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Association of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattle Dairoh Dairoh; Jakaria Jakaria; Mokhamad Fakhrul Ulum; Andi Baso Lompengeng Ishak; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 2 (2021): June 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i2.2701

Abstract

Bali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that  SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.
Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken Lailatul Rohmah; Sri Darwati; Niken Ulupi; Isyana Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2525

Abstract

The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.
Single nucleotide polymorphism of prolactin gene exon two in ducks of Pekin, Mojosari and Pekin Mojosari crossbred Irma .; Cece Sumantri; Triana Susanti
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 2 (2014): JUNE 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i2.1038

Abstract

Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc.
Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province Cece Sumantri; R Diyono; A Farajallah; A Anggraeni; E Andreas
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 4 (2010): DECEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2820.571 KB) | DOI: 10.14334/jitv.v15i4.668

Abstract

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo
Detection of deletion and mutation on pig Mx1 gene (gene resistance to influenza virus) with PCR-RFLP Nar I restriction Cece Sumantri; T Morzumi; N Hamashima
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.075 KB) | DOI: 10.14334/jitv.v6i1.216

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The study was done to detect the incident of deletion and mutation in exon 14th of Mx1 gene in pig. Six hundred base pairs at the position (1937 to 2537) of the 14th exon of the pig Mx1 gene was amplified by polymerase chain reaction (PCR) from 15 breed of pig DNA sample. The amplified PCR products were digested by Nar I enzyme that called restriction fragment length polymorphism (PCR-RFLP) technique. The results show genetic polymorphism at the 14th exon of pig Mx1 gene. The Nar I digested revealed three phenotyphic variation (C/C, C/N and N/N, designated for Nar I cut homozygote, heterozygote and for Nar I no cut homozygote, respectively). The Nar I (N/N or C/N) type is corresponding to (1) the deletion 11 bp at the position 2064 to 2075. This type was observed in Landrace breed. (2) the incidence of two point mutation at the position 2065 Guanine (G) change to Thymine (T) and at the position 2124 Guanine (G) change to Adenine (A).This type was observed in Chinese native pig (Meishan) and Vietnamese native pig.   Key words: Deletion, mutation, pig Mx1 gene and PCR-RFLP
Isolation and identification of indigenous lactic acid bacteria from North Sumatra river buffalo milk Heni Rizqiati; Cece Sumantri; Ronny Rachman Noor; E. Damayanthi; E. I. Rianti
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.693 KB) | DOI: 10.14334/jitv.v20i2.1163

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Buffalo milk is a source of various lactic acid bacteria (LAB) which is potential as culture starter as well as the probiotic. This study was conducted to isolate and identify LAB from indigenous North Sumatra river buffalo milk. Lactic acid bacteria was isolated and grown in medium De Man Rogosa Sharpe Agar (MRSA). The isolation was conducted to obtain pure isolate. The identification of  LAB was studied in terms of morphology, physiology, biochemistry and survival on low pH. Morphology tests were conducted by Gram staining and cell forming; physiology tests were conducted for growing viability at pH 4.5 and temperature at 45oC; whereas biochemistry tests were conducted for CO2, dextran and NH3 productions. Determination of LAB species was conducted using Analytical Profile Index (API) test CHL 50. Results of identification showed that 41 isolates were identified as LAB with Gram-positive, catalase-negative, rod and round shaped characteristics. Resistance test done to low pH (pH 2) for the lactic acid bacteria showed decrease of bacteria viability up to1.24±0.68 log cfu/ml. The resistant isolates at low pH were L12, L16, L17, L19, L20, M10, P8, S3, S19 and S20. Identification with API test CHL 50 for 10 isolates showed that four isolates were identified as Lactobacillus plantarum, L. brevis, L. pentosus and Lactococuslactis.
Gene Controlling High Milk Lactoferrin Content in Holstein Friesian Cows Cece Sumantri
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.262 KB) | DOI: 10.14334/wartazoa.v16i2.861

Abstract

Lactoferrin is considered as an antimicrobial and antiviral protein, therefore milk that contains high lactoferrin is potential for health-enhancing nutraceuticals for food and pharmaceutical applications . The main objective of this paper is to discuss in more detail about lactoferrin function and how gene could control high lactoferrin content in milk . Milk lactoferrin content is still various among individual . Therefore, selection should be carried out to increase lactoferrin content in local Friesian Holstein . The selection could be accelerated by using lactoferrin gene as a gene marker that directly contributes to the milk lactoferrin content . Key words : Lactoferrin, antimicrobial, antiviral, selection, gene marker
Karakterisasi Gen KIF12 (Kinesin Familly 12) serta Hubungannya dengan Komposisi Asam Lemak pada Domba Asep Gunawan; Sarah Tazkya; Kasita Listyarini; Mohamad Yamin; Ismeth Inounu; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.389 KB) | DOI: 10.33772/jitro.v5i3.4978

Abstract

ABSTRAKKinesin Family 12 (KIF12) merupakan gen yang terlibat dalam mediasi kaskade antioksidan dalam sel beta sebagai sebuah target intraselular dari kelebihan asupan lemak atau lipotoksit. Penelitian ini bertujuan untuk mengidentifikasi karakterisasai gen KIF12 melalui identifikasi keragaman dan asosiasi gen KIF12 terhadap komposisi asam lemak pada domba. Sampel domba yang digunakan sebanyak 35 ekor yang terdiri dari Domba ekor Gemuk (DEG) dan Domba Ekor Tipis (DET) masing-masing 20 dan 15 ekor. Titik mutasi gen KIF12 berada pada posisi g.9617965 C>T.  Identifikasi keragaman gen KIF12 dianalisis menggunakan Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) dengan menggunakan enzim BfaI. Hasil keragaman gen KIF12 bersifat polimorfik dengan ditemukan tiga genotipe yaitu CC, CT, dan TT. Hasil Uji chi square menunjukkan bahwa lokus KIF12 berada pada keseimbangan Hardy-Weinberg (HWE) pada pada DET, hal berbeda ditunjukakan pada DEG. Titik mutasi gen KIF12 berasosiasi secara signifikan  (P <0.05) terhadap asam lemak jenuh (SFA)  asam laurik (C12:0), asam miristik (C14: 0) and asam heptadenoik (C17: 0) juga asam lemak jenuh tunggal (MUFA), asam miristoleinik (C14: 1) and asam oleik (C18: 1n9c). Gen KIF12 memiliki potensi untuk digunakan sebagai penanda seleksi terhadap komposisi asam lemak.Kata kunci:  domba, asam lemak, KIF12, PCR RFLPABSTRACTKinesin Family 12 (KIF12) is one of gene which involved in mediates an antioxidant cascade in beta cells as an intracellular target of excess fat intake or lipotoxicitys. This study was aimed to identify the polymorphism and association of KIF12 gene as candidate gene for fatty acid composition in Indonesian sheep including fat-tailed sheep and thin-tailed sheep. The sample of sheeps used 35 heads consist fat-tailed sheep and thin-tailed sheep as many as each 20 and 15 samples. Identification of polymorphism of KIF12|BfaI gene were analyzed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). The results of polymorphism of KIF12 gene were polymorphic for three kind of genotypes of CC, CT, and TT. The chi square revealed that the locus of KIF12 was deviated in Hardy-Weinberg equilibrium (HWE) in fat-tailed sheep, but was in HWE in thin-tailed sheep. A SNP of KIF12 was associated (P <0.05) with saturated fatty acids (SFA) including lauric acid (C12:0), myristic acid (C14: 0) and heptadecanoic acid (C17: 0) as well as monounsaturated fatty acids (MUFA), namely miristoleinic acid (C14: 1) and oleic acid (C18: 1n9c).The KIF12 gene was the potential to be used as a marker of selection for fatty acid compositions.   Keywords : sheep, fatty acids, KIF12, PCR-RFLP
Pertumbuhan Bulu Ayam Hasil Persilangan Sentul dengan Onagadori dan Resiprokal Umur 1 sampai 12 Minggu Achmad Gitaraka Archimilar; Sri Darwati; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.739 KB) | DOI: 10.33772/jitro.v6i1.5574

Abstract

ABSTRAKAyam tidak hanya sebagai komoditi pangan, namun juga hias. Salah satu ayam hias yang unik dan banyak menarik minat pecinta ayam hias adalah ayam ekor panjang atau onagadori. Salah satu jenis asli ayam lokal Indonesia adalah ayam sentul. Ayam sentul adalah ayam asli dari daerah Ciamis, Jawa Barat. Persilangan onagadori dengan sentul dan resiprokalnya diharapkan memiliki bulu yang panjang di bagian tubuhnya dengan perpaduan warna bulu abu-abu akibat adanya kombinasi warna dari kedua tetua dengan performa tubuh yang gagah dan indah. Parameter yang diukur dalam penelitian ini adalah pertumbuhan bulu sayap, pinggang, dan ekor pada umur 1-12 minggu. Pada penelitian ini digunakan rancangan acak kelompok untuk menganalisa data. Hasil penelitian menunjukan bahwa panjang  bulu sayap SO jantan 7,93±0,63 cm, SO betina 7,96±0,79 cm dan  OS jantan  6,39±0,66 cm, OS betina 6,29±0,91 cm. Hasil pengukuran bulu pinggang SO jantan 6.80±0.10 cm, SO betina 6,81±0,10 cm dan OS jantan 2,59±0,86 cm, OS betina 2,50±0,98 cm. Panjang bulu ekor SO jantan 10,71±0,10 cm, SO betina 10,78±0,10 cm dan OS jantan 7,31±0,10 cm, OS betina 7,29±0,12 cm. Karakteristik kualitatif ayam hasil persilangan onagadori dengan sentul pada warna bulu jantan cenderung lebih dominan 1 warna sedangkan yang betina 2 warna. Warna shank yang dihasilkan dari persilangan ini adalah abu dan putih lebih banyak dibandingkan hijau. Bentuk jengger hasil persilangan ini bentuk jengger pea lebih banyak daripada bentuk jengger single dan cushion. Ayam hasil persilangan SO memiliki pertambahan pertumbuhan bulu sayap, pinggang, dan ekor lebih cepat dan panjang dari OS.Kata kunci: onagadori, pertumbuhan bulu, resiprokal, sentulABSTRACTChicken is not only a food commodity, but also ornamental. One of the unique ornamental chickens that attracts many ornamental chicken lovers is the long-tailed chicken or onagadori. One native of Indonesian local chicken is sentul chicken from the Ciamis area, West Java. The crossing of cock onagadori with hen sentul (Onagadori-Sentul/OS) and its reciprocal cock sentul with hen onagadori (Sentul-Onagadori/SO) is expected to have long feather tail, and on its body with a combination of gray feather due to the combination of colors from the two parents with a good performance.. Parameters measured in this study were the growth of wing, saddle, and tail feathers at 1-12 weeks. A randomized block design is used to analyze this data.  The measurement of the males 6.80±0.08 cm, SO females 6.81±0.08 cm, and OS males 2.59±0.86 cm, Females OS 2.50±0.98 cm. Result of SO tail tailed data 10.71±0.10 cm, SO females 10.78±0.10 cm and OS males 7.31±0.10 cm Females OS 7.29±0.12 cm. Chicken offspring from SO crossbreed has better feather growth than onagadori. While the offspring from OS crossbreed has better feather growth than sentul chicken. Characteristics of qualitative chicken onagadori crosses with sentul on the color of male feathers tend to be more dominant one color while the female two colors. The shank color resulting from this cross color of the ash and white shank is more dominant than green. The pea comb form produced from this cross is more dominant than the single and cushion comb. The SO cross breed chikens have faster feather growth of wing, saddle, and tail than OS chikens.Key words: feathers growth, onagadori,  reciprocal, sentul
Identifikasi Keragaman Gen DGAT1 serta Asosiasinya terhadap Karakteristik Karkas dan Sifat Perlemakan Domba Asep Gunawan; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 2 (2019): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.913 KB) | DOI: 10.33772/jitro.v6i2.7141

Abstract

ABSTRAK Karakteristik karkas dan sifat perlemakan pada daging domba dikontrol oleh banyak gen salah satunya gen DGAT1 (Diacylglycerol Acyltransferasel 1). Penelitian ini bertujuan mengidentifikasi SNP (Single Nucleotide Polymorphism) gen DGAT1 pada titik mutasi g.8539 C>T dan asosiasinya terhadap karakteristik karkas dan sifat perlemakan pada domba Indonesia. Total sampel domba yang digunakan sebanyak 150 buah terdiri dari 35 sampel domba compass agrinak (DCA), 36 sampel domba barbados cross (DBC), 41 sampel domba komposit garut (DKG), 20 sampel domba ekor gemuk (DEG), dan 18 sampel domba ekor tipis (DET). Karakteristik karkas dan sifat perlemakan diukur dari domba jantan berumur 10-12 bulan. Identifikasi keragaman DGAT1|ALuI dianalisis dengan metode PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Hasil keragaman gen DGAT1 bersifat polimorfik dalam DET dan DEG, sedangkan DCA, DBC, dan DKG bersifat monomorfik. Dua genotipe disebut CC dan  CT ditemukan dalam DET dan DEG. Titik mutasi gen DGAT1 berasosiasi (P<0.05) dengan karakteristik karkas, yaitu bobot dan panjang karkas. Selain itu, keragaman gen DGAT1 juga berasosiasi signifikan (P<0.05) dengan asam lemak jenuh, yaitu asam stearat (C18:0) dan asam arakidat (C20:0) dan asam lemak tak jenuh tunggal, yaitu asam oleat (C18:1n9c). Gen DGAT1 memiliki kontribusi dalam karakteristik karkas dan komposisi asam lemak pada domba.Kata Kunci: domba, gen DGAT1, karakteristik karkas, PCR-RFLP, sifat perlemakan                                                              ABSTRACT            Characteristic of carcass and fatness traits of sheep is regulated by many genes such as DGAT1 (Diacylglycerol Acyltransferasel 1) gene. The research was aimed to investigate SNP (Single Nucleotide Polymorphism) of DGAT1 and its association with characteristic of carcass and fatness traits in Indonesian sheep. A total sample of sheeps used 150 rams of 10–12 months consisted 35 samples of compas agrinak sheep (CAS), 36 of barbados cross (BCS), 41 of garut composite (GCS), 20  of javanese fat tailed (JFT), and 18 of javanese thin tailed (JTT). Identification variant of DGAT1|ALuI were performed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The results of polymorphism of DGAT1 were found in JTT and JFT. However, SNP of DGAT1 in CAS, BCS and GCS were monomorfic. Two genotype namely CC and CT were found in JTT and JFT populations. A SNP of the DGAT1 was associated (P<0.05) with characteristic of carcass, including weight and length of carcass. The variant of DGAT1 was associated too with saturated fatty acids (SFA) including stearic acid (C18:0) and arachidic acid (C20:0), and mono unsaturated fatty acid (MUFA) including oleic acid (C18:1n9c). The DGAT1 gene was contribute to characteristic carcass and fatty acid composition in sheep.Keywords: DGAT1 gene, characteristic carcass, fatness traits, PCR-RFLP, sheep
Co-Authors . Hidayati . Subandriyo A Anggraeni A Anggraeni A B Dharmayanthi A Gunawan A M Fuah A.B.L. Ishak A.B.L. Ishak Abdul Samad ACHMAD FARAJALLAH Achmad Gitaraka Archimilar Adi Teruna Effendi Agus Buono Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Romadhoni Surya Putra ahmad yani Akbanugra Yudhananda Al Mukhlas Fikri Aldri Frinaldi Amalia Rahma Aminuyati Andi Baso Lompengeng Ishak Anggraeni Anggraeni Anggraeni, A. Anggraeni Ani Rosmayanti Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Anneke Anggraeni Any Aryani Arief Boediono Aron Batubara Asep Anang Asep Gunawan ASEP SAEFUDDIN Asmarasari, Santiananda Arta Astira, Adinda Wanda B Purwantara Bambang Purwantara Ben J. Takaendengan Ben J. Takaendengan Bramada Winiar Putra Budiariati, Vista Cahyo Budiman Cesilia M Dwiriani Chalid Talib Chalid Talib Clara Meliyanti Kusharto D D Solihin D, Nurhayati Dadan Rohdiana Dairoh Dairoh Dairoh Dairoh, Dairoh Darlian Fachruddin Dedi Duryadi Solihin Dedi Rahmat Depison Depison Depison Depison DEWI APRI ASTUTI Diah Nugrahani Pristihadi Dudi Syadili Dwi Budiono Dwi Margi Suci E Andreas E Taufik E. Andreas E. Andreas E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan Elmy mariana Elmy Mariana Epi Taufik Eryk Andreas Evy Damayanthi Fadhilah Dhani Santika Falah Farajallah, Ahmad Ferdian Achmad Fisma Amri Gilang Kurnia Abdillah Gina, Safira Güler, Serdar Gushairiyanto Gushairiyanto Hadi Riyadi Harahap, Ahmad Saleh HARIMURTI MARTOJO Harini Nurcahya Mariandayani Hasrul Bani Asharudin Heni Rizqiati Heni Rizqiati Henny Nuraini herdis herdis Herpina Herpina Hidayat, Numan I Khaerunnisa I Wayan Mathius I wayan Teguh Wibawan Iman Supriatna Imas Batubara Irma . Irma . Irma Herawati Suparto Irma Irma Irma Isnafia Arief Irmanida Batubara Ishak, Andi Baso Lompengeng Ismeth Inounu Ismeth Inounu Isyana Khaerunnisa Isyana Khaerunnisa Iwan Prihantoro Jakaria Jakaria Jakaria Jakaria Jarmuji Jarmuji Jessy Mainidar Johar Arifin Joni Setiawan Junaedi Junaedi Junaedi Junaedi K Kinoshita Katrin Roosita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komang Alit Paramitasari Komariah . Kuswandi, Wawan Laelatul Choiriyah Lailatul Rohmah Lia Budimulyati Salman Lilis Nuraida Listyarini, Kasita Luci Cyrilla M Imron M S Engel M. Restu Mala Nurilmala Maskur Maskur Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Mochammad Sriduresta Soenarno Mohamad Hasil Tamzil Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin MUHAMMAD AGIL Muhammad Alwi Muhammad Fikri Al Habib Muhammad Suhendra, Muhammad Muhsinin, Muhammad Mutasem Ali M. Abuzahra N Hamashima N Hamashima Nena Hilmia Nensy Tri Putri Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Niswati, Za'imatun Noer Muhammad Dliyaul Haq Noraimah Binti Sulaiman Novita Nur Anggraeni Noviyan Darmawan Nurhayati D Nurhayati, Nurhayati D. Nu’man Hidayat Okti Nadia Poetri Olfa Mega Oloan Parlindungan Pardede, Berlin Pandapotan Pramujo, Muhammad Pratama, Bondan Purwantiningsih Sugita Puspita, Poppy Satya Putri Indah Ningtias R Diyono R Priyanto R. Iis Arifiantini Rahmagiarti, Cintera Rarah Ratih Adjie Maheswari Ratna Sholatia Harahap Ratnawati, Diana Raudah Putri Ekawati Refdanita, Refdanita Restu Misrianti Retno Setyaningsih Rina Martini Rini Herlina Mulyono Rita Mutia Romantis, Sara Rona Saumy Safitry Ronny R. Noor Ronny R. Noor Ronny Rachman Noor Ronny Rahman Noor RR Noor Rudi Afnan Rudi Priyanto Rudi Priyanto Rudy Priyanto Rukmiasih Rukmiasih S Darwati S Kahono S Sopiyana Saiful Anwar Salatnaya, H. Salsabila Ma’shum Imawan Santi Ananda Asmarasari Santiananda Arta Asmarasari Santoso Santoso Sarah Tazkya SATRIYAS ILYAS Septaria Jodiansyah Shidiq, Fajrin Sigid Prabowo Sikin Sikin Simon Elieser Siti Darodjah Siti Zubaidah Slamet Mulyanto Sony Hartono Wijaya Sri Adiani Sri Adiani Sri Darwati Sri Darwati Sri Mulatsih sri murtini . Sri Nurdiati SRI RAHAYU Sri Sulandari Sri Wahyuni Siswanti Sri Wahyuni Siswanti Subandriyo . . Sugyono . Sumiati Sumiati Sutikno Sutikno Sutikno Sutikno Sutikno Sutikno Syah, Setiawan Putra Syamsul Arifin T Akiyama T Morzumi T. Suryati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tatan Kostaman TIKE SARTIKA Tike Sartika Triana Susanti Triana Susanti Tuti Suryati Tuti Suryati Tuty Laswardi Yusuf Ummah, Isna Mustafiatul Vianisa, Putri Wasmen Manalu Weni Kurniati Widiatmaka Widya Pintaka Bayu Putra Wike andre Septian Winni Liani Daulay Yong Soo Kim Yulianto Yulianto Yun Alwi Yureni Sahril Dedi Yusman Setiawan Yusuf, Salman Zakiah Wulandari Zuraida Hanum Zuraida Hanum Zuraida Hanum