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EFEK PROBIOTIK Bacillus megaterium PTB 1.4 DAN Pediococcus pentosaceus E2211 TERHADAP REPONS IMUN DAN KELANGSUNGAN HIDUP IKAN LELE (Clarias sp.) SELAMA UJI TANTANG Aeromonas hydrophila Hamka, Muhammad Subhan; Meryandini, Anja; Widanarni, Widanarni; Kurniaji, Ardana
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 3 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.03.9

Abstract

Penyakit bakterial pada kegiatan budidaya ikan sudah menjadi masalah yang sering dihadapi pembudidaya. Salah satu jenis bakteri yang menyebabkan penyakit pada ikan lele (Clarias sp.) adalah bakteri Aeromonas hydrophila. Tujuan penelitian ini untuk mengevaluasi efek pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap respons imun dan kelangsungan hidup ikan lele selama uji tantang dengan bakteri A. hyrophila. Probiotik yang digunakan adalah kombinasi 0,5% B. megaterium PTB 1.4 dan 0,5%  P. pentosaceus E2211. Pemberian probiotik dilakukan pada hari ke-0 hingga hari ke-30 melalui pakan. Uji tantang dilakukan pada hari ke-30 hingga hari ke-40. Hasil penelitian menunjukkan bahwa pemberian probiotik dapat berpengaruh terhadap respons imun dan kelangsungan hidup ikan lele dalam menekan infeksi A. hydrophila. Total eritrosit, hemoglobin, hematokrit, total leukosit, aktivitas fagositik dan aktivitas lisozim signifikan (P<0,05) teramati meningkat pada hari ke-35 dan menurun pada hari ke-40. Kelangsungan hidup ikan lele berhasil dipertahankan pada 87,62±4,83%. Total bakteri A. hydrophila meningkat pada hari ke-35 dan menurun pada hari ke-40. Pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada dosis 0,5% dapat menekan pertumbuhan bakteri A. hydrophila dan meningkatkan status kesehatan ikan lele.
EDUKASI PROSES REPRODUKSI UNTUK PEMBERDAYAAN PEMBUDIDAYA LELE: UPAYA MENINGKATKAN PRODUKTIVITAS BENIH DI KABUPATEN KEDIRI Alimuddin, Alimuddin; Widanarni, Widanarni; Sudrajat, Agus Oman; Nuryati, Sri; Arfah, Harton; Rahmi, Kurnia Anggraini
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2864

Abstract

Catfish (Clarias sp.) aquaculture in Kediri Regency, East Java, faces challenges due to the limited availability of high-quality broodstock and the lack of mastery in catfish spawning technologies, such as broodstock selection and the use of natural materials and probiotics to accelerate gonad maturation. These issues formed the basis for implementing a community empowerment program titled "Empowering Fish Farmers through Reproductive Process Education to Improve Catfish Seed Productivity in Kediri Regency." The program aimed to educate fish farmers on proper catfish breeding techniques, from broodstock selection to the use of probiotics and pine fruit extract to enhance gonad maturation and broodstock rematuration, through outreach activities. The community service program included public outreach to fish farming communities and discussions on program sustainability with the Kediri Regency Fisheries Department. Key stakeholders involved in the program included IPB University, the Fisheries Department of Kediri Regency, fisheries extension officers (Ministry of Marine Affairs and Fisheries), and catfish farmer groups in the region. The outreach began with a pre-test and concluded with a post-test to evaluate the program’s effectiveness. The materials covered included broodstock selection, application of pine fruit extract and probiotics for accelerating gonad maturation and rematuration. The community responded positively, as evidenced by an increase in average test scores from 78.42 (pre-test) to 92.63 (post-test), indicating that participants had gained a general understanding of catfish farming techniques and, through the outreach, comprehended the technology needed to produce high-quality broodstock.
Pathogenicity test of Vibrio parahaemolyticus in Pacific white shrimp Penaeus vannamei Widanarni; Kautsar, Badar; Wahjuningrum, Dinamella; Gustilatov, Muhamad
Journal of Vocational in Aquaculture (JAVA) Vol 1 No 1 (2024): NOVEMBER 2024
Publisher : College of Vocational Studies, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/java.v1i1.58786

Abstract

One of the common diseases affecting the Pacific white shrimp (Penaeus vannamei) culture is vibriosis, caused by infection with Vibrio species, including Vibrio parahaemolyticus. Certain strains of V. parahaemolyticus that carry the PirA and PirB toxins are responsible for causing acute hepatopancreatic necrosis disease (AHPND). This study aimed to assess the pathogenicity of V. parahaemolyticus in P. vannamei using bacterial isolates from different sources. A challenge test was conducted using P. vannamei with an average weight of 0.9±0.1 g, exposed to bacterial concentrations of 104 CFU/mL, as determined by lethal concentration 50% (LC50). The experiment followed a completely randomized design with three treatments and three replicates: a control group (K) of uninfected shrimp, shrimp infected with V. parahaemolyticus from Tasikmalaya, West Java (Vp-1), and shrimp infected with V. parahaemolyticus from Situbondo, East Java (Vp-2). Parameters observed included the confirmation of V. parahaemolyticus and AHPND via polymerase chain reaction (PCR), mortality rate, clinical symptoms, bacterial load, and immune response indicators, including total haemocyte count, phagocytic activity, respiratory burst, phenoloxidase activity, and histopathological examination of the hepatopancreas and intestines. Both Vp-1 and Vp-2 isolates were identified as V. parahaemolyticus AHPND strains, infecting shrimp with clinical signs such as pale hepatopancreas, empty intestines, and lethargic movement. Tissue damage, including extensive necrosis in the hepatopancreas and intestines, was observed, leading to mortality rates of 73.33-81.67% with an average time to death ranging from 24.28 to 65.44 hours postinfection.
PENINGKATAN PRODUKTIVITAS TAMBAK UDANG VANAME MELALUI PENGEMBANGAN SUMBERDAYA MANUSIA DI DESA KARANGSEWU, KECAMATAN GALUR, KABUPATEN KULON PROGO, DIY : Increasing the Productivity of White Shrimp Ponds Through Human Resources Development in Karangsewu Village, Galur District, Kulon Progo Regency, DIY Diatin, Iis; Budiardi, Tatag; Hadiroseyani, Yani; Effendi, Irzal; Widanarni, Widanarni; Vinasyam, Apriana; Astari, Belinda; Apriani, Ita
Jurnal Pepadu Vol 5 No 4 (2024): Jurnal PEPADU
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v5i4.5895

Abstract

Quality of human resources is a key factor in the success of cultivation. Farmers who have good technical knowledge and skills tend to be more successful in managing their ponds. Therefore, through training and mentoring programs, it is hoped that the knowledge and skills needed to increase shrimp pond productivity can be provided. The purpose of this activity is to improve farmers' understanding of effective and efficient white shrimp cultivation techniques, identify problems, and create networks between farmers to share experiences and information. The training was held in July 2024 in Karangsewu Village, Galur District, Kulon Progo Regency, D.I.Yogyakarta Province. The training attended by 35 participants consisting of white shrimp farmers, fisheries extension workers, and the surrounding community. After completing the training, participants received support in the form of mentoring for shrimp cultivation activities for three months. Pre-tests and post-tests were conducted to measure the increase in participants' knowledge. The results showed that the level of knowledge of participants increased by 6.64%. As many as 79% of participants strongly agreed that the training could improve their knowledge, 71% strongly agreed that the training they attended was relevant to their jobs, and 86% of participants stated that they could develop their potential after attending the program. The results of post-training monitoring showed that productivity of white shrimp pond increased from an average of 12 tons/ha to 23.16 tons/ha. This program not only increases productivity and business sustainability, but also has a positive impact on the economy of the surrounding community. There needs to be a follow-up program to improve shrimp farmers' understanding of financial management, marketing strategies, and the development of value-added products.
Effect of Dietary Canarium indicum L. on the Growth, Health, and Resistance of Asian Seabass Challenged with Vibrio alginolyticus Taufiq Abdullah; Wahjuningrum, Dinamella; Widanarni Widanarni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.43881

Abstract

Abstract Canarium indicum offers the potential of usage in aquaculture. This study aimed to evaluate the utilization of C. indicum on growth performance, health, and resistance of Asian seabass Lates calcalifer challenged with Vibrio alginolyticus. The study employed a completely randomized design with seven treatments, namely positive control (K+), negative control (K-), vitamin control (KVit), antibiotic control (KAnt), doses of 5 g kg-1 (D05), 10 g kg-1 (D10), and 20 g kg-1 C. indicum (D20). The test feed was prepared with a coating method and fed for 30 days. On the 31st day of rearing, a challenge test was conducted by injecting V. alginolyticus intramuscularly at 106 CFU mL-1. The results showed that C. indicum treatment for 30 days had a significantly different effect (P<0.05) on weight gain and average daily growth, while KVit and KAnt treatments were not different from the control. After the challenge test, all doses of C. indicum treatment, as well as KVit and KAnt treatments, showed survival significantly different (P<0.05) from that of the positive control, which ranged from 88.89% to 95.56% and was not significantly different (P>0.05) from the negative control. The survival rate condition is identical to the cumulative survival condition which shows the highest mortality found in the positive control. The administration of C. indicum at a dose of 5 g kg-1 to 20 g kg-1 improved the growth performance and prevented V. alginolyticus infection. Highlight Research C. indicum significantly increases the growth of Asian seabass. C. indicum significantly enhances the immune response to V. alginolyticus infection in Asian seabass. C. indicum can prevent V. alginolyticus infection as it resulted in a higher and cumulative survival than the positive control. C. indicum can be developed as an immunostimulant for the prevention of vibriosis in Asian seabass at doses of 5 g kg-1 to 20 g kg-1.
Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila Fauzi, Arini Resti; Yuhana, Munti; Widanarni, Widanarni; Setiawati, Mia; Afiff, Usamah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44079

Abstract

Abstract Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10⁶ CFU mL⁻1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection. Highlight Research Dayak onion has significant potential to improve nonspecific immunity in fish. Dayak onion improves hematology and immune response of Nile tilapia. Dayak onion can significantly regulate the immune system against A. hydrophila infection. Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei)) Widanarni, Widanarni; Sukenda, Sukenda; Septiani, Ghita Ryan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5041

Abstract

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.4930.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.930.025-6.970.019% and had higher values than controls (A and B) (P0.05). Meanwhile, feed conversion value in C and D (1.540.142 and 1.580.117) were lower than controls (P0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
PENGARUH STRUKTUR MODAL, KEBIJAKAN DIVIDEN DAN PROFITABIITAS TERHADAP NILAI PERUSAHAAN Marli, Marli; Anjarweni, Vika Atinia; Pudjiastuti, Widanarni
Dinamika Ekonomi: Jurnal Ekonomi dan Bisnis Vol 17 No 1 (2024): DINAMIKA EKONOMI Jurnal Ekonomi dan Bisnis Vol.17 No.1 Maret 2024
Publisher : Sekolah Tinggi Ilmu Ekonomi Nasional (STIENAS) Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53651/jdeb.v17i1.489

Abstract

Abstract, This study aims to investigate the impact of Capital Structure, Dividend Policy, and Profitability on the Firm Value of companies listed on the Indonesia Stock Exchange (IDX) within the LQ45 Index. The primary objective is to ascertain whether the assessment of these variables significantly influences Firm Value. A purposive sampling technique was employed to select a sample of 24 companies across various sectors for analysis. Quantitative data analysis was conducted using SPSS (Statistical Package for Social Science). The findings indicate that Capital Structure does not exhibit a significant effect on Firm Value, while Dividend Policy and Profitability both demonstrate a statistically significant impact. Moreover, the combined effect of Capital Structure, Dividend Policy, and Profitability significantly influences Firm Value.
Evaluation of human resources capacity, institutional and marketing in the Minapolitan Area, Merangin Regency, Jambi Province Suprianto, Dedi; Effendi, Irzal; Budiardi, Tatag; Widanarni, Widanarni; Diatin, Iis; Hadiroseyani, Yani
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 10: No. 1 (April, 2023)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v1i2.8632

Abstract

Evaluation of human resource development and institutional aspects is a process of providing information that can be used as a consideration to improve capabilities and be able to influence the implementation of community empowerment programs. The aimed of this study was to evaluate the capacity of human resources, institutions, and aquaculture marketing systems in the minapolitan area of Merangin Regency. The method of data collection was carried out using a structured interview method with the help of a questionnaire to 493 fish cultivating households (CH). Data were analyzed by multidimensional scaling and leverage analysis using Rapfish software. The results showed that the CH of catfish obtained the highest average index of 55.58, followed by the CH of gouramy at 53.60, the CH of tilapia at 54.57 and the CH of catfish obtained an average index of 54.71. This illustrates that the performance of catfish farmers in aquaculture production technology and management systems is better than the performance of other fish farmers. The results of the evaluation of all aspects of the capacity of human resources, institutions, and aquaculture marketing systems indicate that there are 3 aspects that are in the range of 50.10-75.00 with moderately sustainable status, namely; human resource development aspect with an average index value (AIV) of 68.64, technology aspect with an AIV of 52.98, and natural resources aspect with an AIV of 65.22. Meanwhile, there are 2 aspects that are in the range of 25.10-50.00 with less sustainable status, namely the institutional aspect with an AIV of 48.23 and the economic aspect with an AIV of 48.62.Keywords: Aquaculture; Institutional; Marketing; Sustainability
ADMINISTRATION OF Curcuma spp. EXTRACT TO CONTROL Aeromonas hydrophila INFECTION IN STRIPED CATFISH (Pangasianodon hypophthalmus) Sasanti, Ade Dwi; Widanarni, Widanarni; Sukenda, Sukenda; Yuhana, Munti; Wahjuningrum, Dinamella; Setiawati, Mia
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.23-36

Abstract

Curcuma spp. is a phytobiotic with potential application in fish farming. This study aimed to evaluate the potential of Curcuma zedoaria, Curcuma aeruginosa and Curcuma mangga extracts in striped catfish infected with Aeromonas hydrophila. The study used a complete randomized design (CRD) consisting of six treatments: KN (negative control), KP (positive control, fish infected without treatment), PE (C. zedoaria extract 6.25 g kg-1), HE (C. aeruginosa extract 6.25 g kg-1), ME (C. mangga extract 6.25 g kg-1) and KE (combination of C. zedoaria extract 2.1 g kg-1 + C. aeruginosa extract 2.1 g kg-1 + C. mangga extract 2.1 g kg-1). A total of 360 striped catfish, 10 ± 0.5 cm were kept in 18 aquariums measuring 50 × 40 × 35 cm3 and fed the treatment for 30 days. The challenge test was conducted on day 31 by injecting A. hydrophila suspension (106 colony-forming units (CFU) mL-1) intramuscularly into fish. The results showed that the treatment administered were able to stimulate the expression of interleukin-1â, interferon-ã 2a, 2b genes, increase the number of red and white blood cells, hematocrit, hemoglobin, phagocytosis activity, respiratory burst, reduce the population of A. hydrophila in the intestine, and reduce tissue damage in striped catfish. Striped catfish treated with the extracts showed significantly higher survival rates (p<0.05) compared to the positive control group. The survival rates were: KN (100.00 %), KP (53.33%), PE (93.33 %), HE (91.67 %), ME (93.33 %), and KE (88.33 %). In conclusion, the treatment administered were able to enhance the immune response and resistance of striped catfish infected with A. hydrophila.
Co-Authors . Sunarto A. Sunarma A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Anjarweni, Vika Atinia Annisa Astri Anggraeni ANTONIUS SUWANTO Antonius Suwanto Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Cahyawati, Nadia Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fadilah, Iin Nur Fahmi Rajab Fauzi, Arini Resti Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Marli, Marli Mas Tri Djoko Sunarno, Mas Tri Djoko Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Ratnadilla, Thamara Reza Samsudin Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rizkiyanti, Ita Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Saputri, Rika Ani Septiani, Ghita Ryan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suprianto, Dedi Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Taufiq Abdullah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wicaksono, Baref Agung Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yulianti, Susy Anisa Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad