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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Acta Aquatica: Aquatic Sciences Journal Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Dinamika Ekonomi - Jurnal Ekonomi dan Bisnis Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Penyuluhan Perikanan dan Kelautan J-ADIMAS (Jurnal Pengabdian kepada Masyarakat) Jurnal Abdi Insani Inspirasi Ekonomi : Jurnal Ekonomi Manajemen Jurnal ABM Mengabdi Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Rahmatan Lil’alamin Journal of Community Services (RLA) Jurnal Pepadu Jurnal Kedokteran Hewan Journal of Fisheries & Marine Journal of Vocational in Aquaculture (JAVA)

WIDANARNI WIDANARNI

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

Author-ID : 415033

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Karakterisasi cairan fermentasi daun mangrove Avicennia marina dan daya hambatnya terhadap bakteri penyebab penyakit ice-ice Samsu Adi Rahman; Sukenda; Widanarni; Alimuddin; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Cairan fermentasi daun mangrove Avicennia marina mengandung mikroorganisme, nutrient, dan metabolit sekunder. Penelitian ini bertujuan untuk mengidentifikasi bakteri dan senyawa dalam cairan fermentasi daun mangrove A. marina dan mengukur daya hambatnya terhadap bakteri patogen Stenotrophomonas maltophilia penyebab penyakit ice-ice pada rumput laut. Hasil analisis molekuler dengan target gen 16S rRNA menunjukkan bahwa bakteri dalam cairan fermentasi terdiri atas delapan jenis Bacillus, yaitu Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, dengan daya hambat terbaik ditunjukkan oleh B. subtilis MSAR-01, B. vietnamensis MSAR-06, dan Bacillus sp. MSAR-07. Pemberian asam laktat, bakteriosin, cairan fermentasi total, dan supernatan sebanyak 15 mL menghasilkan daya hambat terhadap bakteri S. maltophilia lebih baik daripada menggunakan salah satu atau kombinasi beberapa jenis bakteri isolat. Daya hambat cairan fermentasi dan supernatan yang diperkaya bakteri tunggal lebih baik daripada pengayaan kombinasi bakteri. Kata kunci: Avicennia marina, fermentasi, ice-ice, mangrove

Growth performance and immune response of catfish Clarias sp. given probiotics Bacillus megaterium PTB 1.4 and Pediococcus pentosaceus E2211 Muhammad Subhan Hamka; Anja Meryandini; Widanarni
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Motile aeromonad septicaemia (MAS) in catfish can be done by improving the immune responses through probiotic administration. Co-administering probiotics producing digestive enzymes are expected to have an impact on fish growth. This study was aimed to evaluate the effectivity of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 to improve the growth performance and immune response of catfish infected by Aeromonas hydrophila. Catfish with the initial body weight of 7.36 ± 0.21 g were reared in a pond. This study was conducted with five treatments, i. e. K- (without probiotic addition and A. hydrophila injection), K+ (no probiotic addition with A. hydrophila injection), Bm (B. megaterium PTB 1.4 addition and A. hydrophila injection), Pp (P. pentosaceus E2211 addition and A. hydrophila injection), and Bm+Pp (B. megaterium PTB 1.4 addition and P. pentosaceus E2211 and A. hydrophila injection). The study results showed that Bm, Pp, and Bm+Pp treatments were able to improve the growth performance of catfish including digestive enzyme activity, daily growth rate, feed conversion ratio, and final biomass with the best results was Bm+Pp treatment. The immune response of catfish before and after the challenge test showed better survival rate, higher total leukocytes, phagocytic activity, respiratory burst, and lysozyme activity on Bm, Pp, and Bm+Pp treatment (P<0.05) than the control treatment. In conclusion, the combination of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 in feed synergistically improved the growth performance and immune response on catfish against A. hydrophila infection. Keywords: Aeromonas hydrophila, catfish, growth performance, immune response, probiotics ABSTRAK Motile aeromonad septicaemia (MAS) pada ikan lele dapat dikendalikan melalui respons imun inang dengan pemberian probiotik. Pemberian bersama probiotik penghasil enzim pencernaan diharapkan dapat berdampak pada pertumbuhan ikan. Penelitian ini bertujuan mengevaluasi efektivitas pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Ikan lele dengan bobot 7.36 ± 0.21 g dipelihara pada kolam beton yang diberi waring. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K- (tanpa probiotik dan tanpa diinjeksi A. hydrophila), K+ (tanpa probiotik dan diinjeksi A. hydrophila), Bm (diberi B. megaterium PTB 1.4 dan diinjeksi A. hydrophila), Pp (diberi P. pentosaceus E2211 dan diinjeksi A. hydrophila), dan Bm+Pp (diberi B. megaterium PTB 1.4 dan P. pentosaceus E2211 dan diinjeksi A. hydrophila). Hasil penelitian menunjukkan perlakuan Bm, Pp, dan Bm+Pp mampu meningkatkan pertumbuhan ikan lele. Aktivitas enzim pencernaan, laju pertumbuhan harian, rasio konversi pakan, dan biomassa panen dengan hasil terbaik yaitu perlakuan Bm+Pp. Respons imun ikan lele dengan kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 mampu meningkatkan sintasan, total leukosit, aktivitas fagositosis, respiratory burst, dan aktivitas lisozim, baik pada sebelum dan setelah uji tantang. Pemberian kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada pakan mampu bekerja sinergis dalam meningkatkan kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Kata kunci: A. hydrophila, ikan lele, kinerja pertumbuhan, probiotik, respons imun

Reproductive performance of catfish Clarias sp. with probiotics Bacillus sp. NP5 addition through feed Ayuningtyas, Safira Qisthina; Zairin Jr., Muhammad; Widanarni
Jurnal Akuakultur Indonesia Vol. 19 No. 1 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.19.1.74-83

ABSTRACT The intensification of catfish culture requires a regular and sustainable good seeds quality. Microbial-based aquaculture with probiotic application is expected to improve the reproductive performance of catfish. The aim of this study was to evaluate the reproductive performance of catfish given probiotics Bacillus sp. NP5 with different concentrations through feed. This study used a Completely Randomized Block Design (CRBD) with the administration of probiotic Bacillus sp. NP5 at 2% concentration of 108 CFU/mL and 1010 CFU/mL in feed given and control (without probiotics), then repeated four times. Catfish broodstocks with an average size of 955 ± 261.27 g were kept in a net sized 1 m×1 m×1 m with seven broodstocks per net. Parameters observed included the Gonadosomatic Index (GSI), fecundity, number of matured eggs, frequency distribution of egg diameter, fertilization rate, and hatching rate. The result showed that the GSI value of female catfish broodstock was significantly different in sixth week as the highest value was obtained from 1010 CFU/mL probiotics with 5.19%. Moreover, the fecundity of all treatments showed significantly different results (P<0.05) as the highest value was on the concentration of 1010 CFU/mL Bacillus sp. NP5 with 137,123 ± 32,635 eggs/broodstock. This study concludes that the administration of probiotics Bacillus sp. NP5 with 1010 CFU/mL in feed given to catfish broodstock increases the fecundity (137,123 eggs/broodstock), GSI (5.19%), number of matured eggs percentage (64.50%), and hatching rate (82.53%). Keywords: Bacillus sp. NP5, catfish, reproductive performance ABSTRAK Intensifikasi budidaya ikan lele menuntut penyediaan benih yang berkualitas secara teratur dan berkelanjutan. Budidaya berbasis mikroba diharapkan dapat meningkatkan kinerja reproduksi ikan lele. Tujuan penelitian ini adalah mengevaluasi performa reproduksi ikan lele yang diberi probiotik Bacillus sp. NP5 dengan konsentrasi berbeda melalui pakan. Penelitian ini menggunakan rancangan acak kelompok lengkap (RAKL) dengan pemberian probiotik Bacillus sp. NP5 pada konsentrasi 108 CFU/mL dan 1010 CFU/mL sebanyak 2% dari pakan yang diberikan serta kontrol (tanpa pemberian probiotik), masing-masing dengan empat ulangan. Induk ikan lele dengan ukuran 955 ± 261.27 g dipelihara pada jaring hapa ukuran 1 m × 1 m × 1 m sebanyak tujuh ekor per jaring hapa. Parameter yang diamati meliputi indeks kematangan gonad (IKG), fekunditas, jumlah telur matang, sebaran frekuensi diameter telur, derajat pembuahan, dan derajat penetasan. Hasil penelitian menunjukkan bahwa nilai IKG induk betina ikan lele berbeda nyata pada minggu keenam dengan nilai tertinggi diperoleh pada perlakuan probiotik 1010 CFU/mL yaitu sebesar 5.19%. Selain itu, fekunditas semua perlakuan menunjukkan hasil yang berbeda nyata (P<0.05) dengan nilai tertinggi pada perlakuan pemberian probiotik Bacillus sp. NP5 dengan konsentrasi 1010 CFU/mL sebesar 137,123 ± 32,635 telur/induk. Kesimpulan, pemberian probiotik Bacillus sp. NP5 dengan konsentrasi 1010 CFU/mL melalui pakan kepada induk ikan lele mampu meningkatkan nilai fekunditas 137,123 telur/induk, GSI 5,19%, persentase jumlah telur matang 64,50% dan derajat penetasan telur 82,53%. Kata kunci: Bacillus sp., ikan lele, performa reproduksi

Growth performance, immune response, and resistance of Nile tilapia fed paraprobiotic Bacillus sp. NP5 against Streptococcus agalactiae infection Aldy Mulyadin; Widanarni, Widanarni; Yuhana, Munti; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 20 No. 1 (2021): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.20.1.34-46

ABSTRACT This study was aimed to evaluate the effectiveness of Bacillus sp. NP5 paraprobiotic administration through commercial feed on growth performance, immune response, and resistance of Nile tilapia against Streptococcus agalactiae infection. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h, then performed a viability test on tryptic soy agar (TSA) media and incubated for 24 hours. Paraprobiotics could be used whether the bacteria did not grow on the TSA media. This study used a completely randomized design, containing three treatments with five replications, i.e. 1% (v/w) probiotic addition, 1% (v/w) paraprobiotic addition, and no addition of probiotic or paraprobiotic (control). The experimental fish were reared for 30 days. On day 31 of rearing, fish were challenged with S. agalactiae (107 CFU/mL) through intraperitoneal injection route, while the negative control was injected with PBS. This study results significantly improved growth performances and immune responses (P<0.05), compared to control after 30 days of probiotic and paraprobiotic Bacillus sp. NP5 administration. After challenge test, increased immune responses in probiotic and paraprobiotic of Bacillus sp. NP5 treatment had higher survival rates (P<0.05) than positive control. The administration of Bacillus sp. NP5 probiotic and paraprobiotic through commercial feed were effective in increasing growth performance, immune response, and resistance of Nile tilapia against S. agalactiae infection. Keywords: Bacillus sp. NP5, Nile tilapia, paraprobiotic, Streptococcus agalactiae ABSTRAK Penelitian ini bertujuan mengevaluasi efektivitas pemberian paraprobiotik Bacillus sp. NP5 melalui pakan dalam meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi Streptococcus agalactiae. Proses pembuatan bakteri paraprobiotik yaitu Bacillus sp. NP5 diinaktivasi panas pada suhu 95°C selama 1 jam, dilanjutkan dengan pengujian viabilitas dengan menyebarkannya pada media tryptic soy agar kemudian diinkubasi selama 24 jam. Jika bakteri tidak tumbuh, maka paraprobiotik dapat digunakan. Penelitian ini menggunakan rancangan acak lengkap dengan tiga perlakuan dan lima ulangan, yaitu penambahan probiotik 1% (v/w), penambahan paraprobiotik 1% (v/w), dan tanpa penambahan probiotik atau paraprobiotik (kontrol). Ikan perlakuan dipelihara selama 30 hari dan pada hari ke-31, ikan diuji tantang dengan S. agalactiae (107 CFU/mL) melalui injeksi intraperitoneal, sementara perlakuan kontrol negatif diinjeksi dengan PBS. Hasil penelitian setelah 30 hari pemberian probiotik dan paraprobiotik Bacillus sp. NP5 menunjukkan kinerja pertumbuhan dan respons imun yang meningkat signifikan (P<0.05) dibandingkan dengan kontrol. Pascauji tantang, peningkatan respons imun pada perlakuan probiotik dan paraprobiotik Bacillus sp. NP5 menunjukkan tingkat kelangsungan hidup yang lebih tinggi (P<0.05) dibandingkan kontrol positif. Pemberian probiotik dan paraprobiotik Bacillus sp. NP5 melalui pakan dapat meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi Streptococcus agalactiae. Kata kunci: Bacillus sp. NP5, ikan nila, paraprobiotik, Streptococcus agalactiae

Controlling the cannibalism of African catfish juvenile by 17β‒estradiol hormone administration and the stocking density determination Siregar, Khoirotun Nisa; Maulana, Fajar; Zairin Jr., Muhammad; Alimuddin, Alimuddin; Widanarni, Widanarni
Jurnal Akuakultur Indonesia Vol. 20 No. 1 (2021): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.20.1.72-81

An effort to increase the production of juvenile catfish is limited by the high mortality rate, especially caused by cannibalism. The hormonal treatment has been conducted as an effort to control cannibalism. This study used completely randomized factorial design, consisted of six treatments and three replications. There were two factors examined in this study, the first factor was different doses of 17β‒estradiol hormone (0, 30, and 60 mg estradiol‒17β/kg) incorporated in the feed, and the second factor was stocking density (150 and 300 fish/m2). Juvenile catfish with the size of 4.0 ± 0.1 cm were reared for 30 days in the 84 L aquarium. The results showed that hormone treatment could reduce cannibalism rate, type-I and II cannibalism compared to control (P<0.05). The results showed that the lowest of mortality was obtained in the treatment B (17β‒estradiol administration of 30 mg/kg, at stocking density of 150 fish/m2; P<0.05). The lowest cortisol level was found in treatment F, and the highest estradiol level was found in treatment F (17β‒estradiol administration of 60 mg/kg, at stocking density of 300 fish/m2; P<0.05). The lowest of blood glucose level was found in treatment B (P<0.05). The highest specific weight growth was found in the stocking density of 150 fish/m2 (P<0.05). The results of this study indicated that administration of 17β‒estradiol in feed could reduce the level of cannibalism in African catfish juvenile. Keywords: cannibalism, Juvenile, catfish, estradiol‒17β, cortisol ABSTRAK Upaya untuk meningkatkan produksi benih ikan lele dibatasi oleh tingginya angka kematian, terutama yang disebabkan oleh kanibalisme. Pendekatan hormonal telah dilakukan sebagai upaya pengendalian kanibalisme. Penelitian ini menggunakan rancangan acak lengkap faktorial, terdiri dari enam perlakuan dan tiga ulangan. Ada dua faktor yang diteliti dalam penelitian ini, faktor pertama adalah dosis hormon 17β‒estradiol yang berbeda (0, 30, dan 60 mg/kg) yang diberikan melalui pakan, dan faktor kedua adalah padat tebar (150 dan 300 ekor/m2). Benih ikan lele berukuran 4.0 ± 0.1 cm dipelihara selama 30 hari di akuarium (84 L). Hasil penelitian menunjukkan bahwa perlakuan hormon dapat menurunkan tingkat kanibalisme tipe‒I dan II dibandingkan kontrol (P<0.05). Hasil penelitian menunjukkan bahwa mortalitas terendah diperoleh pada perlakuan B (pemberian 17β‒estradiol 30 mg/kg, pada padat tebar 150 ekor/m2; P<0.05). Kadar kortisol terendah ditemukan pada perlakuan F, dan kadar estradiol tertinggi ditemukan pada perlakuan F (pemberian 17β‒estradiol 60 mg/kg, pada padat tebar 300 ekor/m2; P<0.05). Kadar glukosa darah terendah ditemukan pada perlakuan B (P<0.05). Pertumbuhan bobot spesifik tertinggi ditemukan pada padat tebar 150 ekor/m2 (P<0.05). Hasil penelitian ini menunjukkan bahwa pemberian hormon 17β‒estradiol dengan dosis 30 mg/kg pakan, dan padat tebar 150 ekor/m2 dapat menurunkan tingkat kanibalisme pada benih ikan lele di Afrika. Kata kunci: kanibalisme, juvenil, lele, 17β‒estradiol, kortisol

Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment Munti Yuhana; Tambun, Andreas; Widanarni, Widanarni; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.125-132

This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.

The effects of different dosage applications of Bacillus sp. NP5 para-probiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infection Widanarni; Sukenda; Annisa Astri Anggraeni; Rr. Bellya Anasti Maharani; Aldy Mulyadin; Munti Yuhana
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.186-197

The aim of the study is to test the effectiveness of adding various paraprobiotic doses of Bacillus sp. NP5 through feed in improving the immune responses and resistance of tilapia juvenile (Oreochromis niloticus) to infection of Streptococcus agalactiae. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h. This study used a completely randomized design, which consisted of four treatments with three repetitions. The test feed was enriched with 1% paraprobiotic with a density of 108, 109, and 1010 CFU ml-1. The tilapia juvenile (10.29 ± 0.22 g) were reared for 30 days and on day 31, the fish was challenge tested with S. agalactiae (107 CFU ml-1) injected by intraperitoneal route unless the negative control was injected with PBS. The results of this study showed significantly improved growth performances and immune responses (P<0.05), compared to the control group after 30 days of paraprobiotic Bacillus sp. NP5 administered. The increased of immune responses in paraprobiotic of Bacillus sp. NP5 showed a higher survival rate (P<0.05), than positive control after the challenge tested with S. agalactiae. Administration of paraprobiotic Bacillus sp. NP5 through the commercial feed able to improve the growth performances, immune responses, and resistance of nile tilapia against S. agalactiae infection with the best result at dose of 1010 CFU ml-1.

The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus Ode, Inem; Sukenda; Widanarni; Dinamella Wahjuningrum; Munti Yuhana; Mia Setiawati
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.1-11

Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper infected with V. alginolyticus. The clove extraction used a maceration method. Two dose levels of clove powder were used, namely 10 and 15 g kg−1. The control treatments without clove powder supplementation contained positive control (CP) and negative control treatment (CN). The results found that the clove extract contained five major compounds. The highest compound was phenol, 2-methoxy-4-(2-propenyl)-Eugenol (64.07%). Phytochemical analysis of clove extract contained phenolics, flavonoids, and tannins at (28.53 ± 0.00) mg/g, (0.38 ± 0.00) mg/g, and (0.15 ± 0.00) %, respectively. The diameter of the clove extract inhibition zone was significantly different (P < 0.05) in all treatments. The Scanning Electron Microscopy (SEM) result presents that the clove extract can alter the V. alginolyticus cell morphology. The dietary supplementation of clove powder improves the survival rate significantly higher (P<0.05) post-challenge test. The conclusion of this research is that clove extract has antibacterial activity that can inhibit growth and cause cell morphological damage to V. alginolyticus. The application of clove powder at a dose of 15g kg-1 was able to improve the survival value which was a higher post-challenge test. Keyword: antibacterial activity, clove, grouper, phytochemical, Vibrio alginolyticus

The health status of hybrid grouper Epinephelus fuscoguttatus (♀) x E.lanceolatus (♂) cultured in floating-net cage at Kelapa Dua Island, Seribu Islands, Indonesia Ratu Borut, Ruku; Sukenda; Sri Nuryati; kukuh Nirmala; Irzal Effendi; Widanarni
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.106-114

This study aimed to identify the fish health status, based on the total erythrocytes (cells/mm3), total leukocytes (cells/mm3), hemoglobin level (g %), hematocrit, and histopathological profiles. This study was performed on February, 2021 – January, 2022. The study was located in floating-net cage of Kelapa Dua Island, Seribu Islands, Jakarta, Indonesia. Sampling was performed by a purposive-sampling method, environmental parameter measurements, containing temperature and salinity, clinical symptom observation in fish, total erythrocytes, leukocytes, hemoglobin, hematocrit, histopathological profiles of hybrid grouper liver and kidney, and existence of VNN virus. The observation results present the behavior changes in sick fish, namely whirling on the net cage surface, dead-sleeping or death-like condition below the net cage. Hybrid grouper has total erythrocytes of 1.36 x 106 – 5.13 x 106 cells/mm3, total leukocytes of 2.9 x 104 – 7.9 x 104 cells/mm3, hemoglobin level of 5.56 - 10.94 g %, and hematocrit of 13.31 - 24.78 %. From the histopathological observation, hepatocyte, necrosis, vacuolysis was occurred in liver and tubular, leukocyte infiltasi, nicrosis was occurred in kidney and with the highest prevalence of VNN at 20 – 80 %. Therefore, hybrid grouper is sick due to increased number of leukocytes at 5.13 x 106 cells/mm3 above the normal range, followed by decreased hemoglobin and hematocrit levels at 5.56 - 10.94 g % and 13.31 - 24.78 %, respectively, due to organ damage. Keyword: must be written in not more than 5 words and alphabeticaly listed ABSTRAK Penelitian ini bertujuan untuk mengetahui status kesehatan ikan meliputi total eritrosit (sel/mm3), total leukosit (sel/mm3), kadar hemoglobin (gram %) dan hematokrit (%) dan gambaran histopatolgi. Penelitian ini dilaksanakan pada periode bulan Februari 2021 hingga Januari 2022. Lokasi penelitian pada keramba jaring apung (KJA) di Pulau Kelapa Dua Kepulauan Seribu. Metode pengambilan sampel ikan dilakukan secara purposive sampling, pengukuran parameter lingkungan perairan yaitu suhu dan salinitas, pengamatan gajala klinis ikan, perhitungan jumlah eritrosit, leukosit, kadar hemoglobin dan hematokrit serta gambaran histopatologi pada organ hati dan ginjal ikan kerapu cantang dan keberadaan virus VNN. Hasil pengamatan menunjukkan perubahan tingkah laku ikan sakit yaitu berputar ke atas permukaan KJA, terjadi sleeping dead atau ikan berada di dasar seperti mati. Ikan kerapu cantang memiliki jumlah eritrosit 1.36 x 106 – 5.13 x 106 sel/mm3, leukosit 2.9 x 104 – 7.9 x 104 sel/mm3, hemoglobin 5.56 - 10.94 gr %, dan hematokrit 13.31 - 24.78 %, hasil histopalogi ikan mengalami perubahan sel pada hati yaitu hepatosit, vakuolisasi, nekrosis dan ginjal mengalami perubahan infiltrasi leukosit, nicrosis, tubulus dan kehadiran virus VNN tertinggi berkisar 20 % - 80 %. Berdasarkan hasil penelitian tersebut maka dapat disimpulkan bahwa ikan kerapu cantang yang sakit karena jumlah leukosit meningkat 5.13 x 106 sel/mm3 melebihi kisaran normal, kadar hemoglobin menurun 5.56 - 10.94 % dan kadar hematokrit 13.31 - 24.78 % rendah dan terjadi kerusakan jaringan pada organ dalam ikan. Kata kunci: Ikan Kerapu Cantang, Hematology, Histopatologi, Parameter Fisika dan Kimia

Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp. Sudrajat, R Herman; Yuhana, Munti; Widanarni, Widanarni; Julie Ekasari; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.134-146

Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P<0.05) higher probiotic stimulation value than other doses. In the in vivo test, the study used a completely randomized design with five treatments, namely simplicia (DOSP 20 g/kg), probiotic (PRO, Bacillus sp. NP5 108 CFU/mL, 1% v/w), combination (PRO+DOSP), and control (positive and negative). Fish were reared for 45 days and fed three times a day. On day 46, fish from all treatments, except negative control, were infected with an A. hydrophila dose of 106 CFU/mL injected intramuscularly. The results showed that the combination treatment (PRO+DOSP) gave better total erythrocytes, hemoglobin, hematocrit, total leukocytes and phagocytosis activity than probiotics, DOSP, and control. Administering the combination (PRO+DOSP) can reduce the total number of A. hydrophila lower than the probiotic, DOSP, and control treatments. In addition, the survival rate of catfish in the combined treatment (PRO+DOSP) was significantly (P<0.05) higher than probiotics, DOSP, and control. The results of this study can be a helpful reference and application for the early prevention of A. hydrophila infection. Keywords: aquaculture, Bacillus sp. NP5, dayak onion, probiotics, simplicia powder ABSTRAK Aeromonas hydrophila adalah penyebab utama penyakit bercak merah pada budidaya ikan lele dan menyebabkan kerugian ekonomi cukup besar pada akuakultur Indonesia. Penelitian ini bertujuan untuk mengevaluasi aktivitas prebiotik serbuk simplisia bawang dayak (SSBD) dan pengaruh penambahan kombinasi probiotik Bacillus sp. NP5 dan SSBD pada pakan terhadap respons imun dan kelangsungan hidup ikan lele yang diinfeksi A. hydrophila. Lima dosis (0,20, 0,25, 0,30, 0,35, dan 0,40 g/mL) SSBD diuji secara in vitro untuk menilai skor aktivitas prebiotik. Hasil penelitian menunjukkan bahwa dosis 0,20 g/mL memberikan nilai stimulasi probiotik signifikan (P<0,05) lebih tinggi dibandingkan dosis lainnya. Pada uji in vivo, penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan, yaitu simplisia (SSBD, serbuk simplisia bawang dayak 20 g/kg), probiotik (PRO, Bacillus sp. NP5 108 CFU/mL, 1% (v/w), kombinasi (PRO+SSBD), dan kontrol (positif dan negatif). Ikan dipelihara selama 45 hari dan diberi pakan tiga kali sehari. Pada hari ke 46, ikan pada semua perlakuan, kecuali kontrol negatif, di infeksi A. hydrophila dosis 106 CFU/mL secara intramuskular. Hasil penelitian menunjukkan bahwa kombinasi (PRO+SSBD) memberikan total eritrosit, hemoglobin, hematokrit, total leukosit dan aktivitas fagositosis lebih baik dibandingkan probitoik, SSBD, dan kontrol. Kombinasi (PRO+SSBD) mampu menekan total A. hydrophila lebih rendah dibandingkan probiotik, SSBD, dan kontrol. Selain itu, tingkat kelangsungan hidup ikan lele pada perlakuan kombinasi (PRO+SSBD) signifikan (P<0,05) lebih tinggi dibandingkan probitoik, SSBD, dan kontrol. Hasil penelitian ini bisa menjadi referensi dan aplikasi yang efektif untuk pencegahan dini infeksi A. hydrophila. Kata kunci: akuakultur, Bacillus sp. NP5, bawang dayak, probiotik, serbuk simplisia

Co-Authors . Sunarto A. Sunarma A. SUWANTO A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Anjarweni, Vika Atinia Annisa Astri Anggraeni Antonius Suwanto Apriani, Ita Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika B. W. LAY Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fahmi Rajab Fauzi, Arini Resti Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Marli, Marli Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasri Julaini Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Reza Samsudin Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rizkiyanti, Ita Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Saputri, Rika Ani Septiani, Ghita Ryan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . SUKENDA SUKENDA Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suprianto, Dedi Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Taufiq Abdullah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wicaksono, Baref Agung Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad

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