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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Acta Aquatica: Aquatic Sciences Journal Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Dinamika Ekonomi - Jurnal Ekonomi dan Bisnis Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Penyuluhan Perikanan dan Kelautan J-ADIMAS (Jurnal Pengabdian kepada Masyarakat) Jurnal Abdi Insani Inspirasi Ekonomi : Jurnal Ekonomi Manajemen Jurnal ABM Mengabdi Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Rahmatan Lil’alamin Journal of Community Services (RLA) Jurnal Pepadu Jurnal Kedokteran Hewan Journal of Fisheries & Marine Journal of Vocational in Aquaculture (JAVA)

WIDANARNI WIDANARNI

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

Author-ID : 415033

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Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus Ilmiah, .; Sukenda, .; Widanarni, .; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus. Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper

Oral application of probiotic, prebiotic, and synbiotic in Pacific white shrimp(Litopenaeus vannamei)challenged with Vibrio harveyi Widanarni, .; Widagdo, Puguh; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of antibiotics for controlling of luminous vibriosis caused by Vibrio harveyi is restricted now, because it induces antibiotic-resistant bacteria and leave residue in shrimp’s body. An alternative solution that can be done to treat the disease is by using applications of probiotic, prebiotic, and synbiotic. The aim of this research was to examine the effect of probiotic, prebiotic, and synbiotic on the survival rate and growth of Pacific white shrimp against V. harveyi infection. Feed as a treatment was supplemented with probiotic 1%, prebiotic 2%, and probiotic 1%+prebiotic 2% (synbiotic). Shrimps feed without supplementation of probiotic and prebiotic was used as a control treatment. The shrimps were maintainedin the aquarium (60×30×35 cm3) with a density of 40 shrimps/40 L and an average weight of 0.4±0.1 g. After 30 days of feeding treatment, the shrimp was challenged by immersion method with V. harveyi solution containing 106 CFU/mL. The results showed that before challenge, synbiotic feed treated shrimp has a growth rate (5.89%), feed conversionrate (1.21), and a high survival rate (80%). After challenge, survival rate (83.33%) of shrimp fed diet supplemented with synbiotic was higher than prebiotic (51.67%) and positive control (31.67%). Keywords: probiotic, prebiotic, synbiotic, Vibrio harveyi,Pacific white shrimp

Selection of probiotic bacteria from the coral reef and tiger grouper fish (Epinephelus foscoguttatus) farming environment Ilmiah, .; Sukenda, .; Widanarni, .; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of probiotic bacteria is one of the methods in aquaculture to control infectious diseases. This study aims to obtain candidates probiotic bacteria from the coral reef and tiger grouper aquaculture environment (Epinephelus fuscoguttatus). Steps of the study included of: 1) isolation of candidates probiotic bacteria, 2) selection of candidates probiotic bacteria: inhibition test and co-culture methods, 3) enzymatic test, 4) the growth of bacteria test 5) pathogenicity test and 6) the molecular identification of probiotic bacteria. The results showed that of 124 isolates in test zone of inhibition, the four isolates with the widest zone of inhibition of isolate K7, K8, K21, and T4, a total of 51 isolates on co-culture methods, the four isolates were able to suppress the growth of pathogenic Vibrio bacteria, isolates K15, K68, K55, and T36. The results of hydrolyzing the highest protease amylase and lipase enzyme, respectively isolates K15 and T36; isolates T41 and K8; isolates K55 and K68. Candidates probiotic bacteria reached the exponential phase at 16‒18 h. The results of pathogenicity test isolate K7, K8, T36, and T41 provided the highest survival that can be used as candidates probiotics in the tiger grouper. Keywords: selection, probiotics, tiger grouper

Growth of tiger shrimp Penaeus monodon post-larvae fed on Artemia containing Vibrio SKT-b probiotic Widanarni, .; Hadiroseyani, Yani; Sutanti, Asri
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Application of probiotic bacteria is an alternative technology to increase shrimp production in an environmentally friendly aquaculture. Administration of probiotic bacteria can be conducted through artificial feed or live food such as Artemia. This study was done to examine the effectiveness of various doses of probiotic Vibrio SKT-b through Artemia on the growth and survival of post-larval shrimp. Tiger shrimp at a stage of PL 10 was reared in glass jars filled with 2 L of sea water at a density of 10 larva/L. The study consisted of five probiotic concentrations control (0 cfu/mL), A (103 cfu/mL), B (104 cfu/mL), C (105 cfu/mL), and D (106 cfu/mL). Administration of various doses of probiotic bacteria Vibrio SKT-b through Artemia significantly increased the growth rate in term of the length and weight, but had no effect on survival. The results found that treatment D (106 cfu/mL) gave an increase in body weight, length and survival rate of 22.53%/day, 0.080 cm/day and 95%, respectively. Keywords: probiotic, Artemia, tiger shrimp ABSTRAK Aplikasi bakteri probiotik merupakan salah satu alternatif teknologi untuk meningkatkan produksi budidaya udang yang ramah lingkungan. Pemberian bakteri probiotik dapat dilakukan melalui pakan buatan atau pakan alami seperti Artemia. Penelitian ini bertujuan untuk menguji efektivitas pemberian berbagai dosis bakteri probiotik Vibrio SKT-b melalui Artemia terhadap pertumbuhan dan kelangsungan hidup pascalarva udang windu. Stadia udang windu yang digunakan adalah pascalarva (PL) 10. Udang dipelihara dalam wadah kaca volume 3 L yang diisi air laut 2 L dengan kepadatan 10 ekor/L. Penelitian ini terdiri atas lima perlakuan yaitu kontrol (dosis bakteri probiotik 0 cfu/mL), A (103 cfu/mL), B (104 cfu/mL), C (105 cfu/mL), dan D (106 cfu/mL). Hasil penelitian menunjukkan bahwa pemberian berbagai dosis bakteri probiotik Vibrio SKT-b melalui Artemia berpengaruh nyata terhadap laju pertumbuhan panjang dan bobot, namun tidak berpengaruh terhadap nilai kelangsungan hidup. Hasil terbaik diperoleh pada perlakuan D (106 cfu/mL) dengan pertumbuhan bobot 22,53%/hari, pertumbuhan panjang 0,08 cm/hari, dan sintasan 95%. Kata kunci: probiotik, Artemia, udang windu

Effect of molases addition on survival and growth of tiger shrimp Penaeus monodon Fab. larva treated with SKT-b Vibrio probiotic bacteria Widanarni, ,; Saputra, Wira H; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTProbiotic bacteria SKT-b Vibrio that belong to heterotrophic bacteria have been tested can suppress the growth of pathogenic Vibrio harveyi in tiger shrimp larvae. One of the most important energy sources of heterotrophic bacteria is organic carbon such as sucrose and glucose found in molasses. The objective of this experiment was to study the effect of molasses addition on the survival and growth rate of tiger shrimp Penaeus monodon larvae treated with SKT-b Vibrio probiotic bacteria. This experiment was done both in vitro and in vivo. In vitro tests performed by growing bacteria in media containing molasses, fish meal and premix. Whereas in vivo tests performed with the addition of molasses to shrimp larva culture media inoculated with SKT-b Vibrio bacteria which included five treatments: the control treatment without bacteria and molasses addition (K), the addition of bacteria without molasses (B), the addition of molasses 1 ppm and bacteria (MB1), the addition of molasses 3 ppm and bacteria (MB3), and the addition of molasses 5 ppm and bacteria (MB5). The result of in vitro tests showed the SKT-b Vibrio bacteria can grow on molasses media with a population reached 0,41 × 108 CFU/ml. In vivo test results showed that treatment MB3 gave the highest survival (93.3%) and growth weight (35.94%), but were not significantly different from control result in the survival and growth weight that respectively were 83.3% and 30.38%.Keywords: probiotic bacteria, SKT-b Vibrio, molasses, tiger shrimp larvae ABSTRAKBakteri probiotik Vibrio SKT-b yang tergolong bakteri heterotrof telah diuji dapat menekan pertumbuhan Vibrio harveyi yang bersifat patogen pada larva udang windu. Salah satu sumber energi yang paling penting bagi bakteri heterotrof adalah karbon organik seperti sukrosa dan glukosa yang terdapat pada molase. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan molase terhadap kelangsungan hidup dan pertumbuhan larva udang windu Penaeus monodon Fab. yang diberi bakteri probiotik Vibrio SKT-b. Penelitian dilakukan secara in vitro dan in vivo. In vitro yaitu menumbuhkan bakteri pada media yang mengandung molase, tepung ikan dan premix. In vivo yaitu penambahan molase pada media pemeliharaan udang yang diberi bakteri probiotik Vibrio SKT-b, yang meliputi lima perlakuan yakni: perlakuan kontrol tanpa penambahan bakteri maupun molase (K), penambahan bakteri tanpa molase (B), penambahan molase 1 ppm dan bakteri (MB1), penambahan molase 3 ppm dan bakteri (MB3), penambahan molase 5 ppm dan bakteri (MB5). Hasil uji in vitro menunjukkan bakteri Vibrio SKT-b dapat tumbuh pada media molase dengan populasi mencapai 0,41×108 CFU/mL. Hasil uji in vivo menunjukkan perlakuan MB3 menghasilkan kelangsungan hidup (93,3%) dan pertumbuhan bobot (35,94%) tertinggi, namun tidak berbeda nyata dengan kontrol yang menghasilkan kelangsungan hidup dan pertumbuhan bobot berturut-turut 83,3% dan 30,38%.Kata kunci: bakteri probiotik, Vibrio SKT-b, molase, larva udang windu

Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; ., Widanarni; ., Sukenda
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang

Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene Widanarni, ,; Nurhayati, Dewi; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. A total of 38 dominant bacterial isolates was obtained from bioflocs samples and of these isolates, 16S-rRNA gene was then isolated and amplified using PCR. The 16S-rRNA gene of the isolates was then cut using HaeIII (5’-GG↓CC) and HhaI (5’-GCG↓C) restriction enzymes resulting an ARDRA pattern which was further used as the binary data for the construction of phylogenetics tree that was used to estimate the group of bacteria. The result with HaeIII cut restriction enzyme from biofloc-associated bacteria gave 11 ARDRA patterns, while with the restriction enzyme HhaI gave eight ARDRA patterns. Phylogenetics of bacterial populations from biofloc-based cultivation system water consisted of at least 13 different bacterial species. Result of sequencing from two gene sample 16S-rRNA were identified as Microbacterium foliorumand and Pseudomonas putida. Keywords: bacterial diversity, ARDRA, biofloc, phylogeny ABSTRAK Penelitian ini bertujuan untuk menganalisis keragaman genetika bakteri bioflok menggunakan metode polymerase chain reaction (PCR) 16S-rRNA dengan teknik ARDRA. Sebanyak 38 isolat bakteri dominan yang diperoleh diamplifikasi gen 16S-rRNAnya dengan PCR, kemudian dipotong dengan enzim restriksi HaeIII (5’-GG↓CC) dan HhaI (5’-GCG↓C). Pola ARDRA ini dijadikan data biner sebagai input untuk konstruksi pohon filogenetika yang dapat digunakan untuk memerkirakan jenis bakteri yang ada. Gen 16S-rRNA hasil PCR setelah dipotong dengan enzim restriksi HaeIII didapatkan 11 pola ARDRA, sedangkan dengan enzim restriksi HhaI menghasilkan delapan pola ARDRA. Berdasarkan pohon filogenetika, diketahui populasi bakteri pada air sistem budidaya bioflok sedikitnya terdiri atas 13 jenis bakteri. Berdasarkan sekuensing dari dua sampel gen 16S-rRNA teridentifikasi jenis bakteri Microbacterium foliorum dan Pseudomonas putida. Kata kunci: keragaman bakteri, ARDRA, bioflok, filogenetika

Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper Saputra, Dwi Agung; Sukenda, ,; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment feeds with supplementation of synbiotic with different doses of probiotic (probiotic 104 cfu/mL) 1% + prebiotic 2% (P1) v/w, (probiotic 106 cfu/mL) 1% + prebiotic 2% (P2) v/w, (probiotic 108 cfu/mL)1% + prebiotic 2% (P3) v/w) for 30 days. After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. Then all the grouper were challenged by Vibrio alginolyticus, except K (-). This study showed that survival, daily growth and food conversion ratio (FCR) of grouper in treatment P2 ((probiotic 106 cfu/mL) 1% + prebiotic 2%) and P3 (probiotic (108 cfu/mL) 1% + prebiotic 2%) were significantly better (P>0.05) than controls. The different doses of probiotic in synbiotic (probiotic 104 cfu/mL, probiotic 106 cfu/mL and probiotic 108 cfu/mL) provided better immune response than controls. Keywords: synbiotic, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis ABSTRAK Tujuan penelitian ini adalah mengkaji pemberian sinbiotik dengan dosis probiotik berbeda untuk mencegah penyakit vibriosis pada ikan kerapu bebek (Cromileptes altivelis). Ikan kerapu dengan berat (3,0±0,48 g) diberikan pakan kontrol (tanpa penambahan sinbiotik) K(-) dan K(+), pakan perlakuan dengan penambahan sinbiotik dengan dosis probiotik berbeda: probiotik ((104 cfu/mL) 1% + prebiotik 2%) v/w (P1), ((probiotik 106 cfu/mL) 1% + prebiotik 2%) v/w (P2), dan ((probiotik 108 cfu/mL)1% + prebiotik 2%) v/w (P3) selama 30 hari. Setelah perlakuan pakan sinbiotik, dilakukan pengamatan terhadap jumlah total bakteri di usus, kinerja pertumbuhan dan respons imun. Kemudian ikan kerapu pada seluruh perlakuan, kecuali kontrol negatif (-) diberi uji tantang dengan Vibrio alginolyticus. Hasil penelitian menunjukkan bahwa sintasan, laju pertumbuhan harian (LPH), dan rasio konversi pakan (FCR) pada perlakuan P2 ((probiotik 106 cfu/mL) 1% + prebiotik 2%) dan P3 (probiotik (108 cfu/mL) 1% + prebiotik 2%) secara signifikan lebih baik (P<0,05) bila dibandingkan dengan kontrol. Perlakuan sinbiotik dengan dosis probiotik berbeda (probiotik 104 cfu/mL, probiotik 106 cfu/mL, dan probiotik 108 cfu/mL) juga memberikan respons imun yang lebih baik dibandingkan dengan kontrol. Kata kunci : sinbiotik, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis

Prebiotic, probiotic, and synbiotic to control Vibrio harveyi and IMNV co-infection in Litopenaeus vannamei Widanarni, ,; Noermala, Jeanni Indah; Sukenda, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to examine the effects of prebiotic, probiotic, and synbiotic in survival and immune response of white shrimp against co-infection of Vibrio harveyi and infectious myonecrosis virus (IMNV). The shrimps used had a body weigth of 2.04±0.20 g/individual, the shrimps were reared at a density of 20 shrimps in 60x30x35 cm3 sized aquarium. The study was conducted with five treatments consisting K(+) (without the addition of prebiotic, probiotic, and synbiotic with co-infection), K(-) (without the addition of prebiotic, probiotic and synbiotic, and without co-infection), P1 (the addition of prebiotic with co-infection), P2 (the addition of probiotic with co-infection), and P3 (the addition of synbiotic with co-infection). The results showed that the addition of prebiotic, probiotic and synbiotic could increase survival and immune response of white shrimp towards co-infection of Vibrio harveyi and IMNV. The best survival was obtained in probiotic treatment (79.17%), followed by prebiotic treatment (75%), synbiotic treatment (70.83%), while the positive control was only 50%. Keywords: white shrimp, prebiotic, probiotic, synbiotic, IMNV, Vibrio harveyi ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh pemberian prebiotik, probiotik, dan sinbiotik terhadap sintasan dan respons imun udang vaname dengan ko-infeksi Vibrio harveyi dan IMNV (infectious myonecrosis virus). Udang yang digunakan memiliki bobot 2,04±0,20 g/ekor, dipelihara sebanyak 20 ekor dalam akuarium berukuran 60x30x35 cm3. Penelitian dilakukan dengan lima perlakuan yaitu K(+) (tanpa pemberian prebiotik, probiotik, dan sinbiotik dengan ko-infeksi), K(-) (tanpa pemberian prebiotik, probiotik dan sinbiotik tanpa ko-infeksi), P1 (pemberian prebiotik dengan ko-infeksi), P2 (pemberian probiotik dengan ko-infeksi), dan P3 (pemberian sinbiotik dengan ko-infeksi). Hasil penelitian menunjukkan bahwa pemberian prebiotik, probiotik, dan sinbiotik mampu meningkatkan sintasan dan respons imun udang vaname terhadap ko-infeksi V. harveyi dan IMNV. Sintasan terbaik diperoleh pada perlakuan probiotik sebesar 79,17%, diikuti perlakuan prebiotik sebesar 75%, perlakuan sinbiotik sebesar 70,83%, sedangkan pada kontrol positif hanya mencapai 50%. Kata kunci: udang vaname, prebiotik, probiotik, sinbiotik, IMNV, Vibrio harveyi

Application of Bacillus probiotic to prevent Aeromonas hydrophilla infection in Clarias sp. Ulkhaq, Mohammad Faizal; Widanarni, ,; Kusumastuti, Angela Mariana
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to test the effectiveness of a probiotic Bacillus for the prevention of motile aeromonad septicemia (MAS) disease caused by Aeromonas hydrophila in African catfish (Clarias gariepinus). The study consisted of the inhibition testing of A. hydrophila by Bacillus (in vitro) and the application of probiotic in African catfish (in vivo). The in vivo test, consisted of five treatments such as the addition of probiotic Bacillus P4I1 RifR, Bacillus P4I2 RifR, Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), positive control (K+; only added with A. hydrophila) and negative control (K-; without probiotic nor A. hydrophila addition). African catfish (13.35±2.80 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila RifR (103 cfu/mL) were added once in earlier treatment (except for the negative control). The result showed that the optimal concentration of Bacillus to inhibit A. hydrophila on in vitro test was 104 cfu/mL. In vivo test showed that the addition of probiotic in media of cultivation could reduce the number of A. hydrophila, improve immune response, and also increase the survival of African catfish compared to positive control. Application of probiotic P4I1 RifR showed the highest survival (92.23%) of all treatments. Keywords: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotic ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas probiotik Bacillus dalam pencegahan penyakit motile aeromonad septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan lele dumbo (Clarias gariepinus). Penelitian terdiri atas pengujian penghambatan bakteri probiotik Bacillus terhadap A. hydrophila secara in vitro, dilanjutkan dengan aplikasi pada budidaya ikan lele dumbo (in vivo). Pada uji in vivo, penelitian terdiri atas lima perlakuan yaitu budidaya ikan lele dumbo dengan penambahan probiotik Bacillus P4I1 RifR, Bacillus P4I2 RifR, kombinasi probiotik Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), kontrol positif (K+; hanya ditambahkan A. hydrophila) dan kontrol negatif (K-; tanpa pemberian probiotik dan A. hydrophila). Ikan lele dumbo (13,35±2,80 g) dipelihara pada akuarium volume 40 L dengan kepadatan 30 ekor/akuarium selama 30 hari. Bakteri probiotik ditambahkan pada media pemeliharaan ikan setiap hari, sedangkan bakteri patogen A. hydrophila RifR (103 cfu/ mL) diberikan sekali pada awal pemeliharaan (kecuali pada kontrol negatif). Hasil penelitian menunjukkan bahwa konsentrasi terbaik pada penghambatan in vitro adalah dengan penambahan Bacillus 104 cfu/mL. Hasil uji in vivo menunjukkan perlakuan penambahan probiotik pada media budidaya efektif dapat menekan jumlah bakteri A. hydrophila, memperbaiki respons imun, dan meningkatkan kelangsungan hidup ikan lele dumbo dibanding kontrol positif. Perlakuan probiotik P4I1 RifR memberikan hasil terbaik dengan tingkat kelangsungan hidup tertinggi yaitu 92,23%. Kata kunci: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotik

Co-Authors . Sunarto A. Sunarma A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Anjarweni, Vika Atinia Annisa Astri Anggraeni ANTONIUS SUWANTO Antonius Suwanto Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Cahyawati, Nadia Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fadilah, Iin Nur Fahmi Rajab Fauzi, Arini Resti Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Marli, Marli Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Ratnadilla, Thamara Reza Samsudin Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rizkiyanti, Ita Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Saputri, Rika Ani Septiani, Ghita Ryan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suprianto, Dedi Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Taufiq Abdullah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wicaksono, Baref Agung Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yulianti, Susy Anisa Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad

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