MINUMAN WHEY YANG DIFERMENTASIKAN DENGAN BAKTERI ASAM LAKTAT INDIGENUS ASAL DANGKE Setiawan Putra Syah; Irma Isnafia Arief; Epi Taufik; Cece Sumantri
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 2 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.867 KB) | DOI: 10.6066/jtip.2017.28.2.129

Abstract

Whey from the dangke processing center in Enrekang district, South Sulawesi, is the main by-product which can contribute to environmental pollution. On the other hand, the nutritional content of whey is highly potential to be developed into high value food products and provide functional benefits. Whey processing into a fermented drink is one of the easy and inexpensive alternatives with good prospect to be developed. The aim of this study was to investigate the microbial, physical, chemical and sensory characteristics of the whey drink fermented (FWD) by probiotic lactic acid bacteria (LAB) isolated from dangke. The LAB strain used were Lactobacillus fermentum strains B323K, C113L, A323L, C222L, and B111K. The results showed that the LAB strains grew well the whey medium and the viability met the standard of dairy fermented product. FWD have similar pH, % titratable acidity, and aW characteristics in all fermentation treatments with LAB strains from dangke, however to adjust the pH and acidity level of FWD to the yoghurt standard (pH 4.4 and %TAT 0.9–1.2%), the FWD fermentation must be stopped at the 20th hour. Changes in the level of moisture, ash, and carbohydrate contents of FWD occurred after the fermentation. The sensory quality tests showed that the FWD fermented by LAB strains from dangke was preferred by the panelists than the unfermented one (Whey + 8% sucrose).
Kualitas Spermatozoa dalam Modifikasi Pengencer Ringer Laktat Kuning Telur dengan Tambahan Astaxanthin dan Glutathione pada Tiga Jenis Ayam Lokal Nila Pratiwi; Tuty Laswardi Yusuf; Iis Arifiantini; Cece Sumantri
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.149 KB) | DOI: 10.29244/avi.7.1.46-54

Abstract

Tujuan penelitian ini adalah untuk menguji efek antioksidan astaxanthin dan glutathione dalam pengencer Ringer Laktat Kuning Telur (RL-KT) pada kualitas semen cair ayam merawang, SK kedu, dan kampung. Masing-masing 5 ekor ayam merawang, SK kedu, dan kampung, berumur 1-1,5 tahun digunakan sebagai sumber semen. Penelitian ini terdiri atas 2 tahap: (I) penelitian pertama bertujuan untuk menentukan dosis terbaik antioksoidan astaxanthin (0,004 %, 0,005 %) dan glutathione (0,007%, 0,008 %) pada motilitas spermatozoa dengan menggunakan lima ekor ayam merawang. (II) penelitian kedua bertujuan menguji dosis antioksidan terbaik yang didapatkan dari penelitian pertama pada motilitas dan viabilitas spermatozoa pada 3 jenis ayam lokal. Hasil penelitian pertama menunjukkan tidak ada perbedaan pengaruh antara dosis astaxanthin dan glutathione dalam pengencer RL-KT yang disimpan selama 60 jam, namun spermatozoa pada dosis astaxanthin 0,004% (3900±3,3%) menunjukkan motilitas yang lebih baik sehingga digunakan pada penelitian kedua. Hasil penelitian kedua menunjukkan bahwa penambahan astaxanthin 0,004% dapat mempertahankan motilitas spermatozoa yang disimpan sampai 36 jam pada ayam SK kedu (40,10±1,33%) dan kampung (41,03±2,44%) dan 24 jam pada ayam merawang (46,41±4,42%). Viabilitas spermatozoa pada ketiga jenis ayam tergolong tinggi, yaitu berkisar antara 59,37±5,65% sampai 65,35±6,25%. Dari data yang diperoleh, volume dan konsentrasi spermatozoa ayam merawang lebih tinggi dibandingkan SK kedu dan kampung. Semen diketahui mampu bertahan dalam waktu 36 jam pada pengencer RL-KT dengan penambahan astaxanthin 0,004% dengan motilitas mencapai 40%. Pengencer RL-KT yang ditambahkan astaxanthin 0,004% menghasilkan rata-rata fertilitas spermatozoa sebesar 87%.
Performa dan Karakteristik Semen serta Freezability Spermatozoa Ayam IPB-D1 dengan Konsentrasi Immunoglobulin Yolk Berbeda Yusman Setiawan; Niken Ulupi; Cece Sumantri; R. Iis Arifiantini
Acta VETERINARIA Indonesiana Vol. 9 No. 1 (2021): Maret 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.1.36-43

Abstract

Ayam IPB-D1 merupakan jenis ayam lokal dari hasil persilangan antara jantan F1 PS (Pelung x Sentul) dengan betina F1 (Kampung x Ras pedaging paren stock Cobb). Penelitian ini bertujuan untuk mengevaluasi performa, kualitas semen, dan freezability spermatozoa ayam IPB-D1 pada konsentrasi Immunoglobulin Yolk (IgY) berbeda. Materi yang digunakan yaitu ayam IPB-D1 jantan sebanyak 20 ekor, terdiri atas 10 ekor ayam dengan konsentrasi IgY rendah dan 10 ekor IgY tinggi dengan ayam umur 8 bulan. Variabel yang diamati yaitu performa yakni konsumsi pakan, pertambahan berat badan, morbiditas dan mortalitas, kualitas semen segar dan kualitas semen beku (freezability). Data dianalis menggunakan uji-t menggunakan program SAS dengan membandingkan antara konsentrasi IgY tinggi dan IgY rendah. Hasil analisis performa ayam IPB-D1 tidak berbeda (P>0.05) terhadap IgY yang berbeda. Hasil kualitas semen menunjukkan perbedaan yang signifikan terhadap konsistensi semen dan motilitas, viabilitas, konsentrasi serta konsentrasi spermatozoa per ejakulat. Ayam IPB D-1 pada dua konsentrasi IgY tidak berbeda nyata (P>0.05) terhadap motilitas semen segar dan recovary rate, namun Ayam IPB D-1 dengan konsentrasi IgY tinggi memiliki motilitas spermatozoa pasca thawing lebih baik daripada (P<0.05) Ayam dengan konsentrasi IgY yang rendah.
Keragaan Sifat Kualitatif dan Kuantitatif Kerbau Lokal di Propinsi Banten (Performance of Qualitative and Quantitative Traits of Local Buffaloes at Banten Province) Dudi Syadili; Cece Sumantri; Harimurti Martojo; Asep Anang
Jurnal Ilmu Ternak Vol 11, No 2 (2011)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v11i2.371

Abstract

Penelitian ini bertujuan untuk mengetahui sifat kualitatif dan kuantitaif kerbau lokal.  Metode penelitian yang digunakan adalah survey, penentuan sampel berdasarkan purposive sampling. Lokasi penelitian adalah Kabupaten Serang, Pandeglang dan Kabupaten Lebak Propinsi Banten. Pengambilan data dilakukan melalui observasi dan pengukuran. Jumlah kerbau yang diteliti sebanyak 300 ekor terdiri atas 150 ekor kerbau jantan dan 150 ekor kerbau betina dewasa. Data dianalisis menggunakan analisis statistik deskriptif dan sidik ragam. Hasil penelitian diperoleh  sifat kualitatif untuk  warna kulit dan kaki adalah abu-abu gelap, bentuk tanduk umumnya melingkar ke  bawah, garis kalung ganda,  unyeng-unyeng ditemukan pada kepala, pundak dan pinggul.  Ukuran-ukuran tubuh  kerbau jantan dan betina  berturut-turut: lingkar dada (cm) 171,98±5,00; 161,33±4,83; dalam dada (cm) 65,13±3,97; 59,53±5,76; panjang badan (cm) 122,10±6,40; 120,00±2,03; tinggi pundak (cm) 130,02±2,80; 118,99±3,20;  tinggi pinggul (cm) 103,42±2,27; 105,28±2,87; lebar pinggul (cm) 32,50±1.61; 31,41±1,39;  panjang kepala (cm) 41,99±1,42; 41,39±1,55; lebar kepala (cm) 20,21±1.83; 19,30±1,15; lebar ambing (cm) ; 47,15±5,20; lebar teracak (cm) 15,89±1,77; 14,84±1,07; dan lingkar skrotum (cm) 15,88±0,67. Tidak terdapat perbedaan yang nyata (P>0,05) ukuran  tinggi pundak, tinggi pinggul, panjang kepala, lingkar skrotum serta lebar teracak pada kerbau jantan dan betina pada subpopulasi Serang, Pandeglang dan Lebak, akan tetapi terdapat perbedaan yang nyata (P<0,05)  ukuran lebar pinggul, panjang badan, lingkar dada, dalam dada, dan lebar kepala. Kerbau yang berada di lokasi penelitian dari kajian sifat kualitatif dan kuantitatif dapat digolongkan sebagai kerbau lumpur.Kata Kunci:  keragaan sifat kualitatif, sifat kuantitatif, kerbau lokal
Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Fisma Amri Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman TIKE SARTIKA Tike Sartika Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum