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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Biosaintifika: Journal of Biology & Biology Education Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Penyuluhan Perikanan dan Kelautan J-ADIMAS (Jurnal Pengabdian kepada Masyarakat) Jurnal Abdi Insani Inspirasi Ekonomi : Jurnal Ekonomi Manajemen Jurnal ABM Mengabdi Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Rahmatan Lil’alamin Journal of Community Services (RLA) Jurnal Pepadu Jurnal Kedokteran Hewan Journal of Fisheries & Marine Journal of Vocational in Aquaculture (JAVA)
WIDANARNI WIDANARNI
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680
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Growth, immune responses, and resistance of vannamei shrimp fed with Lactobacillus paracasei probiotic and paraprobiotic and infected with Vibrio parahaemolyticus Nuri Kamaliah, Syarifah; Yuhana, Munti; Widanarni, Widanarni; Setiawati, Mia; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.199-210

Abstract

Vannamei shrimp is one of the most economically valuable aquaculture commodities in Indonesia. One of the pathogenic bacteria that is often found in vannamei shrimp farming is Vibrio parahaemolyticus. This study aimed to analyze the effectivity of Lactobacillus paracasei probiotics and paraprobiotics through feed with different cell densities on growth, and immune responses infected with V. parahaemolyticus. Vannamei shrimp of size 0.63 ± 0.01 were reared in containers with a stocking density of 15 shrimp per container and supplemented feed for 30 days. The research design consisted of six treatments, each with three replicates, namely (K-) feeding without supplements and injected with phosphate-buffered saline (PBS), (K+) feeding without supplements and infected with V. parahaemolyticus (104 CFU/mL), (PRI) feeding with 1% (v/w) probiotic L. paracasei with cell density of 106 CFU/mL, (PRII) 1% (v/w) probiotic L. paracasei cell density 109 CFU/mL, (PAI) 1% (v/w) paraprobiotic L. paracasei cell density 106 CFU/mL, (PAII) 1% (v/w) paraprobiotic L. paracasei cell density 109 CFU/mL. All treatments, except K-, were infected with V. parahaemolyticus (104 CFU/mL). Vannamei shrimp rearing was continued post the challenge test with V. parahaemolyticus which was conducted up to six days post injection. The results showed that feeding both probiotic and paraprobiotic L. paracasei through feed has improved growth, immune response, protein fat retention, and digestive enzyme activity of vannamei shrimp better than those of control. As the recommendation for the disease control of V. parahaemolyticus is feed supplementation with 1% (v/w) probiotic L. paracasei with cell density of 109 CFU/mL. Keywords: Lactobacillus paracasei, paraprobiotic, probiotic, vannamei shrimp, Vibrio parahaemolyticus ABSTRAK Udang vaname merupakan salah satu komoditas akuakultur yang bernilai ekonomis tinggi di Indonesia. Salah satu bakteri patogen yang sering ditemukan dalam budidaya udang vaname ialah bakteri Vibrio parahaemolyticus. Penelitian ini bertujuan untuk menganalisis pemberian probiotik dan paraprobiotik Lactobacillus paracasei melalui pakan dengan kepadatan sel berbeda terhadap pertumbuhan, dan respons imunitas yang diinfeksi V. parahaemolyticus. Udang vaname dengan ukuran 0.63 ± 0.01 gr dipelihara di dalam kontainer dengan padat tebar 15 ekor per wadah dan pemberian pakan bersuplemen selama 30 hari. Rancangan penelitian terdiri dari enam perlakuan, tiga ulangan, yaitu (K-) pemberian pakan tanpa suplemen dan diinjeksi PBS, (K+) pemberian pakan tanpa suplemen dan diinfeksi V. parahaemolyticus (104 CFU/mL), (PRI) pemberian pakan dengan probiotik L. paracasei kepadatan sel 106 CFU/mL dosis 1% (v/w), (PRII) probiotik L. paracasei kepadatan sel 109 CFU/mL dosis 1% (v/w), (PAI) paraprobiotik L. paracasei kepadatan sel 106 CFU/mL dosis 1% (v/w), (PAII) paraprobiotik L. paracasei kepadatan sel 109 CFU/mL dosis 1% (v/w), dan masing-masing diinfeksi V. parahaemolyticus (104 CFU/mL). Pemeliharaan udang vaname dilanjutkan setelah uji tantang dengan V. parahaemolyticus yang dilakukan hingga enam hari pasca injeksi. Hasil penelitian menunjukkan bahwa pemberian probiotik maupun paraprobiotik L. paracasei melalui pakan telah meningkatkan pertumbuhan, respons imun, retensi lemak protein, dan aktivitas enzim pencernaan udang vaname lebih baik dibandingkan kontrol. Untuk pengendalian V. parahaemolyticus diperoleh hasil terbaik dengan aplikasi probiotik L. paracasei dosis 1% (v/w) dengan kepadatan sel 109 CFU/mL. Kata Kunci: Lactobacillus paracasei, paraprobiotik, probiotik, udang vaname, Vibrio parahaemolyticus
Penguatan Growth Mindset untuk Optimalisasi Pengelolaan Desa Wisata Berkelanjutan di Toyomarto, Malang Rahmawati, Rina; Maria, Evi; Pudjiastuti, Widanarni; Aryani, Dwi Nita; Hariadi, Sugeng
J-ADIMAS (Jurnal Pengabdian Kepada Masyarakat) Vol 13, No 1 (2025)
Publisher : (STKIP) PGRI Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29100/j-adimas.v13i1.8509

Abstract

Pengelolaan desa wisata yang optimal memerlukan sinergitas antarorganisasi dan pola pikir yang adaptif terhadap perubahan. Kegiatan pengabdian kepada masyarakat ini bertujuan mengembangkan growth mindset pada pengelola Desa Wisata Toyomarto, Singosari, Kabupaten Malang. Kegiatan dilaksanakan pada 8 Januari 2025 dengan melibatkan 32 peserta dari PKK, staf desa, BUMDes, dan Pokdarwis. Metode pelaksanaan mencakup pelatihan partisipatif, diskusi kelompok terarah, simulasi manajemen, dan refleksi individual. Evaluasi dilakukan melalui pre-test dan post-test untuk mengukur peningkatan pemahaman peserta. Hasil menunjukkan peningkatan signifikan pada skor evaluasi dari 65% menjadi 85%, dengan 90% peserta menyatakan peningkatan kepercayaan diri dalam mengelola potensi desa wisata. Program berhasil membangun kesadaran kolektif tentang pentingnya harmonisasi organisasi dan strategi kolaboratif dalam pengembangan pariwisata berbasis komunitas. Kegiatan ini memberikan kontribusi praktis dalam penguatan kapasitas masyarakat desa melalui pendekatan psikologi positif yang dapat direplikasi di desa wisata lainnya.
COAL STOCKS: UNDERVALUED OR OVERHYPED VALUATION STUDY WITH EV/EBITDA: (Study On Coal Commodity Stock Group For The Period 2020-2024) Sudiarto, Edi; Pudjiastuti, Widanarni
Inspirasi Ekonomi : Jurnal Ekonomi Manajemen Vol. 7 No. 2 (2025): Inspirasi Ekonomi : Jurnal Ekonomi Manajemen
Publisher : Program Studi Manajemen, Fakultas Ekonomi dan Bisnis, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/ie.v7i2.9844

Abstract

This study evaluates the stock valuation of ITMG, BYAN, and PTBA using the EV/EBITDA method during the period 2020–2024 to determine whether the stocks are undervalued or overvalued . The data used comes from the company's financial statements and related publications. The analysis was carried out by comparing the EV/EBITDA value of each company to the industry average each year. The results of the study show that ITMG is consistently undervalued , with a lower EV/EBITDA ratio than the industry average. BYAN , on the other hand, tends to be overvalued , especially in the last three years with a ratio that is much higher than the industry average. PTBA experienced a change in trend, from being overvalued in 2020–2021 to being undervalued from 2022 to 2024. These results indicate that undervalued stocks such as ITMG and PTBA can be an investment opportunity for investors seeking attractive valuations, while BYAN's overvalued shares require further analysis related to market expectations and company growth. This study provides insight for investors in optimizing investment decisions in the coal sector.
Peran Literasi Keuangan Dalam Meningkatkan Usaha Masyarakat Disabilitas Perempuan Di Malang Raya Wiyarni, Wiyarni; Pudjiastuti, Widanarni; Sudjawoto, Eko; Wijaya, Sella Septian; Saputra, Damar Auliawan
Jurnal ABM Mengabdi Vol 12 No 1 (2025): Juni
Publisher : STIE Malangkucecwara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31966/jam.v12i1.1577

Abstract

In this era of globalization, financial literacy has become an essential skill for all levels of society to achieve economic independence. Moreover, financial literacy skills play an important role in supporting business success, especially for community groups who have limited access to information and economic opportunities, such as women with disabilities. The Community Service Program (PKM) is an effort to reach communities that have received little attention, especially universities and academics. This PKM aims to improve the businesses of women with disabilities in the Greater Malang area. Activities carried out include financial literacy training on the importance of separating personal or household transactions from business transactions in order to measure business progress. It is hoped that this training can improve the businesses of women with disabilities in Greater Malang.
EFEK PROBIOTIK Bacillus megaterium PTB 1.4 DAN Pediococcus pentosaceus E2211 TERHADAP REPONS IMUN DAN KELANGSUNGAN HIDUP IKAN LELE (Clarias sp.) SELAMA UJI TANTANG Aeromonas hydrophila Hamka, Muhammad Subhan; Meryandini, Anja; Widanarni, Widanarni; Kurniaji, Ardana
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 3 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.03.9

Abstract

Penyakit bakterial pada kegiatan budidaya ikan sudah menjadi masalah yang sering dihadapi pembudidaya. Salah satu jenis bakteri yang menyebabkan penyakit pada ikan lele (Clarias sp.) adalah bakteri Aeromonas hydrophila. Tujuan penelitian ini untuk mengevaluasi efek pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap respons imun dan kelangsungan hidup ikan lele selama uji tantang dengan bakteri A. hyrophila. Probiotik yang digunakan adalah kombinasi 0,5% B. megaterium PTB 1.4 dan 0,5%  P. pentosaceus E2211. Pemberian probiotik dilakukan pada hari ke-0 hingga hari ke-30 melalui pakan. Uji tantang dilakukan pada hari ke-30 hingga hari ke-40. Hasil penelitian menunjukkan bahwa pemberian probiotik dapat berpengaruh terhadap respons imun dan kelangsungan hidup ikan lele dalam menekan infeksi A. hydrophila. Total eritrosit, hemoglobin, hematokrit, total leukosit, aktivitas fagositik dan aktivitas lisozim signifikan (P<0,05) teramati meningkat pada hari ke-35 dan menurun pada hari ke-40. Kelangsungan hidup ikan lele berhasil dipertahankan pada 87,62±4,83%. Total bakteri A. hydrophila meningkat pada hari ke-35 dan menurun pada hari ke-40. Pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada dosis 0,5% dapat menekan pertumbuhan bakteri A. hydrophila dan meningkatkan status kesehatan ikan lele.
EDUKASI PROSES REPRODUKSI UNTUK PEMBERDAYAAN PEMBUDIDAYA LELE: UPAYA MENINGKATKAN PRODUKTIVITAS BENIH DI KABUPATEN KEDIRI Alimuddin, Alimuddin; Widanarni, Widanarni; Sudrajat, Agus Oman; Nuryati, Sri; Arfah, Harton; Rahmi, Kurnia Anggraini
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2864

Abstract

Catfish (Clarias sp.) aquaculture in Kediri Regency, East Java, faces challenges due to the limited availability of high-quality broodstock and the lack of mastery in catfish spawning technologies, such as broodstock selection and the use of natural materials and probiotics to accelerate gonad maturation. These issues formed the basis for implementing a community empowerment program titled "Empowering Fish Farmers through Reproductive Process Education to Improve Catfish Seed Productivity in Kediri Regency." The program aimed to educate fish farmers on proper catfish breeding techniques, from broodstock selection to the use of probiotics and pine fruit extract to enhance gonad maturation and broodstock rematuration, through outreach activities. The community service program included public outreach to fish farming communities and discussions on program sustainability with the Kediri Regency Fisheries Department. Key stakeholders involved in the program included IPB University, the Fisheries Department of Kediri Regency, fisheries extension officers (Ministry of Marine Affairs and Fisheries), and catfish farmer groups in the region. The outreach began with a pre-test and concluded with a post-test to evaluate the program’s effectiveness. The materials covered included broodstock selection, application of pine fruit extract and probiotics for accelerating gonad maturation and rematuration. The community responded positively, as evidenced by an increase in average test scores from 78.42 (pre-test) to 92.63 (post-test), indicating that participants had gained a general understanding of catfish farming techniques and, through the outreach, comprehended the technology needed to produce high-quality broodstock.
Pathogenicity test of Vibrio parahaemolyticus in Pacific white shrimp Penaeus vannamei Widanarni; Kautsar, Badar; Wahjuningrum, Dinamella; Gustilatov, Muhamad
Journal of Vocational in Aquaculture (JAVA) Vol 1 No 1 (2024): NOVEMBER 2024
Publisher : College of Vocational Studies, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/java.v1i1.58786

Abstract

One of the common diseases affecting the Pacific white shrimp (Penaeus vannamei) culture is vibriosis, caused by infection with Vibrio species, including Vibrio parahaemolyticus. Certain strains of V. parahaemolyticus that carry the PirA and PirB toxins are responsible for causing acute hepatopancreatic necrosis disease (AHPND). This study aimed to assess the pathogenicity of V. parahaemolyticus in P. vannamei using bacterial isolates from different sources. A challenge test was conducted using P. vannamei with an average weight of 0.9±0.1 g, exposed to bacterial concentrations of 104 CFU/mL, as determined by lethal concentration 50% (LC50). The experiment followed a completely randomized design with three treatments and three replicates: a control group (K) of uninfected shrimp, shrimp infected with V. parahaemolyticus from Tasikmalaya, West Java (Vp-1), and shrimp infected with V. parahaemolyticus from Situbondo, East Java (Vp-2). Parameters observed included the confirmation of V. parahaemolyticus and AHPND via polymerase chain reaction (PCR), mortality rate, clinical symptoms, bacterial load, and immune response indicators, including total haemocyte count, phagocytic activity, respiratory burst, phenoloxidase activity, and histopathological examination of the hepatopancreas and intestines. Both Vp-1 and Vp-2 isolates were identified as V. parahaemolyticus AHPND strains, infecting shrimp with clinical signs such as pale hepatopancreas, empty intestines, and lethargic movement. Tissue damage, including extensive necrosis in the hepatopancreas and intestines, was observed, leading to mortality rates of 73.33-81.67% with an average time to death ranging from 24.28 to 65.44 hours postinfection.
Co-Authors . Sunarto A. Sunarma A. SUWANTO A. Suwanto A.J. Sihombing Achmad Farouq Ade Dwi Sasanti ADNI OKTAVIANA Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alfabetian Harjuno Condro Haditomo Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Alit Brilliant Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Al Rosjidi Anis Zubaidah Anja Meryandini Anja Meryandini Annisa Astri Anggraeni Antonius Suwanto Apriani, Ita Ardana Kurniaji Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Asri Sutanti, Asri Asrido, Farhan Astari, Belinda Ayi Santika B. W. LAY Bagus Ansani Takwin Bako, Surandha Benny Heltonika Bunyamin Bunyamin Catur A. Pebrianto D. Augustine D. Djokosetiyanto D. Meha D. Shafruddin D.D. Mailana D.T. Soelistyowati Daniel Djokosetiyanto DEBY YUNIASARI DEDI JUSADI Dendi Hidayatullah, Dendi Dewi Nurhayati Dewi Rahmi DIAH AYU SATYARI UTAMI DIANA ELIZABETH WATURANGI Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Agung Saputra Dwi Nita Aryani E. Ayuzar Eddy Supriyono Edi Sudiarto Eko Sudjawoto Elizabeth Waturangi, Diana Enang Harris Enang Harris Enzeline, Valensia Evi Maria Fahmi Rajab Ferymon Mahulette Ferymon Mahulette, Ferymon Fiska Puspita Fitria Novianti Frid Agustinus Gentiga Muhammad Zairin Ghita Ryan Septiani Gustilatov, Muhamad H.J. Bugri Hamida Pattah Hamsah Hamsah Hamtini - Hamtini Hany Handajani Hariadi, Sugeng Harton Arfah Hasan Nasrullah Huda Salahudin Darusman Huda Shalahudin Darusman I. Effendi I. Tepu Iis Diatin Iis Sumartini Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irma Melati Irzal Effendi Istiqomah, Amalia Ita Apriani Jeanni Indah Noermala Julie Ekasari Julyantoro, Pande Gde Sasmita Kautsar, Badar Kukuh Nirmala Lastriliah, Mira Lilik Setiyaningsih Linuwih Aluh Prastiti M. Tri Djoko Sunarno M. Yusuf Arifin M. Zairin Junior M.A. Lidaenni M.S. Arifin Maulana, Fajar Mia Setiawati Mohammad Faizal Ulkhaq MUHAMMAD AGUS SUPRAYUDI Muhammad Junior Zairin Muhammad Subhan Hamka Muhammad Zairin Jr. Mulyasari Mulyasari Mulyasari Mulyasari MUNTI YUHANA Nasrullah, Hasan NISA RACHMANIA MUBARIK Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah O. Carman Ode, Inem Prassetyo Dwi Dhany Wijaya Puguh Widagdo Putra, The Best Akbar Esa Rahmi, Kurnia Anggraini Rakhmawati, Rakhmawati, Ramadhani, Dian Eka Rifqah Pratiwi Rina Rahmawati, Rina Rio Yusufi Subhan Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Ruku Ratu Borut S. Sarah Safira Qisthina Ayuningtyas, Safira Qisthina Salamah Salamah Samsu Adi Rahman Saputra, Damar Auliawan Siregar, Khoirotun Nisa SITI MARYAM Siti Munfaqiroh Sri Nuryati Sri Nuryati Suci antoro Sudrajat, R Herman Sugiyo Hadi Pranoto Sujaka Nugraha Sujono Sujono Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda SUKENDA SUKENDA Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Suryadi Saputra Tambun, Andreas Tatag Budiardi Tatik Mufidah Tatik Mufidah Titin Kurniasih Tri Heru Prihadi Tri Rezeki, Nanda Tsani Untsa, Agista Turnip, Enita Romasni Vinasyam, Apriana W. Efiyanti Wahyu Afrilasari WAODE MUNAENI Wasmen Manalu Wida Lesmanawati Wijaya, Sella Septian Windu Sukenda Wira H Saputra Wiyarni Wiyarni Y. Hadiroseyani Yani Aryati Yanti Inneke Nababan Yeni Elisdiana Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yuke Eliyani Yuke Eliyani Yunarty Yunarty Zafril Imran Azwar Zairin Jr., Muhammad